Knauer BlueShadow 80P User manual

Pump 80P
Instructions
Document no. V7685
HPLCHPLC

Note: For your own safety, read the instructions and observe the war-
nings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V7685
Version number: 1.1
Release date: 2020/11/17
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library.
The printed versions of our instructions are printed according to Blue
Angel standards (www.blauer-engel.de/en/uz195).
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2020
All rights reserved.
Support:
Publisher:
Version information:
Sustainability:
Copyright:

BlueShadow Pump 80P Instructions V7685
Table of contents
Table of contents
1. General ..............................................................................1
1.1 About these instructions.........................................................1
1.2 Signal words ...................................................................1
1.3 Additional typographical conventions .............................................1
1.4 Legal information ...............................................................2
1.4.1 Liability limitation......................................................2
1.4.2 Transport damage .....................................................2
1.4.3 Warranty conditions ...................................................2
1.4.4 Warranty seal .........................................................2
1.4.5 Declaration of conformity...............................................2
2. Basic safety instructions ................................................................3
2.1 Intended use...................................................................3
2.1.1 Operating ranges .....................................................3
2.1.2 Foreseeable misuse....................................................3
2.2 User qualification ...............................................................3
2.3 Operator responsibility..........................................................4
2.4 Personal safety equipment .......................................................4
2.5 Working with solvents ...........................................................5
2.5.1 General requirements..................................................5
2.5.2 Contamination by health-threatening solvents.............................5
2.5.3 Avoiding leakage......................................................5
2.6 Specific environments...........................................................6
2.6.1 Earthqake-endangered areas ...........................................6
2.6.2 Explosive environment .................................................6
2.6.3 Cooling room .........................................................6
2.6.4 Wet room ............................................................6
2.7 Maintenance, care and repair ....................................................6
2.8 Service request form and decontamination report ..................................7
3. Product information ...................................................................8
3.1 Intended use...................................................................8
3.2 Performance features ...........................................................8
3.3 Device overview................................................................9
3.4 Pump heads ..................................................................10
3.5 Symbols and signs .............................................................11
3.6 Scope of delivery ..............................................................11

BlueShadow Pump 80P Instructions V7685
4. Installation...........................................................................12
4.1 Preparations ..................................................................12
4.1.1 Operating location ...................................................12
4.1.2 Power supply ........................................................13
4.1.3 Unpacking the device .................................................13
4.2 Connecting the piston backflushing..............................................14
4.3 Eluent inlet ...................................................................15
4.3.1 Connecting the eluent inlet to the pump head ...........................16
4.3.2 Connecting the eluent line.............................................17
4.4 Changing the setup to LPG .....................................................18
4.4.1 Valve block types .....................................................18
4.4.2 Connecting the LPG module ...........................................18
4.4.3 Controlling the LPG module ...........................................19
4.5 Control.......................................................................19
4.5.1 Pin header connectors ................................................20
4.5.2 Events connector .....................................................22
4.5.3 Connecting cables to the pin header....................................23
4.5.4 Connecting the device to a computer ...................................24
4.5.5 Configuring the LAN settings ..........................................25
4.5.6 Connect devices to LAN...............................................25
4.5.7 Configuring the router ................................................26
4.5.8 Integrating the LAN into the company network ...........................26
4.5.9 Controlling several systems separately in the LAN ........................26
4.5.10 Analog control .......................................................27
5. Operation ...........................................................................28
5.1 Switching on the pump.........................................................28
5.2 Configuring the LPG module....................................................28
5.3 Purging the pump .............................................................28
5.3.1 Piston backflushing ...................................................29
5.4 Switch on and self test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
5.5 Operating with LC display and keypad ...........................................30
5.5.1 LED status ...........................................................31
5.5.2 RFID icon............................................................31
5.5.3 Operating with the keypad ............................................32
5.6 Menu structure ................................................................33
5.6.1 Main menu ..........................................................33
5.6.2 Menu structure: overview..............................................34
5.6.3 Device setting parameters .............................................35
5.7 GLP menu ....................................................................39
5.8 Program menu ................................................................39
5.8.1 Create program ......................................................39

