K-Systems G95 User manual

Portable Incubator
G95
K-SYSTEMS
Instruction Manual

Instruction manual - G95
Kivex Biotec Ltd
1. General information and service . . . . . . . . . . . . . . . . . . . . . . . . . .1
2. Unpacking and inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3. Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3.1 Standard accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3.2 Optional equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
4. Users Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.1 Definition of use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.2 General description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.3 Unit performance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
4.4 Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
4.5 General care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
5. Features and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.1 Assembling the G95 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.2 Connection to the mains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.2.1 Start of apparatus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.4 Operating the heated compartment . . . . . . . . . . . . . . . . . . . . .10
5.5 Temperature setting and control. . . . . . . . . . . . . . . . . . . . . . . .10
5.6 Combination keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
5.7 Temperature alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
5.8 Warming up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
5.9 Warming blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6. Menu function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
6.1 Overview of options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
RS232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
TUNE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
INT.T. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Ti.St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
HEAT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
A-St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
HOUR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
REST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
7. Battery functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.1 Charging the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
8. The use of tubes or dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.1 Loading with tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
8.2 Loading with dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
TABLE OF CONTENTS
Continued on next page

Instruction manual - G95
Kivex Biotec Ltd
9. Gassing of CO² incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.1. Why gassing? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.2. Gas supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.3. Gas pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.4. Humidifying the gas mixture . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.5. Gas flow rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.6. Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
10. User maintenace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
10.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
11. Trouble shooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
12. Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
13. Spareparts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
13.1 List of spare part numbers . . . . . . . . . . . . . . . . . . . . . . . . . . .32
13.2 Spare parts - exploded view . . . . . . . . . . . . . . . . . . . . . . . . . .33
14. Limited warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
15. Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
16. Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
TABLE OF CONTENTS

Page 1
Instruction manual - G95
Kivex Biotec Ltd
1. General information and service
COPYRIGHT
This manual contains information that is subject to copyright. All rights reserved. This manual should not
be photocopied, otherwise copied or distributed, completely or in part, without the approval of
K-SYSTEMS
Users of K-SYSTEMS products should not hesitate to contact us if there are any unclear points or ambi-
guities in this manual.
© K-SYSTEMS
Kivex Biotec Ltd
Klintehøj Vænge 3-5
3460 Birkerød
Denmark
Tel.: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.k-systems.dk
Issue 1
Changenote No:275
Service adress:
Please refer to your local K-SYSTEMS distributor for details of your nearest authorised service
agent.
CAUTION If the equipment is used in a manner not specified by this manual, the safety of the
user may be at risk and the equipment may be damaged. Always use the equipment as
outlined in this instruction manual.

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Instruction manual - G95
Kivex Biotec Ltd
2. Unpacking and inspection
Unpack the equipment.•
Check the packing list to insure all accessories are there.•
Examine the packing material carefully for separately packed items.•
Check the incubator for external surface damage.•
Check that the display is not broken or damaged.•
Check that the lid and locking mechanism operate correctly without any hinderence.•
3. Accessories
3.1 Standard accessories
24 volts main adapter (PSU)
Order code: 51115
Mains Cable
Standard Schuko - Order code: 52768
UK - Order code: 53886
US - Order code: 52773
Connection cable for 12 volt car cigarette
lighter socket.
Order code: 51016

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Instruction manual - G95
Kivex Biotec Ltd
Two plastic blocks for holding the warming
blocks in place.
Plastic insulation, white
Order code: 50004
Plastic insulation, black
Order code: 52772
Gas connector.
Order code: 52156, 51086, 52192
Silicone tube.
Order code: 11066

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Instruction manual - G95
Kivex Biotec Ltd
3.2 Optional equipment
Optional equipment consists of a range of warming blocks, flowmeters and gas
humidifiers. K-SYSTEMS recommends that the GH01 is used for supplying gas to the portable incubator.
(see 3.3 Supplementary equipment)
Warming blocks for tubes
Warming block B06
Holds twelve tubes of Ø12 mm
Falcon 352003
Order code: 26006
Warming block B08
Holds ten tubes of Ø17 mm
Falcon 352001
Order code: 26008

