Nikon E800 User manual

Nikon

Microscope

ECLIPSE

E800

Instructions

I

Thank

you

for

purchasing

this

Nikon

product.

This

instruction

manual

is

written

for

users

of

the

Nikon

Microscope

ECLIPSE

E800.

To

ensure

corret

usage

read

this

manual

carefully

before

operating

the

instrument.

•

It

is

prohibited

to

alter

this

manual

in

part

or

whole

without

expressed

permission.

•

The

contents

of

this

manual

are

subject

to

change

without

any

notice.

•

Although

every

effort

has

been

made

to

ensure

the

accuracy

of

this

manual,

if

you

note

any

points

that

are

unclear

or

incorret,

contact

your

nearest

Nikon

representative.

•

Also

be

sure

to

read

the

instruction

manual

for

the

power

supply

that

you

are

using,

either

the

V-PS100DU,

or

the

V-PS100E.

Warning/Caution

Symbols

in

This

Manual

Though

Nikon

products

are

designed

to

provide

you

utmost

safety

during

use,

incorrect

usage

or

disregard

of

the

instructions

can

cause

personal

injury

or

property

damage.

For

your

safety,

read

the

instruction

manual

carefully

and

thoroughly

before

usage.

Do

not

discard

this

manual

but

keep

it

near

the

product

for

easy

reference.

Inside

this

instruction

manual,

safety

instructions

are

indicated

with

the

symbols

shown

be¬

low.

Be

sure

to

follow

the

instructions

marked

with

these

symbols

tor

your

safety.

Symbol

Disregarding

instructions

marked

with

this

symbol

may

lead

to

death

or

serious

injury.

Disregarding

instructions

marked

with

this

symbol

may

lead

to

injury

or

property

damage

.

CAUTION

1.

Check

the

light

source

The

power

supplies

V-PS

100DU

and

V-PS100E

provide

the

power

for

the

halogen

lamp

that

serves

as

the

microscope

light

source.

Although

these

power

supplies

can

power

a-halogen

lamp

of

up

to

12

V/

100

W,

the

specified

lamp

and

lamphouse

must

be

used.

The

equipment

could

be

damaged

if

a

non-

specified

combination

of

components

is

used.

Specified

lamphouse

:

Nikon

halogen

lamphouse

Model

C-LP

HALOGEN

12V100W

Specified

lamp

:

Halogen

lamp,

12

V-100

W

LONGLIFE

OSRAM

HLX

64623

or

PHILIPS

7724

2.

Turn

off

the

power

when

connecting

or

disconnecting

cables

or

when

replacing

the

lamp

In

order

to

prevent

electric

shock

and

damage

to

the

equipment,

always

turn

the

power

switch

on

the

power

supply

off

(flip

it

to

the

“

”

side)

and

unplug

the

power

cord

before

connecting

or

disconnecting

cables

or

replacing

the

lamp.

3.

Cautions

when

replacing

the

lamp

In

order

to

prevent

bums,

allow

the

lamp

to

cool

(for

at

least

thirty

minutes

after

turning

off

the

power)

before

replacing

the

lamp.

Furthermore,

in

order

to

prevent

electric

shock

and

damage

to

the

equipment,

always

turn

the

power

switch

on

the

power

supply

off

(flip

it

to

the

~

”

side)

and

unplug

the

power

cord

before

replacing

the

lamp.

After

replacing

the

lamp,

make

sure

that

the

lamphouse

cover

is

securely

closed.

Never

use

the

lamphouse

while

its

cover

is

open.

4.

Do

not

wet

If

the

microscope

or

the

power

supply

becomes

wet,

a

short

circuit

may

result

and

the

equipment

could

be

damaged

or

could

become

extremely

hot.

If

you

accidentally

spill

a

liquid

on

the

equipment,

immediately

turn

the

power

sw

itch

on

the

power

supply

off

(flip

it

to

the

“

”

side)

and

unplug

the

power

cord.

Then

use

a

dry'

cloth

to

wipe

away

the

moisture.

If

any

liquid

gets

inside

of

the

equipment,

do

not

attempt

to

use

it;

instead,

contact

your

nearest

Nikon

representative.

5.

Weak

electromagnetic

waves

This

microscope

emits

weak

electromagnetic

waves.

