Labomed Lx 400 User manual

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Part No: 9126000-795
Issue 1.5
Printed on April,2019
Lx 400
Research Microscopy
User Manual
To ensure proper use of this instrument as well as to avoid injury while operating
Instrument, understanding this manual completely before use is highly recommended.

TABLE OF CONTENTS
1. INTRODUCTION 1
2. SAFETY INFORMATION 2-4
3. Lx 400 BINOCULAR 5
4. Lx 400 TRINOCULAR 6
5. UNPACKING YOUR MICROSCOPE 7
6. STANDARD COMPONENTS 8
7. OPTIONAL ACCESSORIES 9-11
8. INITIAL SETUP 12
9. ASSEMBLY 13-14
10. SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE 15
11. DETAILED OBSERVATION PROCEDURE 16-20
12. CLEANING & SERVICING 21-22
13. DISPOSAL 23
14. TROUBLESHOOTING GUIDE 24-25
15. SPECIFICATIONS 26
16. DIMENSIONS 27
Lx 400

The Lx 400 is a research microscope reflecting a modern design as well as the latest in optical and mechani-
cal advancements. Designed for professionals as well as clinicians, this microscope offers many features
and functions for a diverse of applications. Here are a few points highlighting the benefits of the Lx400:
-Extra clarity and contrast is provided through a 360°rotatable Binocular body inclined at 30°with IPD
adjustments.
-The pressure die cast stand consists of ball bearing, frictionless sideways focusing to avoid any loss in
motion.
-The sturdy new stylish design provides a high degree of comfort as well as stability.
-The high powered objectives are spring loaded to prevent accidental damage to specimen slides.
-The reverse angle quadruple nose piece has a comfortable ribbed grip for easy rotation that also safe
-guards the turret system against any damage. All positions are par-centered and par-ensuring the highest
level of accuracy.
-The ball bearing mechanical stage allows smooth travel over a 78 x 54mm area with spring loaded stage
clamps for retaining the specimen at the exact position desired. A 0.1mm verier scale provides accurate
location of specimen area.
-High power illumination is delivered through our well crafted Universal Power Supply and operates with
any 100V-240V AC input. The LED configuration is operational with an in-built rechargeable battery P/N
9135000-902, Which when plugged in, draws power from a Universal Power Supply operating at 100V-240
AC input. This ensures continuous operation even under fluctuation voltages.
-Our Halogen bulb (6V-20W) has an average life span of upto 2,000 hours. Our LED has an average life span
of upto 100,000 hours.
-The Lx 400 comes equipped with a removable N.A. 1.25 Abbe condenser with spheric lens for brighter
Illumination levels, and an diaphragm for better resolution and contrast control.
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Lx 400
1INTRODUCTION
9126000-795 Lx 400
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Fig. 1
SAFETY SYMBOLS
The following symbols are found on the microscope. For optimal use, it is recommended that users
Understand these symbols and always use the equipment as prescribed.
Symbol Explanation
This surface has a tendency to heat up and should not be touched unless
System has completely cooled down.
Before use, carefully read the instruction manual. Improper use could result in
Injury to the user and/or damage to the equipment.
Main switch is ON.
Warning against risk of electric shock.
Main switch is OFF
.
|
!
SAFETY INFORMATION
1. After the microscope has been used for observation of a speci
-men containing bacteria, clean all parts coming in contact with
the specimen to prevent infection.
•Be sure to remove the specimen before moving this product.
•In case the specimen is damaged by erroneous operation, it is
important to clean all surfaces that may have come in contact
with the specimen.
2. To avoid potential electrical hazards when replacing either halo
-gen bulb or LED, turn the microscope main switch to the OFF posi
-tion and disconnect power cord from wall outlet in advance.
