Knauer BlueShadow 40P User manual

Pump 40P
Instructions
Document no. V7613
HPLCHPLC

Note: For your own safety, read the instructions and observe the war-
nings and safety information on the device and in the instructions.
Keep the instructions for future reference.
Note: In case you require this instruction in another language, please
submit your request including the corresponding document number via
e-mail or fax to KNAUER.
Do you have questions about the installation or the operation of your
instrument or software?
International Support:
Contact your local KNAUER partner for support:
www.knauer.net/en/Support/Distributors-worldwide
Support in Germany
(Austria & Switzerland on case-to-case basis):
Phone: [phone] (workdays 9-17h CET)
Fax : [phone]
Email: [e-mail]
KNAUER Wissenschaftliche Geräte GmbH
Hegauer Weg 38
14163 Berlin
Germany
Phone: [phone]
Fax: [phone]
Internet: www.knauer.net
E-Mail: [e-mail]
Document number: V7613
Version number: 1.0
Release date: 2020/11/06
Translation of the original edition
The information in this document is subject to change
without prior notice. For the latest version of the instructions,
visit our website: www.knauer.net/library.
The printed versions of our instructions are printed according to Blue
Angel standards (www.blauer-engel.de/en/uz195).
This document contains confidential information and may not be
reproduced without written consent of KNAUER Wissenschaftliche
Geräte GmbH.
© KNAUER Wissenschaftliche Geräte GmbH 2020
All rights reserved.
Support:
Publisher:
Version information:
Sustainability:
Copyright:

BlueShadow Pump 40P Instructions V7613
Table of contents
1. General ..............................................................................1
1.1 About these instructions.........................................................1
1.2 Signal words ...................................................................1
1.3 Additional typographical conventions .............................................1
1.4 Legal information ...............................................................2
1.4.1 Liability limitation......................................................2
1.4.2 Transport damage .....................................................2
1.4.3 Warranty conditions ...................................................2
1.4.4 Warranty seal .........................................................2
1.4.5 Declaration of conformity...............................................2
2. Basic safety instructions ................................................................3
2.1 Intended use...................................................................3
2.1.1 Operating ranges .....................................................3
2.1.2 Foreseeable misuse....................................................3
2.2 User qualification ...............................................................3
2.3 Operator responsibility..........................................................4
2.4 Personal safety equipment .......................................................4
2.5 Working with solvents ...........................................................5
2.5.1 General requirements..................................................5
2.5.2 Contamination by health-threatening solvents.............................5
2.5.3 Avoiding leakage......................................................5
2.6 Specific environments...........................................................6
2.6.1 Earthqake-endangered areas ...........................................6
2.6.2 Explosive environment .................................................6
2.6.3 Cooling room .........................................................6
2.6.4 Wet room ............................................................6
2.7 Maintenance, care and repair ....................................................6
2.8 Service request form and decontamination report ..................................7
3. Product information ...................................................................8
3.1 Views .........................................................................8
3.1.1 Front view ............................................................8
3.1.2 Rear view .............................................................8
3.2 Features .......................................................................9
3.3 Pump heads ...................................................................9
3.4 RFID recognition ...............................................................9
3.5 Symbols and signs .............................................................10

