Leica BIOSYSTEMS SM2010R User manual

Instructions for Use
Leica SM2010 R
Sliding Microtome
Instructions for Use
Leica SM2010 R, V 1.4 RevC – 09/2013, English
Order No.: 14 0508 80101 RevC
Always keep this manual with the instrument.
Read carefully before working with the instrument.

1
Leica SM2010 R
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D –69226 Nussloch
Germany
Phone: [phone]
Fax: [phone]
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and state-of-
the-art technology as we understand it following
thorough investigation in this field.
We are under no obligation to update the present
manual periodically and on an ongoing basis ac-
cording to the latest technical developments, nor
to provide our customers with additional copies,
updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for errone-
ous statements, drawings, technical illustrations
etc. contained in this manual. In particular, no
liability whatsoever is accepted for any financial
loss or consequential damage caused by or
related to compliance with statements or other
information in this manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present Instructions for Use are not
to be considered as warranted characteristics of
our products.
These are determined only by the contract
provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical spec-
ifications as well as manufacturing processes
without prior notice. Only in this way is it pos-
sible to continuously improve the technology and
manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – includ-
ing any electronic systems and media – requires
express prior permission in writing by Leica Bio-
systems Nussloch GmbH.
For the instrument serial number and year of
manufacture, please refer to the name plate at
the rear of the instrument.
© Leica Biosystems Nussloch GmbH
NOTE

2Instructions for Use V 1.4 RevC – 09/2013
Table of contents
1. Important Information............................................................................................................................. 3
2. Safety ......................................................................................................................................................... 4
2.1 Safety notes .................................................................................................................................................. 4
2.2 Warnings ....................................................................................................................................................... 4
3. Instrument Components and Specifications....................................................................................... 7
3.1 Overview - instrument components.......................................................................................................... 7
3.2 Technical data .............................................................................................................................................. 8
4. Instrument Setup.................................................................................................................................... 10
4.1 Standard delivery ....................................................................................................................................... 10
4.2 Site requirements....................................................................................................................................... 10
4.3 Unpacking.................................................................................................................................................... 11
4.4 Setup ............................................................................................................................................................ 11
4.5 Assembling the knife holder SN .............................................................................................................. 13
4.6 Assembling the blade holder SE.............................................................................................................. 16
5. Operation................................................................................................................................................. 21
5.1 Operating elements and their functions .............................................................................................................21
5.1.1 Section thickness setting.......................................................................................................................... 21
5.1.2 Coarse driving wheel................................................................................................................................. 21
5.1.3 Manual feed ................................................................................................................................................ 22
5.1.4 Automatic feed ........................................................................................................................................... 22
5.1.5 Directional fixture for specimen clamps ................................................................................................ 23
5.2 Clamping the specimen in the universal cassette clamp (UCC)......................................................... 24
5.3 Clamping a disposable blade ................................................................................................................... 25
5.4 Replacing the pressure plate ................................................................................................................... 26
5.5 Inserting the knife ...................................................................................................................................... 27
5.6 Sectioning.................................................................................................................................................... 28
5.7 Changing the specimen or interrupting sectioning .............................................................................. 29
6. Cleaning and maintenance .................................................................................................................. 30
6.1 Cleaning the instrument ............................................................................................................................ 30
7. Optional accessories ............................................................................................................................ 32
8. Troubleshooting ..................................................................................................................................... 39
8.1 Possible faults............................................................................................................................................. 39
8.2 Instrument malfunctions ........................................................................................................................... 39
9. Warranty and Service ........................................................................................................................... 40
10. Notes ........................................................................................................................................................ 41
11. Decontamination Certificate (Master)............................................................................................... 42