Whenever you replace your microscope bulb, allow lamp socket
and bulb to cool before touching (Fig.1)
Applicable bulb/LED replacement: 6V20W Halogen bulb P/N CX-013; 6V30W Halogen bulb P/N EL-455 or
LED P/N 9135000-901
3. Install microscope on a sturdy, level table or bench and avoid any restriction of air vents in the base of
the unit.
Do not place microscope on a flexible surface, as this could result in blocking the air vents and cause
overheating.
4. Always use the power cord provided by LABOMED. If the proper power cord is not used, product safety
performance cannot be warranted.
5. When installing the microscope, route the power cord away from the microscope frame. Should the
power cord come in contact with the microscopes base, the power cord could melt due to overexposure
to heat.
6. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly
connected. If the unit is not grounded, LABOMED can not warrant electrical safety.
7. Never allow metallic objects to penetrate the air vents of the microscope frame as this could result in
user injury and damage to the microscope.
8. After operation of microscope, be sure to disconnect power cord from connector socket of the microscope
or from the wall power outlet.
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Fig. 2
Fig. 3
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Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may not be
warranted. In addition, the equipment may also suffer damage. Always use the equipment as outlined in
this instruction manual.
1. A microscope is a precision instrument with delicate glass compo-
nents. Please handle with care.
2. Do not use the microscope where it is subjected to direct sunlight,
high temperature, humidity, dust and vibrations. ( For the operat
-ing conditions, see chapter 14. “SPECIFICATIONS”)
3. The microscope is ventilated by natural convention. Be sure to
leave enough space (10cm or more) around body when installing
the unit.
4. Arm handle is provided for carrying the microscope.
To prevent damage, do not hold the microscope by the stage or
observation tube.
Be sure to remove the specimen from the stage clip while
transporting unit to avoid damage of the specimen slide.
1. Clean all glass components by wiping gently with cleaning cloth provided. To remove fingerprints or oil
smudges, wipe with cleaning cloth slightly moistened with a mixture of petroleum (85%) and isopropa
-nol (15%).
Since solvents such as petroleum and isopropanol are highly flammable, they must be handled carefully.
Be sure to keep these chemicals away from open flames or potential Sources of electrical sparks - For
Example, electrical equipment that is being switched “ON” or “OFF”. Also remember to always use these
chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass compo
-nents. To clean non-glass components, use a lint-free, soft cloth slighty moistened with a diluted neutral
detergent.
3. Do not disassemble any part of the microscope as this could result in malfunction or mitigated perfor
-mance.
4. When not using the microscope, ensure that the frame is fully
cooled before storing the unit in a dry locker or covering with a
dust cover (provided).
5. To clean the condenser/phase turret, fully loosen the securing
thumb screw (1) and remove the condenser then, wipe the front
lens of the condenser with optical cleaning solution (mixture
suggested above) and lens tissue.
The condenser can be re-attached by replacing the condenser in
its seat, tightening securing thumb screw, and raising condens-
er bracket to desired position.
6. Be sure to observe your local rules/regulation for product
disposal.
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Lx 400
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1GETTING STARTED
MAINTENANCE AND STORAGE
9126000-795 Lx 400
Issue 1.5
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Zig-zag motion (X) Spiral motion ( )
Your microscope had been engineered for a long and safe operational life with the least amount of main-
tenance required. In general, routine maintenance is limited to keeping the microscope working parts
lubricated and optics clean. Always cover the microscope with the provided dust cover when not in use.
Optical Cleaning
1. The objective have been adjusted for a tight fit to prevent any damage during transportation. To remove
an objective, rotate it counterclockwise while gripping it with a rubber sheet, etc. to avoid any slip page.
2. To clean the lens surfaces, remove dust using a soft brush or gauze (compressed air dust cans are
ideal). For removing finger marks or grease, soft cotton cloth or lens tissue lightly moistened
with cleaning solution ( 85% petroleum ether and 15% isopeopanol) should be used. For cleaning the
optics, use Meathanol. Observe sufficient caution in handling Methanol. Place the Objective and/ or
eyepieces on a dust free surfaces (e.g. aluminum foil). All other optical components to be cleaned should
be as accessible as possible.
