Nikon SC User manual

Table of contents

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Nikon

MICROSCOPE

MODEL

SC

Instructions

NIPPON

KOGAKU

K.K.

CAUTIONS

IN

HANDLING

CARE

AND

MAINTENANCE

^

Red-colored

lock

screw

Before

handling

the

microscope,

be

sure

to

remove

the

red-colored

lock

screw.

Exchanging

the

lamp

bulb

and

fuse

Before

replacing

the

lamp

bulb

or

fuse,

be

sure

to

turn

the

power

switch

to

OFF

and

pull

out

the

power

source

cord

from

the

socket.

^

Place

for

using

Avoid

the

use

of

microscope

in

a

dusty

place,

where

it

is

subject

to

vibrations

or

exposed

to

high

temperatures,

moisture

or

direct

sunlight.

^

Dirt

on

the

lens

Do

not

leave

dust,

dirt

or

finger

marks

on

the

lens

surfaces.

They

will

prevent

you

from

clear

observa¬

tion

of

the

specimen

image.

e

Avoid

sharp

knocks!

Handle

the

microscope

gently,

taking

care

to

avoid

sharp

knocks.

Use

SC

type

objectives

&

eyepieces

For

the

SC

microscope,

use

no

other

objec¬

tives

and

eyepieces

that

of

SC

type.

^

Cleaning

the

lenses

To

clean

the

lens

surfaces,

remove

dust

using

a

soft

hair

brush

or

gauze.

Only

for

removing

finger

marks

or

grease,

should

soft

cotton

cloth,

lens

tissue

or

gauze

light¬

ly

moistened

with

absolute

alcohol

(eth¬

anol

or

methanol)

be

used.

For

cleaning

the

objectives

and

immersion

oil

only

use

xylene.

Observe

sufficient

caution

in

handling

alcohol

and

xylene.

0

Cleaning

the

painted

surfaces

Avoid

the

use

of

any

organic

solvent

(For

example;

thinner,

xylene,

ether,

alcohol

etc.)

for

cleaning

the

painted

surfaces

and

plastic

parts

of

the

instrument.

0

Never

attempt

to

dismantle!

Never

attempt

to

dismantle

the

instrument

so

as

to

avoid

the

possibility

of

impairing

the

operational

efficiency

and

accuracy.

Q

When

not

in

use

When

not

in

use

cover

the

instrument

with

the

accessory

vinyl

cover

or

put

in

the

case

(available

on

order)

and

store

it

in

a

place

free

from

moisture

and

fungus.

Periodical

checking

To

maintain

the

performance

of

the

instru¬

ment,

we

recommend

the

customers

to

check

the

instrument

periodically.

For

details,

contact

our

dealers.

CONTENTS

I.

NOMENCLATURE

AND

FUNCTION.

4

II.

ASSEMBLY.

g

1.

Attaching

the

eyepiece

tube.

g

2.

Attaching

the

eyepiece.

g

3.

Attaching

the

mechanical

stage.

0

4.

Attaching

the

objectives.

0

5.

Attaching

the

condenser.

y

6

.

Attaching

the

lamp

bulb.

7

7.

Attaching

the

lamp

bulb

for

simplified

illumination

.

,

7

III.

OBSERVATION.

0

1.

Setting

the

specimen

in

position.

g

2.

Adjusting

the

illumination.

g

3.

Adjusting

interpupillary

distance.

g

4.

Adjusting

for

eyesight

difference.

g

5.

Coarse

focusing.

,

,

g

6

.

Fine

focusing.

g

7.

Working

distance

of

objectives.

g

8

.

Vertical

movement

of

condenser.

1

q

9.

Condenser

diaphragm.

.

|q

10.

Oil-immersion

observation.

11.

Moving

the

glass

si

ide.

1

0

IV.

OPTICAL

FEATURES

OF

SC

MICROSCOPE.H

V.

TROUBLE

SHOOTING

CHART.

1

2

1.

Optical

system

.

12

2

Manipulation

system

.

13

3.

Electrical

system

.

14

ELECTRIC

SPECIFICATIONS.

15

I

.

NOMENCLATURE

AND

FUNCTION

The

descriptions

in

this

instruction

manual,

for

the

most

part,

cover

the

use

of

the

binocular

eyepiece

lube

microscope.

They

are

applicable,

except

where

not

specified,

to

the

monocular

eyepiece

type

as

well.

