Hitachi UH5300 User manual

INSTRUCTION MANUAL
AUTO SIPPER
FOR
MODEL UH5300 SPECTROPHOTOMETER
Hitachi High-Technologies Corporation
24-14, Nishi-Shimbashi 1-chome, Minato-ku, Tokyo, Japan
Copyright C Hitachi High-Technologies Corporation 2013. 1st Edition, 2013
All rights reserved. Printed in Japan. Part No. 3J1-9021 HM-N (HMS)

Be sure to read through and understand the following points
with regard to this manual.
1. Information contained in this document is subject to
change without notice for improvement.
2. This manual is copyrighted by Hitachi High-Technologies
Corporation with all rights reserved.
No part of this manual may be reproduced, transmitted
or disclosed to a third party in any form or by any means
without the express written permission of Hitachi
High-Technologies Corporation.
3. Hitachi High-Technologies Corporation assumes no
liability for any direct, indirect, or consequential damages
arising from use not described in this manual.
Utmost care must be exercised when using the
instrument.
4. This document does not provide any warranty or
permission for industrial properties or any rights to grant
license lawfully and without infringement.

PREFACE
We thank you for purchasing the Auto Sipper for the Model UH5300
Spectrophotometer.
The Auto Sipper is an instrument that is used in combination with the
Model UH5300 Spectrophotometer to measure the absorbance etc.
of samples.
Note that samples that may have been infected with bacteria or
viruses are not applicable to this instrument.
This instrument is intended for use by persons having a basic
knowledge of chemical analysis procedures.
Keep in mind that improper use of analytical instruments,
chemicals or samples would result not only in wrong analytical
data but also in consequences adverse to safety. Note that it is
allowed only for persons having a basic knowledge of chemical
analysis procedures to use this instrument.
Please read this instruction manual carefully before attempting
operation and acquaint yourself with this product for its correct use.
Be sure to read the Model UH5300 Spectrophotometer’s instruction
manual also to ensure correct use of this instrument.
ABOUT THIS INSTRUCTION MANUAL
This instruction manual has been prepared for users of the Auto
Sipper for the Model UH5300 Spectrophotometer. The operating
procedures and maintenance/inspection instructions for the
instrument are contained in this manual.
First of all, be sure to read “IMPORTANT” and “SAFETY
SUMMARY” at the beginning of this manual.
Also read the Model UH5300 Spectrophotometer’s instruction
manual (both maintenance manual and operation manual).

IMPORTANT - 1
IMPORTANT
Product Warranty
This product is warranted to be free from defects in material or
workmanship under normal use within the product specifications
indicated in this manual and under conditions given below.
This warranty is void if the instrument is not used according to the
instruction manual.
(1) Scope of Warranty
Any parts that prove to be defective in design or workmanship
during the warranty period will be repaired, adjusted or replaced
without charge. Note that a substitute part may be used for
repair, or replacement with an equivalent product may be made
instead of repair.
The manufacturer assumes no liability for any damage to data or
application software due to any possible fault or failure of this
instrument.
(2) Warranty Period
One year from the date of initial installation
(In case a separate warranty document has been issued, the
warranty period indicated in it takes precedence over the above
period)
(3) Limitations and Exclusions on Warranty
Note that the following cases are excluded from the scope of
this warranty, i.e., these cases are beyond the coverage of
free-of-charge repair even during the warranty period indicated
above.
(a) Failure due to operation at a place not meeting the
installation requirements specified by the manufacturer.
(b) Failure due to use of power supply voltage/frequency other
than specified by the manufacturer or due to abnormality in
power supply.
(c) Corrosion or deterioration of the piping due to impurities
contained in gas, compressed air or cooling water supplied
by the user.

