Knauer Azura P 2.1L User manual

Pump P 2.1L
User Manual
V6840
HPLC

P 2.1L user manual V6840, version 3.6
Table of Contents
Table of Contents
Note For your own safety, read the manual and always observe the warnings
and safety information on the device and in the manual!
Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Scope of Delivery. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Safety for Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Target group . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Safety equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
What must the user take into account? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Where is use of the device prohibited? . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Secure decommissioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Opening the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Warning notifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Decontamination Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Symbols and Signs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Contact Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Connecting the Piston Backflushing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Eluent Inlet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Connecting the Eluent Inlet to the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Connecting the Eluent Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Changing the Setup to LPG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Mounting the Valve Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Connecting the Eluent Line to the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Bleeding the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Leak Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Remote Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Remote Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Events Remote Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Connecting Cables to the Terminal Strip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

P 2.1L user manual V6840, version 3.6
4TableofContents
Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the LAN settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Connecting the cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Configuring the router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a company network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Controlling several systems separately in a LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Setting a Static IP Address . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Firmware Wizard: Setting an IP address to DHCP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Analog Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Running-in Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Switching on the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Possible Problems and Rectifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
System Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Contact with the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Cleaning and Caring for the Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Screw Fittings on the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Dismounting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Attaching Capillaries to the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Cleaning the Check Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Installing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Technical Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Device and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Pump Heads with Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Documents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Legal Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Transport damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Warranty conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Warranty seal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

P 2.1L user manual V6840, version 3.6
Intended Use 1
Intended Use
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the
device could fail.
Device Overview
P 2.1L is a self-priming pump with automatic piston backflushing that is
used in preparative HPLC systems.
Legend
1Status LEDs
2Pump head
3Pressure sensor
Fig. 1 Pump P 2.1L Front view
1
2
3
Legend
1Left LED
2Center LED
3Right LED
4Power switch
Fig. 2 LEDs and switch on the front of the device
123 4
Legend
1USB connector
Fig. 3 Connector Control Unit
6

P 2.1L user manual V6840, version 3.6
2 Intended Use
Operating range As part of a HPLC system, the pump takes part in separating substance
mixtures and in filtering substances. It can alternatively be used as a single
module. The pump transports the mobile phase within the chromatogra-
phy system. For transportation there are two operating modes possible:
Standard mode: Fluid transportation with a flow rate of up to 1000 ml/
min
Dosing mode Fluid transportation with a dose flow
Location In laboratories the device can be used in the following areas:
Separation of chiral substances
Separation of biomolecules
Separation of fine chemicals
Separation of active pharmaceutical ingredients (API)
Features
The pump transports the fluid. By choosing the pump head accordingly, it
is possible to reach the following maximum values:
Pressure up to 400 bar at a 100 ml/min flow rate
Flow rate of 1000 ml/min at a 50 bar pressure
P 2.1L offers the following features:
Self-priming pump
Prolonged operating time because of the automatic piston backflush-
ing
Leak management
Options KNAUER offers the following accessories:
Stainless-steel pump heads
titanium pump heads for biocompatible applications
Display control
Heating/cooling elements for the pump head
Valve block for binary or ternary low pressure gradients
Legend
1Serial number
2Pin header
3LAN port
4Interface for the
Technical Support
5Mains power con-
nection and power
switch
6Connector Control
Unit
Fig. 4 P 2.1L rear view
2
34 5
1

P 2.1L user manual V6840, version 3.6
Intended Use 3
Pump Heads
The pump automatically recognizes the pump head by means of the RFID
chips. The pump head is equipped with an RFID chip. It is used to monitor
and save all important parameters and settings of the pump and pump
head.
Pump head for use in preparative applications:
Standard model, stainless steel
Pump heads with titanium or ceramic inlays for biocompatible applica-
tions. 100 ml, 250 ml, 500 ml, 1000 ml
Eluents
Even small quantities of other substances, such as additives, modifiers, or
salts can influence the durability of the materials.
Note: The solvent list contains the solvents KNAUER recommends for use.
If there is any doubt, contact the Technical Support of the manufacturer.
Suitable solvents
Acetone at 4 °C–25 °C (39.2 °F–77.0 °F)1
Acetonitrile2
Benzene
Dilute acetic acid (e.g. 10–50 %) at 25 °C/77.0 °F
Dilute ammonia solution
Dilute sodium hydroxide (1 M)
Ethanol1
Ethyl acetate
Hexane/heptane at 4–25 °C (39.2–77.0 °F)1
Isopropanol
Methanol
Phosphate buffer solutions (0.5 M)
Toluol
Water
1. Valid for the specified temperature range.
2. Not recommended in combination with PEEK small parts and PEEK capillary.
Less suitable solvents
Carbon dioxide (liquid 99.999 % CO2)
Dilute phosphoric acid
Dimethyl sulfoxide (DMSO)
Methylene chloride1
Slightly volatile solvents
Tetrahydrofuran (THF)1
1. Not recommended in combination with PEEK small parts and PEEK capillary.

