Merck Millipore ma700 User manual

SigmaAldrich.com

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Introduction

The mA700 Essential Power Supply is microprocessor

controlled and designed to meet most electrophoresis

needs. This manual describes the setup and operation

of the power supply, including important information

on safety and maintenance. The mA700 Essential Power

Supply can run horizontal & vertical electrophoresis,

SDS-PAGE, native PAGE, two-dimensional

electrophoresis, and electro-blotting.

The power supply offers access to 4 terminal pairs,

enabling use with multiple electrophoresis devices

simultaneously. The mA700 Essential Power Supply

provides constant voltage, constant current or constant

power.

Features of mA400 Basic Power Supply:

• Compact size

• Microprocessor controller

• Constant voltages, constant currents and constant

power

• Four terminal outlets

• LCD display

• Timer with alarm function

• Advanced safety devices

• Stackable

• Wide applications for DNA, RNA, and protein

electrophoresis

User Guide

mA700 Essential Power Supply

mA700

Introduction ....................1

Equipment Operation .............2

Symbols Used on Device

Regulatory

Warnings

Safety Precautions

Environmental Operating Conditions

Avoiding Electrical Shock

Avoiding Damage to the Instrument

Technical Specifications ...........3

Installation Instructions ..........4

Operating Instructions

Control Interface

Button Functions

General Operating Instructions .....5

Constant Mode Setup

Constant Mode Operation

Program Mode Setup

Program Mode Operation

Replacing the Fuse

Troubleshooting and Maintenance ...7

Product Ordering Information ......9

Support........................9

Notice

Contact Information

Legal Manufacturer

Technical Assistance

Standard Warranty

Back View

Front View

Main Power Socket

Fuse ON/OFF Switch

Label

Fan

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Equipment Operation

Symbols Used on Device

The following symbols are used on this device,

throughout this user guide and/or on product labels,

and the user shall abide by indicated requirements.

Symbol Definition

Warning alerts you to actions that may cause

personal injury or pose a physical threat.

Indicates an area where a potential shock hazard

may exist. Consult the user guide to avoid possible

personal injury or instrument damage.

Do not discard with common solid waste at end

of life. Segregate with other waste electrical and

electronic equipment (WEEE) and send to an

appropriate facility for recycling. For information on

recycling electrical and electronic products in the

European Union, please visit

SigmaAldrich.com/weee.

Regulatory

The mA700 Essential Power Supply has been CE

marked to reflect compliance to the necessary

European Directives and NRTL (Nationally Recognized

Testing Laboratory) marked under the requirements of

OSHA (Occupational Safety and Health Organization)

for North America. For additional details into our

compliance claims, please refer to the product’s

web page online at SigmaAldrich.com. Search for

the product page, which shows the Declaration of

Conformity.

Warnings

The power supply unit generates, uses, and can radiate

radio frequency energy, and if not installed and used

in accordance with the user guide, may cause harmful

interference to radio communications. Operation of the

mA700 Essential Power Supply in a residential area

is likely to cause harmful interference in which case

the user will be required to correct the interference at

their expense. Changes or modifications not expressly

approved by the party responsible for compliance could

void the user’s authority to operate the equipment.

It is strongly recommended for the user to read

the following points carefully before operating this

equipment.

• Do not alter the equipment. Failure to follow these

directions could result in personal and/or laboratory

hazards, as well as invalidate equipment warranty.

• Use a properly grounded electrical outlet with

correct voltage and current handling capacity.

• Disconnect from power supply before maintenance

and servicing. Refer servicing to qualified

personnel.

• Never use this instrument series without having the

safety cover correctly in position.

• Do not use the unit if there is any sign of damage

to the external tank or cover. Replace damaged

parts.

• Do not use in the presence of flammable or

combustible material; fire or explosion may result.

This device contains components which may ignite

such materials.

• Refer maintenance and servicing to qualified

personnel.

• Ensure that the system is connected to electrical

service according to local and national electrical

codes. Failure to make a proper connection may

create fire or shock hazard.

Safety Precautions

Take a high level of precaution when using any

electrical device. Before connecting the electrical

supply, confirm that the supply voltage is within the

range stated on the rating label, and see that the

device is securely positioned. Place the unit in a safe

and dry location; it must NOT touch the surrounding.

Follow the safety precautions for chemicals / dangerous

materials.

1. NEVER access dangerous chemicals or other

materials to prevent possible hazard of explosion

and damage.

2. Do not operate the unit without lids or covers to

prevent possible hazards.

3. A temporary conductivity caused by condensation

might occur even though this series is rated

Pollution Degree 2 in accordance with IEC 664.

