Knauer AZURA P 2.1S User manual

Pump P 2.1S/P 4.1S
User Manual
V6870
HPLC

Note: The contents of this user manual apply for the BlueShadow product as
well. Please submit any request on article numbers for BlueShadow.

Table of Contents 3
P 2.1S/P 4.1S user manual V6870, version 3.1
Table of Contents
Note: For your own safety, read the manual and always observe the warnings
and safety information on the device and in the
manual!
Intended Use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Device Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Eluents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Scope of Delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Symbols and Signs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Unpacking and Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Location Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Connecting the Eluent Line to the Pump Head . . . . . . . . . . . . . . . . . . . . 14
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Pin Header for Remote Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Computer Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Configuring the LAN Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Connecting the Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Configuring the Router . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Integrating the LAN into a Company Network . . . . . . . . . . . . . . . . . . 19
Controlling Several Systems Separately in a LAN . . . . . . . . . . . . . . . . 19
Ground . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Switching on the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Selecting the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Flushing the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4TableofContents
P 2.1S/P 4.1S user manual V6870, version 3.1
Switching Off the Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Functionality Tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
LAN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Error List and Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Maintenance and Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Contacting the Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Maintenance Contract . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Removing the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Installing the Pump Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Removing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Cleaning the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Installing the Check Valves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Technical Data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Accessories and Spare Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Device and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Device Variations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Available Pump Heads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Legal Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Transport Damage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Warranty Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Declaration of Conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Abbreviations and Terminology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Intended Use 5
P 2.1S/P 4.1S user manual V6870, version 3.1
Intended Use
Note: Only use the device for applications that fall within the range of the
intended use. Otherwise, the protective and safety equipment of the device
could fail.
Device Overview
The HPLC pumps P 4.1S/ P 2.1S with pump heads can be used as feed pumps
or dosing pumps in analytical or preparative applications. Pumps traP 2.1S/P
4.1S user manual V6870, version 3.1nsport solvents or dissolved samples
through the HPLC system.
Fig. 1 P 2.1S with 10 ml pump head Fig. 2 P 4.1S with 10 ml pump head
Legend
1 Display
2 Keypad
3 Pump head
4 Pressure sensor
Fig. 3 Pump, rear view
12
3
4
1
2
3
4
6
5
7
8

6 Intended Use
P 2.1S/P 4.1S user manual V6870, version 3.1
External devices like computers, fraction collectors, etc. can be connected in 3
different ways:
Connected to LAN within a network
Connected to RS232, alternately to LAN connection
Control with pin header
Location
In laboratories the device can be used in the following areas:
Biochemistry analyses
Food analyses
Pharmaceutical analyses
Environmental analyses
Chemical analyses
Dosing applications
Features
Analytical pump head with a flow rate range from 0.001 – 9.999 ml/min and
a pressure of up to 400 bar
Semi-preparative pump head with a flow rate range from 0.01 – 50 ml/min
and a pressure of up to 150 bar
Dual-piston technology for constant flow rates
Setting a limit for minimum and maximum pressure to protect the columns
and to avoid a dry run of the pump (only P 4.1S)
Emergency stop, independent from control with chromatography software
The pump can be controlled with the keypad in standalone mode or with the
chromatography software.
The pump heads can be easily removed and replaced via four front-accessible
screws by the user.
Unlike the pump P 2.1S, pump P 4.1S is equipped with a pressure sensor.
Features
Liquid transport with stable flow rate and high flow accuracy
Long service life
Pump heads completely made of stainless steel or with Hastelloy-C or
ceramic inlays
Legend
1 Opening of the fan
2 CE mark
3 Serial number
4 RS-232 port
5 LAN port
6 Pin header for remote control
7 Power connection - bushing
8 Hole for the ground connection

