Ismatec MasterFlex Reglo ICC User manual

Reglo ICC
Operating Manual

2 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
Reglo ICC Operating Manual
Download Pump Control Software:
www.masterflex.com

3 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
13. Tubing Size and Flow Rate Chart ......................................16
14. Reglo ICC Serial Command Protocol ...............................17
14.1 Overview ..................................................................................17
14.2 Physical Layer ...........................................................................17
14.3 Message Format......................................................................17
14.4 Addressing................................................................................17
14.5 Definitions.................................................................................18
14.6 Data Type Formats ..................................................................18
14.6.1 Boolean..............................................................18
14.6.2 Direction ............................................................18
14.6.3 Discrete Type 1..................................................18
14.6.4 Discrete Type 2..................................................18
14.6.5 Discrete Type 3..................................................18
14.6.6 Discrete Type 4..................................................19
14.6.7 Discrete Type 5..................................................19
14.6.8 Discrete Type 6..................................................19
14.6.9 Fractional Type 1...............................................19
14.6.10 Volume Type 1................................................19
14.6.11 Time Type 1 .....................................................19
14.6.12 Time Type 2 .....................................................19
14.6.13 String................................................................19
14.6.14 Language.........................................................20
15. Response Messages...........................................................20
15.1 Status Response ......................................................................20
15.2 Data Response.........................................................................20
16. Request Messages .............................................................21
16.1 Set Pump Address...................................................................21
16.2 Device Command ...................................................................21
16.3 Tube Diameter Index ..............................................................25
Table of Contents
1. Safety Precautions ................................................................5
2. Warranty ................................................................................5
3. Product ..................................................................................6
4. Rear Panel .............................................................................6
5. Main Voltage.........................................................................6
6. Pump Operation...................................................................7
6.1 Key Pad and Screen ..................................................................7
6.2 Icons.............................................................................................7
6.3 Changing Parameters...............................................................8
6.4 Menus..........................................................................................8
6.4.1 STATUS Menu .....................................................8
6.4.2 PUMPING Menu .................................................9
6.4.3 SETUP Menu .......................................................11
6.4.4 CALIBRATION Menu ..........................................11
6.4.5 GLOBAL SETTINGS Menu.................................12
6.5 Installing Tubing.........................................................................12
6.6 Information Screens ..................................................................13
6.7 Differential Pressure...................................................................13
6.8 When the Pump is Idle..............................................................13
7. Over Current Protection ......................................................13
8. Cassettes ...............................................................................14
8.1 Ordering Spare Parts.................................................................14
8.2 Cassette Material.......................................................................14
8.3 Pressure Lever Cassettes..........................................................14
9. Repair ....................................................................................14
10. Cleaning ..............................................................................14
11. Disposal...............................................................................14
12. Technical Specifications......................................................15

4 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
18.6 System 31
18.6.1 Get pump firmware version 31
8.6.2 Set pump’s temporary display name 31
18.6.3 Serial number 31
8.6.4 Language 31
18.6.5 Channel count 32
18.6.6 Roller count 32
18.6.7 Total Revolutions 32
18.6.8 Volume total 32
18.6.9 Time total 32
18.7 Events 33
18.7.1 Channel stop 33
18.7.2 Channel status update 33
19. Regulatory Information 34
19.1 Manufacturing Information 34
19.2 CE Conformity 34
19.3 International Standards 34
19.4 CE Marketing 35
19.5 Connected Equipment 35
17. Event Messages 25
17.1 Channel Stop 26
17.2 Channel Status update 26
18. Examples 27
18.1 Communication Management 27
18.1.1 Get serial protocol version 27
18.1.2 Event message enable 27
18.2 Pump Drive 27
18.2.1 Start pumping 27
18.2.2 Stop pumping 27
18.2.3 Get direction 27
18.2.4 Set direction 28
18.3 Modes and Settings 28
18.3.1 Operational pumping mode 28
18.3.2 Pumping rate (drive speed) 28
18.3.3 Pumping rate (volume/time) 28
18.3.4 Volume 28
18.3.5 Run time 29
18.3.6 Pause time 29
18.3.7 Cycle Count 29
18.4 Configuration 29
18.4.1 Tubing Inside Diameter 29
18.4.2 Backsteps 29
18.5 Calibration 30
18.5.1 Volume (target) 30
18.5.2 Volume (measured) 30
18.5.3 Time 30
18.5.4 Get time since last calibration 30
18.5.5 Start calibration 30
18.5.6 Cancel calibration in progress 31 We recommend that you read this operating manual
carefully. When operating a pump, certain hazards
cannot be excluded. MASTERFLEX is not liable for any
damage resulting from the use of an ISMATEC®pump.
MASTERFLEX is not responsible for the unsafe handling
of chemicals.