3
Leica SM2010 R
Symbols in the text and their meanings
1. Important Information
(5)
Intended use of instrument
The Leica SM2010 R is a manually operated
sliding microtome for creating thin sections of
specimens of varying hardness for use in routine
and research laboratories in the fields of biology,
medicine and industry.
It is designed for sectioning soft paraffin speci-
mens as well as harder specimens, as long as
they are suitable for being cut manually.
Any other use of the instrument is considered
improper!
Instrument type
All information provided in these Instructions for
Use applies only to the instrument type indicated
on the title page. A nameplate indicating the in-
strument serial number is attached to the rear side
of the instrument. The figure below is provided as
an example only and shows a valid nameplate for
this instrument.
Qualification of personnel
• TheLeicaSM2010Rmaybeoperatedby
trained laboratory personnel only. Fig. 1
Warnings
appear in a gray box and are marked by
a warning triangle .
Notes,
i.e. important user information appears
in a gray box and is marked by an infor-
mation symbol .
Numbers in parentheses refer to item
numbers in illustrations.
Manufacturer
This product fulfills the requirements
of the Council's Directive 98/79/EC
concerning in vitro diagnostics (IVD)
medical devices.
In vitro diagnostics (IVD) medical device
Observe the Instructions for Use
Order No.
Serial number
Date of Manufacture
• Alllaboratorypersonneldesignatedtooperate
the Leica instrument must read these Instruc-
tions for Use carefully and must be familiar
with all technical features of the instrument
before attempting to operate it.

4Instructions for Use V 1.4 RevC – 09/2013
2. Safety
2.1 Safety notes
These Instructions for Use include important
information related to the operating safety and
maintenance of the instrument.
The instruction manual is an important part of
the product, which must be read carefully prior
to Instrument Setup and use and must always be
kept near the instrument.
This instrument has been built and tested in ac-
cordance with the Machinery Directive 2006/42/
EC, and the safety regulations governing labora-
tory apparatus.
To maintain this condition and ensure safe opera-
tion, the user must observe all notes and warnings
contained in these Instructions for Use.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis for
accident prevention. Primarily responsible for accident-free operation is above all the owner
of the instrument and, in addition, the designated personnel who operates, services or cleans
the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Be sure to comply with the safety instructions and warnings provided in this chapter.
Be sure to read these instructions, even if you are already familiar with the operation
and use of other Leica products.
These Instructions for Use must be ap-
propriately supplemented as required
by the existing regulations on accident
prevention and environmental safety in
the operator‘s country.
The current EC Declarations of Confor-
mity can be found on the Internet:
http://www.LeicaBiosystems.com
The protective devices on both instrument and accessories may neither be removed nor
modified. Only service personnel qualified by Leica may repair the instrument and ac-
cess the instrument’s internal components.

5
Leica SM2010 R
2. Safety
Warnings - Safety instructions / warning labels attached to the instrument
Warnings - Transport and installation
Warnings - Working at the instrument
Warnings - Cleaning and maintenance
• Safety notes on the instrument itself, which are marked with a warning triangle, indicate that
the correct operating instructions (as defined in these Instructions for Use) must be followed
when operating or replacing the item marked. Failure to adhere to these instructions may re-
sult in an accident, personal injury, damage to the instrument or accessory equipment.
• Once unpacked, the instrument may be transported only in an upright position.
• Before transporting the instrument, the knife sledge must be locked with the locking knob
(23, Fig. 2)!
• Do not transport the instrument by holding it by the knife sledge, coarse driving wheel or the
knob for setting the section thickness.
• Be very careful when handling microtome knives or blades. The cutting edge is extremely
sharp and can cause serious injuries!
• Always wear work safety shoes and safety gloves!
• Never place a knife anywhere with the cutting edge facing upwards and never try to catch a
falling knife! Always put the knives back into the knife case when not in use!
• Always clamp the specimen block BEFORE clamping the knife.
• Lock the knife sledge and cover the knife edge with the knife guard prior to any manipulation
of knife/blade or specimen, prior to changing the specimen block and during all work breaks.
• Always wear protective glasses and a mask when sectioning brittle specimens!
• Risk of splintering!
• Before each cleaning, remove the knife or disposable blade!
• Do not use solvents that contain acetone or xylene!
• Ensure that liquids do not enter the interior of the instrument during cleaning!
• When using cleaners, please comply with the safety instructions of the manufacturer and the
laboratory safety regulations!

