Nippon Genetics Fast Gene NG020A User manual

NG020A
Manual
NIPPON Genecs EUROPE GmbH Mariaweilerstraße 28-30, 52349 Düren, Germany,
Tel: [phone] Fax: [phone], E-Mail: info@nippongenecs.de, www.nippongenecs.eu
Mini Dry Bath Advance

NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance

NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance 3
Content
CONTENT
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1. GENERAL INFORMATION...................................................................
1.1 Introducon...............................................................................
1.2 First QC Check..........................................................................
2. SAFETY......................................................................................................
2.1 Safety Measures........................................................................
2.2 Safety Informaon....................................................................
2.2.1 Environmental Condions.......................................
2.2.2 Avoid Electrical Shock..............................................
2.2.3 Avoid Damage to the Instrument..........................
3. MANUAL...................................................................................................
3.1 Specicaons.............................................................................
3.2 Device Components.................................................................
3.3 Installaon Guide......................................................................
3.4 Operaon Guide.......................................................................
3.4.1 Key Funcons.............................................................
3.4.2 Program Seng.........................................................
3.4.3 Program Execuon....................................................
3.5 Temperature Calibraon..........................................................
3.5.1 Calibraon Setup.......................................................
3.5.2 Calibraon Procedure...............................................
3.6 Automac Heang....................................................................
3.7 System Maintenance................................................................
3.8 Troubleshoong.........................................................................
4. ORDERING INFORMATION................................................................
5. WARRANTY..............................................................................................
5. CONTACT INFORMATION...................................................................

NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
1. GENERAL INFORMATION
1.1 Introducon
Thank you for purchasing the FastGene® Mini Dry Bath Advance, a
micro-processor controlled block heater. Applications include sample
and reaction tempering, sample heating, denaturation of electrophoresis
samples, serum coagulation and many more. This manual includes the
operation guide. In order to use the instrument properly, please read this
manual carefully before using the FastGene®Mini Dry Bath Advance. This
instrument is suitable for research use only.
1.2 First QC check
Please check the instrument and the packing list when you rst open the
packing case. If you nd there is something wrong with the instrument or
the packaging content, please contact Nippon Genecs Europe or your
local distributor.
Packing List:
FastGene® Mini Dry Bath Advance - Packing list Quanty
FastGene® Mini Dry Bath Advance 1
Transparent Cover 1
Power Cord 1
Power Adapter 1
Manual 1
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NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
2. SAFETY
2.1 Safety Measures
The FastGene®Mini Dry Bath Advance has been tested and found to
comply with safety limits for the CE regulaon. These limits are designed
to provide reasonable protecon against harmful interference when
the equipment is operated in a commercial environment. Changes
or modicaons not expressly approved by the party responsible for
compliance could void the user’s authority to operate the equipment. It is
strongly recommended for the user to read the following points carefully
before operang this equipment:
• Read and carefully follow the manual instrucons.
• Do not alter the equipment, as it could result in personal and/
or laboratory hazards, as well as invalidate equipment warranty.
• Use the device indoors only.
• The device should be placed in a room with low humidity, lile
dust and in secure distance from water or heat sources. Avoid
exposure to direct sunlight or strong light sources.
• Use a properly grounded electrical outlet with correct voltage
and current capacity.
• Disconnect from power supply before maintenance and servicing.
Refer servicing to qualied personnel.
• Do not use this instrument without having the safety cover
correctly in posion.
• Do not use the unit if there is any sign of damage to the external
tank or cover. Replace damaged parts.
• Do not use the unit in the presence of ammable or combusble
materials, as it may result in re or explosion. This device contains
components which may ignite such materials.
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NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
• Ensure that the system is connected to electrical service according
to local and naonal electrical codes. Failure to properly connect
the device may lead to re or shock hazard.
• Use appropriate materials to avoid possible hazards of explosion,
implosion or release of toxic or ammable gases arising from
overheated materials.
• Wear appropriate protecon to avoid burning your hand.
2.2 Safety Informaon
Use high level of precauon when using any electrical device. Before
connecng the electrical supply, check to see if the supply voltage is
within the range stated at the rang label. Place the unit in a safe and dry
locaon. It must not touch anything nearby. Follow the safety precauons
for chemicals and dangerous materials. If needed, please contact a
qualied service representave or info@nippongenecs.eu.
2.2.1 Environmental Condions
Ensure the instrument is installed and operated strictly under the
following condions:
• Indoor use only
• ≤95% RH
• 75 kPa – 106 kPa
• Altude must not exceed 2,000 meters
• Ambient to 40°C operang temperature
• Mains supply voltage uctuaons up to ±10% of the normal
voltage
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NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
2.2.2 Avoid Electrical Shock
Follow the guidelines below to ensure a safe operaon of the unit. The
FastGene®Mini Dry Bath Advance has been designed with shielded wires,
thus minimizing any potenal shock hazard to the user. Nippon Genecs
EUROPE disadvises the use of unshielded wires.
To avoid electrical Shock:
• If a soluon is accidentally spilled into the instrument, it must be
dried out for at least 2 hours, and restored to a normal condion.
• Never connect or disconnect wire leads from the power jacks
when the power is on.
• Wait at least 5 seconds aer stopping a run before handling
output leads or the connected device.
• Always make sure that hands, working area and instruments are
clean and dry before making any connecons or operang the
device.
• Only connect the power cord to a properly grounded AC outlet.
2.2.3 Avoid Damage to the Instrument
To avoid damage to the instrument:
• Do not aempt to operate the device if it is damaged.
• Protect the unit from physical damage, corrosive agents and
extreme temperatures.
• For proper venlaon, keep at least 10 cm of space behind the
instrument and at least 5 cm of space on each side.
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NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
3. MANUAL
3.1 Specicaons
The FastGene® Mini Dry Bath Advance provides excellent temperature
control for a wide variety of applications and delivers accurate and reliable
experimental results.
Specicaons NG020A
Temperature Control Range room temp. (+5 °C) - ~100 °C
Heang Time (20 °C to 100 °C) ≤ 18 min
Temperature Accuracy Discrepancy ± 0.3 °C
Temperature Control Discrepancy @ 40 °C ± 0.3 °C
Temperature Control Discrepancy @ 100 °C ± 0.3 °C
Temperature Accuracy Display 0.1 °C
Time Seng 0-99 h, 0-99 min, 0-99 sec
Max. temperature 100 °C
Max. Power 50 W
Cooling Time Natural Cooling
Weight ≤ 1 kg
Dimensions (H x D x W) 13.6 x 16 x 11 cm
With Transparent Plasc Cover Yes
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FastGene® Mini Dry Bath Advance
3.2 Device Components
Please refer to the following page for the locaon of controls and features:
Front:
Back:
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NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance
3.3 Installaon Guide
1. Place the FastGene® Mini Dry Bath Advance on a stable
workbench.
2. Ensure that the main power switch at the back of the instrument
is turned off. Insert the power cord into the power port on the
back of the instrument as shown in the figure below. Connect
the other end of the external power cord to the power grid.
3. Switch on the device by pressing the main switch to (I).
4. After the device is turned on, it will automatically heat up to the
set temperature for 5 min.
5. Put the tubes into the the wells of the heating block and cover it
with the transparent heating block cover.
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FastGene® Mini Dry Bath Advance
3.4 Operaon Guide
3.4.1 Key Funcons
Start/Stop...................................... Operaon and control of the device
Prog................................................. Program buon, set the device
(up and down)................... Set program, temperature and me
(le).......................................... Move le to choose parameter values
(right)........................................ Move right to choose parameter values
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FastGene® Mini Dry Bath Advance
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3.4.2 Program Seng
Nine different programs can be set for the FastGene® Mini Dry Bath
Advance (P1 - P9), including 3 steps for each program (e.g. P2A, P2B
and P2C for program P2). Aer switching on the device, the monitor will
display the following values:
P1A 23.5 NOTOK
Press the up or down buons to navigate through the dierent programs
(P1 - P9) and the le or right buons to navigate and view the dierent
program steps (A, B, C). All programs can be set manually. To change a
program press the program buon (Prog). The program number starts
ashing:
P1A 028.0 12m
Press the up or down buon to choose the program you would like to
change (e.g. naviagte to P3).
P3A 037.0 20m
Press the right buon once to switch to the program steps (program
step starts ashing) and use the up or down buons to choose a specic
program step (e.g. step B):
P3B 060.0 10m
To set the temperature of the specic program step, press the right buon
unl the gures of the temperature start ashing and set the individual
digits by pressing the up or down buon (e.g set to 85 °C).

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FastGene® Mini Dry Bath Advance 13
P3B 080.0 10m
P3B 085.0 10m
To set the me of a specic program step, press the right buon unl the
gures of the me start ashing and set it by pressing the up or down
buon (e.g. set to 30 min).
P3B 085.0 30m
To set the me unit of a specic program step, press the right buon
unl the me unit starts ashing and set it by pressing the up or down
buon. The me unit can be set to seconds (s), minutes (m) or hours (h).
For program steps B and C the me unit can also be set to "OFF", which
leads to skipping the step.
P3C 085.0 30 OFF
In this example, the following step parameters have been set for program 3
(P3):
P3A 037.0 20m
P3B 085.0 30m
P3C 085.0 30 OFF

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FastGene® Mini Dry Bath Advance
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3.4.3 Program Execuon
Aer seng the parameters of the individual program steps, the program
(P3 shown as example) is started by pressing the Start/Stop buon. The
current temperature is displayed. Unl the desired temperature of step A
is reached, the monitor will display "WAIT".
P3A 23.5 WAIT
Aer the device has reached the desired temperature, the monitor will
display "OK" and the me countdown will be started.
P3A 37.0 20m OK
When the me of the rst program step (e.g. P3A) is completed, the
next steps of the program will be executed automacally. The device will
display "WAIT" unl the desired temperature of step P3B is reached:
P3B 38.4 WAIT
P3B 85.0 30m OK
As the thrid step of the program (P3C) has been set to "OFF", this step is
skipped and the program P3 has ended. The compleon of the program
is indicated by an alarm and displayed with "FINISHED" and the current
heang block temperature:
FINISHED 85.0
Press the Start/Stop buon to return to the program selecon page.