BlueShadow Pump 80P Instructions V7685
Table of contents
5.8.2 Run program.........................................................40
5.8.3 Change program .....................................................40
5.8.4 Delete program lines .................................................40
5.8.5 Delete a program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
5.8.6 Create a program with a starting time ...................................41
5.9 Link menu ....................................................................41
5.9.1 Create link...........................................................41
5.9.2 Run link .............................................................42
5.9.3 Delete link...........................................................42
6. Functionality tests ....................................................................43
6.1 Installation Qualification (IQ) ....................................................43
6.2 Operation Qualification (OQ) ...................................................43
7. Troubleshooting......................................................................44
7.1 LAN..........................................................................44
7.2 Possible problems and solutions.................................................45
7.3 System messages..............................................................46
8. Maintenance and care ................................................................50
8.1 Maintenance contract ..........................................................50
8.2 Cleaning and caring for the device...............................................50
8.3 Checking the fittings ...........................................................51
8.3.1 Torque values ........................................................51
8.3.2 Tighten fittings .......................................................51
8.4 Flushing the pump.............................................................51
8.5 Pump head ...................................................................52
8.5.1 Dismounting the pump head ..........................................52
8.5.2 Installing the pump head ..............................................53
8.6 Check valves ..................................................................54
8.6.1 Removing the check valves ............................................54
8.6.2 Cleaning the check valve ..............................................55
8.6.3 Installing the check valve ..............................................55
9. Transport and storage.................................................................56
9.1 Putting the pump out of operation ...............................................56
9.2 Packing the device.............................................................56
9.3 Transporting the device ........................................................56
9.4 Storing .......................................................................56
9.4.1 Storing the device ....................................................56
9.4.2 Storing the pump head................................................57

BlueShadow Pump 80P Instructions V7685
10. Disposal.............................................................................58
10.1 AVV-Marking Germany .........................................................58
10.2 WEEE registration number ......................................................58
10.3 Eluents and other operating materials ............................................58
11. Technical data........................................................................59
11.1 Solvent delivery ...............................................................59
11.2 Communication ...............................................................60
11.3 General ......................................................................60
12. Chemical compatibility of wetted materials..............................................62
12.1 General ......................................................................62
12.2 Plastics .......................................................................62
12.3 Non-metals ...................................................................64
12.4 Metals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
13. Repeat orders........................................................................66
13.1 Devices ......................................................................66
13.2 Spare parts ...................................................................66

1
BlueShadow Pump 80P Instructions V7685
General
1. General
1.1 About these instructions
These operating instructions enable the safe and efficient operation of
the device. The user must have carefully read and understood these ope-
rating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see chapter 2 on page 3). In addition to the safety and
warning instructions in these operating instructions, the local accident
prevention regulations and the national industrial safety regulations
apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word "Note” and an
information icon.
Note: This is an example.

2General
BlueShadow Pump 80P Instructions V7685
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see chapter 2.2 on
page 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unaut-
horized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is affixed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician affixes an orange seal in the same pla-
ce. If unauthorised persons tamper with the device or if the seal is dama-
ged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

3
BlueShadow Pump 80P Instructions V7685
Basic safety instructions
2. Basic safety instructions
The device has been developed and constructed in such a way that ha-
zards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used indoors for chromatographic
applications.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outdoors. Otherwise, the manufacturer does not guarantee
the functionality and safety of the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The user is qualified to handle the device if all of the following points
apply:
He has at least a basic knowledge of liquid chromatography.
He has knowledge about the properties of the used solvents and their
health risks.
He is trained for the special tasks and activities in the laboratory and
knows the relevant standards and regulations.
Due to his technical training and experience, he can understand and
carry out all the work described in the operating instructions on the
instrument and recognize and avoid possible dangers independently.
His ability to react is not impaired by the consumption of drugs,
alcohol or medication.
Participation in the installation of a device or a training by the company
KNAUER or an authorized company.
If the user does not meet these qualifications, he must inform his
supervisor.

4Basic safety instructions
BlueShadow Pump 80P Instructions V7685
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and un-
derstood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals).
Lab coat
Personalised protective safety equipment which is specified in the
particular laboratory.

5Basic safety instructions
BlueShadow Pump 80P Instructions V7685
2.5 Working with solvents
2.5.1 General requirements
The user is trained for handling different solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
chapter 12 on page 62).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
Degas solvents before use and observe their purity.
2.5.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see chapter 10 on page 58).
2.5.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch off the system. Only take the device into
operation if the cause of the leak has been resolved (see chapter 8 on
page 50).