6Instructions for Use V 1.4 RevC – 09/2013
2. Safety
2.3 Integrated safety devices The instrument is equipped with the
following safety devices:
• Knifeguard(14) on the blade/knife
holder.
• Knifesledgelockingknob(23) for
the knife sledge.
14
24
23
Knife guard on the blade holder
The blade holder is equipped with a tightly mounted knife
guard (14). This makes it possible to cover completely the
cutting edge of the blade (Fig. 3).
Knife sledge locking knob
The knife sledge is locked in place us-
ing the locking knob (23) that engages
in the notch points on the bar (24), thus
holding the knife sledge securely. The
bar has 11 notch points, with a dis-
tance of 10 mm between each.
14
14
11
Knife guard on the knife holder
Knife guard on the blade holder
Knife sledge
locked
Knife sledge
is movable
Prior to any manipulation
of the knife or specimen, or
each change of specimen,
and during breaks, always
cover the cutting edge of
the blade/knife with the
knife guard (14)!
Caution!
When the knife guard is
pushed over the blade, do
not reach into the blade
from below!
Prior to changing the speci-
men or knife and before
transporting the instrument,
lock the knife sledge (11)
using the locking knob (23).
Fig. 3
Fig. 2

7
Leica SM2010 R
3. Instrument Components and Specifications
Specimen cylinder
Removable
section waste tray
3.1 Overview - instrument components
Knife sledge
Ergo handle for
moving the knife
sledge
Blade holder SE
Coarse driving
wheel
Adjusting knob of
automatic feed
Knife guard on
the blade holder
Fig. 4
Magnetic
knife sledge
immobilizer
Setscrew for
orientation in cutting
direction
Adjustable
instrument feet
HN40
tensioning clamp
Lever for
clamping the
blade
Scaled adjusting knob
for setting the section
thickness
Lever for manual feed
Clamping lever for
lateral displacement
Clamping lever for
specimen orientation
Knife sledge
lock
Clearance angle
scale
Knife holder SN
Setscrew for
orientation
perpendicular to
cutting direction
Universal
cassettes
clamp
Quick clamping
system for holding
the specimen
clamps
Knife guard on
the knife holder

8Instructions for Use V 1.4 RevC – 09/2013
3. Instrument components and specifications
3.2 Technical data
General
Approvals: The instrument-specific marks are located on the
name plate.
Operating temperature range: 10 °C to +40 °C
Relative humidity: max. 80% non-condensing
Operating temperature range during storage: + 5 °C to +55 °C
Storage humidity: < 80 %
Microtome
Section thickness range: 0.5 - 60.0 µm
Section thickness settings: from 0.5 - 5.0 µm in 0.5 µm increments
from 5.0 - 10.0 µm in 1.0 µm increments
from 10.0 - 20.0 µm in 2.0 µm increments
from 20.0 - 60.0 µm in 5.0 µm increments
Automatic specimen feed: from 0 to 30 µm
Total specimen stroke: approx. 50 mm
Clearance angle adjustment: –3° to 10°
Maximum specimen size: 50 x 60 x 40 mm
Specimen orientation
in cutting direction: ± 8°
perpendicular to cutting direction: ± 8°
Declination: 0° - 45° in cutting direction
Dimensions and weight
Width (with coarse driving wheel
and Ergo handle): 390 mm
Width (base plate): 256 mm
Depth: 430 mm
Height (total): 343 mm (with blade holder)
Working height (knife blade): 255 mm (measured from the table)
Weight (without accessories): Approx. 20 kg