The

accuracy

of

any

precision

electronic

equipment

may

be

adversely

affected

if

positioned

near

this

microscope.

If

this

microscope

affects

TV

or

radio

reception,

move

the

radio

or

TV

away

from

the

microscope.

6.

Cautions

concerning

installation

Be

careful

not

to

pinch

your

hands

or

fingers

when

installing

the

microscope.

This

microscope

is

a

precision

optical

instrument,

and

using

or

storing

it

under

unsuitable

conditions

may

damage

it

or

may

have

an

adverse

affect

on

its

precision.

See

“Installation

Location"

on

P.27,

and

use

this

microscope

in

a

suitable

environment.

7.

Notes

on

transport

Because

this

microscope

is

a

precision

optical

device,

handle

it

carefully

and

do

not

subject

it

to

strong

physical

shocks.

(The

precision

of

the

objective

in

particular

may

be

adversely

affected

by

even

weak

physical

shocks.)

When

moving

the

microscope,

first

detach

the

lamphouse.

Then

have

at

least

two

people

carry

the

microscope,

at

least

one

on

each

side,

using

the

carrying

handles.

Be

sure

to

screw

the

carrying

handles

in

as

far

as

they

will

go

and

make

sure

that

they

are

secure.

If

the

carrying

handles

are

not

attached

securely,

they

could

come

out

or

break

while

the

microscope

is

being

carried,

creating

a

hazardous

situation.

Screw

the

carrying

hadles

into

the

upper

holes

on

the

rear

of

the

microscope.

If

you

use

the

lower

holes,

your

hands

will

be

caught

between

the

handles

and

the

table

when

you

place

the

microscope

down.

(P.27)

In

addition,

do

not

grasp

the

coarse

focus

knob,

the

fine

focus

knob,

the

eyepiece

tube,

the

stage,

etc.,

when

carrying

the

microscope;

there

is

a

chance

that

the

part

could

come

off,

and

the

equipment

could

be

damaged.

Names

of

Component

Parts

2.

Names

of

Operational

Parts

3.

Microscopic

Procedure

3

Set

the

brightness

adjuster

to

“9”.

a

This

brightness

provides

the

best

color

reproduc¬

tion.

(p.

23)

■■

Insert

filters

ND32

and

HUI

NCB11

into

the

optical

j|||l|

ND32:

Reduces

glare

in

Jlljl

the

binocular

eyepiece.

f||||

NCB11:

Improves

color

•iSra

reproduction,

(p.

21)

Lower

ND32

and

NCB11

Set

to

“9\

I

Move

the

lOx

objective

into

the

optical

path,

d

Rotate

the

revolving

^

nosepiece

until

the

lOx

3

objective

clicks

into

place.

Set

the

specimen

in

place

with

the

cover

glass

facing

Cover

glass

facing

up

la

\=r-

Select

lOx

9

i

;

3.

Microscopic

Procedure

3.

Microscopic

Procedure

Switch

to

any

desired

objective

and

view

the

specimen.

•

Re-adjust

the

focus.

•

Use

ND

filters

to

adjust

the

brightness.

•

Close

the

field

diaphragm

so

that

it

is

just

outside

the

field

of

view.

•

Close

the

condenser

aperture

diaphragm

to

about

70

to

80%

of

the

objective

numerical

aperture,

(p.

19)

■

If

a

portion

of

the

field

of

view

is

dark,

try

adjusting

the

condenser

centering

screws.

If

that

does

not

resolve

the

problem,

check

the

following

items:

•

Filter

insertion/removal

(p.

21)

•

Positioning

of

the

revolving

nosepiece

(p.

35)

•

Condenser

focusing

(p.

18)

•

Full

opening

of

the

field

and

aperturediaphragms

(p.

19,

20)

•

Optical

path

selection

for

the

eyepiece

tube

(p.

16)

•

Lamp

installation

(p.

28)

•

Revolving

nosepiece

installation

(p.

30)

•

Condenser

installation

(p.

30)

■

If

it

is

not

possible

to

focus

on

the

specimen,

check

the

following

items.

•

Mounting

of

the

specimen

(p.

35,

36)

•

Release

of

the

coarse

focus

stopper

(p.

15)

•

Thickness

of

the

cover

glass

(0.17

mm

is

standard)

(p.