3. Blow all loose dust particles away with a dust blower.
4. Remove all water-soluble dirt with distilled water. If this is unsuccessful repeat using a solution of
diluted hand soap liquid. Remove any remaining residue with a dry cotton swab.
5. To remove oil, use a solution of diluted hand-soap liquid initially. If this does not produce a satisfac-
tory result, repeat the cleaning using a solvent (Optical Cleaning Solution 85% petroleum ether and
15% isopropanol).
6. Grease must always be removed using a solvent.
7. Cleaning is achieved by using a spiral motion from the center to the rim. Never wipe using zig-zag
movements as this will only spread the dirt. With larger optical surfaces (e.g. tube lenses) the spiral
motion starts initially at the rim before to the middle and is only then followed by a center to rim
cleaning motion. Normally several spiral wipes are recommended.
We recommend pure, volatile petroleum ether or Optical Cleaning Solution as explained in point 3 above.
Wipe using a spiral movement. Do not use a zig-zag motion!
Cleaning of painted surfaces
Avoid the use of any organic solvent (e.g. thinner, xylene, ether, alcohol etc.) for cleaning of painted
surfaces of the instrument. Painted surfaces can be cleaned with a very lightly moistened micro fiber
cloth. Loose dust and dirt can be removed using a brush of soft hair used exclusively for this purpose.
Lx 400
3CARE & MAINTENANCE
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X-Y movement control knob
Binocular viewing tube, 30° inclined
Observation tube
Clamping screw
Coarse and
fine focus knob
Mechanical
stage
On/Off intensity regulator
Specimen holder
Revolving nose piece
Objectives
Eyepieces
Abbe condenser
Filter holder
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Lx 400 BINOCULAR
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Lx 400
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9126000-795 Lx 400
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X-Y movement control knob
On/Off intensity regulator
Eyepieces
Revolving nose piece
Objectives
Specimen holder
Coarse and
fine focus knob
Mechanical
stage
Observation tube
clamping screw
Trinocular viewing tube
Trinocular Port
Abbe condenser
Filter holder
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Lx 400
Lx 400 TRINOCULAR
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9126000-795 Lx 400
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Microscope Arm
Observation head
Eyepieces
Power Cord
UNPACKING YOUR MICROSCOPE
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Lx 400
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Daylight (blue) filter
Power Cord
Allen Wrench 3mm
Paired Eyepieces
6V 20W Halogen bulb
Monocular/ Binocular/ Trinocular observation tube
Allen screw
Microscope Arm
Stage
Objectives
Eyepieces
(Fixed at 10x)
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STANDARD COMPONENTS
After removing your microscope from its packaging, make sure that all of the following contents are present.
“Please note that the contents of your microscope may vary as the optional configuration, contrasting
method or viewing body opted for may not be of the standard configuration highlighted here”.
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Lx 400
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SYSTEM DIAGRAM OF OPTIONAL ACCESSORIES
Fig. 4
1. Mount the Video adapter 1/2” on Trinocular observation head.
2. Mount Camera Module System on Video adapter.
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Lx 400
OPTIONAL ACCESSORIES
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INSTALLATION AND OPERATION OF OPTIONAL ACCESSORIES
CAMERA MODULE SYSTEM
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ABBE CONDENSER 0.6
ABBE CONDENSER 1.25 POLARIZING KIT
WF 16xWF 10x WF 20x
TRINOCULAR HEADBINOCULAR HEAD
RP 4x RP 10x RP 40x (SL)
RP 20x RP 50x (SL, OIL) RP 100x (SL, OIL) RP PH 10x RP PH 20x RP PH 40x (SL) RP PH 100x (SL, OIL)
RP FL 4x RP FL 10x RP FL 40x (SL)
RP FL 20x RP FL 50x (SL, OIL) RP FL 100x (SL, OIL) RP 100x (OIL, IRIS)
HALOGEN BULB LED MOUNT RECHARGEABLE BATTERY
BLUE FILTER GREEN FILTER
ATLAS CMOS CAMERA
C-MOUNT VEGA
TOUCH SCREEN
VEGA CMOS CAMERA
PHASE TURRET
C-MOUNT TOUCH SCREENC-MOUNT ATLAS

Fig. 5
Fig. 6
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2
34
Fig. 8
AB
Fig. 7
1
2
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10X eyepieces are provided. To replace:
1. Pull out the 10x eyepieces out from the observation heads
Ocular tube.
