Ocean Optics LS-450 Series User manual

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 1
LS-450 Series Blue LED Pulsed Light Sources
The LS-450 Series Blue LED Pulsed Light Sources are compact, low-cost light-emitting diodes that produce
pulsed or continuous spectral output at 470 nm – the blue region – for high-sensitivity emission fluorescence
measurements.
The LS-450 light source can be combined with other sampling optics for a variety of fluorescence applications.
The LS-450 series consists of the following light sources:
• LS-450 (stand-alone version)
• R-LS-450 (rack-mount version)
• R-LS-450-2 (rack-mount version with 2 LEDs)
• USB-LS-450 (connects directly to USB spectrometers, documented separately)
LS-450 Stand-alone Version
Included Materials
• LS-450 light source
• DB-15 accessory cable
• Power supply
Connecting the LS-450
To connect the LS-450, follow the steps below:
1. Plug the wall transformer into a standard 110V outlet.
2. Connect the power cord to the power input on the LS-
450.
3. Connect an optical fiber to the SMA 905 connector on
the LS-450.
4. Connect the other end of the optical fiber to the SMA
905 connector on your spectrometer or sampling device.
Operation
To use the LS-450, connect the light source by following the instructions above, then proceed:
Using Continuous Mode
To operate the LS-450 in Continuous mode, follow the steps below:
1. Move the switch on the back of the LS-450 to the “contin” setting. This means that the light coming out of
the LS-450 is continuous.
2. Move the switch on the back of the LS-450 to the “off” setting to turn the lamp off.
LS-450 Blue LED Pulsed Light Source -
Stand-alone Version

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 2
Using Pulsed Mode
To operate the LS-450 in Pulsed mode, follow the steps below:
1. Connect one end of the DB-15 accessory connector to the back of the LS-450.
2. Connect the other end of the DB-15 accessory connector to the 15-pin connector or adapter on your
spectrometer. For more information, see the Pulsed Mode Connection Guide section later in this
document.
3. Move the switch on the back of the LS-450 to the “pulsed” setting to enable pulsed mode operation.
4. Configure Pulsed mode for your spectrometer. See the Pulsed Mode Connection Guide section later in
this document for more information.
5. Set the integration time in the OOIBase32 software so that a constant number of flashes occur for every
integration cycle. If you are using the S2000 spectrometer, you will need to set the integration time to
ensure a constant number of flashes per integration cycle. See the Pulsed Mode Connection Guide
section later in this document for a configuration table for the S2000 spectrometer.
See the OOIBase32 Spectrometer Operating Software Online Help System for more information on
setting the integration time.
Note: USB-based spectrometers automatically flash the lamp once per millisecond. Thus, there are
always a constant number of flashes regardless of the integration time configured.
Pulsed Mode Connection Guide
Follow the instructions below for your specific spectrometer to configure Pulsed mode on the LS-450:
S2000
1. Open the S2000 spectrometer housing and remove the spectrometer from the housing. Do not tamper
with the optical bench.
Note: If you have more than one channel in your system, you may have to disconnect the channels
from one another. The master spectrometer is always on the bottom of a multiple channel
system.
2. Locate Jumper Block 3 (labeled JP3) on the green circuit board. This jumper block consists of 10 pins,
which are labeled by rows (/16, /14, /12, /10, and 2).
3. Attach the jumper to the appropriate pins to configure the number of pulses per second for your A/D
converter. The table below illustrates the possible number of pulses per second for each A/D converter:
S2000 JP3 Pin Row ADC2000-PCI Frequency (Hz) ADC1000 Frequency (Hz)
/16 30.4 15.2
/14 120 60.8
/12 488 244.0
/10 1952 976.0
2 Controlled by flash delay in software
(Continued)

