Knauer WellChrom HPLC Pump K-1001 User manual

●Wissenschaftliche Gerätebau Dr. Ing. Herbert Knauer GmbH ●Hegauer Weg 38 ●D-14163 Berlin GERMANY
●Telefon: [phone] ●Fax: [phone] ●Email: [email protected] ●www.knauer.net
WellChrom
HPLC-Pump K-1001
HPLC-Pumpe K-1001
Manual / Handbuch
V1400, 06/2003

CONTENTS
Using this Manual 4
Conventions in this Manual 4
SOP´s in this manual 5
General Description 6
Setup of the HPLC Pump K–1001 7
Unpacking 7
Pump head identification 7
Front View of the HPLC Pump K–1001 8
Pump Heads and Hydraulic Connections 9
Rear Panel View of the HPLC Pump K–1001 10
Connection to Main Power 10
Control of the HPLC Pump K–1001 11
Basics for operating the HPLC-Pump K-1001 11
Power On and Selftest 11
Appearance of the Cursor 11
Menu Structure of the HPLC Pump K–1001 11
Using the Arrow keys in the SETUP Menu 12
Using the SETUP Menu 12
Using the VIEW Menu 14
Using the LINK Menu 15
Using the GLP Menu 16
Operating the HPLC Pump K–1001 17
Working Example for setting parameters 17
Using the Low Pressure Gradient Mode 18
Using the High Pressure Gradient Mode 18
Direct Control of the Pump 18
Maximum Pressure 18
Piston Backflushing 20
Programming the HPLC Pump K–1001 21
Features of Programs 21
Example for creating a program 24
Using Program Links 24
Connecting the HPLC Pump K–1001 to Other Instruments 26
Hydraulic Connections 26
Single Connection 26
Low Pressure Mixing Inlet 26
WellChrom Solvent Organizer K–1500 27
Electrical Connections 28
Using the EVENT and ANALOG/ERROR/START Sockets 28
Configuring Plug Strips 28
Using the RS232 Serial Interfaces in the KNAUER-Net 30
Easy Maintenance 32
Piston backflushing 32
Changing and Cleaning the Pump Head 32
Check valves 35
List of Display Messages 37
List of Error Codes 37
Packing List 38
Spare parts and accessories 39
Technical Data 40
Declaration of conformity 41
Guarantee statement 42

INHALT
Hinweise zum Gebrauch des Handbuchs 43
Konventionen in diesem Handbuch 43
SOP´s in diesem Handbuch 44
Allgemeine Beschreibung 45
Inbetriebnahme der HPLC-Pumpe K-1001 46
Auspacken 46
Pumpenkopf Identifikation 46
Frontansicht der HPLC-Pumpe K–1001 47
Pumpenköpfe und Flüssigkeitsanschlüsse 48
Rückseitenansicht der HPLC-Pumpe K-1001 49
Netzanschluss 49
Bedienung der HPLC-Pumpe K-1001 50
Grundsätzliches zum Betrieb der HPLC-Pumpe K-1001 50
Einschalten und Selbsttest 50
Aussehen des Cursors 50
Menüstruktur der HPLC-Pumpe K-1001 50
Verwendung der Pfeiltasten im SETUP-Menü 51
Das SETUP-Menü 52
Das VIEW-Menü 54
Das GLP-Menü 55
Das LINK-Menü 54
Bedienung der HPLC-Pumpe K-1001 56
Arbeitsbeispiel für Parametereinstellungen 56
Anwendung des Hochdruckgradienten-Modus 57
Direkte Steuerung der Pumpe 57
Druckmaximumbegrenzung 57
Kolbenhinterspülung 59
Erstellung von Programmen mit der HPLC-Pumpe K-1001 61
Eigenschaften von Programmen 61
Beispielprogramm 64
Programmverküpfungen (Links) 65
Verbindung der HPLC-Pumpe K-1001 mit weiteren Geräten 67
Flüssigkeitsverbindungen 67
Einzelanschluß 67
Niederdruck-Mischblock 67
WellChrom Solvent Organizer K–1500 68
Elektrische Verbindungen 69
Verwenden der Event- und ANALOG/ERROR/START-Anschlüsse 69
Konfektionierung der Fernsteuerkabel 69
Nutzung der seriellen RS232-Schnittstellen im KNAUER-Netz 71
Wartung durch den Anwender 73
Kolbenhinterspülung 73
Austausch und Reinigung des Pumpenkopfes 73
Kugelventile 77
Liste der Displaymeldungen 79
Liste der Error Codes 79
Lieferumfang 80
Ersatzteile und Zubehör 81
Technische Daten 82
Konformitätserklärung 83
Garantiebedingungen 84