35)

4.

Operation

of

Each

Part

Coarse

focus

stopper

The

coarse

focus

stopper

marks

the

stage

position

at

which

the

specimen

is

in

focus

by

restricting

the

movement

of

the

coarse

focus

knob.

(Movement

of

the

stage

by

the

fine

focus

knob

is

not

restricted.)

Once

the

coarse

focus

stopper

is

clamped

in

place,

the

coarse

focus

knob

cannot

be

used

to

move

the

stage

any

higher.

In

effect,

once

the

coarse

focus

knob

is

clamped

in

place

at

the

focus

position,

a

rough

focus

can

be

attained

the

next

time

simply

by

turning

the

coarse

focus

knob

as

far

as

it

will

go.

This

feature

is

convenient

when

viewing

similar

specimens

one

after

another.

If

the

coarse

focus

stopper

is

not

being

used,

the

coarse

focus

stopper

ring

should

be

loosened

as

far

as

it

will

go

(by

turning

it

as

far

as

it

will

go

in

the

direction

opposite

to

that

indicated

by

the

arrow

on

the

base

of

the

microscope.)

I

Using

the

coarse

focus

stopper

With

the

specimen

in

focus,

turn

the

coarse

focus

stopper

ring

as

far

as

it

will

go

in

the

direction

indicated

by

the

arrow

on

the

base

of

the

microscope

(about

270°).

The

coarse

focus

stopper

is

now

clamped

in

place.

When

changing

the

specimen,

lower

the

stage

by

turning

only

the

coarse

focus

knob.

After

changing

the

specimen,

gently

raise

the

stage

by

turning

only

the

coarse

focus

knob

as

far

as

it

will

go.

The

specimen

should

be

roughly

in

focus

when

the

stage

has

been

raised

as

far

as

it

will

go;

use

the

fine

focus

knob

to

bring

the

specimen

into

perfect

focus.

4.

Operation

of

Each

Part

Diopter

adjustment

method

1

Perform

steps

H

to

E

in

chapter

"3.

Microscopic

Procedure,”

so

that

the

specimen

is

fo¬

cused

with

the

lOx

objective.

2

Turn

the

diopter

adjustment

rings

on

the

eyepieces

and

align

the

edges

of

the

diopter

adjust¬

ment

rings

with

the

engraved

lines.

(This

is

the

diopter

adjustment

0

position.)

3

Move

the

40x

objective

into

the

optical

path.

Turn

the

coarse

and

fine

focus

knobs

to

focus

on

the

specimen.

4

Move

the

4x

or

the

lOx

objective

into

the

optical

path.

5

Turn

the

diopter

adjustment

rings

on

the

eyepieces,

not

the

coarse

and

fine

focus

knobs,

and

focus

on

the

specimen.

Do

so

while

peering

through

the

right

eyepiece

with

your

right

eye

and

the

left

eyepiece

with

your

left

eye.

6

Repeat

steps

3

to

5

twice.

I*]

Interpupillary

distance

adjustment

r--s

Before

adjusting

the

interpupillary

distance,

perform

steps

D

to

El

in

chapter

“3.

Microscopic

Pro-

:1

cedure”

so

that

the

specimen

is

focused

with

the

lOx

objective.

■

Adjust

the

interpupillary

distance

so

that

the

field

of

view

for

each

eye

is

aligned

on

one

position.

Doin°

so

will

make

observation

through

the

binocular

eyepiece

with

both

eyes

easier.

M

V'-M

4.

Operation

of

Each

Part

Condenser

aperture

diaphragm

The

aperture

diaphragm

is

important

because

it

is

related

to

the

resolution,

contrast,

depth

of

focus,

and

brightness

of

the

optical

image.

Turning

the

condenser

aperture

diaphragm

ring

changes

the

size

of

the

aperture

diaphragm.

As

the

aperture

diaphragm

is

stopped

down,

resolution

and

brightness

are

reduced

while

contrast

and

depth

of

focus

are

increased.

Conversely,

as

the

aperture

diaphragm

is

opened,

resolution

and

brightness

are

increased

while

contrast

and

depth

of

focus

are

reduced.

It

is

not

possible

to

adjust

one

pair

of

characteristics

without

affecting

the

other.