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FastGene® Mini Dry Bath Advance 15
Note: To pause the program, press the Start/Stop buon. The paused
program is displayed by a "P":
P3B 85.0 24m P
To abort the program, press the Start/Stop buon for 2 seconds.
Note: If the FastGene® Mini Dry Bath Advance needs to be used at a very
accurate temperature (± 0.3 °C), we recommend to preheat the thermo
block for ~20 min.

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FastGene® Mini Dry Bath Advance
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3.5 Temperature Calibraon
The temperature calibraon is carried out by the manufacturer but can
be recalibrated by following the steps specied below.
Note: Please do not aempt to recalibrate the temperature unless
necessary. If the instrument needs recalibraon, please place it at room
temperature below 35 °C.
3.5.1 Calibraon Setup
For calibraon, inject olen/paran oil into one of the cone-chaped
wells and place a thermometer into this well. Make sure the precision
of the thermometer is within 0.1 °C. The p of the thermometer should
be completely immerged in the oil. Use heat isolaon material on the
thermoblock to separate it from the surrounding (see gure below).

NIPPON Genecs EUROPE
FastGene® Mini Dry Bath Advance 17
3.5.2 Calibraon Procedure
Press the le and right buon simultanously to start the calibraon
procedure. The thermo block automacally heats up to 40 °C. During
the heang procedure the "*" symbol is ashing. When the temperature
reaches 40 °C the ashing of "*" stops. Five minutes later, the alarm
begins to sound regularly.
T1 23.5 ADJ *
T1 40.0 ADJ *
Read the temperature on the thermometer aer 20 min of incubaon. If
the thermometer reads e.g. 39.8 °C, use the up or down buons to set it
to 39.8 °C. Press the Start/Stop buon to corm the correcon.
The thermoblock automacally heats up to 70 °C. During the heang
procedure the "*" symbol is ashing. When the temperature reaches
70 °C the ashing of "*" stops. Five minutes later, the alarm begins to
sound regularly.
T2 41.7 ADJ *
T2 70.0 ADJ *
Read the temperature on the thermometer aer 20 min of incubaon. If
the thermometer reads e.g. 69.8 °C, use the up or down buons to set it
to 69.8 °C. Press the Start/Stop buon to corm the correcon.
Repeat this procedure analogously for 100 °C.
Return to the program selecon page and if needed correct the
temperature sengs.

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FastGene® Mini Dry Bath Advance
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Note: Aer the temperature is calibrated, the measured temperature of
the module is consistent with the displayed temperature.
Note: Press the program buon (Prog) to cancel the current temperature
calibraon. The system will default to the last calibraon sengs.
In order to ensure the accuracy, the instrument must keep a constant
temperature for 20 min aer reaching any calibraon point.
3.6 Automac Heang
Press the program buon (Prog) for 2 sec to enter the automac heang
sengs. Press the Start/Stop buon and use the up or down buon to
choose "APF: ENABLE" or "APF: DISABLE".
APF: ENABLE
APF: DISABLE
With "APF: DISABLE", the device will not automacally heat up aer
turning it on. The device will heat up aer pressing the Start/Stop buon.
Press the program buon (Prog) to save the current sengs and return
to the program selecon page.
Note: The default seng is "APF: ENABLE". With this seng, the system
will automacally heat up aer turning it on.

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FastGene® Mini Dry Bath Advance 19
3.7 System Maintenance
The wells of the thermoblock should be cleaned regularly with a cloth
soaked in alkohol, to remove dust and dirt and assure good heat transfer
between the block and the tubes.
Turn the power o when cleaning the instrument. Do not pour cleaning
liquid into the wells and do not use corrosive cleaning liquids to clean the
instrument.
3.8 Troubleshoong
Problem Cause Soluon
No signal on the monitor
No power
Switch broken
Control Unit broken
Check power connecon
Exchange switch
Contact seller
Monitor displays "OPEN"
with alarm sound
Open circuit of
temperature sensor Contact seller
Monitor displays
"SHORT" with alarm
sound
Short circuit of
temeprature sensor Contact seller
Block not heang Broken heater Contact seller
Buon not working Broken buon Contact seller

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FastGene® Mini Dry Bath Advance
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4. ORDERING INFORMATION
The FastGene® Mini Dry Bath Advance can be equipped with dierent
tube size thermoblocks:
Product Catalogue Number
FastGene®Mini Dry Bath Advance NG020A
Block for 32x 0.2 ml tubes NG025A
Block for 24x 0.5 ml tubes NG029A
Block for 15x 1.5 ml tubes NG026A
Block for 15x 2.0 ml tubes NG030A
Block for 12x 5 ml tubes NG024A
Block for 6x 15 ml tubes NG027A
Block for 2x 50 ml tubes NG028A
Note: When using the thermoblocks NG027A for 6x 15 ml tubes and
NG028A for 2x 50 ml tubes, the temperature needs to be set to a higher
value.