Coarse

focus

knob

Power

cord

SC

eyepiece

Diopter

ring

_

Used

for

eyesight

adjustment.

Binocular

eyepiece

tube

Arm

One

rotation

moves

the

stage

about

24mm.

Fine

focus

knob

Diaphragm

control

ring

Condenser

focus

knob

Microscope

base

Eyepiece

sleeve

Hold

the

sleeves,

when

adjusting

interpupillary

distance.

Revolving

nosepiece

Accepts

4

objectives-

SC

objective

Range'.

2.2mm

One

rotation

moves

the

stage

about

0.2mm.

Power

switch

and

brightness

control

dial

More

brightness

is

obtained

by

turning

toward

the

thicker

end

of

the

arrow.

Condenser

Fig.

1

4

Slide

holder

Mechanical

stage

Range:

38

x

76mm

Filter

holder

For

filters

of

33mm

in

diarrwter.

Filter

receptacle

_

For

filters

of

45mm

in

diameter.

Stage

Y-axis

travel

knob

Stage

X-axis

travel

knob

Condenser

clamp

screw

For

locking

the

condenser.

For

tightening,

use

the

tool.

Eyepiece

tube

clamp

screw

Lightly

released,

the

tube

is

rotatable.

Fig.

2

tube

Stage

clip

For

holding

the

specimen

in

position

illuminator

Fig.

3

5

II.

ASSEMBLY

Before

handling

the

microscope,

remove

the

red-

colored

lock

screw.

(Fig.

4)

1.

Attaching

the

eyepiece

tube

(Fig.

5)

i;

Slacken

the

eyepiece

tube

clamp

screw

suf¬

ficiently.

2)

Tilting

the

eyepiece

tube

as

^own

in

Fig.

5,

seat

it

in

a

horizontal

position.

3)

Fasten

up

the

clamp

screw

securely.

2.

Attaching

the

eyepiece

(Fig.

6)

1)

Remove

the

dust

caps

from

the

tubes.

2

)

Screw

in

the

eyepieces.

3;

To

avoid

accidental

release,

screw

the

eye¬

piece

in

firmly,

using

a

rubber

sheet

or

the

like

to

hold

the

eyepiece.

3.

Attaching

the

mechanical

stage

(Fig.

7)

1}

Place

the

mechanical

stage

on

the

stage

mount

so

that

the

travel

knobs

are

on

the

right

side

of

an

operator

facing

the

stage.

2)

Fasten

the

stage

from

the

bottom

with

the

two

4mm

hexagonal

hole

bolts,

using

tool

A.

4.

Attaching

the

objectives

(Fig.

8)

1)

Screw

the

objectives

into

the

revolving

nose-

piece

in

such

order

that,

as

the

nosepiece

is

rotated

clockwise,

an

objective

of

higher

magnifying

power

enters

the

optical

path.

2)

For

avoiding

accidental

release,

it

is

possible

to

lock

them

tightly

using

tool

C,

Eyepiece

tube

clamp

screw

Tool

C

Mechanical

stage

Travel

knobs

Revolvmg

nosepiece

6

5.

Attaching

the

condenser

1}

Place

the

blue

filter

into

the

filter

holder.

(Fig.

9)

21

Slide

the

condenser

into

its

mount

as

far

as

it

will

go

in

position

where

the

aperture

number

plate

turns

toward

the

operator.

3)

Tighten

the

condenser

clamp

screw,

using

the

tool

B.

(Fig.

10)

Note:

The

tools

A,

B

and

C

(Fig.

11)

are

not

included

in

the

microscope

set.

If

necessary,

purchase

them

independ¬

ently.

6

.

Attaching

the

lamp

bulb

The

microscope

is

delivered

with

its

lamp

bulb

(100V,

115/125

or

220/240V-30W)

attached.

If

it

is

necessary

to

replace

the

bulb

at

a

later

time,

proceed

as

below;

Be

sure

to

remove

the

power

cord

from

the

socket,

beforehand.

1)

Lay

the

instrument

down

carefully

to

permit

unscrewing

the

set

screw

found

on

the

bottom

lid

of

the

microscope.

A

screw

driver

or

coin

may

be

used.

2)

Sliding

the

bottom

lid

to

the

side

of

the

set

screw,

remove

it.

The

lamp

bulb

will

then

be

accessible.

(Fig.

12)

3)

To

attach

the

bulb,

pushing

the

lamp

bulb

with

its

silver

surface

turned

to

the

side

of

the

bottom

lid

insert

the

bulb

into

the

socket.

