Major Science MJ-105A User manual

Mini Horizontal
Electrophoresis System
Instruction Manual
Catalog No. MJ-105A
MT-108
www.majorsci.com
[e-mail]
Version: 10E
Date: 2018/11/29
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Packing list
MJ-105A:
- 1x Transparent Shorter Mini Electrophoresis Tank & Lid
- 2x Gel Maker Stand
- 2x 107 x 60 mm Tray
- 4x 52 x 60 mm Tray
- 1x Double Side Comb: 6 teeth x 2 & 12 teeth
Prep 1 + Marker 2 & Prep2 + Marker 2 & 23 teeth
- 1x Double Side Comb: 8 teeth x 2 & 17 teeth
12 teeth x 2 & 25 teeth
- 1x Black & Red Power Cord
- 1x Instruction Manual
MT-108:
- 1x Wide Mini Electrophoresis Tank & Lid
- 1x Gel Maker Stand
- 1x 105 x 83 mm Tray
- 2x 50 x 83 mm Tray
- 1x Double Side Comb: 6 teeth x 2 & 12 teeth
Prep 1 + Marker 2 & Prep2 + Marker 2 & 23 teeth
- 1x Double Side Comb: 8 teeth x 2 & 17 teeth
12 teeth x 2 & 25 teeth
- 1x Black & Red Power Cord
- 1x Instruction Manual
Signed by:
Date:
Major Science is liable for all missing or damaged parts / accessories
within 7 days after customer receives this instrument package. Please
contact Major Science immediately regarding this issue. If no response is
received within such a time period from consignee party, it will be the
consignee party’s whole responsibility.
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Table of Contents
Packing list....................................................................................................1
Warning.........................................................................................................3
Section 1 Introduction ..........................................................................6
1.1 Overview ...........................................................................................6
Section 2 Technical Specifications .....................................................8
Section 3 Installation Instructions......................................................9
Section 4 Operation Instructions......................................................10
4.1 Controls and Features....................................................................10
4.2 Start the operation .........................................................................11
Section 5 Maintenance........................................................................15
Section 6 Ordering Information........................................................16
Section 7 Warranty..............................................................................19
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Warning
Major Science Mini Horizontal Gel Electrophoresis System series has been
tested and found to comply with the limits for the CE regulation. Also, Mini
Horizontal Gel Electrophoresis System series is RoHS compliant to deliver
confident product which meets the environmental directive. These limits are
designed to provide reasonable protection against harmful interference when
the instrument series is operated in a commercial environment. This
instrument series used together with power supply unit generates, uses, and
can radiate radio frequency energy, and if not installed and used in accordance
with the instruction manual, may cause harmful interference to radio
communications. Operation of this instrument series in a residential area is
likely to cause harmful interference in which case the user will be required to
correct the interference at their expense. Changes or modifications not
expressly approved by the party responsible for compliance could void the
user’s authority to operate the equipment. It is strongly recommended for the
user to read the following points carefully before operating this equipment.
1. Read and follow the manual instructions carefully.
2. Do not alter the equipment. Failure to follow these directions could result
in personal and/or laboratory hazards, as well as invalidate equipment
warranty.
3. Use a properly grounded electrical outlet with correct voltage and current
handling capacity.
4. Disconnect from power supply before maintenance and servicing. Refer
servicing to qualified personnel.
5. Never use this instrument series without having the safety cover correctly
in position.
6. Do not use the unit if there is any sign of damage to the external tank or
cover. Replace damaged parts.
7. Do not use in the presence of flammable or combustible material; fire or
explosion may result. This device contains components which may ignite
such materials.
8. Refer maintenance and servicing to qualified personnel.
9. Ensure that the system is connected to electrical service according to
local and national electrical codes. Failure to make a proper connection
may create fire or shock hazard.
10. Use appropriate materials and operate correctly to avoid possible hazards
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of explosion, implosion or release of toxic or flammable gases arising from
overheated materials.
11. The unit shall be operated only by qualified personnel.
Safety Information
Use high level of precaution against any electrical device. Before connecting
the electrical supply, check to see if the supply voltage is within the range
stated at the rating label, and see to it that the device be seated firmly. Place
the unit in a safe and dry location; it must NOT touch the surrounding. Follow
the safety precautions for chemicals / dangerous materials. If needed, please
contact qualified service representative or [e-mail]
Environmental Conditions
Ensure that the instrument is installed and operated strictly under the following
conditions:
1. Indoor use only
2. ≤95% RH
3. 75 kPa – 106 kPa
4. Altitude must not exceed 2000 meters
5. Ambient to 40°C operating temperature
6. Pollution degree: 2
7. Mains supply voltage fluctuations up to ±10% of the normal voltage
Avoiding Electrical Shock
Follow the guidelines below to ensure safe operation of the unit.