9
Leica SM2010 R
3. Instrument Components and Specifications
3.3 Instrument specifications
• TheLeicaSM2010Risamanuallyoperatedslidingmicrotome,designed
as a low-maintenance tabletop instrument with roller-guided knife
sledges and automatic section thickness feed.
• Stable,torsion-freebasicdesignwithmicrometerfeedsysteminaclosed
housing, protected from ingress of paraffin waste.
• Theverticalcrossrollerbearingshaveacoverwhichprovidesreliable
protection from ingress of section waste.
• Theinstrumenthasanergonomicallyoptimizedspecimenheadposition;
the smooth-running knife sledge can be securely locked in 10 mm incre-
ments.
• Precise8°XYorientationwithdefinedzeroposition.
• IndividuallyadjustableErgohandleforeasilymovingthesledge.
• Thesectioningwindowcanbeadjustedtothespecimensize.
• Thecuttingthicknesscanbeadjustedwithintherangeof0.5µmto60µm;
the automatic feed functions in the range of 0.5 µm to 30 µm.
• Manualfeedbypullingorpushingthefeedlever.
• Dependingontheversion,theinstrumentiseitherfittedwithablade
holder SE for disposable blades or a knife holder SN for conventional
knives. The blade holder and knife holder each have an integrated knife
guard. In the knife holder SN a blade rail can also be inserted for holding
disposable blades.
• Theknifeordisposablebladeholderdoesnotneedtoberemovedfor
the clearance angle adjustment.
• Thesmooth-turning,coarsedrivingwheelcanbechosenwithclockwise
or counterclockwise rotation.
• Differentspecimenclampscanbeinsertedinthequickclampingsystem.
• Theinstrumenthasanantistaticwastetrayforholdingalargevolume.

10 Instructions for Use V 1.4 RevC – 09/2013
4. Instrument Setup
4.2 Site requirements
• Stable,vibration-freelaboratorytablewithhorizontal,flattabletop,as
far as possible vibration-free ground.
• Nootherinstrumentsnearbywhichmightcausevibrations.
• Roomtemperatureconsistentlybetween+10°Cand+40°C.
• Freeaccesstocoarsedrivingwheelandknifesledge.
• Theinstrumentissuitableforoperationinenclosedroomsonly.
4.1 Standard delivery
The Leica SM2010 R standard delivery includes:
1 Leica SM2010 R basic instrument.............................................. 14 0508 42258
1 section waste tray........................................................................ 14 0508 42328
1 tool set consisting of.................................................................... 14 0508 42983
1 Allen key size 6.............................................................................. 14 0194 43634
1 Allen key with handle, size 4....................................................... 14 0194 04782
1 Allen key T 25 ................................................................................ 14 0194 45250
1 open-end wrench, size 10........................................................... 14 0330 04158
1 spare magnet ................................................................................ 14 0508 44762
1 dust protection cover................................................................... 14 0212 18961
1 pair of safety gloves, size S ........................................................ 14 0340 40859
1 Instructions for Use...................................................................... 14 0508 80101
1 language CD.................................................................................. 14 0500 80200
The accessories ordered are included in a separate box.
Carefully check the delivery against the packing list and the deliv-
ery note. Should you find any discrepancies, please contact your
Leica sales office without delay.

11
Leica SM2010 R
4.3 Unpacking
• Tolifttheinstrumentfromthebox,holditon
the left and right of the housing (Fig. 5), lift it
out of the foam cushion of the package and
place it on a stable lab table.
• Removealladhesivetapeusedastransport
anchors.
4.4 Setup
Loosening the transport locking screw of the
knife sledge
23
11
12
Fig. 6
4. Instrument Setup
First check the shipment for external damages upon arrival.
If it is evident that the shipment was damaged during transport,
please make a claim to the carrier immediately.
• Openthepackaging.
• Removeallfoammaterial.
• Takeoutallaccessoriesandtheinstructionmanual.
Do not transport the instrument by hold-
ing it by moving parts, on the knife
sledge, coarse driving wheel or the
knob for setting the section thickness.
Fig. 5
The locking knob (23) secures the knife
sledge during transport.
In daily operation, it is also used to lock
the knife sledge in place.