5 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
1. Safety Precautions
ISMATEC ® tubing pumps are designed for pumping and dispensing applications in
laboratories and industry. As such it is assumed that Good Laboratory Practice (GLP) and
the following recommendations will be observed.
The pump must not be operated outside the designed operating and environmental
conditions. In particular, it is the user’s responsibility not to exceed the specified
maximum differential pressure for the pump head.
The pump must not be used:
For any medical applications.
In explosion proof chambers or in the presence of flammable gases or fumes.
The pump must be switched OFF when pump heads, cassettes or tubing are
inserted or changed.
The permeability of tubing depends on the material used and pressure conditions.
Tubing can also become electro-statically charged. Please be aware of possible hazards
when handling tubing in explosion-proof chambers.
Pump heads consist of rotating parts. Therefore, the pump must not be operated
before the cassettes are fully seated on the pump head.
Do not manipulate the pump head before the pump is switched off and disconnected
from the mains power supply.
Use extreme caution to ensure that no part of your body such as fingers, long hair,
jewelry, or loose objects such as cables and tubing, etc. are near the pump head
during operation.
Tubing can tear and burst during operation. The necessary safety measures based on
the specific situation must be taken. When pumping corrosive media we recommend
placing the pump in a collecting basin.
The pump should not come in contact with liquid.
Do not open or remove the housing while the pump is operating.
Service and repairs carried out by the customer or by third-party companies voids
all warranties.
Appliance coupler of the power supply is used to disconnect the device from mains supply.
2. Warranty
We warrant the pump to be free of defects, provided they have been installed and operated
correctly according to our operating instructions for a period of 2 years from date of purchase.
If production or material faults can be proven, the defective parts will be repaired or replaced
free of charge at our discretion. A defective pump must be returned in the original ISMATECe
packaging or in packaging of equal quality. The duration of the warranty is not affected by
making a claim for warranty service. Further claims are excluded. Shipping costs are charged
to the customer.
Our warranty becomes invalid in the case of:
Improper operation by the user, or if the pump is diverted from its proper use.
Unauthorized modification or misuse by the user or by a third party.
Improper site preparation and maintenance.
Operation outside of the environmental and electrical specifications for the product.
Use of third-party software, hardware, accessories, or consumables purchased by the user
and which do not comply with our specifications.
Pinch Point Warning:
Do not place
your fingers on
or around the
pump head.
General Warning Caution:
Risk of
Electric Shock
Protective Earth:
(Ground)

6 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
6
3. Product
Reglo ICC is a peristaltic pump with independently controllable channels. The intended
use for this pump is precise fluid dispensing at low flow rates up to 43 mL/min for fluid
delivery applications.
Contents of the package:
Reglo ICC Pump (type as ordered)
1 power supply
Cassettes
1 power cord with country specific plug
Operating Manual
Please check the package and its contents for transport damage. If you find any signs of
damage, please contact your local ISMATEC®representative immediately.
4. Rear Panel
1. 24 V DC power Inlet plug
2. On/Off Switch, circuit breaker
3. RS-232 In, DB9-female
4. RS-232 Out, DB9-male
5. USB 2.0, Mini-B
5.
1.
3.
4.
2.
5. Main Voltage
Use only the supplied power supply and cord for your Reglo ICC pump. DO NOT use any
other substitutes or damage may occur! The power supply is designed to provide DC
voltage for your Reglo ICC pump. It has built in circuit over current protection in the event
the pump is overloaded or a malfunction occurs. The power supply is connected to the
pump by a 4-pin power DIN snap and lock plug. Appliance coupler of the power supply is
used to disconnect the device from the mains supply.
Voltage 100–240VAC
50/60 Hz

