MP Biomedicals FastPrep-24 User manual

USER MANUAL For Research Use Only
FastPrep-24™ Classic
www.mpbio.com
Cat. No. 116004500
Revision Date: #116004500-042523
The FastPrep-24™ Classic Instrument is a high-speed benchtop homogenizer oering
the ulmate in speed and performance for the lysis of biological samples.
Simultaneous homogenizaon of up to 24 samples takes place within 40 seconds.
Circle frame for
applicaon images
(oponal)

USER MANUAL: FastPrep-24™ Classic
2
TABLE OF CONTENTS
1. Secon Title Here add tabs before and aer secon tles............#
1.1 Subsecon Title Here. Add forced line break + tab
before text in second line for cleaner look .....................#
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 beforetextinsecondlineforcleanerlook ..................#
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2.1.1 Sub-SubseconTitleHere. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . #
2.1.2 Sub-SubseconTitleHere. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . #
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The FastPrep-24TM is CE in compliance with the
European Low Voltage and EMC Direcves

3
1. PRECAUTIONARY INSTRUCTIONS
The precauonary instrucons found in this secon and throughout the
manual are indicated by specic symbols. Understand these symbols and
their denions before operang this equipment. The denion of these
symbols are as follows:
Text with a “CAUTION” indicator will explain
possible Safety infracons that could have the
potenal to cause minor to moderate injury or
damage to equipment.
Text with a “WARNING” indicator will explain
possible Safety infracons that will potenally
cause serious injury and equipment damage.
NOTE: Throughout this manual “NOTE” may be found. These notes
are helpful informaon to aid in the parcular area of funcon being
described.
= WARNING
= CAUTION
= CAUTION
Read, understand and pracce the precauonary and operang instrucons.
Know the limitaons and hazards associated with using this unit. Observe the
precauonary and operaonal decals placed on the unit.
DO NOT operate this unit in an environment where other devices are being used that
intenonally radiate electromagnec energy in an unshielded manner.
DO NOT use sharp objects such as a pencil point or ballpoint pen to operate the buons
on the control panel as damage may result.
This unit should be operated in temperatures between 15°C to 35°C, transported
and stored in temperatures between 5°C to 43°C, with relave humidity ranging
from 30%–60%.
DO NOT disassemble, modify or remodel the unit or accessories.
= WARNING
This device should be kept out of the reach of children.

USER MANUAL: FastPrep-24™ Classic
4
2. INTRODUCTION
The FastPrep-24TM Classic instrument is a high-speed benchtop
homogenizer oering the ulmate in speed and performance for the lysis
of biological samples. Simultaneous homogenizaon of 24 samples in 2
mL tubes, 12 samples in 15 mL tubes, 48 samples in 2 mL tubes or 2
samples in 50 mL tubes takes place within 40 seconds.
The FastPrep-24 Classic instrument uses a unique, opmized
moon to disrupt cells through the muldireconal,
simultaneous beang of specialized Lysing Matrix beads
on the sample material.
Developed for dicult and resistant samples, the FastPrep-24 Classic
instrument thoroughly and quickly lyses any ssues and cells. It allows
easy and reproducible isolaon of stable RNA, acve proteins and full-
length genomic DNA. Samples and buers are added to a Lysing Matrix
tube containing specialized Lysing Matrix beads. The ergonomic design
of the instrument ensures easy loading of the sample tubes and secure
sealing during processing. The homogenizaon speed and duraon mes
are digitally controlled. Aer seng your speed and me with the touch
of a buon, just push “run”, and in less than a minute, your samples are
completely lysed.
Fig. 1 (front view)
Dome
Sample Tubes Holder
Bowl
Securing Knob (ratchet)
Spoke Plate
Micro Switch
(under Dome)
Dome Clip
(Dome Fastener)
Enclosure
Front Panel Emergency Switch

5
3. INSTALLATION
3.1 Unpacking
Carefully remove the FastPrep-24 instrument and accessories from the
shipping carton. In addion to the instrument, the following accessories are
included.
Item Quanty
AC Power Cord US / EU 2 (1 US, 1 EU)
Dome Clip 2
Fuse 10 Amp 2
Instrucon Manual 1
Securing Knob (ratchet) 1 pack
Compare the packing list to the box contents. If there is a discrepancy, please
nofy MP Biomedicals.
CAUTION: DO NOT LIFT DEVICE BY COVER
3.2 Inspecon
Inspect the unit for any damage that may have occurred during shipment.
Should there be any damage, report it to the carrier and contact MP
Biomedicals immediately. Save the packaging material in the event a return is
necessary.
3.3 Set-up
To assure safe operaon and best results, read this manual before operang
the FastPrep-24 instrument. The FastPrep-24 instrument comes fully
assembled, requiring very lile set-up. Install the system on a clean, dry,
stable surface within 4 feet (1.2 m) of a compable electrical outlet.