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Instruction manual - G95
Kivex Biotec Ltd
Warming blocks for dishes
Warming block B16
Holds two culture dishes
B16F - Falcon 353001
Order code: 26059
B16N - NUNC 150318
Order code: 26060
Warming block B24
Holds one center well dish
Falcon 353653
Order code: 260027
Warming block B25
Holds one 60 mm culture dish
Falcon 353002
NUNC 150288
Order code: 26028
Warming block B26
Holds one 4-well Falcon dish with lid
Falcon 353654
Order code: 26031

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Instruction manual - G95
Kivex Biotec Ltd
Warming block B28
Holds one 4-well dish
NUNC 176740
Order code: 26029
Warming block B29
Holds one 5-well dish
MiniTüb 19021/0005
Order code: 26030
3.3 Supplementary equipment
The G95 is designed as a transport device, and not as a blockwarmer.
It can be used in conjunction with the following K-SYSTEMS products.
GH01
Stand alone humidifier to obtain correct
humidity and warm gas
Order code: 31059
G73
Dry Bath incubator for heating
samples in test tubes
Order code: 22065

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Instruction manual - G95
Kivex Biotec Ltd
4. Users Manual
4.1 Definition of use
The K-SYSTEMS Kivex Biotec G95 Portable incubator is designed for transporting gametes and embryos
which require protection form fluctuations in temperature, pH levels and light.
The warming blocks should be pre warmed to the desired temperature and allowed to stabilise before
placing inside the G95 transport incubator.
The incubator is designed to maintain a stable temperature environment for the transportation of pre
warmed warming blocks.
The temperature indication on the display is that of the maintaining temperature. If cool blocks are
placed within the incubator, sufficient time is required for the temperature to stabilise.
When the unit is not in use it is recommended that it is left on charge.
4.2 General description
The compartment and aluminium warming blocks maintain a preset temperature in the range of
35° to 45° C. The temperature is electronically controlled and can be read on the display in increaments
of 0.1°C resolution. An alarm system provides both visual and audible alarm, if the temperature should
be out of range or in case of low battery (when the mains adapter is not connected).
There are two options of supplying the portable incubator with electric power. Either the incubator can
be connected to the wallsocket using the 24 V external power supply or the incubator can be connected
to a 12 V car cigarette lighter socket.
A built-in battery maintains the temperature for 4,5 to 5,5 hours depending on ambient temperature.
The battery is automatically charged when the incubator is connected to the 24 volt adapter. When the
portable incubator is connected to the car cigarette lighter socket the battery is not charged as the car
generator cannot charge beyond 13,8 volt.
The atmosphere is controlled by flushing with a gas mixture. The gas valve is automatically closed when
the quick connector is disconnected. Interchangeable aluminium warming blocks, holding a variety of
culture dishes and test tubes, are available. The blocks are held in position during transport by a
removable POM block.
RS 232
connector
PSU
socket
Gas inlet
Blanking
plug
Car socket /
GH01

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Instruction manual - G95
Kivex Biotec Ltd
4.3 Unit performance
The unit is connected and allowed to stabilise to the setpoint.
Culture dishes and/or test tubes are placed in the interchangeable warming blocks which are held in
secure positions during transport.
The chamber is purged with the desired gas mixture (regulated for example by the GH01). During
purging, the door is closed, but only sealed after purging. (See page 27)
The gas supply is disconnected from the (quick release) valve at the back of the unit.
4.4 Connections
Should the transport time exceed the charge time (of the built in battery) the unit can be connected to
the cigarette lighter socket found in most automobiles.
Note that the voltage from the car must be 12V, which is standard for cars.
When not in use the unit should be connected to the mains power supply.
The unit can only be heated up using the mains supply.
The car adaptor can only be used to hold the battery power, IT IS NOT A CHARGER.
4.5 General care
Do not obstruct or block the ventilation holes at the back of the unit.
Undue force or pressure should not be put on the front door when it is fully opened.
Ensure that the door hinges are clean and free of any obstructions.
When closing the door, ensure that there are no objects preventing it form closing, as too much force
will damage the closing catches. Thereby hindering its performance.
WARNING This incubator is designed to transport pre warmed blocks and does not have the
capability of warming the blocks in a short period of time.

