Knauer Azura P 6.1L User manual

Pump P 6.1L
User Manual
V6890

2
Note: For your own safety, read the manual and always observe the warnings and
safety information on the device and in the manual
Manuel en français: Si jamais vous préfériez un manuel en français pour ce produit, veuillez vous
contacter le support technique (Technical Support) par email ou par fax
avec le no. de série. Merci beaucoup.
Technical Support: Phone: [phone] (9-17h, Central European Time)
Fax: [phone]
E-Mail: [e-mail]
Languages: German, English
Publisher: KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
D-14163 Berlin
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [email protected]
Version information: Article number: V6890
Version number: 2.2
Last update: 2017-02-16
Original edition
The information in this document is subject
to change without prior notice.
For latest version of the manual, check our website:
http://www.knauer.net/en/downloads.html
Copyright: © KNAUER Wissenschaftliche Geräte GmbH 2017
All rights reserved.
® AZURA is a registered trademark of
KNAUER Wissenschaftliche Geräte GmbH.

P 6.1L user manual V6890
3TableofContents
Table of Contents
Product Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Operating Ranges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Views . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Front View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Rear View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Performance Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Unpacking the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Capillaries and Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Connecting the Solvent Bottles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Pre-Installed Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Integrating the Pump into an HPLC Flow System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Isocratic pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Binary pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the Solvent Selection Valve to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . 18
Quaternary Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Degasser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Connecting the Pump Head to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the 4-Channel Degasser to the Solvent . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Connecting the Valve Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting the Piston Backflushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Connecting the Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

P 6.1L user manual V6890
Table of Contents 4
Pin Header Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Remote Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Events Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Power Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Bleeding the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Switch-On . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operating with Chromatography Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Operating with Mobile Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Meaning of the LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Functional Tests IQ and OQ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Possible Problems and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Maintenance and Care. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Maintenance Intervals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Checking the Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Putting the Pump Out of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Disconnecting the Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Preparing the Pump Head for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Dismounting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Cleaning the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Installing the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Inline Filter on the Pressure Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Removing the Inline Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Inserting the New Inline Filter Cartridge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Replacing the Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Removing a Leak . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
P 6.1L General Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Communication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

P 6.1L user manual V6890
5TableofContents
Device Variants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
P 6.1L isocratic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
P 6.1L binary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
P 6.1L quaternary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Repeat Orders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Warranty Seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
HPLC Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

P 6.1L user manual V6890
Product Information 6
Product Information
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Description The pump P 6.1L is suitable for analytical and semi-preparative applications.
Operating Ranges
Operating ranges The device can be used in the following areas:
biochemical analysis
chemical analysis
food analysis
pharmaceutical analysis
environmental analysis
Device Variants
The P 6.1L is available in three different options:
Isocratic pump
Binary pump (HDG pump)
Quaternary Pump (NDG pump)
All variants are available as a standard version made of stainless steel or as a
bio-inert version made of ceramics with PEEK capillary, PEEK connectors,
and PEEK venting screw.
Views
Front View
Isocratic pump The isocratic pump is equipped with a pressure sensor with an integrated
inline filter and a venting screw..
Legend
1Pressure sensor
2Pump head
Fig. 1 Isocratic pump - front view
12

P 6.1L user manual V6890
7 Product Information
Binary pump The binary pump consists of two pump drives and a 2-channel degasser
with a Solvent Selection valve. A pressure sensor with an integrated inline fil-
ter and a venting screw are also part of the pump.
Quarternary pump The quaternary pump consists of a pump, a valve block and a 4-channelde-
gasser. A pressure sensor with an integrated inline filter, a venting screw,and
a mixer are also part of the pump.
Legend
1Pressure sensor
2Mixer
3Pump head
4Degasser
5Solvent Selection
valve
Fig. 2 Binary pump - front view
12345
Legend
1Pressure sensor
2mixing chamber
3Pump head
4Valve block
5Degasser
Fig. 3 Binary pump - front view
12 3 4 5

P 6.1L user manual V6890
Product Information 8
Rear View
On the back of pump, the connectors for LAN and power chord are locate-
das well as the serial number of the device an the power switch (on/off).
Service Interface Please note that the service interface is solely used for repair and mainte-
nance tasks performed by service technicians.
Performance Features
The pump is used to convey liquids, either in an HPLC system or as a stand-
alone device. Liquids are conveyed either with constant flow or with con-
stant pressure. The flow and pressure, which liquids can be conveyed with,
depend on the pump head.
Pump heads Pump heads for several areas of application are available for the pump P
6.1L:
Every pump head is equipped with an RFID chip. It is used to monitor and
save all important parameters and settings. The RFID technology offers the
following advantages:
Software automatically recognizes the parameters of the pump head
All service-relevant data of the pump head is stored
Mixer Volume of the mixer is 100 l. The mixer is exchangeable and can be
replaced by mixers with a volume of 50 µl or 200 µl .
The parameters necessary for the mixer are set via Mobile Control or via
chromatography software.
Legend
1serial number
2service interface
3Pin header
4LAN connector
5Power connection
and power switch
Fig. 4 Rear view (example)
123
45
Standard Bio-inert
Size 5 ml, 10 ml and 50 ml 10 ml and 50 ml
material Pump head with stainless
steel inlays
Pump head with ceramic
inlays
Operating
Range
HPLC
Dosing applications
with non-aggressive
media
Purification/FPLC
Ion chromatography