4 Using this Manual
Using this Manual
Conventions in this Manual
Special Warnings are indicated by the marginal warning sign and printed in
bold letters.
The marginal lamp symbol indicates helpful advice’s.
Important Hints are marked by the marginal hand symbol.
Arrows in an outlined form like this example used in block diagrams, indicate an
automatic program run and change to the next line without the necessity of
manual interventions.
Arrows like this, used in block diagrams, indicate that the user is asked to press
the corresponding arrow keys
¾¿½ÀThe triangles symbolize the use of corresponding arrow keys.

Using this Manual 5
SOP´s in this manual
The Standard Operating Procedures (SOP) provided with this manual offer a
convenient way of structuring complex tasks in the operation of your WellChrom
HPLC pump K-1001. They include step-by-step instructions leading the user
through all routine tasks during operation. They can be used for documentation
purposes and be copied, applied signed, and filed in order to document the
performance of the instrument.
Please operate the instrument and all accessories according to
instructions and SOP´s in this manual. This ensures proper results and
longevity of your equipment.
Table 1 SOP´s in this manual
SOP 1 Selecting Menus 11
SOP 2 Entering Data 12
SOP 3 Selecting a Gradient Mode 13
SOP 4 Selection of correct pump head: 17
SOP 5 Selection of operation mode: 17
SOP 6 Setting the Maximum Pressure 18
SOP 7 Setting the Flow Rate 18
SOP 8 Setting the Solvent Composition 18
SOP 9 Setting Events 19
SOP 10 Start and Stop of Solvent Delivery 19
SOP 11 Purging the HPLC Pump K–1001 19
SOP 12 Preparing your HPLC Pump K–1001 for piston backflushing 20
SOP 13 Creating a Program 21
SOP 14 Execution of Programs 22
SOP 15 Modifying and Deleting Programs 23
SOP 16 Using a Wake-Up Program 23
SOP 17 Creating Program Links 24
SOP 18 Executing Program Links 25
SOP 19 Connection of eluent tubings 26
SOP 20 Fixing the Solvent Organizer K-1500 above the Pump K-1001 27
SOP 21 Connecting the Solvent Organizer K–1500 28
SOP 22 Mounting Plug Strips 29
SOP 23 Piston backflushing 32
SOP 24 Removing the Pump Head 32
SOP 25 Removing and Checking Piston Rods 33
SOP 26 Disassembling the pump head 33
SOP 27 Assembling the pump head 34
SOP 28 Installing the pump head 35
SOP 29 Replacing check valves 35
SOP 30 Cleaning check valves 36

6 General Description
General Description
The HPLC Pump K–1001 features a double-piston design with active pulsation
dampening. It is capable of delivering solvents with very low residual pulsation.
The HPLC pump K-1001 is delivered with the new inert pump heads with
ceramic inlays. Two different pump heads are available:
• Analytical pump head (0.001 – 9.999 ml/min),
• Preparative pump head (0.01 – 50 ml/min).
These heads combine the features of the older stainless steel versions with
those of the PEEK ones and replace both from now.
The new features are
• Inert, biocompatible.
• The 10 ml pump head is pressure stable up to 400 bar.
• The 50 ml pump head is still pressure stable up to 150 bar.
• The sealings are easily accessible due to the new splitted design.
Four different older pump heads can also be used with the HPLC pump K–
1001. These are the analogous stainless steel and PEEK versions respectively:
• Analytical pump head (0.001 – 9.999 ml/min), stainless steel
• Analytical pump head (0.001 – 9.999 ml/min), PEEK
• Preparative pump head (0.01 – 50 ml/min), stainless steel
• Preparative pump head (0.01 – 50 ml/min), PEEK
Pump heads can be interchanged by loosening only four screws. Experienced
users can disassemble the heads for simple maintenance tasks like cleaning,
changing piston seals etc. The easy-to-learn user interface of the HPLC Pump
K–1001 provides access to the integrated software. It supports quaternary high
pres-sure gradient systems via a master / slave configuration, where up to three
additional K–1001 pumps can be controlled.
The optional Solvent Organizer K–1500 adds support for quaternary low
pressure gradients. Its internal mixing valves, available in either stainless steel
or PEEK, can be controlled by the HPLC Pump K–1001.
Most KNAUER Software Packages, like ChromGate®or EuroChrom®, allow for
digital remote control of any configuration of KNAUER solvent delivery systems,
enabling full and transparent integration of the HPLC Pump K–1001 pump into a
wide range of chromatographic systems.