2. Insert desired eyepieces in empty ocular tube.
1. Remove the condenser from its position by loosening the thumb
screw (1) on right side of the condenser bracket as shown in
figure 6.
2. Place the phase annulus turret in position from where the
condenser has been removed.
3. Secure it with thumb screw (1). The phase turret can be adjusted
at any desired phase annulus (10x, 20x, 40x & 100x) by turning
the thumb wheel (2) to the desired position. There is also an ‘0’
setting on the thumb wheel for bright field application.
Note: Refer to the user manual provided with the Phase Contrast
fit for centering and alignment.
Procedure for Koehler Installation Kit (figure 7-11)
1. Lay the microscope on a clean surface toward right so that the
microscope base is exposed.
2. Using a screw driver, unscrew four screws (1 to 4) from (figure 7)
to open the base of the microscope.
3. Remove two screws (A) ans (B) from (figure 8) to open the
illumination mount and replace it with Koehler mount.
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Lx 400
OPTIONAL EYEPIECES
PHASE TURRET
INSTALLING THE KOEHLER ILLUMINATION KIT
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3
4
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Fig. 9
Fig. 10
Fig. 11
1
2
Lens A
Frosted
Lens A
Lens Spacer
Lens B
T Ring
Rotate anti clockwise
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9126000-795 Lx 400
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4. Open the illumination assembly as in (figure 9), by rotating
the T Ring. Replace the lens B with frosted lens provided with the
Koehler kit.
Secure the lens assembly back.
5. Close the base cover with screws.
6. Replace the blue filter with D lens. Refer figure 10.
7. Install the new Blue filter on the Koehler mount, figure 11.
CAUTION: The flat surface of the lenses will remain downwards for
Lens A and Lens B.

Fig. 12
Fig. 13
Fig.14
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Lx 400
Lx 400
Lx 400
Objectives are factory set. Objectives are par-centered and parfo-
calised during assembly.
All objectives have been secured for a tight fit to prevent them
from coming loose during transit. To remove an objective, rotate it
counterclockwise while holding it with a rubber grip to avoid any
slippage.
Install the observation head using the following procedure:
1. Using allen wrench 3mm (provided), loosen the Head Locking
Screw (1) and remove the dust cover cap provided in dovetail
cavity as well as on observation head dovetail.
2. Mount the Observation Head by engaging the dovetail provided
at the bottom of the head into the dovetail cavity provided in
the microscopes arm.
3. Tighten the Head Locking Screw (1) after positioning the Obser-
vation head as desired. See figure 13.
Insert the eyepieces into the ocular tube of Observation Head
using following procedure:
1. Remove the protective caps from the observation tube.
2. Insert 10x eyepieces into the ocular sleeve and tighten the
clamping screw (1) using allen wrench 1.2mm. See figure 14.
Lx 400
INITIAL SETUP
8
OBJECTIVES
1
OBSERVATION HEAD
2
EYEPIECES
3
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Each standard set can be assembled by simply attaching the bulb, filter and power cord.
