Nikon Eclipse E400 User manual

Instructions

Before Using
pr
nnn
eno
con
-
i
ーーー
Thank
you
for
purchasing
this
Nikon
product.
This
instruction
manual
is
written
for the
users
of
Nikon
Microscope
ECLIPSE
E400.
To
ensure
correct
usage
read
this
manual
carefully
before
operating
the
instrument.
@
It
is
prohibited
to
reproduce
or
transmit
this
manual
in
part
or
whole
without Nikon's
expressed
permission.
©
The
contents
of
this
manual
are
subject
to
change
without
any
notice.
@
Although
every
effort
has
been
made
to
ensure
the
accuracy
of
this
manual,
if
you
note
any
points
that
are
unclear
or
incorrect,
contact
your
nearest
Nikon
representative.
—
—
Warning/Caution
Symbols
in
This
Manual
Though
Nikon
products
are
designed
to
provide
you
utmost
safety
during
use,
incorrect
usage
or
disregard
of
the
instructions
can
cause
personal
injury
or
property
damage.
For
your
safety,
read
the
instruction
manual
carefully
and
thoroughly
before
usage.
Do
not
discard
this
manual
but
keep
it
near
the
product
for
easy
reference.
Inside
this
instruction
manual,
safety
instructions
are
indicated
with
the
symbols
shown
below.
Be
sure
to
follow
the
instructions
marked
with
these
symbols
for
your
safety.
Symbol
Contents
Disregarding
instructions
marked
with
this
symbol
may
lead
to
death
or
WARNING
oo,
serious
injury.
Disregarding
instructions
marked
with
this
symbol
may
lead
to
injury
or
CAUTION
property
damage.
——
Symbol
on
the
product
Symbol
Contents
LN
Caution
for
heat
—

WARNING
ZE
Intended product use
This
microscope
should
only
be
used
for
microscopic
observation.
Do
not
use
it
for
any
other
purpose.
XE
L
Do not disassemble
Disassembly
may
cause
malfunction
and/or
electrical
shock.
Do
not
disassemble
any
parts
other
than
those
mentioned
in
this
manual.
If
you
notice
any
malfunction,
notify
your
nearest
Nikon representative.
ο
Confirm the input voltage
Make
sure
that
the
input
voltage
indicated
on
the
back
panel
of
the
microscope
is
the
same
as
your
regional
voltage.
If
not
the
same,
do
not
use
the
microscope;
instead,
notify
your
nearest
Nikon
representative.
If
the
microscope
is
used
with
the
wrong
input
voltage,
a
short
circuit
or
fire
may
result,
causing the damage of the microscope.
Power cord
To
prevent
electrical
shock,
always
turn
off
the
power
switch
(flip
it
to
the
©”
side)
before
connecting
or
disconnecting
the
power
cord.
Use
only
the
power
cord
provided
with
the
microscope.
(Refer
to
p.
24.)
Using
the
wrong
power
cord
could result in damage or fire.
$,
eat from the light source
The
lamp
becomes
extremely
hot.
To
avoid
burns,
do
not
touch
the
lamp
and
the
area
around
it
while
the
lamp
is
lit
or
for
about
thirty
minutes
after
the
lamp
has
been
turned
off.
Furthermore,
in
order
to
avoid
the
risk
of
fire,
do
not
place
fabric,
paper
or
highly
flammable
materials
(such
as
gasoline,
petroleum
benzine,
paint
thinner
or
alcohol)
near
the
lamp
while
it
is
lit
or
for
about
thirty
minutes
after
it
has
been
turned off.
The
back
of
the
microscope
also
becomes
hot
during
use.
Although
this
is
not
a
malfunction,
be
careful
not
to
touch
the back of the microscope when it is hot.

