Ocean Optics USB2000 Plus User manual

1.
USB2000+ Fiber Optic Spectrometer
Installation and Operation Manual
Document Number 270-00000-000-02-0108
Offices: Ocean Optics, Inc. World Headquarters
830 Douglas Ave., Dunedin, FL, USA 34698
Phone [phone]
Fax [phone]
8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST
E-mail: [email protected] (General sales inquiries)
[email protected] (Questions about orders)
[email protected] (Technical support)

Additional
Offices: Ocean Optics Asia
666 Gubei Road, Kirin Tower, Suite 601B, Changning District, Shanghai,
PRC. 200051
Phone [phone]
Fax [phone]
E-Mail [email protected]
Ocean Optics B.V. (Europe)
Geograaf 24, 6921 EW DUIVEN, The Netherlands
Phone [phone]
Fax [phone]
E-Mail [email protected]
Copyright © 2001-2007 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information
provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or
damages arising from the information contained in this manual.

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Table of Contents
About This Manual .......................................................................................................... iii
Document Purpose and Intended Audience.............................................................................. iii
Document Summary.................................................................................................................. iii
Product-Related Documentation ............................................................................................... iii
Upgrades......................................................................................................................... iv
Service ............................................................................................................................ iv
Chapter 1: Introduction ......................................................................1
Product Overview ............................................................................................................ 1
Features .......................................................................................................................... 2
System Requirements ..................................................................................................... 2
EEPROM Utilization .................................................................................................................. 2
About SpectraSuite.................................................................................................................... 2
Sampling System Overview....................................................................................................... 3
How Sampling Works............................................................................................................ 3
Interface Options ............................................................................................................. 3
Shipment Components.................................................................................................... 3
Additional Equipment Needed......................................................................................... 4
Other Accessories Available ........................................................................................... 4
Chapter 2: Installing the USB2000+...................................................6
Overview ......................................................................................................................... 6
USB2000+ Installation..................................................................................................... 6
USB Mode ................................................................................................................................. 6
Serial Port Mode........................................................................................................................ 7
Connect Spectroscopic Accessories ............................................................................... 7
USB2000+ Configuration................................................................................................. 7
Configuring the USB2000+ in SpectraSuite .............................................................................. 7
Configuring the USB2000+ in OOIBase32................................................................................ 8

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Chapter 3: Troubleshooting...............................................................9
Overview ......................................................................................................................... 9
USB2000+ Connected to Computer Prior to SpectraSuite Installation ........................... 9
Windows Operating Systems .................................................................................................... 9
Remove the Unknown Device from Windows Device Manager ........................................... 9
Remove Improperly Installed Files........................................................................................ 10
Mac Operating Systems ............................................................................................................ 10
Linux Operating Systems .......................................................................................................... 11
Appendix A: Calibrating the Wavelength of the USB2000+............13
Overview ......................................................................................................................... 13
About Wavelength Calibration......................................................................................... 13
Calibrating the Spectrometer........................................................................................... 14
Preparing for Calibration............................................................................................................ 14
Calibrating the Wavelength of the Spectrometer ...................................................................... 14
Saving the New Calibration Coefficients: USB Mode ...................................................... 16
Appendix B: External Triggering .......................................................17
Overview ......................................................................................................................... 17
Normal (Free Running).............................................................................................................. 17
External Software Trigger.......................................................................................................... 17
External Hardware Trigger ........................................................................................................ 17
External Synchronization Trigger .............................................................................................. 18
Appendix C: Specifications................................................................19
Overview ......................................................................................................................... 19
How the USB2000+ Works.............................................................................................. 19
USB2000+ Components Table.................................................................................................. 20
USB2000+ Specifications................................................................................................ 21
20-Pin Accessory Connector Pinout................................................................................ 22
20-Pin Accessory Connector Pinout Diagram........................................................................... 22
20-Pin Accessory Connector – Pin Definitions and Descriptions.............................................. 22
Index.....................................................................................................25

