Lumencor SPECTRA III (L) Light Engine User manual

SPECTRA III (L) Light
Engine® Instruction Manual
Lumencor, Inc.!!!Document Number 57-10017!!!www.lumencor.com!

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Regulatory Models
Lumencor utilizes regulatory model names for all certified and CE marked products. The regulatory model
names are traceable to all regulatory documentation, third party reports and certifications.
“Regulatory Model: Spectra III L” is used as a representative model for all certified and CE marked
Spectra III Laser Products.
Emissions
This equipment has been tested and found to comply with the limits of EMC directive 2014/30/EU and FCC
part 15 (CISPR 11:+A1:2016). These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environment. This equipment generates, uses,
and can radiate radio frequency energy and, if not installed and used in accordance with the instruction
manual, may cause harmful interference to radio communications.
Safety Certifications
TUV SUD America, CB Certification (IEC 61010-1:2010)
TUV SUD America, NRTLus Certification (UL 61010-1:2012-05)
TUV SUD America, cNRTL Certification (CAN/CSA-C22.2 No. 61010-1:2012)
TUV SUD America, EN Certification (EN 61010-1:2010)
Underwriters Laboratories (UL), CB Certification (IEC/EN 60825-1:2007 & 2014 Safety of laser products)
CE Marking
Low Voltage Directive (2014/35/EU)
EMC Directive (2014/30/EU)
RoHS Directive (2011/65/EU)
REACH Regulation (EC) No. (1907/2006/EC)
EU Declarations of Conformity can be found at http://lumencor.com/company/regulatory-compliance/
Lumencor, Inc.
14940 NW Greenbrier Parkway
Beaverton, OR 97006
T 503.213.4269
www.lumencor.com
Document Number 57-10017
Revision B
031319
SPECTRA III (L) Light Engine® Instruction Manual
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Table of Contents
1. Introduction
2. Precautions and Warnings
3. Installation and Operating Instructions
4. Light Output Characteristics
5. Operational Specifications
6. Routine Maintenance and Trouble Shooting
7. Customer Support
8. Warranty
SPECTRA III (L) Light Engine® Instruction Manual
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1. Introduction
The SPECTRA III (L) light engine consists of up to 8 individually addressable solid-state light sources with
integrated electronic control systems. The constituent light sources may be LEDs, luminescent light pipes or
lasers. The outputs of the constituent light sources are refined by bandpass filters and merged into a
common optical train directed to the light output port on the front panel. The light output port has a built-in
adapter for connection to an SMA-terminated optical fiber or a liquid light guide (LLG). An onboard
photodiode continuously monitors the light output and generates a reference signal that is applied to the
constituent sources in a feedback loop to maintain constant light output over time. The light sources within
the SPECTRA III (L) light engine® are controlled by an on-board microprocessor operating Lumencor
firmware accessed via one of two serial interfaces, USB/RS-232 or TCP. The user can enable or disable
each source independently by serial commands as well as change the intensity of each source
independently. The SPECTRA III (L) light engine can either be controlled by third-party image acquisition
software or by a GUI resident on the on-board microprocessor. Alternatively, the light sources may be turned
on and off by TTL inputs from a trigger device such as a camera or a real-time controller. The only manual
control is the master power switch on the front panel. Optimal internal operating temperature is maintained
by negative pressure air cooling, with the air intake at the front of the light engine and the exhaust fan at the
the rear.
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2. Precautions and Warnings {Précautions et mises en garde}
A few simple practices will ensure trouble-free operation for the life of the light engine.
Les quelques règles simples suivantes permettront d’assurer un fonctionnement fiable pendant toute la
durée de service de la source lumineuse.
Safety Instructions:
Please read and follow all safety instructions provided BEFORE using the SPECTRA III (L) light engine®.
Failure to comply with the safety instructions may result in fire, electrical shock, or personal injury and may
damage or impair protection provided by equipment. Please save all safety instructions.
Instructions de sécurité:
Veiller à lire et à respecter toutes les instructions de sécurité fournies AVANT d’utiliser le SPECTRA III (L) light
engine®afin d’écarter les risques d’incendie, de décharge électrique, de blessure corporelle et de possibles
dommages ou défaillance de la protection offerte par l’appareil. Conserver toutes les instructions de sécurité.