(Fig.

13)

Note:

Finger

marks

left

on

the

lamp

bulb

sur¬

face

will

decrease

the

brightness

of

image.

In

handling

the

bulb,

use

a

cloth

or

wear

gloves.

4)

Replace

the

bottom

lid.

7.

Attaching

the

lamp

bulb

for

simplified

il¬

lumination

(Fig,

14)

This

lamp

bulb

(110/120V-10W

or

220/240V

-

12W)

is

to

be

used

exclusively

for

the

monoc¬

ular

eyepiece

tube

type

of

microscope.

1)

Be

sure

to

remove

the

power

cord

from

the

socket.

2)

Remove

the

lamp

socket

from

the

instru¬

ment

by

turning.

3)

Screw

the

lamp

bulb

into

the

socket

as

far

as

it

will

go.

4)

Reattach

the

socket.

Condenser

clamp

screw

Condenser

Bottom

lid

Aperture

number

plate

Fig.

to

1

A

B

c

1

1

Tools

available

on

order

Fig.

12

Silver

surface

of

the

lamp

bulb

Fig.

14

7

III.

OBSERVATION

1.

Setting

the

specimen

in

position

1)

Place

the

specimen

on

the

stage

holding

in

position

by

means

of

the

slide

holder.

{Fig.

15)

2)

When

not

using

the

mechanical

stage,

hold

the

specimen

securely

with

the

two

stage

clips.

(Fig.

16)

2.

Adjusting

the

illumination

1)

Insert

the

power

cord

into

the

socket.

2)

Raise

the

condenser

to

its

upper

limit,

and

fully

open

the

condenser

diaphragm.

3)

Turn

the

power

switch

&

brightness

control

dial

to

light

the

lamp,

(Fig.

17)

Brightness

is

increased

by

turning

toward

the

thicker

end

of

the

arrow,

and

decreased

to¬

ward

the

thinner.

4)

Rotate

the

revolving

nosepiece

to

swing

the

4X

or

10X

objective

into

the

optical

path.

At

this

time,

make

sure

that

the

objective

clicks

properly

into

position,

3.

Adjusting

interpupillary

distance

(Fig.

18)

When

using

a

binocular

eyepiece

tube,

adjust

the

interpupillary

distance,

so

that

both

the

right

and

left

viewfields

become

one.

4.

Adjusting

for

eyesight

difference

(Fig.

19)

1)

First,

viewing

into

the

right

eyepiece

with

the

lOX

objective

in

position,

manipulate

the

coarse

and

fine

focus

knobs

to

bring

the

specimen

into

focus.

2}

Then,

viewing

into

the

left

eyepiece,

turn

the

diopter

ring

to

bring

the

image

into

sharp

focus,

without

manipulating

the

focus

knobs.

0

index

is

put

on

the

diopter

ring.

Stage

clips

Fig.

16

8

5.

Coarse

focusing

1)

The

coarse

focus

knob

is

to

be

used

for

coarse

focusing

and

for

fine

focusing

with

the

4X

objective.

2)

It

can

also

be

used

for

lowering

the

stage

convenience

when

replacing

a

specimen,

removing

immersion

oil.

etc.

3)

The

relation

between

the

direction

of

rota¬

tion

of

the

coarse

focus

knob

and

that

of

vertical

movement

of

the

stage

is

as

indicat¬

ed

by

the

arrows

in

Fig.

20-A.

B.

6

.

Fine

Focusing

1)

The

fine

focus

knob

is

to

be

used

for

fine

focusing

with

the

10X

or

higher

power

objectives.

2

)

It

is

also

used

when

exchanging

objectives

or

focusing

on

each

successive

layer

of

a

thick

specimen,

3)

The

relation

between

the

direction

of

rota¬

tion

of

the

fine

focus

knob

and

that

of

ver¬

tical

movement

of

the

stage

is

the

same

as

for

the

coarse

focus

knob.

{Fig.

20-A.

B)

4)

The

range

of

the

fine

focus

knob

is

2.2mm.

The

white

line

on

the

fine

focus

knob

shows

the

center

of

the

fine

focusing

range.

It

is

necessary

beforehand

to

position

the

white

line

on

the

fine

focus

knob

opposite

the

end

of

the

coarse

focus

knob.

(Fig.

21)

7.