Generally,

a

satisfactory

image

with

appropriate

contrast

can

be

obtained

with

an

aperture

setting

that

is

70%

to

80%

of

the

objective

numerical

aperture.

If

the

aperture

diaphragm

is

stopped

down

too

far,

the

resolution

is

reduced;

therefore,

ex¬

cept

when

viewing

a

nearly

transparent

specimen,

we

do

not

recommend

stopping

down

the

aperture

to

less

than

60%

of

the

numerical

aperture

of

the

objective.

I

Adjusting

the

size

of

the

aperture

diaphragm

according

to

the

condenser

scale

Because

the

condenser

scale

indicates

the

numerical

aperture,

adjust

the

aperture

dia¬

phragm

ring

according

to

the

scale.

Normally,

the

index

on

the

aperture

diaphragm

ring

should

be

aligned

with

the

scale

line

corresponding

to

70%

to

80%

of

the

numerical

aperture

of

the

objective.)

Adjusting

the

size

of

the

aperture

diaphragm

by

using

a

centering

telescope

(sold

separately)

Remove

one

of

the

eyepieces

and,

using

a

special

adapter

(sold

separately),

mount

a

centering

telescope

in

its

place.

Turn

the

aperture

diaphragm

ring

to

stop

down

the

aper¬

ture

diaphragm

as

far

as

possible.

While

holding

down

the

milled

part

of

the

telescope,

turn

the

eyepiece

of

the

centering

telescope

and

focus

on

the

aperture

diaphragm

Turn

the

aperture

diaphragm

ring

to

adjust

the

size

of

the

aperture

diaphragm.

(Nor¬

mally,

the

aperture

diaphragm

should

be

adjusted

so

that

it

is

about

70%

to

80%

of

the

size

of

the

view’

field.)

Remove

the

centering

telescope

and

the

adapter,

and

then

re-install

the

eyepiece.

4.

Operation

of

Each

Part

Filters

In

this

microscope,

the

filters

indicated

below

are

housed

in

both

sides

of

the

base

of

the

microscope.

A

filter

is

inserted

into

the

optical

path

by

pressing

its

filter

insertion/removal

lever

down.

When

a

lever

is

lifted,

the

filter

is

removed

from

the

optical

path.

NCB11

(color

balancing

filter)

For

general

microscopy

and

color

photomicrography

Right

side

of

microscope

ND2

(transmission

rate:

50%)

For

brightness

adjustment

in

general

microscopy

and

photomicrography

Filter

cassette

on

left

side

of

micro¬

scope

ND8

(transmission

rate:

12.5%)

ND32

(transmission

rate:

3%)

GIF

(Green

interference

filter)

For

phase

contrast,

differential

interfer¬

ence

contrast,

and

contrast

adjustment

D

(lemon

skin)

Always

insert

in

optical

path

The

filters

in

the

filter

cassette

can

be

replaced

with

033

filters.

For

details

on

how

to

replace

the

filters,

refer

to

page

32.

Stage

Adjustment

of

torque

of

the

stage

motion

control

knobs

The

torque

of

the

X-axis

and

Y-axis

stage

motion

control

knobs

can

each

be

adjusted.

For

Y-axis

motion

To

tighen

the

tension

in

the

Y-

axis,

turn

Knob

B

in

the

direction

of

arrow

“a"

while

holding

Knob

A;

to

reduce

the

tension,

turn

Knob

B

in

the

opposite

direction.

For

X-axis

motion

To

tighten

the

tension

in

the

X-axis,

turn

Knob

C

in

the

direction

of

arrow

“b”

while

holding

Knob

D;

to

reduce

the

tension,

turn

Knob

C

in

the

opposite

direction.

a

b

TOSr

^

gums—

Knob

A

Knob

B

Knob

C

Knob

D

Preset

voltage

switch

(for

photomicrography)

The

color

temperature

of

the

lamp

varies

according

to

the

voltage.

If

the

voltage

is

high,

the

color

temperature

of

the

lamp

increases

and

the

light

becomes

bluer;

if

the

voltage

is

low,

the

color

tem¬

perature

of

the

lamp

decreases

and

the

light

becomes

redder.

Therefore,

in

order

to

obtain

the

best

color

reproduction

in

color

photomicrography,

it

is

necessary

for

the

lamp

voltage

to

be

kept

con¬

stant.