Safety Definitions {Définitions relatives à la sécurité}:
Warning: Statements identify conditions or practices that could result in personal injury.
Avertissement:déclarations qui identifient des situations ou des pratiques susceptibles
d’entraîner des blessures corporelles.
Caution: Statements identify conditions or practices that could result in damage to your equipment.
Attention:déclarations qui identifient des situations ou des pratiques susceptibles d’endommager le
matériel.
Safety Items {Mesures de sécurité}:
Warning: ONLY use the power supply provided by Lumencor. The Lumencor-supplied 24 VDC, 9.2 A
external power supply is required for use with the SPECTRA III (L) light engine. The equipment is required to
be supplied by a properly approved/certified DC power source meeting the minimum electrical ratings of the
product. The DC power supply must have the AC power cord connected to a receptacle with a protective
safety (earth) ground terminal.
Avertissement: utiliser uniquement l'alimentation fournie par Lumencor. L'alimentation externe 24
VDC, 9,2 fournie par Lumencor est nécessaire pour une utilisation avec le moteur léger SPECTRA III (L).
L'équipement doit obligatoirement être alimenté par une source d'alimentation CC dûment approuvée /
certifiée respectant les caractéristiques électriques minimales du produit. Le cordon d'alimentation en
courant alternatif doit être connecté à une prise avec une borne de terre de protection.
SPECTRA III (L) Light Engine® Instruction Manual
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Warning: DO NOT look into the output of the light engine. The brightness of this light source is higher
than most commercial lighting fixtures and is intended to couple directly into a microscope or other
bioanalytjcal instrument.
Avertissement: NE PAS regarde directement la sortie de la source lumineuse. L’intensité lumineuse
de cette source est supérieure à celle de la majorité des appareils d’éclairage disponibles dans le commerce
et est conçue pour un raccordement direct à un microscope ou autre appareil de bioanalyse.!
Warning: DO NOT turn on the light without!the output end of the light guide safely!directed!into an
enclosed optical path. "DO NOT point the light output directly onto any flammable or burn-susceptible
material."This includes all animal or vegetable tissues, plastics, fabrics, paper and liquids.!
Avertissement: NE PAS allumer la lumière sans l'extrémité de sortie du guide de lumière dirigée en
toute sécurité dans un chemin optique fermé. NE PAS pointer la sortie de lumière directement sur un
matériau susceptible d'être inflammable ou susceptible de brûler. Cela comprend tous les tissus, les plastiques,
les tissus, le papier et les liquides animaux ou végétaux.
RISK GROUP 3
Warning: Possibly hazardous optical radiation emitted from this product. Do not look at operating lamp. Eye
injury may result.
Warning: Infrared (IR) emitted from this product. Do not look at operating lamp.
Warning: UV emitted from this product. Avoid eye and skin exposure to unshielded product.
GROUPE DE RISQUE 3
Avertissement: Infrarouge (IR) émise par ce produit. Ne regardez pas la lampe d'exploitation.
Avertissement: Rayonnement optique Peut-être dangereux émis par ce produit . Ne regardez pas la lampe
d'exploitation. Une blessure oculaire peut entraîner.
Avertissement: UV émis par ce produit . Évitez les yeux et la peau exposition au produit non blindé.
SPECTRA III (L) Light Engine® Instruction Manual
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Caution: Use of controls or adjustments or performance of procedures other than this specified herein
may result in hazardous radiation exposure.!
Attention: L'utilisation de commandes ou de réglages ou l'exécution de procédures autres que celles
spécifiées dans le présent document peuvent entraîner une exposition à des radiations dangereuses.
Class 4 Laser Warning
Warning: This product contains Class 4 laser
sources. Avoid eye and skin exposure to direct
or scattered visible and invisible laser radiation.
Avertissement: Ce produit contient des
sources laser de classe 4. Évitez l'exposition des
yeux et de la peau au rayonnement laser visible
ou dispersé visible et invisible.
