BRIGHT M3500 MICROTOME Guide

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 1
Operating, Maintenance and
Spare Parts Manual for the
Bright
M3500 MICROTOME
Serial Number: ………………………

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013
2
CONTENTS
OPERATING, MAINTENANCE AND ..............................................................1
SPARE PARTS MANUAL FOR THE ..............................................................1
BRIGHT ...........................................................................................................1
M3500 MICROTOME.......................................................................................1
Serial Number: ……………………….................................................................................................1
CONTENTS ........................................................................................... 2,3 & 4
SAFETY AND GENERAL INFORMATION .....................................................6
Consumer Protection..........................................................................................................................6
ELECTRICAL.................................................................................................................................... 6
MICROTOME KNIVES/BLADES ......................................................................................................6
OPERATION..........................................................................................................................................7
ACCESSORIES......................................................................................................................................7
PRODUCT SAFETY SUGGESTIONS ................................................................................................7
DECONTAMINATION CERTIFICATES ..........................................................................................7
WARRANTY ..........................................................................................................................................8
1. INTRODUCTION .......................................................................................9
1.1 Receipt and Unpacking ......................................................................................................9
1.1.1 Receipt .............................................................................................................................9
1.1.2 Unpacking .......................................................................................................................9
1.1.3 Lifting ..............................................................................................................................9
1.2 ASSEMBLY AND INSTALLATION ........................................................9
1.2 Electrical connections......................................................................................................... 9
1.2.1 Electrical requirements ..................................................................................................9
1.2.2 Electrical Safety ..............................................................................................................9
1.2.3 Switching on ....................................................................................................................9
2. OPERATING INSTRUCTIONS..................................................................9
2.1 Front Control Panel..........................................................................................................10
2.2 REAR FUSE PANEL ............................................................................10
2.1.1 Mains Switch.................................................................................................................10
2.1.2 Fuse ................................................................................................................................10
2.3 PRE-USE CHECKS..............................................................................11
2.4 MICROTOME CONTROLS...................................................................11
2.4.1 Specimen cassette.......................................................................................................... 11
2.4.2 Knife Holder..................................................................................................................11
2.4.3 Disposable Blades ......................................................................................................... 11
2.4.4 Rewind Control (Manual)............................................................................................ 12
2.4.5 Section Thickness Control ........................................................................................... 12

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013
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2.5 MOTORISED ADVANCE/REWIND (WHERE FITTED)........................12
2.5.1 General ..........................................................................................................................12
2.5.2 Motorised Specimen Cassette Rewind ........................................................................ 12
2.5.3 Motorised Specimen Cassette Advance ......................................................................12
2.6 OTHER FEATURES .............................................................................12
2.6.1 Debris Trays.................................................................................................................. 12
2.6.2 Visor...............................................................................................................................13
2.6.3 Hand wheel.................................................................................................................... 13
2.7 CUTTING AND COLLECTING SECTIONS..........................................13
2.7.1 Set Up.............................................................................................................................13
2.7.2 Wax Cassette Set Up..................................................................................................... 13
2.7.3 Trimming.......................................................................................................................13
2.7.4 Section Cutting.............................................................................................................. 13
3. GENERAL MAINTENANCE....................................................................14
3.1 Daily Care..........................................................................................................................14
3.2 Microtome Knives and Blades......................................................................................... 14
3.2.1 Conventional Knives..................................................................................................... 15
3.2.2 Disposable Blades ......................................................................................................... 15
3.3 SERVICING AND REPAIRS.................................................................15
3.3.1 Routine Servicing..........................................................................................................15
3.3.2 Repairs........................................................................................................................... 15
3.4 DECONTAMINATION ..........................................................................16
3.4.1 Disinfectant ...................................................................................................................16
4. SECTIONING...........................................................................................16
A PROBLEM SOLVING QUIDE.......................................................................................................16
4.1 CHECK LIST ........................................................................................16
4.1.1 Specimen........................................................................................................................ 16
4.1.2 Knife...............................................................................................................................16
4.1.3 General Factors.............................................................................................................16
4.2 THICK / THIN SECTIONS ....................................................................17
INTERMITTENT FAILURE TO CUT..............................................................................................17
4.3 SECTIONS CRUMBLING OR NOT FORMING ....................................17
4.4 EXCESSIVE COMPRESSION OF SECTIONS.....................................17
4.5 UNEVEN THICKNESS ACROSS SECTION ........................................17
4.6 VERTICAL SCORE MARKS ON SECTIONS.......................................17
4.7 SECTIONS FAIL TO FLATTEN AND PASS DOWN KNIFE ................18
5.0 MISCELLANEOUS SERVICING INSTRUCTIONS ..............................18
5.1 Advance Pawl Replacement............................................................................................. 18
5.2 Pawl Release Lever (Advance).........................................................................................18

