Nippon Genetics FastGene FAS-Digi PRO User manual

Gel Documentation <GP-07LED>
Product
FastGene®FAS-Digi PRO
Cat. No.
GP-07LED
Category
Gel Documentation System
Version 1.3
July/2019

Manual <GP-07LED>

Manual <GP-07LED>
Content
General Information 5
SAFETY INFORMATION 5
REGULATORY NOTICE 5
WARRANTY 5
IMPORTANT NOTICE 5
Specification 6
CAMERA 6
CONTROL 6
DARKBOX 6
ILLUMINATOR 6
UNIT 6
Nomenclature 7
Installation guide –Setting up the FAS-Digi PRO 10
ASSEMBLING THE CAMERA HOLDER 10
CONNECTING THE CABLES 13
CONNECTING THE CAMERA TO A PC 14
Installation, Operation and Performance Qualification 20
COMPUTER MINIMAL REQUIREMENTS 22
INSTALLATION QUALIFICATION 22
OPERATION QUALIFICATION 22
PERFORMANCE QUALIFICATION 23
Operating the System 26
LOG IN 26
MAIN MENU 26
SELECTING THE APERTURE 27
SELECTING THE EXPOSURE TIME 27
SELECTING THE SENSITIVITY OF THE SENSOR 27
TAKING IMAGES WITH DIFFERENT EXPOSURE TIMES 27
FOCUSING THE IMAGE 27
CHANGE THE IMAGE SETTINGS 28
PRINTING THE LIVE VIEW 28
DISPLAYING THE RECORDING INFORMATION 28
CAPTURING THE IMAGE 28
Excising DNA bands 29
REMOVING THE FILTER SHIELD FROM THE DOOR 29
ADDING THE FILTER SHIELD TO THE CUTTING POSITION 29
EXCISE THE DNA BAND 29
Taking images using a white light source 30
Contact Details 31

Manual <GP-07LED>

Manual <GP-07LED>
General Information
SAFETY INFORMATION
Please read carefully the following notes to be able to
properly use the FastGene®FAS-Digi PRO imaging
system.
Please wear the appropriate personal protective
equipment.
Please read the safety precautions stated in the
Canon 200D operating manual.
Using FastGene®Blue/Green LED XL-
Transilluminator instrument will normally not injure
eyes, skins, and samples. However, prolonged
exposure of human naked eyes with light irradiation
in the blue spectrum may increase the probability of
suffering from retina diseases. Therefore, we
recommend using the amber filter included in the
box, when handling the transilluminator while the
FastGene®FAS-Digi PRO box is open.
Operate the FAS-Digi PRO only if it is connected to
a safety socket.
Place the unit on a level surface with minimal chance
of dropping.
REGULATORY NOTICE
IMPORTANT: This Nippon Genetics Europe instrument is
designed and certified to meet safety standards and EMC
regulations. Certified products are safe to use when
operated in accordance with the instruction manual. This
instrument must not be modified or altered in any way.
Alteration of this instrument will:
•Void the manufacturer’s warranty
•Void the safety and EMC certification
•Create a potential safety hazard
Nippon Genetics Europe is not responsible for any injury
or damage caused by the use of this instrument for
purposes other than those for which it is intended, or by
modifications of the instrument not performed by
Nippon Genetics Europe or an authorized agent.
WARRANTY
The FastGene®Blue/Green LED XL-Transilluminator and
the FastGene®FAS-Digi PRO is warranted against defects
in materials and workmanship for 1 year. In case of any
defects occurring in the instrument or accessories during
this warranty period, Nippon Genetics Europe will repair
or replace the defective parts at its discretion without
charge.
The following defects, however, are specifically excluded:
•Defects caused by improper operation.
•Repair or modification done by anyone other
than Nippon Genetics Europe or an authorized
agent.
•Damage caused by substituting alternative
parts.
•Use of fittings or spare parts supplied by anyone
other than Nippon Genetics Europe.
•Damage caused by accident or misuse.
•Damage caused by disaster.
•Corrosion caused by improper solvent or
sample.
For any inquiry or request for repair service, contact
Nippon Genetics Europe or your local distributer. Please
send a message containing information about the model
and serial number of your instrument.
IMPORTANT NOTICE
Please, read the installation instruction carefully before
installing the FastGene®Blue/Green LED XL-
Transilluminator and the FastGene®FAS-Digi PRO
Imaging System. This instrument is intended for clinical
and research laboratory use with DNA gel activation and
it must be operated only by specialized personnel aware
of the potential risks associated with the chemical and
biological agents normally used with this unit and the
health risks associated with blue light radiation. Please
wear the amber goggle or use the amber shield for your
own protection!
Please make sure to connect the instrument only to a
protective current!

