MMM Group CO2Cell 190 Standard User manual

Instructions for use
CO2 INCUBATOR
CO2Cell 190 Standard
CO2Cell 190D Standard
(with decontamination 160 °C)
CO2CELL Standard 190_np_en 1301_mmm_V1.02

CONTENTS:
24. 1. 2013
1SAFETY ......................................................................................................................................................3
1.1 LEGAL CONSIDERATIONS ............................................................................................................................ 3
1.2 INTENDED USE ......................................................................................................................................... 3
1.3 SYMBOLS USED IN THIS MANUAL.................................................................................................................. 3
1.4 GENERAL SAFETY INSTRUCTIONS................................................................................................................. 3
2DESCRIPTION ............................................................................................................................................. 3
2.1 CONSTRUCTION ....................................................................................................................................... 4
2.2 DOORS .................................................................................................................................................. 4
2.3 CONTROL SYSTEM.................................................................................................................................... 4
2.4 CO2SENSOR ........................................................................................................................................... 4
2.5 DIAGRAMS .............................................................................................................................................. 4
2.5.1 FRONT AND INNER VIEWS ...................................................................................................................... 4
2.6 REAR CONNECTIONS ................................................................................................................................. 4
3DELIVERY, TRANSPORTATION AND STORAGE...................................................................................... 5
3.1 UNPACKING............................................................................................................................................. 5
3.2 LIFTING AND MOVING ................................................................................................................................ 5
3.3 STORAGE ............................................................................................................................................... 5
4INSTALLATION............................................................................................................................................5
4.1 LOCATION AND AMBIENT CONDITIONS............................................................................................................ 5
4.2 SHELVES AND USABLE SPACE ..................................................................................................................... 6
4.3 WATER TRAY........................................................................................................................................... 6
4.4 GAS CONNECTION .................................................................................................................................... 6
4.5 ELECTRICAL CONNECTION .......................................................................................................................... 6
5GETTING STARTED....................................................................................................................................7
5.1 START UP .............................................................................................................................................. 7
5.2 FACTORY PRESETS ................................................................................................................................... 7
5.3 MAIN DISPLAY.......................................................................................................................................... 7
5.4 CONTROL MENUMAP ................................................................................................................................. 7
5.5 CHANGING TEMPERATURE SET POINT ........................................................................................................... 8
5.6 CHANGING CO2SET POINT ......................................................................................................................... 8
5.7 HIGH AND LOW TEMPERATURE ALARMS ........................................................................................................ 8
5.8 CHANGING HIGH TEMPERATURE ALARM ......................................................................................................... 8
5.9 CHANGING LOW TEMPERATURE ALARM.......................................................................................................... 8
5.10 HIGH AND LOW CO2ALARMS ....................................................................................................................... 9
5.11 CHANGING HIGH CO2ALARM ....................................................................................................................... 9
5.12 CHANGING LOW CO2 ALARM ........................................................................................................................ 9
5.13 DOOR OPEN ALARM..................................................................................................................................10
5.14 CHANGING THE DOOR OPEN ALARM.........................................................................................................10
6HIGH TEMPERATURE 160 °C DECONTAMINATION...............................................................................10
6.1 ABOUT THE DECONTAMINATION CYCLE.................................................................................................................................10
6.2 PREPARATION.........................................................................................................................................................10
6.3 STARTING THE CYCLE...........................................................................................................................................................11
6.4 CYCLE TIME........................................................................................................................................................................11
6.5 COOLING PHASE..............................................................................................................................................................11
7OPTIONS .....................................................................................................................................................11

7.1 BMS VOLT FREE ALARM RELAY..........................................................................................................................................11
7.2 MULTI-DOORS..........................................................................................................................................................11
8ACCESSORIES..................................................................................................................................12
9CLEANING..........................................................................................................................................12
9.1 OUTER SURFACES....................................................................................................................................12
9.2 INNER SURFACES....................................................................................................................................12
9.3 OXIDATION AT HIGH TEMPERATURE..............................................................................................................12
10 SERVICE........................................................................................................................................12
10.1 WARRANTY..................................................................................................................12
10.2 PREVENTATIVE MAINTENANCE.....................................................................................................................12
10.3
RETURNING AUNIT...................................................................................................................................12
11 DISPOSAL............................................................................................................................................13
12 TECHNICAL INFORMATION......................................................................................................................13
12.1 TECHNICAL DATA......................................................................................................................................13
12.2 DIMENSIONAL DRAWINGS...........................................................................................................................13

Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 3
1 SAFETY
1.1 LEGAL CONSIDERATIONS
It is important that you read this manual
completely and observe all instructions.
Failure to follow these instructions may result
in personal injury, equipment damage and
incorrect performance of the incubator.
This manual contains information for installing,
operation and maintenance of the incubator. It cannot cover
all possible uses and applications. Please contact your
dealer or MMM directly if you have a special application
or the manual does not describe your problem in enough
detail.
The contents of this manual and the incubator are subject to
change without notice.
1.2 INTENDED USE
MMM CO2 Cell Standard incubators are intended for
mammalian cell cultivation under typical conditions of
37.0 °C. The pH of the cell media can be controlled by
altering the CO2concentration within the incubator.
Other applications are not approved by MMM. Please
contact your dealer or MMM directly, for advice if you intend
to use this incubator for other applications.
1.3 SYMBOLS USED IN THIS MANUAL
The following symbols are used throughout this manual:
Risk of injury Electrical
hazard
Hot surface Lifting
hazard
Stability
hazard
Compressed
gas hazard
Asphyxiation
hazard
Mandatory
regulation
Read
operating
manual
Lift with
several
persons
Disconnect
from
electrical
supply
Important
information
for correct
operation
1.4 GENERAL SAFETY INSTRUCTIONS
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laboratory personnel who are familiar with all precautions
and standard safety practices for working in a laboratory
environment.
These safety instructions are intended to be used alongside
any local safety guidelines, rules and regulations. If in
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organisation.
Only skilled electricians or engineers authorised by MMM
can perform repairs or maintenance on this incubator. Only
original spare parts from MMM can be used. There are no
user serviceable parts within the incubator.
MMM takes no responsibility for 3rd party accessories or
equipment used with this incubator.
DANGER
Electrical hazard
Danger of death
Do not remove any panels from incubator.
Do not allow the incubator to become wet.
HOT
SURFACES
Danger of burning
Risk of injury
The glass door and inner chamber reach
200 °C during sterilisation.
Do not force the door open during
sterilisation.
STABILITY
HAZARD
Stability hazard
Risk of injury
Damage to unit
Do not place the unit too close to the edge
of a bench.
Do not lean on the door when open.
LIFTING
HAZARD
Lifting hazard
Risk of injury
Damage to unit
Lift with 2 people
95 kg
Observe safe handling regulations
2 DESCRIPTION
MMM CO2&HOO6WDQGDUGLQFXEDWRUVDUH¿WWHGZLWKDGLJLWDO
controller, ensuring precise control of temperature and CO2
levels.
Accurate and stable heating throughout the entire chamber
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heating element. In addition a separate independently
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door is free from condensation.
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convection of the air within the chamber, ensuring even
temperature and gas distribution throughout the chamber.
MMM CO2Cell Standard 190 D incubatorhas extra heaters
that allow high temperature 160 °C decontamination of the
incubator chamber.