Laser Aperture Warning
OR
Warning: Avoid exposure - laser radiation is emitted from this aperture. Do not turn on light engine without
first connecting a light guide to the output aperture. "The distal end of this light guide must be coupled into
an enclosed optical path prior to operation. Each operational control and laser aperture that can be
separated by 2 m or more from a radiation warning device shall itself be provided with a radiation warning
device. Do not exceed 2 m without providing a radiation warning device in accordance with EN
60825-1:2007 & 2014.
Laser Sources
Color
Center Wavelength (nm)
Power
Beam Divergence
See label located on
product.
300 - 1150
See label located on
product.
NA: 0.39 = 23 degrees
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Avertissement: Évitez l'exposition - le rayonnement laser est émis à partir de cette ouverture. L'ouverture
de sortie est interverrouillée et un guide de lumière doit être connecté et couplé dans un chemin optique
inclus avant l'opération. Chaque commande opérationnelle et l'ouverture laser qui peuvent être séparées de
2 m ou plus à partir d'un dispositif d'avertissement de rayonnement doivent être munies d'un dispositif
d'avertissement de rayonnement. Ne dépassez pas 2m sans fournir un dispositif d'avertissement de
rayonnement conformément à la norme EN 60825-1:2007 & 2014.
Warning: DO NOT open the unit. There are no serviceable parts inside and opening the light engine
enclosure will void the manufacturer’s warranty.
Avertissement: NE PAS ouvrir l’appareil. Il ne contient aucune pièce réparable et l’ouverture de son
boîtier a pour effet d’annuler la garantie.
Caution: DO NOT set liquids on the light engine. Spilled liquids may damage your light engine.
Attention: NE PAS placer de liquide sur la source lumineuse. Les liquides renversés peuvent
endommager la source lumineuse.
Caution: DO NOT drop the light engine. It contains glass optical components that could be damaged or
misaligned by the shock produced by a drop onto a hard surface.
Attention: NE PAS laisser tomber la source lumineuse. Elle contient des composants optiques en verre
susceptibles d’être endommagés ou désalignés par le choc résultant d’une chute sur une surface dure.!
DISCLAIMER: Lumencor shall not be liable for injury to the user or damage to the product
resulting from the SPECTRA III (L) light engine®being used in a way for which it was not intended
and in complete disregard for all posted safety precautions and warnings.
AVIS DE NON-RESPONSABILITÉ: Lumencor décline toute responsabilité pour les blessures
corporelles ou les dommages au produit résultant d’une utilisation du SPECTRA III (L) light
engine®autre que celle prévue et du mépris total de toutes les mesures de sécurité et mises en
garde affichées.
SPECTRA III (L) Light Engine® Instruction Manual
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3. Operating Instructions!
3.1 Contents!
The SPECTRA III (L) light engine ships with the following list of standard components:
1. The SPECTRA III (L) light engine®engine, configured with 8
output channels (colors) and an output adapter for connection
to an SMA-terminated optical fiber or a liquid light guide (LLG) as
documented on the certificate of conformance (Figure 1).
2. A 24 V/9.2 A DC power supply (Lumencor part number
27-10019).
3. A region-specific AC power cord for the power supply (see
adjacent table).
4. RJ45 ethernet cable.
5. Control key, external gate jumper and remote interlock jumper (Figure 2).
6. Quickstart Guide instruction document (85-10049)
!
SPECTRA III (L) Light Engine® Instruction Manual
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AC Power Cords
Region
Part Number
North America
29-10002
Europe
29-10005
United Kingdom
29-10004
Israel
29-10008
Australia/New Zealand
29-10024
Figure 2. Control key, remote interlock jumper and external
gate jumper. These items are packed in a plastic bag inside
the shipping box. Their functions are described in Section
3.3.1
Figure 1. Specimen certificate of conformance (C of C)
for SPECTRA III (L) Light Engine. The C of C identifies
the color channels installed in the light engine and the
bandpass filters associated with each channel. Full
(100%) power outputs measured at the terminus of the
liquid light guide (LLG) or optical fiber are recorded in the
third column.
