K-Systems G95 User manual

Portable Incubator
G95
K-SYSTEMS
Instruction Manual

Instruction manual - G95
Kivex Biotec Ltd
1. General information and service . . . . . . . . . . . . . . . . . . . . . . . . . .1
2. Unpacking and inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3. Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3.1 Standard accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
3.2 Optional equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
4. Users Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.1 Definition of use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.2 General description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
4.3 Unit performance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
4.4 Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
4.5 General care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
5. Features and operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.1 Assembling the G95 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.2 Connection to the mains . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.2.1 Start of apparatus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
5.4 Operating the heated compartment . . . . . . . . . . . . . . . . . . . . .10
5.5 Temperature setting and control. . . . . . . . . . . . . . . . . . . . . . . .10
5.6 Combination keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
5.7 Temperature alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
5.8 Warming up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
5.9 Warming blocks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6. Menu function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
6.1 Overview of options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
UNIT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
RS232 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
TUNE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
INT.T. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Ti.St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
HEAT. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
A-St . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
HOUR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
REST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
7. Battery functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.1 Charging the battery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
8. The use of tubes or dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.1 Loading with tubes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
8.2 Loading with dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
TABLE OF CONTENTS
Continued on next page

Instruction manual - G95
Kivex Biotec Ltd
9. Gassing of CO² incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.1. Why gassing? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.2. Gas supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.3. Gas pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.4. Humidifying the gas mixture . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.5. Gas flow rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
9.6. Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
10. User maintenace . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
10.2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
11. Trouble shooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
12. Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
13. Spareparts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
13.1 List of spare part numbers . . . . . . . . . . . . . . . . . . . . . . . . . . .32
13.2 Spare parts - exploded view . . . . . . . . . . . . . . . . . . . . . . . . . .33
14. Limited warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
15. Liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
16. Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
TABLE OF CONTENTS

Page 1
Instruction manual - G95
Kivex Biotec Ltd
1. General information and service
COPYRIGHT
This manual contains information that is subject to copyright. All rights reserved. This manual should not
be photocopied, otherwise copied or distributed, completely or in part, without the approval of
K-SYSTEMS
Users of K-SYSTEMS products should not hesitate to contact us if there are any unclear points or ambi-
guities in this manual.
© K-SYSTEMS
Kivex Biotec Ltd
Klintehøj Vænge 3-5
3460 Birkerød
Denmark
Tel.: [phone]
Fax: [phone]
E-mail: [e-mail]
Internet: www.k-systems.dk
Issue 1
Changenote No:275
Service adress:
Please refer to your local K-SYSTEMS distributor for details of your nearest authorised service
agent.
CAUTION If the equipment is used in a manner not specified by this manual, the safety of the
user may be at risk and the equipment may be damaged. Always use the equipment as
outlined in this instruction manual.

Page 2
Instruction manual - G95
Kivex Biotec Ltd
2. Unpacking and inspection
Unpack the equipment.•
Check the packing list to insure all accessories are there.•
Examine the packing material carefully for separately packed items.•
Check the incubator for external surface damage.•
Check that the display is not broken or damaged.•
Check that the lid and locking mechanism operate correctly without any hinderence.•
3. Accessories
3.1 Standard accessories
24 volts main adapter (PSU)
Order code: 51115
Mains Cable
Standard Schuko - Order code: 52768
UK - Order code: 53886
US - Order code: 52773
Connection cable for 12 volt car cigarette
lighter socket.
Order code: 51016

Page 3
Instruction manual - G95
Kivex Biotec Ltd
Two plastic blocks for holding the warming
blocks in place.
Plastic insulation, white
Order code: 50004
Plastic insulation, black
Order code: 52772
Gas connector.
Order code: 52156, 51086, 52192
Silicone tube.
Order code: 11066

Page 4
Instruction manual - G95
Kivex Biotec Ltd
3.2 Optional equipment
Optional equipment consists of a range of warming blocks, flowmeters and gas
humidifiers. K-SYSTEMS recommends that the GH01 is used for supplying gas to the portable incubator.
(see 3.3 Supplementary equipment)
Warming blocks for tubes
Warming block B06
Holds twelve tubes of Ø12 mm
Falcon 352003
Order code: 26006
Warming block B08
Holds ten tubes of Ø17 mm
Falcon 352001
Order code: 26008

Page 5
Instruction manual - G95
Kivex Biotec Ltd
Warming blocks for dishes
Warming block B16
Holds two culture dishes
B16F - Falcon 353001
Order code: 26059
B16N - NUNC 150318
Order code: 26060
Warming block B24
Holds one center well dish
Falcon 353653
Order code: 260027
Warming block B25
Holds one 60 mm culture dish
Falcon 353002
NUNC 150288
Order code: 26028
Warming block B26
Holds one 4-well Falcon dish with lid
Falcon 353654
Order code: 26031

Page 6
Instruction manual - G95
Kivex Biotec Ltd
Warming block B28
Holds one 4-well dish
NUNC 176740
Order code: 26029
Warming block B29
Holds one 5-well dish
MiniTüb 19021/0005
Order code: 26030
3.3 Supplementary equipment
The G95 is designed as a transport device, and not as a blockwarmer.
It can be used in conjunction with the following K-SYSTEMS products.
GH01
Stand alone humidifier to obtain correct
humidity and warm gas
Order code: 31059
G73
Dry Bath incubator for heating
samples in test tubes
Order code: 22065

Page 7
Instruction manual - G95
Kivex Biotec Ltd
4. Users Manual
4.1 Definition of use
The K-SYSTEMS Kivex Biotec G95 Portable incubator is designed for transporting gametes and embryos
which require protection form fluctuations in temperature, pH levels and light.
The warming blocks should be pre warmed to the desired temperature and allowed to stabilise before
placing inside the G95 transport incubator.
The incubator is designed to maintain a stable temperature environment for the transportation of pre
warmed warming blocks.
The temperature indication on the display is that of the maintaining temperature. If cool blocks are
placed within the incubator, sufficient time is required for the temperature to stabilise.
When the unit is not in use it is recommended that it is left on charge.
4.2 General description
The compartment and aluminium warming blocks maintain a preset temperature in the range of
35° to 45° C. The temperature is electronically controlled and can be read on the display in increaments
of 0.1°C resolution. An alarm system provides both visual and audible alarm, if the temperature should
be out of range or in case of low battery (when the mains adapter is not connected).
There are two options of supplying the portable incubator with electric power. Either the incubator can
be connected to the wallsocket using the 24 V external power supply or the incubator can be connected
to a 12 V car cigarette lighter socket.
A built-in battery maintains the temperature for 4,5 to 5,5 hours depending on ambient temperature.
The battery is automatically charged when the incubator is connected to the 24 volt adapter. When the
portable incubator is connected to the car cigarette lighter socket the battery is not charged as the car
generator cannot charge beyond 13,8 volt.
The atmosphere is controlled by flushing with a gas mixture. The gas valve is automatically closed when
the quick connector is disconnected. Interchangeable aluminium warming blocks, holding a variety of
culture dishes and test tubes, are available. The blocks are held in position during transport by a
removable POM block.
RS 232
connector
PSU
socket
Gas inlet
Blanking
plug
Car socket /
GH01