Kikkoman LUMITESTER C-110 User manual

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OPERATION MANUAL
Thank you for purchasing the LUMITESTER C-110.
To use your LUMITESTER C-110 safely and correctly,
read this operation manual carefully and follows the instructions.
Kikkoman Corporation
Ⓒ2009 Kikkoman Corp. (04-1)

To ensure safe use
Safety precautions
WARNING "WARNING" indicates a potentially hazardous situation which, if not
avoided, could result in death or serious injury.
CAUTION "CAUTION" indicates a potentially hazardous situation which, if not
avoided, could result in minor or moderate injury or damage to the
equipment.
In addition to the above alert symbols and signal words, the following warning symbols are used
in this manual to designate the degree of hazard and damage that might occur if this product is
used incorrectly.
Mandatory
This symbol indicates a mandatory action.
Prohibited
This symbol indicates a prohibited action.
Electrical shock hazard
This symbol indicates a risk of electrical shock.
Fire hazard
This symbol indicates a risk of smoke emission or fire.
Explosive hazard
This symbol indicates a risk of explosion.
Toxic hazard
This symbol indicates a toxic substance risk.
Corrosion hazard
This symbol indicates a risk of corrosion.
Ⓒ2009 Kikkoman Corp. (04-1)

Handling precautions
If an abnormal condition is found…
WARNING
If an abnormal condition is found, immediately turn off the power.
If operating the unit on battery power at that time, then remove the battery. If
operating it from an AC adapter, then turn off the power and unplug the AC adapter
from the power socket.
If you notice abnormal operation, or burning odor or smoke being emitted from this
unit, then there is a risk of potential fire or internal explosion. After checking that the
smoke has dissipated, please contact us or our local dealer. Never attempt to repair
the problem yourself since this could be dangerous. Continuous operation under
abnormal conditions may lead to fire or electrical shock.
Precautions during use
WARNING
Do not use chemicals that may generate flammable gases and do not use in an
atmosphere containing flammable gases.
Doing so may cause gas explosion in this unit.
Never attempt to remove any cover of this unit, or disassemble or repair any part of
this product. Only our qualified service personnel are allowed to service this unit.
This unit contains high internal voltages, so improper handling may cause electrical
shock, fire or abnormal operation.
Do not plug or unplug the AC adapter with wet or moist hands.
Doing so may cause electrical shock, fire or malfunction.
When using the AC adapter, always connect it to a power source ranging from AC
100V to 240V (50/60Hz).
Failure to follow this instruction may cause electrical shock or fire, or damage this
unit.
Use the specified AC adapter or battery that comes with this unit.
Using a different AC adapter or battery may cause electrical shock or fire, or
damage this unit.
Never connect an AC power source to the AC adapter input and RS-232C terminal.
Doing so may cause electrical shock, fire or malfunction.
Do not expose this unit to water and do not handle it with wet hands.
This unit is not water-proof, so water or moisture may cause electrical shock, fire or
malfunction.
Ⓒ2009 Kikkoman Corp. (04-1)

