Nikon SMZ745T User manual

Stereoscopic Zoom Microscope
SMZ745T
Instructions
M518E 09.7.NF.1

1
Thank you for purchasing a Nikon product.
This instruction manual is written for users of Nikon Stereoscopic Zoom
Microscope SMZ745T.
To ensure correct usage, read this manual carefully before operating the
product.
•No part of this manual may be reproduced or transmitted in any form
without prior written permission from Nikon.
•The contents of this manual are subject to change without notice.
•Although every effort has been made to ensure the accuracy of this
manual, errors or inconsistencies may remain. If you note any points
that are unclear or incorrect, please contact your nearest Nikon
representative.
•Some of the equipment described in this manual may not be included in
the set you have purchased.
•If you intend to use any other equipment with this product, read the
manual for that equipment too.
•If the equipment is used in a manner not specified by the manufacturer,
the protection provided by the equipment may be impaired.
Introduction

2
WARNING
CAUTION
Warning/Caution Symbols Used in this Manual
Although this product is designed and manufactured to be
completely safe during use, incorrect usage or failure to follow the
safety instructions provided may cause personal injury or property
damage. To ensure correct usage, read this manual carefully before
using the product. Do not discard this manual and keep it handy for
easy reference.
Safety instructions in this manual are marked with the following
symbols to highlight their importance. For your safety, always
follow the instructions marked with these symbols.
Symbol Description
Disregarding instructions marked with this
symbol may lead to serious injury or death.
Disregarding instructions marked with this
symbol may lead to injury or property
damage.
Safety Precautions
To ensure correct and safe operation, read this manual before using the product.

3
WARNING
1. Intended use of this product
This product is intended only for microscopy. Do not use it for any
other purposes.
2. Do not disassemble.
Disassembling this product may result in electric shock or
malfunction. Do not disassemble any part that is not indicated in
this manual. If you experience problems with the product, please
contact your nearest Nikon representative.
3. Read the instructions thoroughly.
To ensure safety, thoroughly read this manual and the manuals for
other equipment to be used with this product. In particular, be sure
to follow the warnings and cautions at the beginning of the manuals.
4. Check the input voltage
When using an illuminator, check that the input voltage displayed
on the power supply of the illuminator matches the operating
voltage. Please contact your nearest Nikon representative if the
displayed voltage does not match the operating voltage. Use of an
improperly matched illuminator may result in damage to the
equipment.
5. Cautions on the power cord
The power supply is necessary for using the halogen illuminators,
which are accessories. Follow the cautions below for the power
cord.
•To prevent electric shock, turn off the power switch on the power
supply before connecting or disconnecting the power cord.
•For the power supply TN-PSE30W A (for regions with 230 V
power only), the power code is specified. Be sure to use the
specified power cord for the power supply. Use of other power
cords may result in malfunction, overheat, or fire.
(Refer to Power Supply TN-PSE30W A (for regions with 230
V power only) on p.22.)

4
6. Cautions on heat from the light source
The lamp and the lamphouse become extremely hot when the lamp
is turned on. Follow the cautions below to prevent burns and fire.
•To avoid burns, do not touch the lamp and the lamphouse while
the lamp is on or for approximately thirty minutes after it has
been turned off.
•To avoid the risk of fire, do not place fabric, paper, or highly
flammable volatile materials (i.e. gasoline, petroleum benzine,
paint thinner, and alcohol) near the lamphouse while the lamp is
on or for about thirty minutes after it has been turned off.
7. Cautions on lamp replacement
•When replacing the lamp, wait approximately thirty minutes
after turning off the lamp, and make sure that the lamp and the
lamphouse have cooled sufficiently.
•To prevent electric shock and product damage, turn off the power
switch on the power supply before replacing the lamp.
1. Check the light source
When using an illuminator, be sure to use the specified rating only.
Use of other rating may result in damage to the equipment. Refer to
the instructions for the lamp rating.
2. Cautions on assembling the product
•Take care to avoid pinching your fingers and hands.
•Scratches and dirt (i.e. fingerprints) on the lenses will degrade
the image quality. When assembling the product, be careful not
to scratch or directly touch the lenses.
WARNING
CAUTION