When

using

daylight-type

color

film,

the

standard

setup

is

to

use

the

color

balancing

filter

(NCB

11)

and

set

the

lamp

voltage

to

9

V.

The

preset

voltage

switch

is

used

to

automatically

set

the

standard

lamp

voltage

(9

V).

Because

the

brightness

adjuster

also

serves

as

the

preset

voltage

switch,

setting

the

brightness

adjuster

to

the

“PRESET’

1

position

automatically

sets

the

lamp

voltage

to

9

V.

Fine

adjustment

of

the

preset

voltage

If

the

final

images

on

color

film

shot

with

the

brightness

adjuster

set

to

the

“PRESET"

position

are

reddish

or

bluish,

adjust

the

preset

voltage.

Fine

adjustments

of

up

to

±

1

V

can

be

made

to

the

9

V

setting

by

using

a

minus

(-)

screwdriver

to

turn

the

rotary

switch

(the

preset

voltage

fine

adjustment

dial)

on

the

left

side

of

the

microscope

base.

When

images

are

reddish:

Increase

the

voltage.

(Doing

so

will

increase

the

lamp

color

tem¬

perature,

giving

the

light

a

bluish

tint.)

When

images

are

bluish:

Decrease

the

voltage.

(Doing

so

will

decrease

the

lamp

color

tem¬

perature,

giving

the

light

a

reddish

tint.)

If

this

adjustment

is

not

enough

to

resolve

the

problem,

also

use

color

compensation

filters

(CC

filters),

available

on

the

market.

R1

Oil-immersion

operation

■

■

(using

an

oil-immersion

type

objective

and

condenser)

23

Assembly

«/i

•Tv.

,

•

.VJ

1:1

J;;

,i

>|

-tk.

-

,,-j

•

■

•==*

•

-i

WARNING

In

order

to

prevent

electric

shock

and

fire,

turn

the

power

switch

on

the

power

supply

off

(flip

to

the

“

”

side)

during

assembly.

Also

turn

the

microscope’s

sub¬

power

switch

off.

CAUTION

•

When

moving

the

microscope,

first

detach

the

lamphouse.

Then

have

at

least

two

people

carry

the

microscope,

at

least

one

on

each

side,

using

the

carrying

handles.

•

Be

sure

to

screw

the

carrying

handles

in

as

far

as

they

will

go

and

make

sure

that

they

are

secure.

If

the

carrying

handles

are

not

attached

securely,

they

could

come

out

or

break

while

the

microscope

is

being

carried,

creating

a

hazardous

situation.

Screw

the

carrying

handles

into

the

upper

holes

on

the

rear

of

the

microscope.

If

you

use

the

lower

holes,

your

hands

will

be

caught

between

the

handles

and

the

table

when

you

set

the

microscope

down.

•

Do

not

grasp

the

coarse

focus

knob,

the

fine

foe

stage,

etc.,

when

carrying

the

microscope;

there

is

a

chance

that

the

part

could

come

off,

and

the

equipment

could

be

damaged.

•

Be

careful

not

to

pinch

your

hands

or

fingers

when

setting

up

the

microscope.

I

Tools

needed

•

Two

hexagonal

screwdrivers

(provided)

•

One

hexagonal

wrench

(provided)

•

Four

carrying

handles

(provided)

•

One

coin

(or

a

minus

(-)

screwdriver)

Assemble

each

part

in

sequence

as

numbered

in

the

diagram.

(For

details,

refer

to

pages

27

to

33.)

Viewing

will

be

adversely

affected

if

any

of

the

lenses

are

scratched

or

if

they

have

any

fingerprints

on

them.

Handle

the

lenses

carefully

during

assembly.

Because

this

microscope

is

a

precision

optical

device,

handle

it

carefully

and

do

not

subject

it

to

strong

physical

shocks.

(The

precision

of

the

objectives

in

particular

may

be

adversely

affected

by

even

weak

physical

shocks.)

25

5.

Assembly

1.

Setting

Up

the

Microscope

Q^ypiQjsj

•

When

moving

the

microscope,

have

at

least

two

people

carry

the

microscope,

at

f

least

one

on

each

side,

using

the

carrying

handles.

•

Be

sure

to

screw

the

carrying

handles

in

as

far

as

they

will

go

and

make

sure

that

they

are

secure.