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Instruction manual - G95
Kivex Biotec Ltd
5. Features and operation
5.1 Assembling the G95
No assembly needed. Just follow directions in 5.2 and the unit is now ready for use.
5.2 Connection to the mains
The mains voltage is automatically regulated to the correct level by the main adapter (PSU).
Connect the G95 to the mains supply using the enclosed PSU.
For safety reasons the unit has an external PSU which operates at a low voltage.
5.2.1 Start of apparatus
Connect the unit to the PSU and the PSU to the mains wall socket.
To power up the unit, press the heat on button on the keyboard. The display will indicate the current
temperature.
5.3 Keyboard functions
Alarm
Heat ON/OFF
LED Display
Setpoint
Arrow keys UP/DOWN

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Instruction manual - G95
Kivex Biotec Ltd
5.4 Operating the heated compartment
Action Key Display
Press heat ON/OFF key to show the actual
temperature.
5.5 Temperature setting and control
The display indicates the average temperature of the chamber.
Action Key
Press setpoint key until set temperature is
shown.
Press and hold setpoint key.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
raise or lower the set temperature.
When the desired temperature is reached,
let go of the setpoint key.
Note The setpoint can be adjusted to a reading between ambient and 49,9° C.

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Instruction manual - G95
Kivex Biotec Ltd
5.6 Combination keys.
Action Key Display
Press alarm and UP key. The keyboard is
locked.
Press alarm and UP key. The keyboard is
unlocked.
Press Heat ON/OFF and setpoint key. Switches between
temperature and
time
Press alarm and setpoint key to go into
powersave mode.
Switches off the
display
Note Powersave mode:
Now only a small LED will light, showing when the heat is active. All other functions
are kept normal. By switching off the display the energy is conserved and the battery
charge holding time prolonged.
5.7 Temperature alarm
The temperature alarm indicates too high or too low temperature.
The alarm will be activated if the temperature rises or falls more than 0.5° C from the setpoint.
There will be an acoustic signal and the red LED light in the alarm key will be activated.
Action Key
Press alarm key to mute the acoustic signal.
Press alarm key once again to bring the
signal back on.
Note The red LED light and the acoustic signal will turn off when the temperature stabilises
to the setpoint level.

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Instruction manual - G95
Kivex Biotec Ltd
5.8 Warming up
Warming up from 20.0 to 39. 0° C takes less than 15 minutes under normal conditions.
When a cold item is placed in the chamber it may need up to 30 minutes to stabilise,
depending on the thermal mass of the object and the initial temperature.
Always let the unit stabilise before use.
5.9 Warming blocks
It is necessary to use warming blocks. Please contact your local distributor or K-SYSTEMS for details
about the full range of warming blocks.
A selection of different tubes and dishes can be transported. Either tubes or dishes can be transported at
one time, not a combination of both.
WARNING Warming blocks should always be pre-heated before usage.
6. Menu function
The user can access a number of advanced functions via the menu.
Action Key Display
Press and hold UP+DOWN key for 3
seconds to enter the the menu.
The first option "UNIT" will appear in the
display.
Press UP/DOWN key to navigate within the
Menu.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
change values within the options.
Press and hold UP+DOWN key for 3
seconds again to exit the menu.

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Instruction manual - G95
Kivex Biotec Ltd
The menu consist of the 10 options listed below.
For further detail see the description on the following pages.
6.1 Overview of options
˚C
˚F
UNIT
On
S-1
S-2
Send
Off
RS232
+0.05
-0.05
TUNE
+0.1
-0.1
INT.T
M + 1
H + 1
Ti.St
M + 1
H + 1
St.St
On
Off
HEAT
On
Off
A-St
On
Off
HOUR
REST ----
Software version
Calib. value
}Version 3

Page 14
Instruction manual - G95
Kivex Biotec Ltd
UNIT
In the UNIT option the displayed temperature unit can be selected to be either
degrees Celsius or Fahrenheit.
Follow these steps to navigate in the UNIT option.
Action Key Display
Press and hold UP+DOWN key for 3
seconds to enter the the menu.
The option "UNIT" will appear in the
display.
Press and hold setpoint key to change
values.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
select the required type of unit.
When the required type has been selected
let go of the setpoint key.
Press and hold UP+DOWN key for 3
seconds to exit the the menu.