Setup of the HPLC Pump K–1001 7
Setup of the HPLC Pump K–1001
Unpacking
Unpack the instrument and check pump and accessories for any damage due to
shipping. Place any claims referring to damage at the transportation company
responsible for shipping.
Please check that the HPLC Pump K–1001 is complete, use section „Packing
List“. Should, in spite of our thorough control procedures, something be missing,
please contact our service department.
Pump head identification
The pump heads are labeled on the front side indicating the ceramics inlay
version and the maximum flow rate.
The labels of older stainless steel and PEEK versions respectively show the
maximum flow rate only.
These older pump heads can easily be identified by their color:
black: ➜biocompatible PEEK-version
metallic: ➜stainless steel version
Maximum flow rate, 10 or 50 ml/min is indicated on a label on the front of the
pump head.

8 Setup of the HPLC Pump K–1001
Front View of the HPLC Pump K–1001
{1} Display information
{2} Foil key area
{3} Pump head
{4} Pressure transduce
r
Fig. 1 Front Panel of the HPLC Pump K–1001
Fig. 1 shows the front view of the HPLC Pump K–1001. For additional
information on connecting the pump head see section „Connecting the HPLC
Pump K–1001 to other Instruments“ on page 26 and Fig. 19 „Pump head:
Connection of eluent tubings“ on page 26.
{1.1} {1.2} {1.3} {1.4} {1.5}
{1.9} {2.3} {2.4}
{1.6}
{1.7}
{1.8}
{1.12}
{1.11}
{1.10}
{2.1}
{2.1}
1 Display information 1.9 elapsed Run Time
1.1 Program to be loaded 1.10 Status of program or Pump
1.2 Program to be edited 1.11 Go to the VIEW Menu
1.3 Flow rate 1.12 Go to the LINK Menu
1.4 |preset maximum| or
measured system pressure 2 Foil Key Arear
1.5 State of Events 2.1 Pump START / STOP
1.6 Go to the SETUP Menu 2.2 Purge START / STOP
1.7 Go to the GLP Menu 2.3 Numeric Key pad
1.8 Gradient mixing ratios 2.4 Arrow keys
Fig. 2 Display information and foil key area in detail

Setup of the HPLC Pump K–1001 9
The display shows information about the current status of the pump, such as
the flow rate, the measured pressure or the current status of the running
program. It serves as a control instrument when entering data.
Pumping can be started or stopped by pressing the key START/STOP, see
{2.1}, using the preset values. The foil key PURGE, see {2.2}, allows immediate
access to the purging function of the pump.
You can use the keys for entering data and control the execution of programs.
Detailed information is given in see „Control of the HPLC Pump K–1001“ on
page 11 and „Programing the HPLC Pump K–1001“ on page 21.
Pump Heads and Hydraulic Connections
All hydraulic connections are on the front panel of the HPLC Pump K–1001, see
Abb. 3. For connecting eluents to the pump head see section „Connecting the
HPLC Pump K–1001 to other Instruments“ and Fig. 19 on page 26.
{3.2}
{3.6}
{3.3}
{3.4}
{3.5}
{4.4}
{4.3}
{4.2}
{4.1}
{3.7}
{3.1}
3 Pump Head 4 Transducer with Purge Valve
3.1 Piston backflushing capillaries 4.1 Inlets to tranducer
3.2
3.3
Eluent inlet
Head set screws
4.2 De-areation capillary,
(Purging outlet)
3.4 Label indicating the maximum
flow rate
4.3 De-areation screw for opening
purge valve
3.5 Inlet valve housing 4.4 Eluent outlet to column
3.6 Outlet valve housing
3.7 Eluent outlet to tranducer
Fig. 3 Pump Head and tranducer with Purge valve

