Ocean Optics PX-2 Owner's manual

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PX-2
Pulsed Xenon Light Source
Installation and Operation Instructions
Description
The PX-2 Pulsed Xenon Light Source is a high flash rate, short-arc xenon lamp for applications
involving absorbance, reflection, fluorescence and phosphorescence measurements, and especially for
measuring optically or thermally labile samples.
The PX-2 has an SMA 905 Connector that couples to Ocean Optics miniature fiber optic
spectrometers and accessories, including optical fibers, cuvette holders, probes and other sampling
optics.
The PX-2 operates at speeds up to 220 Hz, offers excellent pulse-to-pulse stability, and has two trigger
modes for software control of the flash rate. Any external TTL positive pulse can be used to trigger the
PX-2. When it is coupled to a spectrometer, you can easily synchronize operation of both the light and
detector.
Because it produces a pulsed signal, the PX-2 is less likely to contribute to solarization in optical fiber
assemblies, which can occur when fibers are illuminated with signals less than 260 nm.
For an example of a phosphorescence application using the PX-2 with an Ocean Optics spectrometer
and an HR4-BREAKOUT Box, see Configuring Ocean Optics Spectrometers for Phosphorescence
Measurements in the Engineering Documents at www.oceanoptics.com.

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Parts Included
The PX-2 ships with the following items:
PX-2 Pulsed Xenon Light Source
12 VDC power adapter
15-pin interface cable
Additional Accessories
The following are additional accessories available from Ocean Optics that you may need, depending
on your system set-up:
•USB2000/PX-2 adapter (USB-ADP-PX2)
•Replacement bulb (PX-2-B)
WARNING
The PX-2 produces ultraviolet radiation. Direct eye contact can cause permanent
eye damage. Always wear safety eyewear, and never look directly into the light
source.
Dangerous voltages are present in the PX-2. Do not operate the PX-2 without the
housing intact.
The SMA 905 Connector can get hot during operation. Allow the lamp to cool
after operation before handling.
Do not drop the instrument, as this may cause permanent damage to the unit.
Handle with care.
Connecting the PX-2
The set-up procedure differs, depending on the spectrometer to which you are connecting the PX-2.
Connecting to a USB2000/USB2000+/USB4000/HR2000
Spectrometer
The USB2000 and HR2000 Spectrometers have a 10-pin connector. The
USB2000+/USB4000/HR2000 have a 20-pin connector. For these spectrometers, you need to use the
USB-ADP-PX2 serial adapter.

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►Procedure
Follow the steps below to connect the PX-2 to a USB2000/HR2000 Spectrometer:
1. Remove the PX-2 from the product packaging.
2. Connect the USB-ADP-PX2 Adapter to the spectrometer’s accessory connector. For
USB2000+/USB4000/HR2000 Spectrometers with the 20-pin connector, skip the first column
of pins (A1 and A2 on the right) when plugging in the adapter.
3. Tighten the jackscrew to hold the adapter in place.
4. Connect the DB15 cable that came with your PX-2 to the USB-ADP-PX2 Adapter, then to
your PX-2 light source.
You have now connected the PX-2 for operation.
Connecting to Ocean Optics Spectrometers with a 30-pin
Connector
Our other spectrometers such as the NIRQuest, Maya, QE Pro and HR4000 use a 30-pin accessory
connector.
►Procedure
Follow the steps below to connect the PX-2 to spectrometer with a 30-pin accessory connector:
1. Remove the PX-2 from the product packaging.
2. Connect the HR4000-CBL-BB ribbon cable to the spectrometer’s 30-pin accessory connector.
3. Connect DB15 cable assembly to the spectrometer’s ribbon cable and the other end to the PX-
2’s 15-pin connector.
You have now connected the PX-2 for operation.
Connecting the PX-2 Using the Breakout Box
The Breakout Box (HR4-BREAKOUT) offers another option for connecting the PX-2 to our
spectrometers with the 30-pin connector. The Breakout Box is a passive module that separates the
signals from the 30-pin port of the spectrometer into an array of standard connectors and headers.
You’ll want to use the Breakout Box if you need multiple interfaces to your spectrometer. See the
Breakout Box Installation and Operation Instructions for information on using the Breakout Box.
Note
If you are connecting the Breakout Box to the USB4000 Spectrometer, you must first
attach the USB-ADP-BB adapter to the spectrometer.

