Labnet I5311-DS User manual

Labnet Incubator Shaker
I5311-DS and I-5311-DS-230V
Instruction Manual
PO Box 841 Woodbridge, NJ 07095 USA
Phone: [phone] Fax: [phone]
www.labnetlink.com
Copyright July 2004

CONTENTS OF THIS USERS MANUAL
GraphicSymbols page 1 Controls& Calibration page 3
SafetyPrecautions page 1 OperatingtheI-5311-DS page 5
Introduction page 2 CareandMaintenance page 5
Specifications page 2 TroubleshootingGuide page 6
Unpacking page 2 TechnicalSupport/Service page 6
Installation page 3 Accessories page 7
GRAPHIC SYMBOLS
Your Labnet I-5311-DS incubatorshakerusesinternationallyaccepted graphic symbols to
helpconveyinformationtotheuserandtocall the users attention to important safety precau-
tionsandguides for using thisequipment.
indicatesthatusershouldconsultmanualfurtherdescriptionordiscussion.
IndicatesAC“PowerOn” Indicates“Heating”
Indicates “Degrees Centigrade” Indicates “Temperature”
Indicates Potential “Shock Hazard” Indicates Protective “Earthground”
SAFETY PRECAUTIONS
Use of this product in any manner not specified by the manufacturer may impair the
safety protection provided by the equipment and may result in physical damage and/
or personal injury. Please read all operating instructions in this manual prior to use of
this equipment.
1. Donotoperatethisunitinanexplosiveorflammableenvironment.
2. Donotincubateorshakeflammableorexplosive materials or highlyreactive chemicals.
3. Lifting / Handling: These unitsare heavy andcare should betaken to use appropriate
liftingdevices. Unitsshouldonly be lifted fromtheirbottom surfaces and notbydoors,
handlesorknobs.
4. Leveling: Theunitmustsit level and solidlyonthefour leveling feet.
5. Load testing: Test all loadsto beshaken under observation to insureload and unit
stability.
6. Units are not stackable. Donot stack oneunit on top of another unit.

INTRODUCTION
Thismanualcoversthespecifications,operationanduseoftheLabnetI-5311-DSshakingincuba-
toranditsaccessories.PleasepayspecialattentiontotheSafetyPrecautionssectioninthis
manual.
TheI-5311-DSshakingincubatorfromLabnetInternationalprovidesanextremelystabletempera-
tureenvironment,andanaggressiveshakingmotionforthemixingofmaterialsforcellculture
growthatambientandaboveambienttemperatures.
TheI-5311-DSshakingincubatorusesmicroprocessorcontrolsandmechanicalconvectionto
maintainastabletemperatureenvironmentandtoachievefastchambertemperaturerecovery
afteradooropening. Theincubatorinteriorisconstructedfromstainlesssteelforcorrosionresis-
tanceandeasyclean-upofaccidentalspills. Allincubatordoorshaveanintegralglasswindowand
theincubatorhasalighttoallowobservationofsampleswithoutopeningthedoor.Eachdooris
fullythermalgasketed.
TheI-5311-DSshakingincubatorcomeswithoneremovablefullshelfandfourlarge,rubberpad-
dedadjustablefeetforlevelingtheunit. Additionalhalfandfullshelvesmaybepurchased. TheI-
5311-DSshakingmechanismsupportsawiderangeofoptionalrackandflaskplatformswhichare
userchangeable.
SPECIFICATIONS
Chamber temperature range Ambient +5C to 70C (Ambient +10°C with shaker on)
Accuracy / Uniformity @37°C +/-0.3C / +/-0.5C
Temperature accuracy +/-0.2C
TemperatureController Microprocessor - User Calibratable
Over temperature safety Independentlysettable
Shaker speed 20 to 300 rpm
Shakerorbit 19mm
Shakermax load 7.4kg*
Shaker timer Continuous (Hld) or 30 seconds to 99 minutes 50 seconds
Timer increments 30 seconds to 9 min 59 seconds in 1 second steps
10.0 minutes to 99 min 50 seconds in 10 second increments
Interiorelectricaloutlet 1 amp
Chamber volume 2.5 cu ft / 70 liters
Exterior dimensions w x d x h 574 x 544 x 644mm
Chamber dimensions w x d x h 442 x 396 x 406mm
Electrical Ratings and Unit Size
I-5311-DS 120V, 50/60Hz, 6 amps
I-5311-DS-230V 230V, 50/60Hz, 4 amps
*Maxload is4x2 literflaskswith 400mlliquidin each flask
UNPACKING
UponreceiptofyourI-5311-DSshakingincubator,examinethecartonandunitfordamages. Ifshipping
damagehasoccurred,aclaimmustbefiledwiththecarrier. Thecarrierisresponsiblefor correcting
shippingdamages. Saveall packaging untiltheunit has beenshown to operate properlyto
yoursatisfaction. Carefullyremovetheunitfromthecartonandshippingpallet.

