Invitrogen Bandmate Automated Western Blot Processor User manual

For Research Use Only. Not for use in diagnostic procedures.
Bandmate™Automated Western Blot
Processor
USER GUIDE
For automation of western blot processing
Catalog Numbers BW1000
Publication Number MAN0018722
Revision B.0

Thermo Fisher Scientific | 3747 N. Meridian Road | Rockford, Illinois 61101 USA
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The information in this guide is subject to change without notice.
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©2020 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■Bandmate™Automated Western Processor ........................................ 4
Product description ............................................................. 4
Contents ....................................................................... 5
Operate the Bandmate™Automated Western Blot Processor ......................... 7
Set up the system .......................................................... 7
Program the processor ...................................................... 7
Run the processor .......................................................... 9
Clean the processor ........................................................ 10
General maintenance ....................................................... 10
Instrument dimensions and specifications .................................... 12
Troubleshooting ................................................................ 13
Limited product warranty ........................................................ 13
■Safety ................................................................................. 14
Symbols on this instrument ..................................................... 14
Standard safety symbols ................................................... 15
Control and connection symbols ............................................. 16
Conformity symbols ........................................................ 16
Safety information for instruments not manufactured by Thermo Fisher Scientific ...... 17
Instrument safety .............................................................. 17
General ................................................................... 17
Physical injury ............................................................. 18
Electrical safety ........................................................... 19
Cleaning and decontamination .............................................. 20
Instrument component and accessory disposal ................................ 21
Safety and electromagnetic compatibility (EMC) standards .......................... 21
Safety standards .......................................................... 21
EMC standards ............................................................ 21
Environmental design standards ............................................. 22
Chemical safety ................................................................ 23
Biological hazard safety ......................................................... 25
Bandmate™Automated Western Blot Processor User Guide 3

Bandmate™Automated Western
Processor
Product description
The Invitrogen™Bandmate™Automated Western Blot Processor is a self-contained
western blot processing system that can process up to 4 mini blots or 2 midi blots
at a time. The instrument has built-in traditional programs, but permits custom
programs for incubation and wash times. Antibodies and reagents are directly
streamed into incubation trays, which eliminates cross-contamination between
reagents. The curved mini and midi trays allow for even processing of blots with
minimal antibody volumes; minimally 3.5 mL primary antibody for mini blots and
7.5 mL for midi blots.
1
6
2
3
4
5
7
8
Figure 1 Instrument overview
1Reagent tubes (antibody reservoirs)
2Wash buer tubing
3Rocker cradle
4Tray cover
5Sample tray
6Reagent slider
7Waste and recovery funnel tray
8Waste tank compartment (behind door
panel)
4Bandmate™Automated Western Blot Processor User Guide

Table 1 Program icons
Icon Function Icon Function
Accept Fill
Back Home
Cancel (Stop) Pause
Continue Run
Drain Save
Edit
Contents
Product Product No. Contents
Bandmate™Automated
Western Blot Processor BW1000
Processor Station
2 L Buer Bottle
Buer Hose w/ Quick-
connect Coupling
Interior Siphon Hose for
Buer Bottle
Reagent Tubes
50 mL Recovery Tubes
Mini Tray
Midi Tray
Tray Cover
Funnel Tray
Waste Tray
Quick Reference Guide
Bandmate™Automated Western Processor
Contents
Bandmate™Automated Western Blot Processor User Guide 5

Table 2 Available for separate purchase
Product Product No.
Reagent Reservoirs BW0100
Mini Tray BW0010
Midi Tray BW0020
Waste Tray BW0030
Funnel Tray BW0040
Tray Cover BW0050
2 L Buer Bottle BW0060
Table 3 Accessory products
Product Product No.
Pierce™Clear Milk Blocking Buer (10X) 37587
Blocker FL Fluorescent Blocking Buer (10X) 37565
Blocker BSA (10X) in PBS 37525
Blocker BSA (10X) in TBS 37520
SuperSignal™West Pico PLUS Chemiluminescent
Substrate
34579
SuperSignal™West Atto Ultimate Sensitivity
Substrate
A38555
Pierce™Reversible Protein Stain Kit for
Nitrocellulose Membranes
24580
Pierce™Reversible Protein Stain Kit for PVDF
Membranes
24585
Bandmate™Automated Western Processor
Contents
6Bandmate™Automated Western Blot Processor User Guide

