Kikkoman LUMITESTER C-110 User manual

L
LU UM MI IT TE ES ST TE ER R
C
C- -1 11 10 0
OPERATION MANUAL
Thank you for purchasing the LUMITESTER C-110.
To use your LUMITESTER C-110 safely and correctly,
read this operation manual carefully and follows the instructions.
Kikkoman Corporation
Ⓒ2009 Kikkoman Corp. (04-1)

To ensure safe use
Safety precautions
WARNING "WARNING" indicates a potentially hazardous situation which, if not
avoided, could result in death or serious injury.
CAUTION "CAUTION" indicates a potentially hazardous situation which, if not
avoided, could result in minor or moderate injury or damage to the
equipment.
In addition to the above alert symbols and signal words, the following warning symbols are used
in this manual to designate the degree of hazard and damage that might occur if this product is
used incorrectly.
Mandatory
This symbol indicates a mandatory action.
Prohibited
This symbol indicates a prohibited action.
Electrical shock hazard
This symbol indicates a risk of electrical shock.
Fire hazard
This symbol indicates a risk of smoke emission or fire.
Explosive hazard
This symbol indicates a risk of explosion.
Toxic hazard
This symbol indicates a toxic substance risk.
Corrosion hazard
This symbol indicates a risk of corrosion.
Ⓒ2009 Kikkoman Corp. (04-1)

Handling precautions
If an abnormal condition is found…
WARNING
If an abnormal condition is found, immediately turn off the power.
If operating the unit on battery power at that time, then remove the battery. If
operating it from an AC adapter, then turn off the power and unplug the AC adapter
from the power socket.
If you notice abnormal operation, or burning odor or smoke being emitted from this
unit, then there is a risk of potential fire or internal explosion. After checking that the
smoke has dissipated, please contact us or our local dealer. Never attempt to repair
the problem yourself since this could be dangerous. Continuous operation under
abnormal conditions may lead to fire or electrical shock.
Precautions during use
WARNING
Do not use chemicals that may generate flammable gases and do not use in an
atmosphere containing flammable gases.
Doing so may cause gas explosion in this unit.
Never attempt to remove any cover of this unit, or disassemble or repair any part of
this product. Only our qualified service personnel are allowed to service this unit.
This unit contains high internal voltages, so improper handling may cause electrical
shock, fire or abnormal operation.
Do not plug or unplug the AC adapter with wet or moist hands.
Doing so may cause electrical shock, fire or malfunction.
When using the AC adapter, always connect it to a power source ranging from AC
100V to 240V (50/60Hz).
Failure to follow this instruction may cause electrical shock or fire, or damage this
unit.
Use the specified AC adapter or battery that comes with this unit.
Using a different AC adapter or battery may cause electrical shock or fire, or
damage this unit.
Never connect an AC power source to the AC adapter input and RS-232C terminal.
Doing so may cause electrical shock, fire or malfunction.
Do not expose this unit to water and do not handle it with wet hands.
This unit is not water-proof, so water or moisture may cause electrical shock, fire or
malfunction.
Ⓒ2009 Kikkoman Corp. (04-1)

Other precautions during use
Take the following precautions when using this unit.
• Do not move this unit during measurement. This may cause fluctuations in the measurement
data.
• Do not tilt this unit during measurement. This may cause fluctuations in the measurement
data.
• Use this unit while placed on a level surface.
• After measurement, always take the measurement container out of this unit. If this unit is
moved or inverted after measurement while the measurement container is left inside, then the
remaining reagent may contaminate the measurement section, possibly causing malfunction.
• Keep this unit at least 10 cm away from stirrers, mixers or any device that may generate
electromagnetic noise.
• Do not expose this unit to liquids, reagents or organic solvents. Doing so may cause
malfunction, discoloration or deformation. If the unit was exposed to such liquids, reagents or
solvents, then wipe them away immediately.
• Do not press or rub the display panel or key operation section with hard or pointed objects.
Doing so may cause scratches or damages.
• Before connecting to the RS-232C terminal on this unit, make sure the power is off.
• Before using this unit, wash your hands thoroughly or wear sterilized gloves. This unit is
extremely sensitive, so the ATP (Adenosine Tri-Phosphate) may become contaminated
through contamination from hands.
• Avoid conversation with others when you are using this unit. This unit is extremely sensitive,
so the ATP may become contaminated through mouth saliva.
• Always use the specified instruments and tools.
• Use this unit at temperatures from 5 to 40ºC and a humidity from 20 to 85%.
• Do not use this unit in locations where the temperature fluctuates rapidly.
• Do not use this unit in locations directly exposed to steam or in locations where condensation
occurs.
• Do not use this unit in locations where corrosive gases are generated.
• Do not use this unit in locations subject to vibration.
• Do not use this unit in locations exposed to excessive dust or debris.
• Do not leave this unit in unstable place or hazardous locations. Do not apply excessive shock
to this unit and do not drop it.
• Do not place this unit in locations where the temperature is extremely low or near heaters.
• Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan.
• Do not place any object on this unit.
• Be careful to prevent electrostatic charge buildup on the sample tube. Static electricity may
cause an increase the measurement data.
Ⓒ2009 Kikkoman Corp. (04-1)