7 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
7
6. Pump Operation
6.1 Key Pad and Screen
1. Run/Stop Button
a. Starts and stops the pump
b. Interrupts and resumes a dispensing cycle
2. Reset Button
a. Escape out of editing a parameter without changing
b. Resets dispensing cycle when pump is paused
3. Directional Arrows
a. Used to navigate screen and highlight selections
b. Up, down, left, right
4. OK/Enter Button
a. Press to edit parameter
b. Saves edit to parameter once complete
5. LCD Display
5.
4.
2.
3.
1.
6.2 Icons
1. 2. 3. 4. 5. 6.
7.
1. STATUS Menu
2. PUMPING Menu
3. SETUP Menu
4. CALIBRATION Menu
5. GLOBAL SETTINGS Menu
6. Channel Number/Next Screen
7. Channel Rotation Direction

8 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
8
6.3 Changing Parameters
Changing parameters is done by moving the cursor around the LCD screen
using the four directional arrows. The arrows are used to navigate the menus
and change numeric values and pumping modes. Using the arrows, the cursor
can be moved to any field to select or edit.
Once the cursor is in the field that you would like to select or edit, press the
OK/Enter button. When highlighting numeric values, the cursor will highlight
the entire value. Pressing OK/Enter will then allow the cursor to highlight only
one digit for editing. Using the directional arrows up or down will increase or
decrease the value and left and right will move the cursor to the next digit to
be edited. Once the value is correct, press OK/Enter and the cursor will then highlight the
entire value exiting the edit mode. When highlighting pumping modes, tubing ID, and
language, the OK/Enter button will allow the parameter to be changed by toggling through a
list using the directional arrows. Once the selection has been made, pressing the OK/Enter
button will save the selection and exit the editing mode.
The Run/Stop button is used to start or stop all the channels. When the pump is
idle, pressing the Run/Stop button will start pumping to the settings for each
channel. When the pump is running, pressing the Run/Stop button will stop all
channels. If a timed or repeating pumping mode is being used, pressing the
Run/Stop button will pause the program. Pressing the Run/Stop button again
will resume the program.
The Reset button is used to cancel editing or reset pumping modes. When a
field has been selected for editing, pressing the Reset button will exit the
editing mode and restore the original value. Also, when the pump is paused,
pressing the Reset button will reset all the pumping modes back to their start
point (number of cycles, time, volumes, etc.).
6.4 Menus
6.4.1 STATUS Menu
The STATUS Menu has three screens. The Status screen (a) displays the current pumping
parameters for each channel. The unit displayed varies depending on the pumping mode
selected. The Total Volume screen (b) displays the total volume that has been pumped since the
last time it was reset. The Total Time screen (c) displays the total hours pumped for each channel
since the last time it was reset. The time and volume can be reset by navigating to the desired
value and pressing OK/Enter button. Highlighting the arrow in the upper right hand corner of the
screen and pressing the OK/Enter button will toggle through the three status screens.
a. b. c.