5
Safety Precautions
Notes on Handling the Product
1. Handle with care.
This stereoscopic microscope is a precision optical instrument.
Handle the product with care and avoid physical shocks. Physical
shocks during transportation and operation as well as forcible
operations may result in damage to the product.
2. Installation location
Note the following conditions when installing the product.
•Install the product in a location with a temperature of 0 to +40ºC
and a relative humidity of 85% or less (no condensation).
If installed in a hot or humid location, mold may form on the
lenses or condensation may occur inside, resulting in poor
performance or damage to the product.
•Do not use the product in a location subject to direct sunlight.
•Install the product in a location with little vibration.
•Install the product in a location with little dust and dirt.
3. Cleaning the lenses
Do not allow dust, fingerprints, or any other dirt to get on the lenses.
Dirt on the lenses will degrade the image quality. If a lense becomes
dirty, clean them as described below.
•Use an air blower to blow dust away. Remove any dust by brushing
off with a soft brush or by wiping gently with a gauze, if necessary.
•Only when there are fingerprints or grease on the lense, wipe
gently with a soft, clean cotton cloth, lens tissue, or gauze
dampened with a small amount of absolute alcohol (ethyl or
methyl). Do not use the same part of the cloth more than once.
•Absolute alcohol is highly flammable. Handle with care. Do not
use near an open flame.
•When using absolute alcohol, follow the instructions provided by
the manufacturer.

6
4. Cleaning painted or plastic parts
Use of silicon cloth is recommended for cleaning painted parts,
plastic parts and printed parts. If such a part becomes excessively
dirty, wipe it gently with a gauze dampened in a mild detergent
solution. Do not use organic solvents (such as alcohol, ether, or paint
thinner). As this may result in deformation of the part or removal of
lettering.
5. Storage
•Store the product in a dry location where mold is unlikely to grow.
•Do not store the product in a location subject to direct sunlight or
high temperature and humidity.
•Put a dust-proof cover over the product to protect it from dust.
6. Regular inspections (Paid service)
Regular inspections are recommended in order to maintain the peak
performance. Please consult your nearest Nikon representative for
details about regular inspections.
Safety Precautions

7
Contents
Introduction ................................................................................... 1
Safety Precautions ........................................................................ 2
Warning/Caution Symbols Used in this Manual ..................................2
WARNING ...............................................................................3
CAUTION ................................................................................4
Notes on Handling the Product ............................................................5
INomenclature .......................................................................... 8
II Microscopy Procedures ........................................................ 10
1. Place the sample. ................................................................... 10
2. Focus on the sample. ............................................................. 10
3. Adjust the interpupillary distance. ......................................... 10
4. Adjust the diopter. .................................................................11
5. Change the zooming magnification. ......................................11
6. Change the optical path. ........................................................ 12
7. Image cannot be focused even with the zooming body
at the highest position. ...........................................................12
III Assembly ............................................................................... 13
Antistatic Feature ............................................................................... 15
IV Using Accessories ................................................................ 16
1Reticles ........................................................................................16
2Halogen Illuminators ................................................................... 17
Table 1: Total Magnification and Real Field of Binocular Tube ........23
Table 2: Total Magnification and Real Field of Vertical Tube ...........24
Table 3: Observable Sample Heights .................................................24

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INomenclature
The illustration shows the combination of SMZ745T zooming body, C-W10X
eyepieces and C-PS plain focusing stand.
The direction of DS
camera head can be
adjusted by
loosening the fixing
screw and rotating
the C mount.
Vertical tube
C mount fixing screw
Groove for mounting
accessories
Holds the fluorescent
ring illuminator and the
fiber optic ring
illuminator etc.
Outside diameter: ø60
Screw for mounting
accessories
Holds auxiliary
objectives etc.
Inside diameter screw:
M55 × 0.75
Stage plate
The black or white side can be chosen
depending on the sample. The antistatic
ESD stage plate is also available.
C mount
Nikon DS camera
head can be
attached.
Changes the light
distribution between
the vertical tube and
the right eyepiece
tube. Refer to “6.
Change the optical
path.” on p.12.
Optical path
switching lever
C mount cap
Eyepiece
10X, 15X, 20X and 30X
eyepieces are available.
Diopter ring
Refer to “ 4. Adjust the
diopter.” on p.11.
Zooming body
Outside diameter of the part
to be mounted on the arm:
ø76
Zooming body clamp screw
Fixes the zooming body to
the arm.
Arm
Sleeve
Zooming knob
Changes the
magnification of the
sample image.
Refer to “5. Change
the zooming
magnification.” on
p.11.
Focus knob
Refer to “2. Focus on
the sample.” on p.10.
Arm fixing screw
Holds the arm. The
height of the arm can
be adjusted high or
low.
Vertical slider
Hole for clip
Holds the clip for
retaining the sample.
C-PS Focusing stand
Various stands available.