Instructions for use
4CO2CELL Standard 190_np_en 1301_mmm_V1.02
2.1 CONSTRUCTION
The inner chamber is made from 304 grade stainless steel.
It is deep drawn from one piece with no welds and is
electro-polished, meaning that the inner surfaces are
smooth and easily cleaned.
The removable shelves, racking and water tray are also
made from stainless steel.
The outer housing is made from stainless steel and powder
coated with anti-bacterial biocide paint.
2.2 DOORS
The outer door has a heater on its inner face. This door is
an integral part of the temperature control system and must
be closed for the incubator to function normally.
The door is hinged on the right hand side. Left hand opening
is only available as an option at the point of ordering, retro-
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When this door is open, the CO2valve is closed and
heater power is reduced to prevent over shoot of operating
conditions. The door is linked to the controller alarm system.
DSection 5.13.
An inner glass door seals against a thermoplastic elastomer
gasket. This allows viewing of the samples within the
chamber without disturbing the temperature or atmosphere
of the chamber.
The incubator can be optionally equipped with a multi-door.
These multi-doors improve temperature and CO2recovery
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DSection 7.2.
2.3 CONTROL SYSTEM
The digital controller allows the user to easily see operating
conditions and to alter settings via push buttons.
Software controlled PID loops constantly monitor
temperature and CO2values, making automatic adjustments
to keep the chamber atmosphere within limits.
2.4 CO2SENSOR
CO2is measured and controlled using a highly accurate
infra-red sensor. This sensor remains practically drift free
between calibration periods. The sensor is not mounted
within the chamber; instead a small pump passes the
chamber atmosphere through the sensor. The CO2sensor
does not have to be removed during high temperature
decontamination.
2.5 DIAGRAMS
2.5.1 FRONT AND INNER VIEWS
2.6 REAR CONNECTIONS

Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 5
3 DELIVERY,
TRANSPORTATION
AND STORAGE
3.1 UNPACKING
Inspect the packaging for signs of transportation damage.
Carefully undo the top of the cardboard packaging and
gently slide the packaging over the incubator.
Inspect the incubator and inform the carrier immediately if
there are any signs of transportation damage.
Remove any packaging material from within the chamber.
Remove any protective coverings from the surfaces of the
inner chamber.
Remove the Instructions for use and accessory kit from
within the chamber. Keep these safe.
Please check that the accessory pack contains the following
items. Contact your dealer with details of
any shortages.
Standard 190 / 190D accessory pack Qty
Stainless steel perforated shelves 4
Shelf racking (left and right hand) 1 set
Racking support bar 1
Stainless steel water tray 1
Service port bungs 2
Instructions for use 1
3.5 meter length of CO2tubing with HEPA
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1
Hose clips 2
Test report 1
IEC mains power lead 1
3.2 LIFTING AND MOVING
Model CO2cell standard 190 weighs 95 Kg. Standard 190D
weighs 104 Kg.
Please refer to Manual Handling Policy of your organisation
before attempting to lift the incubators.
Risk of unit sliding or tipping.
Risk of injury by lifting heavy loads.
Risk of damage to the unit.
Always lift using the handles on the outside
of the incubator.
Lift with 4 people.
Do not lift by using the door.
3.3 STORAGE
If the incubator has been in a cold location for a
period then it needs to acclimatise to room
temperature.
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before start up. This will allow any condensation that may
have formed to dry. It will also stop the incubator from
overshooting temperature set point.
4 INSTALLATION
4.1 LOCATION AND AMBIENT
CONDITIONS
The incubator can be placed on a table or worktop.
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enough to carry the weight of the incubator.
MMM do not recommend placing the incubator directly on
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Wheeled platforms are available to lift the incubator off the
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optional stacking kit. DSection 8.
Correct location of an incubator is very important. The
incubator is designed to work in a typical laboratory
environment.
For good performance the ambient conditions should be at
least 6 °C below the working temperature.
E.g. for a set point of 37.0 °C the ambient temperature must
not exceed 31.0 °C.
If the difference between the set point and ambient
temperatures is less than 6.0 °C then the set point can be
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Our performance data was produced at the ideal ambient
condition of 25.0 °C. For best performance the ambient
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Maximum ambient humidity is 70 % rH, non-condensing.
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exceeded.
A poorly located incubator can experience temperature
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cultures. These guidelines will ensure best performance:
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xIt should be in a well ventilated and dry area, but not
sited directly in the path of ventilation ducts.
xIt should be free from vibration.
xIt should be kept away from heat sources e.g. radiators,
heating pipes, refrigeration plant.
xIt should not be in direct sunlight.
xTo avoid contamination it should not be placed directly
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xThere should not be big swings in ambient temperature.
Leave a clearance of 50 mm from each side and 100 m from
the rear.
Level the incubator by adjusting the four feet. Place a small
spirit level on the centre position of the middle shelf as a
guide.

