Knauer K-1900 User manual

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V7062 02/2017
KNAUER
Wissenschaftliche Geräte GmbH
Hegauer Weg 38
D - 14163 Berlin, Germany
Tel.: [phone]
Fax: [phone]
E-Mail: [e-mail]
Internet: www.knauer.net

3
General Description
Contents
General Description.......................................................................................................................................3
Using this Manual..........................................................................................................................................4
Conventions in this Manual...................................................................................................................4
SOP’s in this manual.............................................................................................................................4
Technical Data of Pneumatic Pump K–1900.................................................................................................5
Setup of the Pneumatic Pump K–1900 .........................................................................................................5
Unpacking..............................................................................................................................................5
Front View of the Pneumatic Pump K–1900..........................................................................................6
Connecting the Pneumatic Pump K–1900.....................................................................................................7
Compressed Air Connections................................................................................................................7
Pump Heads and Hydraulic Connections..............................................................................................7
Identifying installed Pump Heads...................................................................................................7
Pump Heads and Hydraulic Connections..............................................................................................8
100, 250 and 500 ml Head ............................................................................................................8
1000 ml Head.................................................................................................................................8
Setting up the K-1900............................................................................................................................9
Piston Backflushing........................................................................................................................9
Air Pressure Influences........................................................................................................................10
Calibration for Constant Flow ..............................................................................................................11
Easy Maintenance.......................................................................................................................................12
Packing List.................................................................................................................................................16
Spare Parts and Accessories......................................................................................................................17
Declaration of conformity.............................................................................................................................34
Inhalt
Allgemeine Beschreibung der Pneumatischen Pumpe K–1900...................................................................18
Hinweise zum Gebrauch des Handbuchs...................................................................................................19
Konventionen in diesem Handbuch ....................................................................................................19
SOP's in diesem Handbuch ................................................................................................................19
Technische Daten der Pneumatischen Pumpe K–1900.............................................................................20
Inbetriebnahme der Pneumatischen Pumpe K-1900..................................................................................20
Auspacken...........................................................................................................................................20
Frontansicht der Pneumatischen Pumpe K-1900 ...............................................................................21
Anschluss der Pneumatischen Pumpe K–1900..................................................................................22
Pumpenköpfe und Lösungsmittelanschlüsse......................................................................................22
Pumpenkopfidentifikation ............................................................................................................22
Flüssigkeitsverbindungen............................................................................................................23
100, 250 und 500 ml Kopf ...........................................................................................................23
1000 ml Kopf................................................................................................................................23
Betriebsvorbereitung...........................................................................................................................24
Pressluftdruck und Flüssigkeitsdruck und -fluss.................................................................................25
Kalibrierung für konstanten Fluss........................................................................................................26
Wartung durch den Anwender ....................................................................................................................27
Austausch und Reinigung des Pumpenkopfes...................................................................................27
Lieferumfang ...............................................................................................................................................31
Ersatzteile und Zubehör..............................................................................................................................32
Warranty statement.....................................................................................................................................33
Gewährleistungsbedingungen.....................................................................................................................33
Konformitätserklärung .................................................................................................................................34

3
General Description
General Description
The Pneumatic Pump K–1900 has been developed for solvent delivery within
the following fields:
• Process chemistry
• Column packing
• Preparative chromatography.
It is powered by pressurized air. It can deliver liquids with an adjustable flow
rate. Since no electrical connection is required, the Pneumatic Pump K–1900 is
suitable for operation in explosion protected environments.
The new Pneumatic Pump K-1900 has been developed for constant pressure
applications. It is suitable for packing high performance columns (microbore,
analytical and preparative) up to 1000 bar. The flow is highly reproducible and
only little maintenance efforts are required.
It features a double-piston design and can be configured with four different
pump heads to meet a broad range of requirements in flow and pressure
ranges:
100 ml pump head (0.1 – 99.9 ml/min), stainless steel, up to 1000 bar
250 ml pump head (0.1 – 249.9 ml/min), stainless steel, up to 200 bar
500 ml pump head (0.1 – 499.9 ml/min), stainless steel, up to 100 bar
1000 ml pump head (0.1 – 999.9 ml/min), stainless steel, up to 50 bar
The names of the pump heads have historical reasons. You can also operate
any of the mentioned pump heads with our Preparative HPLC Pump K-1800. In
the case of the Pneumatic Pump K–1900 the piston strokes operate with a
higher frequency and therefore flow rates can be much higher than specified on
the pump head.
The characterisics of flow and liquid pressure for the K-1900 with the different
pump heads are listed in Table 2, „Technical data of individual pump heads“ on
page 6.
Pump heads can be interchanged by loosening only four screws. Experienced
users can disassemble the heads for simple maintenance tasks like cleaning,
changing piston seals, etc.
A cooling device (heat exchanger) to be mounted on the pump head is
available. It allows to operate the Pneumatic Pump K–1900 with supercritical
solvents as CO2 for Extraction and Supercritical Fluid Chromatography.
The power unit of the pump consists of a pneumatic piston which is set in
motion by compressed air. The pneumatic power will be trans-mitted to the
piston rods by the power transmit lever.