270-00000-000-02-0108 iii
About This Manual
Document Purpose and Intended Audience
This document provides the users of USB2000+ Spectrometers with instructions for setting up,
calibrating and performing experiments with their spectrometer.
Document Summary
Chapter Description
Chapter 1: Introduction Contains descriptive information about the USB2000+
Spectrometer and how sampling works. It also provides a list
of system requirements, interface options, and shipment
components.
Chapter 2: Installing the
USB2000+
Provides installation instructions.
Chapter 3: Troubleshooting Contains recommended steps to isolate and correct common
problems.
Appendix A: Calibrating the
Wavelength of the USB2000+
Provides instructions for calibrating the USB2000+
Spectrometer.
Appendix B: External Triggering Contains information about external triggering for the
USB2000+.
Appendix C: Specifications Contains technical specifications and connector pinouts for the
USB2000+ Spectrometer.
Product-Related Documentation
You can access documentation for Ocean Optics products by visiting our website at
http://www.oceanoptics.com. Select Technical →Operating Instructions, then choose the appropriate
document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.
•Detailed instructions for SpectraSuite Spectrometer Operating Software are located at:
http://www.oceanoptics.com/technical/SpectraSuite.pdf.
•Detailed instructions for the Breakout Box are located at:
http://www.oceanoptics.com/technical/HR4_breakout.pdf.
•Detailed instructions for external triggering are located at:
http://www.oceanoptics.com/technical/external-triggering.pdf.

About This Manual
iv 270-00000-000-02-0108
Engineering-level documentation is located on our website at Technical →Engineering Docs.
You can also access operating instructions for Ocean Optics products from the Software and Technical
Resources CD that ships with the product.
Upgrades
Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system.
To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise
Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a
product.
Service
Our Total Technical Service protection plans protect your spectrometer for one year for any contingency -
- no matter what the circumstances, including total loss. Under these plans, Ocean Optics will repair or
replace your instrument with no deductible or any other out-of-pocket expense required from you. USB
service plans include the following:
•TTS-USB: Protects your Ocean Optics USB spectrometer for any contingency for 1 year.
Provides for full spectrometer replacement, if necessary. Limited to 1 full replacement per year.
•TTS-USB-E: This special plan for Ocean Optics USB spectrometers not previously covered
protects for any contingency for 1 year. Provides for full spectrometer replacement, if necessary.
Limited to 1 full replacement per year. This coverage starts 30 days after purchasing the plan.
In addition, we also have the WAVECAL-USB spectrometer maintenance and calibration
service available to recalibrate, clean and perform any adjustments necessary on your USB2000+
Spectrometer.
Contact us to learn more about these great service plans.

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Chapter 1
Introduction
Product Overview
The USB2000+ Miniature Fiber Optic Spectrometer is a unique combination of technologies a powerful
2-MHz analog-to-digital (A/D) converter, programmable electronics, a 2048-element CCD-array detector,
and a high-speed USB 2.0 port. This innovative combination produces our fastest spectrometer yet and
provides resolution to 0.35 nm (FWHM). The USB2000+ allows users to capture and store a full
spectrum into memory every millisecond (that's 1,000 full spectra every second) when the spectrometer is
interfaced to a computer via a USB 2.0 port. The USB2000+ is perfect for chemical, biochemical and
other applications where fast reactions need to be monitored.
The USB2000+ Spectrometer connects to a computer via the USB port or serial port. When connected
through a USB 2.0 or 1.1, the spectrometer draws power from the host computer, eliminating the need for
an external power supply. The USB2000+, like all USB devices, can be controlled by our SpectraSuite
software, a completely modular, Java-based spectroscopy software platform that operates on Windows,
Macintosh and Linux operating systems.
Ocean Optics USB2000+ Fiber Optic Spectrometer Typical Set-up