9 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
9
6.4.2 PUMPING Menu
The PUMPING Menu allows you to set up the pumping parameters for all of the available
channels. The channel number that is being programmed is indicated in the upper right hand
corner. You can select the rotation direction, pumping mode, and values for each channel in the
PUMPING menu.
6.4.2.1 Flow Rate Mode
Flow Rate Mode provides a continuous operation
at a set rate and direction. Adjustable parameters
are rotation direction, flow rate, and flow rate units.
Rotation direction can be selected as clockwise
(CW) or counter-clockwise (CCW). Flow rate can be
changed in the range specified by the selected unit
and/or tubing inner diameter (ID) (see Section
6.4.3). Selectable flow rate units are RPM, μL/min,
mL/min, and L/min. Flow rate and rotation direction
can be changed during the operation as well.
6.4.2.2 Volume Over Time Mode
Volume Over Time Mode allows the user to
dispense a desired volume over a desired time.
The pump will determine the speed of rotation by
set volume and time. Adjustable parameters are
rotation direction, volume, volume units, time, and
time units. Rotation direction can be selected as
CW or CCW. Combinations of time and volume
can be set to any value as long as it is in the range
of the pump’s capabilities. If the time value
entered is less than what the pump is capable of
for desired volume, it will default to the shortest
dispense time possible. Volume units selectable
are μL, mL and L. Time units selectable are seconds, minutes, and hours. None of the
parameters are changeable during pumping or when operation is paused. Dispensing program
can be reset during operation by pressing the Reset button after pumping is paused.
6.4.2.3 Volume Mode
Volume Mode allows the user to dispense a set
volume with a set flow rate. Adjustable parameters
are rotation direction, flow rate, flow rate units,
volume, and volume units. Rotation direction can
be selected as CW or CCW. Flow rate can be
changed in the range specified by the selected unit
and/or tubing ID (see Section 6.4.3). Selectable flow
rate units are RPM, μL/min, mL/min, and L/min.
Selectable volume units are μL, mL, and L. Only
flow rate can be changed during pumping or when
the operation is paused. Dispensing program can
be reset during operation by pressing the Reset
button after pumping is paused.

10 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
6.4.2.4 Time Mode
Time Mode allows the user to dispense for a set
time duration with a set flow rate. Adjustable
parameters are rotation direction, flow rate, flow
rate units, time, and time units. Rotation direction
can be selected as CW or CCW. Flow rate can be
changed in the range specified by the selected unit
and/or tubing ID (see Section 6.4.3). Selectable flow
rate units are RPM, μL/min, mL/min, and L/min.
Selectable time units are sec, min, and hours. Only
flow rate can be changed during pumping or when
the operation is paused. Dispensing program can
be reset during operation by pressing the Reset
button after pumping is paused.
6.4.2.5 Volume with Pause Mode
Volume with Pause Mode allows the user to
dispense a set volume over multiple cycles.
Adjustable parameters are rotation direction, flow
rate, flow rate units, volume, volume units, pause
time between cycles, pause time unit, and number
of cycles. Rotation direction can be selected as CW
or CCW. Flow rate can be changed in the range
specified by the selected unit and/or tubing ID (see
Section 6.4.3). Selectable flow rate units are RPM,
μL/min, mL/min, and L/min. Selectable volume
units are μL, mL, and L. Selectable pause time units
are sec, min, and hours. Only flow rate can be changed during pumping or when the operation
is paused. Dispensing program can be reset during operation by pressing the Reset button
after pumping is paused.
6.4.2.6 Time with Pause Mode
Time with Pause Mode allows the user to dispense
for a set time duration over multiple cycles.
Adjustable parameters are rotation direction, flow
rate, flow rate units, time, time units, pause time
between cycles, pause time unit, and number of
cycles. Rotation direction can be selected as CW or
CCW. Flow rate can be changed in the range
specified by the selected unit and/or tubing ID (see
Section 6.4.3). Selectable flow rate units are RPM,
μL/min, mL/min, and L/min. Selectable time units
are sec, min, and hours. Selectable pause time units
are sec, min, and hours. Only flow rate can be changed during pumping or when the operation
is paused. Dispensing program can be reset during operation by pressing the Reset button
after pumping is paused.
6.4.2.7 Disabled Mode
Disabled Mode disables the selected channel for
pumping. The channels that are not anticipated to
pump any fluid should be set to this mode.