Using this Manual
4
Using this Manual
Conventions in this Manual
Special Warnings are indicated by the marginal warning sign and printed in
bold letters.
The marginal lamp symbol indicates helpful advice’s.
Important Hints are marked by the marginal hand symbol.
SOP’s in this manual
The Standard Operating Procedures (SOP) provided with this manual offer a
convenient way of structuring complex tasks in the operation of your WellChrom
Pneumatic Pump K–1900. They include step-by-step instructions leading the
user through all routine tasks during operation. They can be used for
documentation purposes and be copied, applied signed, and filed in order to
document the performance of the instrument.
Please operate the instrument and all accessories according to
instructions and SOP’s in this manual. This ensures proper results and
longevity of your equipment.
Table 1 SOP’s in this manual
SOP
1
Preparing your K–1900 for piston backflushing
10
SOP
2
Start of Solvent Delivery
11
SOP
3
Removing the Pump Head
13
SOP
4
Removing and Checking Piston Rods
13
SOP
5
Disassembling the Pump Head
14
SOP
6
Assembling the Pump Head and Exchanging Piston Seals
15
SOP
7
Installing the Pump Head
15
SOP
8
Cleaning and Replacing Check Valves
16

5
Technical Data of Pneumatic Pump K–1900
Technical Data of Pneumatic Pump K–1900
Table 2 Technical data of individual pump heads
Characteristics Pump Head
100 ml 250 ml
500ml
1000 ml
Maximum Flow Rate (ml/min) at
300
500
1000
2000
Minimum Back Pressure
Maximum Back Pressure (bar) for
1000
200
100
50
Solvent Delivery
Maximum Allowed Air Pressure (bar) 4.2
*)
7 7 7
Piston Stroke (mm)
9.5
9.5
9,5
9.5
Stroke Volume per Cycle (ml)
0.6
1.35
3,05
5.4
Diameter (mm)
6.35
9.525
14,29
19.05
Pneumatic Transformation Ratio
1:250
1:96
1:47
1:23
*) See warning below.
With the 100 ml pump head the external air pressure must under no
circumstances ever exceed 4.2 bar. Exceeding an external air pressure
of 4.2 bar will lead to damage of pump head and pump mechanics. This
damage is not covered by guarantee.
Setup of the Pneumatic Pump K–1900
Unpacking
Unpack the instrument and check pump and accessories for any damage due to
shipping. Place any claims referring to damage at the transportation company
responsible for shipping.
Please use the packing list and make sure that the delivery of the Pneumatic
Pump K-1900 is complete. If you have any questions regarding complete
delivery, please call our service department as soon as possible.
Please remove the protection foil from pump outlet.
For the complete list of spare parts available please refer to chapter „Spare
Parts and Accessories“ on page 18.

Setup of the Pneumatic Pump K–1900
6
Front View of the Pneumatic Pump K–1900
All operating elements and hydraulic connections are located on the front of the
pump, see Fig. 1 and Fig. 2.
3 4
2
5
1
1 Pump head
2 Flushing Valve
3 Eluent pressure gauge
4 Air pressure gauge
5 Regulator for compressed air
Fig. 1 Front View of the Pneumatic Pump K–1900
1.8
2.1
2.2
2.3
2.4
1.7
1.1
1.6
1.3
1.4
1.5
1.2
1 Pump Head 2 Flushing Valve
1.1 Piston backflushing capillaries 2.1 Inlets to flushing valve
1.2 Eluent inlets 2.2 De-aeration capillary 1/16“
1.3 Head set screws 2.3 De-aeration screw
1.4 Label with type of pump head 2.4 Eluent outlet to column
1.5 Inlets valve housing
1.6 Outlets valve housing
1.7 Eluent outlets to flushing valve
1.8 Connection capillaries
Fig. 2 Pump head and flushing valve