When it is level, secure the feet by tightening the lock nuts
with a 10 mm spanner.
4.2 SHELVES AND USABLE SPACE
Each incubator is supplied with 4 perforated stainless steel
shelves. The shelves locate into removable stainless steel
racking. The shelves incorporate an anti-tilt mechanism,
stopping the shelves from being pulled out too far.
Each shelf measures 690 mm wide by 390 mm deep. The
minimum spacing between shelves is 80 mm. There are 8
possible positions for the shelves.
The shelves and racking can be left in place during
decontamination.
The usable space within the incubator is the volume
in which the conditions are within our published
data.
A, B, C = Internal dimensions
a = distance from left and right wall = 70 mm
b = distance from top = 70 mm
c = distance from front and back wall = 70 mm
d = distance from base = 70 mm
For practical purposes this is the perforated area on each
shelf.
xDo not place any samples outside of this space.
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xDo not use the top shelf for very deep samples.
4.3 WATER TRAY
A stainless steel water tray is supplied which allows the
incubator to provide 90 % rH.
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the side racking.
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water.
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week.
Any spillages within the chamber should be removed and
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cause condensation to form.
Under no circumstances should any equipment be placed in
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Remove the water tray before moving the incubator.
Instructions for use
6CO2CELL Standard 190_np_en 1301_mmm_V1.02
Empty the water tray if the incubator is switched off,
otherwise condensation will occur.
4.4 GAS CONNECTION
Risk of injury. Compressed gas.
Risk of damage to unit.
Observe relevant regulations for
handling CO2
Risk of asphyxiation. Site must have
adequate ventilation.
Risk of CO2poisoning. Site must have
adequate ventilation.
The incubator is designed for use with CO2of
99.5 % purity. Input pressure greater than 1.0 bar
(15 psi) will damage the incubator so a pressure
reducing valve (PRV) must be used.
The CO2inlet to the incubator is via a 4 mm barbed push
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can be supplied by MMM. DSection 8.
For correct connection of CO2, follow these steps in order:
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supply.
2) Fully close the PRV.
3) Open the main valve on the gas bottle or central supply.
4) Adjust the PRV pressure of the gas supply to between
0.3 bar and 0.7 bar (4.5 to 10 psi).
5) Close the main valve on the gas bottle / central supply.
6) Connect the other end of the tubing to the incubator CO2
inlet. Secure with the supplied clip.
Repeat these steps whenever you change the CO2bottle or
supply.
Turn off the main valve and disconnect from the CO2supply
if the incubator is to be unused for extended periods.
4.5 ELECTRICAL CONNECTION
Risk of injury.
Risk of damage to unit. Observe correct
mains supply voltage.
Risk of electrical shock. Do not connect
with wet hands.
No user serviceable parts. Do not
remove service panels.
Disconnect unit in an emergency.
Before connecting the incubator, check that the electrical
supply corresponds with the values on the incubator data
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The incubator is designed to operate from a single phase
electrical supply of: 230 V AC ±10 % 50/60 Hz.
This incubator MUST be connected to a protective earthed
supply.

Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 7
Further details can be found in the technical data.
DSection 12.1
Plug the supplied power lead into the IEC connector on the
rear of the incubator.
Plug the other end of the IEC lead into the electrical supply.
In an emergency you may need to isolate the
incubator from the electrical supply by disconnecting
the mains supply plug. Make sure that the mains
supply plug is easily accessible.
5 GETTING STARTED
5.1 START UP
Turn the incubator on using the power switch on the rear
panel.The incubator will power up and the digital displays
will show the current temperature and CO2level inside the
chamber.
If an alarm sounds on start up, press
Ö
If an alarm sounds on start up, press to silence it.
to
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to show which system is in an alarm state (Temperature,
CO2or both).
5.2 FACTORY PRESETS
The following values are preset to allow you to work straight
away:
Parameter Parameter Value
63WSet Point, Temperature 37.0 °C
63FSet Point, CO25.0 %
+,WHigh temperature
alarm
+1.0 °C
relative to set point
/2WLow temperature alarm -1.0 °C
relative to set point
+,FHigh CO2alarm +1.0 °C
relative to set point
/2FLow CO2alarm -1.0 °C
relative to set point
GRGDoor open alarm delay
timer
30 seconds
5.3 MAIN DISPLAY
The main displaysshow the current temperature and CO2
conditions inside the chamber. If one of the displays is
ÀDVKLQJDQDODUPFRQGLWLRQH[LVWVDSection 5.6 and
Section 5.9 for alarms.
If an alarm sounds on start up, press
Ö
If an alarm sounds on start up, press to silence it.
to
silence it.
The buttons require a 1 second press before any change
happens. This is to guard against accidental changes.
When using the
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
buttons, a long press will
result in a faster change of value on the display.
The controls menu map is shown on the next page.
5.4 CONTROLS MENUMAP
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second) then each subsequent press
moves on one step.
63W
63F
+,W
/2W
+,F
/2F
GRG
Ö
Actual chamber temperature HeaterLED Actual chamber CO2 %
Increase displayed value CO2 injection LED
Menu button / Silence alarm Enter button
Decrease displayed value
Press (for 1 second) to access
+,F
63F
$/
G(&RQ
+,W
/2W
/2F
63W
GRG
HQ*

Instructions for use
8 CO2CELL Standard 190_np_en 1301_mmm_V1.02
5.5 CHANGING TEMPERATURE SET
POINT
To change the chamber temperature:
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press the
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
button or the
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
button to change the
value in the right display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UP
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
Minimum set point is limited to 0.0 °C.
Maximum set point is limited to 60.0 °C.
5.6 CHANGING CO2SET POINT
To change the chamber CO2level:
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left
display and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second).63Fwill show in the left
display and a value .in the right display.
Press the
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
button or the
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
button to change the
value in the right display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UP
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
Minimum set point is limited to 0.0 %
Maximum set point is limited to 20.0 %.
5.7 HIGH AND LOW TEMPERATURE
ALARMS
The incubator has high and low temperature alarm system
as standard. When the chamber temperature triggers the
DODUPWKH7HPSHUDWXUHGLVSOD\ZLOOÀDVKDQGDEX]]HUZLOO
sound.
The high and low alarm limits are relative to the chamber
set point which means that they do not need adjusting
when you change the chamber temperature. The over and
under temperature alarms will “follow” the changes in your
chamber temperature.
For example:
If the chamber temperature set point is 37.0 °C and the high
alarm limit is +1.0 °C, the alarm will trigger at 38.0 °C
(1.0 °C higher than the chamber temperature).
If the chamber temperature set point is changed to
39.0 °C, the high alarm limit will still be +1.0 °C, sothe alarm
will trigger at 40.0 °C (1.0 °C higher than the chamber
temperature).
If an alarm sounds, press
Ö
If an alarm sounds on start up, press to silence it.
to silence it.
To change the high temperature alarm DSection 5.8
To change the low temperature alarm DSection 5.9.
5.8 CHANGING HIGH TEMPERATURE
ALARM
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). +,Wwill show in the left
display.
Press
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
to change the value in the right
display. (Default value is.).
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UPQHZYDOXH
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
5.9 CHANGING LOW TEMPERATURE
ALARM
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.

Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). +,Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Wwill show in the left
display.
Press
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
to change the value in the right
display. (Default value is.).
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UPQHZYDOXH
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
5.10 HIGH AND LOW CO2ALARMS
The incubator has high and low CO2alarm system as
standard. When the chamber CO2level triggers the alarm,
the CO2GLVSOD\ZLOOÀDVKDQGDEX]]HUZLOOVRXQG
The high and low alarm limits are relative to the chamber set
point which means that they do not need adjusting when you
change the chamber temperature. The over and under CO2
alarms will “follow” the changes in your chamber CO2level.
For example:
If the chamber CO2set point is 5.0 % and the high alarm
limit is +1.0 %, the alarm will trigger at 6.0 % (1.0 % higher
than the chamber CO2set point).
If the chamber CO2set point is changed to 10.0 %, the high
alarm limit will still be +1.0 %, so the alarm will trigger at
11.0 % (1.0 % higher than the chamber CO2set point).
If an alarm sounds, press
Ö
If an alarm sounds on start up, press to silence it.
to silence it.
To change the high CO2alarm DSection 5.11
To change the low CO2alarm DSection 5.12.
5.11 CHANGING HIGH CO2ALARM
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). +,Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). +,Fwill show in the left
display.
Press
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
to change the value in the right
display. (Default value is.).
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UPQHZYDOXH
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
5.12 CHANGING LOW CO2ALARM
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). +,Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). +,Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Fwill show in the left
display.
Press
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
to change the value in the right
display. (Default value is.).
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UPQHZYDOXH
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.
Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 9

6 HIGH TEMPERATURE
STERILISATION
Risk of damage to unit.
The CO2Standard D is not intended to be
used as an autoclave and must not be used for
sterilising any other equipment.
Only the shelves, racking and water tray can be
left inside the incubator during decontamination.
Risk of injury. Risk of burning. Internal surfaces
reach 160 °C during the decontamination cycle.
The CO2Standard D incubator is equipped with a
high temperature decontamination function. This raises the
chamber temperature to 160 °C for 4 hours.
Independent evaluation has shown this to be effective
against all spores and bacteria commonly associated with
laboratory incubator contamination.
6.1 ABOUT THE DECONTAMINATION
CYCLE
The decontamination cycle consists of 3 distinct phases:
Phase What happens?
Heat up During the heat up phase, maximum
power is applied to the heating
elements. CO2control is disabled to
protect the sensor.
4 hour dwell The dwell phase starts once the
chamber temperature reaches 160 °C.
Heater power is automatically reduced
to regulate the temperature at 160 °C.
Cool down After 4 hours at 160 °C power is
removed from the heaters and the
cooling phase begins. The chamber
is allowed to cool naturally. When the
chamber temperature reaches 38 °C
the CO2control is re-enabled.
6.2 PREPARATION
Empty and dry the water tray. (The empty water tray
can be left inside the chamber for the duration of the
decontamination incubator if required).
Clear any samples or equipment from the shelves.
The shelves and racking can be left in place during the
decontamination cycle.
Leave the CO2pipe connected.
Instructions for use
10 CO2CELL Standard 190_np_en 1301_mmm_V1.02
5.13 DOOR OPEN ALARM
7KHLQFXEDWRULV¿WWHGZLWKD³GRRURSHQ´DODUPV\VWHPWR
alert you when the outer door has not been closed properly
or when it has been left open for a period of 30 seconds
or more. The 30 second delay timer should be suitable for
most users, but if more time is needed to load and unload
the chamber, the door open timer can be adjusted.
5.14 CHANGING THE DOOR OPEN
ALARM DELAY TIMER
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). +,Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Wwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). +,Fwill show in the left
display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). /2Fwill show in the left
display.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second) GRGwill show in the left
display and a value .in the right display.
Press
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
or
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
to change the value in the right
display. Default value is .which is 30 seconds (ignore
the decimal point ).
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

IRUVHFRQGWRFRQ¿UPQHZYDOXH
Hold
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
Press or 
and
63W
63F
$/
+,W
/2W
+,F
/2F
GRG
or to change the value in the right display.Default value is 
together at the same time to go back
to the main display.

6.3 STARTING THE DECONTAMINATION
CYCLE
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Wwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). 63Fwill show in the left display
and a value .in the right display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). $/will show in the left display.
Press
Ö
If an alarm sounds on start up, press to silence it.
(for 1 second). GH&21will show on the left
displays.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). 5HP2X(WU$\(remove
tray) will show on the displays.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). 6W$UW&\&/H(start
cycle) will show on the displays.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second). $UH<2X6XU("(are
you sure?) will show on the displays.
Press
63W
63F
$/
Press (for 1 second).+,W
/2W
+,F
/2F
GRG