11 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
6.4.3 SETUP Menu
In the SETUP Menu the tubing ID is set by selecting the size of tubing being used in the
highlighted channel selected in the upper right hand corner. The tubing ID is selected from
the drop down menu. The number of roller backsteps for drip free dispensing can also be set
between 0–100 (0 = Default Value).
6.4.4 CALIBRATION Menu
In the CALIBRATION Menu each channel can be calibrated independently. The channel being
calibrated is listed in the upper right hand corner.
The Reglo ICC allows each channel to be set up and calibrated. This allows for maximum
flexibility and optimum channel to channel precision. Prior to the calibration each channel must
be configured for tubing ID, the number of backsteps that are desired for drip free dispensing,
and calibration of that channel.
The Reglo ICC allows you to enter the volume of fluid to dispense for calibration, dispense
time for the specified volume, and pumping direction. Once you have entered those values,
dispense desired volume into a container. Measure the actual amount of the fluid by weight
or volume and enter that value on the last screen to adjust the pump for your application. The
calibration can be interrupted by pressing the Run/Stop button which will cancel the existing
progress. New uninterrupted calibration run will be required in order to calibrate the channel
properly. When adjusting the dispense time for calibration, if requested time is shorter than
the pump’s physical capabilities, the dispense time will automatically default to minimum
achievable dispense time.

12 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
You can complete the calibration by pressing OK/Enter button following your measurement
input. You will receive a message and checkmark once your calibration is successful.
You can cancel the calibration after the calibration run by pressing the Reset button. You will
receive the message displaying that the calibration has been canceled.
6.4.5 GLOBAL SETTINGS Menu
In the GLOBAL SETTINGS Menu the display language can be selected from English, French,
Spanish, and German. The pump can also be reset to the factory defaults at this menu.
6.5 Installing Tubing
1. Switch the pump OFF.
2. Remove the cassette by slightly pressing the fixing-tongue and lifting it simultaneously.
3. Insert the 2-stop or 3-stop color-coded tubing with one stopper into the cassette.
4. Let the tubing hang down (prevents it from twisting).
5. Insert the tubing with the second stopper at the other end of the cassette.
6. Reinsert the cassette into the roller-head.
7. Make sure each cassette is positioned and aligned well between
dedicated segments on the bars.
8. Turn the pump ON.
9. When the pump is idle, release all cassettes to maximize tubing life
(beware of siphoning or back flow).

13 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
6.6 Information Screens
Indicates that the pump is being remotely
controlled by a PC via USB connection.
Indicates that the internal temperature has
exceeded a safe threshold and pumping will stop
automatically to protect the pump. Turn the pump
power switch OFF and check your parameters,
differential pressure, and pump head for blockage.
The pump will not operate in an overheated
condition. Allow the pump to cool before turning
the power switch back ON.
6.7 Differential Pressure
The Reglo ICC can be used for continuous duty at a maximum differential pressure of 1.0 bar
(smaller tube sizes and/or lever type cassettes allow higher pressures).
6.8 When the Pump is Idle
When the pump is idle, we recommend releasing the tubing cassette from the pump head.
Releasing the cassette on the right side is sufficient. This helps to protect the tubing from
unnecessary strain and prolongs its service life. However, a siphoning effect can occur when
the tubing cassette is released from the pump head. Use caution to prevent the fluid from
flowing back to the reservoir.
7. Over Current Protection
The Reglo ICC features an external power supply that has overload protection as well as a
combined circuit breaker and On/Off switch on the pump. There are NO replaceable fuses or
service items inside the pump. This system is designed to disable power to the pump drive in
case of excessive current to prevent damage. When an overload condition occurs, the pump
will power down and it is recommend to immediately switch the pump OFF and unplug the
pump from the wall. Before the pump is re-started, it is most important to check the reason
for the overload (e.g. excess differential pressure etc.). Only when the cause of the overload
has been detected and the failure corrected may the pump be started again. If the condition
persists, contact an ISMATEC®technical support representative immediately.

