Knauer Azura P 2.1L User manual

Pump P 2.1L
Instructions
Document no. V6840
HPLCHPLC

Note: For your own safety, read the instructions and observe the war-
nings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V6840
Version number: 4.0
Release date: September 24, 2021
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library.
The printed versions of our instructions are printed according to
Blue Angel standards (www.blauer-engel.de/en/uz195).
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2021
All rights reserved.
AZURA® is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.
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AZURA® Pump P 2.1L Instructions V6840
Table of contents
1. General ..............................................................................1
1.1 About these instructions.........................................................1
1.2 Signal words ...................................................................1
1.3 Additional typographical conventions .............................................1
1.4 Legal information...............................................................1
1.4.1 Liability limitation......................................................1
1.4.2 Transport damage .....................................................2
1.4.3 Warranty conditions ...................................................2
1.4.4 Warranty seal .........................................................2
1.4.5 Declaration of conformity...............................................2
2. Basic safety instructions ................................................................3
2.1 Intended use...................................................................3
2.1.1 Operating ranges .....................................................3
2.1.2 Foreseeable misuse....................................................3
2.2 User qualification ...............................................................3
2.3 Operator responsibility..........................................................4
2.4 Personal safety equipment .......................................................4
2.5 Safety features on the device.....................................................4
2.6 Working with solvents ...........................................................5
2.6.1 General requirements..................................................5
2.6.2 Contamination by health-threatening solvents.............................5
2.6.3 Avoiding leakage......................................................5
2.7 Specific environments...........................................................6
2.7.1 Earthquake-endangered areas ..........................................6
2.7.2 Explosive environment .................................................6
2.7.3 Cooling room .........................................................6
2.7.4 Wet room ............................................................6
2.8 Maintenance, care and repair ....................................................6
2.9 Service request form and decontamination report ..................................7
3. Product information ...................................................................8
3.1 Features .......................................................................8
3.2 Scope of delivery ...............................................................8
3.3 Device overview................................................................8

AZURA® Pump P 2.1L Instructions V6840
Table of contents
3.4 Pump heads ..................................................................10
3.5 Eluents .......................................................................10
3.6 Meaning of the LEDs ...........................................................12
3.7 Symbols and signs.............................................................13
4. Installation and initial startup ..........................................................14
4.1 Unpacking....................................................................14
4.2 Ambient conditions ............................................................14
4.2.1 Operation site........................................................14
4.2.2 Ambient temperature .................................................14
4.2.3 Space requirements ..................................................15
4.3 Power supply .................................................................15
4.3.1 Power supply requirements ............................................15
4.3.2 Power supply cables and plugs.........................................15
4.4 Connecting the piston backflushing..............................................16
4.5 Eluent inlet ...................................................................17
4.5.1 Connecting the eluent inlet to the pump head ...........................18
4.5.2 Connecting the eluent line.............................................18
4.6 Changing the setup to LPG .....................................................19
4.6.1 Mounting the valve block..............................................20
4.6.2 Connecting the eluent line to the pump head ............................21
4.7 Priming the pump .............................................................22
4.8 Leak management .............................................................23
4.9 Control.......................................................................24
4.9.1 Setting up LAN.......................................................24
4.9.2 Configuring LAN settings..............................................25
4.9.3 Connecting devices with LAN ..........................................25
4.9.4 Configuring the router ................................................26
4.9.5 Integrating LAN into the company network ..............................26
4.9.6 Controlling several systems separately in LAN............................27
4.10 Setting IP addresses via software ................................................27
4.10.1 Mobile Control: Setting a static IP address ...............................27
4.10.2 Mobile Control: Setting a dynamic IP address via device name .............28
4.10.3 Mobile Control: Setting a dynamic IP address via device serial number ......29
4.10.4 Firmware Wizard: Setting a static IP address..............................30
4.10.5 Firmware Wizard: Setting a dynamic IP address...........................30
4.11 Remote control................................................................32