6Basic safety instructions
BlueShadow Pump 80P Instructions V7685
2.6 Specific environments
2.6.1 Earthqake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is a risk of injury due to falling devices
or loose parts.
2.6.2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2.6.3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.6.4 Wet room
The device must not be operated in wet rooms.
2.7 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer's instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see chapter 1.4.1 on page 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting

7Basic safety instructions
BlueShadow Pump 80P Instructions V7685
2.8 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest

8Product information
BlueShadow Pump 80P Instructions V7685
3. Product information
Note: Only use the device for applications that fall within the range of
the intended use. Otherwise, the protective and safety equipment of the
device could fail.
Automatic piston backflushing increases the service life of the seals and
pistons, and removes salt and other substances from the area behind the
seals.
Isocratic operation:
Separation without gradients.
The solvent composition is constant during the analysis.
The solvent can be recycled.
Gradient operation:
LPG (Low Pressure Gradient): The gradient is formed on the low-pres-
sure side of the pump.
HPG (High Pressure Gradient): The gradient is formed on the
high-pressure side of the pump.
3.1 Intended use
The device or device system is suitable for high-pressure liquid chroma-
tography. It is suitable for laboratory use, for separating substance mix-
tures that can be dissolved in a solvent or solvent mixture.
Dosing pump
Separation of substances
Filtration of substances
3.2 Performance features
Dual-piston technology
Liquid transport with low pulsation, stable flow rate, and high flow
accuracy
Long service life
Internal controlling for programming binary to quaternary high-pres-
sure gradients
HPG system: up to four pumps can be combined
LPG system: Connection of a binary or ternary LPG valve block
Ultra-precise gradient mixtures throughout entire flow range
High physical and chemical stability
Direct pump control via analog and digital signals
Control with chromatography software
Operating modes

9Product information
BlueShadow Pump 80P Instructions V7685
3.3 Device overview
Legend
1
Display
2
Pressure sensor
3
Venting screw
4
Keypad
5
Tube connections for
the piston backflushing
6
Pump head
Fig. 1: Front view
Legend
1
Socket for the LPG valve
block
2
Events connector
3
Opening of the fan
4
Warranty seal
5
Power switch
6
Power supply
7
CE-mark
8
Remote connector
9
Interface RS-232
bl
LAN connection
bm Serial number
Fig. 2: Rear view
1235
5
6
4
1 2
bm
34
bl 9 8
5
6
7

10 Product information
BlueShadow Pump 80P Instructions V7685
3.4 Pump heads
Pump head for use in preparative applications:
Standard model, stainless steel
Titanium inlays for biocompatible applications: 100ml, 250ml, 500ml,
1000ml
For biocompatibility, pump heads with titanium inlays can be used. The
pump heads can be exchanged by the user.
The front of the pump head is labeled with the maximum pumping capac-
ity and the pump head inlays (SSt for stainless steel, Ti for titanium).
Fig. 3: Pump head 500 ml, stainless steel
The pump head is equipped with an RFID chip. It is used to monitor and
save all important parameters and settings of the pump and pump head.
The RFID technology oers the following advantages:
Automatic detection of all important pump head parameters by means
of radio signals (radio frequency identification), by the pump software
or the chromatography software:
— Pump head type
— Serial number and year of manufacture
— Number of cycles and operating times
— Limit values of the pump head parameters
All measuring data archived in accordance with GLP (Good Laboratory
Practice).
Transfer of all data to the software and pump display.
Designation
RFID detection

11Product information
BlueShadow Pump 80P Instructions V7685
3.5 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
Electric shock hazard
Electrostatic discharge hazard, damages to
system, device, or components can occur.
A device marked with CE fulfills the product spe-
cific requirements of European directives. This is
confirmed in a Declaration of Conformity.
Testing seals in Canada and the USA at nationally
recognized testing centers (NRTL). The certified
device or system has successfully passed the qual-
ity and security tests.
A warranty seal is affixed to some devices. For
more information see chap. 1.4.4 on p. 2.
3.6 Scope of delivery
Note: Only use original parts and accessories made by KNAUER or a
company authorized by KNAUER.
Power cable
BlueShadow Pump 80P with pump head
Accessories kit pump 80P
Accessories kit pump head
Applicable documents:
User manual
Installation Qualification
Declaration of Conformity

