K-Systems G85 User manual

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K-SYSTEMS
Instruction Manual
CO²Bench Top Incubator G85

Instruction Manual - G85
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Table of contents
1GENERAL INFORMATION & SERVICE ...............................................................................................3
2UNPACKING AND INSPECTION ............................................................................................................4
3ACCESSORIES ............................................................................................................................................4
3.1 STANDARD ACCESSORIES ......................................................................................................................4
3.2 OPTIONAL EQUIPMENT ..........................................................................................................................5
4USER MANUAL...........................................................................................................................................6
4.1 DEFINITION OF USE................................................................................................................................6
4.2 GENERAL DESCRIPTION .........................................................................................................................6
5FEATURES AND OPERATION ................................................................................................................7
5.1 ASSEMBLING THE G85 ..........................................................................................................................7
5.2 CONNECTION TO THE MAINS..................................................................................................................7
5.2.1 Start of apparatus...................................................................................................................7
5.3 KEYBOARD FUNCTIONS .........................................................................................................................8
5.4 OPERATING THE HEATED COMPARTMENT..............................................................................................9
5.5 TEMPERATURE SETTING AND CONTROL.................................................................................................9
5.6 COMBINATION KEYS..............................................................................................................................9
5.7 GAS FLOW SETTING .............................................................................................................................10
5.7.1 Why gassing?........................................................................................................................10
5.7.2 Working gas pressure...........................................................................................................10
5.7.3 Humidifying the gas mixture ................................................................................................11
5.7.4 Purge function ......................................................................................................................13
5.8 TEMPERATURE/FLOW ALARM..............................................................................................................14
5.8.1 Temperature alarm...............................................................................................................14
5.8.2 Gas flow alarm .....................................................................................................................14
5.9 WARMING UP ......................................................................................................................................15
6MENU FUNCTION....................................................................................................................................16
6.1 OVERVIEW OF OPTIONS .......................................................................................................................17
6.2 UNIT ..................................................................................................................................................18
6.3 RS232 .................................................................................................................................................19
6.4 TUNE .................................................................................................................................................20
6.5 INT.T..................................................................................................................................................21
6.6 TI.ST...................................................................................................................................................21
6.7 ST.ST...................................................................................................................................................22
6.8 HEAT .................................................................................................................................................23
6.9 A-ST ...................................................................................................................................................24
6.10 HOUR ............................................................................................................................................25
6.11 REST..............................................................................................................................................26
7USER MAINTENANCE ............................................................................................................................27
7.1 CLEANING ...........................................................................................................................................27
7.2 CALIBRATION......................................................................................................................................27
8TROUBLE SHOOTING ............................................................................................................................28
9TECHNICAL DATA..................................................................................................................................29
10 LIMITED WARRANTY............................................................................................................................30
11 LIABILITY ................................................................................................................................................. 31
12 REPLACEMENT .......................................................................................................................................31

Instruction Manual - G85
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1 General Information & Service
COPYRIGHT
This manual contains information that is subject to copyright. All rights reserved. This
manual should not be photocopied, otherwise copied or distributed, completely or in part,
without the approval of K-SYSTEMS Kivex Biotec.
Users of K-SYSTEMS Kivex Biotec products should not hesitate to contact us if there are
any unclear points or ambiguities in this manual.
K-SYSTEMS
Kivex Biotec A/S
Klintehøj vænge 3-5
3460 Birkerød
Denmark
Tel.: [phone]
Fax: + 45 45 99 56 19
E-mail: [email protected]
Internet: www.K-SYSTEMS.dk
Issue 2
Changenote No: 422
Service address:
Please contact your local K-SYSTEMS Kivex Biotec distributor for details of your nearest
authorized service agent.
CAUTION If the equipment is used in a manner not specified by this manual, the safety of
the user may be at risk and the equipment may be damaged. Always use the
equipment as outlined in this instruction manual.

Instruction Manual - G85
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2 Unpacking and inspection
•Unpack the equipment.
•Check the packing list to insure all accessories are there.
•Examine the packing material carefully for separately packed items.
•Check the incubator for external surface damage.
•Check that the display is not broken or damaged.
•• Check that the lids and gas flow regulator knob operate correctly without
any hindrance.
3 Accessories
3.1 Standard accessories
24 volts main adapter (PSU)
Order code: 51122
Mains Cable
Standard Schuko – Oder code: 52768
UK – Order code: 53886
US – Order code: 52773
Silicone tube
Order code: 11066