AZURA® Pump P 2.1L Instructions V6840
Table of contents
4.11.1 Connector assignment ................................................32
4.11.2 Connecting the pin header ............................................36
4.12 Analog control ................................................................37
5. Operation ...........................................................................38
5.1 Initial operation ...............................................................38
5.1.1 Pump head running-in ................................................38
5.2 Purging the pump .............................................................39
5.3 Switch-on.....................................................................40
5.4 Software control ...............................................................41
5.4.1 Controlling with chromatography software...............................41
5.4.2 Controlling with Mobile Control ........................................41
5.5 Switch-o.....................................................................42
6. Functionality tests ....................................................................43
6.1 Installation Qualification (IQ) ....................................................43
6.1.1 Definition of the installation qualification ................................43
6.1.2 Scope...............................................................43
6.1.3 Instructions ..........................................................43
6.1.4 About this document .................................................43
6.1.5 Device data..........................................................44
6.1.6 Customer data .......................................................44
6.1.7 Packing list, list of hardware and software ................................44
6.1.8 Initial startup .........................................................45
6.1.9 Comments/recommendations .........................................45
6.1.10 Change list ..........................................................45
6.1.11 Certificate ...........................................................45
6.2 Operation Qualification (OQ) ...................................................46
7. Troubleshooting......................................................................47
7.1 LAN..........................................................................47
7.2 Possible problems and solutions.................................................48
7.3 Error messages................................................................49
8. Maintenance and care ................................................................53
8.1 Maintenance contract ..........................................................53
8.2 Cleaning and caring for the device...............................................53
8.3 Pump head ...................................................................54
8.3.1 Screw fittings on the pump head .......................................54

AZURA® Pump P 2.1L Instructions V6840
Table of contents
8.3.2 Dismounting the pump head ..........................................54
8.3.3 Attaching capillaries to the pump head..................................56
8.4 Check valves ..................................................................57
8.4.1 Removing the check valves ............................................57
8.4.2 Cleaning the check valve ..............................................57
8.4.3 Installing the check valves .............................................58
9. Transport and storage.................................................................59
9.1 Taking the device out of operation...............................................59
9.2 Packing the device.............................................................59
9.3 Transporting the device ........................................................59
9.4 Storing the device .............................................................59
9.4.1 Storing the pump head................................................60
10. Disposal.............................................................................61
10.1 AVV-Marking Germany .........................................................61
10.2 WEEE registration number......................................................61
10.3 Eluents and other operating materials ............................................61
11. Technical data........................................................................62
11.1 Main features .................................................................62
11.2 Communication ...............................................................63
11.3 General ......................................................................63
12. Chemical compatibility of wetted materials..............................................64
12.1 General ......................................................................64
12.2 Plastics .......................................................................64
12.3 Non-metals ...................................................................66
12.4 Metals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
13. Repeat orders........................................................................68
13.1 Device and accessories.........................................................68
13.2 Pump heads with accessories ...................................................69
13.3 Documents ...................................................................69

AZURA® Pump P 2.1L Instructions V6840
1
1. General
1.1 About these instructions
These operating instructions enable the safe and ecient operation of
the device. The user must have carefully read and understood these
operating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see „2 Basic safety instructions“, p. 3). In addition to the
safety and warning instructions in these operating instructions, the local
accident prevention regulations and the national industrial safety regulati-
ons apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazar-
dous situation. If not avoided, it could result in
minor or moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
Note: Specific information are prefixed with the word „Note” and an
information icon.
Note: This is an example.
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions

AZURA® Pump P 2.1L Instructions V6840
2 General
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see „2.2 User quali-
fication“, p. 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and
unauthorized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is axed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician axes an orange seal in the same
place. If unauthorised persons tamper with the device or if the seal is
damaged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

AZURA® Pump P 2.1L Instructions V6840
3
2. Basic safety instructions
The device has been developed and constructed in such a way that
hazards arising from its intended use are largely excluded. Nevertheless,
the following safety instructions must be observed in order to exclude
residual hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used indoors for chromatographic
applications.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outdoors. Otherwise, the manufacturer does not guarantee
the functionality and safety of the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The users are qualified to handle the device if all of the following points
apply:
They have at least a basic knowledge of liquid chromatography.
They have knowledge about the properties of the used solvents and
their health risks.
They are trained for the special tasks and activities in the laboratory
and know the relevant standards and regulations.
Due to their technical training and experience, they can unders-
tand and carry out all the work described in the operating instruc-
tions on the instrument and recognize and avoid possible dangers
independently.
Their ability to react is not impaired by the consumption of drugs,
alcohol or medication.
They have participated in the installation of an instrument or training
by KNAUER or an authorized company.
If users do not meet these qualifications, they must inform their
supervisors.