IMPORTANT - 2
(d) Corrosion of the electric circuits or deterioration of the
optical elements due to highly corrosive atmospheric gas.
(e) Failure due to use of hardware, software or spare parts not
supplied by the manufacturer.
(f) Failure due to use or usage not described in the manual or
improper repair not approved by the manufacturer.
(g) Failure due to maintenance or repair by other than service
engineer qualified by the manufacture.
(h) Failure due to relocation or transport after initial installation.
(i) After disassembly, modification or relocation not approved
by the manufacturer.
(j) Failure due to acts of God, including fire, earthquake,
storm, flood, lightning, social disturbance, riot, crime,
insurrection, terrorism, war (declared or undeclared),
radioactive pollution, contamination with harmful
substances, etc.
(k) Failure of the hardware, or damage to the system software,
application software or data due to computer virus infection.
(l) After disposal of this instrument, after its resale without prior
approval from the manufacturer, consumable parts, and
failure of any part that have reached the end of its service
life.
(m) Failure due to a life-limited part that has exceeded the end
of its useful lifetime.

IMPORTANT - 3
(4) Disclaimer of Warranty
THE MANUFACTURER MAKES NO WARRANTIES, EITHER
EXPRESS OR IMPLIED, EXCEPT AS PROVIDED HEREIN,
INCLUDING WITHOUT LIMITATION THEREOF, WARRANTIES
AS TO MARKETABILITY, MERCHANTABILITY, FOR A
PARTICULAR PURPOSE OR USE, OR AGAINST
INFRINGEMENT OF ANY PATENT. IN NO EVENT SHALL THE
MANUFACTURER BE LIABLE FOR ANY DIRECT,
INCIDENTAL OR CONSEQUENTIAL DAMAGES OF ANY
NATURE, OR LOSSES OR EXPENSES RESULTING FROM
ANY DEFECTIVE PRODUCT OR THE USE OF ANY
PRODUCT.
NO ORAL OR WRITTEN INFORMATION OR ADVICE GIVEN
BY THE MANUFACTURER, ITS DEALERS, DISTRIBUTORS,
AGENTS OR EMPLOYEES SHALL CREATE A WARRANTY
OR IN ANY WAY INCREASE THE SCOPE OF THIS
WARRANTY.
Service Life of This Instrument
This instrument has a useful service life of seven years after the date
of its initial use (installation), which is estimated under the condition
that periodic maintenance, checkup, replacement of life-limited parts,
and repair of worn parts are carried out as specified in the present
instruction manual.
(In use of the instrument under standard operating conditions
(8 h/day, 20 /month))
For using the instrument beyond the useful service life, it shall be
checked for safety by Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-Technologies
Corporation sales representative. (This safety check will be available
on a chargeable basis.)
If use of the instrument is continued without receiving the safety
check, the instrument might become faulty and cause a danger.
Note that replacement may be recommended as a result of the
safety check.

IMPORTANT - 4
Installation/Relocation and After-sale Technical Service
(1) Installation and Relocation
(a) If you intend to install the instrument by yourself,
carefully read this instruction manual and the instruction
manual of the Model UH5300 Spectrophotometer
before doing so.
(b) Before installation, the user shall make preparations for
satisfying the installation requirements in accordance
with this instruction manual.
(c) If relocation becomes necessary after initial installation
(delivery), please contact the dealer from whom you
purchased the instrument or our designated service
company.
(2) After-sales Service
(a) For after-sales service, contact our sales representative
or service office of our sales representative.
(b) For service after the warranty period, consult our sales
representative or service office of our sales
representative with regard to a maintenance and
inspection service contract. (Service will be available
on a chargeable basis.)
(c) The maintenance and consumables of the instrument
can be supplied within the useful service life of the
instrument (7 years). Even after the period of useful
service life, the parts and units can be supplied (within
10 years after the date of initial use) so far as they are
obtainable. However, this measure will not lead to an
extension of the 7-year useful service life which is
assured by the manufacturer.
And, if a part or unit is unavailable due to the
discontinuance of its manufacture, a substitute part or
unit may be supplied, for which we request your
understanding.