P 6.1L user manual V6890
9 Product Information
Piston backflushing The piston backflushing function automatically flushes the rear piston area
of the pump head upon switch-on and in continuous mode.
Upon switch-on: The rear piston area of the pump head is automatically
flushed for 15 seconds.
In continuous mode: The rear piston area of the pump head is flushed
automatically every 15 minutes, for 15 seconds.
PMax Mode This mode protects the column from excessive fluctuations in pressure. If
the maximum pressure is reached, the user can decide if the pump should
be switched off or continue running with constant pressure. You find a
detailed description on chromatography software in the corresponding user
manual.
GLP Data The Mobile Control and the different software products (ClarityChrom®or
OpenLAB®) can be used to display or read GLP data. You find a detailed
description on how to display or read out GLP data in the respective user
manuals.
Optional accessories The following optional accessories are available:
Pump head cooler for the pump head
Mobile Control
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
If there is any doubt, contact the Technical Support of the manufacturer.
Parameter stainless steel Bio-Inert
Size 50 µl, 100 µl, 200 µl 250 µl
Pressure 1000 bar 400 bar
Unsuitable Eluents
halogenated hydrocarbons, e.g. Freon®
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Less Suitable Eluents
diethylamine (0.1 %) (DEA)
dimethyl sulfoxide (DMSO)
slightly volatile eluents
methylene chloride1
tetrahydrofuran (THF)1
phosphoric dilute acid
triethylamine (0.1 %) (TEA)
trifluoroacetic acid (0.1 %) (TFA)
1. not recommended in combination with PEEK components or PEEK capillaries

P 6.1L user manual V6890
Product Information 10
Suitable Eluents
acetate buffer solutions
acetone at 4 °C-25 °C (39.2 °C-77.0 °F)1
acetonitrile2
benzene
chloroform
ethyl acetate
ethanol
formiate buffer solution
isopropanol
carbon dioxide (liquid 99.999 % CO2)
methanol
phosphate buffer solutions (0.5 M)
toluol
ammoniated dilute solution
acetic acid (0.1-1 %), at 25 °C
sodium hydroxide (1 M)
water
1. valid for the specified temperature range
2. not recommended in combination with PEEK components or PEEK capillaries
Unsuitable Eluents for degassers
Azides
benzene
Hydro fluoro solvents
halogenated hydrocarbons, e.g. Freon®
heptane
Hexafluoroisopropanol (HFIP)
hexane
Hydrofluoric acid solutions
Carbon dioxide (liquid 99.999 % CO2)
concentrated mineral and organic acids
concentrated bases
eluents containing particles
perfluorinated eluents, e.g. Fluorinert® FC-75, FC-40
perfluorinated polyether, e.g. Fomblin®
Hydrochloric acid
dilute sodium hydroxide (1 M)

P 6.1L user manual V6890
11 Scope of Delivery
Scope of Delivery
Note: Only use original parts and accessories made by KNAUER or a com-
pany authorized by KNAUER.
Pump P 6.1L
Power cable
AZURA accessories kit
P 6.1L accessories kit
User manual DE/EN
Installation Qualification
Safety for Users
Professional Group The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in this user manual under any circum-
stances. In this case, please contact your superior.
Safety Equipment When working with the device, take measures according to lab regulations
and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must be taken into
account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the user
manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of the
HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer’s instructions on caring for the colums.

P 6.1L user manual V6890
Safety for Users 12
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohib-
ited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids can-
not get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration. Ensure
that work areas are always well-ventilated! Wear protective gloves and
safety glasses when working on the device!
Where is use of the device
prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Technical
Support of KNAUER.
Secure decommissioning At any time, take the device completely out of operation by either switching
off the power switch or by pulling the power plug.
Opening the Device The device may be opened by the KNAUER Technical Support or any com-
pany authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal of
a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist
company or the operating company before they can be recommissioned,
repaired, sold, or disposed of. All materials or fluids used for decontamina-
tion must be collected separately and disposed of properly.

