Invitrogen EVOS XL User manual

For Research Use Only.Not for use in diagnostic procedures.
EVOS™XL Imaging System
For Transmitted Light Applications
Catalog Number AME3300
Publication Number MAN0007987
Revision A.0

Information in this document is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTIONWITH OR ARISING FROM
THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history:MAN0007987
Revision
Date
Description
A.0
09 October 2017
Update branding,legal/regulatorylanguage, reorganizecontent.
1.0
8 April 2013
Basis for this revision.
Important Licensing Information: These productsmay be covered by one or more LimitedUse Label Licenses. By use of these
products, you accept the terms and conditions ofall applicable Limited Use Label Licenses.
Manufacturer: Life TechnologiesCorporation | 2202520th AveSE St #100 | Bothell, WA 98021
Trademarks: Alltrademarks are the property ofThermo Fisher Scientific and itssubsidiaries unless otherwise specified.Kimwipesis
a registered trademarkofKimberly-Clark Corporation.
© 2017 Thermo Fisher Scientific Inc. All rights reserved.

EVOS™XL Imaging System User Guide 1
Contents
About this guide......................................................................................................................... 3
1. Product information ...........................................................................................................4
Product description ..........................................................................................................................................................4
Standard items included ................................................................................................................................................4
Instrument exterior components and mechanical controls ................................................................................5
Onscreen controls .............................................................................................................................................................6
2. Installation ........................................................................................................................... 7
Operating environment ..................................................................................................................................................7
Transport the EVOS™XL Imaging System to the installation site ...................................................................7
Unlock mechanical stage................................................................................................................................................8
Connect p ower supply....................................................................................................................................................8
USB and DVI output ports ............................................................................................................................................8
Condenser shield ..............................................................................................................................................................9
Install the EVOS™XL Imaging System in a cell culture hood..........................................................................10
3. Basic operation..................................................................................................................11
4. Advanced operation ..........................................................................................................14
Manage users ...................................................................................................................................................................14
Save i mages and manage fil es ....................................................................................................................................16
Use the QuickSave option to save multiple images ............................................................................................18
Record time lapse images ............................................................................................................................................20
Count cells.........................................................................................................................................................................23
Review images .................................................................................................................................................................24
Use digital zoom .............................................................................................................................................................25
Connect the EVOS™XL Imaging System to a network ......................................................................................28
Update the software.......................................................................................................................................................31
5. Care and maintenance .....................................................................................................33
General care ......................................................................................................................................................................33
Objec tive lens care ..........................................................................................................................................................33
Stage care ...........................................................................................................................................................................33
Decontamination procedures .....................................................................................................................................34

2
EVOS™XL Imaging System User Guide
Appendix A: Troubleshooting................................................................................................35
Image quality issues.......................................................................................................................................................35
Software interface issues ..............................................................................................................................................36
Mechanical issues...........................................................................................................................................................36
Appendix B: System specifications ......................................................................................37
Appendix C: Controls glossary..............................................................................................38
On screen controls ..........................................................................................................................................................38
Mechanical controls .......................................................................................................................................................42
Appendix D: Safety..................................................................................................................44
Safety conventions used in this document.............................................................................................................45
Symbols on instruments ...............................................................................................................................................46
Safety labels on instruments .......................................................................................................................................48
General instrument safety............................................................................................................................................49
Chemical safety ...............................................................................................................................................................50
Chemical waste safety...................................................................................................................................................51
Electrical safety................................................................................................................................................................52
Physical hazard safety...................................................................................................................................................53
Biological hazard safety................................................................................................................................................53
Safety and electromagnetic compatibility (EMC) standards............................................................................54
Documentation and support..................................................................................................55
Obtaining support ..........................................................................................................................................................55

EVOS™XL Imaging System User Guide 3
About this guide
Audience
This user guide is for laboratory staff operating, maintaining, and analyzingdata
using the EVOS™XL Imaging System.
User attention
words
Two user attention words appear in Thermo Fisher Scientific user documentation.
Each word implies a particular level of observation or action as described below.
Note: Provides information that may be of interest or help but is not critical
to the use of the product.
IMPORTANT! Provides information that is necessary for proper instrument
operation, accurate installation, or safe use of a chemical.
Safety alert words
Four safety alert words appear in Thermo Fisher Scientific user documentation at
points in the document where you need to be aware of relevant hazards. Each
alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a particular
level of observation or action, as defined below:
IMPORTANT! – Provides information that is necessary for proper
instrument operation, accurate installation,or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher
Scientific document appears with an open triangle figure that contains a hazard
symbol. These hazard symbols are identical to the hazard symbols that are affixed
to Thermo Fisher Scientific instruments (see “Safety symbols”, page 47).
SDSs
The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher
Scientific are available to you free 24 hours a day. For instructions on obtaining
SDSs, see “Safety Data Sheets (SDS)”, page 55.
IMPORTANT! For the SDSs of chemicals not distributed by Thermo Fisher
Scientific contact the chemical manufacturer.