Environmental Operating Conditions

Ensure the instrument is installed and operated strictly

under the following conditions:

1. Indoor use only

2. < 95% Relative Humidity

3. 75 - 106 kPa

4. Altitude must not exceed 2000 meters

5. 4 - 40 °C operating temperature

6. Pollution degree: 2

7. Mains supply voltage must not fluctuate more than

±10% of the normal voltage

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Avoiding Electrical Shock

Follow the guidelines below to ensure safe operation of

the unit.

The mA700 Essential Power Supply has been designed

to utilize shielded wires thus minimizing any potential

shock hazard to the user. We recommend against the

use of unshielded wires.

To avoid electrical shock:

1. In the event of solution spilling on the instrument,

it must be dried out for at least 2 hours and

restored to NORMAL CONDITION before each

operation.

2. Never connect or disconnect wires loading from the

power jacks when the red indicator light of power

switch is on.

3. WAIT at least 5 seconds after stopping a run

before handling output leads or any connected

apparatus.

4. ALWAYS make sure that your hands, work area,

and instruments are clean and dry before making

any connections or operating the power supply.

5. ONLY connect the power cord to a properly

grounded AC outlet.

Avoiding Damage to the Instrument

1. Do not attempt to operate the device if damage is

suspected.

2. Protect this unit from physical damage, corrosive

agents and extreme temperatures (direct sunlight,

etc.).

3. For proper ventilation and safety concerns, keep at

least 10 cm of space behind the instrument, and at

least 5 cm of space on each side.

4. Use high level of precaution against potential

damage on the unit.

5. Do not operate the unit out of environmental

conditions addressed above.

6. Do not operate the power supplies in high humidity

environments (> 95%), or where condensation may

occur.

7. To avoid condensation after operating the power

supply in a cold room, wrap the unit in a plastic bag

and allow at least 2 hours for the unit to equilibrate

to room temperature before removing the bag and

operating the unit.

8. Prior to applying any cleaning or decontamination

methods other than manufacturer’s

recommendation, users should check with the

manufacturer’s instruction to see if the proposed

method will damage the equipment.

Technical Specifications

Output Voltage/

Inc. 5-300 V / 1 V

Output Current/

Inc. 1-700 mA / 1 mA

Max. Output Watt 150 W

Rated Voltages 100 - 240 Vac, 50 / 60 Hz

200 W: T2.5A / 250 V

Type of Output

1. Voltage or Current with automatic

crossover.

2. When target constant mode is set,

system automatically adjusts the two

other parameters to maximum to allow

constant run (later could be changed by

user).

Program

Multi-Step Up to 6 steps

Editable Program

Function

1. Have typical running conditions

program

2. Manual editable program

Display 2.4” TFT

Control Microprocessor controller

Safety Device

No Load detect

Leakage detect

Over temperature protection

Overload detection

Sudden load change detection (could be

enabled by proper setting)

Shrouded plugs and sockets

Timer

Constant: 1-9999 mins with alarm,

continuous

Program: 1-999 mins with alarm,

continuous

Crossover Yes

Stackable Yes

Automatic

Recovery Yes

IQ/OQ Protocols Yes, optional

Operating

Temperature 4-40 °C

Construction

Material Flame-retardant ABS faceplate

Unit Dimension 215 mm x 335 mm x 104 mm

(W x L x H)

Weight Approx. 2.1 kg

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Installation Instructions

1. Place the unit on a sturdy and level surface in a

safe dry place away from laboratory traffic.

2. Ensure that the power switch is OFF, and then plug

one end of the three-pronged power cord into a

grounded three-prong AC outlet with appropriate

voltage (100 V to 240 V, as indicated on the rating

sticker near the AC cord on the back of the unit)

and plug the other end into the main power socket.

Power Switch

3. Connect the DC output jacks from the

electrophoresis unit; insert the red lead (+) into

the red output jack, and the black lead (-) into

the blue output jack.

4. Power ON the unit by pressing the ON / Off switch

on the back.

Operating Instructions

Control Interface

LED Indicator

Indicates running

conditions

Button Functions

Key

Descriptions

Icon Function

Start

Stop Press to activate or stop the unit.

Pause

Press to temporarily interrupt power to an

operation in progress; resume power after

pausing without resetting the timer.

Page

Toggle Press to go back to previous page.

Enter 1. Press to enter the next page.

2. Press to enable numeric set-up.

Left

Arrow

Press to move cursor left forward between

parameters.

Right

Arrow

Press to move cursor right forward

between parameters.

Arrow

1. Press to move cursor up between

parameters.

2. Press to increase numeric values.

Down

Arrow

1. Press to move cursor down between

parameters.

2. Press to decrease numeric values.

Plugs

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General Operating Instructions

There are 2 modes available: Constant Mode, and

Program Mode.

