Motic BA80 Series User manual

1
We are constantly endeavouring to improve our instruments and
to adapt them to the requirements of modern research techniques
and testing methods. This involves modification
to the mechanical structure and optical design of our instruments.
Therefore, all descriptions and illustrations in this instruction
manual, including all specifications are subject to change
without notice.

2
Table of Contents
Name of Components 3
Introduction 4
Unpacking 5
Description of Components 6
Setting up the instrument 7
Assembly 8
Operation 9
Maintenance 14
Troubleshooting 18
Moving the microscope 20
Repair 20
Warning Label 21
Warrantee 21

3
Name of Components
BA80 (Monocular)
1. Eyepiece 9. Base
2. Revolving nosepiece 10. Eyepiece tube
3. Objectives 11. Head
4. Fixed stage clip 12. Arm
5. Diaphragm 13. Knobs controlling movement
6. Stage 14. Fine focus knob
7. Condenser 15. Light intensity control
8. Illuminator

4
Introduction
Thank you for your purchase of a Motic microscope. Motic microscopes are
precision instruments, subjected to stringent quality control in order to reach
you in perfect condition. Their design combines easy management and
optimum functioning with minimum maintenance.
The information contained in this manual is likely to go beyond what the
average user needs to know to use the microscope, however, it is provided to
answer any queries that may arise.
Your new microscope combines high performance features, with an excellent
degree of optical resolution and clarity of image. Some models incorporate a
mechanical stage which provides a travel range of 75mm x 35mm in X and Y
directions with a graduation of up to 0.1 mm, thus permitting the perfect
positioning of the specimen. Also included are objectives located in a
nosepiece allowing movement in both directions; a precision coarse and fine
focusing system; accurate condensers with a numerical aperture of 0.65 N.A.
or 1.25 N.A. and LED illumination with variable intensity control.
These instructions should be read carefully before operating the microscope.
They will permit you to use your new microscope to its fullest capabilities.
Terminology used to describe components and controls can be found in the
diagram on page 2.
These instructions are based on the assembly and use of the BA80 model
with additional notes applying specifically to other models in the series.

5
Unpacking
All components of the microscope have been carefully packed to ensure they
reach you in perfect condition. We recommend that you do not discard any
packing containers in case you need to return the microscope, store it for long
periods of time; or should it become necessary to transport it to a technical
service for any repair, or maintenance procedure.
The box should contain the following components, depending on the model:
A microscope assembled with a monocular head, an eyepiece, a fixed
stage, 0.65 N.A. condenser or 1.25 N.A. Abbe condenser and three
objectives or four objectives.
Additional components are: a blue filter, a dust cover, and two hexagonal
keys measuring 2mm and 0.85mm.
Remove, and handle the microscope and all its components with
extreme care.
Avoid touching the lenses of the optical elements and keep clear of
contact with dust, water or other contaminating agents, as they could
stain, or damage the lens surface and affect the quality of the image.
A. Place the microscope in an upright position on a flat, stable and
clean surface.
B. Remove the rest of the components from the box.

6
Description of Components
1. Head — Monocular head rotating 360º to avoid the necessity of moving the
microscope, should another user wish to use it.
2. Eyepiece — The group of lenses closest to the eye, magnifying the image
formed by the objectives. In the monocular models, the eyepiece contains a
pointer to single out any particular element of the sample to another user.
3. Revolving nosepiece — The revolving action permits the user to change
the degree of magnification, the correct positioning of the objectives is
marked by a “click” in the optical path.
4. Objectives — The group of lenses closest to the sample, or microscopic
specimen forming the primary magnified image.
5. Stage — Platform of the microscope where the specimen is placed. Models
with plain stage where specimen slide is held in place by specimen holder
clips. In other models, a mechanical stage replaces the clips, and permits
precise, mechanical manipulation of the specimen slide.
6. Condenser — Optimises illumination for enhanced resolution and
image contrast.
7. Focusing Knobs — Situated on both sides of the arm of the microscope,
the larger, or coarse focusing knob initially brings the specimen into focus,
and the smaller, fine focusing knob permits a precise adjustment of
the image.
8. Illumination — LED illumination with intensity control knob.