If

the

carrying

handles

are

not

attached

securely,

they

could

come

out

or

break

while

the

microscope

is

being

carried,

creating

a

hazardous

situation.

Screw

the

carrying

handles

into

the

upper

holes

on

the

rear

of

the

mi¬

croscope.

If

you

use

the

lower

holes,

your

hands

will

be

caught

between

the

handles

and

the

table

when

you

set

the

microscope

down.

•

In

addition,

when

transporting

the

assembled

microscope,

do

not

grasp

the

coarse

focus

knob,

the

fine

focus

knob,

the

eyepiece

tube,

the

stage,

etc.,

when

carrying

the

microscope;

there

is

a

chance

that

the

part

could

come

off,

and

the

equipment

could

be

damaged.

•

Be

careful

not

to

pinch

your

hands

or

fingers

when

installing

the

microscope.

Installation

location

This

product

is

a

precision

optical

instrument,

and

using

or

storing

it

under

unsuitable

conditions

may

damage

it

or

may

have

an

adverse

effect

on

its

precision.

The

following

conditions

should

be

kept

in

mind

when

selecting

the

installation

location:

•

Avoid

installing

the

microscope

in

a

bright

location,

such

as

in

a

room

that

receives

direct

sunlight,

or

directly

under

room

lights.

The

quality

of

the

view

through

the

microscope

dete¬

riorates

if

there

is

a

lot

of

ambient

light.

•

Install

the

microscope

in

a

location

that

is

free

from

dust

or

dirt.

•

Install

the

microscope

on

a

flat

surface

with

little

vibration.

•

Install

the

microscope

on

a

sturdy

desk

or

table

that

is

able

to

bear

the

weight

of

the

micro¬

scope.

•

Install

the

microscope

in

a

location

where

the

temperature

will

range

from

0

to

40°C

and

where

the

humidity

is

less

than

85%.

If

the

microscope

is

installed

in

a

warm,

humid

location,

condensation

or

mold

may

form

on

the

lenses,

degrading

performance

or

damage

the

equipment.

Screw

the

carrying

handles

into

the

holes

on

both

sides

of

the

front

and

rear

(use

the

upper

holes)

of

the

microscope.

Be

sure

to

screw

all

of

the

handles

in

as

far

as

they

will

go.

Have

at

least

two

people,

with

at

least

one

on

both

sides,

carry

the

microscope

to

the

location

where

it

is

to

be

installed.

After

placing

the

microscope

in

the

desired

position,

use

a

coin

(or

minus

(-)

screwdriver)

to

remove

the

decorative

screws

on

the

rear

of

the

microscope

(in

the

lower

holes).

Remove

the

front

pair

of

carrying

handles

from

the

microscope

and

screw

them

into

the

lower

holes

on

the

rear

of

the

microscope.

Screw

the

decorative

screws

into

the

open

holes

on

the

front

of

the

27

5.

Assembly

1

Confirm

that

the

power

supply

power

switch

is

off

(i.e.,

that

it

is

flipped

to

the

“

'

”

side).

Also

confirm

that

the

sub-power

switch

on

the

microscope

is

in

the

OFF

position.

2

If

the

lamphouse

is

mounted

on

the

microscope,

use

a

hexagonal

screwdriver

to

loosen

the

lamphouse

clamp

screw

(The

screw

is

on

the

top

of

the

lamphouse

mount)

and

then

remove

the

lamphouse

from

the

microscope.

3

Use

a

hexagonal

screwdriver

to

loosen

the

clamp

screw

on

the

front

of

the

lamphouse

and

remove

the

cover.

4

While

pressing

the

lamp

clamp

lever,

push

the

lamp

into

the

socket

pin

holes

as

far

as

it

will

go.

(Do

not

touch

the

glass

portion

of

the

lamp

with

your

bare

hands.)

5

Return

the

clamp

lever

to

its

original

position.

Make

sure

that

the

lamp

is

not

crooked.

6

Close

the

cover

securely

and

tighten

the

clamp

screw.Make

sure

that

the

cover

is

fixed

in

place

and

will

not

come

off

even

when

shook

by

hand.

7

Plug

the

lamphouse

into

the

microscope

so

that

the

lamphouse

plug

fits

into

the

lamphouse

socket.