P 2.1L user manual V6840, version 3.6
4ScopeofDelivery
Scope of Delivery
Note: Only use original parts and accessories made by the manufacturer
or a company authorized by the manufacturer.
AZURA®Pump P 2.1L
24 V power supply power cable
User manual DE/EN
Declarations of conformity
Accessories kit AZURA®
Accessories kit pump
Safety for Users
Target group
This document address persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited
extent for use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the com-
pany KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may
not perform the work described in these instructions under any circum-
stances. In this case, please contact your superior.
Safety equipment
When working with the device, take measures according to lab regula-
tions and wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
What must the user take into account?
All safety instructions in this document
The environmental, installation, and connection specifications in this
document
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by
KNAUER
Good Laboratory Practice (GLP)
Not suitable solvents
Chloroform
Concentrated mineral and organic acids
Concentrated bases
Halogenated hydrocarbons, e.g. Freon®
Perfluorinated solvents, e.g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Solvents containing particles

P 2.1L user manual V6840, version 3.6
Safety for Users 5
Accident prevention regulations published by the accident insurance
companies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once the capillaries have been used, never re-use them in other areas
of the HPLC system.
Only use a given PEEK fitting for one specific port and never re-use it
for other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer’s instructions on caring for the
colums.
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries
can detach from their screw fittings and allow solvent to escape, it is
prohibited to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids
cannot get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point
higher than 150 °C under normal ambient conditions.
power strip: If several devices are connected to one power strip, always
consider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage
equals the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration.
Ensure that work areas are always well-ventilated! Wear protective
gloves and safety glasses when working on the device!
Where is use of the device prohibited?
Never use the system in potentially explosive atmospheres without appro-
priate protective equipment. For further information, contact the Techni-
cal Support of KNAUER.
Secure decommissioning
Take the device completely out of operation by either switching off the
power switch or by pulling the power plug.
Opening the device
The device may be opened by the KNAUER Technical Support or any
company authorized by KNAUER only.
Warning notifications
Possible dangers related to the device are divided into personal and
material damage in these instructions.
DANGER indicates a hazardous situation which, if not
avoided, will result in death or serious injury.
WARNING indicates a hazardous situation which, if not
avoided, could result in death or serious injury.
CAUTION indicates a hazardous situation which, if not
avoided, could result in minor or moderate injury.
NOTICE is used to address practices not related to phys-
ical injury.

P 2.1L user manual V6840, version 3.6
6 Symbols and Signs
Decontamination
Contamination of devices with toxic, infectious or radioactive substances
poses a hazard for all persons during operation, repair, sale, and disposal
of a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active
substances.
Before disposing of the device or sending it away for repair, you are
required to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a special-
ist company or the operating company before they can be recommis-
sioned, repaired, sold, or disposed of. All materials or fluids used for
decontamination must be collected separately and disposed of properly.
Decontamination Report
Devices without a completed Decontamination Report will not be
repaired. If you would like to return a device to KNAUER, make sure to
enclose a completed Decontamination Report with the device: http://
www.knauer.net/en/knowledge/downloads/service.html
Symbols and Signs
The following symbols and signs can be found on the device, in the chro-
matography software or in the user manual:
Installation
This chapter describes all preparatory steps prior to the start-up. If you
encounter difficulties during installation, contact the Technical Support.
Contact Data
Symbol Meaning
Electric shock hazard
Before opening the housing, switch the device
off and unplug from electrical power.
Electrostatic discharge hazard, damages to sys-
tem, device, or components can occur.
Heavy weight hazard, damage to leak tray can
occur.
CE mark A device or system marked with CE fulfills the
product specific requirements of European
directives. This is confirmed in a Declaration of
Conformity.
Phone [phone]
Fax [phone]
E-mail [email protected]