CAUTION
E Installation location
This
product
is
a
precision
optical
instrument.
Using
or
storing
the
instrument
under
unsuitable
conditions
may
damage
it
or
may
have
an
adverse
effect
on
its
precision.
The
following
conditions
should
be
kept
in
mind
when
selecting
the
installation
location.
©
Avoid
installing
in
a
place
exposed
to
direct
sunlight,
or
directly
under
room
lights
or
any
other
bright
place.
The
quality
of
the
view
through
the
microscope
deteriorates
in
bright
surroundings.
@
Install
in
a
place
with
little
dust
and
dirt.
©
Install
in
a
level
place
free
from
vibrations.
©
Install
on
a
sturdy
desk
or
table
that
is
able
to
bear
the
weight
of
the
microscope.
O
Do
not
install
in
a
warm
and
humid
location.
Otherwise,
condensation
or
mold
may
form
on
the
lenses,
degrading
performance or damaging the equipment.
Check the light source
The power supply contained in the microscope provides the power for the halogen lamp as the microscope light
source.
Although
this
power
supply
can
power
a
halogen
lamp
of
up
to
6
V-30
W,
the
specified
lamp
must
be
used.
Use of a non-specified lamp could damage the microscope.
Specified lamp: 6 V-30 W (PHILIPS 5761)
Cautions when replacing the lamp
In order to prevent burns, allow the lamp to cool (for at least thirty minutes after turning off the power) before replacing
the lamp. Furthermore, to prevent electrical shock and damage to the microscope, always turn off the power switch
(flip it to the“ ” side) and unplug the power cord before replacing the lamp.
After replacing the lamp, be sure to attach the lamp cover securely. Never use the lamp without its cover.
Cautions when operating the stage
The stage rack protrudes outward when the stage is moved.
When operating the focus knobs or condenser focus knob, be
careful not to bump your hand on the rack since the end of
the rack may injure your hand.
n em ta aray mmm oem araras rg .. arm ne -en P,

vi -
CAUTION ¿
Я Do not wet
Spilling
water
on
the
microscope
may
cause
electrical
short
circuits,
resulting
the
microscope
failure
or
abnormal
heat
generation.
If,
by
mistake,
water
or
liquid
is
spilled
on
the
microscope,
immediately
turn
off
the
power
switch
(flip
it
to
the
“ ”
side)
and
unplug
the
power
cord.
Then
use
a
dry
cloth
to
wipe
away
the
moisture.
If
any
liquid
gets
inside
of
the
microscope,
do
not
attempt
to
use
it;
instead,
contact
your
nearest
Nikon
representative.
x Weak electromagnetic waves
This
microscope
emits
weak
electromagnetic
waves.
The
accuracy
of
any
precision
electronic
equipment
may
be
adversely
affected
if
positioned
near
this
microscope.
If
this
microscope
affects
TV
or
radio
reception,
move
the
radio
or TV away from the microscope.
χει... on carrying the microscope
Securely hold onto the microscope with the base of the arm
from the back of the microscope.
Because this microscope is a precision optical device, handle
it carefully and do not subject it to strong physical shocks.
(The precision of the objective in particular may be adversely
affected by even weak physical shocks.)
In addition, do not grasp the focus knobs, the eyepiece tube,
the stage, etc., when carrying the microscope; there is a chance
that the part could come off, and the microscope could be
damaged.
When picking up the microscope or putting it down, be careful
not to bump the lamp cover on the back panel against other
things.
^7

Names of Component Parts and Operational Parts
Eyepiece tube
Eyepiece
Diopter
adjustment ring
Specimen holder
Condenser clamp
screw Eyelevel riser
Condenser focus
knob
Revolving
nosepiece
Coarse torque / tm
adjustment ring
(TORQUE)
/
я
Objective
TORQUE)
|
イイ
1
1620
コ
0
бе
ER
AVE
|
Coarse focus knob !
Fine focus knob
Brightness adjuster
(LAMP)
Stage rotation
clamp screw
Auto-photo switch
Auto-photo
voltage
ae
|
2488
selector
switch
г

Eyepiece tube
clamp screw
Eyelevel riser
clamp screw
n \ Coarse focus stopper
1 ring (CLAMP)
Fine focus knob
Condenser aperture
diaphragm ring
Condenser
Condenser centering
screw Power switch
Y-axis stage motion
Field diaphragm ring control knob
X-axis stage motion
Field
lens
control
knob
Tool holder
Rating label
AC input connector = | |
TS HE | Lamp cover
2 = 4 7
Power cord
Back panel