Intended Use 7
P 2.1S/P 4.1S user manual V6870, version 3.1
Piston backflushing
High physical and chemical stability
Flexible control with LAN connection, RS-232 interface, and analog control
signals
Control with chromatography software
Options
A pump in combination with another pump can be used to set up a binary high
pressure gradient system.
Pump Heads
Pump heads for use in analytical applications:
Stainless steel with stainless steel inlays for standard applications
Stainless steel with ceramics inlays for biocompatibility
Stainless steel with Hastelloy-C inlays for aggressive media
different pump head sizes: 10 ml or 50 ml
The front of the pump head is labeled with the specifications for the maximum
pumping capacity (10 ml or 50 ml). Pump heads with inlays carry additional
material labels (SST for stainless steel, C for ceramics, HC for Hastelloy-C).
Fig. 4 Labeling on the pump heads
Legend
1 flow rate performance
2 material
2
1

8 Intended Use
P 2.1S/P 4.1S user manual V6870, version 3.1
Eluents
Even small quantities of other substances, such as additives, modifiers, or salts
can influence the durability of the materials.
Note: The list of selected solvents was compiled based on research in the perti-
nent literature and is only a recommendation. If there is any doubt, contact the
Technical Support of the manufacturer.
Not suitable eluents
Halogenated hydrocarbons, e.g. Freon®
Concentrated mineral and organic acids
Concentrated bases
Eluents containing particles
Perfluorinated eluents, e. g. Fluorinert®FC-75, FC-40
Perfluorinated polyether, e.g. Fomblin®
Less suitable eluents
Dimethyl sulfoxide (DMSO)
Slightly volatile eluents
Methylene chloride
Tetrahydrofuran (THF)
Dilute phosphoric acid
Suitable eluents
Acetone at 4°-25° C (39.2°-77.0° F)1
Acetonitrile
Benzene
Chloroform
Ethyl acetate
Ethanol
Hexane/heptane at 4°-25° C (39.2°-77.0° F)1
Isopropanol
Carbon dioxide (liquid 99.999% CO2)
MethanolPhosphate buffer solutions (0.5 M)
Toluol
Dilute ammonia solution

Scope of Delivery 9
P 2.1S/P 4.1S user manual V6870, version 3.1
Scope of Delivery
Note: Only use original parts and accessories made by KNAUER or a company
authorized by KNAUER.
Pump P 2.1S/P 4.1S
User manual
Power adapter 24 V
Installation Qualification Document
Accessories Kit Pump
Accessories Kit AZURA
Safety
Professional Group
The user manual addresses persons who are qualified as chemical laboratory
technicians or have completed comparable vocational training.
The following knowledge is required:
Fundamental knowledge of liquid chromatography
Knowledge regarding substances that are suitable only to a limited extent for
use in liquid chromatography
Knowledge regarding the health risks of chemicals
Participation during an installation of a device or a training by the company
KNAUER or an authorized company.
If you do not belong to this or a comparable professional group, you may not
perform the work described in this user manual under any circumstances. In this
case, please contact your superior.
Safety Equipment
When working with the device, take measures according to lab regulations and
wear protective clothing:
Safety glasses with side protection
Protective gloves
Lab coat
Dilute acetic acid
(10-50%), at 25° C/77.0° F
Dilute sodium hydroxide (1M)
Water
1. valid for the specified temperature range
Suitable eluents

10 Safety
P 2.1S/P 4.1S user manual V6870, version 3.1
What must be taken into account?
All safety instructions in the user manual
The environmental, installation, and connection specifications in the user
manual
National and international regulations pertaining to laboratory work
Original spare parts, tools, and solvents made or recommended by KNAUER
Good Laboratory Practice (GLP)
Accident prevention regulations published by the accident insurance compa-
nies for laboratory work
Filtration of substances under analysis
Use of inline filters
Once they have been used, never re-use capillaries in other areas of the HPLC
system.
Only use a given PEEK fitting for one specific port and never re-use it for
other ports. Always install new PEEK fittings on each separate port.
Follow KNAUER or manufacturer's instructions on caring for the columns
More safety-relevant information is listed below:
flammability: Organic solvents are highly flammable. Since capillaries can
detach from their screw fittings and allow solvent to escape, it is prohibited
to have any open flames near the analytical system.
solvent tray: Risk of electrical shock or short circuit if liquids get into the
device's interior. For this reason, place all bottles in a solvent tray.
solvent lines: Install capillaries and tubing in such a way that liquids cannot
get into the interior in case of a leak.
leaks: Regularly check if any system components are leaking.
power cable: Defective power cables are not to be used to connect the
device and the power supply system.
self-ignition point: Only use eluents that have a self-ignition point higher
than 150 °C under normal ambient conditions.
power strip: If several devices are connected to one power strip, always con-
sider the maximum power consumption of each device.
power supply: Only connect devices to voltage sources, whose voltage equals
the device's voltage.
toxicity: Organic eluents are toxic above a certain concentration. Ensure that
work areas are always well-ventilated! Wear protective gloves and safety
glasses when working on the device!
Where is use of the device prohibited?
Never use the system in potentially explosive atmospheres without appropriate
protective equipment. For further information, contact the Technical Support of
KNAUER.