Precautions when using reagents
CAUTION
Wear gloves and protective glasses and mask as needed, and provide sufficient
ventilation when handling the reagent.
Flying droplets from reagents or samples may cause skin or eye irritation or injury. If
an irritant reagent comes in contact with the skin or eyes, wash thoroughly with tap
water and consult a physician or pharmacist.
Precaution during installation or storage
WARNING
Do not use chemicals that may generate flammable gases and do not store this unit
in an atmosphere containing flammable gases.
Doing so may cause this unit to explode internally.
Do not store this unit in locations where water or chemicals may penetrate inside
this unit.
A short-circuit may occur if water or chemicals penetrate inside this unit, causing fire
or electrical shock.
Ⓒ2009 Kikkoman Corp. (04-1)

Other precautions during storage
When storing this unit, take the following precautions:
• Store this unit at temperatures from -10 to +50ºC and a humidity from 20 to 95%.
• Do not store this unit in locations directly exposed to steam and in locations where
condensation occurs.
• Do not store this unit in locations where corrosive gases are generated.
• Do not use this unit in locations subject to vibration.
• Do not use this unit in locations exposed to excessive dust or debris.
• Do not leave this unit in unstable or hazardous locations. Do not apply excessive shock to
this unit and do not drop it.
• Do not place this unit in locations where the temperature is extremely low or near heaters.
• Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan.
• Do not place any object on this unit.
When moving or transporting this unit
When moving or transporting this unit, take the following precautions:
Always use the original carton and packing materials when transporting this unit. Our warranty
does not cover any damage or breakdown caused during transportation due to use of
non-specified cartons and packing materials.
When moving this unit, always first turn off the power and unplug the AC adapter from the
power socket.
Disposal of this unit and reagents
When disposing of this unit and reagents be sure to comply with local regulations regarding
industrial waste disposal. Consult the local regulations in your area for detailed information.
WARNING
Do not place this unit in fire or attempt to burn it.
Doing so may cause the unit to explode internally.
Ⓒ2009 Kikkoman Corp. (04-1)

Ⓒ2009 Kikkoman Corp. (04-1)

Table of Contents
1. Overview .............................................................................................................1
2. Features ..............................................................................................................1
3. Part Names and Functions ..................................................................................2
4. Power ..................................................................................................................4
5. Operating Procedure ...........................................................................................5
5-1 Basic operation.............................................................................................5
5-1-1 STANDARD mode measurement .....................................................5
5-1-2 ADVANCED mode measurement .....................................................7
5-2 Function routines..........................................................................................9
(1) Print [PRINT OUT] ................................................................................10
(2) Data clear [CLEAR ALL DATA]..............................................................12
(3) RS-232C transmission [TRANS ALL DATA] ..........................................13
(4) Various settings [SET-UP] .....................................................................14
(5) Display unit selection [CHOOSE A UNIT]..............................................16
(6) ATP/CELL coefficient selection [CHOOSE ATP/CELL]..........................17
(7) ATP/CELL coefficient input [INPUT ATP/CELL].....................................18
(8) "pg" conversion coefficient input [INPUT COEF.(pg)] ............................19
(9) Calibration curve input [INPUT CAL.CURVE] .......................................20
(10) Measurement time setting [MEASURING TIME].................................21
(11) Monitor mode [MONITOR MODE].......................................................22
6. Relation between Each Unit ..............................................................................23
6-1 ATP concentrations ....................................................................................23
6-2 ATP amount (pg) ........................................................................................24
6-3 Number of cells (CELL) ..............................................................................24
7. Computer Interface............................................................................................25
8. Maintenance......................................................................................................26
8-1 Cleaning the measurement chamber .........................................................26
8-2 Replacing the rechargeable battery pack ...................................................26
9. Specifications ....................................................................................................27
10. Dimensions........................................................................................................28
11. After Sales Service ............................................................................................29
Ⓒ2009 Kikkoman Corp. (04-1)