USER MANUAL: FastPrep-24™ Classic
6
3.4 Venlaon
Allow 1-2 inches (3-5 cm) of space around the FastPrep-24 instrument for
proper venlaon. This unit is FOR INDOOR USE ONLY. Avoid operang in
areas of excessive humidity or temperature extremes.
3.5 Connecng the Power
The FastPrep-24 can work on 110 VAC/60 Hz or 220 VAC/50 Hz. Make sure
the rocker switch located on the rear panel is OFF when connecng the
power. Connect the power cord to the instrument (power xture is at the
back side) and plug it into a compable outlet.
This symbolizes Alternang Current
4. OPERATION
4.1 Controls and Funcons
Funcons of control panel keys are provided below (Ref. Fig. 2)
1. SET Key: Press this key to select Speed &
Time as displayed on LCD and to choose
program (model 6004)
Speed: Selectable from 4.0 m/s to 6.5 m/s.
In steps of 0.5 m/s (Default 4.0 m/s).
Tube holder: Selectable list of tube holders
Time: Selectable from 1 sec to 60 sec in
increments of 1 sec. (Default 20 sec)
2. RUN Key: To start or stop the instrument.
3. Key: To increase the selected value of
speed, tube holder and me.
4. Key: To decrease the selected value of
speed, tube holder and me.

7
4.2 Loading and Securing the Samples
1. Loosen the Securing Knob (Ratchet Nut) by rotang counter-
clockwise unl it comes out (Ref. Fig. 3).
2. Remove the Securing Knob (Ratchet Nut).
3. Take out the assembly of Spoke Plate and Tube Holder (Ref. Fig. 6)
Fig. 3
Fig. 4
Three Step
Tube Holder
Securing Knob
(Ratchet Nut)
Spoke Plate
Spoke Plate

USER MANUAL: FastPrep-24™ Classic
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4. Slightly li the Spoke Plate and rotate clockwise so that the retenon
spokes move away from holes to leave them open for loading (Ref. Fig. 5).
Fig. 5
Fig. 6
5. Load sample tubes into the cavies of the Sample Holder so that they t
snugly in the holes. Posion the tubes symmetrically.
6. When all the sample tubes are loaded, place the assembly into the
FastPrep-24™ instrument. Align the Locking Pin of the Aluminium Three
Step with the holes under the Tube Holder, ensuring its proper placement
(Ref. Fig. 6).
Sample Hole
Aluminium
Three Step
Sample Holder
Spoke Plate
Hole under
the tube (for
matching)
Locking Pin

9
7. Upli and rotate the Spoke Plate counter-clockwise so that the retenon
spokes take the posion above each sample tube cap. Place the Securing
Knob (Ratchet Nut) on the assembly to ghten it completely (Ref. Fig. 7).
The Securing Knob should be ratcheted several mes (not just once). It is
good pracce to push down on the nut while twisng to secure the part,
make sure you hear several clicking sounds.
Fig. 7
8. Close the Dome properly and secure the Dome Clip.
WARNING: Sample tubes must be secured properly before
running the FastPrep-24 instrument
Spoke Plate
Sample Tube