BlueShadow Pump 40P Instructions V7613
3.6 Scope of delivery ..............................................................10
4. Installation and initial startup ..........................................................11
4.1 Unpacking....................................................................11
4.2 Ambient conditions ............................................................11
4.2.1 Operation site........................................................11
4.2.2 Ambient temperature .................................................11
4.2.3 Space requirements ..................................................12
4.3 Power supply .................................................................12
4.4 Electric connections ...........................................................13
4.4.1 Connecting cables to the pin header....................................13
4.4.2 Remote connector ....................................................14
4.4.3 Events connector .....................................................15
4.5 Connecting capillaries and fittings ...............................................15
4.5.1 Connecting the solvent bottles .........................................16
4.5.2 Connecting the pump head to the solvent ...............................16
4.6 Control.......................................................................18
4.6.1 Setting up LAN.......................................................18
4.6.2 Configuring LAN settings..............................................19
4.6.3 Connecting devices with LAN ..........................................19
4.6.4 Configuring the router ................................................20
4.6.5 Integrating LAN into the company network ..............................21
4.6.6 Controlling several systems separately in LAN............................21
4.7 Setting IP addresses via software ................................................21
4.7.1 Firmware Wizard: Setting a static IP address..............................22
4.7.2 Firmware Wizard: Setting a dynamic IP address...........................22
5. Operation ...........................................................................24
5.1 Initial operation ...............................................................24
5.1.1 Pump head running-in ................................................24
5.1.2 Isocratic operating mode ..............................................25
5.1.3 Optimizing HPLC separations ......................................25
5.2 Purging the pump .............................................................25
5.2.1 Piston backflushing ...................................................26
5.3 Switch on and self test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
5.4 Operating with LC display and keypad ...........................................27
5.4.1 LED status ...........................................................28
5.4.2 RFID icon............................................................28
5.4.3 Operating with the keypad ............................................29
5.5 Menu structure ................................................................30
5.5.1 Main menu ..........................................................30
5.5.2 Menu structure: overview..............................................31
5.5.3 Device setting parameters .............................................32

BlueShadow Pump 40P Instructions V7613
5.6 GLP menu ....................................................................35
5.7 Program menu ................................................................35
5.7.1 Create program ......................................................36
5.7.2 Run program.........................................................36
5.7.3 Change program .....................................................37
5.7.4 Delete program lines .................................................37
5.7.5 Delete a program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
5.7.6 Create a program with a starting time ...................................37
5.8 Link menu ....................................................................38
5.8.1 Create link...........................................................38
5.8.2 Run link .............................................................39
5.8.3 Delete link...........................................................39
6. Functionality tests ....................................................................40
6.1 Installation Qualification (IQ) ....................................................40
6.2 Operation Qualification (OQ) ...................................................40
7. Troubleshooting......................................................................41
7.1 LAN..........................................................................41
7.2 Possible problems and solutions.................................................42
7.3 System messages..............................................................43
8. Maintenance and care ................................................................46
8.1 Maintenance contract ..........................................................46
8.2 Cleaning and caring for the device...............................................46
8.3 Checking the fittings ...........................................................46
8.4 Flushing the pump.............................................................47
8.5 Maintaining the pump head.....................................................48
8.5.1 Dismounting the pump head ..........................................48
8.5.2 Check valves.........................................................50
8.6 Maintaining the filter cartridge at
pressure sensor .....................................................................52
8.6.1 Removing filter cartridge ..............................................52
8.6.2 Inserting a new filter cartridge..........................................53
9. Transport and storage.................................................................55
9.1 Putting the pump out of operation ...............................................55
9.2 Packing the device.............................................................55
9.3 Transporting the device ........................................................55
9.4 Storing .......................................................................55
9.4.1 Storing the device ....................................................55
9.4.2 Storing the pump head................................................56

BlueShadow Pump 40P Instructions V7613
10. Disposal.............................................................................57
10.1 AVV-Marking Germany .........................................................57
10.2 WEEE registration number ......................................................57
10.3 Eluents and other operating materials ............................................57
11. Technical data........................................................................58
11.1 Solvent delivery ...............................................................58
11.2 Communication ...............................................................59
11.3 Technical parameters ..........................................................59
11.4 General ......................................................................59
12. Chemical compatibility of wetted materials..............................................60
12.1 General ......................................................................60
12.2 Plastics .......................................................................60
12.3 Non-metals ...................................................................62
12.4 Metals. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63
13. Repeat orders........................................................................64
13.1 Devices ......................................................................64
13.2 Spare parts ...................................................................64