P 2.1L user manual V6840, version 3.6
Installation 7
Transport
Carefully prepare the device for transport or storage. If you want to return
your device to KNAUER for repairs, enclose the Service Request Form
which can be downloaded from our website.
Device Data For a secure transport, note the weight and dimensions of the device (see
Technical Data).
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Lifiting Clasp the device at its side panels and lift it out of the packaging. Do not
hold onto front cover or leak tray.
Location
Requirements
The location for the device must meet the following requirements:
Device defect
The device overheats at exposure to sunlight and insufficient air circula-
tion. Device failures are very likely.
Set up the device in such a way that it is protected against exposure to
direct sunlight.
Leave some space for air circulation: See space requirements.
Note: The leak sensor may malfunction it the device is placed on an
inclined surface. Use a level to check that the device is in an horizontal
position.
Power Supply
The device is intended for use with AC power networks of 100 – 240 V.
Power cable Only the supplied power cable is to be used to connect the device to the
mains supply. Replace defective power cables only with accessories from
KNAUER. Only use power cables with a permission for use from your
country.
Power plug Make sure that the power plug on rear of the device is always accessible,
so that the device can be disconnected from the power supply.
Unpacking
Store all packing material. Retain included packing list carefully for repeat
orders.
Tool Utility knife
position device or system on level surface
protect from heavy ventilation
Weight 19 kg
Dimensions (width
x height x depth)
361 × 201 × 523 mm
Power supply 100 – 240 V DC
Air humidity < 90 %, non-condensing
Temperature 4 – 40 °C/39.2 – 104 °F

P 2.1L user manual V6840, version 3.6
8Installation
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts.
The device may fall and thus cause injuries.
Lift the device only centrally on the side of the housing.
Procedure 1. Check for damage caused during transportation. In case you notice any
damage, contact the technical support and the forwarder company.
2. Setup the delivery so you are able to read the label. Using the utility
knife, cut the adhesive tape. Open the packaging.
3. Remove the foam insert. Take out the accessories kit and the manual.
4. Open the accessories kit and take out all accessories. Check the scope
of delivery. In case any parts are missing, contact the technical support.
5. Clasp the device at its side panels and lift it out of the packaging. Do
not hold onto front cover or leak tray.
6. Remove the foam inserts from the device.
7. Check for damage caused during transportation. In case you notice any
damage, contact the technical support.
8. Set-up the device in its location.
9. Remove the protective foil.
Connecting the Piston Backflushing
The piston backflushing removes salts and other substances from the area
behind the seals. To do this, connect a bottle with flushing solution to the
flush pump and to the pump head. The connection between pump head
and flush pump is preinstalled at the factory.
Functional principle The piston backflushing function automatically flushes the rear piston area
of the pump head upon switch-on and in continuous mode.
Upon switch-on: The rear piston area of the pump head is automatically
flushed for 30 seconds.
In continuous mode: The rear piston area of the pump head is flushed
automatically every 60 minutes, for 30 seconds.
Designation Inlet and outlet of the flush pump are located on the front of the device.
Flushing solution: These are suitable flushing solutions:
Water
Mixture of 80 % water and 20 % ethanol
Isopropanol
Note: KNAUER recommends the use of pure isopropanol as flushing solu-
tion for piston backflushing.
Device defect
Damage to the pump head caused by overtightened capillary fittings.
Note the torque of the fittings.
Note: PEEK fittings can withstand pressures up to 400 bar for 1/16" and
200 bar for 1/8".
Fig. 5 Inlet symbol Fig. 6 Outlet symbol

P 2.1L user manual V6840, version 3.6
Installation 9
Eluent Inlet
The eluent lines are connected by the eluent inlet to the pump head.
Before the eluent lines are connected, the eluent inlet must be attached to
the pump head.
Connecting the Eluent Inlet to the Pump Head
Connecting the Eluent Line
Prerequisite The eluent inlet has been connected.
Procedure Process Figure
1. Use the first hose to connect
the inlet 1of the flush pump to
the bottle containing the flush-
ing solution.
2. Lead a tube from the flush
pump's outlet 3 to the pump
head's inlet 4and fasten.
3. Use the second hose to con-
nect the pump head 2to the
bottle containing the flushing
solution. Fig. 7 Connectors of pump head
and flush pump
4
3
1
2
Legend
1Knurled-head screw
2Eluent inlet
3Olive-type tube fitting
Fig. 8 Eluent inlet for 100-500 ml pump heads
Fig. 9 Eluent inlet for 1000 ml pump head
1
2
3
Procedure Process Figure
1. With the knurled-head screw
2, screw the eluent inlet into
the inlet screw fitting 1of the
pump head.
2. Turn the eluent inlet until the
intake manifold input 3points
forward.
3. Tighten the knurled-head
screw. Fig. 10 Eluent inlet, variant 1
1
2
3