SPECIMEN

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The model name, unique 4- or 5- digit serial number and certification markings of the light engine are carried
on a label affixed to the rear panel. Performance specifications for individual light engines are listed on the
certificate of conformance included with the shipping documents e-mailed to the customer (Figure 1).
3.2 Installation!
When setting the SPECTRA III (L) light engine up for use, place the unit on a hard surface and avoid blocking
or restricting airflow at the air inlets (front panel; Figure 3) or exhaust ports (rear panel) on the enclosure.
Restricting the airflow will cause the unit to operate at elevated temperatures and will result in decreased
product life and/or premature failure. The SPECTRA III (L) light engine must be operated in a non-
condensing environment (dew point <10ºC with controlled ambient temperature <30º C). Thermal overload
protection is provided by the onboard microprocessor in conjunction with an onboard temperature sensor. If
the internal temperature registered by the sensor exceeds 50°C OR the fan rotor is stopped, all light output
channels automatically turn OFF and are locked in this state until the internal temperature is below 50°C and/
or the fan restarts. The current reading of the onboard temperature sensor is displayed on the front panel
status display (Figure 3) and in the Control GUI
(Section 3.3.3).
3.3 Operation!
3.3.1 Controls and Interlocks!
The Master Power Switch button on the front
panel (Figure 3) turns the electrical power to the
unit on or off. A green power indicator
embedded in the button is lit when the power
supply is connected to the light engine and the
power button is in the on position. Initialization
of the onboard computer takes about 30
seconds after the master power switch is turned
on. When initialization is complete, the status
indicator display (Figure 3) will activate.
The Key Control (Figure 3) must be in the on
position before light output can be turned on.
The key must be removed and stored in a
secure location when the product is not in use.
ONLY trained individuals should use and have
access to the key. The Master Power Switch
button, Key control and Remote interlock
can be used to shut off light output.
The Source Enabled indicator LED (below the
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Class 4 Laser
label
Master
power
switch
Status
indicator
display
Air intake
Figure 3. SPECTRA III (L) light engine front panel.

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status indicator display; Figure 3) provides a warning indication that one or more light sources are active and
emitting invisible and/or visible radiation.
The Remote Interlock Connector: (rear panel, lower left) is provided to allow for connection of a remote
interlock. When this interlock is open it will shut off light output. After the interlock has been opened, the
Manual Interlock Reset button will need to be pushed to resume light output.
Note: In the event of ANY normal or abnormal interlock fault condition (including high ESD/EMP/EFT
conditions ~2kV) you MUST clear the latch fault condition by depressing the manual reset button.
3.3.2 Start Up!
1. Insert the light guide and secure it with the set screw [1,2].
2. Insert the external gate jumper (Figure 2) in the labeled socket on the front panel (Figure 3). Insert the
remote interlock jumper in the labeled socket in the lower left corner of the rear panel.
3. Insert the control key, turn it to the ON position.
4. Connect the isolated DC power supply to the light engine.
5. Connect the AC power cord to the DC power supply.
6. As soon the DC power supply is energized, the master power button (top right) will automatically light up."
The light engine automatically starts when the power is connected; there is no need to push the master
power button [3].
7. Wait 30–45 seconds for the initiation sequence (onboard microprocessor boot-up) to complete."Do not
press any buttons or insert any plugs during this time.
8. When the initiation sequence completes, “LUMENCOR” will flash on front display panel and then be
replaced by a display showing the current light engine IP address, the internal temperature and the fan
status."At the same time, the fan will come on at HI for about 2 seconds and then shut off automatically.
9. The light engine is now ready for use.
Notes
[1] Ensure that the distal end of the light guide or optical fiber is safely directed into an enclosed optical path
(e.g. microscope epilluminator or a beam dump) before turning the light output on. If the light engine is
equipped with an output adapter for an SMA-terminated optical fiber, connect the fiber to the output adapter
using the integral threaded sleeve.
[2] The 3 mm liquid light guide output coupler incorporates an electrical power interlock switch in the back of
the receptacle. The liquid light guide must be fully inserted in the receptacle to activate the interlock.
Electrical power supply to the light engine cannot be turned on via front panel power switch until the interlock
is activated.
