Major Science MJ-105-S User manual

Mini Horizontal
Electrophoresis System
Instruction Manual
Catalog No.
MJ-105-S
MJ-105-R
MT-108
Version 01E
Date: 2014.3.20
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Packing list
MJ-105-S or MJ-105-R:
- 1x Snow Shorter Mini Electrophoresis Tank for MJ-105-S or
1x Red Shorter Mini Electrophoresis Tank for MJ-105-R
-1x Shorter Mini Electrophoresis Cover
-2x Gel Maker Stand
- 2x 107 x 60 mm Tray
- 4x 52 x 60 mm Tray
- 1x Double Side Comb: 6 teeth x 2 & 12 teeth
Prep 1 + Marker 2 & Prep2 + Marker 2 & 23 teeth
- 1x Double Side Comb: 8 teeth x 2 & 17 teeth
12 teeth x 2 & 25 teeth
- 1x Black & Red Power Cord
- 1x Mini Horizontal Electrophoresis System Instruction Manual
MT-108:
- 1x Wide Mini Electrophoresis Tank & Cover
- 1x Gel Maker Stand
- 1x 105 x 83 mm Tray
- 2x 50 x 83 mm Tray
- 1x Double Side Comb: 6 teeth x 2 & 12 teeth
Prep 1 + Marker 2 & Prep2 + Marker 2 & 23 teeth
- 1x Double Side Comb: 8 teeth x 2 & 17 teeth
12 teeth x 2 & 25 teeth
- 1x Black & Red Power Cord
- 1x Mini Horizontal Electrophoresis System Instruction Manual
Signed by:
Date:
Major Science is liable for all missing or damaged parts / accessories
within 7 days after customer receives this instrument package. Please
contact Major Science immediately regarding this issue. If no response is
received within such a time period from consignee party, it will be the
consignee party’s whole responsibility.
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Table of Contents
Packing list...................................................................................................1
Warning ..........................................................................................................3
Section 1 Introduction...............................................................................6
1.1 Overview.............................................................................................6
1.2 Product Description ............................................................................6
Section 2 Technical Specifications..........................................................8
Section 3 Installation Instructions...........................................................9
Section 4 Operation Instructions...........................................................10
4.1 Controls and Features ......................................................................10
4.2 Start the operation.............................................................................10
Section 5 Maintenance............................................................................13
Section 6 Ordering Information..............................................................14
Section 7 Warranty..................................................................................16
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Warning
Major Science Mini Horizontal Gel Electrophoresis System series has been
tested and found to comply with the limits for the CE regulation. Also, Mini
Horizontal Gel Electrophoresis System series is RoHS compliant to deliver
confident product which meets the environmental directive. These limits are
designed to provide reasonable protection against harmful interference when
the instrument series is operated in a commercial environment. This
instrument series used together with power supply unit generates, uses, and
can radiate radio frequency energy, and if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio
communications. Operation of this instrument series in a residential area is
likely to cause harmful interference in which case the user will be required to
correct the interference at their expense. Changes or modifications not
expressly approved by the party responsible for compliance could void the
user’s authority to operate the equipment. It is strongly recommended for the
user to read the following points carefully before operating this equipment.
1. Read and follow the manual instructions carefully.
2. Do not alter the equipment. Failure to follow these directions could result
in personal and/or laboratory hazards, as well as invalidate equipment
warranty.
3. Use a properly grounded electrical outlet with correct voltage and current
handling capacity.
4. Disconnect from power supply before maintenance and servicing. Refer
servicing to qualified personnel.
5. Never use this instrument series without having the safety cover correctly
in position.
6. Do not use the unit if there is any sign of damage to the external tank or
cover. Replace damaged parts.
7. Do not use in the presence of flammable or combustible material; fire or
explosion may result. This device contains components which may ignite
such materials.
8. Refer maintenance and servicing to qualified personnel.
9. Ensure that the system is connected to electrical service according to
local and national electrical codes. Failure to make a proper connection
may create fire or shock hazard.
10. Use appropriate materials and operate correctly to avoid possible hazards
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of explosion, implosion or release of toxic or flammable gases arising from
overheated materials.