IMPORTANT - 5
(d) It may be impossible to supply the main unit
components other than the maintenance parts and
consumables due to the discontinuance of main-unit
manufacture, etc. If the instrument becomes faulty, it
might be irreparable due to lack of such components.
In this case, the user is requested to stop operation and
replace the instrument with a new one.
Technical Seminars and Training for Customers
We offer technical seminars and training courses at either our or
user's facilities to ensure proper and safe operation of the analytical
instrument to its full performance.
For further information, contact our sales representative. (Applicants
will be charged.)
Caution on Disposal of Instrument
Although at present the instrument does not use materials that would
directly cause environmental disruption, the environmental protection
laws and regulations may be revised or amended. When planning
to dispose of the instrument, therefore, be sure to check the latest
issues of the relevant laws and regulations or consult our sales
representative.
Other Precautions
Handling of Chemicals and Samples
(1) The user is responsible for following the relevant legal standards
and regulations in handling, storage, and disposal of chemicals
and samples used in analytical operations with this instrument.
(2) Reagents, standard solutions and accuracy-control samples
shall be handled, stored and discarded as instructed by the
respective suppliers.
(3) Samples that may have been infected with bacteria or viruses
are not applicable to the instrument.)

SAFETY - 1
SAFETY SUMMARY
General Safety Guidelines
Before using the Auto Sipper for the Hitachi Model UH5300
Spectrophotometer, be sure to read the following safety instructions
carefully.
The hazard warnings which appear on the warning labels on the
product or in the manual have one of the following alert headings
consisting of an alert symbol and a signal word DANGER,
WARNING or CAUTION.
: Safety alert symbol used for calling attention to a
potential hazard which could cause personal
injury.
To avoid possible injury or death, observe all the
safety messages following this symbol.
DANGER : Indicates an imminently hazardous situation
which, if not avoided, will result in death or
serious injury.
(This warning does not apply to this product.)
WARNING: Indicates a potentially hazardous situation which,
if not avoided, can result in death or serious
injury.
(This warning does not apply to this product.)
CAUTION : Indicates a hazardous situation which, if not
avoided, can result in minor or moderate injury.
NOTICE : Indicates a hazardous situation which, if not
avoided, can result in damage to property.
In addition, the following signal word is used to indicate instructions
for ensuring proper use of the product.
NOTE : Indicates an instruction for ensuring correct use of the
product and accurate analysis therewith.

SAFETY - 2
SAFETY SUMMARY
Common Safety Precautions
Prior to Use
Before using the instrument, be sure to read this instruction
manual carefully to attain a full understanding of its operations.
Keep the instruction manual handy nearby so it can be referred to
whenever needed.
Be sure to observe the procedures specified in the manual.
Be sure to understand and follow all the safety instructions given
in the manual.
Be sure to observe all the hazard warnings attached to the
instrument or provided in the manual. Failure to do so could result
in personal injury or damage to the instrument.
Be sure to follow all the methods of use instructed in the manual
for proper application of the product.
Absolutely avoid modifying the product, using non-specified parts,
or removing safety devices as it could be hazardous.
Do not perform any operation or action other than described in the
manual.
On occurrence of any trouble in the instrument, notify your nearest
Hitachi High-Technologies Corporation sales representative or
service office of Hitachi High-Technologies Corporation sales
representative.
When using chemicals for the instrument, be sure to provide
proper ventilation of the room. Inadequate ventilation could
endanger human health.

SAFETY - 3
SAFETY SUMMARY
Common Safety Precautions (Continued)
Keep in mind that the hazard warnings in the manuals or on the
product cannot cover every possible case, as it is impossible to
predict and evaluate all circumstances beforehand. Always be
alert and use your common sense.
Wear appropriate protective equipment when using chemicals. In
the case of accidental contact with the skin or ingestion, refer to
the Material Safety Data Sheet (MSDS) to take first-aid action and
seek medical care.
In Use
If an abnormality such as unusual noise, odor, fuming or gas
leakage occurs during operation of the instrument, immediately
disconnect power to the instrument, and take proper safety
measures as required. Then, notify our designated service
company.
Installation, Maintenance, and Relocation
Upon delivery of the product, check the contents of the package to
see if any item is lost, referring to “1.2.2 Check against Packing
List” in this manual. If by any chance the instrument is operated
with any one of the standard parts not equipped, a failure could
occur to result in a hazardous condition.
If any item is missing or damaged or if you have any question,
notify the installation personnel at site or your nearest Hitachi
High-Technologies Corporation sales representative or service
office of Hitachi High-Technologies Corporation sales
representative.