1
BlueShadow Pump 40P Instructions V7613
General
1. General
1.1 About these instructions
These operating instructions enable the safe and efficient operation of
the device. The user must have carefully read and understood these ope-
rating instructions before starting any work.
The basic prerequisite for safe operation is compliance with all safety
instructions (see chapter 2 on page 3). In addition to the safety and
warning instructions in these operating instructions, the local accident
prevention regulations and the national industrial safety regulations
apply.
These operating instructions are an integral part of the device. It must be
kept in the immediate vicinity of the device and accessible to the user at
all times.
You can download these and other instructions from the KNAUER web-
site: www.knauer.net/library
1.2 Signal words
Possible dangers related to the device are distinguished in personal and
material damages.
Symbol Meaning
DANGER (red) indicates a highly hazardous situa-
tion. If not avoided, it will result in death or serious
injury.
WARNING (orange) indicates a hazardous situa-
tion. If not avoided, it could result in death or
serious injury.
CAUTION (yellow) indicates a moderate hazardous
situation. If not avoided, it could result in minor or
moderate injury.
NOTICE (blue) is used to address issues which are
not related to physical injury.
1.3 Additional typographical conventions
General equal treatment: When persons are described, this document
uses the male grammar form to keep the text easy to read. The form
has a neutral sense and speaks to people of any gender in the same
way.
Note: Specific information are prefixed with the word "Note” and an
information icon.
Note: This is an example.

2General
BlueShadow Pump 40P Instructions V7613
1.4 Legal information
1.4.1 Liability limitation
The manufacturer is not liable for the following issues:
Non-compliance of these instructions
Non-observance of necessary safety precautions
Improper use
Operation of the device by unqualified personnel (see chapter 2.2 on
page 3)
Use of non-approved spare parts
Technical changes by the user such as opening the device and unaut-
horized modifications
Violations of General Terms and Conditions (GTC)
1.4.2 Transport damage
The packaging of our devices provides the best possible protection
against transport damage. However, check the packaging for transport
damage. In case you notice any damage, inform the Technical Support
and the shipping company within three workdays.
1.4.3 Warranty conditions
For information on warranty please refer to our general terms and condi-
tions on the website: www.knauer.net/terms
1.4.4 Warranty seal
A blue or orange warranty seal is affixed to some devices.
A blue seal is used by KNAUER’s Manufacturing or Customer Support
for devices to be sold.
After repair, service technicians attach an orange seal onto the identi-
cal position.
After repair, the service technician affixes an orange seal in the same pla-
ce. If unauthorised persons tamper with the device or if the seal is dama-
ged, the warranty will lapse.
1.4.5 Declaration of conformity
The declaration of conformity is enclosed as a separate document with
the product and can be obtained online:
www.knauer.net/en/Support/Declarations-of-conformity

3
BlueShadow Pump 40P Instructions V7613
Basic safety instructions
2. Basic safety instructions
The device has been developed and constructed in such a way that ha-
zards arising from its intended use are largely excluded. Nevertheless, the
following safety instructions must be observed in order to exclude residu-
al hazards.
2.1 Intended use
Only use the device for applications that fall within the range of the inten-
ded use. Otherwise, the protective and safety equipment of the device
could fail.
2.1.1 Operating ranges
The device is intended to be used indoors for chromatographic
applications.
2.1.2 Foreseeable misuse
Refrain from the use of the device for the following purposes or
conditions:
Medical purposes. The device is not approved as a medical product.
Operating outdoors. Otherwise, the manufacturer does not guarantee
the functionality and safety of the device.
Operation in potentially explosive areas without special and additio-
nal explosion protection. Contact the KNAUER Customer Support for
more information.
2.2 User qualification
The user is qualified to handle the device if all of the following points
apply:
He has at least a basic knowledge of liquid chromatography.
He has knowledge about the properties of the used solvents and their
health risks.
He is trained for the special tasks and activities in the laboratory and
knows the relevant standards and regulations.
Due to his technical training and experience, he can understand and
carry out all the work described in the operating instructions on the
instrument and recognize and avoid possible dangers independently.
His ability to react is not impaired by the consumption of drugs,
alcohol or medication.
Participation in the installation of a device or a training by the company
KNAUER or an authorized company.
If the user does not meet these qualifications, he must inform his
supervisor.