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Configuring the PX-2
After setting up the PX-2, you must configure it to operate with the spectrometer and software.
►Procedure
1. Connect an optical fiber to the SMA 905 Connector on the front panel of the PX-2.
2. Turn the PX-2 on via the power switch on the rear of the unit.
3. Select Multiple or Single TTL mode by moving the toggle switch on the rear panel of PX-2.
You must also configure this mode in the operating software. See TTL Mode Information
below.
4. Download the latest version of your spectrometer operating software, if necessary.
Continue to the next section for controlling your PX-2 with OceanView software.
TTL Mode Information
The PX-2 features a TTL switch that provides two modes of operation: Multiple mode and Single
mode.
Multiple Mode
In Multiple mode, the flash timing (pulse rate) is determined by the spectrometer. The Continuous
strobe rate is either fixed or software controlled. See your spectrometer operating software manual for
more information.
In either case, you must alter the spectrometer’s integration time so that a constant number of flashes
are observed during each integration period.
When using Multiple mode, you must ensure that a constant number of flashes occurs for each
integration cycle. This is accomplished by setting the pulse rate and integration time, which is
controlled by the operating software.
Note
For SpectraSuite users, if you are using Multiple Mode you must enter the continuous
strobe delay. To do this, select Spectrometer | Spectrometer Features from the
SpectraSuite menu. Click on the Continuous Strobe tab and enter the continuous
strobe delay.

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Spectrometer Strobe Rate Adjustments
The table below shows the Continuous Strobe frequency (multiple strobe operation) for each type of
OOI spectrometer.
Spectrometer Strobe Rate Adjustment
USB2000 Fixed at 122Hz1
All other OOI Spectrometers Software-controlled 220Hz-1Hz2
1Fixed frequency when the USB2000 is programmed with the PX2 firmware
2The maximum frequency of the PX2; the spectrometer can actually run faster
Single Mode
In Single mode, a single lamp flash occurs during each integration period. You can modify the flash
rate by changing the integration period. Since the PX-2 has a maximum repetition rate of 220 Hz, the
minimum integration time allowed in Single mode is 5 milliseconds.
Controlling the PX-2 in OceanView with Single
Strobe
The Single Strobe control in OceanView is used to adjust the PX-2 lamp strobe timing.
Note
The following procedure can also be performed in OceanView using the Graph View.
Right-click on the spectrometer node and select New Output Control Single Strobe.
►Procedure
1. Add the Single Strobe control to the Main Controls tab of the Acquisition Controls. To do this,
click the Add/Remove Controls tab in the Acquisition Parameter Controls.

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2. Check the Single Strobe option.

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3. Click the Main Controls tab to go back to the Main Controls.
4. Set the PX-2 Flash Mode switch (on the back of the PX-2) to Single mode to flash the lamp
once per integration period.
5. Set the Holdoff Time (the time allowed before the pulse) and the Pulse Width in the Single
Strobe panel in the Acquisition Controls. See the data sheet for your spectrometer model for
pulse width limits.

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6. Click Enabled in the Single Strobe panel to pulse the lamp. (Make sure the lamp is turned
on.)
Controlling the PX-2 in OceanView with
Continuous Strobe
The Continuous Strobe control in OceanView is used to adjust the PX-2 lamp intensity per
integration period.
Note
The following procedure can also be performed in OceanView using the Graph View.
Right-click on the spectrometer node and select New Output Control Continuous
Strobe.

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►Procedure
1. Add the Continuous Strobe control to the Main Controls tab of the Acquisition Controls. To
do this, click the Add/Remove Controls tab in the Acquisition Parameter Controls.
2. Check the Continuous Strobe option.