SAFETY - 4
SAFETY SUMMARY
Common Safety Precautions (Continued)
The maintenance and checkup procedures to be performed by
users are limited to those described in the manual only. When
performing maintenance and checkup procedures described in the
manual, obtain a clear understanding of them. Do not perform
maintenance and checkup procedures other than described in the
instruction manual. Otherwise failure of the instrument or personal
injuries may result.
After installation, do not relocate the instrument. If the instrument
is relocated, vibration or impact to be applied during relocation
could cause a malfunction in the optical components that have
been adjusted precisely.
If any warning/caution label has become illegible due to
deterioration with age or it has been damaged due to any cause,
notify your nearest Hitachi High-Technologies Corporation sales
representative or service office of Hitachi High-Technologies
Corporation sales representative for replacement with a new one.
The parts having a useful lifetime indicated in this manual must be
replaced periodically as specified. If the instrument is operated
though the replacement of life-limited parts has already been
required, the instrument might become faulty due to part
deterioration, etc., causing leak, fuming, combustion or the like
trouble on safety.
For other than the replacement procedures instructed in this
manual, contact your nearest Hitachi High-Technologies
Corporation sales representative or service office of Hitachi
High-Technologies Corporation sales representative.
For reducing a risk of trouble occurrence due to physical
deterioration, it is requested to carry out the safety check
(available on a chargeable basis) or replacement with a new one
when the instrument has reached the end of its useful service life.

SAFETY - 5
SAFETY SUMMARY
Safety Instructions in This Manual
Shown below are safety instructions contained in this manual and
their relevant sections in it.
DANGER Indications
The indication “ DANGER” does not apply to this product.
WARNING Indications
The indication “ WARNING” does not apply to this product.
CAUTION Indications
Injury due to Entanglement
Hands or hair could be entangled in the peristaltic pump. Be sure
to close the cover of the sample compartment when operating the
Auto Sipper. (Section 3.1)
Fatigue due to Long Hours of Operation
In operating the instrument watching the display, a long hour
watching in the same posture can build up fatigue in the eyes or
body. For your health, when operating the instrument for long
hours, take a break 10 to 15 minutes every hour or so to rest your
eyes and body. (Instruction manual for the spectrophotometer)

SAFETY - 6
SAFETY SUMMARY
NOTICE
Disposal of Waste Solution
Be sure to collect waste solution and treat it for proper disposal in
accordance with the relevant laws and regulations regarding water
pollution control and sewage treatment. Improper treatment of
waste solution may result in environmental pollution. Additionally, it
could lead to a penalty.
Accuracy and Precision of Measured Values
Carry out periodic inspection and check whether the system is
operating normally. Additionally, perform measurement of control
samples when necessary.
Electricity
Make sure that the power supply to the spectrophotometer is
100 V AC, 1 kVA or more (50 or 60 Hz).
Be sure to prepare a grounding wire together with power cables
and confirm that the wire has a ground resistance of 100 or less
(Class D grounding construction in Electric Facility Technical
Standards).
The high voltage circuit is used inside the spectrophotometer. To
prevent electric shock, do not open covers during operation unless
otherwise necessary.

SAFETY - 7
SAFETY SUMMARY
NOTICE (Continued)
Power Interruption
A power failure or momentary voltage drop of the power supply
due to lightning, etc. may cause failure of the personal computer
used with the instrument and also damages the system software,
application software and other data.
To avoid such problems, it is recommended to use an AC
uninterruptible power source (stated according to the Japanese
Electronic Industry Development Association guidelines for
protection against momentary power voltage drop in personal
computers).