Safety 11
P 2.1S/P 4.1S user manual V6870, version 3.1
Secure decommissioning
At any time, take the device completely out of operation by either switching off
the power switch or by pulling the power plug.
Opening the Device
The device may be opened by the KNAUER Technical Support or any company
authorized by KNAUER only.
Signal Words
Possible dangers related to the device are divided into personal and material
damage in this user manual.
Lethal injuries will occur.
Serious or moderate injuries can occur.
Minor injuries can occur.
Device defects can occur.
Decontamination
Contamination of devices with toxic, infectious or radioactive substances poses
a hazard for all persons during operation, repair, sale, and disposal of a device.
Life-threatening injuries
Health danger if getting in contact with toxic, infectious or radio-active sub-
stances.
Before disposing of the device or sending it away for repair, you are required
to decontaminate the device in a technically correct manner.
All contaminated devices must be properly decontaminated by a specialist com-
pany or the operating company before they can be recommissioned, repaired,
sold, or disposed of. All materials or fluids used for decontamination must be
collected separately and disposed of properly.
Decontaminatin Report
Devices without a completed Decontamination Report will not be repaired. If
you would like to return a device to KNAUER, make sure to enclose a completed
Decontamination Report with the device: http://www.knauer.net/en/down-
loads/service.html

12 Symbols and Signs
P 2.1S/P 4.1S user manual V6870, version 3.1
Symbols and Signs
The following symbols and signs can be found on the device, in the chromatog-
raphy software or in the user manual:
Unpacking and Setup
Contacting the Technical Support
You have various options to contact the technical support:
Phone:[phone]
Fax:[phone]
E-mail:[email protected]
Location Requirements
Requirements
The location for the device must meet the following requirements:
Device defect
The device overheats at exposure to sunlight and insufficient air circulation.
Device failures are very likely.
Set up the device in such a way that it is protected against exposure to direct
sunlight.
Keep at least 15 cm clear at the rear and 5–10 cm at each side for air circula-
tion.
Symbol Meaning
Electric shock hazard
Electrostatic discharge hazard, damages to system,
device, or components can occur.
A device or system marked with CE fulfills the product
specific requirements of European directives. This is con-
firmed in a Declaration of Conformity.

Unpacking and Setup 13
P 2.1S/P 4.1S user manual V6870, version 3.1
Power supply
The device is intended for use with AC power networks of 100 – 240 V.
Power cable
Only the supplied power cable is to be used to connect the device to the mains
supply. Replace defective power cables only with accessories from KANUER.
Only use power cables with a permission for use from your country.
Power plug
Make sure that the power plug on rear of the device is always accessible, so that
the device can be disconnected from the power supply.
Unpacking
Store all packing material. Retain included packing list carefully for repeat
orders.
Bruising danger
Damage to the device by carrying or lifting it on protruding housing parts. The
device may fall and thus cause injuries.
Lift the device on the side of the housing only.
Process:
1. Setup the delivery so you are able to read the label. Using the utility knife,
cut the adhesive tape. Open the delivery.
2. Remove the foam insert. Take out the accessories kit and the manual.
Weight 1.5 kg
Dimensions 105 × 100 × 185 mm (Width × Height × Depth)
Power supply 24 V DC
Air humidity < 90 %
Temperature 4 – 40 °C (39.2 – 104 °F)
Space requirements Side clearance to other devices:
If there is a device on one side, minimum
clearance of 5 cm.
If there are devices on both sides, minimum
clearance of 10 cm
At least 30 cm gap to the fan on the rear of the
device.

