Agilent Technologies 1290 infinity User manual

Agilent Technologies
Agilent 1290 Infinity
Valve Drive and Valve Heads
User Manual

Agilent 1290 Infinity Valve Drive and Valve Heads
Notices
© Agilent Technologies, Inc. 2011-2012
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Manual Part Number
G1170-90001 Rev. B
Edition
05/2012
Printed in Germany
Agilent Technologies
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Agilent 1290 Infinity Valve Drive and Valve Heads 3
Contents
Contents
1 Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 7
Overview of the Valve Drive 8
Overview of the Agilent Quick Change Valve Heads 12
Typical Applications 13
Bio-inert Materials 21
Early Maintenance Feedback 23
2 Site Requirements and Specifications 25
Site Requirements 26
Physical Specifications 29
Performance Specifications 30
3 Installing the Module 33
Unpacking the Module 34
Installing the Valve Drive 36
Installing the Valve Heads 42
4 Using the Module 49
Hints for Successful Use 50
Solvent Information 52
Software Configuration 57
5 Troubleshooting and Diagnostics 61
Overview of the Module’s Indicators and Test Functions 62
Module Status Indicator 63
Agilent Lab Advisor Software 64
6 Error Information 65
What Are Error Messages 66
General Error Messages 67
Module Specific Error Messages 72

4 Agilent 1290 Infinity Valve Drive and Valve Heads
Contents
7 Maintenance 75
Introduction to Maintenance 76
Cautions and Warnings 77
Overview of Maintenance 79
Cleaning the Module 80
Replacing Parts of the Valve Head 81
Replacing the Fuses of the Infinity Valve Drive 83
Replacing Valve Heads 86
Replacing the Module Firmware 90
8 Parts and Materials 91
Valve Drive Parts 92
Valve Options Overview 94
Accessory Kit 96
Valve Products 97
Capillary Kits 98
Tubing Kit 106
2 Pos/6 Port Valve Head 600 bar 107
2 Pos/6 Port Ultra High Pressure Valve Head 1200 bar 108
2 Pos/6 Port Valve Head 600 bar (Bio-inert) 109
2 Pos/10 Port Micro Valve Head 600 bar 110
2 Pos/10 Port Ultra High Pressure Valve Head 1200 bar 111
8 Pos/9 Port Valve Head 600 bar 112
8 Pos/9 Port Ultra High Pressure Valve Head 1200 bar 113
Valve Head 4 Column Selector 600 bar (Bio-inert) 114
Valve Head 6 Column Selector 600 bar 115
Valve Head 6 Column Selector 1200 bar 116
12 Pos/13 Port Valve Head 210 bar (Bio-inert) 117
9 Identifying Cables 119
Cable Overview 120
Analog Cables 122
Remote Cables 124
BCD Cables 127
CAN/LAN Cables 129
External Contact Cable 130
Agilent Module to PC 131

Agilent 1290 Infinity Valve Drive and Valve Heads 5
Contents
10 Hardware Information 133
Electrical Connections 134
Interfaces 137
Config Switch Settings of the Infinity Valve Drive 144
11 Appendix 147
General Safety Information 148
The Waste Electrical and Electronic Equipment Directive 151
Radio Interference 152
Sound Emission 153
Agilent Technologies on Internet 154

6 Agilent 1290 Infinity Valve Drive and Valve Heads
Contents

7
Agilent 1290 Infinity Valve Drive and Valve Heads
Agilent Technologies
1
Introduction to the Agilent 1290 Infinity
Valve Drive and Valve Heads
Overview of the Valve Drive 8
Overview of the Agilent Quick Change Valve Heads 12
Typical Applications 13
Dual and Multi Column Selection 13
Sample Enrichment and Sample Cleanup 15
Alternating Column Regeneration 18
Solvent Selection 20
Bio-inert Materials 21
Early Maintenance Feedback 23
This chapter gives an introduction to the module, instrument overview and
internal connectors

8 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Overview of the Valve Drive
Overview of the Valve Drive
The Agilent 1290 Infinity Valve Drive (G1170A) is the latest addition to the
1290 Infinity LC system. This is a valve drive delivered without a specific valve
head. To be used in a HPLC system it is required to add a valve head to this
device. It is compatible with all currently available Agilent Quick Change Valve
heads (see Table 1 on page 12)
It includes the following features:
•Built-in power supply
•Leak handling with a leak pane and a leak sensor underneath the valve
head
•User exchangeable valve heads (Agilent Quick Change valve heads)
•Valve type and pressure detected by RF-ID tag
•Flexible mounting bracket, for left- or right-side mounting on LC stacks or
at the Agilent Column Organizer (G1383A).