P 6.1L user manual V6890
13 Symbols and Signs
Decontamination Report Devices without a completed Decontamination Report will not be repaired.
If you would like to return a device to KNAUER, make sure to enclose a com-
pleted Decontamination Report with the device: http://www.knauer.net/
en/downloads/service.html
Symbols and Signs
The following symbols and signs can be found on the device, in the chroma-
tography software or in the user manual:
Installation
This chapter describes all preparatory steps prior to start-up. If you encoun-
ter difficulties during the installation, contact the Technical Support.
Transport
Carefully prepare the device for transport or storage. If you want to return
your device to KNAUER for repairs, enclose the Service Request Form which
can be downloaded from our website.
Device Data For a secure transport, note the weight and dimensions of the device (see
Technical Data).
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Lifting Clasp the device at its side panels and lift it out of the packaging. Do not
hold onto front cover or leak tray.
Symbol Meaning
Electric shock hazard
Note maximum weight-loading of the leak tray during
transport, installation and operation.
A device or system marked with CE fulfills the product
specific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally recog-
nized testing centers (NRTL). The certified device or sys-
tem has successfully passed the quality and security
tests.
contact Phone [phone]
Fax [phone]
Mail [email protected]

P 6.1L user manual V6890
Installation 14
Location Requirements
Only if the requirements for ambient conditions of the operating environ-
ment are met, can the intended use be ensured. Details on the operating
conditions can be found in the Technical Data section.
Device defect
The device overheats at exposure to sunlight and insufficient air circulation.
Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air cir-
culation.
Space Requirements At least 5 cm if another device is set up on one side
At least 10 cm if further devices are set up on both sides
At least 15 cm on the rear panel for the fan.
Leave the power plug on the rear of the device accessible to be able to
disconnect the device from the mains.
General requirements Position the device on a level and even surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts (A/C systems).
Do not set up the device in the vicinity of other machines that cause floor
vibrations.
Cooling room It is possible to operate the pump in a cooling compartment when the fol-
lowingrequirements are fulfilled:
The pump is switched on.
The pump is on standby modus.
Device defect
Changes of the environmental temperature cause condensation inside the
device.
Allow device to acclimate for 3 h, before connecting to power supply and
taking into operation.
Earthquake Areas If you are located in an earthquake area, use the bore holes 1in the side
panels to secure the device. The bore holes are located on either right or left
side panel.
Unpacking the Device
Prerequisite Check packaging for damage caused during transportation. If necessary, put
forward any claim for damages to the carrier.
Fig. 5 bore holes of device
1

P 6.1L user manual V6890
15 Installation
Tools Utility knife
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Process 1. Set up the package in such a way that you can read the label. Using the
utility knife, cut the adhesive tape and open the packaging.
2. Remove the foam insert. Take out the accessory kit and the manual.
3. Open the accessory kit and check the scope of delivery. In case any parts
are missing, contact the Technical Support.
4. Clasp the device from below, lift it out of the packaging and place it on
its feet. Do not hold onto the front cover.
5. Check the device for signs of damage that occurred during transport. In
case you notice any damage, contact the Technical Support.
6. Place the device in its site of operation and remove protective foil.
Next steps Store packaging and keep the included packing list for repeat orders.
Capillaries and Fittings
All tubing and capillary, which connect the components of the pump, are
pre-installed. Only the solvent bottles have to be connected and the pump
has to be integrated into the flow of the HPLC system.
Connecting the Solvent Bottles
To connect the solvent bottles, tubing with pre-installed solvent filters is
used. The tubing is connected to the device with flangeless fittings.
Note: Do not use any tools to tighten the fittings.
Pre-Installed Capillaries
Pre-installed stainless steel and PEEK capillaries are color-coded according to
their inner diameter.
Procedure Process Figure
1. Slide the flangeless fitting 1
over the tubing.
2. Slide the lock ring 2over the
tubing, cap with the sealing ring
3. Note the direction of the
lock ring, otherwise the sealing
ring could be damaged. The
thicker end of the lock ring must
point into the direction of the
fitting.
Fig. 6 Set-up of a flangeless fitting
3. Manually fasten the flangeless
fitting 4to the device.
Fig. 7 Fully assembled flangeless fit-
ting
123
4

P 6.1L user manual V6890
Installation 16
Note: PEEK capillaries are not suitable for use with pure acetonitrile. Aceto-
nitrile can cause capillaries to crack or rupture.
Integrating the Pump into an HPLC Flow System
The pump can be integrated into an HPLC flow system by connecting the
pressure sensor (isocratic version) or the mixer (binary or quaternary ver-
sion) and the HPLC system with capillary.
Note: To integrate the pump into a system, note the ambient conditions-
found in the sections Operating Environment and Technical Data as well
asthe ambient conditions of other devices to be integrated into that system.
Device defect
Damage to the pump head caused by overtightened capillary fittings. Note
the torque of the fittings.
Use 5 Nm torque for stainless-steel fittings.
Use 1 Nm torque for PEEK fittings.
Tools Torque wrench
Isocratic pump
The figure shows the installation plan for capillary and tubing on the isocra-
tic version of the pump. If you own a different version of the pump, see the
corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-
tle is used for the flushing solution.
Solvent flow path The pump head takes the liquid in from the bottle and conveys it to the
pressure sensor.
Color Material Inner Diameter
red marker stainless steel 0.1 mm
blue marker stainless steel 0.25 mm
black marker stainless steel 0.45 mm
red stripe PEEK 0.1 mm
blue stripes PEEK 0.25 mm
orange stripes PEEK 0.5 mm
Capillary Layout
Fig. 8 Installation plan for the isocratic pump