1: Introduction
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Features
Sony ILX511 2048-element linear silicon CCD array detector
Responsive from 200 to 1100 nm
Sensitivity of up to 75 photons/count at 400 nm; 41 photons/count at 600 nm
An optical resolution of ~10.0 nm (FWHM)
Integration times from 3 ms to 65 seconds
EEPROM storage for
•Wavelength Calibration Coefficients
•Linearity Correction Coefficients
•Absolute Irradiance Calibration (optional)
Low power consumption of only 250 mA @ 5 VDC
16 bit, 3MHz A/D Converter
5 triggering modes
20-pin connector for interfacing to external products
CE Certification
RoHS compliant
System Requirements
You can use the USB2000+’s USB connectivity with any computer that meets the following
requirements:
•Operating system is one of the following:
•Windows – 98/Me/2000/XP
•Apple Macintosh – OS X version 10.0 or later
•Linux – Red Hat 9 or later, Fedora (any version), Debian 3.1 (Sarge), and SUSE (9.0 or
later)
•Ocean Optics’ SpectraSuite software application installed and configured for use.
EEPROM Utilization
An EEPROM memory chip in each USB2000+ contains wavelength calibration coefficients, linearity
coefficients, and a serial number unique to each individual spectrometer. The SpectraSuite software
application reads these values directly from the spectrometer, enabling the ability to “hot-swap”
spectrometers between computers without entering the spectrometer coefficients manually on each
computer.
About SpectraSuite
SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers. It is a
completely modular, Java-based spectroscopy software platform that operates on Windows, Macintosh
and Linux operating systems. The software can control any Ocean Optics USB spectrometer and device,
as well as any other manufacturer’s USB instrumentation using the appropriate drivers.

1: Introduction
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SpectraSuite is a user-customizable, advanced acquisition and display program that provides a real-time
interface to a variety of signal-processing functions. With SpectraSuite, you have the ability to perform
spectroscopic measurements (such as absorbance, reflectance, and emission), control all system
parameters, collect and display data in real time, and perform reference monitoring and time acquisition
experiments. Consult the SpectraSuite manual for hardware requirements when using SpectraSuite (see
Product-Related Documentation).
Sampling System Overview
How Sampling Works
Ocean Optics components function in a sampling system as follows:
1. The user stores reference and dark measurements to correct for instrument response variables.
2. The light from the light source transmits through an optical fiber to the sample.
3. The light interacts with the sample.
4. Another optical fiber collects and transmits the result of the interaction to the spectrometer.
5. The spectrometer measures the amount of light and transforms the data collected by the
spectrometer into digital information.
6. The spectrometer passes the sample information to SpectraSuite.
7. SpectraSuite compares the sample to the reference measurement and displays processed spectral
information.
Interface Options
The USB2000+ has both USB and serial port connectors (with the use of an adapter), enabling you to
connect the spectrometer to a computer via a USB or serial port. However, you must create custom
software if using the serial port. SpectraSuite software is available if you are connecting via the USB port.
Shipment Components
The following information and documentation ships with the USB2000+ Spectrometer:
Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, including customized components in the
spectrometer (such as the grating, detector collection lens, and slit). The packing list also includes
the shipping and billing addresses, as well as any items on back order.
USB Cable (USB-CBL-1)
Use this cable to connect your spectrometer to a computer running on a Windows, Mac or Linux
operating system.

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Wavelength Calibration Data Sheet
Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information
unique to your spectrometer. SpectraSuite Operating Software reads this calibration data from
your spectrometer when it interfaces to a computer via the USB port.
Note
Please save the Wavelength Calibration Data Sheet for future reference.
Software and Technical Resources CD
Each order ships with the Ocean Optics Software and Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and
spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view these
files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources
CD.
Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the Software and Technical
Resources CD package.
Additional Equipment Needed
SpectraSuite Operating Software
See About SpectraSuite for more information.
Other Accessories Available
Visit us at www.OceanOptics.com for a complete list of products available for all of your spectroscopy
needs.
Fibers
Light Sources
Integrated Sampling Systems
Cuvettes
Filter Holders