USER MANUAL: FastPrep-24™ Classic
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4.3 Preparing the FastPrep-24 for Operaon
1. FastPrep is delivered with an Emergency Switch. Remove the Emergency
Switch by pulling and then turn the main Rocker Switch ON (backside of the
machine). It is not necessary to pull out the Emergency Switch each me the
instrument is used - simply use the Rocker Switch.
2. Factory-set default values automacally program FastPrep-24 to operate
at Speed: 4.0 m/sec, Tube Holder: MP:24x2 and Run Time: 20 seconds (to
override these values, see programming).
4.4 Programming the FastPrep-24
1. Switch ON Power Supply using Rocker Switch provided at the back.
Ensure Emergency Switch is pulled out.
2. On Power ON, the LCD will show WELCOME followed by MP Bio
3. User can accept the default seng or program their own. Five dierent
combinaons of sengs can be saved as described in the next secon.
4. Press SET key to start programming.
5. Once SET key is pressed, LCD will show the default seng speed of 4.0 m/s.
If Opon 1 is needed, then press SET key again. The 4.0 m/s message on
LCD will start ashing to indicate that it can be changed at this moment.
Press or key to change the default Speed to User Selectable Speed. Once
speed is selected, press SET again. Flashing of 4.0 m/s will stop to indicate
that speed has been selected.
This symbolizes ON Supply
This symbolizes OFF Supply

11
1. To change the default Tube Holder seng, press SET key. LCD will show
4.0 m/s Use key unl MP:24x2 is displayed, then press SET key. LCD will
start ashing to indicate the sample tube holder can be changed using or
key. Once sample tube holder is selected, press SET key again. Flashing of
LCD will stop to indicate that Tube Holder has been selected.
NOTE: Duringtubeholderselecon,LCDwillbedisplayedas:
MP:24x2 QuickPrep
TM Adapter (supplied with instrument)
BG:2x50 BigPrep
TM Adapter (2 x 50 mL tubes)
TN:12x15 TeenPrep
TM Adapter (12 x 15 mL tubes)
CY:24x2 CryoPrep
TM Adapter (24 x 2 mL tubes)
HG:48x2 HighPrep
TM Adapter (48 x 2 mL tubes)
CUSTOM Custom holder (all other adapters)
2. To change the default Time seng, press SET key. LCD will show 4.0 m/s.
Use key unl TIME 20 is displayed, then press SET key. LCD will start
ashing to indicate the Run Time can be changed using or key. Once Run
Time is selected, press SET key again. Flashing of LCD will stop to indicate
that Run Time has been selected.
3. Once Speed, Tube Holder and Run Time have been selected or saved, press
RUN key. LCD will show SURE? . Press RUN key to start the session.
4. If you are ready to start, press the RUN key again. The motor will start and
display will show count down run me.
5. When the me is over, LCD will display REST 5 mn indicang that the
motor is at rest for 5 minutes. Display will count down each minute unl the
LCD displays READY . The program will go back to main menu with last
selecon.

USER MANUAL: FastPrep-24™ Classic
12
NOTE: When Speed, Tube Holder or Run Time is ashing (in selectable mode),
the user can sll start the unit directly by pressing RUN key twice. These values
will be temporary and they will not be saved unless SET key is pressed and LCD
stops ashing.
1. Press RUN key to stop the instrument at any me during a run. LCD will
show Stop . The program will go back to main menu with last selecon.
2. Turn OFF the instrument by pressing Rocker Switch.
4.5 Save Programmed Combinaons
During Program 1 to 5, Tube Holder selecon will be displayed as:
PX TH MP QuickPrep
TM Adapter (supplied with instrument)
PX TH BG BigPrep
TM Adapter (2 x 50 mL tubes)
PX TH TN TeenPrep
TM Adapter (12 x 15 mL tubes)
PX TH CY CryoPrep
TM Adapter (24 x 2 mL tubes)
PX TH HG HighPrep
TM Adapter (48 x 2 mL tubes)
PX TH CS Custom holder (all other adapters)
MP:24x2 QuickPrep
TM Adapter (supplied with instrument)
BG:2x50 BigPrep
TM Adapter (2 x 50 mL tubes)
TN:12x15 TeenPrep
TM Adapter (12 x 15 mL tubes)
CY:24x2 CryoPrep
TM Adapter (24 x 2 mL tubes)
HG:48x2 HighPrep
TM Adapter (48 x 2 mL tubes)
CUSTOM Custom holder (all other adapters)
PX = Program 1 to 5
1. If Save Mode 1 to 5 is required, press SET key. LCD will show 4.0 m/s .
Use key unl Program 1 is displayed, then press SET key. Display will
show P1 S 4.0 and the value “4.0” will be ashing, indicang the speed set
in Program 1 is 4.0 m/s. Use or key to change Speed value.