12 Instructions for Use V 1.4 RevC – 09/2013
4. Instrument Setup
25
26
Horizontal alignment
For safe and accurate work, it is important that
all instrument feet are in uniform contact with the
installation surfaces.
The microtome is horizontally aligned at the fac-
tory. If a completely level or horizontal surface is
not available at the installation site, the instrument
must be realigned.
To do so, the two instrument feet (25) on the right
side of the instrument are height-adjustable.
• Foralignment,loosenthelocknuts(26) using
a size 10 open-end wrench.
• Adjusttheinstrumentfeet(25) until the micro-
tome is in a stable position at the installation
location according to requirements.
• Retightenthelocknuts.
Setting the Ergo handle
The Ergo handle (12), which is used to move the
knife sledge, can be set individually to an ergo-
nomic gripping position.
• Todothis,loosenthefastening screw (10)
using a size 6 Allen key (28).
• Turnthehandle(12) to the desired position
and retighten the screw (10).
12
28
10
Fig. 7
Fig. 8

13
Leica SM2010 R
4.5 Assembling the knife holder SN
• Locktheknifesledge(11) in place using the
locking knob (23) so that it cannot be moved.
• Theintermediateplate(60) intended for the
knife holder SN, is mounted on the mounting
table (62) of the knife sledge (11).
Installing the intermediate plate
• AsshowninFig.9,placetheintermediateplate
on the mounting table. Make sure that the two
index markings (65) are at the top and point
towards the back.
• Insertthefivecountersunkscrews(61.1–61.5)
into the bores and tighten them crosswise (in
the sequence shown in Fig. 10) using an Allen
key T25 (63).
23
23
60
62
65
64.1
63
61.3
61.5
61.1
61.2
61.4
64.2
11
4. Instrument Setup
Fig. 9
Important!
The mounting table must be absolutely
clean and dry. There must not be any
foreign particles on it. Otherwise, sec-
tions could become contaminated.
Fig. 10

14 Instructions for Use V 1.4 RevC – 09/2013
Fastening the knife holder
• Presstheclampinglever (66) of
the knife holder (67) downwards
and screw the bottom of the
thread (66.1) into one of the two
bores (64.1) or (64.2, Fig. 11) of the
intermediate plate (60).
66
66.1
64.1
64.2 60
67
66
Assembling the knife holder SN (continued)
4. Instrument Setup
• Continue turning theclamping
lever (66) in a clockwise direc-
tion until the knife holder is firmly
screwed on (Fig. 12).
67
60
The clamping lever (66)
has a plastic handle that
can be turned into what-
ever position is best. To
do so, pull the handle up-
wards and turn it into the
desired position (Fig. 12).
It will then lock automati-
cally when released.
The knife holder SN (67)
has two bores (64) and
thus two different mount-
ing positions for different
sectioning requirements.
Fig. 11
Fig. 12

15
Leica SM2010 R
Fig. 13
Setting the clearance angle (Fig. 13)
• Unscrewtheknurledheadscrew(69).
• Settherequiredanglewiththeadjustinglever
(70) using the scale for the clearance angle
(71). The upper edge (73, red arrow in Fig. 13)
of the lever (70) has to align with the index
mark of the selected degree value.
• Tofixthesettinginplace,retightentheknurled
screw (69).
The clearance angle can also be adjusted if a
knife is clamped.
• Todothis,slightlyunscrewthetwoclamping
screws for the knife (72) and, if necessary, also
the knurled head screw (69).
• Setthedesiredangleasdescribedabove.
• Tofixthesettinginplace,retightentheknurled
screw (69).
Enlarged detail:
Scale for
setting the
clearance
angle.
72
69
70
73
4. Instrument Setup
Inclination (declination)
of the knife holder SN
• Loosentheclampinglever(66).
• Settherequireddeclination(inclinationofthe
knife holder from the cutting direction) on the
scale (55.1) at the back of the knife holder (67).
• Ontheintermediateplate(60) there is an index
mark (68.2) which serves as a point of refer-
ence for the scale division when adjusting the
declination.
• Forfixation,tightentheclampinglever(66)
firmly in the required position.
66
67
60
68.1
68.2
Fig. 14
Assembling the knife holder SN (continued)
71
70