7
Setting up the instrument
Avoid placing the instrument in locations exposed to direct sunlight,
dust, vibration, high temperature and high humidity
1 Operating Enviroment
 Indoor use.
 Altitude : Max 2000 meters
 Ambient temperature : 15°C to 35°C
Maximum relative humidity : 75% for temperature up to 31ºC decreasing
linearly to 50% relative humidity at 40ºC
 Supply voltage fluctuations : Not to exceed ±10% of the normal voltage
 Pollution degree : 2 (in according with IEC60664)
 Installation / Overvoltage category : 2 (in according with IEC60664)
 Air Pressure of 75kPa to 106kPa
 No hoar frost, dew, percolating water, rain
2 Verifying Input voltage
 The automatic voltage selection works with a broad range of settings.
However, always use a power cord that is rated for the voltage used in your
area and that has been approved to meet local safety standards.
Using the wrong power cord could cause fire or equipment damage.
In case of using the extension cord, use only a power supply cord with a
protective earth (PE) wire.
In order to prevent electric shock, always turn the power switch on the
power supply off before connecting the power cord.
Input : 110V~ 60Hz
Lamp: 3.1V 60mW LED
Fuse : 125V T0.5A
OR
Input : 220V~ 50Hz
Lamp: 3.1V 60mW LED
Fuse : 250V T0.25A

8
Warning: Before connecting the microscope to an electrical source, always
check that the voltage coincides with that of the microscope.
Assembly
All the steps described for the assembly of the microscope must be
undertaken with extreme care, and without forcing the placement of the
distinct parts and elements of the microscope.
A. Specimen holder mechanism for models with built in mechanical stage:
Rotate the coarse focusing knob to move the stage to its lowest position.
Remove the two knurled screws of the specimen holder mechanism. Place
the mechanism on the stage with the moveable clip lever facing outwards,
and making sure that the holes of the mechanism, and the knurled locking
screws coincide, screw down firmly.

9
Operation
A. Starting Up
1. Before using the microscope, adjust the light intensity control to minimum
position. This should be repeated every time the microscope is switched
on or off to prolong the use of the bulb.
2. Press switch to position ON. (Fig. 1)
Fig. 1
3. Rotate light intensity dial until the image is illuminated.
4. Light intensity should be adjusted in accordance with the objective
used, or the type of specimen examined.
* Using LED illuminating microscope (Rechargeable batteries)
Fig. 2

10
1. Charge the rechargeable batteries before using. Even if the rechargeable
batteries are not charged, you can use the microscopes as long as the
charger is connected and turned on.
2. To maintain a longer life span of the rechargeable batteries and to save
energy, switch off the power supply if not in use.
* For optional accessories only.
B. Focusing the microscope
1. Revolve the nosepiece to place the objective 4X in the optical path,
checking that it is clicked into place.
2. Turn the coarse focusing knob until the stage is set to its lowest position.
3. Place the microscopic specimen slide on the stage, making sure that the
cover slip is raised.
 Swing out the moveable stage clip on the mechanical specimen holder,
support the specimen slide against the fixed stage clip and gently
release the moveable clip until the preparation is well fixed into place.
 For models with plain stage: Raise the specimen holder clips by
putting downward pressure on the back part, slide the specimen slide
under the clips, and release pressure so that the slide is well fixed
into place.
4. Insure that the specimen to be examined is in the optical path. To do
so, adjust the knobs controlling the X/Y movement of the stage or
manually for models with plain stage.
5. Looking through the eyepiece, adjust the coarse focus knob until the
specimen appears in focus.
6. Readjust the focus with the fine focus knob until the image appears in
sharp focus.