14 Unpacking and Setup
P 2.1S/P 4.1S user manual V6870, version 3.1
3. Open the accessories kit and take out all accessories. In case any parts are
missing, contact the technical support. Grip the device at its side panels and
lift it out of the packaging.
4. Remove the foam inserts from the device. Pull off the antistatic bag, if neces-
sary.
5. Check for damages caused during transportation. In case you notice any
damage, contact the technical support.
6. Set-up the device in its location.
7. Check the scope of delivery
8. Remove the protective foil.
Result: The device is complete and ready for use.
Next steps: Connect the device to power supply to prepare the initial startup.
Connecting the Eluent Line to the Pump Head
Prerequisite:
The device has been switched off.
The power plug has been pulled.
Tools: Flangeless fitting
Device defect
The pump head can get damaged when the inlet and outlet are blocked.
Remove the cap fittings from the inlet and outlet of the pump head prior to
use.
Process Figure
1. Slide the flangeless fitting onto the
tubing.
2. Insert the tubing into the free inlet
1on the bottom of the pump
head.
3. Tighten the fitting by hand.
Fig. 5 Solvent line on the pump head
1

Unpacking and Setup 15
P 2.1S/P 4.1S user manual V6870, version 3.1
Next steps: Integrate the pump into the HPLC flow system.
Control
Pin Header for Remote Control
Fig. 6 Pump, rear view
Legend
1 CE mark
2 Opening of the fan
3 Serial number
4 RS-232 port
5 LAN port
6 Pin header for remote control
7 Power connection - bushing
8 Warning 1
9 Hole for the ground connection
Contact data Explanation
GROUND Reference point of the voltage at the signal inputs.
START IN TTL-compatible input
min. 10 mA
Low active
After receiving a signal from an external device, the
device starts. If controlled with software, an electronic
trigger is send through the LAN.
123
4
6
5
7
89

16 Unpacking and Setup
P 2.1S/P 4.1S user manual V6870, version 3.1
Connecting the Pin Header
To control one device through another, you use the multi-pin connec-
tor. To use remote control, you have to connect cables to the terminal
strip (everything comes included with delivery). The single ports are
used to exchange control signals.
Prerequisite:
The device has been turned off.
The power plug has been pulled.
Tools: Depressor tool
Electronic defect
Connecting cables to the multi-pin connector of a switched on device causes a
short circuit.
Turn off the device before connecting cables.
Pull the power plug.
Electronic defect
Electrostatic discharge can destroy the electronics.
Wear a protective bracelet against electrostatic discharge and ground.
ERROR IN/OUT TTL-compatible input
min. 10 mA
Low active
After receiving a signal (e. g. short-circuit to ground)
from an external device, an error message appears and
the device stops.
ANALOG IN Flow rate is controlled through external control voltage
(0–10 V).
ANALOG OUT Analog output signal for reproducing the measured sys-
tem pressure (0–1 V).
Contact data Explanation

Unpacking and Setup 17
P 2.1S/P 4.1S user manual V6870, version 3.1
Next steps: Finish the installation and perform the initial startup.
Computer Control
This section describes how to set up an HPLC system in a local area network
(LAN) and how a network administrator can integrate this LAN into your com-
pany network. The description applies to the operating system Windows® and
all conventional routers.
Note: To set up a LAN, we recommend to use a router. That means the follow-
ing steps are required:
Process:
1. On the computer, go to the control panel and check the LAN properties.
2. Hook up the router to the devices and the computer.
3. On the computer, configure the router to set up the network.
4. Install the chromatography software from the data storage device.
5. Switch on the device and run the chromatography software.
Configuring the LAN Settings
The LAN uses only one server (which is normally the router) from that the
devices automatically receive their IP address.
Prerequisite:
In Windows®, power saving, hibernation, standby, and screen saver must be
deactived.
Process Figure
1. Place the terminal strip 3on a
suitable surface.
2. Push the depressor tool 1into
the opening on the upper side.
3. Continue pushing the depressor
tool down and lead the cable 2
into the front end of the termi-
nal strip.
4. Remove the depressor tool.
5. Check whether the cables are
tightly attached.
6. Plug the terminal strip onto the
multi-pin connector. Fig. 7 terminal strip
1
2
3