7
Connecting the Pneumatic Pump K–1900
Connecting the Pneumatic Pump K–1900
Compressed Air Connections
Make sure that your compressed air system to be used with the Pneu-matic
Pump K–1900 is able to maintain pressures up to 7 bar. With-out back pressure
the air consumption would be 80 L/min. If the Pneumatic Pump K-1900 works
against pressure the consumption of air is much lower depending on the liquid
pressure.
The connector to the compressed air is located on the lower rear panel of the
pump. Connect the coupler for the pressure tube to the coupler socket. Push
the pressure tube (not delivered) from the com-pressed air supply over the
coupler and secure it with an appropriate clamp. Connect the coupler socket to
the compressed air connector of the pump (see Fig. 2).
To disconnect from the compressed air supply: Hold the brass part of the
coupler with one hand and pull the blue part of the coupler socket away from
the pump with the other hand.
Compressed
air supply
Pump
Coupler Coupler
socket Connector for
compressedair
Fig. 3 Air pressure connection of the K-1900
Pump Heads and Hydraulic Connections
The pump should be rinsed with a methanol-water mixture (1:9 v/v) before
first usage. Make sure that all hydraulic connec-tions are suitable for the
system pressure and flow rate of your liquid transportation system.
Identifying installed Pump Heads
Maximum flow rate, 100, 250, 500, or 1000 ml/min, is indicated on a label on
the front of the pump head. This values are valid for the HPLC-Pump K-1800.
Working with the pneumatic pump K-1900 the maximum flow rates are
approximately two times higher .
Fig. 4 Pump head label (1000ml head)

Connecting the Pneumatic Pump K–1900
8
Pump Heads and Hydraulic Connections
All operating elements and hydraulic connections are located on the front of the
pump, see Fig. 1 and Fig. 2.
Make sure that all hydraulic connections are suited for the system
pressure and flow rate of your HPLC System. During initial set-up
procedures, and also later when changing solvent, pay strict attention to
the miscibility of solvents to be used one after another, otherwise the
function of the check valves may be adversely affected (see Fig. 14 on page
16). By substitutions non-mis-cible solvents such as methanol and hexane,
rinse after using methanol with an intermediate solvent that is miscible
with both solvents in all proportion, e.g. isopropanol.
100, 250 and 500 ml Head
Solvent tubings are connected to the pump head according to Fig. 5. Make sure
that the flat side of the ferrule is directed to the pump head and that the fitting
screw is fastened hand tight. According to the local conditions the tubings can
be connected either to the inlets valve housing (A) or to the eluent inlet (B).
1 inlets valve housing
2 eluent inlet
3 ferrule
4 fitting screw
A B
Fig. 5 100, 250 and 500 ml pump head: hydraulic connections
1000 ml Head
Push the tubes directly over the connectors, and secure them by means of
appropriate clamps.
Fig. 6 1000 ml pump head: hydraulic connections

9
Connecting the Pneumatic Pump K–1900
Setting up the K-1900
Never run the Pneumatic Pump K–1900 without liquid in the pump head or
in the piston backflushing compartment. For piston backflushing, follow
the instructions below. Operation with-out liquid may lead to damage of
the pump seals in short time.
Piston Backflushing
Backflushing the piston removes traces of salt and other decontaminants from
the backside of the pistons.
Especially if you use saline solvents or buffer solution we highly recommend
that you continuously backflush in order to prevent crystallization which can
damage your piston seals.
For best protection and error free long time operation of the pump head it is
recommended always to backflush the piston with an appropriate solvent
(mostly water) according to the following SOP.
SOP 1 Preparing your K–1900 for piston backflushing
This SOP applies to the Pneumatic Pump K–1900.
1. Push a 1/16" ID tube over both piston backflushing capillaries.
Fig. 7 Piston backflushing capillaries
2. Place one low end of the tubing in a flask.
3. Fill the priming syringe with the backflushing liquid.
4. Connect the syringe with the other tubing.
5. Press liquid through the pump head, until it flows without any air bubbles
into the container.
6. Remove tubings from capillary opening.
If you want operation with continuous backflushing you can attach two
containers of rinsing liquid instead of the priming syringe. The containers should
be positioned that one container is located higher than the other, thus ensuring
liquid flow through the pump head without any assistance.
When not backflushing continuously, we recommend to connect both capillary
openings with a tubing to prevent vaporization of solvents and drying out of the
piston chamber.