14 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
8. Cassettes
Your Reglo ICC can use the MS/CA Click-n-Go®cassettes which accept “3-Stop” tubing.
See Page 16 for more information. When using new tubing for the first time, depending on
the tubing used (hardness and diameter), the pump may not be primed and can not deliver
the liquid. If that is the case, we recommend you wet the tubing and run the pump with the
tubing inserted for about 15 to 30 minutes.
8.1 Ordering Spare Parts
8.2 Cassette Material
POM-C Polyoxymethylene-Copolymer:
Good chemical resistance to many organic solvents and strong alkaline chemicals.
Affected by strong acids and oxidizing substances.
UV-stabilized and stable up to temperatures of 80 °C/176 °F (dry, continuous use) or
136 °C/277 °F (dry, for a short time).
PVDF Polyvinylidene fluoride:
Very good chemical resistance to acids and most aliphatic, aromatic, and chlorinated solvents.
Not suitable for long contact with esters, ketones, amines, and strong alkaline chemicals.
Stable both to UV radiation and temperatures up to 110 °C/230 °F (continuous use) or
142 °C/288 °F (for a short time).
8.3 Pressure Lever Cassettes
Note: Due to the configuration of the Reglo ICC Pump, the use of pressure lever cassettes
is not recommended.
9. Repair
The Reglo ICC is designed for many years of trouble free service. The Reglo ICC does not have
any fuses or adjustments inside the pump. If you wish to attempt any repairs after the warranty
period, please contact your local ISMATEC®Distributor for parts.
10. Cleaning
When replacing the tubes and/or cassettes, the pump head should be wiped to avoid any
debris or residue. The pump head can be cleaned with mild detergent water before and after
duty cycles for extended life. The exterior of the pump can be gently wiped with IPA (Isopropyl
Alcohol). Use a 50% mixture of IPA and water on a lint-free soft cloth to clean the LCD screen.
Do not spray or pour any liquid on the LCD screen.
11. Disposal
Please retain packaging materials until the product warranty ends. Afterwards please discard
packaging materials in an environmentally friendly manner according to local regulations. Once
the useful life of the product has ended, please ensure proper disposal according to local laws.
Plastic and electronic components should be disposed of at a recycling facility. Please refer to
local regulations regarding proper disposal.
Order Number Material Cassette
IS3510A POM-C MS/CA Click-n-Go®
IS3610A PVDF MS/CA Click-n-Go®

15 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
12. Technical Specifications
Patent Pending
Drive:
Motor type: Stepper Motor
Flow rate and speed:
Flow rate: 0.0002–35 mL/min
Speed range: 0.1–100 rpm
Digitally adjustable in steps of 0.01 rpm
Differential pressure:
Max. 1.0 bar (14.5 psi)
Remote control:
Via USB 2.0 or RS-232 digital interfaces
Main power connection:
100–240VAC / 50/60 Hz
Power consumption:
Max. 30 W
Operating conditions:
Temperature: +5 to 40 °C (41 to +104 °F)
Rel. humidity max.: 80%
Non-condensing, at normal laboratory conditions
Dimensions/Weight (D x W x H): 205 x 125 x 170 mm (8.1 x 5 x 6.7")
Weight: 2.7 kg (6 lbs)