Operate the Bandmate™Automated Western Blot Processor
Set up the system
1. Set up the processor on a stable, level lab bench.
2. Open the door and remove the funnel tray.
3. Replace the funnel tray so that it hooks onto the catch at the back of the unit.
Close the door.
4. Attach the wash buer hose to the connector located in the back of the
instrument and attach the other end to the buer bottle using the quick-connect
fitting.
5. Plug the power supply into the power jack on the back of the instrument and
connect the plug into a wall outlet.
Program the processor
•Store up to 8 programs.
•All incubation steps can be set from 1 second to 96 hours.
•Volume range for washes is between 10 mL to 75 mL for mini gels and 10 mL to
150 mL for midi gels.
•We recommend using 20-30 mL per wash for mini gels and 30-50 mL for midi
gels.
•Recommended antibody volumes are ≥ 3.5 mL for mini blots and ≥ 7.5 mL for
midi blots.
•Maximum number of given steps in a protocol is 9.
•Recommended rocks per minute for incubation steps is 15 rpm. Recommended
rocks per minute for wash steps is 20 rpm.
1. From the home screen, select Programs. A list of programs, with default times
will appear. The Standard Western Blotting Protocol may be used for guidance
and as a template. Select the desired program using the dial. Press Edit.
2. Enter a name for the custom protocol. Select letters and numbers by turning
the dial. Use the cluster of 4 buttons at the lower left of the screen to navigate
between characters and delete characters or add spaces. When finished, press
Save to save the program name, and then press Continue .
3. Select whether the protocol will be used with a mini tray (2 chambers with a
central divider) or a midi tray (one large chamber). Press Save , then press
Continue .
4. Choose whether a quick rinse step is required in the protocol. A rinse will
dispense 10 mL of wash buer at the end of each step. If a rinse is desired,
highlight Enable on the screen. Press Continue .
Bandmate™Automated Western Processor
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Bandmate™Automated Western Blot Processor User Guide 7

5. On the last screen, input the times and reagent for each step in your protocol.
Buer wash steps can be repeated up to 7 times, but reagent steps cannot
be repeated. When selecting the step, the following may be chosen: Buer;
Reagent 1; Reagent 2; and End. Use the toggle button to highlight what needs
to be modified and use the knob to move through the selections. Selecting End
indicates that there are no more steps.
Below are the Preprogrammed Standard Western Blot Protocols, whereby
Step 1 already has the blocking solution in the tray and contains 2 reagent
(antibody addition) steps.
Table 4 Standard Mini
Step Reagent Repeat Fill Vol. Rock Time Rocks
Per Min.
Drain
To[1]
1Pre Fill 1X — 1 hr 15 Waste
21 1X — 1 hr 15 Waste
3Buer 3X 20 mL 10 mins 20 Waste
42 1X — 30 mins 15 Waste
5Buer 6X 20 mL 5 mins 20 —
[1] Change from Waste to Recover if antibodies need to be saved.
Table 5 Standard Midi
Step Reagent Repeat Fill Vol. Rock Time Rocks
Per Min.
Drain
To[1]
1Pre Fill 1X — 1 hr 15 Waste
21 1X — 1 hr 15 Waste
3Buer 3X 40 mL 10 mins 20 Waste
42 1X — 30 mins 15 Waste
5Buer 6X 40 mL 5 mins 20 —
[1] Change from Waste to Recover if antibodies need to be saved.
6. Choose the antibody recovery option if antibodies need to be recovered for
future use. Recovered reagents will be diverted to the tubes held in the brackets
on either side of the waste tray (position 1 for first antibody addition and position
2 for second antibody addition). If antibodies do not need to be recovered, all
reagent steps may be set to Waste. Press Save .
Note: If you choose to recover the antibody, place 50 mL recovery tubes, or
comparable 50 mL conical tubes, in the appropriate location in the waste tray.
Bandmate™Automated Western Processor
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8Bandmate™Automated Western Blot Processor User Guide