[3] For subsequent start ups, use the master power button to start or shut down the light engine. Shut down
can also be accomplished using the “Shut Down” button in the Control GUI (Figure 4).
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3.3.3 Ethernet Connection and Control GUI !
The Web GUI provides a quick and easy way to control the light engine, providing the ability to turn each
source on/off, adjust the power of each source independently from off to full power (Figure 4). The GUI also
tracks the total on time and the power sensor readings. To access the Web GUI on a Windows workstation,
follow the protocol below:
1. Go to Start menu > Control Panel > Network & Internet and/ or Network & Sharing Center
2. Click Change Adapter Settings
3. Right-click on Local Area Connection
4. Click on Properties (in pop-up)
5. Select Internet Protocol Version 4 (TCP/IPv4)
6. Click Properties button
7. Use the following IP addresses:
!IP Address: 192.168.201.201
!Subnet Mask: 255.255.255.0
!Default gate way and DNS Server are OK to leave blank
8. Connect the RJ45 cable (supplied with the light engine) between the LAN port on the light engine and an
ethernet port on the computer.
9. Type the Light Engine IP address (Figure 3) into any web browser address bar to access the resident Web
GUI. The factory default IP address is 192.168.201.200.
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Figure 5. SPECTRA III (L) GUI, Settings Tab. The GUI is
accessed by typing the light engine IP address
(198.168.201.200 in this example) into a web browser.
Figure 4. SPECTRA III (L) GUI, Control Tab. The GUI is
accessed by typing the light engine IP address
(198.168.201.200 in this example) into a web browser.

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3.3.4 Control via Serial Ports!
SPECTRA III (L) light engines have two serial ports, labeled USB and RS-232 (Figure 3), which can be set to
receive either LEGACY or STANDARD mode commands. Connection to the computer requires a USB-A-to-
USB B cable (29-10058) or USB-to-RS-232 cable (29-10011). LEGACY commands are limited to controlling
on/off switching and intensity adjustment of individual color channels. Only one of the two ports can be set
to LEGACY mode at one time. The STANDARD mode command set gives access to an extensive panel of
operating status reports and configuration settings in addition to the the basic control functions of the
LEGACY command set. A complete listing of STANDARD mode commands is provided in Lumencor Light
Engine Command Reference (Document number 57-10018). Note that LEGACY and STANDARD mode
communications use different serial protocols (9600,8,N,1 and 115200,8,N,1 respectively). Changes to the
command mode setting for a serial port can be made via the Settings screen of Web GUI (Figure 5).
Changes are applied instantaneously and are retained between power cycles.
Select the command mode setting for the serial port that is compatible with the light engine device driver in
the control software. This selection is typically found under the “Devices” tab. The COM port address
assigned by the computer to the light engine USB serial port must be correctly registered in control software
at the same place.
3.3.5 Control From Light Engine Control Pod!
1. Connect SPECTRA III (L) to pod (p/n 83-10007) using USB A-to-USB B cable (p/n 29-10058)
2. Open the Web GUI control interface as described in Section 3.3.3.
3. Go to the SETTINGS page in the Web GUI. Make sure that the USB port configuration is set to LEGACY
mode and USB 5V is set to ENABLED.
4. The pod must be set in SPECTRA III (L) control mode. The light engine control mode setting is shown in
green letters at the bottom of the pod display screen. If the pod is not in SPECTRA III (L) control mode,
change the setting by holding down the MODE button on the pod until the light engine selection menu
appears. Move the cursor to “SPECTRA III (L)” by turning the pod control knob. Press the MODE button
again to select SPECTRA III (L) control mode and return to the main control screen.
5. Follow the instructions on Lumencor’s Light Engine Control Pod Operation sheet (p/n 54-10036). In brief,
press the COLOR button to select output light output channel, press the MODE button to toggle light output
on and off and turn the control knob to adjust intensity for the current light output channel selection.