AZURA® Pump P 2.1L Instructions V6840
4 Basic safety instructions
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use. It also bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations.
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules.
Ensure that all personnel who work with the device have read and
understood these operating instructions.
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective safety equipment which is specified in the
particular laboratory.
2.5 Safety features on the device
Power switch: Devices of the AZURA® L series may be switched o
using the power switch (toggle switch on the back side of housing) at
any time, this causes no damage to the device. To switch o devices of
the AZURA® S series, remove the plug from the power socket or use
the toggle switch of the power supply unit.
Front cover: Devices of the AZURA® L series have a front cover as a
splash protection for the user
Leak tray: Devices of the AZURA® L series have a leak tray on the front
side. The leak tray collects leaking solvents and protects components
from potential damage caused by discharging liquid.
Lamp: For the detectors AZURA DAD 2.1L, DAD 6.1L und MWD 2.1L,
the lamp switches o automatically when the cover is opened.

AZURA® Pump P 2.1L Instructions V6840
Basic safety instructions 5
2.6 Working with solvents
2.6.1 General requirements
The user is trained for handling dierent solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
„12 Chemical compatibility of wetted materials“, p. 64).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
Degas solvents before use and observe their purity.
2.6.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see „10 Disposal“, p. 61).
2.6.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch o the system. Only take the device into
operation if the cause of the leak has been resolved (see „8 Mainte-
nance and care“, p. 53).

AZURA® Pump P 2.1L Instructions V6840
6 Basic safety instructions
2.7 Specific environments
2 .7.1 Earthquake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is risk of injury due to falling devices
or loose parts.
2 .7. 2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2 .7. 3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2 .7. 4 Wet room
The device must not be operated in wet rooms.
2.8 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer‘s instructions on
caring for the columns (see www.knauer.net/columncare).
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER
Customer Support or any company authorized by KNAUER (see „1.4.1
Liability limitation“, p. 1).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting

AZURA® Pump P 2.1L Instructions V6840
Basic safety instructions 7
2.9 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest

AZURA® Pump P 2.1L Instructions V6840
8
3. Product information
Note: Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equipment of the
device could fail.
3.1 Features
The pump transports the fluid. By choosing the pump head accordingly,
it is possible to reach the maximum values (see „11.1 Main features“, p.
62).
The pump P2.1L oers the following features:
Self-priming pump
Prolonged operating time because of the automatic piston
backflushing
Leak management
KNAUER oers the following accessories:
Stainless-steel pump heads
Titanium pump heads for biocompatible applications
Display software Mobile Control
Heating/cooling elements for the pump head
Valve block for binary or ternary low pressure gradients
Pump head inlet for one eluent tubing
3.2 Scope of delivery
Note: Only use original parts and accessories made by the manufacturer
or a company authorized by the manufacturer.
AZURA® Pump P 2.1L
Power cable
Instructions DE/EN
Declarations of conformity
Accessories kit AZURA®
Accessories kit pump
3.3 Device overview
P2.1L is a self-priming pump with automatic piston backflushing that is
used in preparative HPLC systems.
Options

AZURA® Pump P 2.1L Instructions V6840
Product information 9
Legend
1
Status LEDs
2
Pump head
3
Pressure sensor
Fig. 1:
1
2
3
Pump P 2.1L front view
Legend
1
Left LED
2
Center LED
3
Right LED
4
Standby switch
Fig. 2:
1 2 3 4
LEDs and switch on the front of the device
Legend
1
Serial number
2
Pin header
3
LAN port
4
Interface for the
Technical Support
5
Main power con-
nection and power
switch
Fig. 3:
2
3 4 5
1
P2.1L rear view

AZURA® Pump P 2.1L Instructions V6840
10 Product information
As part of a HPLC system, the pump takes part in separating substan-
ce mixtures and in filtering substances. It can alternatively be used as
a single module. The pump transports the mobile phase within the
chromatography system. For transportation there are two operating
modes possible:
Standard mode: Fluid transportation with a flow rate of up to
1000ml/min
Dosing mode: Fluid transportation with a dose flow
In laboratories the device can be used in the following areas:
Separation of chiral substances
Separation of biomolecules
Separation of fine chemicals
Separation of active pharmaceutical ingredients (API)
3.4 Pump heads
The pump automatically recognizes the pump head by means of the RFID
chips. The pump head is equipped with an RFID chip. It is used to monitor
and save all important parameters and settings of the pump and pump
head.
Pump head for use in preparative applications:
Standard model, stainless steel
Pump heads with titanium or ceramic inlays for biocompatible applica-
tions 100ml, 250ml, 500ml, 1000ml
3.5 Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
Note: The solvent list contains the solvents KNAUER recommends
for use. If there is any doubt, contact the Technical Support of the
manufacturer.
Operating range
Location