Tighten

the

lamphouse

clamp

screw

to

secure

the

lamphouse

in

place.

■

4.

Stage

Installation

Place

the

stage

with

the

bottom

facing

up.

Place

the

substage

on

top

of

the

stage.

Align

the

three

mounting

holes

on

the

stage

and

the

substage,

and

then

use

a

hexagonal

screwdriver

to

tighten

the

three

mounting

bolts

so

that

the

stage

is

securely

attached

to

the

substage.

29

5.

Assembly

Objective

Installation

Lower

the

stage

completely.

Screw

the

objectives

into

the

revolving

nosepiece

so

that

the

magnification

increases

when

the

re¬

volving

nosepiece

is

rotated

in

the

clockwise

direction,

when

looking

down

on

the

revolving

nose-

piece

from

above.

I

Note

on

removing

objectives

If

there

is

a

specimen

on

the

stage,

remove

it

first.

Lower

the

stage

completely,

and

hold

each

objective

in

both

hands

so

that

it

does

not

fall

when

you

remove

it.

|

10.

Standard

Module

Installation

\

Install

the

standard

module

by

aligning

the

two

pins

on

the

standard

module

with

the

holes

on

the

i

microscope,

and

then

use

the

hexagonal

wrench

to

tighten

the

four

bolts

with

hexagonal

holes

so

•••--!

that

the

standard

module

is

secured

in

place.

|

11.

Triocular

Eyepiece

Tube

Installation

\

Use

a

hexagonal

screwdriver

to

loosen

the

eyepiece

tube

clamp

screw.

Tilt

and

insert

the

round

:

dovetail

on

the

triocular

eyepiece

tube

to

the

round

dovetail

mount

on

the

standard

module.

Then

1

tighten

the

eyepiece

tube

clamp

screw

so

that

the

triocular

eyepiece

tube

is

secured

in

place.

5.

Assembly

Power

Supply

Connection

There

are

three

types

of

cables

that

need

to

be

connected:

the

lamp

output

cable*

the

lamp

voltage

Lamp

output

cable:

Plug

one

end

into

the

POWER

connector

on

the

rear

of

the

microscope,

and

the

other

end

into

the

LAMP

OUT

connector

on

the

rear

of

the

power

supply,

and

then

screw

on

the

locking

rings

so

that

each

connection

is

secure.

Lamp

voltage

control

cable:

Plug

one

end

into

the

CONTROL

connector

on

the

rear

of

the

microscope,

and

the

other

end

into

the

CONTROL

connector

on

the

rear

of

the

power

supply.

Power

cord:

Plug

the

socket

onto

the

AC

input

connector

on

the

rear

of

the

power

supply,

and

insert

the

plug

into

an

AC

outlet.

■

16.

Installation

of

Photomicrographic

Equipment

(Sold

Separately)

For

details

on

how

to

assemble

the

photomicrographic

equipment

and

on

how

to

take

photo¬

micrographs.

refer

to

the

instruction

manual

provided

with

the

photomicrographic

equipment.

Remove

the

cap

from

the

vertical

tube

on

the

triocular

eyepiece

tube,

and

attach

a

photo

tube.

Drop

a

PL

projection

lens

inside

the

photo

tube

(sold

separately).

Confirm

that

the

projection

lens

is

in

as

far

as

it

will

go.

t

Insert

the

main

body

of

the

photomicrographic

equipment

on

to

the

photo

tube

as

far

as

it

will

go.

With

the

finder

mount

facing

the

front,

secure

the

main

body

in

place

with

the

photographic

equip¬

ment

clamp

screws.

(If

the

photo

tube

on

the

photographic

equipment

has

a

groove

around

its

cir¬

cumference.

secure

the

equipment

in

place

with

two

small

clamp

screws.

If

there

is

no

groove

around

the

circumference

of

the

photo

tube,

secure

it

in

place

with

one

clamp

screw.)

Refer

to

the

instruction

manual

provided

with

the

photomicrographic

equipment

for

details

on

how

to

mount

a

finder,

camera

box,

etc.

H17.

Storing

the

Tools

H

When

you

finish

using

the

hexagonal

screwdriver

and

the

hexagonal

wrench,

insert

them

into

the

tool

storage

compartment

on

the

base

of

the

microscope.