Run the processor
1. Turn the power switch on the back of the unit to the "on" position. The display
will illuminate, and the moving parts will return to their home positions. After a
few seconds, the home screen will display.
2. Program or select your preferred western blot protocol (see “Program the
processor” on page 7).
3. Fill the buer bottle in the back of the processor. Ensure that the hose hanging
from the cap reaches the bottom of the bottle. Leave the cap loose to allow air
into the bottle as liquid is pumped out.
For each wash, 10–75 mL of wash buer can be dispensed for mini gels and
10–150 mL for midi gels. We recommend using 20–30 mL per wash for mini gels
and 30–50 mL for midi gels. Ensure there is enough wash buer to complete the
protocol.
4. Flush the buer lines by selecting Flush from the home screen menu and
then pressing Accept . Press Fill until wash buer is dispensed from
all nozzles. This may take several seconds. The wash buer will dispense into
the waste tray.
5. If using primary and secondary antibodies, 2 reagent tubes will be required
for each blot being processed. Check to ensure the foil on the bottom of the
reagent tubes is not damaged. Screw one reagent tube firmly and completely
into the primary antibody position, noted with the number 1 toward the back of
the slider, and the other into the secondary antibody position, noted with the
number 2, just behind the buer dispensing nozzle.
6. Pipet the appropriate antibody solution into each reagent tube.
A standard-sized mini blot processed in a mini tray requires a minimum of
3.5 mL of each primary antibody and secondary antibody solutions. A standard-
sized midi blot processed in a midi tray requires a minimum of 7.5 mL of each
primary antibody and secondary antibody solutions.
7. Place blot(s) into the sample tray protein side up with protein lanes
perpendicular to the tray gates. If using a mini tray, which has a central divider,
make sure the blot is on the same side of the divider as the appropriate antibody
reagent tubes.
8. Pour blocking buer of choice in the tray. Use no more than 45 mL of blocking
buer per mini tray and no more than 90 mL blocking buer for midi trays.
Replace the tray cover and make sure it is fully seated.
Tray Size Minimum Volume Maximum Volume
Mini 3.5 mL 45 mL
Midi 7.5 mL 90 mL
Bandmate™Automated Western Processor
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Bandmate™Automated Western Blot Processor User Guide 9

9. To recover the antibody solutions, place empty 50 mL uncapped conical tubes
in the appropriate spaces on each side of the waste tray (space #1 for the
primary antibody and #2 for the secondary antibodies).
Note: For antibody recovery from midi trays, conical tubes must be added on
both sides of the waste tank as recovered solutions will be distributed in both
tubes.
10. Check the waste tank to make sure that it is empty. Ensure that the funnel tray
and waste trays are pushed all the way to the back and are in place. Close the
waste compartment and the work area window.
11. Select the desired protocol from the menu and press Run .
12. Select the trays to be used for the protocol. Follow instructions on the screen to
indicate which section(s) are in use.
13. Press Continue .
14. Press Run .
15. Once the run is complete, press Home to stop the rocker and return to the
home screen. Open the window and remove the blot. The blot is now ready
for downstream processing (chemiluminescent or colorimetric detection) and/or
immediate imaging (fluorescence).
Clean the processor
1. Wash the sample tray and tray cover using a gentle laboratory detergent.
2. Remove the Reagent Tubes from the instrument.
3. If the processor is not going to be used again for some time, then flush the
buer line with distilled water to prevent salt build-up. To flush, place the wash
buer hose into a container of distilled water and use Flush to run water through
the line and into the tray.
General maintenance
Replacing buers
When replacing or adding additional wash buer to the wash bottle, it is
recommended to flush the lines to ensure no bubbles were introduced when the
buer was replenished.
Bandmate™Automated Western Processor
Operate the Bandmate™Automated Western Blot Processor
10 Bandmate™Automated Western Blot Processor User Guide

Calibration
The pumps that deliver wash buer to the tray have been calibrated at the factory.
To check or re-calibrate the pumps, use the Calibration function. Pumps should be
re-calibrated every 6 months.
1. From the home screen, select Maintenance. On-screen instructions may be
used as a guide for the calibration process. A 50 mL graduated cylinder is
recommended to perform the calibration.
Note: Prior to calibration of the pumps, confirm that the wash buer line is
completely full of liquid. If there are bubbles in the line, use the Flush function to
remove them.
2. Open the waste compartment and remove the funnel tray. If using a larger
graduated cylinder and extra room is needed, remove the waste tank.
Alternatively, detach the hose from the hose nozzle and place it in the graduated
cylinder.
Note: Be careful not to stretch or distort the hose.
3. Place the graduated cylinder under the buer, position as indicated on the
graphical display, and press Fill to dispense liquid into the cylinder. The
pump will dispense approximately 40 mL.
4. Once the pump has stopped running, measure the actual amount of liquid
dispensed and enter the value using the encoder knob. Measure the volume
from the other positions in the same way and record the value. Press Save .
The pumps are now calibrated.
5. Replace the waste tank and funnel tray and close the waste compartment.
The amount of liquid dispensed by the pumps is temperature-dependent. This is
accounted for in the factory calibration.
The Bandmate™processor calibrates based on temperature. "Warm" calibration is
indicated by the red numbers, while "cold" calibration is indicated by the blue
numbers. These numbers represent the pump coecients, ranging from 100 to 300
with 200 being the intrinsic value of the pump. The cuto temperature between
"warm" and "cold" is 12°C. To check the calibration, and to use the processor at
Bandmate™Automated Western Processor
Operate the Bandmate™Automated Western Blot Processor
Bandmate™Automated Western Blot Processor User Guide 11