16 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
Rollers 8
RPM 1.0 100
Tube ID Order No
Flow Rates mL/min.
min. max.
0.13 SC0189T 0.002 0.11
0.19 SC0049T 0.003 0.23
0.25 SC0050T 0.005 0.41
0.38 SC0051T 0.010 0.94
0.44 SC0052T 0.013 1.3
0.51 SC0053T 0.017 1.7
0.57 SC0054T 0.021 2.1
0.64 SC0055T 0.026 2.6
0.76 SC0056T 0.036 3.6
0.89 SC0057T 0.049 4.9
0.95 SC0058T 0.056 5.6
1.02 SC0059T 0.063 6.3
1.09 SC0060T 0.072 7.2
1.14 SC0061T 0.078 7.8
1.22 SC0062T 0.088 8.8
1.3 SC0063T 0.10 10
1.42 SC0064T 0.11 11
1.52 SC0065T 0.13 13
1.65 SC0066T 0.15 15
1.75 SC0067T 0.16 16
1.85 SC0068T 0.17 17
2.06 SC0069T 0.20 20
2.29 SC0070T 0.24 24
2.54 SC0071T 0.27 27
2.79 SC0072T 0.31 31
3.17 SC0224T 0.35 35
Cassettes for the ICC pumps are designed to work with stoppered tubing that fits the MS/CA
Cassettes, commonly referred to as “3-Stop” tubing. Please visit our website or contact your
local distributor for more information on available options.
13. Tubing Size and Flow Rate Chart

17 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
14. Reglo ICC Serial Command Protocol
14.1 Overview
The Reglo Digital model pump supports a 9600 baud, 8 data bits, 1 stop bit, no parity,
no flow control serial communication protocol for controlling the pump’s operation,
as well as setting and getting current pump configuration parameters. This section
describes the protocol used with the Reglo ICC model pump.
The ICC protocol is backwards-compatible with existing controlling devices which use
the Digital protocol. The PC pump-control software uses the protocol described in
this document to control Reglo ICC pumps.
14.2 Physical Layer
The protocol described in this document may be used over USB or RS-232 to communicate with
the Reglo ICC pump. The RS-232 link may be composed of:
Multiple pumps which have been “daisy-chained” together to form a multi-drop RS-232
communications bus. Only one controlling device (i.e. PC) may be connected to this RS-232 bus.
Individual channel control for one pump only
14.3 Message Format
All messages are composed of a string of printable ASCII characters. Each message is
terminated with a carriage return or carriage return and line feed.
14.4 Addressing
The Reglo Digital protocol uses an addressing scheme where each pump is assigned a singular
address. This addressing is designed to accommodate multiple pumps which have been
“daisy-chained” to the same RS-232 bus.
The addressing scheme for the protocol described in the document is dependent on the
physical layer used for communication. This arrangement allows for the same command
definitions to be used for either RS-232 or USB.
When using RS-232 to control the pump in default mode, the addressing scheme of the
Reglo Digital is preserved. The address fields in command messages are used to address
individual pumps connected in a daisy chain. RS-232 can also be used to control the individual
channels as the USB interface does in which case the address character is used to define the
individual channel. Note that when pumps are connected in a daisy chain configuration for
independent channel control, the individual channel control applies to all the pumps.
An example of RS232 commands to address individual channels follows:
@2 Assign address 2 to the pump
2~1 Configures independent channel control, pump 2
1H Channel 1 start (all pumps in a daisy chain)
2S Channel 2 speed setting request returns channel 2 RPM value
2~ query channel status returns 1
2~0 returns pump to standard channel control
2~ query channel status returns 0
2H all channels start

18 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
When using USB to control the pump, the address fields in command messages are used
to address individual pump channels. As each USB connection is a point-to-point link
between the controlling device (PC) and the pump, there is no need to provide pump
addressing in the communications protocol.
Some parameters apply only to each pump, and so individual channel addressing is not
necessary. When using USB, an address must still be provided each of these messages, but
this address will be ignored.
14.5 Definitions
The following symbols are used to represent ASCII characters in the messages defined in this
document.
[CR]—carriage return (0x0d)
[LF]—line feed (0x0a)
[SP]—space (0x20)
[VB]—vertical bar, or “pipe” (0x7c)
14.6 Data Type Formats
The following data formats are used in the subsequent message definitions.
14.6.1 Boolean
Width: 1
Format: A single character indicating True or False.
0 = False
1 = True
14.6.2 Direction
Width: 1
Format: A single character indication direction.
J = Clockwise
K = Counter-clockwise
14.6.3 Discrete Type 1
Width: 1 to 4
Unit: 1
Range: 0 to 9999
Format: Up to four characters representing a discrete integer value in base 10.
Unused digits are not returned.
14.6.4 Discrete Type 2
Width: 4
Units: 1
Range: 0 to 9999
Format: Four characters representing a discrete integer value in base 10. The value is
right-justified. Unused digits to the left are zeros.
14.6.5 Discrete Type 3
Width: 6
Range: 0 to 999999
Format: Six characters in base 10. The value is right-justified. Unused digits to the left
are zeros.