1. Overview
The LUMITESTER C-110 is a portable luminometer designed for simple and quick
measurement of light emission by ATP. Using a compact photomultiplier tube as the light
detector, the LUMITESTER C-110 measures low-level light by photon counting method that
ensures high sensitivity and low noise.
The LUMITESTER C-110 operates with either a rechargeable battery or AC adapter, allowing
wide flexibility with regard to measurement locations.
Operation is very simple. All you need to begin measurement is just insert a sample tube and
press the start key.
Measurement units are selectable from among RLU (relative light units), M, pg and CELL.
The dedicated utility software allows easy creation and registration of a calibration curve, data
transfer, etc.
2. Features
• High sensitivity
Using a special reagent enables ATP measurement at 4×10-12M (0.2 pg / assay). (Note 1)
• Wide measurement range
ATP amounts can be measured over a wide range of 4×10-12 to 1×10-6M. (Note 1)
• Quick and simple measurement
Just pressing one key starts measurement and the result is displayed in 10 seconds (when
in STANDARD mode).
• Data memory
Up to 1000 data can be stored.
• ATP amount display
ATP amounts (M or pg) can be displayed by pre-registering a calibration curve. The
number of cells can also be displayed by registering the APT amount per cell.
• Calibration curve input
The dedicated utility software allows creating and registering a calibration curve.
• ADVANCED mode
ADVANCED mode sets the measurement time and displays count values in real-time.
Note 1: Sensitivity and measurement range might differ depending on the reagent being
used.
Ⓒ Kikkoman Corp. (04—1)
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3. Part Names and Functions
(3) Measurement
chambe
r
(5) Operation panel
(1) Main body
(4) Display panel
(7) Connector for AC adapter
(6) RS-232C connector
(8) Battery charge LED
(2) Cove
r
(1) Main body The LUMITESTER main body containing the detector and
drive circuits.
(2) Cover The cover on the measurement chamber. It can be
opened and closed with the touch of a finger.
(3) Measurement chamber A sample tube is installed in this chamber.
(4) Display panel Displays measurement results and instructions.
(5) Operation panel See description on the next page.
(6) RS-232C connector Connects to a printer or computer.
(7) Connector for AC adapter Connects to the AC adapter.
(8) Battery charge LED This LED lamp is lit in red during charging of the battery
and turns green when charging is complete.
Accessories
AC adapter Use this AC adapter when operating the LUMITESTER
from the AC line.
RS-232C cable Use this cable to connect to a printer or computer.
CD Contains utility software.
Ⓒ Kikkoman Corp. (04—1)
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Operation Panel
ON/OFF
(2) <F> key
ENTER
F
(3) <Scroll> key (4) <ENTER> key
(1) <POWER> key
(1) <POWER> key Press this key to turn power on or off.
(2) <F> key Press this key to enter the function routine mode.
(3) <Scroll> key Use this key to scroll the measurement data or select test conditions.
(4) <ENTER> key Press this key to begin measurement or enable data entry.
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4. Power
<LUMITESTER operation>
The LUMITESTER can operate either from the rechargeable battery or AC adapter.
<When the AC adapter is not connected>
The LUMITESTER operates from the battery.
<When the AC adapter is connected>
The LUMITESTER operates from the AC adapter.
<Battery charge condition>
The battery is charged by the AC adapter, and the LED indicator changes during charging
as follows:
Red during charging →Green when fully charged →Charging stops
<Charging time> The charging time is about 4 hours.
<To connect the AC adapter>
Just plug the AC adapter into the connector marked "DC IN" located on the side of the
case.
<Precautions when not using this unit>
Even when the battery charge LED lamp is lit in green, a small amount of current flows into
the battery from the AC adapter. This will affect the battery service life, so unplug the AC
adapter from the AC outlet when not using the LUMITESTER.
<When a battery mark appears on the display panel>
The remaining battery power is low, so recharge the battery.
<CAUTION !! Always use the AC adapter supplied with the LUMITESTER.>
If an AC adapter other than supplied is used, the LUMITESTER may malfunction.
Ⓒ Kikkoman Corp. (04—1)