Proper Use Makes a Diffe
or nan ee a
h
obtained with an aperture SHE
Έλις αμ ΕΠΟ: ould be made whenever the abp
tas POS
Excessped con
details canin be süz
Proper
improper
adjustment t

yt
&
Effects
of
Condenser
Focusing
and
Centering
Center
the
condenser
so
that
the
image
of
the
field
diaphragm
appears
clearly
in
the
center
of
the
view
field.
Hf
not
centered
he
field
diaphragm
will
be
shifted
out
of
position
and
resolution
will
be
reduced.
Always
make
P.27
correctly, the center of i
these
adjustments
when
assembling
the
microscope.
Off-center
SE
|
Out
of
focus
_
Extremely
out
of
focus
ob
_
Correct
adjustment
ーー
roll
|
ian
tt
ně
Image
of
the
field
-
Image
of
the
field
Extremely
poor
diaphragm
is
off-centered
Image
of
the
field
diaphragm diaphragm
appears
fuzzy
image
quality
appears clearly m the center
of the view field
Effect
of
Color
Balancing
Filter
(NCB11)
if
specimens
are
observed
with
illumination
from
the
lamp
alone,
their
images
are
seen
with
a
yellow
tint.
Using
the
NCBI
filter
gives
the
images
a
natural
color
tone.
This
filter
is
normally
used
when
observing
specimens
by
bright
8
PAG
field microscopy.
Without using the
NCB11 filter
r
Yellowish color tone
Using the NCB11 filter
Approximates natural
daylight color
Effect
of
Lamp
Voltage
Adjustment
ht
becomes
redder.
Conversely.
if
the
voltage
is
increased,
the
d
using
the
NCB11
filter
results
in
the
most
If
the
lamp
voltage
is
decreased,
the
color
tone
of
the
hig
color
tone
of
the
light
becomes
bluer.
Pressing
the
auto-photo
switch
an
natural
color
tone.
ms
P.18
Low
lamp
voltage
z
High
lamp
voltage
-photo
switch
on
nu
e
Reddish
color
tone
Auto-photo
Swite
Bluish
color
tone
Approximates
natural
daylight
color
——%

_Contents
e
e
eee
Before Using
Names
of
Component
Parts
and
Operational
Parts
Proper
Use
Makes
a
Difference
Contents
Preparations for Microscopy
Placing
a
Specimen
on
the
Stage
Changing Magnification
Photomicrography
Locking the Focus Position
Changing
the
Torque
of
the
Coarse
Focus
Knob—
Changing the Torque of
the
Stage
Motion
Control
Knobs
Selecting a Condenser
Assembling
and
Disassembling
the
Microscope
—
Replacing
the
Lamp
Installing the Condenser
Installing the Stage
Installing the Objectives
Installing
the
Eyelevel
Riser
(If
necessary)
一
一
一
Installing the Eyepiece Tube
Installing the Eyepieces
Lens Cleaning
Keeping
the
Microscope
Clean
Storage
Regular Inspections
Troubleshooting Tables
Specifications
and
Ratings
Index
1-4
10-11
13
14
15
16
17—18
19
20
21-22
23-25
26
27
28
28
29
30
30
30
31
31
32-34
35
36

|
Preparations for Microscopy
> σα,
Q Turn on the power switch. (Flip the switch to the ” " side.) As soon as the power comes on, the switch lights.
O Place the NCB11 filter provided on the field lens and adjust the
brightness adjuster to a suitable brightness.
© Diopter and Interpupillary Distance Adjustments
These adjustments are performed to adjust the microscope to the person using it. Continued use of the microscope without
properly adjusting diopter and interpupillary distance can cause eye strain. Make sure to adjust the microscope properly whenever
you use it. Diopter adjustment compensates for differences in vision between the left and right eyes. In addition to making
observation through both eyes easier, this adjustment also reduces the defocusing when changing the objective. Perform diopter
adjustment for both eyepieces, respectively.
3-1 = Turn the diopter adjustment ring on the eyepiece to align
the edge of the diopter adjustment ring with the engraved
line. (This is the diopter adjustment "0" position.) . .
The diopter adjustment
“O” position
3-2 Turn the coarse and fine focus knobs to focus on the specimen with the 40x objective. (When it is difficult to focus on
the specimen, first focus using the 4x or 10x objective, and then switch to the 40x objective.) Since the distance
between the objective tip and the top of the cover glass is indicated as the working distance (WD) on the objective barrel,
refer to that distance when focusing on the specimen.
3-3 Move the 4x or 10x objective into the optical path. Turn
the diopter adjustment ring on the eyepiece, not the coarse
and fine focus knobs, to focus on the specimen. Do so
while peering through the right eyepiece with your right
eye and the left eyepiece with your left eye.
3-4 Adjust the spread of the binocular part to the distance
between your eyes so that the field of view for each eye
is aligned on one position when viewing with both eyes.