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Lx 400
Fig. 15
Fig. 16
3
2
1
1
2
6
3
4
579
8
5
INSTALLING OR REPLACING THE LAMP BULB
PROCEDURE FOR LED REPLACEMENT
ASSEMBLY
9
1
2
Applicable LED replacement: LED P/N 9135000-901
1. Screws SS 658
2. Base Plate 9126100-301
3. Screws Heat Shield SS 714
4. Heat Shield 9135000-524
5. Star LED 9135000-901
6. LED Screws SS 658
7. Stand
8. Back Cover Plate 9126000-102
9. Electric Panel 9126000-103
10. Decorative Cap 8123000-202
Before attaching the lamp bulb, remove the parts that may drop
such as the filter and specimen from the microscope frame, and
place the microscope in its back so that the bottom plate is
exposed.
1. Pull the lock knob (1) on the bottom to opera lamp housing door
(fig.15).
2. Hold the halogen bulb (2) without taking it out of the polythyle-
ne bag so as not to taint the bulb with fingerprints and puch the
bulb into the pin holes on the socket (3). After attaching,remove
the polyethylene bag.
3. With the lock knob pulled out, close the lamp housing door,
then push the lock knob back to lock the cover.
Always use the designated bulb. Using a bulb other than those
specified by LABOMED may lead to a fore hazard or and low
illumination. Fingerprints or stains on the lamp bulb reduce its
life. If contamination occurs, wipe bulb surface with a cloth
slightly moistened with alcohol.
Figure 16
1. Pull out two decorative caps (shown as 9) from back cover
plate.
2. Using Cross head screw driver, unscrew two screws (shown as
9) to detach back cover plate from stand.
3. Lay down the microscope on back cover over a clean surface to
expose base plate.
4. Using Cross head screw driver, unscrew four screws (shown as
1) to open the bottom plate of the microscope.
5. Cut LED wire holding tie and Detach LED wire connector from
PCM mounted on back cover plate.
6. Detach Heat Shield from critical mount by opening 3 screws
(shown as 3) with cross head screw driver.
7. Detach LED from Heat Shield by loosening 2 screws (shown as
6).
8. Replace new LED on Heat Shield and connect wire connections
to PCB.
9. Reverse steps 6 to 1, to complete the process.
CAUTION: For Bulb Replacement During Use or Right After Use
The bulb, lamp socket and areas near these will extremely hot during and right after use. Set the main
switch to “O” (OFF), disconnect the power cord from the wall outlet, and allow the bulb ans lamp socket
to cool before replacing the bulb with a new bulb of the designated type. Cooling time may vary to users
Discretion.
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Lx 400
Fig. 17
Fig. 18
Fig. 19
3
4
5
1
1
2
2
1
23
4
5
3
6
PROCEDURE FOR BATTERY REPLACEMENT
MOUNTING THE DAYLIGHT (BLUE) FILTER
INSTALLING OR REPLACING THE FUSE
1. Screws for Battery cover bottom
SS 658
2. Battery cover 9135100-204
3. Battery 9135000-903
4. Cable clamp EL-488
5. Battery clamp X54383
6. Screws Battery clamp SS 658
Applicable LED Battery Replacement: LED P/N 9135000-903
Figure 17
1. Lay down the microscope om back cover a over clean surface
to expose base plate.
2. Using Cross head screw driver, unscrew four screws (shown
as 1) to open the battery cover from bottom plate.
3. Detach connector of connecting wires (shown as 4)
4. Remove two screws (shown as 6) provided on battery clamp
with Cross head screw driver.
5. Replace existing Battery with new battery from LABOMED
(Refer part no. below)
6. Reverse steps 5 to 1, to complete the process.
This filter modifies the color of observation light into a natural
(daylight color).
•Fit the filter (1) into the bottom of the condenser (2) until it
clicks into (Figure 18).
Before replacing the fuse, remove the parts that may drop such as
the filter and specimen from the microscope frame. Turn around
the microscope to its back so that the AC inlet is visible (Fig. 19).