4Basic safety instructions
BlueShadow Pump 40P Instructions V7613
2.3 Operator responsibility
The operator is any person who operates the device himself or leaves it to
a third party for use and who bears the legal product responsibility for the
protection of the user or third parties during operation.
The obligations of the operator are listed below:
Know and follow the applicable work safety regulations
Identify hazards arising from the working conditions at the place of use
in a risk assessment.
Set up operating instructions for the operation of the device.
Regularly check whether the operating instructions correspond to the
current status of the regulations.
Clearly regulate and specify responsibilities for installation, operation,
troubleshooting, maintenance and cleaning and set clear rules
Ensure that all personnel who work with the device have read and un-
derstood these operating instructions
Train the personnel who work with the device at regular intervals and
inform them about the dangers.
Provide the necessary safety equipment to the employees working
with the unit (see section below).
2.4 Personal safety equipment
The protective measures required in the laboratory must be observed
and the following protective clothing worn during all work on the device:
Safety glasses with side protection
Protective gloves in accordance with the prevailing ambient conditions
and used solvents (e.g. heat, cold, protection against chemicals)
Lab coat
Personalised protective safety equipment which is specified in the
particular laboratory.

5Basic safety instructions
BlueShadow Pump 40P Instructions V7613
2.5 Working with solvents
2.5.1 General requirements
The user is trained for handling different solvents.
Note recommended solvents and concentrations in these instructions
in order to avoid personal injury or damage to the device. For exam-
ple, certain chemicals may cause PEEK capillaries to swell or burst (see
chapter 12 on page 60).
Note that organic solvents are toxic above a certain concentration. For
handling hazardous solvents see the following section.
Mobile phases and samples may contain volatile or combustible sol-
vents. Avoid the accumulation of these substances. Ensure good ven-
tilation of the installation site. Avoid open flames and sparks. Do not
operate the instrument in the presence of flammable gases or vapors.
Only use solvents which do not self-ignite under given conditions. This
applies especially to the use of a thermostat where liquids could get
onto hot surfaces in the interior.
Degas solvents before use and observe their purity.
2.5.2 Contamination by health-threatening solvents
Contamination with toxic, infectious or radioactive substances poses
a hazard for all persons involved during operation, repair, sale, and dis-
posal of a device.
All contaminated devices must be properly decontaminated by a spe-
cialist company or the operating company before they can be recom-
missioned, repaired, sold, or disposed (see chapter 10 on page 57).
2.5.3 Avoiding leakage
Risk of electrical shock or short circuit if solvents or other liquids leak into
the interior of the device. You can avoid a leakage through the following
measures:
Tightness: Visually check the device or system regularly for leaks.
Solvent tray: The use of a solvent tray prevents liquids get from the
bottles into the inside of the device.
Eluent lines: Install capillaries and hoses in such a way that, in case of a
leak, liquids cannot get into the interior of the devices underneath.
In case of leakage: Switch off the system. Only take the device into
operation if the cause of the leak has been resolved (see chapter 8 on
page 46).

6Basic safety instructions
BlueShadow Pump 40P Instructions V7613
2.6 Specific environments
2.6.1 Earthquake-endangered areas
In earthquake-endangered areas, do not stack more than 3 devices on
top of each other. Otherwise there is a risk of injury due to falling devices
or loose parts.
2.6.2 Explosive environment
Never use the system in potentially explosive atmospheres without
appropriate protective equipment. For more information, contact the
KNAUER Customer Support.
2.6.3 Cooling room
You may operate the device in a cooling room. To prevent condensation,
note the following instructions:
Allow the device to acclimatize for min. 3 hours before taking it into
operation.
After taking into operation, the device should stay switched on.
Avoid temperature fluctuations.
2.6.4 Wet room
The device must not be operated in wet rooms.
2.7 Maintenance, care and repair
Avoiding electric shock: Before performing any maintenance and ser-
vice work, disconnect the device from the power supply.
Tools: Use only tools recommended or prescribed by the
manufacturer.
Spare parts and accessories: Only use original parts and accessories
made by KNAUER or a company authorized by KNAUER.
PEEK fittings: Use PEEK fittings only for a single port or brand-new
PEEK fittings in order to avoid dead volume or not exactly fitting
connections.
Column care: Follow KNAUER or other manufacturer's instructions on
caring for the columns (see www.knauer.net/columncare)
Used capillaries: Do not use any used capillaries elsewhere in the
system in order to avoid dead volumes, not exactly fitting connections
and spreading contamination.
Safety features: The device may only be opened by the KNAUER Cus-
tomer Support of KNAUER or any company authorized by KNAUER
(see chapter 1.4.1 on page 2).
For more information visit the KNAUER website:
www.knauer.net/hplc-troubleshooting