4
EVOS™XL Imaging System User Guide
1. Product information
Product description
EVOS™XL Imaging
System
The Invitrogen™EVOS™XL Imaging System (Cat. No. AME3300) is a fully
automated, digital, inverted imaging system. The system is designed for a broad
range of transmitted light applications including time-lapse imaging, manual-
assist cell counting, and image review.
Product use
For Research Use Only. Not for use in diagnostic procedures.
Standard items included
Before setting up the EVOS™XL Imaging System, unpack the unit and accessories
and verify all parts are present. Contact your distributor if anything is missing.
Damage claims must be filed with the carrier; the warranty does not cover in-
transit damage.
Note: If you do not have your distributor information, contact Technical
Support (page 55).
•EVOS™XL Imaging System, per order
•Power adapter and power cord
•Dust cover
•USB flash drive (includes User Guide and Quick Start Guide)
•Condenser shield, removable
•Accessories box
•USB mouse
•Stage lock pin (in place on stage)
•
Condenser slider: Diffuser
IMPORTANT! Wiping the computer supplied with the EVOS™XL Imaging
System (i.e., erasing the hard drive to remove all programs, files, and the
operating system) voids the product warranty. Do not install third party
software or update the operating system.

EVOS™XL Imaging System User Guide 5
Instrument exterior components and mechanical controls
Power switch Objective selection wheel
Power input jack Focus knobs
USB and DVI ports Stage Y-axis knob
Coarse stage positioning knobs Phase annuli selector
Stage X-axis knob Condenser slider slot

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EVOS™XL Imaging System User Guide
Onscreen controls
Login button Scale bar and toolbar options
Light ON/OFF button Settings control button
Illumination slider Save image button
Capture image button QuickSave option
Color adjustment button Selected objective
Note: See “Appendix C: Controls glossary” on page 38 for more details
about onscreen and mechanical controls.

EVOS™XL Imaging System User Guide 7
2. Installation
Operating environment
•Place the EVOS™XL Imaging System on a level surface away from vibrations
from other pieces of equipment.
•Allow at least 5 cm (2 in) free space at the back of the instrument to allow for
proper ventilation and prevent overheating of electronic components.
•Set up the EVOS™XL Imaging System away from direct light sources, such as
windows. Ambient room lighting can enter the imaging path and affect the
image.
•Operating temperature range: 4°–32°C (40°–90°F).
•Relative humidity range: 30–90%.
IMPORTANT! EVOS™XL Imaging System should not be subjected to UV
sterilization. UV degrades many materials, including plastic. Damage from
UV exposure is not covered under the manufacturer’s warranty.
Transport the EVOS™XL Imaging System to the installation site
1. IMPORTANT! ALWAYS lock stage with the stage lock pin before moving
the instrument.
2. Lift the instrument by grasping it firmly with both hands under the support
arm , balancing the weight as shown in the image below.
3. To transport the EVOS™XL Imaging System to a different facility, use the
original packaging materials if possible. Always be sure the instrument is
properly cushioned and braced to prevent damage.
IMPORTANT! Never allow the EVOS™
XL Imaging System to be subjected to
sudden impact or excessive vibration. Handle the instrument with care to
prevent damage.

8
EVOS™XL Imaging System User Guide
Unlock mechanical stage
Remove stage lock
pin
Before using the mechanical stage for the
first time, remove the stage lock pin
from the back right-hand corner of the
stage plate.
Pull firmly to remove this pin.
Note: Always secure the mechanical stage with the stage lock pin before
moving the instrument. Store the stage lock pin in your accessories box for
future use.
Connect power supply
Connect power
adapter
1. Turn the power switch to the “” (OFF)
position before connecting the power adapter.
2. Connect the power adapter to the power jack
on the right side of the instrument base, attach
the cord to the adapter, and plug the cord into
an outlet.
IMPORTANT! Always use the correct power supply. The power adapter
specifications appear on the serial number label (front of LCD hinge) and
in “Appendix B: Specifications”, page 37. Damage due to an incompatible
power adapter is not covered by warranty.
USB and DVI output ports
USB ports
Plug the mouse and the USB flash drive into
the USB ports located on the bottom right
of the support arm. You may also plug in a
USB keyboard (not included) for text input.
A DVI port allows output to a projector or
other display (DVI cable not included). This
port produces digital output only; EVOS™XL
Imaging System is compatible with either a
DVI-D or DVI-I display.
DVI port