3.3.6 TTL Control!
The TTL Interface provides users with a faster method of switching
color channel outputs on and off. Individual TTL inputs are provided
for each color channel, as shown in the adjacent table. As a
safeguard against unintended light output when the inputs are
initially connected, the light engine TTL port is disabled and must be
enabled by a serial command. To enable the TTL input, click the
Enabled button next to “TTL inputs” in the GUI under the Settings
tab (Figure 5) or send the standard mode command SET
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TTL Connector Pin Definitions
OUTPUT
COLOR
Pin#
OUTPUT
COLOR
Pin#
RED
1
BLUE
12
GREEN
2
VIOLET
13
CYAN
3
nIR
14
TEAL
11
YELLOW
15
Gnd
6,7,8,10
Vihigh (min) = 2.0V, Vihigh (max) = 5.5V
Vilow (min) = 0.0V, Vilow (max) = 0.8V

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TTLENABLE to the LAN port. Input TTL signals can be
conveniently addressed using an accessory BNC breakout cable
(Lumencor part no. 29-10156 or 29-10216; Figure 6) connected to
the front panel TTL port. The breakout cable also provides a global
shutter input (labeled “shutter”). TTL signals input to the global
shutter will synchronously toggle all currently enabled source
channels on and off.
4. Light Output Characteristics!
SPECTRA III (L) light engines may incorporate different types of
solid state light sources. Constituent light sources may include
LEDs, luminescent light pipes or lasers. The light sources installed
and the bandpass filters associated with each are shown on the
Certificate of Conformance included with the shipping documents
e-mailed by Lumencor to the customer. The certificate of
conformance also shows full (100%) power outputs for each
source measured at the terminus of the liquid light guide (LLG) or
optical fiber (Figure 1). Laser sources are listed on a label affixed to
the light engine (see page 7) together with their center wavelength
and maximum output power. Not all sources may be turned on simultaneously. To prevent exceeding the
capacity of the DC power supply, power consumption is tracked by the onboard computer. If a set limit (see
Governor, Figure 5) is exceeded, either by increasing intensity settings for sources that are already on, or by
turning on additional sources, commands will be rejected. To clear the error condition, reduce intensities of
sources that are on or turn off additional sources.
Light source outputs are refined by bandpass filters. The specifications of these bandpass filters are
recorded on the certificate of conformance as CWL/FWHM where CWL =center wavelength and FWHM =
full width at half-maximum transmission, both measure in nanometers (nm). Bandpass filters are not user-
exchangeable and changes require return of the light engine to Lumencor’s factory for service (see Section
7). Since changing bandpass filters will result in source output power changes, a new certificate of
conformance will be provided as part of this service.
SPECTRA III (L) Light Engine® Instruction Manual
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Figure 6. BNC breakout cable (p/n
29-10156). The same cable configuration
with SMB connectors instead of BNC is
also available (29-10216)

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5. Operational Specifications!
The SPECTRA III (L) light engine®must be operated and stored within the environmental conditions specified
in the table below. Performance specifications for individual light engines are listed on the certificate of
conformance included with the shipping documents e-mailed to the customer (see example shown in Figure
1). It is important to retain the certificate of conformance for reference. In the event that the light engine is
sold, the certificate of conformance should be transferred to the new owner. Certificates of conformance are
also recorded in Lumencor’s database and copies can be requested by e-mail to
[e-mail]. The request message must include the 5-digit serial number of the light
engine.
6. Routine Maintenance and Trouble Shooting!
No routine maintenance is required. There are no user-replaceable components or sub-assemblies in the
SPECTRA III (L) light engine. Opening the light engine enclosure will void the manufacturer’s warranty. In the
event that the light engine fails to perform in accordance with the specifications listed on the certificate of
conformance, please contact Lumencor Technical Support for assistance, as directed in Section 7.