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013
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5.3 Calibration ........................................................................................................................18
9. SPECIFICATIONS.....................................................................................21
Accessories ........................................................................................................................................22
MATERIAL SAFETY DATA SHEET .............................................................23
Name: CRYO-M-BED.............................................................................................................. 23
MATERIAL SAFETY DATA SHEET .............................................................24
Name: CRYOSPRAY ...............................................................................................................24
MATERIAL SAFETY DATA SHEET .............................................................25
Name: Low temperature Grease .............................................................................................25
Knives / Blades.................................................................................................................................. 26
DECONTAMINATION CERTIFICATE...........................................................27
DECONTAMINATION PROCEDURES .........................................................28
Microtomes............................................................................................................................................28
Microtome Knives ................................................................................................................................28
QUALITY SURVEY REPORT........................................................................29
Annexe:
Drawing 212-040 M3500 Microtome Assembly 1 of 3 30, 31 & 32
Part List 212-040-PL 33 & 34
Drawing 51937 Cutting Arm Assembly 35
Part List 51937-PL 36
Drawing 212-041 Microtome Motor Rewind Sub-Assembly 37
Part List 212-041-PL 38
Drawing 212-046 CQRS Non-Orientating Assembly 39
PartList212-046-PL 40
Drawing 212-047 CQRS Orientating Head Assembly M3500 41
Part List 212-047-PL 42
Drawing 212-051 Pawl Release Assembly 43
Part List 212-051-PL 44
Drawing 212-054 M3500 Knife Block Retract Model Sub-Assembly 45
Part List 212-054-PL 46 & 47
Drawing 212-061 M3500 Input Drive Shaft (Hand wheel) Assembly 48
Part List 212-061-PL 49
WiringDiagram 50

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 5
Power IndicatorSection Counter Reset
Section Counter
Visor
Thickness
Selector Knob
Locking
L
eve
r
Rear
Cove
r
Cutting
Handwheel
RH Advance/Rewind
Sw
i
tc
h
50230 Standard
Knif
e
RH Knife
Gua
r
d
Knife Guard
H
e
i
g
h
t
Kn
ob
Debris
Tray
Knife
Clamps
Cassette Release
L
eve
r
Manual
A
dv/
R
wd
Kn
ob