9
I
Eyepiece inclination
45° from the horizontal
Screw hole for mounting an
arm for halogen illuminator
Screw holes are located on
both sides of the arm. Holds
the G-EIA flexible arm or
Epi-arm.
Screw hole for mounting
6V10W halogen illuminator
Holds the G-LS 6V10W
halogen illuminator.
Zooming knob clicks ON/OFF screw
(inside)
Refer to “ON/OFF of the
zooming knob clicks.” on p.11
Hexagonal wrench
hanger
Stores the supplied
hexagonal
wrenches.
Hexagonal wrench
(Large)
Pillar
Supplied with the
focusing stand for
changing the
height of the arm.
Ground jack
Refer to “Antistatic
Feature” on p.15.
Hexagonal wrench
(Small)
Supplied with the
zooming body for
switching ON/OFF
the zooming knob
clicks.

10
II Microscopy Procedures
1Place the sample.
Place the sample in the optical path on the stage plate of the stand.
The black or white side can be chosen depending on the sample.
Use the stage clips to hold the sample, if necessary.
2Focus on the sample.
Turning the left and right focus knobs to the same direction will raise or
lower the arm (on which the zooming body is mounted) and focus on the
sample.
■Working distance
The distance between the focus plane and the bottom surface of the
zooming body is called “the working distance”. Since the working
distance of the SMZ745T is 115 mm, the focusing will become easier if
you set the zooming body at the position where its bottom surface is 115
mm apart from the sample surface. Refer to Table 1 (p.23) for changing
the working distance when the auxiliary objectives are attached.
■The torque of the focus knob
If the torque of the focus knob is too light, the
zooming body falls down by its own weight.
Adjust the torque to an appropriate weight.
(Refer to “7. Adjust the torque of the focus
knob.” on p.14.)
3Adjust the interpupillary distance.
This adjustment is for adjusting the distance
between both eyes of the observer.
Adjust the interpupillary distance so that the
fields of view for each eye are merged into
one. Move while holding each sleeve with
both hands.
This adjustment is required for each observer
since individual interpupillary distance vary.
•If the microscope has not been assembled, first refer to Chapter III, “Assembly.”
The arm moves down by turning
the focus knob towards the
observer.

11
4Adjust the diopter.
This adjustment is for adjusting the eyesight of the observer.
1Turn the diopter rings on both eyepieces to set them at the 0 position
(match the 0 line with the index line).
2Turn the zooming knob to 5X. Focus on the sample using the focus
knob. (Refer to “2. Focus on the sample.” on p.10.)
3Turn the zooming knob to 0.67X. Look into the left eyepiece with
the left eye, and focus on the sample using the diopter ring on the
left eyepiece. Then, look into the right eyepiece with your right eye
and focus on the sample using the diopter ring on the right eyepiece.
4Repeat steps 2and 3until the image is kept focused even though the
zooming magnification is changed. This adjustment ensures the
sharp image throughout the zooming range.
This adjustment should be performed each time the observer is changed
since individual eyesights vary.
5Change the zooming magnification.
The magnification of the sample image will change by turning the left and
right zooming knobs of the zooming body. Choose the desired
magnification.
■Total magnification
The right zooming knob from the viewing side has the indication of the
zooming magnification. Total magnification can be calculated by
multiplying the eyepiece magnification by the zooming magnification.
Note) When the auxiliary objective is attached,
multiply its magnification as well.
■ON/OFF of the zooming knob clicks
The clicks on the ON/OFF of the zooming
knob are available.
1Remove the small rubber cap from the
rear of the zooming body.
2Insert the supplied hexagonal wrench
(small) into the screw hole on the rear of
the zooming body.
II
ON
OFF
Zooming
knob

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II
Microscopy Procedures
Auxiliary
adapter
0.5X Auxiliary
objective
3ON/OFF of the zooming knob clicks are adjustable by turning the
inside screw.
4Return the small rubber cap to its original position.
6Change the optical path.
The light distribution between the vertical
tube and the right eyepiece tube can be
switched with the optical path switching lever.
For binocular observation, move the lever
foward. When Nikon DS camera head is
mounted to the C mount of the zooming body
for taking microphotographs, move the lever
backward.
Please refer to the instruction of the camera
head for the details of the Nikon DS Camera
Head operation.
☞• Refer to Table 2 (p.24) for Total Magnification and Real Field of
Vertical Tube.
7Image cannot be focused even with the
zooming body at the highest position.
For using the 0.5X auxiliary objective or for
observing a tall sample, you may not be able
to focus on the sample even if turning the
focus knob to raise the zooming body to its
highest position.
In this case, use an optional C-ER auxiliary
adapter.
Note) Attach the auxiliary objective first to
the zooming body, then mount the
zooming body on the auxiliary adapter.
☞• Refer to Table 3 (p.24) for Observable Sample Heights.
Right eyepiece : Vertical tube
=100 : 0
Right eyepiece : Vertical tube
=100 : 0
Optical path
switching lever