SPECTRA III (L) Light Engine® Instruction Manual
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Specification
Detail
Temperature
Operating
32 to 86° F (0 to 30° C)
Non-operating
-4 to 158° F (-20 to 70° C)
Humidity
Operating and non-operating
0 to 80% relative humidity, non-condensing#
Altitude
Operating
0 to 10,000 feet (3,048 meters)
Non-operating
0 to 45,000 feet (13,176 meters)
Dimensions
Size (W x L x H)
145 mm x 340 mm x 203 mm (5.7 in x 13.4 in x 8.0 in)
Weight
8.7 kg /19.1 lbs
Lifetime
Time for light engine output to decrease to 70% of the values
recorded on the original certificate of conformance¶
Power supply
24VDC / 9.2A
Warm-up Period
1 s
Protection
Thermal overload (Section 3.2). Power overdraw (Section 4)
Sound Level
Sound level at 1 meter < 65db(A)
Control Interfaces
USB, RS-232, TCP, TTL
Warranty
18 months parts and labor
# The SPECTRA III (L) light engine must be operated in a non-condensing environment (dew point <10ºC
with controlled ambient temperature <30º C). ¶The corresponding number of days/months/years may vary
considerably depending on the duty cycle implemented by the user and the prevailing environmental
conditions during operation.

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7. Customer Support!
For technical support of the SPECTRA III (L) light engine, please contact Lumencor by phone at
503-213-4269 or through e- mail at [e-mail]. Please be prepared to provide the 5-digit
serial number of the light engine. Any light engine returned to Lumencor for repairs or upgrades requires a
pre-issued return material authorization (RMA) number. To obtain a RMA number, submit the online request
form at http://lumencor.com/support/lumencor-rma-request-form. It is the customer’s responsibility to
properly package and safely ship products to Lumencor. Instructions for shipping will be provided in the e-
mail giving notification of the RMA number.
Troubleshooting Procedures
Problem
Check the following
No response to serial (USB or
RS-232) commands
Check that the LEGACY or STANDARD command mode selection
(GUI Settings; Figure 5) is compatible with the software driver.
No response to TTL trigger
commands
Check that TTL inputs are ENABLED (GUI Settings; Figure 5) and
that the TTL polarity setting is consistent with the trigger inputs.
Also check that all serial ON/OFF controls are in the OFF state.
No light output in response to
source ON command (serial or
TTL).
There are 4 interlocks that need to be closed, check all of these:!
1. Control key must be inserted in front panel and turned to “on”
position¶.!
2. Liquid light guide must be inserted in light output receptacle¶.!
3. Remote interlock jumper must be inserted in rear panel¶.!
4. External gate jumper must be inserted in front panel (Figure 3).
Unusually weak fluorescence
signals across all detection
channels
Weak fluorescence in all detection channels (DAPI, FITC, TRITC,
Cy5 etc) is likely to be due to poor light transmission by the liquid
light guide, the collimating adaptor or another distal component
of the microscope optical path and not to abnormally low light
output from the light engine.
Unusually weak fluorescence
signals in a single detection
channel (e.g. DAPI)
1. Check that the light engine power output reading in the Web
GUI (Figure 4) is normal.!
2. Check that the dichroic beamsplitter and emission bandpass
filter in the microscope are compatible with light engine
excitation filter specifications shown on the certificate of
conformance (Figure 1).
High, spatially uniform
fluorescence background
Check that the dichroic beamsplitter and emission bandpass filter
in the microscope are compatible with light engine excitation filter
specifications shown on the certificate of conformance (Figure 1).
¶ Open interlocks for these conditions will be indicated by a red status indicator in the lower right
corner of the GUI control screen (Figure 4)
SPECTRA III (L) Light Engine® Instruction Manual
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8. Warranty
The SPECTRA III (L) ®family of light engines is backed by a 18 month warranty to end users. Warranty
coverage starts on the original date of shipment from Lumencor. Light Engines qualifying for warranty
service must be verifiably delivering performance that is substantially at variance with the levels documented
in the certificate of conformance. The light engine must also have been used and maintained under
operating conditions consistent with the specifications given in Section 5, and observing all the Precautions
and Warnings notified in Section 2. This warranty does not extend to light engines that have been subject to
misuse, accident, tampering or improper installation. Accessories including (but not limited to) liquid light
guides, optical fibers, collimators, cables and control consoles are not covered by the warranties attached to
light engines. Please fill out and submit the online warranty registration form. This will facilitate provision of
warranty service should it be required.
SPECTRA III (L) Light Engine® Instruction Manual
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