11. The unit shall be operated only by qualified personnel.
Safety Information
Use high level of precaution against any electrical device. Before connecting
the electrical supply, check to see if the supply voltage is within the range
stated at the rating label, and see to it that the device be seated firmly. Place
the unit in a safe and dry location; it must NOT touch the surrounding. Follow
the safety precautions for chemicals / dangerous materials. If needed, please
contact qualified service representative or ser[email protected]
Environmental Conditions
Ensure that the instrument is installed and operated strictly under the following
conditions:
1. Indoor use only
2. ≤95% RH
3. 75 kPa –106 kPa
4. Altitude must not exceed 2000 meters
5. Ambient to 40°C operating temperature
6. Pollution degree: 2
7. Mains supply voltage fluctuations up to ±10% of the normal voltage
Avoiding Electrical Shock
Follow the guidelines below to ensure safe operation of the unit.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power supply unit when
the power supply unit is still on.
2. WAIT at least 5 seconds after stopping a run before handling output leads
or connected apparatus.
3. ALWAYS make sure that hands, work area, and instruments are clean and
dry before making any connections or operating the power supply.
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Avoiding Damage to the Instrument
1. Do not attempt to operate the device if damage is suspected.
2. Protect this unit from physical damage, corrosive agents and extreme
temperatures (direct sunlight, etc.).
3. For proper ventilation and safety concerns, keep at least 10 cm of space
behind the instrument, and at least 5 cm of space on each side.
4. Use high level of precautions against the damages on the unit.
5. Do not operate the unit out of environmental conditions addressed above.
6. Prior to applying any cleaning or decontamination methods other than
manufacturer’s recommendation, users should check with the
manufacturer’s instruction to see if the proposed method will damage the
equipment.
Equipment Operation
Follow the guidelines below to ensure safe operation of the unit:
1. NEVER access dangerous chemicals or other materials to prevent possible
hazard of explosion and damage.
2.Do not operate the unit without lids or covers to prevent possible hazards.
3. A temporary conductivity caused by condensation might occur even though
this series is rated Pollution Degree 2 in accordance with IEC 664.
Symbol
The symbol used on Mini Horizontal Electrophoresis System series is
explained below.
Indicates an area where a potential shock hazard may exist.
Consult the manual to avoid possible personal injury or instrument
damage.
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Section 1 Introduction
1.1 Overview
The Major Science Mini Horizontal Electrophoresis System series has a
comprehensive design for several kinds of applications. Its excellent and
consistent performance time after time delivers accurate and reliable
experimental results. Furthermore, this series is also offered with the Major
Science Minis Power Supply series as packages with even more competitive
pricing offerings and great value as well as convenience to the user. More
importantly, the Mini Horizontal Electrophoresis System series is RoHS-
compliant and designed to comply with CE regulations.
1.2 Product Description
The MJ-105-S, MJ-105-R, and MT-108 Mini Horizontal Gel Electrophoresis
System series is designed for very fast and clear separation of DNA or RNA
restriction fragments. This series is offered as a complete system, including all
the necessary accessories for the user to cast, run and perform
electrophoresis. It is constructed of polycarbonate to withstand up to 130°C. It
is a single molded unit designed to prevent cracks or leakages. This simple
operated gel casting system and electrophoresis system maximizes the
consistency of each procedure. The safety interlock cover is designed to
prevent electrical hazards during electrophoresis.