16 Instructions for Use V 1.4 RevC – 09/2013
4.6 Assembling the blade holder SE
4. Instrument Setup
Mounting the grooved plate
• Locktheknifesledge(11) in place using the
locking knob (23) so that it cannot be moved.
• Thegroovedplate(50), which is intended for
the blade holder SE, is mounted on the mount-
ing table (62) of the knife sledge (11).
23
50
62
65
11
• AsshowninFig. 15, place the grooved plate on
the mounting table. Make sure that the groove
(54) for inserting the blade holder has the
rounded opening (56) for the T-piece (Fig. 16)
pointing forward.
• Insertthesixcountersunkscrews(61.1–61.6)
into the bores and tighten them crosswise (in
the sequence shown in Fig. 16) using an Allen
key T 25 (63).
23
63
61.3
61.5
61.1
61.2 61.4
61.6
56
The blade holder SE is designed for conventional
disposable blades from all common manufactur-
ers.
It is available in two models: one for low-profile
blades and one for high-profile blades.
The blade holder SE has a lateral movement, so
that the entire width of the blade can be used.
54
54
Important!
The mounting table must be absolutely
clean and dry. There must not be any
foreign particles on it. Otherwise, sec-
tions could become contaminated.
Fig. 15
Fig. 16

17
Leica SM2010 R
4. Instrument Setup
Inserting the blade holder SE
• Pushthebladeholderfullytothebacksuch
that the T-piece can be inserted in the round
insertion opening (56).
• Thenturnthebladeholder(9) approx. 90 °
(Fig. 18), insert the stopper (18) that closes the
insertion opening (56), and tighten it using the
Allen key T25 (63).
• Toclampthebladeholderon the grooved
plate, tighten the screw (52) on the back of the
blade holder base (17) using a size 6 Allen key
(Fig. 19).
Fig. 17
Fig. 18
18
54
• Mountthebladeholder(9) on the grooved
plate (50) such that the T-piece (53) on the
underside is inserted in the groove (54).
56
9
52
63
The stopper (18)
serves for clos-
ing the insertion
opening (56) for
the T-piece in the
grooved plate, in
order that no sec-
tion waste can ac-
cumulate there.
53
Assembling the blade holder SE (continued)
1.
2.
3.

18 Instructions for Use V 1.4 RevC – 09/2013
4. Instrument Setup
Inclination (declination)
of the blade holder SE
• UnscrewtheAllenscrew(52) at the back of the
blade-holder base using an Allen key SW 6.
• Turnthebladeholdertotherequiredposition.
• Settherequireddeclination(inclinationofthe
blade holder to the cutting direction) on the
scale (55.1) at the back of the blade holder (67).
• Onthegroovedplate(60) there is an index
mark (55.2) which serves as a point of refer-
ence for the scale division when adjusting the
declination.
• Forfixation,tightenthescrew(52) firmly in the
required position.
Fig. 19
52
55.2
55.1
Assembling the blade holder SE (continued)
19
27
27.1
Lateral movement
The lateral displacement feature enables the en-
tire cutting length of the blade to be used without
having to readjust the blade holder.
• Tomovethebladerail,turntheclampinglever
of the lateral displacement mechanism (19) on
the segment arc to the right into the "open"
position.
• Nowthebladerunner(20) can be moved later-
ally. To clamp, turn the lever (19) back to the
left.
The two triangular marks (27) indicate the area
in which the blade rail can be moved laterally,
relative to the circular mark (27.1).
Fig. 20