FP
Placing
a
Specimen
on
the
Stage
$
O
Place
the
slide
glass
containing
the
specimen
on
the
stage
with
For one
slide
glass
the
cover
glass
facing
up
and
pinch
it
in
the
specimen
holder.
Adjust
the
i!lumination
brightness
with
the
brightness
adjuster.
Color
of
the
light
changes
at
the
same
time
brightness
changes.
If
brightness
is
reduced
by
lowering
the
voltage,
the
color
tone
of
the
light
from
the
lamp
becomes
redder.
If
the
voltage
is
increased,
the
color
tone
becomes
bluer.
In
order
to
maintain
a
constant
color
tone,
adjust
brightness
by
placing
ND
filter(s)
on
the
NCBI1
filter.

0 Adjustment of condenser aperture diaphragm
Adjust the diaphragm according to the objective numerical aperture. Turning the condenser aperture diaphragm ring changes
the size of the aperture diaphragm. As the aperture diaphragm is stopped down, brightness reduces and the details of the
specimen become more difficult to see, while contrast and depth of focus increase. Conversely, as the aperture diaphragm is
opened, brightness increases and the details become easier to see, while contrast and depth of focus reduce. It is not possible to
adjust one pair of characteristics without affecting the other. Generally, a well-defined image with appropriate contrast can be
obtained with an aperture setting that is 70% to 80% of the objective numerical aperture. The numerical aperture of each
objective is indicated on the barrel of the objective.
An indication of 40x/0.75 means that the magnification is
40x and the numerical aperture is 0.75.
If the aperture diaphragm is stopped down too far, specimen
5 Magnification: 40x
Numerical aperture: 0.75
details will become difficult to see; therefore, except when
contrast is extremely low such as when viewing a nearly
transparent specimen, do not stop down the aperture to less than
60% of the numerical aperture of the objective.
®@ Adjusting the size of the aperture diaphragm according to the condenser scale
Because the condenser scale indicates the numerical aperture,
adjust the aperture diaphragm ring according to the scale.
<Example> Objective with N.A. 0.75
0.75 x 0.7 (to 0.8) = 0.525 (to 0.6)
Adjust À to this value.
@ Adjusting the size of the aperture diaphragm by using a centering telescope (sold separately)
Remove one of the eyepieces and, using a special adapter (sold separately), mount a centering telescope in its place. Turn the
aperture diaphragm ring to stop down the aperture diaphragm as far as possible. While holding down the flange of the
centering telescope, turn the eyepiece of the telescope to focus on the aperture diaphragm. Turn the aperture diaphragm ring
to adjust the size of the aperture diaphragm. Remove the centering telescope and the adapter, and then reinstall the eyepiece.
Adjustment of field diaphragm
The field diaphragm limits illumination to the area of the
specimen being viewed to prevent excessive light from entering
the view field. Turning the field diaphragm ring changes the
size of the field diaphragm. For normal observation, the size of
the diaphragm should be just outside the edge of the view field.
This adjustment can produce the image with appropriate contrast.