Manual <GP-07LED>
Specification
Camera
Camera type
Canon 200D, 24 MPixel (Resolution: 6000x4000), APS-C
Sensor DIGIC 7 Processor, WiFi enabled
Image Sensor
APS-C sized CMOS Sensor
Resolution/Image Size
6000 x 4000 pixel
Bit Output Format
8-bit
Aperture
F/4-5.6
Lens
18-55 mm zoom Lens, manual
Exposure time
0.00025 to 30 seconds
Filter
FastGene®amber filter attached by magnets
Control
Control Software
NIPPON Genetics Camera Studio, Windows OS 10 or higher
Saved Image Format
TIFF, JPEG
Image storage
Host computer dependent
Interface
Host computer dependent
Host computer dependent
Supports Mitsubishi thermal-printer P95D
Darkbox
Access
Front door, 180°opened
Power unit
Power supply for transilluminator and camera
Filter
FastGene®amber filter shield
Illuminator
Built-in Blue-Green light source
470-520 nm
View Area
26 x 21 cm
Unit
Material
Painted aluminum metal
Rated Voltage
100-240 V~, 50/60 Hz
Dimension (H x L x W)
52 x 33.5 x 32.5 cm
Weight
14 kg

Manual <GP-07LED>
Nomenclature
•Blue/Green LED XL-Transilluminator
•Darkbox
•Amber goggles
•FastGene ® amber filter shield attached with magnets (stored in the door)
•Camera holder
•USB-Stick with Software
•Cables:
a. Battery adapter for the camera
b. USB-Cable to connect the camera to the PC
c. Power cable 1: From a power socket to the darkbox
d. Power cable 2: From the darkbox to the Blue/Green LED XL-Transilluminator
•Camera:
a. Attached to the aluminum adapter (Please do not remove the aluminum adapter)
b. FastGene®amber filter attached by magnets to the lens
Aluminum adapter
Camera
Camera holder
Battery adapter
Darkbox
B/G LED
XL-Transilluminator

Manual <GP-07LED>

Manual <GP-07LED>
Part I
Installation

Installation <GP-07LED>
Installation guide –Setting up the FAS-Digi PRO
Place the unit on a level surface with minimal chance of dropping.
Assembling the camera holder
The camera holder prevents the camera from turning when rotating the zoom ring using the
aluminum adapter and is fixed by two screws to the darkbox and one tripod screw to the camera.
The position of the camera holder must be adjusted according to the camera:
1. Place the camera with its aluminum adapter in its final position, by adding it to the hole in the top
part of the darkbox.
2. Add the Battery adapter to the camera. Please note that only one direction is possible.
3. Close the battery compartment completely. Please put the cable in the cable channel. Please note:
the camera will not turn on if the battery compartment is not completely closed.
Cable channel

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
11
4. Align the camera, so that the image of the position is straight. The control buttons should be
facing toward the door of the darkbox.
5. Loose the tripod screw from the camera. Please be careful not to lose the spacer. The spacer is
added between the camera and the camera holder.
6. Fix the camera holder on the camera using the tripod screw in the following order:
Screw→Spacer→Camera holder→Spacer→Camera.
7. Align the camera holder holes with the 2 small holes in the darkbox
Tripod screw
holder
Spacer

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
12
8. Use the screws fixed on the box. You will need a 6mm head for the nut and a hexagon socket
screw (a.k.a. Allen) key.
The darkbox connected with the camera is now ready and can be placed on top of the
transilluminator. The next step will explain how to connect the cables.

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
13
Connecting the cables
The FAS-Digi PRO was designed to have only one power supply. The transilluminator and the
camera receive their electrical power from the darkbox. Please connect the cables using the
following order:
9. Connect the B/G LED XL-Transilluminator to the darkbox using the male to female power
plug:
10. Add the power lead to the power socket and to the darkbox.
11. Switch on the red switch on the back part of the darkbox.

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
14
The FAS-Digi PRO is now powered up. The transilluminator should switch on, as soon as the switch
in the front is used. The next step explains how the camera is connected to a PC.
Connecting the camera to a PC
The FAS-Digi PRO is powered up and can be used without a software. Here, we will explain how
to connect the FAS-Digi PRO gel documentation system to a Windows 10 PC and to use the
NIPPON Genetics Camera Studio software.
Preparation:
•Check that the autofocus and the image stabilization are turned off.
•Copy the FAS-Digi PRO folder to the desktop of a Microsoft Windows 10 PC.
•Connect the camera to the PC using the USB cable found in the camera box. The USB
port is on the left side of the camera.
•Set the camera to manual mode by turning the mode dial to M.
•Switch the camera on.
USB-Port
Focus & Image Stabilization

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
15
Starting the software
1. Start the NIPPON Genetics Camera Studio software:
Please note if a blue SmartScreen appears: select More info and chose the option run
anyway.
This blue SmartScreen will only appear when the NIPPON Genetics Camera Studio is
launched for the first time.

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
16
2. Log in
The NIPPON Genetics Camera Studio Software is password protected to avoid unauthorized
people to have access to the gel documentation system. The password to enter the software
is
NgE
3. The software will open.
You completed the installation of the FAS-Digi PRO. Please continue with the IQ/OQ/PQ protocol.
WELL DONE!

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
17

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
18

Installation <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
19
Part II
IQ – OQ - PQ

IQ, OQ, PQ <GP-07LED>
NIPPON Genetics EUROPE GmbH
Binsfelder Straße 77 | 52351 Düren | Germany
www.nipppongenetics.eu
Tel.: [phone]
Fax: [phone]
[e-mail]
[e-mail]
20
Installation, Operation and Performance Qualification
FAS-Digi PRO GP-07LED
Protocol Acceptance by Customer and List of Tests Performed
NIPPON Genetics EUROPE recommends signing the IQ, OQ, PQ document when all tests are
performed after installation. Operational Qualification should be run if the instrument is not
performing to specifications. This document is used as the final QC-protocol of every unit we
produce in Germany. Therefore, we use the lane FC (Factory Control) for our tests.
I have reviewed the IQ/OQ/PQ document and agree that it provides the appropriate procedures
for the Installation Qualification/Operational Qualification and Performance Qualification of the
FAS-Digi PRO device and software.
Reviewer Name
Signature
Date
Date
Signature
Customer Name