To avoid electrical shock:
1. NEVER connect or disconnect wire leads from the power supply unit when
the power supply unit is still on.
2. WAIT at least 5 seconds after stopping a run before handling output leads
or connected apparatus.
3. ALWAYS make sure that hands, work area, and instruments are clean and
dry before making any connections or operating the power supply.
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Avoiding Damage to the Instrument
1. Do not attempt to operate the device if damage is suspected.
2. Protect this unit from physical damage, corrosive agents and extreme
temperatures (direct sunlight, etc.).
3. For proper ventilation and safety concerns, keep at least 10 cm of space
behind the instrument, and at least 5 cm of space on each side.
4. Use high level of precautions against the damages on the unit.
5. Do not operate the unit out of environmental conditions addressed above.
6. Prior to applying any cleaning or decontamination methods other than
manufacturer’s recommendation, users should check with the
manufacturer’s instruction to see if the proposed method will damage the
equipment.
Equipment Operation
Follow the guidelines below to ensure safe operation of the unit:
1. NEVER access dangerous chemicals or other materials to prevent possible
hazard of explosion and damage.
2. Do not operate the unit without lids or covers to prevent possible hazards.
3. A temporary conductivity caused by condensation might occur even though
this series is rated Pollution Degree 2 in accordance with IEC 664.
Symbol
The symbol used on Mini Horizontal Electrophoresis System series is
explained below.
Indicates an area where a potential shock hazard may exist.
Consult the manual to avoid possible personal injury or instrument
damage.
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Section 1 Introduction
1.1 Overview
The Major Science Mini Horizontal Electrophoresis System series has a
comprehensive design for several kinds of applications. Its excellent and
consistent performance delivers accurate and reliable experimental results.
Furthermore, this series is also offered with the Major Science Minis Power
Supply series as packages with competitive pricing offerings and great value
as well as convenience to the user. More importantly, the Mini Horizontal
Electrophoresis System series is RoHS- compliant and designed to comply
with CE regulations.
Major Science Mini Horizontal Electrophoresis System series offers many
advantages for nucleic acid separation. Agarose gels are convenient to cast
and samples are easy to load. The moulded design prevents cracks and
leakages and the sturdy PC provides a 130°C temperature capacity. The
safety interlock cover is designed to prevent electrical hazards during
electrophoresis. It is ideal for several applications, including Northern and
Southern blotting, Cosmid library restriction analysis, STS screening,
microsatellite analysis, PCR fragment analysis, RFLP analysis, DNA
finger-printing and high throughput analysis.
Features:
Less agarose consumption
Less running buffer consumption
Multichannel pipette compatible
Single molded tank
Safety & Ventilation Lid
High temperature capability, 130°C
Easy sample loading
Great indications for gel-making and running
No tapes, clamps or springs needed
No direct heat impact from power supply unit
MJ-105A additional features:
Transparent tank and lid
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Real time observation with Bluview Transilluminator—MBE-300
Contain 2 sets of gel maker stand
Cast 6 pcs of agarose gel in one time
MT-108 additional features:
Longer tray allows running of bigger fragments to attain higher resolution.
Two tray options available
Contain 1 set of gel maker stand
Cast 3 pcs of agarose gel in one time
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Section 2 Technical Specifications
Catalog No.
MJ-105A
MT-108
Appearance Transparent tank &
transparent lid Snow tank & transparent lid
Material (Tank, Gel Maker
Stand, Comb and Tray) PC (Polycarbonate)
Material
(Lid) PC (Polycarbonate) AS (Acrylonitrile-Styrene
Copolymer)
Temperature Capacity
130°C
Gel (Tray) Dimension (W x L)52 x 60 mm 50 x 83 mm
107 x 60 mm 105 x 83 mm
Maximum Gel Thickness 10mm
Maximum sample
25 samples
Buffer Volume
Approx. 200 ml Approx. 400 ml
Lid
Safety and ventilation design
Tray Black well-visualization strip
Migration distance index line
Agarose level, 5 mm
Rapid Casting Gel
Use gel maker stand
Operating Temperature
4 - 40°C
Dimension
(W x L x H)
148.3x 145.8 x 59.1 mm 136 x 188 x 58 mm
Weight
Approx. 0.5 kg 0.7 kg
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Section 3 Installation Instructions
1. Seat the tray in the unit and note the position of the comb grooves. The
samples run black to red but the trays can be used in the forward and
backward direction. So make sure that the comb grooves closest to the black
electrode are marked.