11
C. Adjusting the aperture of the diaphragm
The diaphragm should not be used to regulate the light intensity. Its function
is to obtain a high resolution of the specimen and to provide contrast in the
image. Smaller apertures will deliver higher contrast to image, although
closing the aperture too much will reduce resolution. The best method to
obtain the correct aperture of the diaphragm is to experiment. Suggested
apertures for each objective are as follows:
OBJECTIVE APERTURE OF IRIS
4X From fully closed to 1/8 open.
10X From 1/8 to 1/4
40X From 1/4 to 1/2
100X (optional) From 1/2 to 3/4
D. Changing magnification
1. Position the objective 10X in the optical path.
2. This microscope has already been parfocalised, although it is possible
that small differences exist between the objectives. It may then be
necessary to adjust the focus slightly with the fine focus knob.
3. When changing to the 40X and 100X objectives (optional), it must be
done with great care, making sure that the objectives do not scratch with
the specimen slide causing damage to the front lens.
4. In order to obtain maximum resolution of the 100X (optional), it is
necessary to apply immersion oil between the cover slip of the slide and
the front lens of the objective.
a. Only a very small amount of immersion oil is needed, a drop should
be enough.
b. If air bubbles appear they can be removed by gently rotating the
nosepiece back and forth.

12
c. When viewing is complete, all parts that have come into contact with
the oil must be cleaned using a soft cotton cloth, lightly dampened
with Xilene. If the 100X objective is not cleaned, the oil will dry, and it
will not be possible to see through it; permanent damage could
also occur.
NB. Immersion oil must ONLY be used with the 100X objective, as it is the
only one specially prepared for it. If any other objective comes into
contact with the oil, it must be cleaned immediately.
E. Critical illumination for models with focusable 1.25 N.A.
Abbe condenser
The ideal level of illumination is when all illumination elements are brought
into proportion, basically, by the condenser. To achieve critical illumination
an object over the illuminator must be in focus.
1. Focus on slide with the 10X objective.
2. Place a flat object on the illuminator. The object must be one that lets
light through, a slide for example.
3. Without letting go of slide, focus, using the other hand to turn the collar to
move the condenser.
4. When critical illumination is achieved, the slide can be removed. If any
irregularity appears in the field of view, i.e. an optical element from the
focussed illuminator, move the condenser just enough to take it out of
focus, thus obtaining the best level of illumination possible, and
closest to critical illumination.

13
WARNING: FOR YOUR OWN SAFETY, SWITCH OFF AND
DISCONNECT THE MICROSCOPE FROM ANY ELECTRICAL SOURCE
BEFORE ATTEMPTING ANY MAINTENANCE PROCEDURE TO AVOID
THE RISK OF ELECTROCUTION.
IF THE MICROSCOPE REQUIRES ANY MAINTENANCE OR REPAIR
NOT APPEARING IN THIS MANUAL, CONSULT YOUR DISTRIBUTOR.
Maintenance
A. Optical maintenance.
Do not attempt to disassemble any optical component.
Prior to cleaning any of the lens surfaces, remove dust particles using a fine
brush, specifically for cleaning lenses. Alternatively, use low pressure
compressed air, available in shops selling photography equipment.
1. Cleaning the eyepiece.
a. Do not remove eyepiece from eyepiece tube.
b. Clean only the outer surface, misting the lens with breath.
c. Dry by wiping with lens paper in circular movements, from centre,
outwards. Do not wipe lenses when dry, as they are easy to scratch.
2. Cleaning the objectives.
a. Do not remove the objectives from the microscope.
b. Only clean the outer surface, dampening a soft cotton cloth slightly
with Xylene then drying the lens with the same cloth.
3. Cleaning the condenser.
a. Clean only the top lens surface using either of the above methods, as
for the eyepiece, or the objectives.
4. Cleaning the illuminator lens.
a. Use any of the above methods, as for eyepiece, or the objectives.