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III Assembly
1Place the stand on the level surface.
2Mount the stage plate.
Press the stage plate into the stand base while
pushing it against the rim in the direction
shown by the arrow in the illustration.
3The stand arm can be lowered.
(For not lowering the arm, jump to
step 4.)
Use the hexagonal wrench (large, supplied
with the focusing stand) to loosen the arm
fixing screw. Reattach the arm using the
screw hole at the lower side of the vertical
slider. The arm will become 55 mm lower
than its original position. Before tightening
the screw, make sure that the two pins on the
arm are inserted into the grooves in the
vertical slider.
4Mount the zooming body.
Lightly tighten the zooming body clamp
screw to hold the zooming body on the arm.
Note) Do not overly tighten the zooming body
clamp screw since it may cause
damage to the product.
Groove
Pin

14
5Insert the eyepieces into the eyepiece sleeves.
Be sure that it is inserted all the way to the end of the sleeve.
Note) When inserting the 10X eyepiece, assure that it reaches the end of
the sleeve, because the rubber cover of the 10X eyepiece will
obstruct the view of the sleeve end. When inserting, hold the rubber
cover instead of the diopter ring. If not, it may result in the damage
to the product.
6Attach the camera head (optional).
Remove the C mount cap from the zooming
body.
Mount the C mount adapter to DS camera
head first. Then, attach the DS camera head to
the C mount of the zooming body.
The direction of DS camera head can be
adjusted by loosening the fixing screw and
rotating the C mount.
7Adjust the torque of the focus knob.
Adjust the torque of the focus knob so as not
to fall down the zooming body by its own
weight.
8Storing the tools
The hexagonal wrenches can be stored at the rear of the focusing stand.
Refer to the figure on p.9.
Increase the torque
(To reduce the torque,
turn the knob in the direction
opposite to the arrow.)

15
III
Antistatic Feature
The SMZ745T, the C-PS/C-PSC plain focusing stand and the C-W10X
eyepieces are antistatic. You can enjoy this feature when observing the sample
that cannot stand much static. When using the microscope on this purpose,
change the stage plate to antistatic ESD stage plate and ground the microscope
through the ground jack at the rear of the plain focusing stand. The φ 4 mm
grounding terminal can be inserted into the ground jack.
The following accessories are also antistatic.
•C-FPS Fluorescence Attachment
•Auxiliary Objectives (all types)
•C-FMB Focusing Mount B
•G-US1 and G-US2 Universal Table Stands
Assembly

16
IV Using Accessories
1Reticles
Your reticles can be attached to the eyepiece.
Remove the field ring (or lens room of the
20X and 30X) from the eyepiece, then attach
the reticle with its pattern surface facing down
to the eyepiece, and reattach the field ring (or
lens room).
☞• Refer to Table 1 (p.23) for the size of
the reticle which can be attached.
Note) The 20X and 30X eyepieces adopt the
internal focusing system, and the
primary image has the magnification.
Use the reticle with 1.3X pattern for
20X eyepiece, and with 1.4X pattern
for 30X eyepiece.
An optional reticle with cross hairs or
scaled cross hairs is available for 10X
eyepiece.
Optional
reticle
Reticle
Pattern
surface
Field ring

17
2Halogen Illuminators
There are two illuminators available: the G-LS of 6V10W and the
C-DSLS of 6V20W.
Lamphouse
Lamp socket
(Removable
back and forth)
Plug
Mounting screw
PlugLamphouse Lamp socket
Filter cap
ø33 mm filter
attachable
Holder and Epi arm
mounting screw hole
IV
G-LS
Lamp Socket A (6V10W)
C-DSLS
Illuminator A (6V20W)
1Lamp
• When replacing the lamp, wait approximately thirty minutes
after turning off the lamp, and make sure that the lamp and the
lamphouse have cooled sufficiently.
• To prevent electric shock and product damage, turn off the
power switch on the power supply before replacing the lamp.
WARNING

18
Pull the lamp socket from the lamphouse and insert the lamp straight into
the socket to the end. Use halogen lamps of the specified rating only.
Then, attach the lamp socket to the lamphouse.
Note) Do not touch the glass part of the lamp with bare hands as it may
burn and stick to your skin, possibly reducing its level of light
output or causing damage to the lamp. When replacing a lamp,
handle it with a cloth or through its packaging until the
replacement is completed. If you get fingerprints on the lamp, wipe
gently with a clean cloth dampened with alcohol.
Socket
Lamp
(GE 787 6V-10W)
Socket Small protrusion
Holder mounting
screw hole
Light
shielding tube
Lamp
(KLS JCR/M
6V20W-2)
Confirmation
window
G-LS
C-DSLS