(for 1 second) to start the decontamination
cycle.
The decontamination cycle will start. The cycle timer starts
and counts down from 240 minutes (4 hours).
G(Fwill show on the displays.
The time taken for a full decontamination cycle is
dependent on the cool down phase.
More about decontamination cycle time DSection 6.4.
6.4 CYCLE TIME
As coolingrelieson the natural heat loss through the
chamber walls the time taken is affected by the location of
the incubator and the ambient temperature. So, an incubator
placed under a worktop will take longer to cool than a
freestanding incubator on a worktop.
We recommend that you carry out the decontamination
cycle at the end of a working day. That way, when you return
to work in the morning the incubator will be ready for you to
use.
Thisdecontamination cycle graph showsa typical start-to-
¿QLVKGXUDWLRQRIKRXUV
Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 11
6.5 COOLING PHASE
After the 4 hour dwell is completed the incubator goes into
the cooling phase of the decontamination cycle. All power
to the heaters is removed and the incubator cools naturally.
CO2control is also disabled.
The cooling phase ends once the temperature falls to within
10.0 °C of the working set point (47.0 °C if working set point
is 37.0 °C). At this point normal control of temperature and
CO2resumes.
7 OPTIONS
7.1 VOLT FREE ALARM RELAY (BMS)
The CO26WDQGDUG'LQFXEDWRUVDUH¿WWHGDVVWDQGDUGZLWK
a volt free changeover relay for connection to a building
maintenance system (BMS).
This relay changes state whenever the incubator is in an
alarm condition. The relay changes back to its original state
once the alarm condition clears.
The connections are as shown below:
7.2 MULTI-DOOR (8ID)
7KLVFDQEHUHWUR¿WWHG
The inner glass door can be replaced with a multi-door. This
has 8 smaller glass doors in a stainless steel frame.
By only opening one small door to access the sample you
require, less heat, humidity and CO2are lost.
q q
q

The shelves, racking and water tray can be sterilised in an
autoclave if required.
Clean the glass door and the inner gasket seal.
Clean the inner stainless steel surfaces of the outer door
and the outer door gasket.
9.3 OXIDATION AT HIGH TEMPERATURE
(CO2 STANDARD DONLY)
When operated at 160 °C the action of atmospheric oxygen
may cause discolouration of the stainless steel surfaces.
This natural oxidation is not detrimental to the quality of the
incubator and is completely harmless.
10 SERVICE
10.1 WARRANTY
CO2 Standard D incubators are supplied with a two year
manufacturer’s warranty from the date of installation.
7RUHJLVWHU\RXUZDUUDQW\SOHDVH¿OOLQWKH8VHU*XDUDQWHH
Registration Card and post to MMM or to your local
distributor.
10.2 PREVENTATIVE MAINTENANCE
CO2 Standard D incubators are built to last for many
years, but to ensure that the unit continues to perform
to the highest level we recommend that you purchase a
preventative maintenance contract from MMM.
The incubator should be calibrated annually.
10.3 RETURNING AUNIT
Should your incubator require repair or maintenance then
FRQWDFW\RXUORFDOGLVWULEXWRULQWKH¿UVWLQVWDQFH
Returns to MMM will only be accepted with prior
authorisation from the Service Department.
We recommend that you keep all packaging.
When returning a unit please include the following
information:
xIncubator model and serial number
xDate of purchase
xAuthorisation number
xName of local distributor if not purchased from MMM
xA detailed description of the fault (we can never have too
much information)
xWhere was the incubator located
xContact details of the person who reported the fault
x $FRPSOHWHGGHFRQWDPLQDWLRQFHUWL¿FDWHSOXVDFRS\
faxed through in advance).
MMM cannot accept any returns without a completed
GHFRQWDPLQDWLRQFHUWL¿FDWH
Instructions for use
12 CO2CELL Standard 190_np_en 1301_mmm_V1.02
This improves recovery of the chamber back to ideal
conditions and reduces the amount of CO2used.
8 ACCESSORIES
This is the list of some of the accessories currently
available.
Please contact MMM if the part you require is not listed.
PTSK2 Stacking kit for 2 x Standard 190 / 190D
PTST2 Wheeled platform for Standard 190 / 190D
PRV Single stage CO2pressure reducing valve
PRV2 Two stage CO2pressure reducing valve
RO6 In line CO2reducing valve with gauge
PNEU Automatic change over unit for two cylinders
7<*21 Tygon gas tubing
9 CLEANING
Electrical hazard. Do not spill water or
FOHDQLQJÀXLGVRYHUWKHLQQHURURXWHU
surfaces.
Disconnect before cleaning.
Completely dry the unit before
reconnecting the power.
9.1 OUTER SURFACES
Always disconnect the incubator before cleaning.
It is recommended that the exterior of the incubator regularly
wiped clean. Use a non-abrasive soft cloth dampened with a
warm soapy water solution.
Make sure the exterior is thoroughly dry after cleaning.
9.2 INNER SURFACES
It is advisable to clean the chamber regularly.
Use a 70 % isopropanol 30 % distilled water solution.
Apply using a sterile cloth so as not to introduce any
contamination.
Never use any of the following:
xAny chlorine based detergents or bleaches.
xAny acidic cleaning agents.
xSodium Azide
xIodine
xFerric Chloride
Wipe over the inner surfaces of the stainless chamber, all
the shelves including the underside of the shelves, the shelf
racks and the water tray.