13
CAUTION
Improper closing of Dome will be indicated by display Cancel
Please do not run the instrument without the Tube Holder, as this may cause damage
to the motor sha
To prevent sample overheang, instrument must rest for 5-10 minutes between runs.
The instrument should be properly grounded for safe use.
Temperature Indicaon:
If LCD displays SYS WARM it is an indicaon that crical parts of the system are
becoming hot. User should provide a pause before next run.
If LCD displays SYS HOT! it is an indicaon that temperature of crical parts is high.
Unit will stop automacally and user will not be able to make any run unl the
temperature decreases to a safer value.
Overload Indicaon:
If there is a wrong selecon of Tube Holder or an overload condion, LCD will display
OverLoad . This is an indicaon that user must make proper selecon of Tube Holder
or reduce the number of tubes in Tube Holder.
2. Once Speed has been set, press SET key to save in program 1. Now the LCD
will show P1 TH MP . Leer “MP” will be ashing. Use or key to set the
desired Tube Holder.
3. Once Tube Holder has been set, press SET key to save in Program 1. Now
the LCD will show P1 T 20 . Value “20” will be ashing. Use or key to
set the desired Run Time. Press SET key to save the Run Time in Program 1.
LCD will display Program1 . Follow same instrucons to save in Program 2
to 5.
EMERGENCY NOTE: In the event of an emergency, push the Emergency Switch
to stop the instrument at any me (on the front panel of the instrument). The
instrument will be completely isolated from AC mains. Pull the Emergency Switch
again to make the instrument ready to restart.
Earth Terminal

USER MANUAL: FastPrep-24™ Classic
14
5. SPECIFICATIONS
Controls Programmable run me, tube holder and speed;
LCD readout
Time Range: 1-60 sec
Speed Range: 4.0-6.5 m/s
Acceleraon <2 seconds to maximum speed
Deceleraon < 2 seconds to stop
Weight 17.9 kg
Power Requirement 110 VAC/60 Hz or 220 VAC/50 Hz, 500 W
Operang Temperature 5°C to 35°C
Storage Temperature 5°C to 43°C
Dimensions (L x W x H) 33.2 cm x 43.7 cm x 46.5 cm

15
6. WARRANTY & LIABILITY
WARRANTY
The product warranty extends to the original consumer/purchaser of the
product.
WARRANTY DURATION
This product is warranted to the original consumer for a period of one (1) year
from the original purchase date. Extended warranty duraon is available upon
request.
WARRANTY COVERAGE
This product is warranted against defecve materials. The warranty ceases if
the product has been damaged by accident, in shipment, unreasonable use,
misuse, neglect, improper service, commerical use, repair by unauthorized
personnel or cause not arising out of defect in materials or workmanship. This
warranty does not extend to any units which are used in violaon furnished
by manufacturer, or to units which have been altered or modied, or machine
installed in a contaminated area with no removing possibility, or to damage to
products or parts there of which have had the serial number removed, altered or
defaced or rendered illegible. The warranty doesn’t cover normal wear and tear
or replacement of electrical cord, springs, rubber cups, dome, tube holders, tube-
covers, motor and its aachments and other accessories.
WARRANTY DISCLAIMERS
The warranty is in lieu of all warranes expressed or implied and no
representave or person is authorized to assume for manufacturer/any other
liable in connecon with the sale of our products. There shall be no claims for
defects or failure of performance or product failure/any theory of tort, contract
or commerical law including, but not limited in negligence, gross negligence,
strict liability, breach of warranty and breach of contract. Some states do not
allow the exclusion or limitaon of implied warranes or consequenal damages
arising out of or in connecon with the use performance of the product or other
damage with respect to loss of property or loss of revenues or prot.

USER MANUAL: FastPrep-24™ Classic
16
LEGAL REMEDIES
This warranty gives you specic legal rights, and you may also have other rights
that vary from state to state.
WARRANTY PERFORMANCE
During the above one-year warranty period, a product with a defect will be
repaired or replaced with a recondioned comparable unit at MP Biomedicals
opon when the product is returned to MP Biomedicals nearest facility or
distributor. The repaired or replacement product will be in warranty for the
balance of the one-year warranty period and an addional one-month period. No
charge will be made for such repair or replacement.
WARRANTY SERVICE
For in-warranty service for a product covered under the warranty period, no
charge is made for service. Please return the product insured, packed with
sucient protecon in original box, postage insurance, prepaid to the address.
No instrument returns can be accepted without a return authorizaon (RGA) Any
duty/brokerage fee, if any, must be paid by the costomer.
OUT OF WARRANTY SERVICE
There will be charges rendered for repairs made to the product aer the
expiraon of the aforesaid one (1) year warranty period, aer purchaser is
advised appropriately. The distributor cannot assume responsibility for loss
or damage during shipment. For your protecon, carefully pack the product
for shipment and insure it with the carrier. Ensure that you return the unit
and accessories related to your problem and also that you indicate full return
address. C.O.D. shipments cannot be accepted.