Constant Mode Setup

Use the Constant Mode for applications that require

only one specific limit: voltage / current / power during

the entire operation of electrophoresis.

1. Use the Up and Down

arrow keys to select

the Constant Mode

button. Use the Enter

key to select.

2. Use the Up or Down

arrow keys to select

any of:

> Constant Voltage (V)

> Constant Current (mA)

> Constant Power (W)

3. Use the Enter key to

select.

4. Use the Up or Down

arrow keys to toggle

between

> Voltage (V)

> Current (mA)

> Power (W)

> Time (minutes)

5. Use the Enter key to

select the specified

parameter to set target value.

6. Use the Up or Down arrow keys to adjust the value

for the selected parameter. Use the Enter key to set

value.

Note: Under Constant Mode, when target value

is set, system automatically adjusts the other two

parameters to maximum to allow constant run. For

example, if constant voltage (V) is set, system will

adjust current (mA) and power (W) to the maximum

value. User could later lower the other two values.

System will hold the value either at the target

constant value or the one which has been reached

first. If the time value is set to “0,” it means infinite,

and the power supply will constantly operate until

the user manually ends the run.

Constant Mode Operation

1. Press the Start

Stop key to start

operation. The LED

is lit, and the screen

will show real-time

parameter values.

When parameter

reaches the set value,

its color will turn

orange.

2. Press the Toggle key to switch the display between

real-time value and set value page.

Note: When a run is completed, operation stops with

an alarm, and COMPLETE is displayed on the screen.

3. Press the Start Stop key to terminate the run.

4. Turn the power off by using the switch on the back

before removing the power leads.

Note: It takes approximately 5 seconds for the unit

to power off.

5. Press the Pause key to temporarily pause the

ongoing run without terminating the timer. The

LED will flash to indicate that the run is paused.

Press the Start Stop key to resume the run.

6. Press the Start Stop key to terminate the run.

To modify any parameters during the operation

(without terminating the timed run), pause the run

by pressing the Pause key.

7. Press the Toggle key to enter the Settings page.

8. Adjust the desired parameter and then press the

Start Stop key to resume the run.

9. To terminate and start a new run during operation,

press the Start Stop key instead of the Pause key.

10. After adjusting the parameters, press the Start

Stop key again to start a new run.

Note: To terminate and start a new run, the timer

will reset and does not take into account the time

before.

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Program Mode Setup

Use the Program Mode to vary levels in voltage

(V), current (mA), and power (W) during specified

time periods for up to 6 Steps, depending upon your

electrophoresis needs. The mA700 Essential Power

Supply will store up to 30 different program files stored

for user’s convenience. Follow the instructions below to

set the Program Mode.

1. Use the Up and Down

arrow keys to select

Program Mode.

Use the Enter key to

select.

2. Use the Up and Down

arrow keys to select

the appropriate file

number, then press

the Enter key to

select.

3. Use the Up or Down

arrow keys to toggle

between

> Voltage (V)

> Current (mA)

> Power (W)

> Time (minutes)

4. Use the Enter key to

select the specified

parameter to set

target value.

5. Use the Enter key

and the Up and Down

arrow keys to set the appropriate values, then

press the Enter key to set. Repeat this step until all

the parameters are set properly. There are 6 steps

available.

Note: If the time value is set to “0” at any stage, it

means stop at previous stage. For example, if Stage

1 is set to 5 minutes and Stage 2 is set to “0,” the

system will only complete Stage 1 and stop.

Program Mode Operation

1. Press the Start Stop

key to start operation.

The LED is lit, and

the screen will show

real-time parameter

values. When

parameters reach the

set value, the color

will turn orange.

2. Press the Toggle key to switch the display between

real-time value and set value page.

Note: When a run is completed, operation stops

with an alarm and COMPLETE is displayed on the

screen.

3. Press the Start Stop key to terminate the run.

4. Turn off the power using the back switch before

removing the Power Leads.

Note: It takes approximately 5 seconds for the unit

to power off.

5. Press the Pause key to temporarily pause the

ongoing run without terminating the timer. The LED

will flash to indicate that the run is paused. Press

the Start Stop key to resume the run.

6. Press the Start Stop key to terminate the run.

To modify any parameters during the operation

(without terminating the timed run), pause the run

by pressing the Pause key.

7. Press the Toggle key to enter the Settings page.

8. Adjust the parameter and then press the Start Stop

key to resume the run.

9. To terminate and start a new run during operation,

press the Start Stop key instead of the Pause key.

10. After adjusting the desired parameters, press the

Start Stop key again to start a new run.