Features:
- Less agarose consumption
- Less running buffer consumption
- Multichannel pipette compatible
- Single molded tank
- Two tray options available
- Safety & Ventilation Cover/Lid
- Contain 2 sets of gel maker stand for MJ-105-S and MJ-105-R
Contain 1 set of gel maker stand for MT-108
- Cast 6 pcs of agarose gel in one time for MJ-105-S and MJ-105-R
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Cast 3 pcs of agarose gel in one time for MT-108
- High temperature capability, 130°C
- Easy sample loading
- Great indications for gel-making and running
- No tapes, clamps or springs needed
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Section 2 Technical Specifications
MJ-105-S and MJ-105-R
MJ-105-S Appearance
1 x snow tank, 1 x transparent lid, 2 x gel
maker stand, 6 x trays, 2 x combs, 1 x
black & Red power cords
MJ-105-R Appearance
1 x transparent red tank, 1 x transparent
lid, 2 x gel maker stand, 6 x trays, 2 x
combs, 1 x black & Red power cords
Construction Material (Tank, Gel
Maker Stand, Comb and Tray)
PC (Polycarbonate)
Construction Material
(Transparent Lid)
AS (Acrylonitrile-Styrene Copolymer)
Temperature Capacity
130°C
Unit Dimension (Tank and
Transparent Lid)
140 x 140 x 53 mm (W x L x H)
Gel Dimension (Tray)
52 x 60 mm
107 x 60 mm
Maximum Gel Thickness
10 mm
Maximum sample
25 samples
Buffer Volume
Approx. 200 ml
Transparent Lid
Safety and ventilation design
Tray
Black well-visualization strip
Migration distance index line
Agarose level, 5 mm
Rapid Casting Gel
Use gel maker stand
Operating Temperature
4 - 40°C
Weight
Approx. 0.5 kg
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MT-108
Construction Material (Tank, Gel
Maker Stand, Comb and Tray)
PC (Polycarbonate)
Construction Material
(Transparent Lid)
AS (Acrylonitrile-Styrene Copolymer)
Temperature Capacity
130°C
Maximum Gel Thickness
10 mm
Maximum sample for MT-108
25 samples
Unit Dimension (Tank and
Transparent Lid)
136 x 188 x 58 mm (W x L x H)
Gel Dimension for MT-108
50 x 83 mm
105 x 83 mm
Buffer Volume
Approx. 400 ml
Transparent Lid
Safety and ventilation design
Tray
Black well-visualization strip
Migration distance index line
Rapid Casting Gel
Use gel maker stand
Operating Temperature
4 - 40°C
Weight
0.7 kg
Section 3 Installation Instructions
1.Seat the tray in the unit and note the position of the comb grooves. The
samples run black to red but the trays can be used in the forward and
backward direction. So make sure that the comb grooves closest to the black
electrode are marked.
2.Remove the tray.
3.Note the position of the cover on the unit. This indicates the correct polarity
and the correct orientation of the power codes: black is negative and red is
positive.
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Section 4 Operation Instructions
4.1 Controls and Features
MJ-105-S MJ-105-R
MT-108
4.2 Start the operation
Gel Preparation
1.Add the appropriate amount of agarose powder and 1x TAE or 1x TBE
solution into a glass flask or beaker based on desired ratio.
2.Dissolve the agarose powder by heating the agarose either on a magnetic
hot plate with a stirring bar or in a microwave oven. If using the microwave
method, the microwave should be set at around 400W or medium setting
Tank
Cover
Black & Red Power Cord
50 x 83 mm Tray
Analytical Comb
Gel Maker Stand
105 x 83 mm Tray
52 x 60 mm Tray
Analytical comb
Analytical comb
107 x 60 mm Tray
Tank
Cover
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with the flask swirling once every minute. The solution should be heated until
all crystals are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere with sample
migration if not completely dissolved.
Note: The gel must be cooled to between 50°C and 60°C degrees before
pouring.
3.Install the Gel Tray in the Gel Maker Stand.
4.Select the suitableAnalytical Comb for the application.
5.Pour approx. 25 to 30 ml of agarose solution for a large tray; pour 12 to 15
ml for a small tray. The tray contains the agarose level for reference.
Note: Pour the agarose carefully so as not to generate bubbles. Any bubble
that surfaces could be smoothed to the edge of the gel and dispersed
using a pipette tip.
6.Place the comb(s) into the grooves.
7.As for the MJ-105 series, both Gel Maker Stands can be connected together
for user convenience.
Gel Running
1.Carefully remove the comb and transfer the gel, including the tray, to the
electrophoresis tank.
2.Refer to the molecular biology protocols for sample preparation.
3.Fill the electrophoresis tank with buffer until the gel is just filled to the top.
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This will give the fastest resolution times. For enhanced quality of resolution
of the sample, we suggest filling the electrophoresis tank to 5mm above the
gel.
4.Load the samples into the wells, and then attach the safety ventilation cover.