P 6.1L user manual V6890
17 Installation
Connecting the Pump Head to the Solvent
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
material flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Next Steps Integrate the pump into the HPLC flow system.
Binary pump
The figure shows the installation plan for capillary and tubing on the binary
version of the pump. If you own a different version of the pump, see the
corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-
tle is used for the flushing solution.
Procedure Process Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1on the bottom of the
pump head.
3. Tighten the fitting by hand.
Fig. 9 Solvent line on pump head
1
Capillary Layout
Fig. 10 Installation plan for the binary pump
1
23

P 6.1L user manual V6890
Installation 18
Every pump head can operate with two different solvents. Both solvents are
connected with the Solvent Selectionvalve 1. The solvents are conveyed
into one pump head each and combined in the pressure sensor. The pres-
sure sensor is connected to the mixer. The mixer is connected to the HPLC
system.
Connecting the Degasser
The degasser inlet 2is readily connected with the solvent selectionvalve 1.
The degasser outlet 3is readily connected with the pump heads (see Fig.
10).
Solvent flow path Both solvents are connected with the 2degasser inlet. The solvents are
conveyed from the degasser into the pump head. From the pump head it is
taken via the pressure sensor to the mixer. The mixer is connected to the
HPLC system.
Note: The described flow path is not suitable for Normal Phases.
Connecting the Pump Head to the Solvent
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
material flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Next Steps Integrate the pump into the HPLC flow system.
Connecting the Solvent Selection Valve to the Solvent
Note: The Solvent Selection valve is readily connected to the 2-channel
degasser.
The Solvent Selection valves allows selecting from two different solvents for
each solvent channel, without having to re-install the tubing. For both sol-
vent channels A and B, one of two solvents can be selected. Solvent A is
connected to inlets A1 and A2, solvent B is connected to inlets B1 and B2.
Procedure Process Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1on the bottom of the
pump head.
3. Tighten the fitting by hand.
Fig. 11 Solvent line on pump head
1

P 6.1L user manual V6890
19 Installation
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
material Flangeless fitting
Next Steps Integrate the pump into the HPLC flow system.
Quaternary Pump
The figure shows the installation plan for capillary and tubing on the quater-
nary version of the pump. If you own a different version of the pump, see
the corresponding chapter for installation instructions.
The gray lines represent the connection of the solvents to the pump and the
black lines represent the connection of the piston backflushing.
Piston backflushing The flushing solution is re-used. Since the flow path is circular, only one bot-
tle is used for the flushing solution.
Connecting the Degasser
The degasser outlet 2is readily connected to the valve block 1(see fig.
13).
Solvent flow path The four solvents are connected with the 3degasser inlet. The solvent mix-
ture is conveyed from the degasser via the valve block into the pump head.
From the pump head it is taken to the mixer. The mixer is connected to the
HPLC system.
Note: The described flow path is not suitable for Normal Phases.
Procedure Process Figure
1. Connect the tubing from the
four solvent bottles to the inlets
A1, A2 and B1, B2.
2. Seal inlets not in use with cap
fittings.
Fig. 12 Solvent Selection valve with
cap fitting
Capillary Layout
Fig. 13 Installation plan for the quaternary pump
12 3

P 6.1L user manual V6890
Installation 20
Connecting the Pump Head to the Solvent
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
Material Flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
Remove the cap fittings from the inlet and outlet of the pump head prior
to use.
Next Steps Integrate the pump into the HPLC flow system.
Connecting the 4-Channel Degasser to the Solvent
The 4-channel degasser contains four degassing chambers. Each degassing
chamber has an inlet and an outlet on the front of the pump.
Device defect
Very high pressures can damage the degasser membrane. The membrane
can withstand a maximum pressure of 7 bar.
Never connect the degasser to the pump outlet.
Prerequisites The device has been switched off.
The power plug has been pulled.
The front cover has been removed.
Material Flangeless fitting
Procedure Process Figure
1. Slide the flangeless fitting over
the tubing.
2. Insert the tubing into the free
inlet 1on the bottom of the
pump head.
3. Tighten the fitting by hand.
Fig. 14 Solvent line on pump head
1