— Photomicrography
@
Refer
to
the
instructions
of the
photomicrographic
equipment
being
used
for
detailed
information
on
the
assembly
method
and
photomicrographic procedure.
O Selecting an eyepiece tube
:
A
trinocular
eyepiece
tube
equipped
with
a
vertical
tube
is
used.
There
are
two
types
of
trinocular
eyepiece
tubes.
Select
the
tube according to the purpose of use.
Operating
the
optical
path
selection
lever
changes
the
amount
of
light
entering
the
binocular
part
and
vertical
tube
of
the
eyepiece tube as shown below.
F eyepiece tube
T eyepiece tube
Light proportion
Lever
Binocular
Vertical
Light
proportion
position
part
tube
Lever
Binocular
Vertical
me
position
part
tube
FAD
Bino---@Mioo
:
O
~
FF RS 2 : 80
o y dg 2) 0 : 100
EMİ
poro;
3)0
:
100
EX
bo
ir;
m
BINO PHOTO
1-1 Vertical tube adapter
A photo vertical tube adapter is provided to the trinocular
eyepiece
tube
as
standard
equipment.
It
allows
a
photomicrographic
equipment
to
be
installed.
To
install
the
photo
vertical
tube
adapter,
insert
it
into
the
vertical
3
screws
tube
and
tighten
three
clamp
screws
on
the
vertical
tube
around
using
the
hexagonal
screwdriver
provided.
Replace
the
photo
tube
adapter
with
the
TV
vertical
tube
adapter
(sold
separately) in the case of using a TV camera.
1-2
Release
of
T
and
F
eyepiece
tubes
lever
clicking
“NO
CLICK”
switch
is
used
for
minimizing
slight
vibrations
produced
by
operation
of
the
optical
path
selection
lever.
This
switch
is
equipped
on
the
bottom
(installation)
surface
of
the
eyepiece
tube.
Turn
the
switch
in
the
arrow
direction
using
the
hexagonal
screwdriver
provided,
and
the
“NO
CLICK”
will
become
effective
to
remove
the
clicking
action
of
the
optical
path
selection lever.

)) !-
VE”
© Selecting color tone and brightness
The color tone of the lamp varies according to the voltage. If
the voltage is increased, the color tone of the lamp becomes bluer.
If the voltage is decreased, the color tone of the lamp becomes
tedder. Therefore, in order to obtain the best color reproduction
in color photomicrography, the lamp voltage should be kept
constant. When using daylight-type color film, the standard setup
is to use the color balancing filter (NCB11) and set the Jamp
4)
The
auto-photo
switch
is
used
to
automatically
set
the
standard
lamp
voltage.
If
the
image
on
color
film
shot
with
the
auto-photo
voltage to the standard for photomicrography.
switch
pushed
are
reddish
or
bluish,
finely
adjust
the
voltage
with
the
auto-photo
voltage
selector
switch.
The
center
position
of
the
5-level
slide
switch
is
the
standard
position.
Sliding
the
switch
forward
increases
the
bluish
tint
of
the
light,
while
sliding
the
switch
towards
the
back
increases
the
reddish
tint
of
the
light.
If
this
adjustment
is
not
enough
to
resolve
the
problem,
also
use
commercially available color compensation filters (CC filters).
© Trimming
Loosen the stage rotation clamp screw in order to rotate the stage.
Tighten the clamp screw at the desired position and secure in
position.

>)
-
Locking
the
Focus
Position
©
$
Coarse focus stopper
Once the coarse focus stopper is clamped in place, the coarse focus knob cannot be used to move the stage any higher. (Movement
of the stage by the fine focus knob is not restricted.) In effect, once the coarse focus knob is clamped in place at the focus
position, a rough focus can be attained the next time simply by turning the coarse focus knob as far as it will go.
This feature is convenient when viewing similar specimens one after another.
Using the coarse focus stopper
With the specimen in focus, turn the coarse focus stopper ring as
far as it will go (about 270°) in the arrow direction indicated on
the microscope base (i.e., clockwise direction). The coarse focus
stopper is now clamped in place.
When changing the specimen, tower the stage by turning only
the coarse focus knob.
After changing the specimen, gently raise the stage by turning
only the coarse focus knob as far as it will go.
The specimen should be roughly in focus when the stage has
been raised as far as it will go; use the fine focus knob to bring
the specimen into perfect focus.
If the coarse focus stopper is not being used, be sure to loosen
the coarse focus stopper ring as far as it will go (by turning it as
far as it will go in the opposite direction of the arrow indicated
on the microscope base).
19