Working

distance

of

objectives

1)

"Working

distance"

is

the

clearance

between

the

front

of

objective

and

the

upper

surface

of

the

coverglass

when

the

image

of

the

specimen

is

in

sharp

focus.

In

this

microscope

it

is

as

follows

for

each

objective:

Objective

Working

distance

4XSC.28mm

lOXSC.8mm

40XSC.0.73mm

lOOXSC.0.16mm

2}

As

shown

above,

the

working

distances

of

the

40x

and

100X

objectives

are

very

small,

incurring

the

danger

of

pressing

on

the

specimen.

These

two

objectives,

therefore,

are

specifically

equipped

with

a

safety

de¬

vice,

which

permits

rising

of

the

front

of

objective,

at

the

moment

it

touches

the

specimen.

Fig.

21

9

3)

T

o

proceed

to

focusing,

first

raise

the

stage

to

the

approximate

working

distance

of

the

objective

being

used.

Then,

viewing

into

the

microscope,

lower

the

stage

sicwviy,

until

the

image

is

brought

into

sharp

focus,

8

.

Vertical

movement

of

condenser

1)

For

general

use,

raise

the

condenser

to

its

upper

limit.

2

)

When

examining

non-stained

specimens,

it

is

preferable

to

lower

the

condenser

to

such

an

extent

that

a

sufficient

image

contrast

is

ob¬

tained,

rather

than

to

close

the

condenser

diaphragm,

because

it

has

an

advantage

that

the

contrast

and

brightness

do

not

change

so

abruptly

and

greatly.

9.

Condenser

diaphragm

The

condenser

diaphragm

is

intended

to

adjust

its

numerical

aperture

(N.A.)

and

not

the

bright¬

ness

of

illumination.

1)

When

closed,

the

condenser

diaphragm

will

increase

the

image

contrast

but

decrease

the

resolving

power.

If

excessively

closed,

it

causes

fringes

visible

at

the

edge

of

image

details.

2)

Opening

of

the

diaphragm

is

continuously

adjustable

within

a

range

from

2.2mm

to

30

mm

in

diameter.

For

general

use,

it

should

be

stopped

down

to

70~80%

of

the

nu¬

merical

aperture

of

the

objective

to

achieve

an

appropriate

image

contrast.

For

adjust¬

ment

of

the

diaphragm,

when

observing,

turn

the

diaphragm

control

ring

so

as

to

obtain

an

image

quality

suitable

for

the

specimen.

10.

O

il-immersion

observation

(Fig,

21)

When

using

the

100X

objective

marked

with

a

black

ring,

the

specimen

should

be

immersed

in

oil

by

applying

Nikon

immersion

oil

between

the

coverglass

and

the

front

lens

of

the

objective.

Fig.

22

1)

Using

a

10X

or

40X

objective

focus

on

the

specimen.

Bring

the

desired

detail

in

the

specimen

to

the

center

of

the

field

of

view.

2)

Turning

the

revolving

nosepiece,

place

the

100

X

objective

in

a

position

where

it

is

con¬

venient

to

apply

oil.

Then,

tilt

the

oil

con¬

tainer,

until

oil

enters

the

nozzle.

Depressing

the

container,

attach

an

appropriate

amount

of

oil

onto

the

coverglass.

(Fig.

221

The

oil

may

take

in

air

bubbles.

If

any

air

bubble

is

seen,

clear

off

the

oil

using

lens

tissue,

and

apply

the

oil

once

again.

31

Thereafter,

keeping

the

container

depressed,

apply

the

oil

on

the

top

end

of

the

nozzle

to

that

of

the

objective.

Then,

rotate

the

100X

objective

into

the

optical

path.

4)

If

air

bubbles

enter

the

oil

layer,

a

poor

image

will

result.

To

remove

the

air

bubbles,

turn

the

revolving

nosepiece

several

times

laterally

or

apply

an

additional

drop

of

oil.

5)

If

a

higher

resolving

power

is

desired,

also

apply

oil

between

the

bottom

of

the

glass

slide

and

the

condenser

top

lens.

Note:

For

cleaning

the

oil,

after

finishing

oil-

immersion

observation,

wipe

it

off

thoroughly

with

lens

tissue.

11.

M

oving

the

glass

slide

The

lateral

travel

range

of

the

glass

slide

by

means

of

the

smaller

knob

on

the

mechanical

stage

is

76mm

(2.99

in),

and

the

back

and

forth

travel

range

by

means

of

the

larger

knob,

38mm

(1.5

in).

10