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Instruction manual - G95
Kivex Biotec Ltd
RS232
The RS232 option is for serial communication and data logging of the temperature
and alarm conditions.
Follow these steps to navigate in the RS232 option.
Action Key Display
Press and hold UP+DOWN key for 3
seconds to enter the the menu.
The option "UNIT" will appear in the
display.
Press DOWN key until the option "RS232"
appears in the display.
Press and hold setpoint key to change
values.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
select between "ON" and "OFF".
"OFF" : The RS232 function is turned off.
"ON": Data is send once every two seconds.
When the required setting is activated, let
go of the setpoint key.
Press and hold UP+DOWN key for 3
seconds to exit the the menu.
Data logging the temperature valves during use:
Note The RS232 cable must be connected before start.
Follow the steps in the table above and choose "ON".
Choose one of the settings to get the required data
S-1 Sends the data for the last one hour.
S-2 Sends the data for the last two hours
send Sends the data for the last five hours.
Note The use of this function warrants extra equipment software (Ver. 3) and on some base
models addition of a Serial PCB. The parameters on or off will not affect normal usage
when serial communication is not connected.

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Instruction manual - G95
Kivex Biotec Ltd
TUNE
The TUNE option is for calibration of the displayed temperature.
If there is an offset between the value on the display and any measurements made with a high precision
external temperature sensor, this can be corrected.
The new temperature reading will be kept as the displayed value, and temperature controlling conduct-
ed on this basis. When the power (incl. battery) is disconnected default values will be restored.
Follow these steps to navigate in the TUNE option.
Action Key Display
Press and hold UP+DOWN key for 3
seconds to enter the the menu.
The option "UNIT" will appear in the
display.
Press DOWN key until the option "TUNE"
appears in the display.
Press and hold setpoint key to change
values.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
select the value of temperature.
When the required temperature has been
selected let go of the setpoint key.
Press and hold UP+DOWN key for 3
seconds to exit the the menu.
To see the calibrate value
Press and hold setpoint key.
While holding the setpoint key in, press the
HEAT key
The calibrate value
is shown
CAUTION Any change in the factory set calibration should always be based on very certain
temperature measurements. K-SYSTEMS only recommend the use of a high quality
PT-100 sensor that is calibrated with the precision instrument used for measuring
according to the manufacturers specifications. Also ensure an optimal contact between
the sensor and the place measured. Use only a sensor type that is correct for the
purpose and of correct size. If in doubt contact your local distributor or K-SYSTEMS.

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Instruction manual - G95
Kivex Biotec Ltd
INT.T
The INT.T (Integral Time) option is for changing the base value for the PID controller.
This should not be attempted by unauthorised persons. If set at a different level the controlling principle
will be affected.
From the factory it is set to a closely calculated value specific for the model.
In version 3, this value is locked and can't be changed.
CAUTION Do not change the value here. If by mistake any changes are made or doubts occur if the
value are correct please see the REST function in the menu for the restore of the defaults
Ti.St
The Ti.St (Time Set) option is for setting the time.
Follow these steps to navigate in the Ti.St option.
Action Key Display
Press and hold UP+DOWN key for 3
seconds to enter the the menu.
The option "UNIT" will appear in the
display.
Press DOWN key until the option "Ti.St"
appears in the display.
Press and hold setpoint key to change
values.
While holding the setpoint key in, press
either the arrow UP or arrow DOWN key to
change the hour.
When the time is set, let go of the setpoint
key.
Press and hold UP+DOWN key for 3
seconds to exit the the menu.