Volume Litres (cu. ft.) 190 (6.7)
Number of shelves 8 shelves Maximum of 8.
SHIPPING DIMENSIONS
Width mm (inches) 925 (36.4)
Height mm (inches) 1080 (42.5)
Depth mm (inches) 850 (33.5)
WEIGHT
Standard 190, Net kg (lbs) 94,5 (208)
Standard 190D, Net kg (lbs) 104 (229)
Standard 190, Shipping kg (lbs) 109 (240)
Standard 190D, Shipping kg (lbs) 117,5 (259)
12.2 DIMENSIONAL DRAWINGS
11 DISPOSAL
The incubator must not be disposed of at
public waste collection points. It must only
be disposed of by licensed waste recycling
companies.
Contact MMM for further information regarding
WEEE regulations.
12 TECHNICAL
INFORMATION
12.1 TECHNICAL DATA
TEMPERATURE MANAGEMENT
Range °C 5 °C above ambient to
60 °C
Control °C 0.1 °C
Stability °C ± 0.1 °C @ 37.0 °C
Uniformity °C ±0.25 °C @ 37.0 °C
Door recovery,
30 s opening
minutes 13
CO2MANAGEMENT
Range % CO20-20 %
Control % CO2± 0.1 %
Stability % CO2± 0.2 %
Uniformity % CO2± 0.2 %
Door recovery,
30 s opening
minutes 6
CO2sensor type NDIR
CO2inlet pressure Bar 0.3 to 0.7
CO2inlet connection type PPSXVK¿WEDUEHGW\SH
HUMIDITY MANAGEMENT
Humidity control type Passive
Range % rH 90 % ± 5 %
Door recovery,
30 s opening
minutes 50
ELECTRICAL DATA
Nominal voltage 50/60 Hz V 230 1N ~PE
Power; Standard 190 W 380
Power; Standard 190D
(decon cycle)
W 1800
Power consumption kWh 0.058
Main fuse F1; Standard
190
5.0 A / 6.3 x 32 mm / 250 V / TT
Main fuse F1; Standard
190D
8.0 A / 6.3 x 32 mm / 250 V / TT
EXTERIOR DIMENSIONS
Width mm (inches) 765 (30.1)
Height mm (inches) 862 (34)
Depth mm (inches) 734 (28.9)
Wall clearance, rear mm (inches) 100 (3.94)
Wall clearance, side mm (inches) 50 (1.97)
INTERIOR DIMENSIONS
Width mm (inches) 632 (24.9)
Height mm (inches) 686 (27)
Depth mm (inches) 440 (17.3)
Instructions for use
CO2CELL Standard 190_np_en 1301_mmm_V1.02 13
DISPOSAL

Instructions for use
14 CO2CELL Standard 190_np_en 1301_mmm_V1.02

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