P 2.1L user manual V6840, version 3.6
10 Installation
Note: If connecting a pump head from 100 – 500 ml, make sure that the
tapered side of the cutting ring is pointed towards the fastening screw of
the Teflon tube.
Next steps Check whether the connectors and lines are tight.
Changing the Setup to LPG
By assembling a valve block, the setup of the system is changed to low
pressure gradient (LPG). The valve block is mounted to the front of the
pump. There are 2 types of valve blocks:
Types Ternary valve block for flow rates from 10 – 250 ml
Binary valve block for flow rates from 10 – 800 ml/min
Note: The manufacturer recommends to employ the binary LPG valve
block for flow rates in the range of 100 – 800 ml/min.
You can not use the front cover after having mounted the valve block.
Procedure Process Figure
Pump head:
100 ml
250 ml
500 ml
1. Push the Teflon hose 1
through the fastening screw 2
and the cutting ring 3.
2. Insert the hose end as far as
possible into the eluent inlet fit-
ting 4of the pump head.
3. Tighten the fastening screw by
hand.
Fig. 11 Connecting the eluent line
to the pump head
2
134
Procedure Process Figure
Pump head:
1000 ml
1. Push the Teflon hose directly
on the olive-type tube fitting
1.
2. Fasten the Teflon hose with
hose clamp.
Fig. 12 Eluent line on 1000 ml
pump head
1
Legend Legend
1Valve block
2Screw with seal ring
Fig. 13 Ternary valve block
1
2

P 2.1L user manual V6840, version 3.6
Installation 11
Mounting the Valve Block
Prerequisite The pump has been switched off.
The power plug has been pulled.
Tool Open-end wrench, size 17
Torque wrench
Note: Always place seals in pairs on both fastening screws.
Connecting the Eluent Line to the Pump Head
Procedure Process Figure
1. Unscrew the inlet 1from the
pump head.
Fig. 14 Inlet on the pump head
2. Place the seal rings 3on the
screws and valve block.
3. Using the torque wrench,
tighten the screws of the valve
block 2with a 7.5 Nm torque.
Fig. 15 Valve block
4. Insert the plug into the female
connector 4.
Fig. 16 Socket for the LPG valve
block
1
2
3
4
Procedure Process Figure
1. Push the Teflon hose 1
through the fastening screw 2
and the cutting ring 3.
2. Insert the hose end as far as
possible into the eluent inlet fit-
ting 4of the pump head.
Fig. 17 Cutting ring with Teflon
hose
12 3

P 2.1L user manual V6840, version 3.6
12 Installation
Next steps Connect the plug of the valve block.
Bleeding the Pump
Prerequisite The capillaries have been connected.
Tool Syringe
Column defect
Damage to the column due to bleeding.
Open the venting screw.
Remove the column.
Note: Before the pump can be used, it must be bleeded.
Result No air bubbles in the pump head and in the capillaries.
Leak Management
The leak management consists of the leak sensor and the drainage system
(funnels, hoses, nozzles). The drainage system ensures that escaping liq-
uids flow into a waste bottle. When leaks are registered by the leak sen-
sor, the LED flashes red. Both the device and the data acquisition via
chromatography software are stopped.
Prerequisite The front cover has been removed.
3. Tighten the fastening screw by
hand.
4. Plug a cap fitting into the
unused inlet.
Fig. 18 Inlets of the valve block
4
Procedure Process Figure
1. Open the venting screw 1of
the pressure sensor.
2. With the syringe, extract fluid
through the bleed port 2.
3. If the extracted fluid flows con-
tinuously, stop suction and
close the venting screw.
Fig. 19 Venting screw of the pres-
sure sensor
1
2
Process Figure
1. Carefully push the funnel 1
into the center opening of the
capillary guide 2.
Fig. 20 Funnel and capillary guide
12

P 2.1L user manual V6840, version 3.6
Installation 13
Next step Attach the front cover.
Remote Control
Remote control means to operate the pump via the terminal strip. The
connector is located on the rear side.
2. Push the long ending of the first
nozzle 4into the hose 3.
Fig. 21 Hose and nozzle
3. Connect the nozzle and the fun-
nel.
4. Push the other end of the hose
onto the nozzle 5of the leak
tray.
Fig. 22 Hose connected to device
5. For the bottom device, push
the short end of the nozzle 6
into the opening in the collec-
tion point of the leak tray.
6. Connect the hose to the nozzle
and lead the second ending to
the waste bottle.
7. Place the waste bottle below
the bottom device.
Fig. 23 Leak tray with nozzle
Process Figure
3
4
5
6
Legend
1Terminal strip
Fig. 24 P 2.1L rear view
1