10 Setup of the HPLC Pump K–1001
Rear Panel View of the HPLC Pump K–1001
{5.10} {5.1} {5.2} {5.3}
{5.4}
{5.5}
{5.6}
{5.7}
{5.9}
{5.8}
5 Rear panel elements
5.1 Fuse compartment 5.6 Ventilator
5.2 Power connector 5.7 Serial number
5.3 ON/OFF switch 5.8 Terminal strip REMOTE con-
nections
5.4 RS-232 interfaces 5.9 Terminal strip REMOTE con-
nections
5.5 Socket prepared for Ethernet
connection
5.10 Plug for interconnection of
Solvent Organizer K-1500
Fig. 4 Rear Panel of the HPLC Pump K–1001
Connection to Main Power
The WellChrom HPLC Pump K-1001is equipped with an universal power supply
for input voltages ranging from 90 to 260 Volts AC. A manual setting of the
supply voltage is not required.
Make sure that the main power supply is properly grounded and a correct
three-pronged power cable is used. Connecting the instrument to faulty
power sockets can cause damages.
Connect the power cable to the socket on the rear of the instrument, and switch
on the device with the ON/OFF key, see {5.2} and {5.3} in Fig. 4.

Control of the HPLC Pump K–1001 11
Control of the HPLC Pump K–1001
Basics for operating the HPLC-Pump K-1001
Power On and Selftest
On power on the instrument displays HPLC PUMP MAXI-STAR, and the
VERSION of the software, e.g. 6.0X. The instrument performs a self test,
including firmware validation and tests of RAM and motor unit. After completion
the message SYSTEMTEST OK indicates that the pump is ready for operation
and the ID of the last active program is displayed.
Never run the HPLC Pump K–1001 without liquid in the pump head or in
the piston backflushing compartment. For piston backflushing, follow the
instructions given in chapter „Piston Backflushing“ on page 32. Operating
the pump without solvents may lead to damage of the pump seals.
Appearance of the Cursor
You can use the yellow arrow keys on the foil keypad for positioning the blinking
cursor on any field of the menu screen.
The cursor appears as underscore character while entering data, see Fig. 5.
Use the numeric key pad for data input, Pos. {2.3} in Fig. 2, and refer to SOP 2
„Entering Data“ on page 12.
Fig. 5 Blinking Cursor Cursor during data input
Menu Structure of the HPLC Pump K–1001
The display of the HPLC Pump K–1001 uses one main screen with status
information of the instrument. From the main screen you can access four
additional screens for display of parameters.
By default, the HPLC Pump K–1001 displays the parameters of the first
program line of the currently active program.
The center part of Fig. 6 „Menu Structure of the HPLC Pump K–1001“ shows an
example of the main screen.
01 -- 100.0 000 0 0 0 0 0 0 0 0
OFF 000.00
Load Edit Prog Flow Pressure Events
Prog Clear 0 [ml/min] [0.1 Mpa] off 0 on 1 2
Hold 0 Time %A %B %C %D
Run 1 [min]
Setup
GLP
Link
View
LINK Menu
VIEW Menu
SETUP Menu
GLP Menu
Fig. 6 Menu structure of the HPLC Pump K-1001
SOP 1 Selecting Menus
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X. Use this
SOP for selecting menus in your HPLC Pump K–1001.
You can access the four menus of the HPLC Pump K–1001 with the following
procedure:

12 Control of the HPLC Pump K–1001
1. Place the cursor in a field in one of the four corners of the main screen,
e.g. on OFF in the lower left corner of the main menu.
2. Depending on the selected corner, press the corresponding left or right
arrow key longer than 2 seconds. In the example above selection of key
½will activate the VIEW Menu on the display.
3. Select the rhombus in the lower left corner of the display if you want to
access additional pages of the selected menu. You can access all menu
pages by scrolling using Àor ¿arrow keys.
4. Select the rhombus and use ¾or ½to return to the main screen.
Links: 21 ___ ___ ___ ___
(21 . . 29) ___ ___ ___ ___
Load Edit Prog Flow Pressure Events
Prog Clear 0 [ml/min] [0.1 Mpa] off 0 on 1 2
Hold 0 Time %A %B %C %D
Run 1 [min]
Setup
GLP
Link
View
Raute
Fig. 7 Example of a multi-page menu: VIEW menu
Menus without rhombus are single page menus.
SOP 2 Entering Data
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X. Use this
SOP for entering parameters and program steps in your HPLC Pump K–1001.
1. Place the cursor at the desired position of the main screen.
2. Press a numeric key to start data input. The appearance of the cursor
changes to a underline character, see Fig. 5 on page 11.
3. Press numeric keys for entering desired numeric values. Wrong entries
can simply be overwritten by continuing the entry.
4. Confirm value by pressing any of the arrow keys. This finishes data entry
mode and moves the cursor to the next field according to the arrow key
direction.
5. You can modify and delete data according to SOP 15 „Modifying and
Deleting Programs“ on page 23.
Using the Arrow keys in the SETUP Menu
Fig. 8 „Structure of the SETUP Menu“ on page 13 gives a complete overview of
the Setup Menu.
You can use ¾for accessing menus.
You can use ¿and Àfor scrolling through the list of parameters of any
individual menu item.
Using the SETUP Menu
The SETUP menu of the HPLC Pump K–1001 enables access to the following
features and functions:
GRADIENT MODE: The HPLC Pump K–1001 is capable of isocratic (none),
high pressure gradient (HPG) and low pressure gradient (LPG) operation.
You can choose the mode of operation:

Control of the HPLC Pump K–1001 13
Fig. 8 Structure of the SETUP menu
SOP 3 Selecting a Gradient Mode
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X. Use this
SOP for selecting a gradient mode.
1. Select „GRADIENT MODE“ in the SETUP Menu.
2. Select the desired mode of operation.
3. If you select either high or low pressure gradient (i. e. HPG or LPG) you
need to specify the channels of used solvents:
Low Pressure Gradient: Choose valves A to D
High Pressure Gradient: Choose pump names HPG A to HPG D
4. Move to the second line by pressing ¾and choose valves A to D (for
LPG mode) or pumps actually present (for HPG mode) on the
corresponding positions by pressing ¿or À. This selection will apply for
programs and define the number and names of solvents used in any
gradient.
Main menu

14 Control of the HPLC Pump K–1001
If after leaving this item over the rhombus the message Conflict with existing
programs is displayed, this means that programs with different valve or pump
configuration already exist. In this case, either delete these programs according
to SOP 15 "Modifying and Deleting Programs" on page 23 or change your
valve or pump configuration correspondingly.
Fig. 13 on page 17 gives a detailed example for gradient mode selection.
PUMP HEAD: You can select the type of pump head installed in your pump.
Selection of any pump head sets specific flow rate calculating data as well as
maximum system pressures with respect to its specifications. The according
maximum pressure applies to all modes of operation and cannot be exceeded.
The nowadays used pump heads with ceramic inlays correspond to the
steel versions. Please select the steel pump head of the size in use!
PRESSURE AUTOZERO: You can make an offset correction for the pressure
display. Open the purge valve by turning the de-aeration screw , see {4.3} in
Fig. 3 on page 9, one turn to the left. Select menu with ¾and start autozero
routine by pressing ¿or À. Offset correction is done automatically.
FLOW: You can enter a correction constant for the selected flow rate in a
preset range [4000 - 6000] depending on the type of pump head. This
correction changes the number of pump cycles at a selected flow rate. This
option allows for balancing different compressibility of solvents.
Only trained users should change that setting! Select menu with ¾, position
cursor on desired numeric field and adjust values with ¿or À.
You have no access to the correction parameter, see Fig. 8. These can be
accessed by authorized maintenance personnel only.
PRESSURE LIMITS: Allows to set an individual minimum and maximum system
pressure. The pump will switch off automatically if the system pressure exceeds
limits. If minimum pressure is set to 0, no check of the minimum pressure is
carried out. The absolute maximum pressure is limited by the pump head
chosen and by the flow rate selected for this pump head. The detailed pressure
limits are given in the section „Maximum Pressure“ on page 18.
The specified maximum pressure applies to all modes of operation and
cannot be exceeded. Units: 0.1 MPa (= bar).
START INPUT: If the START INPUT is set to ENABLED, a program can be
started by an external digital signal from the START IN input on the rear side of
the pump, see chapter „Connecting the Analog/error/start Socket“ on page 28.
The setting STOP Pump will cause the pump to stop pumping, if an external
digital signal is applied on the START IN input. The setting START Pump
enables to start pumping by using an external signal.
If the START INPUT is set to START Pump or STOP Pump, no program runs
can be started and network control is inactivated.
DATE/TIME: You can use this menu for setting the system date and time of
your HPLC Pump K–1001.
Using the VIEW Menu
The VIEW menu lists existing programs and program links.
An example is given in Fig. 9: Programs 1, 2, 3, 4, 11 and link 21 have been
created.