Note: If the run has been terminated, and a new

one started, the timer will reset and does not take

into account the time before.

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Replacing the Fuse

1. Turn off the main power switch at the rear of

Power Supply and detach the power cord.

2. Open the fuse compartment located at the Main

Power Socket by inserting a small flat blade

screwdriver into the slot below the ON/OFF switch.

Turn the screwdriver to gently pry open the fuse

compartment.

Note: The fuse compartment will not open with the

power cord in place.

3. Pull the fuse holder out of the compartment and

inspect the fuse. If the fuse is burned or there is a

break in the fuse element, replace the fuse with an

identical type of fuse (T2.5AH250V) as provided in

the fuse holder

(see figure right).

4. Place the fuse holder back into the compartment.

5. Snap the cover closed.

Troubleshooting and Maintenance

Many operating problems may be solved by carefully reading and following the instructions in this manual

accordingly. Should these suggestions not resolve the problem, visit the Technical Service at

SigmaAldrich.com/techservice.

Problem Possible Causes Solution

No display or lights.

No AC power. Check if power supply is plugged in,

or if AC power source has problem.

AC power cord is not connected. Check the AC power cord connections

at both ends. Use the correct cords.

The fuse has blown. Replace the fuse.

Fuse broken repeatedly. Hardware failure. Contact our Technical Service Department.

Operation stops and

the screen displays

PLC ERROR.

Communication wires on circuit

board have loosened or broke.

We recommend that you send the machine

back to your local distributor or our Technical

Service Department for maintenance.

Operation stops with alarm.

The screen displays

NO LOAD.

Electrophoresis leads are not

connected to the power supply

or to the electrophoresis unit(s),

or there is a broken circuit in

the electrophoresis cell.

Check the connections to the power supply and

on your electrophoresis cell to make sure the

connection is intact; check condition of wires

in electrophoresis unit. Close the circuit by

reconnecting the cables. Press

START / STOP to restart the run.

High resistance due to tape

left on a pre-cast gel, incorrect

buffer concentration, or

incorrect buffer volumes in the

electrophoresis cell

Make sure the tape is removed from the

pre-cast gel, buffers are prepared correctly, and

the recommended volume of buffer is added to

the electrophoresis unit.

High voltage application is set to

run on a very low current.

DISABLE the No Load alarm on the Display

Screen.

Operation stops with alarm.

Display shows:

LOAD CHANGED.

Bad connections for terminal

connectors or damaged wires or

damaged platinum wires.

Check all the connections to terminators,

cables, wires, and gel tanks.

Fuse in Use

Extra Fuse

Fuse Holder

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Problem Possible Causes Solution

Operation stops with alarm.

Display shows:

OVER VOLTAGE.

Circuit is interrupted.

Verify that the running buffer is correct.

Verify that all cables are attached correctly.

Turn the Power switch off and on again; restart

application.

If you cannot restart the instrument, turn off

the power, disconnect the power cord from the

outlet, and contact Technical Service.

Operation stops with alarm.

Display shows:

OVER CURRENT.

Circuit is interrupted.

Verify that the running buffer is correct.

Verify that all cables are attached correctly.

Turn the Power switch off and on again; restart

application.

If you cannot restart the instrument, turn off

the power, disconnect the power cord from the

outlet, and contact Technical Service.

Operation stops with alarm.

Display shows: LEAKAGE.

Ground leak detected during

run.

Check the electrophoresis system for improper

grounding. Restart the power supply by turning

the Power switch off and on.

Operation stops with alarm.

Display shows:

OVER TEMPERATURE.

Power supply is overheating.

Turn off power supply. Check for sufficient

airflow around the power supply fan. After

cooling down, restart the power supply by

turning the Power switch to the on position.

If you cannot restart the instrument, turn off

the power, disconnect the power cord from the

outlet, and contact Technical Service.

Warning message displays

with 5-second beeping

sound. Display shows:

POWER RECOVERY.

The power that had been cut

has now recovered.

User does not need to take extra action. The

warning sign and beep sound would only last

for 5 second; after that, the machine will

continue running the unfinished project.

The sign indicates that the machine has

been interrupted by sudden power off. Press

the ENTER key to clear the sign.

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The life science business of Merck operates

as MilliporeSigma in the U.S. and Canada.

Merck, Millipore and Sigma-Aldrich are trademarks of Merck KGaA, Darmstadt, Germany or its

affiliates. All other trademarks are the property of their respective owners. Detailed information

on trademarks is available via publicly accessible resources.

Product Ordering Information

The mA700 can be purchased with different power

supply cords to accomodate a variety of locations.

Purchase products online at SigmaAldrich.com/products.

Model Description Catalog

Number

mA700

Essential

Power

Supply