Connecting the Pneumatic Pump K–1900
10
Liquid Pressure [bar]
SOP 2 Start of Solvent Delivery
This SOP applies to the Pneumatic Pump K–1900.
1. Screw a blind fitting to the ”Eluent outlet to column”, Pos. {2.4} in Fig. 2
and open the “De-aeration screw”, Pos. {2.3} for opening flushing valve.
2. Connect the Luer-Lock syringe with the “De-aeration capillary”, Pos. {2.2}
in Fig. 2 with the help of a short PTFE tube (ID 1,5 mm/ OD 2,1 mm). The
pump head needs to be filled with liquid by pulling eluent into the Luer-
Lock syringe until liquid enters the syringe.
3. Close the “De-aeration screw” and exchange the blind fitting with a
regular 1/8” fittings to connect this outlet with an injection system, a
column packing device, a chemical reactor or something else.
4. Pull the Regulator for compressed air, see Fig. 2 for loosening up. The
pneumatic can be set in operation by turning the Regulator for
compressed air to the right (required air pressure increases). The eluent
will be transported to flushing valve.
5. The pump should be stopped by turning the Regulator for compressed air
to the left (required air pressure decreases towards 0 bar) and any unit
(e.g. the column filling device) is connected to the solvent outlet.
Air Pressure Influences
The resulting eluent pressure increases or decreases as a function of
compressed air inlet pressure. Eluent pressure will nearly be constant, if the
compressed air inlet pressure remains constant. The Regulator for compressed
air can be locked by pressing it to wards the instrument.
The delivery volume depends on the required air pressure and the loss of
pressure along the column or any device that generates back pressure. You
can reach the maximum delivery volume at minimum eluent pressure below 1
bar and the maximum eluent pressure at the minimum flow rate. Fig. 8 up to
Fig. 10 show the delivery volume as a function of the eluent pressure for the
100, 250 and 1000 ml pump head, respectively. In those figures you can see
the limit values for the liquid pressure and the flow rate corresponding to Table
2, “Technical data of individual pump heads,” on page 6 and the maximum
allowed air pressure for each pump head. By the constant required air pressure
the flow rate increases if the liquid pressure decreases. The Figures should help
to choose the pump head for your application.
1200
800
400
0
Required Compressed Air
4,2 bar
4 bar
100 ml
3 bar
2 bar
1 bar
0 100 200 300
Flow Rate [ml/min]
Fig. 8 Interdependence of delivery volume and eluent pressure for the 100 ml pump
head

11
Connecting the Pneumatic Pump K–1900
RequiredCompressed Air
250 ml
7 bar
6 bar
5 bar
4 bar
3 bar
2 bar
1 bar
Requiredcompressed air
1000 ml
7 bar
6 bar
5 bar
4 bar
3 bar
2 bar
1 bar
-1
Flow Rate [ ml min ]
Liquid
Pressure
[bar]
Liquid Pressure [bar]
900
600
300
0 0 300 600 900
Flow Rate [ml/min]
Fig. 9 Interdependence of delivery volume and eluent pressure for the 250 ml pump
head
210
140
70
0
0 800 1600 2400
Flow Rate [ml/min]
Fig. 10 Interdependence of delivery volume and eluent pressure for the 1000 ml pump
head
Calibration for Constant Flow
If you want to use the pneumatic pump as a delivery pump with constant
delivery volume, you need to calibrate it.
Fig. 11 shows typical calibration curves for two different columns with different
pressure losses along the column for the 250 ml pump head. In this figure
column 1 has a lower back pressure than column 2 at same flow rate, so it is
possible to obtain higher flow rates with the same required air pressure on the
column 1 compared to the column 2.
For the calibration you have to measure the flow rate at different required air
pressures from 1 up to 7 bar (up to 4.2 bar with the 100 ml pump head)
120
80
Column 1
40
Column 2
0
1 2 3 4 5 6 7
Fig. 11 Typical calibration curves of the Pneumatic Pump K–1900 for two different
columns (250 ml pump head)

Easy Maintenance
12
Easy Maintenance
Changing and Cleaning the Pump Head
SOP 3 Removing the Pump Head
This SOP refers to the Pneumatic Pump K–1900.
For exchange of the pump head, or for disassembling it in order to clean valves
or replace piston seals, piston rods etc., the pump head can easily be removed.
1. Clean the pump head with a suitable cleaning reagent and then with
distilled water according to chapter „Start of Solvent Delivery“ on page
11, step 1 and 2.
If organic solvents remain in the pump head, danger of skin irritation may
exist.
2. Remove the two solvent tubings from the „Eluent inlets“, Pos. {1.2}see
Fig. 2 on page 7.
3. Loosen the two „Eluent outlets to transducer“, Pos. {1.7} and the two
„Inlets to transducer“, Pos. {2.1}.
4. Remove the two „Connection capillaries“, Pos. {1.8}, beginning with the
upper „n“-shaped one.
5. Loosen the „Inlets valve housing“, Pos. {1.5}as well as the fittings at
„Outlets valve housing“, Pos. {1.6}.
6. Using a hexagonal spanner no. 4 (4 mm), loosen just two diagonally
opposed „Head set screws“, Pos. {1.3}. Remove the screws.
7. Carefully loosen the two remaining screws, alternating from one to the
other, approx. half a turn. This prevents the pump head from tilting and
becoming damaged. Once the spring tension has been reduced, hold the
pump head firmly in one hand while removing the screws completely with
the other hand.
8. Carefully remove the pump head.
SOP 4 Removing and Checking Piston Rods
This SOP refers to the following pump heads:
100 ml stainless steel, KNAUER order number A 4029-1
250 ml stainless steel, KNAUER order number A 4021-1
500 ml stainless steel, KNAUER order number A 4038-1
1000 ml stainless steel, KNAUER order number A 4022-1
1. Remove Pump head as described in SOP „Removing the Pump Head“.
If you only wish to check the piston rods, you do not need to disassemble
the pump head any further.
2. The „Piston rods“, Pos. {3.17} in Fig. 12 on page 14 may be removed
using pliers. Take the tip of the piston using the pliers, and pull it out
carefully in a straight line.
If the rods are broken, you must check the entire pump head for damage.