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 6
SAFETY AND GENERAL INFORMATION
Consumer Protection
The Consumer Protection Act 1987 Part 1. refers to Product Liability. This legislation was
issued as a direct result of an EC Directive to all member states and has been in force with
effect from 1 March 1988.
The Bright Instrument Company Limited, ever mindful of the need to ensure that their
products are not subject to misuse and/or incorrect handling, have made it their aim to
communicate any possible dangers to their customers.
Whilst the Bright Instrument Company Limited markets products manufactured to the highest
safety standards, it is in the interest of the purchaser that he is aware of the resultant dangers
of misuse and/or incorrect handling of these products.
Your attention is therefore drawn to the following precautions:
ELECTRICAL
a. Warning - A warning notice is fixed to the instrument (where applicable) stating that
it should be disconnected from the mains supply before removing the panels. This
warning should be strictly observed.
b. Fuses – Fuse ratings are clearly indicated on all fuse panels adjacent to the fuse
holder. If and when replacement is necessary, the correct fuse rating and type must
be adhered to.
c. Earthing (Grounding) – A protective earth terminal is fitted and must be used in all
two-wire installations.
MICROTOME KNIVES/BLADES
The microtome knife/blade can be a hazard in the laboratory. Personnel should be made
aware of the dangers and observe the following warnings:
a. DO NOT leave the microtome unattended with an exposed knife/blade in position.
Remove the knife/blade, or cover with the guards or visor provided.
b. DO NOT leave unboxed knives/blades lying around. Place knives/blades that are not
in use in their boxes or packets.
c. DO NOT carry knives/blades unless secure in the box or packet provided.
d. DO NOT clean the knife/blade along its length. Wipe the knife/blade from the back
edge to the cutting edge.
e. REMEMBER that even used knives and blades are dangerous. The are still sharp
and may have been used to cut potentially infectious specimens.
f. DISPOSE of used knives and blades with the same care as other sharp objects. On
no account should used knives or blades be placed in waste bins.

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 7
OPERATION
When placing specimen cassettes in the microtome, when orientating, manipulating or in any
way placing fingers in a position above the knife/blade edge – ensure that the hand wheel is
locked and knife guards are in position.
ACCESSORIES
The instrument has the following accessories as standard:
Low temperature grease: (please refer to the material safety data information, towards the
back of this instructions manual, for further information).
Long series ball driver tool, to remove / fit rear cover
2 pin tool, to remove / fit the hand wheel and visor
Allen keys, fuse and brush
PRODUCT SAFETY SUGGESTIONS
All Bright Instrument Company Limited personnel are encouraged to make suggestions
regarding product safety. We also welcome such suggestions from our customers. They may
be submitted by completing the appropriate (Safety) section of the Quality Survey Record
Form supplied with all Bright instruments, or alternatively, by letter, telephone, fax or email. All
communications should be direct to our Quality Assurance Department and will be
acknowledged.
DECONTAMINATION CERTIFICATES
IMPORTANT
If the instrument or any part of it is to be returned to Bright Instrument Company Limited, a
decontamination certificate must accompany it, and please note the following:
1. If the instrument or any part of it has been exposed to or been in contact with potential
pathogenic or radioactive material, it is essential that it be decontaminated.
2. Set procedures are laid down by the Health and Safety Executive for decontamination.
The “Howie code” and the “Code of Practise for the Prevention of Infection in Clinical
Laboratories and Post-mortem Rooms”, ISBN 0 11 320464 7) explain the correct
procedures to be used. For the avoidance of doubt, we ask that a completed
decontamination certificate should accompany instruments or parts returned to us. A copy
of this can be found at the back of this instruction manual and we suggest you use a
photocopy of this when returning parts. Alternatively we would be pleased to either post or
fax you another copy should you require.
3. Should the instrument or any part of it be received in a condition that Bright Instrument
Company Limited, consider to be a potential biological hazard, the instrument or part will
be returned unrepaired at the expense of the customer.
4. Customer’s declarations must indicate that the package contains ‘British Returned
Goods’. Failure to do so will involve customs duty payable by us, which will be invoiced to
the sender.