Thepackageshouldinclude:
I-5311-DSShakingIncubator Instructionmanual
1fullshelf Power cord (both EU and UK cords in
2shelfbrackets 230V models)
4adjustablefeet WarrantyCard
CompleteandreturnthewarrantycardtoregisteryournewI-5311-DSShaking Incubator
INSTALLATION
InstallthefouradjustablefeetandlocateShakingIncubatoronastable,flat,verysolidsurface
nearagroundedelectrical outlet. The location selectedshouldbeout of direct sunlightand
awayfromheat producing sourcesor hot or coldair drafts. Atleast5cmventilation clearance
isrequiredaroundall sides of theincubator. Levelthe incubator using thefouradjustablefeet.
Clockwiserotationof a foot raises the incubator. Plug in theunit to aproperly rated and
groundedelectricaloutletandtheunitwillbereadyforuse. Installtheoptionalplatform you
haveselectedbyplugging in the fourbottomprotrusions. Larger flask platforms may require
beingscrewclamped to theshakingmechanism.
Theshelfmaybeinstalledwhenshakingflasks1literorsmaller. However, the shelf severely
limitstheilluminationfromthechamber light and the shelf maypossiblyrattleunderaggressive
shakingconditions.
CONTROLS & CALIBRATION
ThecontrolsfortheI5311-DSShakerIncubatorincludepowertotheoven,temperaturesetting,
safetytemperatureovershoot setting and shakerspeed and shaking time. Inadditionthere is
adoor-openshakershut-offswitchand a chamber light switch.
- Main Power Switch - Thisswitch(illuminated GREEN when on)turnsthe power to theunit
OnandOff.
-Temperature Controller and Temperature Set – The controller has a 3-digit display for
displaying chambertemperature or setpoint information. UP andDown arrow pads areused
tochange the set pointand controller modeof operation. To enter theset point modeof
operation,press either the UPor DOWN arrowpad one time.The display willstart to blink,
goingfrom bright to dim. Whileblinking, the displayis showing theset point.Tochange the set
point,use the UPand DOWN arrow pads. If the arrow pads arenot pressed forfive (5) sec-
onds,thedisplay will stop blinkingandwill read the chambertemperature. Aftersettingtem-
perature,allowatleast one hour forthechambertemperature to stabilize and24hoursfor
optimumstabilization.
-Calibration-TheShakingIncubator is calibratedat 37C atthefactory. The unit can bere-
calibratedafterthechamber temperature has stabilizedatthe set point forseveralhours.
Suspendacertified reference thermometerin the chamber. Comparetheunitsdisplayto the
referencethermometer.Ifthereisanunacceptabledifference,putthecontrollerintocalibration
modeby pressing boththe UP andDOWN arrow pads at the same time until the two outside
decimalpoints begin to flash. While the decimalpoints are flashing,the display canbe cali-
bratedtomatch the reference thermometerbypressing the UP orDOWNarrow pads until the
displayreadsthe correct value.Allowtheshaker incubator to stabilizeagain,andrecalibrateif
necessary.