Assembly

is

now

complete.

33

6.

Troubleshooting

Tables

Problem

Cause

Countermeasures

Viewing

is

poor

(too

much

or

too

little

contrast,

poor

resolution)

(The

details

cannot

be

viewed

clearly)

The

cover

glass

is

too

thick.

Use

the

specified

type

of

cover

glass

(thickness:

0.17

mm).

There

is

no

cover

glass.

There

is

no

oil

on

the

tip

of

an

oil-immersion

type

objective.

Apply

Nikon

immersion

oil.

(p.

23)

The

specified

immersion

oil

is

not

being

used.

There

are

bubbles

in

the

immersion

oil.

Remove

the

bubbles.

(p.

24)

There

is

immersion

oil

on

the

tip

of

a

dry-

type

objective.

(Especially

in

the

case

of

a

40x

objective)

Clean

the

objective.

(p.

24)

The

compensation

ring

on

an

objective

with

a

compensation

ring

has

not

been

adjusted.

Adjust

the

compensation

ring

according

to

the

cover

glass.

There

is

dirt

or

dust

on

the

lens,

condenser,

objective

or

specimen.

Clean

the

components.

(p.

38)

Uneven

focus

The

revolving

nosepiece

has

not

been

installed

properly.

Install

the

revolving

nosepiece

properly.

(p.

30)

The

revolving

nosepiece

has

not

been

rotated

until

it

has

clicked

into

place.

Turn

the

revolving

nosepiece

until

it

dicks

into

place.

(p.

9)

The

specimen

is

not

secured

in

place

on

the

stage.

Install

the

specimen

properly

on

the

specimen

holder

on

the

stage.

The

stage

has

been

installed

on

a

slant.

Install

the

stage

correctly.

(p.

29)

Image

flows

The

revolving

nosepiece

has

not

been

installed

properly.

Install

the

revolving

nosepiece

properly.

(p.

30)

The

revolving

nosepiece

has

not

been

rotated

until

it

has

clicked

into

place.

Turn

the

revolving

nosepiece

until

it

clicks

into

place.

(P-

9)

The

specimen

is

not

secured

in

place

on

the

stage.

Install

the

specimen

properly

on

the

specimen

holder

on

the

stage.

The

condenser

is

not

centered.

Center

the

condenser.

(p.

18)

The

stage

has

been

installed

on

a

slant.

Install

the

stage

correctly.

(p.

29)

Image

is

yellowish

An

NCB1

1

filter

is

not

being

used.

Use

the

NCB

11

filter.

(p.

21)

The

lamp

voltage

is

too

low.

Set

the

brightness

adjuster

to

“PRESET'

or

to

position

“9”,

and

then

adjust

the

brightness

through

the

ND

filter

combination.

(p.

23)

6.

Troubleshooting

Tables

Electrical

system

The

lamp

does

not

fight

when

the

power

is

turned

on

Cause

Countermeasure

The

power

supply

is

not

plugged

in.

Plug

the

power

cord

into

an

outlet.

(p.

32)

The

cables

between

the

microscope

and

the

power

supply

are

not

connected.

Connect

the

cables.

(p.

32)

The

sub-power

switch

is

set

to

“EPI.”

Set

the

switch

to

“DIA.”

(p.

8)

The

lamp

has

not

been

installed.

Install

the

lamp.

(p.

29)

The

lamp

is

burned

out.

Replace

the

lamp.

(p.

29)

The

specified

lamp

is

not

being

used.

Use

the

specified

lamp.

(Refer

to

the

electrical

standards

on

P.39.)

(p.

28)

The

specified

lamp

is

not

being

used.

Use

the

specified

lamp.

(Refer

to

the

electrical

standards

on

P.39.)

(p.

28)

The

lamp

voltage

control

cable

has

not

been

connected.

Connect

the

cable.

(p.

33)

The

lamp

is

near

the

end

of

its

life.

Replace

the

lamp.

(p.

29)

Inadequate

brightness

no

effect.

The

lamp

flickers;

the

brightness

The

connectors

are

not

connected

securely,

is

unstable

—-

The

lamp

is

not

plugged

into

its

socket

securely.

Secure

the

connections.

Insert

the

lamp

securely

into

its

socket.

37

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