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 8
WARRANTY
The Warranty relating to the instrument is outlined in our Terms and Conditions of Sale
paragraph 6.
i) The Seller‘s products are carefully inspected and submitted to its standard tests.
II) The Seller warrants all its products to be free from defects in workmanship and
materials under normal conditions of use and service provided always:
a. that if any of the goods so manufactured is alleged to be defective in workmanship
and material and is returned carriage paid, and protected against damage in transit to
the Seller’s works at Huntingdon, England within 12 months from the date of despatch
and if after examination by the Seller that goods or part of them are found to be so
defective then the Seller will repair or replace them free of charge and will return them
to the Buyer , carriage paid.
b. where any part of the goods manufactured by the Seller is repaired or replaced under
the terms of the foregoing warranty, such warranty shall thereafter be limited to a
period of six months from the date when the goods shall have been re-delivered to
the Buyer.
c. this warranty does not apply to any defects caused by wear and tear, incorrect
installation abnormal conditions of working, accident, misuse or neglect.
d. that save as in this clause herein before expressed, the Seller shall not be under any
liability for negligence or otherwise in respect of defects in goods delivered or for any
injury, damage or loss resulting from such defects and the Seller’s liability under this
clause shall be in lieu of any warranty or condition implied by law as to the quality or
fitness for any particular purpose of such goods.
e. this warranty is expressly in lieu of all other warranties, guarantees or liabilities
expressed or implied by any of the Seller’s Representatives or Agents.
Please see our separate Product Warranty sheet for deliveries to the mainland UK.
WARNING
Before proceeding to Operating Instructions, ensure you are familiar with the contents
of the pages marked ‘Safety Information’. This instrument must only be used by
competent persons.

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 9
1. INTRODUCTION
1.1 Receipt and Unpacking
This instrument received a final test and inspection prior to despatch from the factory. The
following instructions are given for the re-assembly of the instrument, adjustments and its
correct use. If the instrument is received before preparations for installation are completed, it
should be stored in a clean, dry place and not exposed to dirty or damp conditions.
1.1.1 Receipt
Immediately upon receipt of the instrument, make a careful examination for evidence of
damage encountered in transit. If any damage is found or suspected, notify both the carrier
and Bright Instrument Company Limited.
1.1.2 Unpacking
All packing must be carefully removed and parts checked against the enclosed packing list. If
any damage or discrepancy is noted, please inform our agent/distributor, or Bright Instrument
Company Limited, direct, immediately.
1.1.3 Lifting
The instrument has been designed with two main lifting points as hand holds at the front and
rear of the instrument. The instrument weight less than 25Kgs, but care should be taken when
lifting by following manual handling guide lines. If in doubt contact your Health & Safety
Officer.
1.2 ASSEMBLY AND INSTALLATION
The microtome is delivered virtually ready for use with only the accessories required for use in
need of fitting.
1.2 Electrical connections
1.2.1 Electrical requirements
The supply cord of the instrument should be connected to any ordinary electrical outlet (max
13 amps). Check the voltage stamped on the nameplate, located on the underside of the
instrument, with your supply:
The connections are:
Brown - Positive [live]
Blue - Negative [neutral]
Yellow/Green - Earth [ground]
1.2.2 Electrical Safety
Where earth connections are taken through connectors, then the connector must be rated to
take the maximum fault current. The machine should be disconnected before such connectors
are separated for servicing purposes.
1.2.3 Switching on
Switch on by using the Mains Power switch located on the rear fuse panel. This is applicable
only to Motorised Advance/Rewind models.
For instruments with a section counter only, this is operated by 2 AA batteries located on the
rear panel at the bottom of the plinth.
2. OPERATING INSTRUCTIONS
On receipt of your new Bright instrument, please refer to section 1.1 (Receipt and Unpacking)
and section 1.2 (Assembly and Installation).

M3500 Issue 2 - Feb 2006
Last reviewed: Oct 2010 / Next review Oct 2013 10
As part of its policy of continual improvement, Bright Instrument Company Limited, reserves
the right to incorporate changes, or make additions to, without prior notice. There may,
therefore, be minor details differences between the information in this manual and your
instrument. These differences will not affect the safety and use of the instrument.
2.1 Front Control Panel
This panel houses the section counter, the re-set button to zero the counter and a Mains
Power LED for indicating purposes.
2.2 REAR FUSE PANEL
2.1.1 Mains Switch
The mains power switch provides power to the motorised advance/rewind control only.
2.1.2 Fuse
The single fuse is for the Main Power only.
Main - All circuits
If the fuse blows, replace it with a new one of exactly the same rating. In the event of
repeated fuse blowing, consult a qualified electrical engineer.
On NO account must the fuse be replaced with anything other than a correctly rated
substitute.