-Heating Indicator-Thisindicatorwillilluminate GREEN when the controlleriscallingforheat
fromtheheater. Thisindicatorwillbeoncontinuously while the oven heatsuptothesettem-
peratureandwillthencycleonand off at the set temperature.
-Safety Thermostat-Thesafetythermostatlocatedonthe back of the unitismanuallysetand
completelyindependantoftheMainTermperatureController. The safety gurads against any
failureoftheMainController that would allow temperaturetorisepastthe safety set point. If
thetemperaturerisesto the safety set point,theSafetytakes control of the heatingelement
andallowscontinued use of theincubatoruntil the problem canberesolved or service canbe
arranged.
-Safety Indicator-ThisindicatorwilillimunateREDwhen the Safety Thermostat isactivated.
Undernormaloperatingconditionsthis indicator should never beon.
-Chamber Light Switch-Thisswitchturns the chamber lightonand off when powerison.
-SHAKER CONTROLS-The shaker controls are also microprocessor based.
-RUN lamp-Thislampilluminateswhentheshakerisstartedand stays illuminated when the
shakerisrunning.
-Timer lamp-Thislamp is illuminated whentheshaker display isinthe time displaymode
countingdowntheremainingruntimeorshowingthesetruntime.
-RPM lamp-Thislamp illuminates when theshakerdisplay is speed displaymodeshowing the
currentshakingspeed or showing theset shaking speed.
-Stop / Start-Thispad starts and stopsthe shaking mechanism andcauses the RUN lampto
beilluminated.
-Push to Shift / Turn Set Knob- This encoderprovides the followingmultiple functions:
•Itincreases and decreasesthe shaker speedsetting in 1 rpm increments from 20 rpm
to 300 rpm.
•Itincreases and decreasesthe run timesetting from 30seconds to 99minutes and 50
secondsand HOLD (Hld) forcontinuous operation. Timeis changed in 1second
incrementsto10minutesandthen10secondincrementsthereafter.
•Itshifts the shakerdisplay from timedisplay to speed display and back
-Shaker Display- This three digit display shows the shaker speed setting or shaker time
settingorremainingshakerruntimewhile counting down. It also shows:
•Hld -Whentheshakerissetforcontinuousun-timedrunning
•Opn -Whentheincubator door has beenopened,automatically stopping the shaking
motion(notethetimercontinues to count down butisnotdisplayed)
•End -When a timedshaking run hasended
•F60 / F50 -When the shakingincubator is firstpowered up indicatingthe line
frequencyofthepowerline.
-Door Open Switch - Thisswitch senses when the incubator shakerdoor is openand sends
asignalto the microprocessorwhichcauses the shaker tostop running as asafety measure.
However, inatimedrun, the timer willcontinuetocount down when the doorisopen. Closing
thedoorrestartstheshaking motion.

OPERATING THE LABNET I-5311-DS INCUBATOR SHAKER
Asadvisedin the installationsection,it is veryimportantthat the unitbelevel and placedon a
verysolid surface. When loading theplatforms, it isalso important to loadthese in abalanced
mannerortheunitcan vibrate and possibly walk. Shakingunbalancedloadsfor extensive
periodsmay also damagethe shaker.
Beforepoweringuptheunit,youshould first set the mechanical SafetyThermostatontheback
oftheunittoa temperature just aboveyourintendedoperatingtemperature or at max (setting
of10) this safetyis not needed.
Afteryoufirstpoweruptheunit you should notice the shaker displayfirstshowingdashesthen
thepowerinputline frequency (F60 or F50for60Hz or 50Hz) and thetemperaturecontroller
displayshowingsomerandom numbers and dotsblinking.
Theshakerdisplayandtemperaturedisplayshouldthensettle down with the shaker display
showingthelasttimethat was set (orHLd)forcontinuousoperation, and the temperature
displayshowingthechambertemperature.
Youmaynowsetthe desired operatingtemperature,the desired shakingspeedand the
desiredshakingtime (see Controls sectionof this manual). It isrecommended that you testall
loadsto be shaken aslow speed underobservation and then graduallyraise the speedto
insuretheload and unit arestable. The unit should alsobe allowed to equilibrateto the set
temperaturebeforeusing.
Oncethe temperature, shakingspeed and shakingtime are set,use the Stop/ Startpadto
initiateshaking. When shaking in the timed mode, theword End willbe displayed onthe
shakerdisplaywhen the shakingisdone. In timed modetheword Hld (hold)willbe displayed
ontheshaker display. The user may switchthe shaker display toshowthe shaking speed by
pressingthe“PushtoShift” knob.
Theusercanobservetheload being shaken through theglasswindowandcanalso turn on
the chamber light for additional visibility. The chamber light should not be left on for an
extended period as it adds heat to the chamber and may cause the chamber tempera-
ture to rise above the set point.
Theusermay open thechamberdoor at anytimeto gain accessthechamber.Ifthe shaker is
runningwhenthedoor is open, theshakerpowerwill be immediately cutcausingtheshaker to
slowandstop. Theshaker display will showOpnfor open. Shakingwillautomaticallyresume
whenthe door isclosed if thereis still timeremaining on theshaker run. Note: The clock
continuestocountdownwhenthedoor is open even though the shakerhasstopped.
CARE & MAINTENANCE
Noroutinemaintenanceisrequires for the electrical ormechanicalcomponentsofthe unit. The
incubatorexterior,interiorandshaking platform should be wipeddownperiodically with a soft
dampcloth with mild soap.Do not usechlorine-based bleach orabrasives. Any spills in the
incubator and/orontheshakingplatformsshouldbecleaned upimmediately. Be sure to
disconnect the power cord before cleaning or decontaminating the oven.