1. Use a flat head screw driver to open the fuse holder (Shown a 1).
2. The fuse tray will come out with live fuse (Shown as 2) and
spare fuse (Shown as 3). Do not pull out the fuse tray with
force as it is locked and will not be out completely.
3. Replace the primary fuse (Shown as 2) with the spare fuse.
4. Engage the fuse tray back in.
Always use the designated Fuse. Using a fuse other than those
specified by LABOMED may lead to a fire hazard.
CAUTION: For Fuse replacement Set the main switch to “O”(OFF),
disconnect the power cord from the wall outlet.
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Flip the main switch to “ON”
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen in focus.
Bring the specimen in light path using X-Y
Motion of mechanical stage.
Adjust the aperture iris diaphragm.
Engage the objective to be used in the light
path and bring the specimen in focus.
Engage the required filters if not pre-installed.
Adjust the observation tube and eyepieces
Adjust the interpupillary distance.
Adjust the dioptric setting.
Adjust the brightness.
Observe Specimen.
Main switch
Fuse Holder
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Lx 400
10SUMMARY OF BRIGHT FIELD OBSERVATION PROCEDURE
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16
TURNING THE LAMP ON
PLACING SPECIMEN ON THE STAGE
Fig. 20
Fig. 21
Fig. 22
1
2
1
32
5
4
DETAILED OBSERVATION PROCEDURE
11 Lx 400
1
2
1. Flip the main switch to “I” (ON) as shown in figure 20.
2. Rotating the light intensity adjustment knob (Fig. 21) in the
direction of the arrow increases brightness and rotating
knob in the opposite direction decreases brightness. The int-
ensity bar next the knob indicates the direction of intensity
level.
1. Rotate the coarse adjustment knob (2) in anticlockwise
direction to fully lower the stage.
2. Open the bow-shaped lever (3) outward by pulling on lever
handle (1), place the specimen by sliding the specimen glass
plate (s) on the stage from the front toward the slide seat at
the rear.
3. After positioning your specimen slides, (2max) return the
bow-shaped lever (3) gently by slowly releasing control
knob (1).
4. Rotating the upper co-axial knob controlling the y-axis
movement (4) moves the specimen in the vertical direction.
Rotating the lower knob (%) moves the specimen in the X-
axis or horizontal direction.
Do not adjust the specimen holder directly by hand, for this
will damage the rotary mechanisms.
CAUTION: When the specimen holder reaches stop position,
the rotation force of the X/Y knobs become stiff. Stop rotat-
ing at this point.
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Fig. 23
Fig. 24
Fig. 25
WD
WD (mm)
4X
30.0 4.5 0.50 0.13
10X 40X 100X
Objective
Magnification
2
2
3
1
1
Slide glass
Cover glass
Lx 400
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3ADJUSTING THE FOCUS
Cover glass
This is the glass plate paced on the specimen. For optimum optical
performance, the cover glass thickness, which is the distance
from its surface to the specimen surface, should be
0.17mm.
Slide glass
This glass plate should ideally have a length of 76mm, width of
26mm ± 1mm and thickness between 0.9 and 1.4mm.
“Vernier Scales”
These scales allow for easy identification of the specimen’s posi-
tion (coordinates), making it easy to return to a particular region
of interest after scanning the slide. (Figure 24)
1. The horizontal coordinate can be read at position (1) on the
specimen holder.
2. The vertical coordinate can be read at the index line (2).
Focusing Procedure (Figure 25)
1. Rotate the coarse adjustment knob (1) clockwise so that the
objective (3) is as close as possible to the specimen (we
recommend starting with 10X).
2. While observing the specimen through the eyepieces, slowly
rotate the coarse adjustment knob (1) counterclockwise to lower
the stage.
3. When coarse focusing of the specimen is obtained (an image is
observed), rotate the fine adjustment knob (2) for fine
detail focusing.
Working Distance (WD)
The WD refers to the distance between each objective and the
specimen, when acute focus of the specimen is obtained.
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