Merck Millipore Immobilon-E User manual

SigmaAldrich.com
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Quick Start Guide
Immobilon®NOW Transfer Membrane Dispenser
IMDISP
For information on using Immobilon®transfer
membrane, go to SigmaAldrich.com/Immobilon.
The Immobilon®NOW Dispenser facilitates
rapid, precise measurement and cutting of
transfer membranes to standard mini and midi
sizes. It is designed for use with the following
Immobilon®transfer membrane products:
Transfer Membrane Core Color Cat. No.
Immobilon®-E Yellow IEVH85R
Immobilon®-FL Green IPFL85R
Immobilon®-P Blue IPVH85R
Immobilon®-PSQ Red ISEQ85R
Immobilon®-NC* Blue HATF85R
*Included in the narrow roll box you will find a
membrane ID card along with a hazard sticker. Insert
the membrane ID card into the pouch on the inside
of the dispenser top. Ensure the hazard sticker
is displayed in a visible location and the dispenser
is stored appropriately. See the Immobilon-NC SDS
for details, located on the product page
at SigmaAldrich.com.
Knob
Slot
Cover
Core
Threaded hole
Membrane ID card
Membrane
roll
NOTE: Assembly drawing does not depict all graphics
appearing on the dispenser.
1. Lift cover of dispenser. Hold the core and unscrew
the black knob.
2. Remove core from dispenser.
3. Remove outer packaging from the roll of membrane,
orient roll as shown, and insert the core into the roll.
CAUTION: The membrane is sandwiched between
blue patapar sheets. To avoid damaging it, leave the
patapar in place while dispensing and cutting.
4. Slide the core and membrane roll into place in the
side grooves and screw the knob in to secure the
roll in the dispenser.
5. Detach the appropriate Immobilon®membrane
ID card from the strip of cards and fill in the lot
number. Place the card in the pouch on the inside
of the dispenser top.Remove tape from the end of
the membrane roll and feed the patapar/membrane
sandwich up through the slot in the cover and out
towards the front of the dispenser.
Slot under bar
Pull
membrane
forward
to edge
Scissor grooves
6. Gently pull the membrane/patapar sandwich
forward as indicated and align the edge with the
front edge of the cover (marked “ALIGN MEMBRANE
TO EDGE”).

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Merck, Millipore, Immobilon, and Sigma-Aldrich are trademarks of
Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks
are the property of their respective owners. Detailed information on
trademarks is available via publicly accessible resources.
© 2022 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.
7. The cover has grooves to position the
scissors for cutting membrane to fit standard
mini (7.8 × 8.5 cm) or midi (8.5 × 13.5 cm) gels.
With the membrane flat against the cover, position
scissors in one of the cover grooves to cut the
desired length.
8. After cutting, partially retract the membrane back
into the dispenser, leaving just enough at the slot
to pull it forward.
Retract
membrane
Notice
We provide information and advice to our customers
on application technologies and regulatory matters to
the best of our knowledge and ability, but without
obligation or liability. Existing laws and regulations are
to be observed in all cases by our customers. This also
applies in respect to any rights of third parties. Our
information and advice do not relieve our customers
of their own responsibility for checking the suitability
of our products for the envisaged purpose.
The information in this document is subject to change
without notice and should not be construed as a
commitment by the manufacturing or selling entity,
or an affiliate. We assume no responsibility for any
errors that may appear in this document.
Technical Assistance
Visit the tech service page on our web site at
SigmaAldrich.com/TechService.
Terms and Conditions of Sale
Warranty, use restrictions, and other conditions of sale
may be found at SigmaAldrich.com/Terms.
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For the location of the office nearest you, go to
SigmaAldrich.com/Offices.