7Basic safety instructions
BlueShadow Pump 40P Instructions V7613
2.8 Service request form and decontamination
report
Devices which are shipped without the completed document “Service
request form and decontamination report” will not be repaired. If you
would like to return a device to KNAUER, make sure to enclose the com-
pleted document: www.knauer.net/servicerequest

8Product information
BlueShadow Pump 40P Instructions V7613
3. Product information
3.1 Views
3.1.1 Front view
Legend
1 Display
2 Pressure sensor
3 Ventilation screw
4 Keypad
5 Tube connections for
the piston backflushing
6 Pump head
Fig. 1: Front view
3.1.2 Rear view
Legend
1 Warranty seal
2 Space for rear label
(with serial number)
3 Fan opening
4 Warning 1
5 Power switch
6 Power supply
7 CE-Mark
8 Remote connector
9 RS-232 Port
blWarning 2
bmLAN Connection
bnEvents connector
Fig. 2: Rear view
5
12
36
bl
4
123 4
bn bm 9 8 7
5
6

9Product information
BlueShadow Pump 40P Instructions V7613
3.2 Features
Dual-piston technology combined with optimized electronic pulsation
dampening and compressibility compensation
Liquid transport with low pulsation, stable flow rate and high flow
accuracy
Pump head made of stainless steel or with ceramic inlays
Automatic piston backflushing
High physical and chemical stability
Direct pump control via analog and digital signals
Control with chromatography software
3.3 Pump heads
Note: Due to the new drive used in the pump, pump heads of older se-
ries cannot be used any more.
Available pump head types:
Pump head 10ml, for use in analytical applications, standard version
made of stainless steel. Ceramic inlays forbiocompatible applications.
Pump head 50ml, for use in semi-preparative applications, standard
version made of stainless steel. Ceramic inlays for biocompatible
applications.
The front of the pump head is labeled with the max. pumping capacity
(10ml or 50ml). Pump heads with inlays carry additional material labels
(SST for stainless steel, C for ceramic).
The pump automatically recognizes the pump head by means of an RFID
chip.
3.4 RFID recognition
The pump head is equipped with an RFID chip. It is used to monitor and
save all important parameters and settings of thepump and pump head.
The RFID technology offers the following advantages:
Automatic detection of all important pump head parameters by means
of radio signals (radio frequency identification), bythe pump software
or the chromatography software:
- Pump head type
- Serial number and year of manufacture
- Number of cycles and operating times
- Limit values of the pump head parameters
All measuring data archived in accordance with GLP (goodlaboratory
practice)
Transfer of all data to the software and pump display
Labeling on pump heads
Automatic pump head
recognition

10 Product information
BlueShadow Pump 40P Instructions V7613
3.5 Symbols and signs
The following symbols and signs can be found on the device:
Symbol Meaning
High-voltage hazard
Electrostatic discharge hazard, damages to
system, device, or components can occur.
A device marked with CE fulfills the product spe-
cific requirements of European directives. This is
confirmed in a Declaration of Conformity.
A warranty seal is affixed to some devices. For
more information see chap. 1.4.4 on p. 2.
3.6 Scope of delivery
Note: Only use original parts and accessories made by KNAUER or a
company authorized by KNAUER.
Power cable
BlueShadow Pump 40P with pump head
Accessories kit pump 40P
Accessories kit pump head
Applicable documents:
User manual
Installation Qualification
Declaration of Conformity

11Installation and initial startup
BlueShadow Pump 40P Instructions V7613
4. Installation and initial startup
Before you determine the operation site, read the chapter “Technical
data” (see chapter 11 on page 58). There you will find all device-speci-
fic information on power supply, ambient conditions and humidity.
Note: The intended use be ensured only if the requirements for ambient
conditions of the operating environment are met.
4.1 Unpacking
Process
1. Place the packaging in such a way that the lettering on the label is
in the correct position.
2. Check the packaging, the device and the accessories for transport
damage.
3. Check the scope of delivery. In the event of incomplete delivery,
contact technical support immediately.
4. When lifting, carrying or moving the device, grab the unit only
from below on the sides. Do not hold onto front cover or leak tray,
as these parts are loosely attached to the device.
Keep the included packing list for repeat orders.
Keep the original packaging for safe storage or transportation.
4.2 Ambient conditions
4.2.1 Operation site
Observe the following requirements for the operation site so that the
measurement results are not influenced:
Place on a firm, level and straight surface.
Protect against direct sunlight.
Do not expose to air drafts such as air conditioning systems.
Do not set up the to other machines that cause floor vibrations.
Keep from high frequency sources.
Ensure adequate ventilation (see chapter 4.2.3 on page 12).
Avoid temperature fluctuations (see chapter 4.2.2 on page 11).
4.2.2 Ambient temperature
If the ambient temperature of the device is abruptly changed (e.g. when
it is installed in a cooling chamber), condensation will form inside the
device and may cause damage to the device. Allow the device to accli-
mate for 3 h, before it is connected to the power supply and taken into
operation.
Procedure
Next steps