13
Easy Maintenance
SOP 5 Disassembling the Pump Head
This SOP refers to the pump heads 100 ml, 250 ml, 500 ml, and 1000 ml.
3.1
3.3
3.6
3.10
3.19
3.18
3.16
3.15
3.13 3.14
3.11
3.17
3.11
3 B
3.12
3 A
3.10
3.5
3.2
3.A Pump head housing, front block 3.11 Piston seals
3.B Pump head housing, basic plate 3.12 O-shaped sealings
3.1 Piston backflushing 3.13 Distance plates
capillaries 1/16“ 3.14 Spacing bolts
3.2 Eluent inlets 3.15 Springs
3.3 Pump head set screws 3.16 Guides for springs
3.5 Inlet valve housing 3.17 Piston rods
3.6 Outlet valve housing 3.18 Retaining plate
3.10 Check valves, inlet and outlet 3.19 Retaining plate screws
Fig. 12 Explosion diagram of pump heads
1. Apply SOP 4 "Removing and Checking Piston Rods".
2. Loosen the two „Retaining plate screws“, Pos. {3.19} half a turn,
alternating from one to the other to avoid damaging the „Retaining plate“.
3. Because the two screws are very tight, it may be helpful to either clamp
the pump head or to press one of its side surfaces against a table with
one hand while loosening the screws.
4. Unscrew the two screws strictly alternating due to strong force of the
„Springs“, Pos. {3.15} behind the plate, and remove them.
5. Remove the „Retaining plate“, Pos. {3.18}.
6. You can now remove the „Guides for springs“, Pos. {3.16}, the „Springs“,
Pos. {3.15} as well as the two „Distance plates“, Pos. {3.13}.
7. Using the SW 10 spanner, loosen and remove the „Spacing bolts“, Pos.
{3.14}. These bolts are seated very tightly. Follow the tip given in step 3.
8. Lift and remove the „Pump head housing, basic plate“, Pos. {3.B}.
9. To remove the piston seals, Pos. {3.11} in the basic plate {3.B} and in the
front block {3.A}, use an appropriate tool, e.g. a screw driver and carefully
lever the four piston seals.

Easy Maintenance
14
SOP 6 Assembling the Pump Head and Exchanging Piston Seals
This SOP refers to the pump heads 100 ml, 250 ml, 500 ml, and 1000 ml.
All positions of components refer to Fig. 12 „Explosion Diagram and list of parts
of 100 ml, 250 ml, and 1000 ml pump heads“ on page 14.
1. It is recommended to exchange all four piston seals located in the pump
heads after disassembling the pump head.
Fig. 13 Open side of piston seal
2. With the open side facing downwards, carefully press the „Piston seals“,
Pos. {3.11} into the „Pump head housing, front block“, Pos. {3.A} and
„Pump head housing, basic plate“, Pos. {3.B}, making sure to keep them
straight.
3. Insert the O-ring sealings part {3.12}.
4. Put the two housing parts together, positioning the O-ring side of part
{3.B} against part {3.A}. Be sure that the backflushing capillaries are
positioned on the top side of the head - see label on front of part {3.A} for
orientation.
5. Insert the two „Spacing bolts“, Pos. {3.14}, screw them in with the hand
and tighten them as firmly as before using the SW 10 spanner.
6. Put in the two „Distance plates“, Pos. {3.13}, position the „Springs“, Pos.
{3.15} and the „Guides for springs“, Pos. {3.16}.
7. Put the „Retaining plate“, Pos. {3.18} over the „Guides for springs“, Pos.
{3.16}, with the conical bore of the plate directed outwards.
8. Insert the two „Retaining plate screws“, Pos. {3.19}, press the plate down
with one hand and tighten the screws with a screw driver, strictly
alternating due to strong force of the „Springs“, Pos. {3.15}.
9. Carefully insert the piston rods, see „Piston rods“, Pos. {3.17} without
bending or quenching the rods.
10. The „Retaining plate screws“, Pos. {3.19} must be tightened that they are
seated as securely as before.
SOP 7 Installing the Pump Head
This SOP refers to the following pump heads:
100 ml stainless steel, KNAUER order number A 4029-1
250 ml stainless steel, KNAUER order number A 4021-1
500 ml stainless steel, KNAUER order number A 4038-1
1000 ml stainless steel, KNAUER order number A 4022-1
1. Make sure that the pump head is properly assembled.
2. Position the head in a straight line onto the pump housing.
3. Tighten all four „Pump head set screws“, Pos. {3.3} a few turns by hand.
4. Alternating from one to the next, tighten two diagonally opposed screws
half a turn at a time, until the pump head is correctly seated.
5. Tighten the two other screws.
6. Make sure that all four „Pump head set screws“, Pos. {3.3} are securely
tightened.