19 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
14.6.6 Discrete Type 4
Width: 10
Range: 0 to 4294967295
Format: Ten characters in base 10. The value is right-justified. Unused digits to the left
are zeros.
14.6.7 Discrete Type 5
Width: 3
Range: 0 to 999
Format: Three characters in base 10. The value is right-justified. Unused digits to the
left are zeros.
14.6.8 Discrete Type 6
Width: 5
Range: 0 to 99999
Format: Five characters in base 10. The value is right-justified. Unused digits to the left
are zeros.
14.6.9 Fractional Type 1
Width: Variable
Format: XXXX.XX—The width of the integer portion of the value is variable. The
decimal point and two digits to the right of the decimal point are always provided.
14.6.10 Volume Type 1
Width: 7
Units: mL
Format: mmmmEse—Represents the scientific notation of m.mmm x 10se. For example,
1.200 x 10-2 is represented with 1200E-2. Note that the decimal point is inferred after
the first character.
mmmm—4 character mantissa
E—“E” character
s—Sign for exponent (+ or -)
e—Single digit exponent
14.6.11 Time Type 1
Width: 1 to 8
Units: 0.1 second
Range: 0 to 35,964,000 (0 to 999 hr)
Format: Up to eight characters representing the time in 0 to 999 hours, with a resolution
of 0.1 sec. Unused digits are not returned.
14.6.12 Time Type 2
Width: 8
Units: 0.1 second
Range: 0 to 35,964,000 (0 to 999 hr)
Format: Eight characters representing the time in 0 to 999 hours, with a resolution of
0.1 s. The value is right-justified. Unused digits to the left are filled with zeros.
14.6.13 String
Width: Variable
Range: 0 to 64 characters
Format: The string is delimited by the delimiting character of the message type that it is
contained in. For device command requests, this is [CR]. For data responses, this is [SP].
The string cannot contain these characters. Other printable ASCII characters are okay.

20 of 36REGLO ICC - ENGLISH , REV. D, 14-036-E
14.6.14 Language
Width: 1
Range: 0–3
Format: A single numeric character representing a language as follows:
0—English
1—French
2—Spanish
3—German
15. Response Messages
Response messages are sent from the pump to the PC in response to request messages.
15.1 Status response
A status response message is the default response of the pump to most commands,
indicating a success or failure of the command.
Response Message: Status
Fields Status
Char Width 1
Status: A single ASCII character indicating the execution status of the requested command.
* (0x2a)—The command was executed successfully.
# (0x23)—The command was not executed successfully.
– (0x2d)—A negative response used for some commands, with different meanings
for each. See individual direct-addressed command definitions for context.
+ (0x2b)—A positive response used for some commands, with different meanings
for each. See individual direct-addressed command definitions for context.
15.2 Data response
A data response is sent when parameter values must be provided in response to a request
message. Each data response is composed of one or more data parameter values which are
delimited by spaces.

Response Message: Data
Fields
Char. Width Variable 1 (optional) Variable (optional) 1 (optional) Variable (optional) 1
Data
Parameter
1
[SP] [SP] [CR] [LF]
Data
Parameter
n
Data
Parameter
2
Data Parameter N—A data value returned by the pump. The format of each data
parameter is defined by the request message that a data response message is sent in
response to. See each request message definition for the definition associated data
response messages.