Other precautions during use
Take the following precautions when using this unit.
• Do not move this unit during measurement. This may cause fluctuations in the measurement
data.
• Do not tilt this unit during measurement. This may cause fluctuations in the measurement
data.
• Use this unit while placed on a level surface.
• After measurement, always take the measurement container out of this unit. If this unit is
moved or inverted after measurement while the measurement container is left inside, then the
remaining reagent may contaminate the measurement section, possibly causing malfunction.
• Keep this unit at least 10 cm away from stirrers, mixers or any device that may generate
electromagnetic noise.
• Do not expose this unit to liquids, reagents or organic solvents. Doing so may cause
malfunction, discoloration or deformation. If the unit was exposed to such liquids, reagents or
solvents, then wipe them away immediately.
• Do not press or rub the display panel or key operation section with hard or pointed objects.
Doing so may cause scratches or damages.
• Before connecting to the RS-232C terminal on this unit, make sure the power is off.
• Before using this unit, wash your hands thoroughly or wear sterilized gloves. This unit is
extremely sensitive, so the ATP (Adenosine Tri-Phosphate) may become contaminated
through contamination from hands.
• Avoid conversation with others when you are using this unit. This unit is extremely sensitive,
so the ATP may become contaminated through mouth saliva.
• Always use the specified instruments and tools.
• Use this unit at temperatures from 5 to 40ºC and a humidity from 20 to 85%.
• Do not use this unit in locations where the temperature fluctuates rapidly.
• Do not use this unit in locations directly exposed to steam or in locations where condensation
occurs.
• Do not use this unit in locations where corrosive gases are generated.
• Do not use this unit in locations subject to vibration.
• Do not use this unit in locations exposed to excessive dust or debris.
• Do not leave this unit in unstable place or hazardous locations. Do not apply excessive shock
to this unit and do not drop it.
• Do not place this unit in locations where the temperature is extremely low or near heaters.
• Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan.
• Do not place any object on this unit.
• Be careful to prevent electrostatic charge buildup on the sample tube. Static electricity may
cause an increase the measurement data.
Ⓒ2009 Kikkoman Corp. (04-1)

Precautions when using reagents
CAUTION
Wear gloves and protective glasses and mask as needed, and provide sufficient
ventilation when handling the reagent.
Flying droplets from reagents or samples may cause skin or eye irritation or injury. If
an irritant reagent comes in contact with the skin or eyes, wash thoroughly with tap
water and consult a physician or pharmacist.
Precaution during installation or storage
WARNING
Do not use chemicals that may generate flammable gases and do not store this unit
in an atmosphere containing flammable gases.
Doing so may cause this unit to explode internally.
Do not store this unit in locations where water or chemicals may penetrate inside
this unit.
A short-circuit may occur if water or chemicals penetrate inside this unit, causing fire
or electrical shock.
Ⓒ2009 Kikkoman Corp. (04-1)

Other precautions during storage
When storing this unit, take the following precautions:
• Store this unit at temperatures from -10 to +50ºC and a humidity from 20 to 95%.
• Do not store this unit in locations directly exposed to steam and in locations where
condensation occurs.
• Do not store this unit in locations where corrosive gases are generated.
• Do not use this unit in locations subject to vibration.
• Do not use this unit in locations exposed to excessive dust or debris.
• Do not leave this unit in unstable or hazardous locations. Do not apply excessive shock to
this unit and do not drop it.
• Do not place this unit in locations where the temperature is extremely low or near heaters.
• Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan.
• Do not place any object on this unit.
When moving or transporting this unit
When moving or transporting this unit, take the following precautions:
Always use the original carton and packing materials when transporting this unit. Our warranty
does not cover any damage or breakdown caused during transportation due to use of
non-specified cartons and packing materials.
When moving this unit, always first turn off the power and unplug the AC adapter from the
power socket.
Disposal of this unit and reagents
When disposing of this unit and reagents be sure to comply with local regulations regarding
industrial waste disposal. Consult the local regulations in your area for detailed information.
WARNING
Do not place this unit in fire or attempt to burn it.
Doing so may cause the unit to explode internally.
Ⓒ2009 Kikkoman Corp. (04-1)

Ⓒ2009 Kikkoman Corp. (04-1)