12 Installation
BlueShadow Pump 80P Instructions V7685
4. Installation
This chapter describes all preparatory steps prior to start-up.
4.1 Preparations
4.1.1 Operating location
The intended use be ensured only if the requirements for ambient con-
ditions of the operating environment are met. You will find the ambient
conditions under Technical Data.
Device defect
The device overheats at exposure to sunlight and insucient air circula-
tion. Device failures are very likely.
Set up the device in such a way that it is protected against exposure
to direct sunlight.
Leave room for air circulation: See paragraph „space requirements“.
At least 5cm, if there is another device on one side.
At least 10 cm, if there are devices set up on both sides.
At least 15 cm to the cooler fan on the rear.
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnect-
ed from the power supply.
Position the device on a level surface.
Protect the device against direct exposure to sunlight.
Set up the device at a location not exposed to air drafts such as air
conditioning systems.
Do not set up the device near to other machines that cause floor
vibrations.
Keep the the devices away from high frequency sources. High frequen-
cies may compromise measuring values.
Note: Make sure that the power plug on the rear of the device is always
accessible, so that the device can be disconnected from the power
supply.
Note: Avoid electrostatic discharges at the pump head.
Observe the following when moving the device from a cooling room to a
normal laboratory:
Switch on the device before moving and leave switched on for ½hour
to warm up.
As an alternative, allow the device to warm up in the laboratory for at
least 3hours and do not connect to the power supply during this dura-
tion to avoid damages by condensed water.
Space requirements
General purpose
Change of locaction

13Installation
BlueShadow Pump 80P Instructions V7685
Device defect
Changes of the environmental temperature cause condensation inside
the device.
Allow device to acclimate for 3h before connecting to power supply
and taking into operation.
4.1.2 Power supply
For power supply, use the supplied power cable and power adapter to
meet the specifications which are described in the chapter Technical
Data. But inspect the provided power cable beforehand to ensure that it
is approved for your country. Replace defective power cables only with
accessories from KNAUER. Detachable power supply cables are not
allowed to be replaced with other cable types.
The electrical power supply at the installation site must be connected
directly to the nearest main power line.
The power must be free from ripple, residual current, voltage peaks
and electromagnetic interference.
The connectors for the mains voltage are grounded accordingly.
The device receives sucient power with reserve capacity
The device is intended for use with AC power networks of 100-240 V.
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnect-
ed from the power supply.
4.1.3 Unpacking the device
Check the carton for damage caused during transportation.
Utility knife
Electronic defect
Electronic hazard when using an identically constructed power adapter
from another manufacturer.
Only use spare parts and accessories from KNAUER or a company
authorized by KNAUER.
Conditions
Power plug
Prerequisites
Tools
Bruising danger
Damage to the device by carrying or lifting it on protruding housing
parts. The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.

14 Installation
BlueShadow Pump 80P Instructions V7685
Process
1. Set-up the package in such a way that you can read the label.
2. Using the utility knife, cut the adhesive tape and open the
packaging.
3. Lift the foam padding. Take out the accessories kit and the manual.
4. Open the accessories kit and check the scope of delivery. In case
any parts are missing, contact the Technical Support.
5. Clasp the device from below, lift it out of the packaging and place
it on its feet. Do not hold onto the front cover.
6. Check the device for signs of damage that occurred during trans-
port. In case any parts are missing, contact the Technical Support.
7. Set up the device at the operation site. Lift the foam padding.
Store packaging and keep the included packing list for repeat orders.
4.2 Connecting the piston backflushing
The pump is equipped with automatic piston backflushing. It increases
the service life of the seals and pistons, and removes contaminants from
the area behind the seals.
The piston backflushing function automatically flushes the rear piston
area of the pump head upon switch-on and in continuous operation.
Upon switch-on: The rear piston area of the pump head is flushed
automatically for 15seconds.
In continuous mode: The rear piston area of the pump head is flushed
automatically every 30minutes, for 15 seconds.
Next steps
Functional principle