Control of the HPLC Pump K–1001 15
Fig. 9 The VIEW-menu with pPrograms 1-4, 11 and link 21
Using the LINK Menu
The LINK menu reports status information of the currently active program links.
It is activated only if a link is running.
Fig. 10 LINK-menu without running link
If a link is active, the menu shows the following structure:
Fig. 11 LINK-menu with running link
In case a link is running, the first line of the menu gives the current status of the
links. The field R-xx shows the number of program runs still to be performed.
The value of R-xx decreases according to the number of remaining runs.
The second line shows the next step of the link to be performed. Up and down
arrows at the right hand side of the display indicate existence and position of
additional program steps.
Main menu
Main menu
Main menu

16 Control of the HPLC Pump K–1001
Using the GLP Menu
The GLP menu reports statistical data for vital parameters of the HPLC Pump
K–1001. Fig. 12 gives an overview of the GLP menu.
Fig. 12 Structure of the GLP-mMenu
For an overview of error codes refer to chapter „List of Display Messages“ on
page 37.
Main menu

Operating the HPLC Pump K–1001 17
Operating the HPLC Pump K–1001
You need to setup your pump prior to operation. Use the information in sections
„Menu Structure of the HPLC Pump K–1001“ on page 11,
„Using the SETUP Menu“ on page 12,
Working Example for setting parameters
For your convenience, this example points out how to configure a binary low
pressure gradient using valves A and B.
This example may not comply with your needs and is given for
convenience only. You need to select parameters that meet your
requirements. Using this example without proper adjustment of
parameters may lead to damage of your equipment.
Enter the SETUP menu, see Fig. 8 on page 13.
SOP 4 Selection of correct pump head:
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Select menu PUMP HEAD by pressingÀ
2. Use ½or ¾to scroll through the list of pump heads until the installed type
of pump appears, e.g. 10 ml, steel. Selection of a pump head
automatically adjusts proper absolute maximum system pressure.
3. Press ½to confirm selection and return to the rhombus.
SOP 5 Selection of operation mode:
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Return to page GRADIENT MODE by pressing ¿.
2. Press ¾and then ¿to scroll through the gradient mode options until
LPG appears.
3. Use ¾to position cursor in the second line.
4. Select ON or -- (off) at positions A, B, C and D for the solvent channels in
either mode you wish to use. The number of ONs selected refers to all
programs in the pumps memory.
5. At any position you can use ¿or Àto select ON.
6. Position cursor on next position, e. g. B and select ON .
7. Select three times ¾to position cursor at the rhombus.
8. The display should appear as shown in Fig. 13.
9. Leave this menu page with ½to return to the main menu.
GRADIENT MODE: LPG
ON ON -- --
Load Edit Prog Flow Pressure Events
Prog Clear 0 [ml/min] [0.1 Mpa] off 0 on 1 2
Hold 0 Time %A %B %C %D
Run 1 [min]
Setup
GLP
Link
View
Fig. 13 Example for a gradient setup with solvents A and B in all programs