, Changing the Torque of
the Coarse Focus Knob
The torque of the coarse focus knob can be adjusted.
To increase the torque, turn the coarse torque adjustment ring (TORQUE)
located behind the coarse focus knob in the arrow direction indicated on
the microscope base (i.e., the counter-clockwise direction). To reduce
the torque, turn the ring in the opposite direction of the arrow (i.e.,
clockwise direction).
Increase
the torque
Never
attempt
either
of
the
following
actions.
These
actions
will
damage
the
microscope.
@ Rotating the left and right knobs in opposite
directions at the same time.
€ Continuing to rotate the coarse focus knob after
the stage has reached the limit of its motion.
, Changing the Torque of
The torque of the X-axis and Y-axis stage motion control knobs can each be adjusted.
the Stage Motion Control Knobs
m veš
ο Adjustment of torque of Y-axis stage
motion
To tighten the tension in the Y-axis, turn Knob B in the direction
of arrow “a” while holding Knob A; to reduce the tension, turn
Knob B in the opposite direction.
© Adjustment of torque of X-axis stage
- motion
To tighten the tension in the X-axis, turn Knob C in the direction
of arrow “b” while holding Knob D; to reduce the tension, turn
Knob C in the opposite direction.

ì
Ma
- Selecting a Condenser
Object!
Achromat/
Swi
Low-
bjective
aplanat
wing-out
Achromat
Abbe
magnification
magnification
condenser
condenser
condenser
condenser
condenser
1x
κ
κ
x x
2x
x
x x
©
(Note
2)
(Note
3)
4x
x
(Note
1)
(Note
1)
10x
to
100x
©
x
©
:
Optimum
:
Appropriate
X:
Inappropriate
Note 1: The entire field of view may not be covered when a UW eyepiece is used.
Note
2:
Indoor
lighting
and
similar
light
sources
may
be
reflected
off
the
surface
of
the
condenser
lens
and
be
visible
within
the
field
of
view.
When
this
happens,
either
dim
the
indoor
lighting
or
prevent
strong
outside
light
from
entering
onto
the
stage.
Note 3: Swing out the top lens.
@
Depending
on
the
type
of
objective,
the
whole
numerical
aperture
of
the
objective
may
not
be
covered.
For
example,
when
an
objective
with
N.A.
1.4
is
used
in
combination
with
a
swing-out
condenser
or
abbe
condenser,
the
aperture
size
will
only
be
about
65%
of
the
objective's
N.A.
even
when
it
is
opened
to
its
limit.
O
For
the
universal
system
condenser
and
phase
contrast
condensers,
refer
to
each
instruction
manual.
ο Oil-immersion operation
(using
an
oil-immersion
type
objective
and
condenser)
Objectives marked “Oil” are oil-immersion type objectives.
These objectives are used with the provided immersion oil
applied between the specimen and the tip of the objective.
In order to get maximum performance from oil-immersion type
objectives with a numerical aperture of 1.0 or greater, an oil-
immersion type achromatic aplanat condenser must also be used.
Oil-immersion type condensers are used with oil applied between
the specimen and the condenser lens.
Bubbles in the oil will adversely affect the viewing of the image.
Be
careful
to
prevent
the
formation
of
air
bubbles.
To
check
for
air
bubbles,
remove
the
eyepieces,
open
the
field
diaphragm
and
the
aperture
diaphragm
as
far
as
possible,
and
look
at
the
exit
pupil
of
the
objective
within
the
eyepiece
tube.
(The
exit
pupil
will
appear
as
a
bright
circle.)
If
it
is
difficult
to
see
if
there
are
any
bubbles,
mount
a
centering
telescope
(sold
separately)
on
an
eyepiece
sleeve
by
using
an
adapter
(sold
separately).
Then,
while
turning
the
eyepiece
on
the
centering
telescope
to
change
the
focus,
look
through
the
centering telescope
for
air
bubbles.
If
there
are
bubbles
in
the
oil,
remove
them
by
one
of
the
following
methods:
Turn
the
revolving
nosepiece
slightly
to
move
the
oil-immersed
objective
back
and
forth
one
or
two
times.
(In
the
case
of
the
condenser,
gently
turn
the
condenser
focus
knob
to
move
the
condenser
up
and
down
slightly.)
©
Add
more
oil.
<>
Remove
the
oil
and
replace
it
with
new
oil.
A
©