15
Easy Maintenance
7. Install the two „Connection capillaries“, Pos. {3.8}, beginning with the
lower „s“-shaped one, and firmly tighten all fittings.
SOP 8 Cleaning and Replacing Check Valves
This SOP refers to the following pump heads:
100 ml stainless steel, KNAUER order number A 4029-1
250 ml stainless steel, KNAUER order number A 4021-1
500 ml stainless steel, KNAUER order number A 4038-1
1000 ml stainless steel, KNAUER order number A 4022-1
If the check valves become dirty they will no longer open and close correctly.
You can remove the check valves for cleaning from the pump head after
loosening and removing the „Inlet valve housing“, Pos. {3.5} and „Outlet valve
housing“, Pos. {3.6}, and then disassemble them according to the following
instructions.
1. Place the valves in a suitable cleaning solution. Use a ultrasonic bath to
clean the valve. If malfunction persists, use steps 2…5 of this SOP.
2. Using a knife or similar, carefully remove the valve seal from the housing,
see Fig. 23 on page 41.
3. Remove the valve ball out of the housing.
4. Clean the individual parts. We recommend a ultrasonic bath.
5. Assemble the check valves in reverse order, use Figure Fig. 14.
Housing Valve seal
Valve ball
Fig. 14 Components of the check valves of all pump heads available

Packing List
16
Packing List
The delivery of the Pneumatic HPLC Pump K-1900 with the 100 ml, 250 ml, 500
ml, or 1000 ml pump head (order number A50751, A50761, A50771, A50781)
comprising the items according to Table 3.
Table 3 The delivery consists of:
Peaces Description
Pump basic unit:
1 User manual
1 Coupler, to attach air pressure tube
1 Coupler socket
1 Blind fitting
Set of tools including:
1 Double open-end wrench, 13 and 17 mm
1 Double open-end wrench, 8 and 10 mm
1 Double open-end wrench, 1/4” and 5/16”
1 Screw key, 4 mm
1 Recessed head screw No. 2
Syringe set including
1 Syringe
1 Luer-Lock needle
1 0,1 m PTFE tube (ID 1,5 mm/ OD 2,1 mm)
Eluent outlet set including
1 Capillary AD 1/8”
2 Fitting screws
2 Ferrules
Set of fittings and adapters (1/8” – 1/16”) including
1 1/8” – 1/16” transforming coupler
1 Fitting screw for capillary AD 1/8” (M8x1)
1 Ferrule for capillary AD 1/8”
1 Fitting screw for capillary AD 1/16”
1 Ferrule for capillary AD 1/16”
Pump head 100 ml, order no. A 4029-1*)
1 Pump head 100 ml
4 Tube fitting screws
6 Tube ferrules
2 1 m PTFE tube (ID 3 mm/ OD 4 mm)
1 0,5 m PTFE tube (ID 1,5 mm/ OD 2,1 mm)
Pump head 250 ml, order no. A 4021-1 *)
1 Pump head 250 ml
4 Tube fitting screws
6 Tube ferrules
2 1 m PTFE tube (ID 3 mm/ OD 4 mm)
1 0,5 m PTFE tube (ID 1,5 mm/ OD 2,1 mm)
Pump head 500 ml, order no. A 4038-1 *)
1 Pump head 500 ml
4 Tube fitting screws
6 Tube ferrules
2 1 m PTFE tube (ID 3 mm/ OD 4 mm)
1 0,5 m PTFE tube (ID 1,5 mm/ OD 2,1 mm)
Pump head 1000 ml, order no. A 4022-1 *)
1 Pump head 1000 ml
2 1 m PTFE tube (ID 7 mm/ OD 9 mm)
1 0,5 m PTFE tube (ID 1,5 mm/ OD 2,1 mm)
*) Please select the appropriate pump head for your special require
ments.