18 Operating the HPLC Pump K–1001
Using the Low Pressure Gradient Mode
Using the High Pressure Gradient Mode
If you want to control up to three additional HPLC Pumps K–1001 for a high
pressure gradient, you can connect the appropriate number of pumps using the
serial RS232 interfaces according to „Using the RS232 Serial Interfaces in the
KNAUER-Net“ on page 30.
Activate „GRADIENT MODE“ in the SETUP menu, see „Using the SETUP
Menu“ on page 12 and select individual, unique names from HPG A to HPG D
for all pumps connected. The number of pumps in your HPG system can be
selected by using the ON option in the second line. Only the pumps selected
with ON will be used for calculating the percentage composition in a program.
Every pump automatically takes control over all pumps connected, if execution
of a program is started on that pump.
Direct Control of the Pump
If you want to use the Direct Control Mode you have to specify:
Maximum system pressure
Flow Rate and
Solvent Composition if working in LPG or HPG gradient mode
Maximum Pressure
In addition to the maximum pressure specified in the setup menu, see „Using
the SETUP menu“ on page 12 by either the pump head or the global pressure
limit, you can specify a maximum system pressure for the currently active mode
of operation or the currently active program.
SOP 6 Setting the Maximum Pressure
In addition to the maximum pressure specified in the SETUP menu, either by
the pump head or by the individually set pressure limit, you can specify a
different, lower system pressure for the currently active program.
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Move the cursor to the parameter PRESSURE, see {1.4} in Fig. 2 on
page 8.
2. Type in the desired value for the maximum pressure. The selected value
is marked with two vertical lines which indicate that this is a programmed,
not a measured value.
3. Press any arrow key for confirmation.
SOP 7 Setting the Flow Rate
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X. Use this
SOP for setting the correct flow rate.
1. Position cursor on field FLOW, see {1.3} in Fig. 2 on page 8.
2. Enter the desired flow rate with the numeric keys.
3. Press any arrow key for confirmation.
SOP 8 Setting the Solvent Composition
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X, when
working in any gradient mode.
1. Move the cursor to the field % A, see {1.8} in Fig. 2 on page 8.

Operating the HPLC Pump K–1001 19
2. Type in the desired percentage of eluent A. The value in the right hand
adjacent field next to your input complements the value to give a total of
100%.
3. Press any arrow key for confirmation.
SOP 9 Setting Events
Events are defined as states or changes of states of the electrical contacts of
the Events socket of the instrument. You can either open or close any of the
contacts or use a 500 ms puls. Some times the manual control may be usefull,
e.g. for test purposes. For connection of cables use sections „Electrical
Connections“ on page 27
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Position the cursor at the event field.
2. Start entry of events by pressing 0, 1, 2 or 3 for the first event. After every
entry, the underlined cursor jumps one position to the right. The following
table gives the meaning of the possible entries:
0 — Off
1 — On
2 — Pulse
3 — No change with respect to previous status
3. Finish entry of events by pressing any arrow key.
Fig. 14 Relations between event prgramming and event termial strip
SOP 10 Start and Stop of Solvent Delivery
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.
1. Apply SOP 7 „Setting the Flow Rate“ on page 18.
2. Use the START/STOP Key, see Pos. {2.1} in Fig. 2 on page 8, to start
and stop solvent delivery.
SOP 11 Purging the HPLC Pump K–1001
This SOP applies to the HPLC Pump K–1001 Firmware Revision 6.0X.

20 Operating the HPLC Pump K–1001
Open the „De-aeration screw for opening purge valve“, Pos. {4.3} prior to
starting the purging function to avoid damaging your column system.
1. Purging without opening the purging outlet may cause damage to your
column and system.
2. Press purge key {2.2}, see Fig. 2 on page 8, for start and stop of purging.
In LPG mode, the pump is purged with the currently active solvent
composition.
3. Select a flow rate for purging, see SOP 7 „Setting the Flow Rate“ on
page 18.
4. In LPG mode, purging can be performed either in mode MIX, using the
currently active solvent composition or separately for each chammel A to
D. Select the desired mode by positioning the cursor on the appropriate
positions MIX, A, B, C or D.
Piston Backflushing
Backflushing the piston removes traces of salt and other decontaminants from
the backside of the pistons.
Especially if you use saline solvents or buffer solution we highly recommend
that you continuously backflush in order to prevent crystallization which can
damage your piston seals.
For best protection and error free long time operation of the pump head it is
recommended always to backflush the piston with an appropriate solvent
(mostly water) according to the following SOP.
SOP 12 Preparing your HPLC Pump K–1001 for piston backflushing
This SOP applies to the HPLC Pump K–1001
1. Push a 1/16" ID tube over both piston backflushing capillaries.
Fig. 15 Piston backflushing capillaries
2. Place one low end of the tubing in a flask.
3. Fill the priming syringe with the backflushing liquid.
4. Connect the syringe with the other tubing.
5. Press liquid through the pump head, until it flows without any air bubbles
into the container.
6. Remove tubings from capillary opening.
If you want operation with continuous backflushing you can attach two
containers of rinsing liquid instead of the priming syringe. The containers should
be positioned that one container is located higher than the other, thus ensuring
liquid flow through the pump head without any assistance.
When not backflushing continuously, we recommend to connect both capillary
openings with a tubing to prevent vaporization of solvents and drying out of the
piston chamber.