2. Remove the tray.
3. Note the position of the cover on the unit. This indicates the correct polarity
and the correct orientation of the power codes: black is negative and red is
positive.
For MJ-105A:
Screw black and red cable onto the cover first. Then put it onto the tank.
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Section 4 Operation Instructions
4.1 Controls and Features
MJ-105A
MT-108
Tank
Lid
Black & Red Power Cord
50 x 83 mm Tray
Analytical Comb
Gel Maker Stand 105 x 83 mm Tray
52 x 60 mm Tray
Analytical comb
107 x 60 mm Tray
Tank
Lid
Black & Red Power Cord
Gel Maker Stand
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4.2 Start the operation
Gel Preparation
1.Add the appropriate amount of agarose powder and 1x TAE or 1x TBE
solution into a glass flask or beaker based on desired ratio.
2.Dissolve the agarose powder by heating the agarose either on a magnetic
hot plate with a stirring bar or in a microwave oven. If using the microwave
method, the microwave should be set at around 400W or medium setting
with the flask swirling once every minute. The solution should be heated until
all crystals are dissolved. This is best viewed against a light background.
Crystals appear as translucent crystals. These will interfere with sample
migration if not completely dissolved.
Note: The gel is suggested to be cooled to between 50°C and 60°C degrees before
pouring.
3.Install the Gel Tray in the Gel Maker Stand.
4.Select the suitable Analytical Comb for the application.
5.Pour approx. 25 to 30 ml of agarose solution for a large tray; pour 12 to 15
ml for a small tray. The tray contains the agarose level for reference.
Note: Pour the agarose carefully so as not to generate bubbles. Any bubble that
surfaces could be smoothed to the edge of the gel and dispersed using a
pipette tip.
6.Place the comb(s) into the grooves.
7.As for the MJ-105 series, both Gel Maker Stands can be connected together
for user convenience.
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Gel Running
1.Carefully remove the comb and transfer the gel, including the tray, to the
electrophoresis tank.
2.Refer to the molecular biology protocols for sample preparation.
3.Fill the electrophoresis tank with buffer until the gel is just filled to the top.
This will give the fastest resolution times. For enhanced quality of resolution
of the sample, we suggest filling the electrophoresis tank to 5mm above the
gel.
4.Load the samples into the wells, and then attach the safety ventilation cover.
Finally connect the power cords to the power supply unit.
5.Turn on the power supply unit and adjust to the desired electrophoresis
running conditions.
6.When the electrophoresis running is complete, turn the power supply unit off
first and then disconnect the power cords and remove the safety cover.
Note: Ensure that the power supply unit is turned off before assembly or disassembly
of any electrophoresis unit.
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Gel Observation (with MBE-300)
1.Before running gel electrophoresis, remove the foot stands of MBE-300
and slide MBE-300 under the electrophoresis tank.
2.Place amber filter lid onto the cover of the electrophoresis rank and
connect the fan cable in case condensation forms under the cover and
affects the observation.
Caution: The fan is NOT water-proof, please be careful not to spill liquid
onto the fan. Otherwise the fan may malfunction.
3.Switch MBE-300 on whenever users would like to observe the gel.
4.For capturing images, it is recommended to remove the cover of MJ-105A
and place amber filter lid onto the electrophoresis tank.
↑
Magnetic foot stand
Note: The above picture, gel preparation and gel running instruction serves only as
suggestion. Please do the experiment base on user’s need.
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Gel Image Capture (with MBE-300 and MBE-HOOD)
1. After running gel electrophoresis, place the DNAgel onto the center of
MBE-300 to capture clearer images.
2. Switch MBE-300 on and place MBE-HOOD to cover MBE-300 and a DNA
gel.
3. Observe and capture images of a DNA gel through the amber filter on the
top of MBE-HOOD.
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Section 5 Maintenance
The Major Science Horizontal Electrophoresis System series can be cleaned
with warm water (below 40°C) with a mild detergent no more than 20 minutes.