1: Introduction
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Chapter 2
Installing the USB2000+
Overview
You must install the SpectraSuite software application prior to connecting the USB2000+ Spectrometer to
the computer. The SpectraSuite software installation installs the drivers required for USB2000+
installation. If you do not install SpectraSuite first, the system will not properly recognize the USB2000+.
If you have already connected the USB2000+ to a computer running on a Windows platform prior to
installing SpectraSuite, consult Chapter 3: Troubleshooting for information on correcting a corrupt
USB2000+ installation.
Note the spectrometer(s) that you have installed are listed in the Data Sources pane.
USB2000+ Installation
This section contains instructions for connecting the USB2000+ via both USB and serial modes.
USB Mode
Note
The USB port on a computer can power up to five USB2000+ spectrometer channels.
Systems with more than five channels require a powered USB hub.
►Procedure
Follow the steps below to connect the USB2000+ to a computer via the USB port:
1. Install SpectraSuite on the destination computer.
2. Locate the USB cable (USB-CBL-1) provided with the USB2000+.
3. Insert the square end of the cable into the side of the USB2000+.
4. Insert the rectangular end of the cable into the USB port of the computer.
If you installed SpectraSuite prior to connecting the USB2000+, the SpectraSuite installs the USB2000+
drivers. If the drivers do not successfully install (or if you connected the USB2000+ to the computer
before installing SpectraSuite), consult Chapter 3: Troubleshooting.

2: Installing the USB2000+
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If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.
Note the spectrometer(s) that you have installed are listed in the Data Sources pane.
Once you install the software and hardware, and establish your sampling system, you are ready to take
measurements.
Serial Port Mode
To use the serial port capacity of the USB2000+ Spectrometer, the computer must be running a 32-bit
version of the Windows operating system.
►Procedure
Follow the steps below to connect the USB2000+ to the computer via serial port:
1. Connect the serial cable adapter block to the appropriate pins of the USB2000+’s 30-Pin
Accessory Connector.
2. Connect one end of the 9-pin serial cable to the adapter block on the USB2000+, and then
connect the other end to a serial port on the computer.
3. Note the number of the serial port (COM Port) to which you connected the USB2000+ (some
computers may not have numbered ports; handheld computers typically have only one serial
port).
4. Plug the 5 VDC external power supply into an outlet and connect it to the USB2000+.
Connect Spectroscopic Accessories
To find operating instructions for USB2000+-compatible products (such as light sources, sampling
chambers, and probes), consult the Software and Technical Resources CD or the Ocean Optics website at
http://www.oceanoptics.com/technical/operatinginstructions.asp.
USB2000+ Configuration
The USB2000+ can be used with either SpectraSuite or OOIBase32 software when connected to the USB
port. The configuration process differs, depending on whether you are running SpectraSuite or
OOIBase32 software.
Configuring the USB2000+ in SpectraSuite
If you have followed the previous steps and started SpectraSuite, the spectrometer is already acquiring
data. Even with no light in the spectrometer, there should be a dynamic trace displayed in the bottom of
the graph. If you allow light into the spectrometer, the graph trace should rise with increasing light
intensity. This means the software and hardware are correctly installed.

2: Installing the USB2000+
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Note the spectrometer(s) that you have installed are listed in the Data Sources pane.
Configuring the USB2000+ in OOIBase32
Once you install the USB2000+, you must configure OOIBase32’s Configure Spectrometer options so
that OOIBase32 recognizes the USB2000+ Spectrometer. Consult the OOIBase32 Spectrometer
Operating Software Operating Instructions for detailed instructions on configuring the spectrometer in
OOIBase32 (see Product-Related Documentation).

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Chapter 3
Troubleshooting
Overview
The following sections contain information on troubleshooting issues you may encounter when using the
USB2000+ Spectrometer.
USB2000+ Connected to Computer Prior to
SpectraSuite Installation
Windows Operating Systems
If you connected your Ocean Optics USB2000+ device to the computer prior to installing your Ocean
Optics software application on a Windows platform, you may encounter installation issues that you must
correct before your Ocean Optics device will operate properly.
Follow the applicable steps below to remove the incorrectly installed device, device driver, and
installation files.
Note
If these procedures do not correct your device driver problem, you must obtain the
Correcting Device Driver Issues document from the Ocean Optics website:
http://www.oceanoptics.com/technical/engineering/correctingdevicedriverissues.pdf.
Remove the Unknown Device from Windows Device Manager
►Procedure
1. Open Windows Device Manager. Consult the Windows operating instructions for your computer
for directions, if needed.
2. Locate the Other Devices option and expand the Other Devices selection by clicking on the "+"
sign to the immediate left.