TROUBLESHOOTING GUIDE
PROBLEM POSSIBLE SOLUTION
Ovenwillnotpower up or not heat Checkpowercord,outlet and unit circuit breaker
Shakingplatformwillnot operate Check timer set to Hld or a time setting
Checkthatnothingis blocking shaker platform
PressshakerSTOP/STARTbutton
Temperaturetoohigh Checksetpoint and readjust ifnecessary
Checkcalibration
Chambertemperature goes above set Normal operation in initial heat up or ifdoor
point and settles back to set point opened for a long period
Temperaturewillnotremainstable Check that set point is at least 5C above ambient
or display shows “LO” (or10°C above ambientwhen shaker ison) which is
minimumoperatingpoint
Indicatedtemperatureisunstable Aslightvariationof +/-0.1C is normal. Largerfluctua-
tionsmaybe ambient variationsfrom drafts, door
openingandclosing, a fan obstructionorfailure or
electricalnoisefromRFI(motors,etc.)
Temperatureistoolow Ifdoorhasopened,unitmaynothaverecoveredyet.
Confirmtemperaturesetpoint.
Safetythermostatsettoolow.
Unitwillnotheatabovetemperature Confirmsetpoint.Checktemperatureofchamber
thatisbelowsetpoint withathermometerandrecalibrateifneeded.
Temperaturedisplayandreference Besurethatunithasbeenallowedtostabilizefor
thermometerdonotmatch 1hour. Thermometershouldbesuspendedin
thechamberandnottouchinganysurface.Only
certifiedreferencethermometersshouldbeused.
Cannotadjustsetpointorcalibration Turnunitofffor5secondstoreset. Ifproblem
ordisplaypersistscallservice persists,callforservice.
Unitcalibratedatonetemperature Thiscanbeanormalconditioniftemperaturesorload
andnotatanother varywidely.Forbestaccuracy,calibrateatsetpoint
TECHNICAL SUPPORT / SERVICE
ShouldyourI-5311-DSshakingincubatorrequireservice,callLabnetInternational’sService
Departmentat732-417-0700betweenthehoursof9:00amand5:00pmEST. Ifservice
necessitatesthereturnoftheunittoLabnet’sServiceDepartment,areturnauthorization(RA)
numbermustfirstbeobtainedandadecontaminationcertificatecompleted.Theunitmustbe
thoroughlydecontaminatedbeforeshipping. Theunitmustthenbesufficientlypackedfor
shipment.Anyshippingdamageonreturnedunitswillbethecustomer’sresponsibility.

ACCESSORIES
I-5322 Fullshelfw/brackets
I-5323 Halfshelf w/brackets
I-5330 Universalflaskclampplatform
I-5331 Flatmatplatform
I-5330-50 30x50mlflasksplatform
I-5330-125 20x125mlflasksplatform
I-5330-250 12x250mlflasksplatform
I-5330-500 8x500mlflasksplatform
I-5330-1000 6x1000mlflasksplatform
I-5330-2000 4x2000mlflasksplatform
Flaskclampsareavailablein50ml,125ml,150ml,500ml,1000ml,and2000ml.
Testtuberackclampsarealsoavailable.
Labnetiscontinuouslyaddingaccessories. CheckwithyourrepresentativeorcallLabnetdirectly
at732-417-0700orcheckourwebsite:www.labnetlink.comforacompletelistingofaccessories
andflaskclamps.