Motic AE31E Series User manual

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We are constantly endeavouring to improve our instruments and to adapt them to the requirements of
modern research techniques and testing methods. This involves modification to the mechanical
structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction manual, including all specifications are
subject to change without notice.

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Content
1. Nomenclature 4
1.1 Application 4
1.2 Nomenclature 5
1.3 Technical data 7
2. Setting-up the Instrument 8
2.1 Working Environment 8
2.2 Input voltage and power 8
2.3 Illumination 9
2.3.1 Halogen bulb 9
2.3.2 LED module 9
3. Assembling the Microscope 10
3.1 Installing the bulb/ LED module 10
3.1.1 30W Halogen 10
3.1.2 100W Halogen 11
3.1.3 LED module 12
3.2 Filter Holder 14
3.3 Mounting the Condenser 14
3.4 Installing the Objectives 15
3.5 Mechanical Stage 16
3.6 Inserting the Eyepieces 16
4. Microscope Handling 17
4.1 Interpupillary Distance Adjustment 17
4.2 Diopter Adjustment 17
4.3 Coarse and fine focusing 18
4.4 Coarse focus torque adjustment 18
4.5 Brightfield Microscopy 19
4.6 Phase Contrast Microscopy 19
4.7 Filter selection 22
4.8 Brightness control 22
4.9. IR function 23
4.10 Auto light intensity reproduction 23

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5. Photomicrographic Procedure (Only on AE31E TRI) 24
6. Troubleshooting Table 25
6.1 Optical and Operating Problems 25
6.2 Electrical 25
7. Care and Maintenance 26
7.1 Lenses and Filters 26
7.2 Cleaning of Painted or Plastic Components 26
7.3 When Not in Use 26
7.4 Warning Label 27

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1. Nomenclature
1.1 Application
The new Inverted microscope AE31E is representing the ideal instrument for living cells
observation and other microbiological samples. Due to our Colour Corrected Infinity Optics (CCIS)
the AE31E offers superior image quality combined with excellent mechanical performance. Daily
work is facilitated by a number of ergonomic features.

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1.2 Nomenclature
Model AE31E TRI
(Fig.1)

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Model AE31E TRI
(Fig.2)

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1.3 Technical data
Technical data AE31E BIN AE31E TRI
Optical system Color Corrected Infinity Optics (CCIS)
Total Magnification 40X - 400X
Eyepiece tubes
360° Swiveling Siedentopf with upper and lower position: upper
position offers approx. 40 mm extra viewing height
Interpupillary distance: 48 mm to 75 mm
Binocular 45° Inclined Trinocular 45° Inclined
Eyepiece N-WF 10X(Ø22), wide field, high eye point
Nosepiece Quintuple, inclined, sidewards
Stage Fixed, Dimensions (width x depth): 200 x 239mm
Stage height 192mm
Object guide Verniers with numerical and alphabetic scale X direction: numerical scale, readable from right to left
Y direction: alphabetic scale
Objectives
Plan-Achromat 4x, N.A.0.1, W.D.12.6mm
Plan-Achromat 10x, N.A.0.25, W.D.16.8mm
LWD Plan-Achromat 20x, N.A.0.3, W.D.4.7mm
LWD Plan-Achromat 40x, N.A.0.5, W.D.3mm
Plan-Achromat PH 4x Ph0, N.A.0.1, W.D.12.6mm
Plan-Achromat PH 10x Ph1, N.A.0.25, W.D.4.1mm
LWD Plan-Achromat PH 20x Ph1, N.A.0.3, W.D.4.7mm
LWD Plan-Achromat PH 40x Ph1, N.A.0.5, W.D.3mm
LWD Plan-Achromat PH 40x Ph2, N.A.0.5, W.D.3mm
Condenser N.A.0.3, W.D.72mm
N.A.0.5, W.D.28mm
Coarse focus 42mm/rot.
Fine focus 0.2mm/rot.
Transmitted illumination 6V/30W Halogen 12V/100W Halogen
3W LED module
Dimension: W x D x H 217.5 x 556 x 497mm

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v2. Setting-up the Instrument
2.1 Working Environment
The location should be free from dust, moisture, chemical vapours and free of mechanical
vibrations.
Don't locate the instrument in bright or direct ambient light, in front of a lamp, or a bright light wall.
Best imagequality will be achieved in a surrounding surrounding with low ambient light.
Environmental specification:
Indoor use
Altitude: Max 2000m
Ambient temperature: 15°C~ 40°C;
Maximum relative humidity: 75% for temperature up to 31°C decreasing linearly to 50% relative
humidity at 40°C
Supply voltage fluctuations: Not to exceed ±10% of the normal voltage
Pollution degree: 2 ( in according with IEC60664)
Installation/Overvoltage category: 2 (in according with IEC60664)
Air Pressure of 75kPa to 106 kPa
2.2 Input voltage and power
Automatic voltage selection works with electrical outlets worldwide. It is advised to always use a
power cord that is rated for the voltage used in your area and that has been approved to meet
local safety standards. Using the wrong power cord could cause fire or equipment damage.
In order to prevent electric fluctuation to the instrument electrics, always turn the power switch on the
instrument off before connecting the power cord.
Electrical Specifications:
30W Halogen bulb
Input: 100-240V~, 45W, 50-60Hz
Lamp: DC6V 30W Halogen
Fuse: 250V T2.5A
(If the original fuse is damaged, please replace a new one with same specification)

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100W Halogen bulb
Input: 100-240V~, 120W, 50-60Hz
Bulb: 12VDC 100W Halogen
Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)
LED module
Input: 100-240V~, 80VA, 50-60Hz
LED: 3.4V 3W
Fuse: 250V T2.5A (If the original fuse is blown, please replace with specified fuse)
LED module high color temperature: 6000 ± 300k
LED module low color temperature: 4500 ± 300k
Attention: The plug of the power adaptor is the "disconnect device" for whole unit. To save energy we
recommend to unplug the instrument when not in use.
This device complies with Part 15 of the FCC Rules.
Operation is subject to the following two conditions:
(1) This device may not cause harmful interference, and (2) this device must accept any interference
received, including interference that may cause undesired operation.
2.3 Illumination
2.3.1 Halogen bulb
The quartz halogen bulb, used as a light source, has higher luminance and colour temperature
than conventional tungsten lamps. The luminance of halogen bulb is approximately four times
brighter than the conventional tungsten lamps.
As long as the lamp voltage is kept constant, the halogen lamp maintains the same level of
brightness and colour temperature regardless of whether it is new or nearing the end of its
life span.
2.3.2 LED module
The LED module is specially designed to be inserted into halogen bulb socket directly converting
halogen illumination to LED illumination. LED is more economical and environmental friendly and
combines the advantages of low heat and long life span.