- i -
CONTENTS
PREFACE
ABOUT THIS INSTRUCTION MANUAL
IMPORTANT ......................................................................................................IMPORTANT-1
Product Warranty.........................................................IMPORTANT-1
Service Life of This Instrument.....................................IMPORTANT-3
Installation/Relocation and After-sale
Technical Service.........................................................IMPORTANT-4
Technical Seminars and Training for Customers..........IMPORTANT-5
Caution on Disposal of Instrument...............................IMPORTANT-5
Other Precautions........................................................IMPORTANT-5
SAFETY SUMMARY........................................................................................ SAFETY-1
General Safety Guidelines.......................................... SAFETY-1
Common Safety Precautions...................................... SAFETY-2
Safety Instructions in This Manual.............................. SAFETY-5
DANGER Indications........................................... SAFETY-5
WARNING Indications......................................... SAFETY-5
CAUTION Indications.......................................... SAFETY-5
Injury due to Entanglement.................................. SAFETY-5
Fatigue due to Long Hours of Operation.............. SAFETY-5
NOTICE...................................................................... SAFETY-6
1 INSTALLATION ...........................................................................................................1- 1
1.1 Outline................................................................................1- 1
1.2 Checking before Mounting Auto Sipper...............................1- 1
1.2.1 Installation Conditions .............................................1- 1
1.2.2 Checking against Packing List.................................1- 3
1.3 Mounting Auto Sipper in Sample Compartment...................1- 4
1.3.1 Dismounting 6 Cell Turret........................................1- 4
1.3.2 Dismounting Cell Holder for Reference ...................1- 4
1.3.3 Removing Sample Compartment Front Cover.........1- 5
1.3.4 Attaching Front Cover for Accessory Device............1- 6
1.3.5 Attaching Coupling Shaft.........................................1- 7
1.3.6 Mounting Auto Sipper..............................................1- 9
1.4 Reverse Process to Change Auto Sipper to 6 Cell Turret....1-16
1.4.1 Unplugging power cord............................................1-16
1.4.2 Dismounting Auto Sipper.........................................1-16
1.4.3 Detaching Coupling Shaft........................................1-19
1.4.4 Detaching Front Panel.............................................1-20
1.4.5 Attaching Sample Compartment Front Cover ..........1-20
1.4.6 Mounting Cell Holder for Reference.........................1-22
1.4.7 Mounting 6 Cell Turret.............................................1-23

- ii -
2 FUNCTIONS...................................................................................................................2-1
2.1 Application............................................................................2-1
2.2 Mechanism ...........................................................................2-1
2.2.1 Operational Mechanism.............................................2-1
2.2.2 Details of Measurement Section and
Liquid Delivery Mechanism........................................2-2
2.2.3 Setting Peristaltic Tube..............................................2-4
2.2.4 Releasing Peristaltic Tube.........................................2-7
2.3 Details of Operation Keys, etc...............................................2-7
2.3.1 Sipping Lever and SIPPING Lamp............................2-7
2.3.2 RETURN Key............................................................2-8
2.3.3 WASH Key................................................................2-8
3 OPERATION ................................................................................................................3- 1
3.1 Preparation.........................................................................3- 1
3.2 Setting Measurement Conditions ........................................3- 2
3.2.1 Selecting Measurement Item...................................3- 2
3.2.2 Setting Auto Sipper..................................................3- 3
3.3 Measurement......................................................................3- 5
3.3.1 Precautions for Measurement..................................3- 5
3.3.2 Concentration Measurement...................................3- 6
3.3.3 Wavelength Scan ....................................................3- 8
3.3.4 Time Scan...............................................................3- 9
3.3.5 Monitor Measurement .............................................3-10
3.4 Checking of Sipping Amount...............................................3-12
3.4.1 Specification to be Checked....................................3-12
3.4.2 Signs for Replacement of Peristaltic Tube...............3-12
3.4.3 Checking Conditions ...............................................3-12
3.4.4 Checking Procedure................................................3-12
3.5 After Measurement..............................................................3-13
3.5.1 Cleaning Flow Cell ..................................................3-13
3.6 Specifications......................................................................3-14
4 MAINTENANCE.............................................................................................................4-1
4.1 Periodic Maintenance............................................................4-1
4.1.1 Precautions for Measurement....................................4-1
4.2 Maintenance of Peristaltic Tube ............................................4-3
4.2.1 About Peristaltic Tube................................................4-3
4.2.2 Peristaltic Tube Replacement Procedure...................4-3
4.2.3 Checking Chemical Resistance of
Peristaltic Tube..........................................................4-6
4.3 Precautions on Using Maintenance Menu.............................4-8
4.4 Replacement Parts................................................................4-8
4.4.1 Consumables............................................................4-8
4.4.2 Timely Replacement Item..........................................4-8
4.5 Troubleshooting ....................................................................4-9