17
Spare Parts and Accessories
Spare Parts and Accessories
Table 4
KNAUER
Order Number
User Manual
V 7027
Optional Pump Heads
Pump Head 100 ml incl. accessory kit
A 4029-1
Pump Head 250 ml incl. accessory kit
A 4021-1
Pump Head 500 ml incl. accessory kit
A 4025-1
Pump Head 1000 ml incl. accessory kit
A 4022-1
Spare Parts for Pump Head 100 ml
see Explosion Diagram of Pump Heads on page 14
G 0299
Check valve {3.10}
Piston rod 1/4´´ {3.17} A 0747
Set of Piston seals including
2 piston seals for 1/4´´ pistons {3.11}
1 O-shaped sealing {3.12}
A 0746
Set of Hydraulic connections including
10 tube fitting screws and 10 tube ferrules
A 2003
Inlet junction including
2 Inlet valve housings
1 T peace, fitting srcew with ferrule
1 left-hand and 1 right-hand connection tube
A 1121
Spare Parts for Pump Head 250 ml
Check valve {3.10}
A 1122
Piston rod 3/8´´ {3.17}
A 1017
Set of Piston seals including
2 piston seals for 3/8´´ pistons {3.11}
1 O-shaped sealing {3.12}
A 1046
Set of Hydraulic connections including
10 tube fitting screws and 10 tube ferrules
A 2003
Inlet junction including
2 Inlet valve housings
1 T peace, fitting srcew with ferrule
1 left-hand and 1 right-hand connection tube
A 1117
Spare Parts for Pump Head 500 ml
Check valve {3.10}
Piston rod 9/16´´ {3.17}
Set of Piston seals including
2 piston seals for 9/16´´ pistons {3.11}
1 O-shaped sealing {3.12}
A 1080
A 1478
A 1479
Spare Parts for Pump Head 1000 ml
Check valve {3.10}
Piston rod 3/4´´ {3.17}
Set of Piston seals including
2 piston seals for 3/4´´ pistons {3.11}
1 O-shaped sealing {3.12}
A 1080
A 1015
A 1077

Allgemeine Beschreibung der Pneumatischen Pumpe K–1900
18
Allgemeine Beschreibung der Pneumatischen Pumpe
K–1900
Die neue Pneumatische Pumpe K–1900 wurde für die Lösungsmittelförderung
in folgenden Einsatzgebieten entwickelt:
• Chemische Prozesse
• Säulenfüllung
• Präparative Chromatographie
Sie wird mit Druckluft angetrieben. Die Lösungsmittelförderung erfolgt mit
einstellbarem Förderdruck. Da keine elektrischen Anschlüsse notwendig sind,
kann sie auch in explosionsgeschützter Umgebung betrieben werden.
Die neue Pneumatische Pumpe K–1900 wurde für Anwendungen bei
konstantem Druck entwickelt. Sie ist zum Füllen von Hochleistungssäulen
(microbore, analytisch und präparativ) bis zu 1000 bar geeignet. Der Fluss ist
ausgezeichnet reproduzierbar, und es ist nur geringster Wartungsaufwand
erforderlich.
Die Pumpe zeichnet sich durch ein Doppelkolbensystem aus. Sie kann mit vier
verschiedenen Pumpenköpfen konfiguriert werden und deckt dadurch einen
weiten Fluss- und Druckbereich ab:
• 100 ml-Pumpenkopf, Edelstahl, bis zu 1000 bar
• 250 ml-Pumpenkopf, Edelstahl, bis zu 200 bar
• 500 ml-Pumpenkopf, Edelstahl, bis zu 100 bar
• 1000 ml-Pumpenkopf, Edelstahl, bis zu 50 bar
Die Bezeichnung der Pumpenköpfe hat historische Ursachen. Sie können die
genannten Pumpenköpfe auch mit unserer Präparativen HPLC Pumpe K-1800
verwenden. Beim Betrieb mit der pneumatischen Pumpe K–1900 arbeiten die
Kolbenantriebe mit einer höheren Frequenz und deshalb kann ein wesentlich
höherer Fluss realisiert werden, als er auf den Pumpenköpfen angegeben ist.
Die Zusammenhänge von Fluss und hydraulischem Druck sind für die
verschiedenen Pumpenköpfe der K-1900 in Tabelle 2, Technische Daten der
einzelnen Pumpenköpfe auf Seite 6 aufgelistet.
Die Pumpenköpfe können durch Lösen von nur vier Schrauben ausgetauscht
werden. Erfahrene Anwender können die Köpfe zu Reinigungszwecken oder
zum Austausch von Verschleißteilen einfachzerlegen.
Darüber hinaus steht eine am Pumpenkopf zu montierende Kühlvorrichtung
(Wärmeaustauscher) zur Verfügung. Sie ermöglicht den Betrieb der
Pneumatischen Pumpe K–1900 mit superkritischen Flüssigkeiten, wie CO2, für
Extraktionen und für die Superkritische Flüssigchromatographie.
Die Antriebseinheit der Pumpe besteht aus einem pneumatischen Kolben, der
durch Druckluft angetrieben wird. Die pneumatische Energie wird durch
Kraftübertragungshebel auf die Kolbenstangen die übertragen.

