Infors HT Multitron Standard User manual

Multitron Standard
Operating Manual
2021-03-17
We bring life to your laboratory.

Highest capacity for
microorganisms
Multitron Standard
Incubation Shaker
Doc-Nr. 79325 - EN
V.05.00 - Original
This operating manual can also be found
online at:
https://www.infors-ht.com/en/multitron-
standard
Infors AG
Headoffice, Switzerland
Rittergasse 27
CH-4103 Bottmingen
T [phone]
[e-mail]
[e-mail]

Multitron Standard - Operating Manual
Table of Contents
17 March 2021 Page 1of 114
1General Information...............................................................5
1.1 About this Manual ..........................................................5
1.2 Explanation of Special Notices.........................................6
1.2.1 Warning Notices ..............................................6
1.2.2 Other Notices...................................................6
1.3 Device Identification (Standard Identification Plate) .........7
1.4 Declaration of Conformity...............................................7
1.5 Customer Service and Services ........................................7
2Safety and Responsibility ......................................................8
2.1 Intended Use, Incorrect Use and Misuse ..........................8
2.2 Cultivation Vessels to Be Used.........................................9
2.3 Qualified Personnel .......................................................11
2.3.1 Provider .........................................................11
2.3.2 User...............................................................11
2.3.3 Operator........................................................12
2.4 Unauthorised Persons....................................................13
2.5 Responsibility of the Provider.........................................13
2.6 General Hazards............................................................13
2.6.1 Electrical Current ...........................................14
2.6.2 Danger due to Moving Parts ..........................14
2.6.3 Unauthorised Spare Parts and Accessories .....14
2.7 Particular Hazards..........................................................15
2.7.1 Danger due to Hot Surfaces...........................15
2.7.2 Dangerous Gases...........................................15
2.7.3 Flammable or Explosive Substances................15
2.7.4 Corrosive or Toxic Substances........................16
2.7.5 Pathogenic Organisms ...................................16
2.8 Safety Features..............................................................16
2.9 Warning Symbols on the Device....................................17
2.10 Declaration of Decontamination....................................18
3Setup and Function ..............................................................19
3.1 Setup of the Basic Unit..................................................19
3.2 Overview of Versions.....................................................21
3.3 Functions ......................................................................23
3.3.1 Standard Function - Shaking..........................23
3.3.2 Standard Function – Temperature Control
(Heating and Ventilation) ...............................25
3.3.3 Optional Cooling Function .............................26
3.4 Interior Lighting.............................................................27

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3.5 Connections and Interfaces ...........................................27
3.5.1 Mains Connection .........................................27
3.5.2 Serial Interface...............................................28
3.6 Openings ......................................................................28
3.6.1 Discharge Outlet............................................28
3.6.2 Air Vents........................................................29
3.7 Substructure..................................................................29
3.8 Operating and Indicating Elements................................30
3.8.1 Power Switch.................................................30
3.8.2 Operating Panel.............................................30
3.9 Markings on the Device................................................. 31
3.9.1 Identification Plate .........................................31
3.9.2 Identification of the Throw ............................31
4Accessories ............................................................................32
4.1 Trays .............................................................................32
4.1.1 Universal Table Tray .......................................32
4.1.2 Pre-Fitted Trays ..............................................33
4.1.3 Tray with Sticky Stuff .....................................38
4.2 Clamps and Other Holders ............................................41
4.2.1 Clamps .......................................................... 41
4.2.2 Test Tube Holders ..........................................42
4.3 Box for Microtitre Plates ................................................43
4.4 eve®.............................................................................. 46
5Installation ............................................................................ 47
5.1 Operating Conditions at the Installation Location..........47
5.2 Requirements for the Mains Connection .......................48
5.3 Minimum Distances to the Device .................................49
6Operation ..............................................................................51
6.1 Switching on the Device................................................51
6.2 Loading the Device........................................................51
6.2.1 Opening the Door..........................................52
6.2.2 Removing the Tray.........................................53
6.2.3 Inserting the Tray...........................................54
6.2.4 Handling Cultivation Flasks without
Removing the Tray.........................................55
6.2.5 Fitting the Holders .........................................56
6.2.6 Tips & Tricks for Loading the Tray ..................58
6.3 Overview of Display and Operating Elements ................60
6.3.1 Operating Elements .......................................61