After that, rinse with distilled water. Air-dry before use. Some cleaning agents
are not supposed to come in contact with the electrophoresis systems, such as
Acetone, Phenol, Chloroform, Alcohol and Alkalis.If you have any question,
contact Major Science for more detail and suggestion.
* Trays are particularly vulnerable to scratch. Make sure cleaning with care.
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Section 6 Ordering Information
For MJ-105A
Cat. No.
Description
MJ
-105ATransparent Mini Horizontal Electrophoresis System
Single Electrophoresis Packages
MJ
A-100P Package of MJ-105A and MP-100
MJ
A-Mini300 Package of MJ-105A and Mini-300
MJAE
-100P Package of MJ-105A, MP-100, and MBE-300; amber filter lid with fan,
synthetic paper hood are included
MJAE
-Mini300 Package of MJ-105A, MINI-300, and MBE-300; amber filter lid with
fan, synthetic paper hood are included
MJA
-MBEP Package of MJ-105A and MBE-300; amber filter lid with fan, synthetic
paper hood are included
Dual Electrophoresis Packages
MJ
AA-
Mini300
Package of 2 x MJ-105A and Mini-300
MJ
AT-Mini300 Package of MJ-105A & MT-108 and Mini-300
For MT-108
Cat. No.
Description
MT
-108 Wide Mini Horizontal Electrophoresis System
Single Electrophoresis Packages
MT
-100P Package of MT-108 and MP-100
MT
-Mini300 Package of MT-108 and Mini-300
Dual Electrophoresis Packages
M
TT-Mini300 Package of 2 x MT-108 and Mini-300
MJ
AT-Mini300 Package of MJ-105A & MT-108 and Mini-300
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ACCESSORIES
Cat. No.
Description
MJ
-B01-A Transparent tank and lid, without accessories
MJ
-LID-AM580 580nm amber filter lid with fan, suitable for MJ-105A
MBE
-LID-AM580 An inclined 580nm amber filter, suitable for MBE-200 and
MBE-300
MBE
-HOOD Synthetic paper hood
MJ
-G01 Gel Maker Stand
MJ
-T01 52 x 60 mm Tray
MJ
-T02 107 x 60 mm Tray
MJ
-C01 Analytical Comb
8 teeth x 2 for mini gel & 17 teeth for midi gel
12 teeth x 2 for mini gel & 25 teeth for midi gel
MT
-C03 Analytical Comb
6 teeth x 2 for mini gel & 12 teeth for midi gel
Prep 1 + Marker 2 & Prep 2 + Marker 2 for mini gel & 23 teeth for
midi gel
MBE
-150-CABLE Red/ Black cable
MJ
-CASTER-C 2 x MJ-G01; 4 x MJ-T01; 2 x MJ-02 and 2 x MJ-C01
MT
-CASTER-C 1 x MT-G01; 1 x MT-T01; 2 x MT-02; 1 x MT-C03 and 1 x MJ-C01
For MJ-105A
Single Electrophoresis Packages
MJA-100P MJA-Mini300
MJAE-100P MJAE-Mini300 MJA-MBEP
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Dual Electrophoresis Packages
For MT-108
Single Electrophoresis Packages
Dual Electrophoresis Packages
MT-Mini300
MJAT-Mini300
MJAA-Mini300
MJAT-Mini300
MTT-Mini300
MT-100P
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Section 7 Warranty
Major Science warrants apparatus of its manufacture against defects in
materials and workmanship, under normal service, for one year from the
shipping date to purchaser. This warranty excludes damages resulting from
shipping, misuse, carelessness, or neglect. Consumable parts (platinum wire)
are not covered by our warranty. Major Science’s liability under the warranty is
limited to the receipt of reasonable proof by the customer that the defect is
embraced within the terms of the warranty.All claims made under this warranty
must be presented to Major Science within one year following the date of
delivery of the product to the customer.
Manufacturer:
Major Science Co., Ltd.
Address:
No.19, Ln. 207, Huakang St.
Bade Dist., Taoyuan City 33464
Taiwan
T/ 886-3-362-3319
F/ 886-3-362-3133
Contact Information
Address:
19959 Sea Gull Way
Saratoga, CA 95070
U.S.A
T/ 1-408-366-9866
F/ 1-408-446-1107
DOMINIQUE DUTSCHER SAS