17
7. APPENDICES
7.1 APPENDIX 1: Maintenance & Cleaning
Maintenance: The FastPrep-24 instrument requires no scheduled maintenance.
Clean surfaces immediately aer contact with sample soluons or reagents.
Fig. 8 (Spring Replacement)
Spring Replacement Instrucons:
For the long running life of the instrument, 8 extra springs are provided. If a
spring breaks, follow the steps below (Ref. Fig. 8):
1. Open the Dome and Securing Knob (Ratchet Nut) from the sha.
2. Remove the sample holder and bowl (all plasc pieces).
3. Put the screw driver through the hole provided on the face of aluminium
collared bush holding and unscrew the boom spring holder and top end of
spring’s screw.
4. Remove damaged spring and replace it with a new spring. It is recommended
that both springs be replaced at the same me.
5. Now reassemble the instrument in reverse order.

USER MANUAL: FastPrep-24™ Classic
18
Fuse Replacement:
Fig. 9 (Fuse Replacement)
Rocker Switch
Fuse Receptacle
AC Receptacle
CAUTION: Disconnect input power before replacing fuse. For connued
re protecon, replace only with specied type and rated fuse. Fuse rang is
10 AMP. 2 extra fuses are provided with accessories. If a fuse blows, follow
the steps below for replacement (Ref. Fig. 9)
1. Ensure that input power is disconnected during replacement of fuse.
2. Take out fuse receptacle from the AC receptacle provided at the back side
of the instrument. A spare fuse is provided in fuse holder. Remove the faulty
fuse and replace with new one.
3. Insert the fuse receptacle into the AC receptacle carefully.
4. Connect the power cord to the instrument and plug it into a compable
outlet and turn ON the rocker switch.

Fig. 10 (Cleaning)
Cleaning:
The FastPrep-24 instrument should be cleaned if reagents or sample
soluons spill on or inside the unit. If a sample tube leaks during a run,
the soluon will be sprayed on the bowl. Always clean any spray or spills
immediately using a damp paper towel. Always wear gloves and protecve
clothing when cleaning.
If potenally infecous agents are used in the FastPrep-24 instrument,
spills should be cleaned immediately, and appropriate decontaminaon
carried out. The FastPrep-24 instrument may not be resistant to all cleaning
regimens required for all infecous agents. Exercise appropriate cauon and
wear protecve clothing, eyewear and gloves when working with potenally
infecous samples. Contaminated units should be kept in an appropriate
biosafety level facility, and should only be maintained or serviced by
personnel trained in safe handling pracces specic to the infecous agent.
Sample Tubes
Bowl

USER MANUAL: FastPrep-24™ Classic
20
7.2 APPENDIX 2: An Explanaon of FastPrep-24
Instrument Speed Sengs
The cell disrupon process during a FastPrep-24 instrument run is caused by the
collision of matrix and sample within the FastPrep-24 instrument sample tube. The
rate of collision and energy of impact (both of which determine the eecveness
of the disrupon process) are a funcon of the FastPrep-24 instrument speed
sengs and specic gravity of the bead material used.
The FastPrep-24 instrument speed seng in meters per second refer to the
maximum vercal velocity achieved by a sample tube during reciprocang
moon. The rate of collision is proporonal to speed, while the energy of impact
is proporonal to the square of the speed. For example, a 50% increase in the
FastPrep-24 instrument speed seng increased the rate of collision by 50% and
at the same me increases the energy of impact by 125%.
The FastPrep-24 instrument has been specically designed to allow operaon
within an ideal range of parameters for disrupng membranes from a wide variety
of cell types. When used with cell-specic protocols and kits (MP Biomedicals),
cell membrane disrupon and nucleic acid yield is maximized.