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6.3.2 Display Elements............................................62
6.3.3 View Boxes ....................................................63
6.4 Adjusting, Activating and Deactivating Parameters........65
6.4.1 Overview of Parameters .................................65
6.4.2 Setting the Parameter Setpoint ......................66
6.4.3 Activating and Deactivating Parameters .........67
6.5 Timer Function .............................................................. 69
6.5.1 Overview .......................................................69
6.5.2 Programming a One-Time Change.................72
6.5.3 Programming a Cyclical Change ....................74
6.5.4 Displaying the Remaining Time ......................77
6.5.5 Aborting the Timer Function ..........................78
6.6 Operating the Operating Hours Counter .......................79
6.7 Switching on the Interior Lighting .................................80
6.8 Switching off the Device ...............................................80
6.9 Behaviour in Case of Interrupted Power Supply.............81
7Rectifying Faults ...................................................................82
7.1 Messages in the View Boxes..........................................82
7.1.1 Alarm Messages and their Meanings .............82
7.1.2 Error Messages and their Meanings ...............85
7.2 Fault Tables...................................................................86
7.3 Rectifying Faults ............................................................90
7.3.1 Replacing Fuses..............................................90
7.3.2 Replacing the Lighting Element for the
Interior Lighting .............................................90
7.4 Returning for Repair......................................................91
8Maintenance and Cleaning..................................................92
8.1 Maintenance.................................................................92
8.2 Cleaning and Disinfection .............................................93
8.2.1 Cleaning ........................................................93
8.2.2 Disinfection....................................................95
9Transport and Storage .........................................................96
9.1 Transport ......................................................................96
9.2 Storage ......................................................................... 96
10 Disassembly and Disposal....................................................97
10.1 Disassembly...................................................................97
10.2 Disposal ........................................................................98

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11 Technical Data and Specifications.......................................99
11.1 Dimension Drawings .....................................................99
11.2 Specifications of the Basic Device ................................101
11.2.1 Weight and Dimensions...............................101
11.2.2 Electrical Connection and Performance
Values..........................................................102
11.2.3 Interior Lighting ...........................................103
11.2.4 Materials......................................................103
11.2.5 Emissions.....................................................103
11.2.6 Operating Conditions ..................................104
11.2.7 Protection Type............................................104
11.2.8 Operating and Auxiliary Materials ................105
11.3 Specifications of Parameters........................................105
11.3.1 Rotation Speed Parameter (Shaker Drive) .....105
11.3.2 Temperature Parameter ...............................106
11.4 Parameter Limits..........................................................106
11.4.1 Maximum Permissible Rotation Speed
Setpoints .....................................................107
11.4.2 Information on Temperatures that can
Actually be Achieved ...................................108
12 EC-Declaration of Conformity ...........................................109
13 Index....................................................................................110

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General Information
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1General Information
1.1 About this Manual
This manual enables the safe and efficient handling of the device.
All the information and instructions in this operating manual comply
with the current standards, legal regulations, the latest technological
and scientific developments and the knowledge gained from the
manufacturer’s many years of experience in this field.
This operating manual is a component part of the device. It
must be kept near to the device and must be accessible to the
operators at all times.
The users must read the operating manual thoroughly and fully un-
derstand its contents before beginning any work.
Adhering to all the safety and operating instructions in this manual is
essential to ensure that work is carried out safely.
The scope of delivery may differ from the explanations, descriptions
and figures in this operating manual due to special designs, addi-
tional options specified on ordering and the latest technical/mechani-
cal modifications.
This manual contains illustrations to aid general understanding. These
may differ from the actual device as supplied.