AZURA® Pump P 2.1L Instructions V6840
Product information 11
Suitable solvents
Acetone at 4°C–25°C (39.2°F–77.0°F)¹
Acetonitrile²
Benzene
Dilute acetic acid (e.g. 10–50%) at 25°C/77.0°F
Dilute ammonia solution
Dilute sodium hydroxide (1M)
Ethanol¹
Ethyl acetate
Hexane/heptane at 4–25°C (39.2–77.0°F)¹
Isopropanol
Methanol
Phosphate buer solutions (0.5M)
Toluol
Water
1. Valid for the specified temperature range.
2. Not recommended in combination with PEEK small parts and PEEK capillary.
Less suitable solvents
Carbon dioxide (liquid 99.999%CO2)
Dilute phosphoriac acid
Dimethyl sulfoxide (DMSO)
Methylene chloride¹
Slightly volatile solvents
Tetrahydrofuran (THF)¹
1. Not recommended in combination with PEEK small parts and PEEK capillary.
Not suitable solvents
Chloroform
Concentrated mineral and organic acids
Concentrated bases
Halogenated hydrocarbons, e.g. Freon®
Perfluorinated solvents, e.g. Fluorinert® FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles

AZURA® Pump P 2.1L Instructions V6840
12 Product information
3.6 Meaning of the LEDs
There are three LEDs 1and a standby switch 2on the front of the
device.
The LEDs show dierent colors depending on the operating status.
Color Operating condition Operation
Left LED Flashes red Error error Check the system.
Shortly press the
switch to deac-
tivate the error
message.
Red Serious error Restart the device.
If the operating
condition does
not change, con-
tact the Technical
Support.
Green Program or sequen-
ce is running/was
loaded.
-
Center LED O Pump is ready and
does not convey
liquid.
-
Flashes green Equilibration Wait until the device
is ready.
Green Pump conveys liquid -
Right LED,
power status
Green Switched on -
Blue Standby Exit standby by
pressing the standby
switch.
Fig. 4: LED panel when the device is switched o
12

AZURA® Pump P 2.1L Instructions V6840
Product information 13
To activate the standby, keep the switch pressed for 5seconds.
Note: Malfunctioning system after repeated standby possible. After
repeatedly using the standby, switch o the power switch and back on
again, to reset the data storage.
3.7 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
High-voltage hazard
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
Obey maximum load for leak tray during trans-
portation, installation and operation.
A device marked with CE fulfills the product
specific requirements of European direc-
tives. This is confirmed in a Declaration of
Conformity.
The device complies with the product-specific
requirements of the United Kingdom.
Standby

AZURA® Pump P 2.1L Instructions V6840
14
4. Installation and initial startup
Before you determine the operation site, read the chapter “11. Technical
data” on page 62. There you will find all device-specific information on
power supply, ambient conditions and humidity.
Note: The intended use be ensured only if the requirements for ambient
conditions of the operating environment are met.
4.1 Unpacking
Process
1. Place the packaging in such a way that the lettering on the label is
in the correct position.
2. Check the packaging, the device and the accessories for transport
damage.
3. Check the scope of delivery. In the event of incomplete delivery,
contact Technical Support immediately.
4. When lifting, carrying or moving the device, grab the unit only from
below on the sides. Do not hold onto front cover or leak tray, as
these parts are loosely attached to the device.
Keep the included packing list for repeat orders.
Keep the original packaging for safe storage or transportation.
4.2 Ambient conditions
4.2.1 Operation site
Observe the following requirements for the operation site so that the
measurement results are not influenced:
Place on a firm, level and straight surface.
Protect against direct sunlight.
Do not expose to air drafts such as air conditioning systems.
Do not set up next to other machines that cause floor vibrations.
Keep from high frequency sources.
Ensure adequate ventilation (see “4.2.3 Space requirements”, p. 15).
Avoid temperature fluctuations (see „4.2.2 Ambient temperature“, p.
14).
Avoid electrostatic discharges at the pump head.
4.2.2 Ambient temperature
If the ambient temperature of the device is abruptly changed (e.g. when
it is installed in a cooling chamber), condensation will form inside the
device and may cause damage to the device. Allow the device to accli-
mate for 3 hours, before it is connected to the power supply and taken
into operation.
Procedure
Next steps