dierent temperatures, perform a "warm" and a "cold" calibration at the warmest and
coldest temperatures at which the processor is planned to be used. If the processor
is only used at one temperature, it is unnecessary to calibrate it at any other
temperature.
General system cleaning
•Rinse the waste tank and funnel tray. The trays are resistant to chemicals
typically used in a western blot, but some of the components of blocking buers
(nonfat dry milk, BSA, etc.) can form deposits on the surface.
•If needed, use water and a lab tissue to clean the display screen protector, work
area window, and any interior surfaces of the instrument. Using alcohol is not
recommended as it may fog plastic surfaces over time.
Instrument dimensions and specifications
Dimensions 55 cm × 39 cm × 43 cm
Weight 15 kg (32 lbs)
Capacity 4 mini blots, 2 midi blots
Rocks per minute 5-40 rpm
Maximum washes per step 10
Minimum reagent per blot 3.5 mL (mini), 7.5 mL (midi)
Maximum number of protocol steps 9
Maximum number of protocols 7
Buer dispensing volume 10-150 mL
Incubation step time range 0.1 sec to 96 hrs
Power requirements 12 VDC, 2.1 A
Relative humidity 5-90%
Operating temperature 4-40°C
Altitude 2,000 m
Storage temperature -40 to 50°C
Bandmate™Automated Western Processor
Operate the Bandmate™Automated Western Blot Processor
12 Bandmate™Automated Western Blot Processor User Guide

Troubleshooting
Observation Possible cause Recommended action
Processor won't start. Power cord plug is inserted in
an inactive wall outlet and/or not
fully inserted in the socket on
the back of the processor.
Ensure the power cord plug is inserted in a live
wall outlet and/or fully inserted in the socket on
the back of the processor.
Processor stopped working. Processor electronics errored. Turn the system o for 1 minute to allow
the electronics to reset. If the processor does
not turn on after resetting, contact technical
support.
Processor loses power. Processor was unplugged or a
power outage occurred.
Restore power to the unit when possible. If a
run was in progress, it will automatically re-start
at the point it had reached when power was
lost. No other special measures are needed. If
you wish to terminate the recovered run, press
the "home" button when the program recovery
screen is displayed..
Processor stops during a run
and displays an error message.
An error was detected. The run will resume once the error is corrected.
The most common error is that the work area
window may not be fully closed.
Antibodies are not dispensed. The points on the sample tray
are not facing toward the back
of the instrument or have been
damaged..
Ensure that the points on the sample tray
are facing toward the back of the instrument,
and that they are still sharp. The processor
ships with several trays, but replacement trays
are available for purchase if points become
damaged.
Buer is not dispensed during
the run.
The end of the buer hose may
not have been resting at the
bottom of the filled buer bottle,
or the cap may be tight and not
allowing air flow into the bottle.
Ensure that the end of the buer hose is resting
in the bottom of a filled bottle of buer. The
buer bottle cap should be slightly loose in order
to allow air to flow into the bottle..
Limited product warranty
Life Technologies Corporation and/or its aliate(s) warrant their products as
set forth in the Life Technologies' General Terms and Conditions of Sale
at www.thermofisher.com/us/en/home/global/terms-and-conditions.html. If you
have any questions, please contact Life Technologies at www.thermofisher.com/
support.
Bandmate™Automated Western Processor
Troubleshooting
Bandmate™Automated Western Blot Processor User Guide 13