Instruction Manual - G85
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3.2 Optional equipment
Optional equipment consists of a range of warming blocks. Please contact your local
distributor or K-SYSTEMS Kivex Biotec for details about the full range of warming blocks.
Warming block B16
Holds two culture dishes
B16F – Falcon 353001
Order code:26059
B16N – NUNC 150318
Order code: 26060
Warming block B24
Holds one center well dish
Falcon 353653
Order code: 26027
Warming block B25
Holds one 60 mm culture dish
Falcon 353002
NUNC 150288
Order code: 26028
Warming block B26
Holds one 4-well Falcon dish with lid
Falcon 353654
Order code: 26031
Warming block B28
Holds one 4-well dish
NUNC 176740
Order code: 26029
Warming block B29
Holds one 5-well dish
MiniTüb 19021/0005
Order code: 26030

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4 User Manual
4.1 Definition of use
The K-SYSTEMS Kivex Biotec G85 CO²Bench Top incubator is designed for keeping
gametes and embryos in a controlled environment.
4.2 General description
The chambers and aluminum warming blocks maintain a preset temperature in the range
ambient to 42°C. The temperature is electronically controlled by a PID controller and can
be read on the displaying increments of 0.1°C.
The G85 features heated lids. The bottom part is made from thick aluminum that gives
ideal temperature distribution. Back part that houses the electronic is made from stainless
steel. Interchangeable aluminum Warming blocks holding a variety of culture dishes are
available.
The G85 features a built in digital flow meter with a possible flow of 0 - 29 liters per hour
and a humidification system.
The G85 has also a purge function enabling a quick CO2level recovery within the
chambers. The recovery time is 15 seconds with a pressure of 0.5 bar.
Note The atmosphere in the chambers may be controlled by flushing with a gas mixture.
Use only 5% CO2in air.

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5 Features and operation
5.1 Assembling the G85
No assembly needed. Just follow directions in section 5.2 and the unit is ready for use.
5.2 Connection to the mains
The mains voltage is automatically regulated to the correct level by the main adapter
(PSU).
Connect the G85 to the mains supply using the enclosed PSU.
For safety reasons the unit has an external PSU which operates at a low voltage.
5.2.1 Start of apparatus
Connect the unit to the PSU and the PSU to the mains wall socket.
To power up the unit, press the heat on button on the keyboard. The display will indicate
the current temperature.
The aluminum dial on the side of the G85 can be turned and the gas flow level is adjusted.
The valve controlling the flow is a precision valve with a short span from closed to open, so
adjust with slow movements. A hint for adjusting is to close it fully and then open it gently.

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5.3 Keyboard functions
Purge
Heat ON/Off
LED Display
Setpoint
Arrow keys UP/DOWN
Alarm

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5.4 Operating the heated compartment
Action Key
Press heat ON/OFF key to
show the actual
temperature.
5.5 Temperature setting and control
Action Key
Press setpoint key and
the set temperature is
shown.
Press and hold setpoint
key.
While holding the
setpoint key in, press
either the arrow UP or
arrow DOWN key to
raise or lower the set
temperature.
When the desired
temperature is
reached, let go of the
setpoint key.
Note The setpoint can be adjusted to a reading between ambient and 49.9 ˚C.
5.6 Combination keys
Action Key Display
Press alarm and UP key. The keyboard is locked.
Press alarm and UP key. The keyboard is
unlocked.
Press Heat ON/OFF and
setpoint key.
Switches between
temperature and time.

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5.7 Gas flow setting
The display can switch between temperature and flow rate.
The flow rate is shown in the display by the integrated digital flow meter in the G85.
Action Key
Press arrow key either up or
down one time.
Now the flow rate in liter per hour
will be shown.
The flow rate can be adjusted between 0 – 29 l/h.
If the flow becomes larger that 29 l/h the display will indicate “Hi” (see section 5.8).
Action Key
Press arrow key either up or
down once again to change to
the display of temperature.
5.7.1 Why gassing?
Most of the culture media which are used in assisted reproduction techniques are sensitive
to changes in pH. Culture media must be maintained at a pH about 7.4. This can be
obtained by gassing with atmosphere of 5% or 6% CO2.
5.7.2 Working gas pressure
Working gas pressure (inlet pressure on the G85) must be regulated to 0.5 bar by a
reduction valve. The reduction valve must be a high quality unit of the pressure type that
can be adjusted precisely in the 0-1 bar range.
The flow rate on the G85 must be adjusted to a specific level in accordance with the use of
the unit.
Frequent openings of the lids will require higher flow rates.
Note Any use of flow type regulators or inadequate quality pressure type regulators will
cause the flow on the G85 to be unstable and drift.
Below is listed the full recovery rates for CO2level in both chambers in minutes.
This can be used as guideline for the use.
Flow (l/h) * 10 28 50
Time (min) ** 12 2.8 0.9
*Flow setting in display of G85.
**Time it will take to recover CO2level after a chamber has been opened at the above flow
setting.