12 Installation and initial startup
BlueShadow Pump 40P Instructions V7613
4.2.3 Space requirements
Make sure that the power plug on the power supply (wall socket or
power strip) is always accessible, so that the device can be disconnec-
ted from the power supply.
Ensure adequate ventilation around the device, otherwise it may over-
heat and malfunction:
- Min. 5 cm distance if another device is set on one side.
- Min. 10 cm distance if further devices are set on both sides.
- At least 15 cm to the cooler fan on the rear.
4.3 Power supply
Power supply requirements
Failure-free power supply: For failure-free operation, the electrical
voltage must be free of fluctuations, residual currents, voltage peaks
and electromagnetic interference. The device must receive sufficient
voltage and reserve capacity.
Check voltage: Only connect devices to a power supply whose voltage
corresponds to the permissible voltage of the device.
Power consumption: The nominal power of the connected devices
must not exceed 50 % of the highest connected power capacity, since
higher currents can flow briefly when the device is switched on.
Main connection: The electrical power supply at the operation site
must be connected directly to the nearest main power connection.
Grounding: The connectors for the voltage must be grounded
accordingly.
Power supply cables and plugs
Original parts: For power supply, use the supplied power cable and
plug to meet the specifications which are described in the chapter
“Technical data” (see chapter 11 on page 58). Detachable power
cables are not allowed to be replaced with other cable types.
Country-specific plugs: Before switching on the device, check whether
the supplied plug is approved for your country. Overview of the de-
vice- and country-specific plug types from KNAUER:
www.knauer.net/plugs.
Power strips: If several devices are connected to one power strip, al-
ways consider the maximum power consumption of each device.
Access to power supply: Make sure that the power plug on the pow-
er supply (wall socket or power strip) is always accessible, so that the
device can be disconnected easily from the power supply.
Damaged power supply cables and plugs: For safety reasons, dama-
ged or faulty cables and plugs must not be used to connect the device
to the power supply. Replace defective cables and plugs only with
KNAUER accessories.

13Installation and initial startup
BlueShadow Pump 40P Instructions V7613
4.4 Electric connections
Use the Events and Remote pin header to connect the pump with ex-
ternal devices.
Use the LAN connection to connect the pump with external devices
within a network.
Alternatively, connect the pump to a computer withthe RS-232 port.
4.4.1 Connecting cables to the pin header
To control one device through another, the pin header is used. To use
remote control, you have to connect cables to the pin header. The single
ports are used to exchange control signals.
The device is switched off.
The power plug is pulled.
Depressor tool
Electronic defect
Connecting cables to the multi-pin connector of a switched on device
causes a short circuit.
Turn off the device before connecting cables.
Pull the power plug.
2
3
1
Fig. 3: Depressor tool
Tool
Electronic defect
Electrostatic discharge can destroy the electronics.
Wear a protective bracelet against electrostatic discharge and ground.

14 Installation and initial startup
BlueShadow Pump 40P Instructions V7613
Process
1. Push the depressor tool
3into an upper small opening on the
front of the terminal strip 1.
2. Lead the cable into the opening 2below the inserted operating
tool.
3. Remove the depressor tool.
Check if the cables are firmly connected. Push the terminal strip onto the
pin header. Finish the installation. Put the device into operation.
4.4.2 Remote connector
For receiving start, control and error signals from external devices
For sending start, control and error signals to external devices
Fig. 4: Remote connector
Next steps