18 Unpacking and Setup
P 2.1S/P 4.1S user manual V6870, version 3.1
In case you use an USB-to-COM box, the option "Allow the computer to turn
off ths device to save power" in the devicemanager must be deactivated for
all USB hosts.
Only for Windows 7: For the network adapter, the option "Allow the com-
puter to turn off this device to save power" in the Device Manager must be
deactivated.
Process:
1. In Windows 7 choose Start
 Control Panel  Network and Sharing Center.
2. Double-click on LAN Connection.
3. Click on the button Properties.
4. Select Internet Protocol version 4 (TCP/IPv4).
5. Click on the button Properties.
6. Check the settings in the tab General. The correct settings for the DHCP cli-
ent are:
a) Obtain IP address automatically
b) Obtain DNS server address automatically
7. Click on the button OK.
Connecting the Cables
A router 3has several LAN ports 2and one WAN port 4that can be used to
integrate the LAN into a wide area network (WAN), e.g. a company network or
the Internet. In contrast, the LAN ports serve to set up a network from devices
1and a computer 5. To avoid interference, we recommend operating the
HPLC system separately from the company network.
You will find patch cables for each device and the router in the accessories kit.
To connect the router to a WAN, an additional patch cable is required, which is
not supplied within the scope of delivery.
Prerequisite:
The computer has been switched off.
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There is a patch cable for each device and the computer.
Process:
1. Use the patch cable to connect the router and the computer. Repeat this
step to connect all devices.
2. Use the power supply to connect the router to the mains power system.
Configuring the Router
The router is preset at the factory. You will find a label at the bottom side of the
router, on which IP address, user name, and password are printed. These infor-
mation help to open the router configuration.
Process:
1. To open the router configuration, start your Internet browser and enter the
IP address (not for all routers).
2. Enter user name and password.
3. Configure the router as DHCP server.
4. In the router configuration, check the IP address range and make changes if
necessary.
Result:
Once the router has assigned IP addresses to all devices, the chromatography
software can be used to remotely control the system.
Integrating the LAN into a Company Network
A network administrator can integrate the LAN into your company network. In
this case you use the WAN port of the router.
Prerequisite:
There is a patch cable for the connection.
Process:
1. Check that the IP address range of the router and of the company network
do not overlap.
2. In case of an overlap, change the IP address range of the router.
3. Use the patch cable to connect the router WAN port to the company net-
work.
4. Restart all devices, including the computer.
Controlling Several Systems Separately in a LAN
Devices connected to a LAN communicate through ports, which are part of the
IP address. If more than one HPLC system is connected to the same LAN and
you plan on controlling them separately, you can use different ports to avoid
interference. Therefore, the port number for each device must be changed and
this same number must be entered into the device configuration of the chroma-

20 Operation
P 2.1S/P 4.1S user manual V6870, version 3.1
tography software. We recommend to use the same port number for all devices
in the same system.
Note: The port is set to 10001 at the factory. You must use the same numbers
in the device configuration of the chromatography software as in the device,
otherwise the connection fails.
Process:
1. Find out port number and change it on the device.
2. Enter the port number in the chromatography software.
Result:
The connection is established.
Ground
Electronic defect
Electronic hazard when using an identically constructed power adapter from
another manufacturer.
Contact the Technical Support of KNAUER.
Never connect the device to the power connection.
The ground connection for the pump has a designated hole with a thread M3
on the back of the device.
If the supplied power adapter is used, then the ground connection remains
unused.
Please contact the technical support of KNAUER, if the pump along with
other devices should be connected to the power supply with a 6-prong
power adapter; a pump needs to be grounded exclusively.
Operation
Switching on the Pump
Prerequisite: In analog control mode, close the contact between GROUND and
START IN before starting the pump.
Component defect
Damage to the pump head in case it runs dry.
Ensure that liquid runs through pump head and piston backflushing.
Process:
1. Connect the power adapter to the power supply.
2. Connect the pump with plug from the external power adapter.