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5. Operating Procedure
5-1 Basic operation
This section explains basic operations of the LUMITESTER C-110.
The LUMITESTER C-110 can be operated in the following two modes for making
measurements.
• STANDARD mode
Measurement results are displayed in units of RLU (relative light unit), M, pg or CELL
which are selectable.
Measurement time is fixed to 10 seconds.
• ADVANCED mode
This mode allows start delay time setting, measurement time setting, and ON/OFF
switching of real-time count value display.
Measurement results are displayed only in RLU units.
5-1-1 STANDARD mode measurement
1) Turn the power on.
• Hold down the <POWER> key until the message at the
right appears. ＫＩＫＫＯＭＡＮ
ＬＵＭＩＴＥＳＴＥＲ
Ｖｅｒ．５．０
MEASURING
＃XXXX
１０
MENU
＊STANDARD MODE
ADVANCED MODE
READY
＃----
---，--- ＲＬＵ
• After 2 seconds, the current date, time, and remaining
battery level are displayed.
The remaining battery level is shown in 1 to 5 steps, with
step 5 indicating fully charged.
(The remaining battery level is not shown when the AC
adapter is connected.)
• The MAIN MENU screen then appears.
• Check that STANDARD mode shows an asterisk (*) and
press the <ENTER> key.
2) Prepare a tube containing the sample fluid.
• Open the measurement chamber cover with your finger.
• Put the sample tube into the measurement chamber.
• Press down the cover to close it.
3) Start measurement.
• Press the <ENTER> key.
• The message shown at the right appears and measurement
will start.
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• Wait until measurement is over (until the center digit counts down to “0”).
CAUTION ! Do not open the cover during measurement.
• After 10 seconds, the measurement results are displayed as
shown at the right. READY
＃XXXX
XXX，XXX ＲＬＵ
• When a printer is connected, the measurement results are
also printed out.
• In units other than "RLU", the measurement results below the BLANK value are displayed
as ND (Not Detected).
(The BLANK value is the amount of light emission obtained when a sample with zero
concentrations was measured.)
• If the message "Scale Over" appears, this means the light level emitted from the sample is
too high, exceeding the upper limit of the LUMITESTER measurement range.
<< Scale Over >>
The upper limit of LUMITESTER measurement range is 999,999 RLU (approximately
100,000 pg).
4) Take the sample tube out of the chamber.
• To prevent carrying the LUMITESTER with a tube containing a sample fluid still installed in
the measurement chamber, make it a habit to remove the sample tube after measurement.
• To continue measurement, repeat steps 2) to 4).
5) Turn the power off.
• Press the <POWER> key to turn the power off.
* If you attempt to turn off the power while a sample tube is still in the LUMITESTER, a
beep sounds, so open the cover and remove the sample tube.
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Data Memory
Measurement data is sequentially stored in memory as sample No. 0001 to No. 1000.
When data for sample No. 1000 is stored, the next data is stored in memory as sample No.
0001. All data stored in memory is backed up by battery, and will not be lost even when the
power is turned off.
* When measurement is started with MONITOR mode turned on, the data previously
stored in memory will be erased and the measurement data written in memory from No.
0001, so use caution.
To see the data previously measured, use the <SCROLL> keys.
<< Data Scroll >>
When scrolling with the < ∨> key, the display stops at the latest data.
When scrolling with the < ∧> key, the display does not stop but continues scrolling until
the < ∧> key is released.
When any sample number is displayed, pressing the <ENTER> key starts measurement
and the measurement data is stored in memory as the next sample number for the latest
data.
5-1-2 ADVANCED mode measurement
1) On the MAIN MENU screen, use the scroll keys to select the ADVANCED mode and
press the <ENTER> key.
*To set the measurement conditions, refer to "(10) Measurement time setting" and "(11)
MONITOR mode" in section 5-2, "Function Routines".
2) Prepare a tube containing the sample fluid.
• Open the measurement chamber cover with your finger.
• Put the sample tube into the measurement chamber.
• Press down the cover to close it
3) Start measurement.
• Press the <ENTER> key.
Measurement starts under the start delay and measurement time conditions which are set