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Note
Improperly installed USB devices can also appear under the Universal Serial Bus
Controller option. Be sure to check this location if you cannot locate the unknown device.
3. Locate the unknown device (marked with a large question mark). Right-click on the Unknown
Device listing and select the Uninstall or Remove option.
4. Click the OK button to continue. A warning box appears confirming the removal of the Unknown
Device. Click the OK button to confirm the device removal.
5. Disconnect the USB2000+ from your computer.
6. Locate the section in this chapter that is appropriate to your operating system and perform the
steps in the following Remove Improperly Installed Files section.
Remove Improperly Installed Files
►Procedure
1. Open Windows Explorer.
2. Navigate to the Windows | INF directory.
Note
If the INF directory is not visible, you must disable the Hide System Files and Folders
and Hide File Extensions for Known File Types options in Windows Folder Options.
Access Windows Folder Options from Windows Explorer, under the Tools | Folder
Options menu selection.
3. Delete the OOI_USB.INF in the INF directory. If your computer is running either the Windows
2000 or XP operating system, you must also delete the OOI_USB.PNF file in the INF directory.
4. Navigate to the Windows | System32 | Drivers directory.
5. Delete the EZUSB.SYS file.
6. Reinstall your Ocean Optics application and reboot the system when prompted.
7. Plug in the USB device.
The system is now able to locate and install the correct drivers for the USB device.
Mac Operating Systems
Since there are no device files for the USB2000+ Spectrometer in a Mac operating system, you should not
encounter any problems if you installed the spectrometer before the SpectraSuite software.

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Linux Operating Systems
For Linux operating systems, all you need to do is install the SpectraSuite software, then unplug and
replug in the spectrometer. Technically, the driver files for Linux simply give nonprivileged users
permission to use newly connected hardware. There isn’t any long-term harm to plugging in the device
before installing the software.

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AppendixA
Calibrating the Wavelength of
the USB2000+
Overview
This appendix describes how to calibrate the wavelength of your spectrometer. Though each spectrometer
is calibrated before it leaves Ocean Optics, the wavelength for all spectrometers will drift slightly as a
function of time and environmental conditions. Ocean Optics recommends periodically recalibrating the
USB2000+.
About Wavelength Calibration
You are going to be solving the following equation, which shows that the relationship between pixel
number and wavelength is a third-order polynomial:
λ
p= I+ C1 p+ C2 p2+ C3 p3
Where:
λ = the wavelength of pixel p
I= the wavelength of pixel 0
C1= the first coefficient (nm/pixel)
C2= the second coefficient (nm/pixel2)
C3= the third coefficient (nm/pixel3)
You will be calculating the value for Iand the three Cs.

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14 270-00000-000-02-0108
Calibrating the Spectrometer
Preparing for Calibration
To recalibrate the wavelength of your spectrometer, you need the following components:
•A light source capable of producing spectral lines
Note
Ocean Optics’ HG-1 Mercury-Argon lamp is ideal for recalibration. If you do not have an
HG-1, you need a light source that produces several (at least 4-6) spectral lines in the
wavelength region of your spectrometer.
•A USB2000+ spectrometer
•An optical fiber (for spectrometers without a built-in slit, a 50-µm fiber works best)
•A spreadsheet program (Excel or Quattro Pro, for example) or a calculator that performs third-
order linear regressions
Note
If you are using Microsoft Excel, choose Tools | Add-Ins and check AnalysisToolPak
and AnalysisTookPak-VBA.
Calibrating the Wavelength of the Spectrometer
►Procedure
Perform the steps below to calibrate the wavelength of the spectrometer:
1. Place SpectraSuite into Scope mode and take a spectrum of your light source. Adjust the
integration time (or the A/D conversion frequency) until there are several peaks on the screen that
are not off-scale.
2. Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum
intensity.
3. Record the pixel number that is displayed in the status bar or legend (located beneath the graph).
Repeat this step for all of the peaks in your spectrum.
4. Use the spreadsheet program or calculator to create a table like the one shown in the following
figure. In the first column, place the exact or true wavelength of the spectral lines that you used.
In the second column of this worksheet, place the observed pixel number. In the third column,
calculate the pixel number squared, and in the fourth column, calculate the pixel number cubed.