Safety
WARNING! GENERAL SAFETY. Using this product in a manner not specified in
the user documentation may result in personal injury or damage to the instrument
or device. Ensure that anyone using this product has received instructions in
general safety practices for laboratories and the safety information provided in this
document.
·Before using an instrument or device, read and understand the safety information
provided in the user documentation provided by the manufacturer of the
instrument or device.
·Before handling chemicals, read and understand all applicable Safety Data Sheets
(SDSs) and use appropriate personal protective equipment (gloves, gowns, eye
protection, and so on). To obtain SDSs, see the “Documentation and Support”
section in this document.
Symbols on this instrument
Symbols may be found on the instrument to warn against potential hazards or
convey important safety information. In this document, the hazard symbol is used
along with one of the following user attention words.
•CAUTION!—Indicates a potentially hazardous situation that, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices.
•WARNING!—Indicates a potentially hazardous situation that, if not avoided,
could result in death or serious injury.
•DANGER!—Indicates an imminently hazardous situation that, if not avoided, will
result in death or serious injury.
14 Bandmate™Automated Western Blot Processor User Guide

Standard safety symbols
Symbol and description
CAUTION! Risk of danger. Consult the manual for further safety information.
CAUTION! Risk of electrical shock.
Symbole et description
MISE EN GARDE ! Risque de danger. Consulter le manuel pour d’autres
renseignements de sécurité.
MISE EN GARDE ! Risque de choc électrique.
Safety
Symbols on this instrument
Bandmate™Automated Western Blot Processor User Guide 15

Control and connection symbols
Symbols and
descriptions
On (Power)
O (Power)
Earth (ground) terminal
Protective conductor terminal (main ground)
Direct current
Alternating current
Both direct and alternating current
Conformity symbols
Conformity mark Description
Indicates conformity with safety requirements for Canada and U.S.A.
Indicates conformity with China RoHS requirements.
Indicates conformity with European Union requirements.
Indicates conformity with Australian standards for electromagnetic
compatibility.
Indicates conformity with the WEEE Directive 2012/19/EU.
CAUTION! To minimize negative environmental impact from
disposal of electronic waste, do not dispose of electronic
waste in unsorted municipal waste. Follow local municipal
waste ordinances for proper disposal provision and contact
customer service for information about responsible disposal
options.
Safety
Symbols on this instrument
16 Bandmate™Automated Western Blot Processor User Guide

Safety information for instruments not manufactured by Thermo
Fisher Scientific
Some of the accessories provided as part of the instrument system are not designed
or built by Thermo Fisher Scientific. Consult the manufacturer's documentation for
the information needed for the safe use of these products.
Instrument safety
General
CAUTION! Do not remove instrument protective covers. If you remove the
protective instrument panels or disable interlock devices, you may be exposed to
serious hazards including, but not limited to, severe electrical shock, laser exposure,
crushing, or chemical exposure.
CAUTION! Solvents and Pressurized fluids. Wear eye protection when working
with any pressurized fluids. Use caution when working with any polymeric tubing that
is under pressure:
·Extinguish any nearby flames if you use flammable solvents.
·Do not use polymeric tubing that has been severely stressed or kinked.
·Do not use polymeric tubing with tetrahydrofuran or nitric and sulfuric acids.
·Be aware that methylene chloride and dimethyl sulfoxide cause polymeric tubing
to swell and greatly reduce the rupture pressure of the tubing.
·Be aware that high solvent flow rates (~40mL/min) may cause a static charge to
build up on the surface of the tubing and electrical sparks may result.
Safety
Safety information for instruments not manufactured by Thermo Fisher Scientific
Bandmate™Automated Western Blot Processor User Guide 17

Physical injury
CAUTION! Moving and Lifting Injury. The instrument is to be moved and
positioned only by the personnel or vendor specified in the applicable site
preparation guide. Improper lifting can cause painful and permanent back injury.
Things to consider before lifting or moving the instrument or accessories:
·Depending on the weight, moving or lifting may require two or more persons.
·If you decide to lift or move the instrument after it has been installed, do not
attempt to do so without the assistance of others, the use of appropriate moving
equipment, and proper lifting techniques.
·Ensure you have a secure, comfortable grip on the instrument or accessory.
·Make sure that the path from where the object is to where it is being moved is
clear of obstructions.
·Do not lift an object and twist your torso at the same time. Keep your spine in a
good neutral position while lifting with your legs.
·Participants should coordinate lift and move intentions with each other before
lifting and carrying.
·For smaller packages, rather than lifting the object from the packing box, carefully
tilt the box on its side and hold it stationary while someone else slides the contents
out of the box.
Safety
Instrument safety
18 Bandmate™Automated Western Blot Processor User Guide