Table of Contents
1. Overview .............................................................................................................1
2. Features ..............................................................................................................1
3. Part Names and Functions ..................................................................................2
4. Power ..................................................................................................................4
5. Operating Procedure ...........................................................................................5
5-1 Basic operation.............................................................................................5
5-1-1 STANDARD mode measurement .....................................................5
5-1-2 ADVANCED mode measurement .....................................................7
5-2 Function routines..........................................................................................9
(1) Print [PRINT OUT] ................................................................................10
(2) Data clear [CLEAR ALL DATA]..............................................................12
(3) RS-232C transmission [TRANS ALL DATA] ..........................................13
(4) Various settings [SET-UP] .....................................................................14
(5) Display unit selection [CHOOSE A UNIT]..............................................16
(6) ATP/CELL coefficient selection [CHOOSE ATP/CELL]..........................17
(7) ATP/CELL coefficient input [INPUT ATP/CELL].....................................18
(8) "pg" conversion coefficient input [INPUT COEF.(pg)] ............................19
(9) Calibration curve input [INPUT CAL.CURVE] .......................................20
(10) Measurement time setting [MEASURING TIME].................................21
(11) Monitor mode [MONITOR MODE].......................................................22
6. Relation between Each Unit ..............................................................................23
6-1 ATP concentrations ....................................................................................23
6-2 ATP amount (pg) ........................................................................................24
6-3 Number of cells (CELL) ..............................................................................24
7. Computer Interface............................................................................................25
8. Maintenance......................................................................................................26
8-1 Cleaning the measurement chamber .........................................................26
8-2 Replacing the rechargeable battery pack ...................................................26
9. Specifications ....................................................................................................27
10. Dimensions........................................................................................................28
11. After Sales Service ............................................................................................29
Ⓒ2009 Kikkoman Corp. (04-1)

1. Overview
The LUMITESTER C-110 is a portable luminometer designed for simple and quick
measurement of light emission by ATP. Using a compact photomultiplier tube as the light
detector, the LUMITESTER C-110 measures low-level light by photon counting method that
ensures high sensitivity and low noise.
The LUMITESTER C-110 operates with either a rechargeable battery or AC adapter, allowing
wide flexibility with regard to measurement locations.
Operation is very simple. All you need to begin measurement is just insert a sample tube and
press the start key.
Measurement units are selectable from among RLU (relative light units), M, pg and CELL.
The dedicated utility software allows easy creation and registration of a calibration curve, data
transfer, etc.
2. Features
• High sensitivity
Using a special reagent enables ATP measurement at 4×10-12M (0.2 pg / assay). (Note 1)
• Wide measurement range
ATP amounts can be measured over a wide range of 4×10-12 to 1×10-6M. (Note 1)
• Quick and simple measurement
Just pressing one key starts measurement and the result is displayed in 10 seconds (when
in STANDARD mode).
• Data memory
Up to 1000 data can be stored.
• ATP amount display
ATP amounts (M or pg) can be displayed by pre-registering a calibration curve. The
number of cells can also be displayed by registering the APT amount per cell.
• Calibration curve input
The dedicated utility software allows creating and registering a calibration curve.
• ADVANCED mode
ADVANCED mode sets the measurement time and displays count values in real-time.
Note 1: Sensitivity and measurement range might differ depending on the reagent being
used.
Ⓒ Kikkoman Corp. (04—1)
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3. Part Names and Functions
(3) Measurement
chambe
r
(5) Operation panel
(1) Main body
(4) Display panel
(7) Connector for AC adapter
(6) RS-232C connector
(8) Battery charge LED
(2) Cove
r
(1) Main body The LUMITESTER main body containing the detector and
drive circuits.
(2) Cover The cover on the measurement chamber. It can be
opened and closed with the touch of a finger.
(3) Measurement chamber A sample tube is installed in this chamber.
(4) Display panel Displays measurement results and instructions.
(5) Operation panel See description on the next page.
(6) RS-232C connector Connects to a printer or computer.
(7) Connector for AC adapter Connects to the AC adapter.
(8) Battery charge LED This LED lamp is lit in red during charging of the battery
and turns green when charging is complete.
Accessories
AC adapter Use this AC adapter when operating the LUMITESTER
from the AC line.
RS-232C cable Use this cable to connect to a printer or computer.
CD Contains utility software.
Ⓒ Kikkoman Corp. (04—1)
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