19
Hinweise zum Gebrauch des Handbuchs
Hinweise zum Gebrauch des Handbuchs
Konventionen in diesem Handbuch
Besondere Warnhinweise und Hinweise auf mögliche Probleme sind mit dem
Warnsymbol gekennzeichnet.
Ein nützlicher Tip wird in der Marginalspalte durch das Symbol
hervorgehoben.
Wichtige Hinweise werden in der Marginalspalte durch das Hinweissymbol
kenntlich gemacht.
Die Bezüge zu Details in Abbildungen im Text dieses Handbuchs werden durch
das Format wie z.B.: siehe Pos. {3} in Abb. 1 auf Seite 6 charakterisiert.
SOP's in diesem Handbuch
Die Standardarbeitsanweisungen (Standard Operating Procedures, SOP)
dieses Handbuches ermöglichen die Strukturierung zusammenhängender
Aufgaben beim Betrieb Ihrer Pneumatischen Pumpe K-1900. Sie beinhalten
schrittweise Anweisungen, die den Anwender durch alle Aufgaben führen. Sie
können gleichfalls zu Dokumentationszwecken genutzt werden. Sie können
kopiert, angewendet, unterzeichnet und abgelegt werden, um so die
Leistungsfähigkeit Ihres Gerätes zu dokumentieren.
Bitte betreiben Sie das Gerät inklusive Zubehör gemäß der SOPs in
diesem Handbuch. Andernfalls können fehlerhafte Meßergebnisse,
Beschädigungen oder gesundheitliche Beeinträchtigungen des
Anwenders eintreten, obwohl dieses Gerät außerordentlich robust und
betriebssicher ist.
Tabelle 1
SOP 1
SOP’s in diesem Handbuch
Einrichtungder Pneumatische Pumpe K-1900für die Kolbenhinterspülung
25
SOP 2
Starten der Lösungsmittelförderung
26
SOP 3
Pumpenkopf ausbauen
28
SOP 4
Kolbenstangen ausbauen und prüfen
28
SOP 5
Pumpenkopf zerlegen
29
SOP 6
Zusammenbau des Pumpenkopfes und Austausch der Kolbendichtungen
30
SOP 7
Pumpenkopf einbauen
30
SOP 8
Kugelventile reinigen und ersetzen
31

Technische Daten der Pneumatischen Pumpe K–1900
20
Technische Daten der Pneumatischen Pumpe K–1900
Tabelle 2 Technische Daten der einzelnen Pumpenköpfe
Merkmal Pumpenkopf
100 ml 250 ml
500ml
1000 ml
Maximale Flussrate (ml/min) bei
300
500
1000
2000
minimalem Gegendruck
Lösungsmittelförderung bei maximal
1000
200
100
50
zulässigem Pressluftdruck (bar)
4,2 *)
7
7
7
Kolbenhub (mm)
9,5
9,5
9,5
9,5
Hubvolumen je Zyklus (ml)
0,6
1,35
3,05
5,4
Kolbendurchmesser (mm)
6,35
9,525
14,29
19,05
Pneumatisches Umwandlungsverhältnis
1:250
1:96
1:47
1:23
*) Siehe Warnung unten.
Beim 100 ml-Pumpenkopf darf der Pressluftdruck den Wert von 4,2 bar auf
keinen Fall übersteigen. Ein höherer Druck als 4,2 bar führt zur
Beschädigung des Pumpenkopfes und der Pumpenmechanik. Diese
Schäden fallen nicht unter die Garantiegewährleistung.
Inbetriebnahme der Pneumatischen Pumpe K-1900
Auspacken
Alle KNAUER-Geräte werden ab Werk sorgfältig und sicher für den Transport
verpackt. Prüfen Sie dennoch nach dem Auspacken alle Geräteteile und das
Zubehör auf mögliche Transportschäden und machen Sie gegebenenfalls
Schadensersatzansprüche sofort beim Transportunternehmen geltend.
Bitte überprüfen Sie gemäß Abschnitt Lieferumfang auf Seite 32 das Zubehör
auf Vollständigkeit. Sollte trotz unserer sorgfältigen Ausgangskontrollen ein Teil
fehlen, wenden Sie sich bitte an unsere Serviceabteilung.
Bitte entfernen Sie die transparente Schutzfolie vom Pumpenausgang.
Die komplette Liste der verfügbaren Verschleiß- und Ersatzteile finden Sie im
Abschnitt Ersatzteile und Zubehör auf Seite 33.