Agilent 1290 Infinity Valve Drive and Valve Heads 9
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Overview of the Valve Drive
Figure 1 Mounting Examples for 1290 Infinity Valve Drive

10 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Overview of the Valve Drive
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Agilent 1290 Infinity Valve Drive and Valve Heads 11
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Overview of the Valve Drive
Figure 2 Rear View of 1290 Infinity Valve Drive
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12 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Overview of the Agilent Quick Change Valve Heads
Overview of the Agilent Quick Change Valve Heads
The Agilent Quick Change Valve Heads can be installed to the G1316C 1290
Infinity TCC, G4227A 1290 Infinity Flexible Cube and to the G1170A 1290
Infinity Valve Drive.
For bio-inert modules use bio-inert parts only!
Table 1 Overview of the available Agilent Quick Change Valve Kits
Valve Kit PN Pressure
[bar]
Positions Ports Typical Application
G4230A 600 8 9 Method development solution kits
G4230B 1200 8 9
G4231A 600 2 6 Any two-way switching, e.g.
between two detectors, between
waste and detector, between two
columns, for G4231B 1200 2 6
matrix-stripping, for
pre-concentration
G4231B 1200 2 6
G4232A-micro 600 2 10 Anything a 2pos/6port valve can
do plus alternating column
regeneration. The G4232A can be
used for standard as well as
cap/nano applications (uses
M4-fittings)
G4232B 1200 2 10
G4234A 600 6 14 4 to 6 column selection
G4234B 1200 6 14
G4235A 210 12 13 Solvent selection or fractionation
G5631A 600 2 6 Same as G4231A/B
G5639A 600 4 10 4 column selection

Agilent 1290 Infinity Valve Drive and Valve Heads 13
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Typical Applications
Typical Applications
The Infinity valve heads allow the user a variety of different applications. They
can either be installed to a Infinity valve drive or to a G1316C TCC that is
equipped with an internal valve actuator.
Examples of typical applications are:
•Dual and multiple column selection
•Sample enrichment and sample cleanup
•Alternating Column Regeneration
•Solvent selection
Dual and Multi Column Selection
Dual column selection
2pos/6port valve (p/n G4231A/B) and 2pos/10port valve (p/n G4232A/B)
Advantages:
•Increase productivity
•Higher instrument up-time
Quickly change between two different stationary phases to check your
separation selectivity, or use two identical stationary phases to have the
second column immediately available after the first one loses efficiency, for
example with complex matrices.

14 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Typical Applications
Multi Column Selection
With the 6 position selection valve (G4234A/B) and the capillary kit for
Column Selection you can set up your system for use with up to 6 columns as
displayed in Figure 3 on page 14. Or you can use the system with 5 columns
and one flow path for flow injection analysis or for flushing the system. This
setup allows you to switch between these columns for faster method
development or method validation. The multi-column setup might also be
used, if several operators are sharing system.
Figure 3 Multiple Column Selection (Example of Schematic Setup for 6 Column
Selector)

Agilent 1290 Infinity Valve Drive and Valve Heads 15
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Typical Applications
Sample Enrichment and Sample Cleanup
2pos/6port valve (p/n G4231A/B) and 2pos/10port valve (p/n G4232A/B)
Advantages:
•Easy automation of sample preparation
•Higher reproducibility
•Increased productivity and sensitivity
Sample cleanup is essential for samples with complex matrices, such as
biological fluids, food extracts and waste water. Before injection into a LC or
LC/MS system, the sample matrix must be separated from the analytes of
interest. Otherwise, contaminants can disrupt separation and detection or
even damage the analytical column.

16 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Typical Applications
Figure 4 Sample enrichment

Agilent 1290 Infinity Valve Drive and Valve Heads 17
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Typical Applications
Figure 5 Sample clean-up

18 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Typical Applications
Enrichment methods
Enrichment methods are the technique of choice to obtain highest sensitivity
and to remove the sample matrix in such applications as proteomics, drug
metabolism and environmental trace analysis. The analytes are retained and
concentrated onto the pre-column, while the sample matrix is passed to waste.
After the valve switch, a second pump backflushes the analytes out of the
pre-column onto the separation column. This allows injection of large volumes
onto the pre-column, significantly expanding sensitivity in the range of ten to
several thousands.
Stripping methods
Stripping methods handle analytes and matrices in the opposite way to
enrichment methods. Matrix components are retained on the pre-column
while the analytes pass through to the separation column. After the valve
switches, an additional pump backflushes the matrix components out of the
pre-column to waste, while the analytes are separated on the main column.
Backflushing prepares the pre-column for the next injection.
Alternating Column Regeneration
Only 2pos/10port valve (p/n G4232A/B)
Advantages:
•High sample throughput
•Increased productivity
•High efficiency
Alternating column regeneration is a convenient way to increase the sample
throughput. The Agilent 1200 Infinity Series 2 position/ 10 port valves can be
used to increase the efficiency in laboratories running large amounts of
samples. Gradient elution is a common technique for separation of complex
samples in liquid chromatography, which requires column regeneration before
the subsequent run is started. Using alternating column regeneration valuable
time for the analysis is saved. Core of the alternating column regeneration is
the Agilent 1200 Infinity Series 2 position / 10 port valves, which allows
simultaneous analysis on one column while a second identical column is
flushed and equilibrated.

Agilent 1290 Infinity Valve Drive and Valve Heads 19
Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads 1
Typical Applications
Figure 6 Alternating Column Regeneration

20 Agilent 1290 Infinity Valve Drive and Valve Heads
1Introduction to the Agilent 1290 Infinity Valve Drive and Valve Heads
Typical Applications
Figure 7 Alternate Column Regeneration (Time Scheme)
Solvent Selection
The 12pos/13port valve (p/n G4235A) can be used for solvent selection (flow
rate < 10 mL/min) as illustrated in Figure 8 on page 20. It offers automated
access to 12 different eluents.
Figure 8 Solvent Selection (Schematic Setup)