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1.2 Explanation of Special Notices
1.2.1 Warning Notices
Warning notices in this manual are indicated by a coloured bar and
begin with a signal word that signifies the degree of the hazard.
DANGER
The signal word “DANGER” indicates a dangerous situation that
will lead to severe or even fatal injuries if not avoided.
WARNING
The signal word “WARNING” indicates a potentially dangerous sit-
uation that may result in severe or even fatal injuries if not
avoided.
CAUTION
The signal word “CAUTION” indicates a potentially dangerous sit-
uation that may result in minor injuries if not avoided.
1.2.2 Other Notices
ATTENTION
The word “ATTENTION” on a blue bar indicates a situation that
may result in significant damage to property if not avoided.
INFORMATION
Texts located below a grey bar bearing the notice “INFOR-
MATION” provide useful tips and recommendations for ensuring
efficient, fault-free operation of the device.

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1.3 Device Identification (Standard Identification Plate)
The identification plate is designed to allow clear identification of the
device. It contains the following information:
Manufacturer name
Designation = Category of device
Type = Device type (name)
S/N = Serial number
Year = Year of manufacture
Mains = Nominal voltage and frequency
Current = Current consumption
Manufacturer address
CE marking
1.4 Declaration of Conformity
The device is in compliance with the essential requirements of the
following Directives:
Machinery Directive 2006/42/EC
EMC Directive 2014/30/EU
The Declaration of Conformity according to EC Machinery Directive
2006/42/EC, annex II 1 A is attached to the operating manual (see
chapter 12 "EC-Declaration of Conformity", page 109).
1.5 Customer Service and Services
Our Customer Service is at your disposal for technical advice and spe-
cialist enquiries. For contact information, see page 2.
Due to their familiarity with the potential applications of the device,
the Customer Service team is able to provide information on whether
the device can be used for a specific application or modified to han-
dle the planned process.
Furthermore, our colleagues are always interested in new information
and experiences resulting from user’s applications for the device that
may be valuable for the continued development of our products.

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2Safety and Responsibility
This section describes general considerations relating to user safety
that must be taken into account when working with the device.
In the remaining sections, warning notices are used only to highlight
particular hazards directly arising from the actions being described in
the section in question.
It is essential to read the operating manual carefully – espe-
cially this section and the warning notices in the text – and to
follow the instructions therein.
This section also refers to areas that are the responsibility of the pro-
vider due to certain risks arising from particular applications for which
the device is used deliberately and with full awareness of the associ-
ated risks.
2.1 Intended Use, Incorrect Use and Misuse
Depending on its features, the device is designed to be used
as an incubation shaker for cultivating
microorganisms or cell
cultures under the following conditions:
Cultivation of non-pathogenic microorganisms or cell
cultures of risk category 1 in a biotechnology labora-
tory of biological protection level 1.
Cultivation of pathogenic microorganisms or cell cul-
tures of risk category 2 in a biotechnology laboratory
of biological protection level 2.
When using the device in protection level 2, users are respon-
sible for taking appropriate protective measures to ensure
that organisms cannot escape uncontrollably due to flask
breakage, unintentional detaching of the sterile seal or simi-
lar.
WARNING
The device is designed and constructed exclusively for the intended
use described above.
Each instance of non-conventional use is considered as misuse and
may lead to dangerous situations.

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Intended use also includes following all the instructions in this man-
ual, especially those relating to:
The installation site
Use of suitable cultivation vessels
User qualifications
Permissible parameter setpoints
Correct operation and maintenance
Any failure to observe the requirements specified in this manual shall
be deemed incorrect use, in particular, use of inappropriate cultiva-
tion vessels and/or unsuitable holders at speeds that are too high.
Any use of the device outside the scope of the intended use as de-
scribed above shall be deemed misuse. This also applies to applica-
tions for which the device is not designed, especially the following:
The device is not protected against explosions. Use and manu-
facture of explosive gases as well as operating the device in the
Ex area are therefore not permitted.
The device is not designed to sufficiently protect its users if path-
ogenic organisms escape uncontrollably. Cultivation of patho-
genic organisms of risk categories 3 and 4 is therefore not per-
mitted.
To use the device for special applications not covered by conven-
tional, intended use, the device must be modified and certified ac-
cordingly by the manufacturer.
Any use of the device outside of a biotechnology laboratory, i.e. in
any environment in which the conditions required for the safety of
users cannot be met or cannot be met to their full extent, shall also
be deemed misuse.
2.2 Cultivation Vessels to Be Used
Significant forces are applied to cultivation vessels, in particular in
case of large vessels and high speeds. Hence, the cultivation vessels
used are particularly significant in relation to user safety.
ATTENTION
Use of unsuitable or defective cultivation vessels can lead to glass
breakage and therefore damage to property.