14
B. Illumination maintenance
1. Replacing the LED
a. To open the LED lamp housing, please use the Allen key provided as
shown in Fig.2 to loosen the screw on the side of the lamp housing
and pull the housing up Fig.3.
Fig.3
b. The LED lamp comes with its holder, as shown in Fig.4, which should
be plugged onto the electrical connecter, as shown in Fig.5
Fig.4 Fig.5
c. After opening the lamp housing, pull out the LED lamp with its socket,
replace with a new one, as shown in Fig.6

15
Fig.6
d. Put back the lamp housing on its holder and secure it with the original
Allen screw and Allen key provided.
C. Mechanical maintenance
1. Adjusting the tension of the coarse focus knob.
The collar to adjust coarse focus tension (Fig.7) is situated between the
coarse focus knob and the arm. Coarse focus tension is adjusted by the
factory. The optimum point of tension is that which permits the lightest
movement of the coarse focusing knob possible, without the stage
sinking from its own weight.
a. To adjust tension, first loosen the slotted set screw in the collar hole
with a 2mm hexagonal key.
b. To tighten the tension of the coarse focus knob, turn the collar anti-
clockwise; or to loosen it, turn clockwise.
c. Tighten the hexagonal screw.

16
Fig.7
2. Adjusting the rack stop.
40X and 100X(optional) objectives use a retractable security system to
avoid damage to the specimen slide or to the front of the lens, should the
two come into contact. Additionally, as a security measure, the
microscope includes a rack stop screw that regulates the upward
movement of the stage. The rack stop screw comes pre-adjusted by the
factory for standard slides with a 0.17mm thick cover slip. However, for
observing other types of slides or for using polarising equipment
(optional), adjustment may be necessary.
a. Loosen the rack stop screw (Fig. 3) with the 2mm key.
b. With the fine focus adjustment at mid-range, focus on specimen slide,
using only the coarse focus knob, firstly with the 4X objective, then
with the 10X.
c. Rotate the rack stop screw until tight enough to prevent the stage
rising further.

17
Troubleshooting
ELECTRICAL PROBLEMS
Problem Cause Solution
The bulb does not work Outlet inoperative. Repair through a
qualified technician.
Cable not connected. Connect.
Bulb burned out. Replace bulb.
The bulb burns out in a
short time Very high voltage. Reduce light to minimum before turning
microscope on or off.
The bulb burns out
immediately Wrong bulb. Replace with the correct bulb.
The bulb flickers The bulb is not inserted
correctly in the socket. Insert it correctly.
The bulb is at the point
of burning out. Replace bulb.
The fuse cap is not
closed properly. Close correctly.
Bad outlet connection. Repair through a
qualified technician.

18
IMAGE QUALITY
Problem Cause Solution
No image. Nosepiece badly
positioned. Turn, until it clicks into position.
Image too bright. Reduce light intensity.
Poor resolution. Objective lens dirty. Clean objective.
Eyepiece lens dirty. Clean eyepiece.
Specimen slide up
side down. Place slide with slip facing up.
Cover slip on specimen
slides wrong thickness. Use 0.17mm thick cover slip.
Light too bright. Reduce light intensity or
adjust diaphragm
aperture.
Condenser dirty. Clean condenser.
Spots in field
of view Dirty eyepiece. Clean eyepiece.
Dirty slide. Clean slide.
Dirty condenser. Clean condenser.
Uneven illumination
of field Nosepiece not properly positioned. Turn until clicks into position.
Diaphragm not properly
positioned. Adjust accordingly.

19
MECHANICAL PROBLEMS
Problem Cause Solution
Does not stay in focus. The stage drops down. Adjust coarse focus
tension.
Does not stay in focus. The rack stop of the
ascending movement of
the stage is badly
adjusted.
Readjust rack stop
screw.
Moving the microscope
 If possible, avoid moving the microscope.
 Carry the microscope in both hands. One hand should hold the microscope
arm, and the other should support it under the base.
 Maintain the microscope in a vertical position.
Repair
If the microscope needs repairing, or revision by authorised personnel, we
would recommend that it be stored in its polystyrene box and returned to the
distributor. Attach a note with a description of the problem, or details of the
required revision.
Note:
If equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.