P 2.1L user manual V6840, version 3.6
14 Installation
Remote Terminal Strip
For receiving start, control, and error signals from external devices
For sending start, control and error signals to external devices
Events Remote Connector
For test purposes or in some other cases, it can make sense to manually
enter these signals:
Sending control signals (Events) to external devices
Opening and closing contacts
Legend
1Display
2 Events Remote Con-
nector
Fig. 25 Remote terminal strip
1
2
Signal Explanation
ANALOG:
OUT
Analog output signal. Either outputs the measured
system pressure or a control voltage for Pump B. The
output range can be set to values of max. 1, 2, 5 and
10 V.
ANALOG: IN Analog input signal for
controlling the flow rate, e.g.:
1 V for 1 ml/min in the case of the 10 ml pump head
1 V for 5 ml/min in the case of the 50 ml pump
head.
START: OUT Output is active for 500 ms when the pump starts.
START: IN Activated by a voltage of 0 V against GND.
ERROR: OUT Output is active until the Error condition has been
eliminated.
ERROR: IN A voltage of 0 V against GND stops the pump.

P 2.1L user manual V6840, version 3.6
Installation 15
Activating 500 ms pulses
Legend
1Display
2 Events Remote Con-
nector
Fig. 26 Relation between display and Events terminal strip
1
2
Assignment Connection Function
1TTL TTL-compatible output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms
2TTL TTL-compatible output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms
3OC TTL output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms
GND Reference point of the voltage at the signal inputs.
4TTL TTL-compatible output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms
5TTL TTL-compatible output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms

P 2.1L user manual V6840, version 3.6
16 Installation
6OC TTL output
Levels:
passive 0 V
active 5 V
Pulse:
5 V for at least 1000 ms
GND Reference point of the voltage at the signal inputs.
Relay contact
The contact is on a floating basis. Its setting depends
on the settings in the Control Unit or software.
Steady-rate signal:
passive = open relay contact
active = closed relay contact
Pulse:
Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/ 24 V
DC
Relay contact
The contact is on a floating basis. Its setting depends
on the settings in the Control Unit or software.
Steady-rate signal:
passive = open relay contact
active = closed relay contact
Pulse:
Closed relay contact for at least 1000 ms
Permissible load of the relay contact: 1 A/ 24 V
DC
Analog GND Reference point of the voltage at the signal inputs.
Analog out Voltage range 0 – 5 V, scalable
Analog in Voltage range 0 – 10 V
10 V according to maximum flow rate
Start GND Reference point of the voltage at the signal inputs.
Start OUT TTL output
Levels:
passive 5 V
active 0 V
Start IN TTL input
Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground) from
an external device, the device starts. If controlled with
software, an electronic trigger is send through the
LAN.
Assignment Connection Function

P 2.1L user manual V6840, version 3.6
Installation 17
Connecting Cables to the Terminal Strip
To control one device through another, you use the multi-pin connector.
To use remote control, you have to connect cables to the terminal strip
(both included with delivery). The single ports are used to exchange con-
trol signals.
Prerequisite The device has been turned off.
The power plug has been pulled.
Tools Operating tool
Electronic defect
Connecting cables to the multi-pin connector of a switched on device
causes a short circuit.
Turn off the device before connecting cables.
Pull the power plug.
Electronic defect
Electrostatic discharge can destroy the electronics.
Wear a protective bracelet against electrostatic discharge and ground.
Process 1. Push the operating tool 3into an
upper small opening on the front
of the terminal strip 1.
2. Lead the cable into the opening
2below the inserted operating
tool.
3. Remove the operating tool.
Error OUT TTL output
Levels:
passive 5 V
active 0 V
Error IN TTL input
Low active
Secure switching threshold at least 10 mA
After receiving a signal (short-circuit to ground) from
an external device, an error message appears and the
device stops.
Error GND Reference point of the voltage at the signal inputs.
+24V Event-controlled switching of 24 V
against GND
Protection: 24 V – 200 mA
+5V Provides a voltage of 5 V with respect to GND. This
makes it possible to supply a consumer that is
switched by an EVENT.
Protection: 5 V – 50 mA
GND Reference point of the voltage at the signal inputs.
Assignment Connection Function
2
3
1