Finally connect the power cords to the power supply unit.
5.Turn on the power supply unit and adjust to the desired electrophoresis
running conditions.
6.When the electrophoresis running is complete, turn the power supply unit off
first and then disconnect the power cords and remove the safety cover.
Note: Ensure that the power supply unit is turned off before assembly or
disassembly of any electrophoresis unit.
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Section 5 Maintenance
The Major Science Horizontal Electrophoresis System series can be cleaned
with warm water (below 40°C) with a mild detergent no more than 20 minutes.
After that, rinse with distilled water. Air-dry before use. Some cleaning agents
are not supposed to come in contact with the electrophoresis systems, such as
Acetone, Phenol, Chloroform, Alcohol and Alkalis.Contact Major Science for
details and suggestions.
* Trays are particularly vulnerable to scratch. Make sure cleaning with care.
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Section 6 Ordering Information
Cat. No.
Description
MJ-105-S
Snow Mini Horizontal Electrophoresis System
MJ-105-R
Red Mini Horizontal Electrophoresis System
MT-108
Wide Mini Horizontal Electrophoresis System
Single Electrophoresis Packages
MJS-100P
Package of MJ-105-S and MP-100
MJR-100P
Package of MJ-105-R and MP-100
MT-100P
Package of MT-108 and MP-100
MJS-Mini300
Package of MJ-105-S and Mini-300
MJR-Mini300
Package of MJ-105-R and Mini-300
MT-Mini300
Package of MT-108 and Mini-300
Dual Electrophoresis Packages
MJSS-Mini300 Package of 2 x MJ-105-S and Mini-300
MJRR-Mini300
Package of 2 x MJ-105-R and Mini-300
MTT-Mini300
Package of 2 x MT-108 and Mini-300
MJSR-Mini300
Package of MJ-105-S & MJ-105-R and Mini-300
MJST-Mini300
Package of MJ-105-S & MT-108 and Mini-300
MJRT-Mini300
Package of MJ-105-R & MT-108 and Mini-300
ACCESSORIES
MJ-G01
Gel Maker Stand
MJ-T01
52 x 60 mm Tray
MJ-T02
107 x 60 mm Tray
MJ-C01
Analytical Comb
8 teeth x 2 for mini gel & 17 teeth for midi gel
12 teeth x 2 for mini gel & 25 teeth for midi gel
MT-C03
Analytical Comb
6 teeth x 2 for mini gel & 12 teeth for midi gel
Prep 1 + Marker 2 & Prep 2 + Marker 2 for mini gel & 23 teeth for
midi gel
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MT-P01
Power Cord (Black & Red)
MJ-CASTER-C
contains: 2 x MJ-G01; 4 x MJ-T01; 2 x MJ-02 and 2 x MJ-C01
MT-CASTER-C
contains: 1 x MT-G01; 1 x MT-T01; 2 x MT-02; 1 x MT-C03 and 1 x
MJ-C01
Single Electrophoresis Packages
MJS-Mini300 MJR-Mini300 MT-Mini300
Dual Electrophoresis Packages
MJSS-Mini300 MJRR-Mini300 MJSR-Mini300
MJST-Mini300 MJRT-Mini300 MTT-Mini300
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Section 7 Warranty
Major Science warrants apparatus of its manufacture against defects in
materials and workmanship, under normal service, for one year from the
shipping date to purchaser. This warranty excludes damages resulting from
shipping, misuse, carelessness, or neglect. Consumable parts (platinum wire)
are not covered by our warranty. Major Science’s liability under the warranty is
limited to the receipt of reasonable proof by the customer that the defect is
embraced within the terms of the warranty. All claims made under this warranty
must be presented to Major Science within one year following the date of
delivery of the product to the customer.
Manufacturer:
Major Science Co., Ltd.
Address:
3F, No.37, Wuguan 5th Rd.,
Wugu Dist., New Taipei City 24888,
Taiwan
T/ 886-2-2298-1055
F/ 886-2-2299-7871
Contact Information
Address:
19959 Sea Gull Way
Saratoga, CA 95070
U.S.A
T/ 1-408-366-9866
F/ 1-408-446-1107
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MEMO
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MEMO
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MEMO
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