by "MEASURING TIME" function routine.
MEASURING
＃XXXX
５
• Wait until measurement is over (until the center digit counts
down to “0”).
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When MONITOR mode is ON, the count value is displayed at specified time intervals (GATE
TIME).
• Measurement results are then displayed as shown at the
right.
In ADVANCED mode, the measurement data is always
displayed in RLU units regardless of which measurement
unit is selected.
READY
＃XXXX
XXX，XXX ＲＬＵ
4) Take the sample tube out of the chamber.
• To continue measurement, repeat steps 2) to 4).
5) Turn the power off.
• Press the <POWER> key to turn the power off.
* If you attempt to turn off the power while a sample tube is still in the LUMITESTER, a
beep sounds, so open the cover and remove the sample tube.
Ⓒ Kikkoman Corp. (04—1)
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5-2 Function routines
The LUMITESTER uses function routines to clear data or make various settings.
First of all, familiarize yourself with the basic operation flow for making condition settings
and selections.
LUMITESTER C-110 Menu List
STANDARD MODE
ADVANCED MODE
FUNCTION ROUTINE PRINT OUT DATA
CAL.CURVE
CLEAR ALL DATA
TRANS ALL DATA DATA
CAL.CURVE
SET-UP BEEP
LANGUAGE
DATE&TIME
COUNTRY
CHOOSE A UNIT RLU
M
pg
CELL
CHOOSE ATP/CELL A to E
INPUT ATP/CELL A to E
INPUT COEF.(pg)
INPUT CAL.CURVE
MEASURING TIME
MONITOR MODE
Press the <F> key to enter the function routine mode.
From the FUNCTION menu, select the desired function and press the <ENTER> key.
When the selection and setting are complete, the display returns to the MAIN MENU.
Pressing the <F> key on any screen returns to the MAIN MENU.
(In some functions, pressing the <ENTER> after pressing the <F> key determines the setting and
then returns to the MAIN MENU.)
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Each function is described below.
(1) Print [PRINT OUT]
Use this function to print out the stored measurement data or calibration curve.
Procedure
＊PRINT OUT
CLEAR ALL DATA
TRANS ALL DATA
SET-UP
PRINT OUT
＊ALL
SELECT DATE
SELECT NUMBER
Ｆ
ＥＮＴＥＲ
ＥＮＴＥＲ
ARE YOU SURE？
YES=ENTER
NO=FUNCTION
MAIN MENU
PRINTING DATA
STOP[ＦＵＮＣＴＩＯＮ]
ＥＮＴＥＲ
PRINT OUT
＊DATA
CAL.CURVE
ＥＮＴＥＲ
• In the PRINT OUT function, select the item you want to print.
"DATA"
Select this item to print measurement data.
"CAL.CURVE"
Select this item to print the currently set calibration curve.
• After selecting the item to print, press the <ENTER> key．
• When you selected "DATA", further select the data to print
from the following submenus.
"ALL"
Prints all data stored in memory.
・To abort printing, press the <F> key.
"SELECT DATE"
Prints all data of the specified measurement date.
・Press the <ENTER> key, and the cursor moves to the edit
position.
・Select the date using the scroll keys.
・After selecting the date, press the <F> key.
・When a conformation screen appears, press the <ENTER>
key to start printing.
・To abort printing, press the <F> key.
Ⓒ Kikkoman Corp. (04—1)
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"SELECT NUMBER"
Prints the data that is stored between the specified two data
numbers.
PRINT OUT
ALL
SELECT DATE
＊SELECT NUMBER
ＥＮＴＥＲ
NUMBER
#○○○○―#○○○○
MAIN MENU
ARE YOU SURE？
YES=ENTER
NO=FUNCTION
F
PRINT OUT
＊DATA
CAL.CURVE
PRINTING DATA
STOP[ＦＵＮＣＴＩＯＮ]
ＥＮＴＥＲ
・Press the <ENTER> key, and the cursor moves to the edit
position.
・Select the numbers using the scroll keys.
・After selecting the numbers, press the <F> key.
・ When a conformation screen appears, press the
<ENTER> key to start printing.
・To abort printing, press the <F> key.
* Input range
The right-hand data number must be equal to or larger than
the left-hand data number.
If the entry does not meet the above conditions or a data
number where no data is stored is entered, the cursor moves
back to that position.
CAUTION !
Be sure to turn off the power when connecting or
disconnecting a printer from the LIMITESTER.
Ⓒ Kikkoman Corp. (04—1)
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(2) Data clear [CLEAR ALL DATA]
Use this function to clear all data stored in memory.
Procedure
• In the CLEAR ALL DATA function, press the <ENTER>
key to clear the data.
PRINT OUT
＊CLEAR ALL DATA
TRANS ALL DATA
SET-UP
Ｆ
ＥＮＴＥＲ
ＥＮＴＥＲ
MAIN MENU
CLEAR ALL DATA
WITH ENTER KEY
• To cancel data clear, press the <F> key.
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