1.2.1
1 - 1
1 INSTALLATION
1.1 Outline
The Auto Sipper is an instrument that is incorporated in the sample
compartment section of the Model UH5300 Spectrophotometer to
measure the absorbance or permeability of samples. Sample liquid is
sipped through the peristaltic pump into the flow cell in the Auto
Sipper. The sipping amount of sample can be adjusted according to
the time of rotation of the peristaltic pump.
Fig. 1-1 shows an external view of the Auto Sipper.
Fig. 1-1 External View of Auto Sipper
1.2 Checking before Mounting Auto Sipper
1.2.1 Installation Conditions
(1) Service temperature: 15 to 35°C
For measurement in the most stable conditions, it is
recommended to install the instrument in a room which is
air-conditioned at 20 to 25°C.
(2) Service humidity: 25 to 80% RH
(Condensation is not allowed. Where the ambient temperature is
30°C or more, control the relative humidity to 70% RH or below.)
(3) Storage temperature: -20 to 60°C
(4) Storage humidity: 5 to 95% RH

1.2 Checking before Mounting Auto Sipper
1 - 2
(5) Atmospheric gas
(a) No corrosive gases such as acidic or alkaline gas that
heavily attack metals shall exist in ambience.
(b) No coating-dissolving gasses such as gas of organic
solvent (particularly benzine and thinner) that dissolve
coating shall exist in ambience.
(6) Other general precautions
(a) Do not place in the direct sunlight.
(b) No strong vibrations or shocks that human body feels shall
be applied.
(c) No heat-generators such as gas burners, electric heaters,
or ovens that cause the instrument to heat up (to 70°C or
more) shall exist nearby.
(d) The instrument shall not be placed close to equipment
(such as electric welders, high frequency electric furnace,
and pole transformers) that generates strong electric field or
magnetic field.
(e) No excessive dusts or litters shall exist.
(f) No sharp variation of the power supply voltage shall occur.
(g) Do not turn on or off frequently the power source of motors
(such as for stirrers and vibrators) that does not have noise
prevention devices when such motors are connected with
the same power supply line to which the main unit of the
spectrophotometer is connected.
(h) Do not place sample material on the main unit of the
spectrophotometer. Any sample spilled on the instrument
may cause it to malfunction.

1.2.2
1 - 3
1.2.2 Checking against Packing List
Upon completion of unpacking, check the contents of delivery against
the attached packing list. If any part is missing or damaged, or if you
have any question, contact the nearest sales representative or our
service engineer dispatched for the installation. (Do not touch directly
or breathe on the flow cell while unpacking.)
Part No. Part Name Q'ty
3J1-0400 Auto Sipper Main Unit 1
3J1-3214 Front Panel 1
775-1376 Rinsing Liquid 1
3J1-3040 Peristaltic Tubes (5 pcs.) 1
3J1-3066 Coupling Shaft 1
N315006 Hexagonal Wrench <M6> 1
171-6129 Sandpaper 1
Fig. 1-2 Auto Sipper Configuration
Rinsing Liquid
Part No.: 775-1376
Peristaltic Tubes
(5 pcs.)
Part No.: 3J1-3040
Auto Sipper Main Unit
Part No.: 3J1-0400
Hexagonal Wrench
<M6>
Part No.: N315006
Front Panel
Part No.: 3J1-3214
Sandpaper
Part No.: 171-6129
Coupling Shaft
Part No.:
3J1-3066