© Use as little oil as possible (just enough to fill the space between the tip of the objective and the specimen, or between the tip
of the condenser and the specimen). If too much oil is applied, the excess will flow onto the stage and around the condenser.
© Any oil remaining on oil-immersion type objectives or on the tip of dry-type objectives has a negative effect on viewing.
After using oil, wipe all of it away and make sure that there is no oil on the tips of the other objectives. The oil on the
condenser lens should also be carefully wiped away after use.
@ Use petroleum benzine to wipe away immersion oil. Finish the cleanup by wiping with absolute alcohol (ethyl alcohol or
methyl alcohol).
© If you cannot obtain petroleum benzine, use methyl! alcohol. But it will be necessary to wipe the surfaces repeatedly (usually,
three or four times) to clean the lenses.
WARNING
@ When using petroleum benzine or absolute alcohol, please follow the instructions provided by the manufacturer.
Keep these flammable liquids away from fire or sparks.
— Precautions when using immersion oil
@ Make sure to securely tighten the cap after use. After adding oil to the container, securely tighten the base cap.
Since the base cap may become loose by itself after having been used for a long time, periodically check to make
sure it is tight. Oil may leak out if the cap is loose.
@ Do not press on the container with excessive force. This may cause the oil suddenly spray from the container.
© Wipe off any oil that collects on the outside of the container during the course of use.
O Be careful so that oil does not get in your eyes. Although the oil is not toxic, apply the first-aid measures listed
below if it should come in contact with the skin or enter the eyes.
© Contact with skin: Completely wash off with soap and water.
© Enters the eyes: Rinse thoroughly with pure water (for at least 15 minutes) and seek medical attention.
O Do not place immersion oil in a location exposed to outside light (or UV light).
© Water-immersion operation
- (using a water-immersion type objective and condenser)
O Objectives marked “WI” or “W” are water-immersion type objectives. These objectives are used with the immersion water
(distilled water or physiological saline) applied between the specimen and objective. Microscopy is performed in the same
manner as for an oil-immersion type objective.
@ Since water evaporates easily, periodically check it during observation. Applying excess water will cause it to flow onto the
stage and condenser resulting in corrosion.
@ After use, wipe any water from the tip of the objective and finish the cleanup process by wiping with absolute alcohol.
@ When the water dries and leaves a stain, apply a small amount of neutral detergent and wipe gently to remove the stain.
Finish the cleanup process by wiping with absolute alcohol.

Assembling and Disassembling
1
+ 27 the Microscope
ei
WARNING
© In order to prevent electrical shock and fire, turn off the power switch (flip to the “ ” side) during assembly.
CAUTION
>
>
@ Attach the eyepiece tube first for assembling the microscope.
@ When moving the microscope, securely grasp the arm from the back of the microscope with both hands.
@ Do not grasp the coarse focus knob, the fine focus knob, eyepiece tube or stage, etc., when carrying the microscope;
there is a chance that the part could come off, and the microscope could be damaged.
@ Be careful not to bump the lamp cover on the back of the microscope when picking it up or putting it down.
Ф Be careful not to pinch your hands or fingers when setting up the microscope.
@ Do not touch the lens surface with bare hands. Fingerprints or dirt on the lens will adversely affect the viewing of the
image.
@ This microscope is a precision optical device. Handle it carefully and do not subject it to strong physical shocks. (The
precision of the objectives in particular may be adversely affected by even weak physical shocks.)
ο Tools needed
@ Hexagonal screwdriver (1 provided)
When not using, insert it in the tool holder on the back of the microscope.
の Confirm the input voltage
全 Hikon V
MODEL EDLIESE £400
420v e
o
50/60Hz
MADE IN JAPAN
(EDA)
(AN ARNING
@ Make sure that the input voltage indicated on the back panel of the microscope is the same as your regional
voltage.
@ If the indicated voltage is different, do not use the microscope; instead, contact your nearest Nikon representative.
© If a microscope is used with the wrong input voltage, a short circuit or fire may result, and the microscope
may be damaged.