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Approved cultivation vessels
The device has been designed for use with the following vessels using
the holders designed specifically for them:
Erlenmeyer flasks up to 5,000 mL made of borosilicate glass
(e.g. Schott Duran®) or high-grade plastic, such as polycarbonate
(z. B. Corning®) etc.
Fernbach flasks up to 3,000 mL made of borosilicate glass (e.g.
Schott Duran®) or high-grade plastic, such as polycarbonate
(z. B. Corning®) etc.
Other vessels with the holders designed for them:
Test tubes
Centrifuge tubes
Microtitre plates
Deep well plates
To avoid the vessels coming out of the clamps at very high speeds,
they might have to be secured using cable ties underneath the
springs or some other suitable measure.
Cultivating organisms of risk category 2
When cultivating pathogenic organisms of risk category 2, special
measures must be taken to stop the organisms from escaping. The
user is responsible for this.
When using the device under protection category 2, stainless steel
clamps of the correct size must be used to affix the flasks. Due to lim-
ited resistance to disinfectants as well as the risk of unintentional de-
taching of flasks, Sticky Stuff adhesive matting is not suitable for this
purpose.
We further recommend using disposable plastic flasks with screw
tops and filter membranes. We recommend using sticky tape to se-
cure the lid against loosening unintentionally. Using glass flasks with
cotton wool or paper plugs is not sufficiently safe.
Trays with Sticky Stuff
INFORMATION
For trays with Sticky Stuff, special provisions apply in relation to
maximum permitted speeds. These must be observed to prevent
cultivation vessels from detaching.
For detailed information see chapter 4.1.3 "Tray with Sticky
Stuff", page 38.

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2.3 Qualified Personnel
Due to the complexity of the device and the potential risks arising
from its operation, the device may only be used by qualified, special-
ist personnel.
2.3.1 Provider
The term “provider” applies to all persons who are responsible for
making the device and the necessary infrastructure available. These
persons may also be included in the group of people known as “us-
ers”, though this is not always the case.
Irrespective of whether a provider is a member of the company’s
board of management or a supervisor, they bear a special level of
responsibility with regard to the processes and the qualification and
safety of the users.
2.3.2 User
General
The term “user” applies to all persons who come into contact with
the device in any way and perform work on or with it. This primarily
applies to the following activities, which can be performed by the
manufacturer’s own specialists or a variety of other persons (it is not
always possible to distinguish clearly between the different types of
person):
Assembly, installation and commissioning
Definition and preparation of the process
Operation
Troubleshooting and remedying of faults
Maintenance and cleaning (autoclaving, if necessary)
Service work and repairs
Disassembly, disposal and recycling
Qualified personnel
On account of their specific education, training and – in many cases –
experience, the qualified personnel required for this work are able to
recognise risks and respond accordingly to potential hazards.
The qualified personnel (either internal or external) who cannot be
categorised under the separate “operators” group are made up of
the following groups of persons:

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Electricians (electrical engineers)
Decontamination specialists
Repair specialists
Specialists in disassembly and (environmentally friendly) disposal
Recycling specialists
2.3.3 Operator
The “operators” are a specific sub-group of users distinguished by
the fact that they work with the device. They are the true target audi-
ence for this operating manual.
Qualified technicians
Only technicians who have been trained for working in a biotechnol-
ogy laboratory can be considered for the role of operator. These in-
clude:
Process technicians in the fields of biotechnology and chemistry
Biotechnologists (biotechnicians)
Chemists with a specialisation in biochemistry; chemists in the
field of organic chemistry or biochemistry
Life scientists (biologists) with special education in cytology, bac-
teriology, molecular biology, genetics, etc.
Lab assistants (lab technicians) from various fields
In order to be classed as a “sufficiently qualified technician” for the
operation of the device, the persons in question must have received
thorough training and have read and understood the operating man-
ual.
The operator must be informed in a training session provided by the
provider of the tasks delegated to the operator and the potential risks
of improper conduct. Tasks that go beyond the scope of operation
under normal conditions may only be performed by the operator if
this is specified in the manual and the provider has explicitly en-
trusted said tasks to the operator.
Technicians in training
Persons in this group who are undergoing training or apprenticeships
are only permitted to use the device under supervision and in accord-
ance with the instructions of a trained and qualified technician.

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2.4 Unauthorised Persons
The term “unauthorised persons” applies to all persons who can ac-
cess the work area but are not qualified to use the device in accord-
ance with the aforementioned requirements.
Unauthorised persons are not permitted to operate the device or use
it in any other way.
2.5 Responsibility of the Provider
The device is used for industrial and scientific purposes. As such, the
provider of the device is individually liable with regard to the legal
requirements relating to occupational health and safety in a biotech-
nology laboratory. In particular:
The provider is responsible for ensuring that the work and envi-
ronmental regulations applicable in a biotechnology laboratory
are observed.
The provider must ensure that the device remains in safe and
proper working condition throughout its entire term of use.
The provider must ensure that all safety equipment is fully func-
tional and is not disabled.
The provider must ensure that the device is only worked on by
qualified users, and that said users receive sufficient training.
The provider must ensure that the protective equipment re-
quired for working with the device is provided and worn.
The provider must ensure that this operating manual remains in
the immediate vicinity of the device throughout its entire term
of use.
2.6 General Hazards
This section covers general hazards and residual risks that are always
present when using the device in accordance with normal, intended
use.
The following notices are general in nature. As such, with a few ex-
ceptions they are not repeated in the remaining sections.

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2.6.1 Electrical Current
The device is operated electronically. There is an immediate risk of fa-
tal injury if contact is made with live parts.
The following points must be observed in order to avoid the risk of
fatal injury:
In case of damage to insulation, disconnect the device from the
mains immediately and arrange for it to be repaired.
Disconnect the device from the mains before commencing any
work on the electrical system.
Always use qualified electricians for any work on the electrical
system.
Disconnect the device from the mains before beginning any
maintenance, cleaning or repair work.
Do not bypass any fuses or take them out of operation.
When replacing fuses, ensure they have the correct number of
Amperes.
If the power cable is defective, replace it with a power cable of
the same type.
Keep moisture away from live parts. It may lead to a short cir-
cuit.
Never remove covers from the casing.
2.6.2 Danger due to Moving Parts
There is a risk of injury from moving parts.
Body parts can be pinched
or scratched when one is not careful.
However, there is no danger that clothing or body parts can be
pulled into the device due orbital movement of the table.
The danger that fingers are pinched has been minimised by means of
a sufficient distance between the tray and the casing and a stop
mechanism that stops the shaker drive when the door is opened.
Nonetheless, the cultivation flasks must only be moved when the ta-
ble has come to a complete standstill.
2.6.3 Unauthorised Spare Parts and Accessories
Incorrect or imitated spare parts and accessories as well as spare parts
or accessories that have not been authorised by the manufacturer
represent a significant safety risk. As such, we recommend procuring
all spare parts and accessories from an authorised dealer or directly
from the manufacturer. For the contact details of the manufacturer’s
representatives, see page 2.