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3. Click the Main Controls tab to go back to the Main Controls.
4. Set the PX-2 Flash Mode switch (on the back of the PX-2) to flash the lamp multiple times per
integration period.
5. Set the Strobe Period in the Continuous Strobe panel in the Acquisition Controls so that:
a. Strobe Period is equal to or smaller than the Integration Time
b. Strobe Period is a dividend/multiple of the Integration Time
c. Strobe Period is equal to or greater than 5 msec (5000 µsec). This applies only to the PX-2
lamp. Other pulsed lamps may have a faster repetition rate, enabling a lower value for the
strobe period.
Example:
•Set the Integration Time to 20 msec
•Set the Strobe Period to 20000 µsec
You can decrease Strobe Period to 10000 µsec or 5000 µsec to increase the repetition rate
of the lamp and increase the lamp intensity acquired per integration period.

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Note
If incorrect settings are used for the measurements, some spectra will be collected
when the lamp is flashing and others when the lamp is not flashing, or spectra may be
acquired with an inconsistent number of pulses per integration cycle. Improper
settings will cause variability in the results.
6. Click Enabled in the Continuous Strobe panel to pulse the lamp. (Make sure the lamp is
turned on.)

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PX-2 Specifications
Specification Value
Dimensions (mm) 153.4 x 104.9 x 40.9 mm
Weight 370 g
Power consumption 1.5 A @ 12 VDC
Power output 45 microjoules per pulse maximum 9.9 watts average power
220 Hz pulse rate maximum
Spectral range 220-750 nm
Pulse duration 5 microseconds (at 1/3 height of pulse)
Aperture 3 mm
Connector SMA 905
Lifetime 109pulses (estimated 230 days continuous operation)
PX-2 Pinout Information
The following section contains information on the 15-pin connector on the PX-2.
Pin Number Description
1 Single strobe
2 Continuous strobe
10 Ground

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PX-2 Information for S2000 Customers
The following information for the PX-2 light source applies only to customers with an S2000
Spectrometer.
Connecting to an S2000 Spectrometer
►Procedure
1. Remove the PX-2 from the product packaging.
2. Plug the 12 VDC power supply into a wall outlet, then connect the barrel connector on the
power supply to the rear of the PX-2.
3. Connect the 15-pin cable to the rear of the PX-2, and then connect the other end to the 15-pin
connector on the S2000 Spectrometer.
You have now connected the PX-2 for operation with the S2000 Spectrometer.
TTL Multiple Mode Information When Using an S2000
Spectrometer
The PX-2 features a TTL switch that provides two modes of operation: Multiple mode and Single
mode. In Multiple mode using an S2000 Spectrometer, flash timing is determined by the JP3 jumper
block setting. See your S2000 Spectrometer manual for more information.
S2000 Spectrometer Strobe Rate Adjustments
To achieve a constant number of flashes per integration cycle, the integration time must be a multiple
of those shown in the following table.
S2000 JP3
Setting
Integration time must be a multiple of (in milliseconds)
ADC500/SAD500 ADC1000 DAQ700 ADC2000-PCI
216 (factory
default) 128 64 512 32
214 32 16 128 8
212 8 4 32 2
210 N/A N/A 8
(with a minimum value
of 24 ms) N/A
The PX-2 repetition rate is determined by the JP3 setting on the S2000 Spectrometer, but it is also
dependent on the frequency of the A/D card. The table below shows the repetition rate for the various
combinations of hardware and jumper settings. The default factory setting is 216:

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JP3 Post # Function Frequency (Hz)
ADC500/SAD500 ADC1000 DAQ700 ADC2000-PCI
1 Not enabled – Reserved for future software control
2 Divide by 210 Too fast Too fast 98 Too fast
3 Divide by 212 122 Too fast 24 Too fast
4 Divide by 214 30 60 6.1 120
5 Divide by 216 7.6 15.2 1.5 30