Electrical safety
WARNING! Do not overfill trays with liquid. Excess liquid can overflow into the
control unit and possibly cause electrical shock. Follow the appropriate instructions
for reagent amounts and empty any remaining liquid in the waste containers upon run
completion.
AVERTISSEMENT ! Ne pas trop remplir les plateaux de liquide. Le liquide
excédentaire peut déborder dans l’unité de commande et provoquer possiblement
une décharge électrique. Suivre les instructions appropriées pour les quantités
de réactif et vider tout liquide restant dans les conteneurs de déchets une fois
l’opération terminée.
WARNING! Use outside of the workflows described in this manual may put the
operator at risk of dangerous exposure to electrical shock. Do not use this instrument
for any purposes or in any configurations not described in this manual.
AVERTISSEMENT ! L’utilisation en dehors des flux de travail décrits dans le présent
manuel assujettit l’opérateur à un risque d’exposition dangereuse à une décharge
électrique. Ne pas utiliser cet instrument à des fins ou dans des configurations qui ne
sont pas décrites dans le présent manuel.
WARNING! Ensure appropriate electrical supply. For safe operation of the
instrument:
·Plug the system into a properly grounded receptacle with adequate current
capacity.
·Ensure the electrical supply is of suitable voltage.
·Never operate the instrument with the ground disconnected. Grounding continuity
is required for safe operation of the instrument.
AVERTISSEMENT ! Veiller à utiliser une alimentation électrique appropriée.
Pour garantir le fonctionnement de l’instrument en toute sécurité :
·Brancher le système sur une prise électrique correctement mise à la terre et de
puissance adéquate.
·S’assurer que la tension électrique est convenable.
·Ne jamais utiliser l’instrument alors que le dispositif de mise à la terre
est déconnecté. La continuité de la mise à la terre est impérative pour le
fonctionnement de l’instrument en toute sécurité.
WARNING! Power Supply Line Cords. Use properly configured and approved line
cords for the power supply in your facility.
Safety
Instrument safety
Bandmate™Automated Western Blot Processor User Guide 19

AVERTISSEMENT ! Cordons d’alimentation électrique. Utiliser des cordons
d’alimentation adaptés et approuvés pour raccorder l’instrument au circuit électrique
du site.
WARNING! Disconnecting Power. To fully disconnect power either detach or
unplug the power cord, positioning the instrument such that the power cord is
accessible.
AVERTISSEMENT ! Déconnecter l’alimentation. Pour déconnecter entièrement
l’alimentation, détacher ou débrancher le cordon d’alimentation. Placer l’instrument
de manière à ce que le cordon d’alimentation soit accessible.
Cleaning and decontamination
CAUTION! Cleaning and Decontamination. Use only the cleaning and
decontamination methods that are specified in the manufacturer user documentation.
It is the responsibility of the operator (or other responsible person) to ensure that the
following requirements are met:
·No decontamination or cleaning agents are used that can react with parts of the
equipment or with material that is contained in the equipment. Use of such agents
could cause a HAZARD condition.
·The instrument is properly decontaminated a) if hazardous material is spilled onto
or into the equipment, and/or b) before the instrument is serviced at your facility or
is sent for repair, maintenance, trade-in, disposal, or termination of a loan. Request
decontamination forms from customer service.
·Before using any cleaning or decontamination methods (except methods that
are recommended by the manufacturer), confirm with the manufacturer that the
proposed method will not damage the equipment.
MISE EN GARDE ! Nettoyage et décontamination. Utiliser uniquement les
méthodes de nettoyage et de décontamination indiquées dans la documentation du
fabricant destinée aux utilisateurs. L’opérateur (ou toute autre personne responsable)
est tenu d’assurer le respect des exigences suivantes:
·Ne pas utiliser d’agents de nettoyage ou de décontamination susceptibles de
réagir avec certaines parties de l’appareil ou avec les matières qu’il contient et de
constituer, de ce fait, un DANGER.
·L’instrument doit être correctement décontaminé a) si des substances
dangereuses sont renversées sur ou à l’intérieur de l’équipement, et/ou b) avant de
le faire réviser sur site ou de l’envoyer à des fins de réparation, de maintenance,
de revente, d’élimination ou à l’expiration d’une période de prêt (des informations
sur les formes de décontamination peuvent être demandées auprès du Service
clientèle).
·Avant d’utiliser une méthode de nettoyage ou de décontamination (autre que
celles recommandées par le fabricant), les utilisateurs doivent vérifier auprès de
celui-ci qu’elle ne risque pas d’endommager l’appareil.
Safety
Instrument safety
20 Bandmate™Automated Western Blot Processor User Guide