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2.7 Particular Hazards
This section covers particular hazards and residual risks that may arise
when using the device for special applications in accordance with
normal, intended use.
Since the use of the device for such applications is deliberate, it is the
responsibility of the operators and the provider to ensure that all per-
sonnel are protected from potential damage to health. The provider
is responsible for ensuring that the appropriate protective equipment
for such applications is provided, and that the necessary infrastruc-
ture is in place.
2.7.1 Danger due to Hot Surfaces
For applications that are performed with temperatures over 55 °C,
there is a danger of burns on hot surfaces in the interior or on the
cultivation vessels.
For applications with temperatures over 55 °C wear heat-re-
sistant protective gloves.
2.7.2 Dangerous Gases
The use or production of dangerous gases i.e. toxic or asphyxiant
gases entails a significant health risk, especially in enclosed spaces.
In order to prevent high emissions of dangerous gases, the following
measures must be taken:
The gas connections on the device must be checked before any
cultivation processes using dangerous gases are initiated.
The gaskets on the device must be checked at regular intervals
and replaced if necessary.
2.7.3 Flammable or Explosive Substances
The use or production of flammable or explosive substances is not
covered under “intended use” of the device, as the device is not ex-
plosion-proof.
If the provider intends to use the device for such purposes, he must
check its suitability for the planned application with the responsible
local authorities.

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2.7.4 Corrosive or Toxic Substances
The use or production of corrosive or toxic substances entails a signif-
icant health risk. As such, special measures must be taken to protect
the users for such applications.
Since the device is used deliberately for such applications, it is the
responsibility of the users to ensure that they have sufficient protec-
tion.
2.7.5 Pathogenic Organisms
The devi
ce is not approved for cultivation of pathogenic organisms of
risk categories 3 and 4. In the context of intended use, it is nonethe-
less possible for pathogenic organisms and viruses to be cultivated.
Contact with pathogenic organisms bears a significant health risk.
Hence, users are responsible for ensuring adequate protection.
2.8 Safety Features
The device comes with the following safety features:
Fuses
The device is protected from impermissibly high power input by two
fuses. The fuses are located right next to the mains connection on
the left-hand side of the casing. For descriptions of which fuses to
use for which device type, see chapter 11.2.2 "Electrical Connection
and Performance Values", page 102.
Overheating shut-down
The device is equipped with two forms of protection against over-
heating:
A capillary thermostat with a control knob (can be adjusted by
the owner; factory setting: 70°C)
A safety fuse (84°C)

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Door monitoring
The position of the door is monitored electronically. If the door is
opened, all dangerous movements (shaker drive and fan) are stopped
immediately. As soon as the door is fully closed again, the shaker
drive and fan restart automatically.
2.9 Warning Symbols on the Device
The following warning symbols (stickers) are attached to the device:
Position
On the left-hand side of the device casing.
Meaning
The device covers may only be opened by qualified electricians. Turn
off the device and pull out the power plug before commencing any
work on the electrical system.
Position
On the left-hand side of the top cooling system, or on the front of
the 31 mm base when using the base cooling system.
Meaning
If cooling systems with a cooling circuit (top and base cooling sys-
tems) are not filled with cooling liquid prior to commissioning, the
device may run dry, thus damaging the pump.
The cooling liquid is filled up by the manufacturer’s qualified person-
nel during initial commissioning.
WARNING
Illegible or missing warning symbols on the device will lead to the
user being exposed to risks that the warning symbols in question
were designed to make him or her aware of.
It is the provider’s responsibility to ensure that all the stickers with
warning symbols on the device are always intact.

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2.10 Declaration of Decontamination
When returning the device for repair, disassembly or disposal, it is
required for the safety of all parties involved and because of legal
provisions that a lawful declaration of decontamination is present.
The following must be observed if this is the case:
The device, the component part or accessory must be entirely
decontaminated before sending to the manufacturer
The provider is therefore required to completely and truthfully
fill out a declaration of decontamination, and have it signed by
the person responsible.
The declaration of decontamination must be affixed on
the outer packaging in which the device is sent back.
These forms can be obtained from the licensed dealer or the
manufacturer. See address on page 2.
Important notice
If the return shipment is not accompanied by a signed and complete
declaration of decontamination or it is not affixed to the outer pack-
aging, the shipment will be returned unopened to the sender at their
expense (see also T&C).