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 3
4. Ensure that there are a constant number of flashes for every integration time by adjusting the integration
time in OOIBase to a figure in the table below.
S2000 JP3 Pin Row ADC2000-PCI
Acceptable Int. Times ADC1000 Acceptable Int. Times
/16 32 64
/14 8 16
/12 2 4
/10 2 4
2 Integration time must be a multiple of flash delay
USB2000
1. Obtain and connect the USB-ADP-PX2 serial adapter to the 10-pin accessory connector on the USB2000
spectrometer.
2. Follow the instructions in the Using Pulse Mode section to connect the LS-450 to the spectrometer,
connecting the DB-15 to the USB-ADP-PX2.
3. Open OOIBase32 and configure the desired integration time.
4. Select the Strobe/Lamp Enable check box on the Acquisition Parameters toolbar.
When the Strobe/Lamp Enable option is selected in OOIBase, the USB2000 spectrometer will pulse the lamp at 1
msec intervals, ensuring a consistent pulse rate regardless of the configured integration time.
HR2000
1. Obtain and connect the USB-ADP-PX2 serial adapter to the 10 pins on the right side of the 20-pin
accessory connector on the HR2000 spectrometer.
Pinout Diagram of 20-pin Connector on
HR2000 Spectrometer
Note: It is important that you connect the serial adapter to the correct side of the HR2000. Pulsed
mode will not function unless this connection is accurate.
2. Follow the instructions in the Using Pulse Mode section to connect the LS-450 to the spectrometer,
connecting the DB-15 to the USB-ADP-PX2.
3. Open OOIBase32 and configure the desired integration time.
4. Select the Strobe/Lamp Enable check box on the Acquisition Parameters toolbar.
When the Strobe/Lamp Enable option is selected in OOIBase, the HR2000 spectrometer will pulse the lamp at 1
msec intervals, ensuring a consistent pulse rate regardless of the configured integration time.

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 4
R-LS-450 Rack-mount Blue LED Pulsed Light Sources
The R-LS-450 Blue LED Pulsed Light Source is the rack-
mount version of the LS-450. You can configure the R-LS-
450 to operate in continuous wave mode via manual
operation as well as through the software. You can also
configure the R-LS-450 to operate in pulsed mode through
manual operation and through the software.
Ocean Optics ships the R-LS-450 with following jumper
configuration:
• Jumper over pins in JP1
• Jumper over manual pins in JP3
• Jumper over 210 pins in JP2 (fastest pulse rate
available)
To modify these jumper settings, consult the Operation
section that follows.
Operation
You can configure the lamp using a switch and three jumper blocks on the circuit board of the R-LS-450 and, if
applicable, one jumper block on the S2000 spectrometer. The following section details the variety of
configurations available with the R-LS-450. You need to determine the best mode of operation for your setup and
configure your system appropriately.
S1 Switch
The S1 switch is a three-position switch on the R-LS-450. The switch can be moved into the following positions:
• Continuous wave operation
• Off
• Pulsed operation
Note: If you are using a USB-based spectrometer, instructions specifically for the S2000 spectrometer will not
apply.
Jumper Block 1 (JP1)
JP1 consists of only one set of pins. If you configure other jumper blocks correctly, a jumper over JP1 allows you
to turn the R-LS-450 on and off via the Strobe Enable/Disable feature in OOIBase32. This feature is only available
with a “J” or later version of the S2000 spectrometer (USB-based spectrometers have a fixed pulse rate of 1 ms).
Note: To find out if you have a J-series or later S2000, look at the third character in your S2000 serial number.
Jumper Block 2 (JP2)
JP2 consists of nine sets of pins. You can configure the number of pulses per seconds by changing which pins
are jumped in JP2. However, pulses per second are also dependent on the master frequency of the A/D converter
you are using.
The table on the following page contains information on the function of each set of pins in JP2.
R-LS-450-2 Dual Rack-mount Blue LED Pulsed
Light Source

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 5
• Jumping the CW pins makes the R-LS-450 operate continuously. This means that there is no pulsing of
the light source. Other jumper blocks must be configured correctly for this setting to work properly.
• Jumping sets 216, 215, 214, 213, 212, 211, and 210 controls the pulse rate per second of the R-LS-450,
depending on the A/D converter you are using to interface to the spectrometer (see the table below for
pulse rates).
• Jumping the CS pins controls the pulse rate via the spectrometer’s Continuous Strobe Setting in
OOIBase32 software (see the Using JP3 on the S2000 section that follows for more information).
Pins on JP2 Function ADC1000
Frequency (Hz) ADC2000-PCI Frequency (Hz)
CW Continuous mode 0 0
216 Divide by 216 15.2 30.4
215 Divide by 215 30.4 60.8
214 Divide by 214 60.8 122.0
213 Divide by 213 122.0 244.0
212 Divide by 212 244.0 488.0
211 Divide by 211 488.0 976.0
210 Divide by 210 976.0 1952.0
CS Continuous Strobe Software Controlled Software Controlled
Jumper Block 3 (JP3)
JP3 consists of two sets of pins. The jumper position determines the source of power control (manual or remote)
for the R-LS-450. Jumping the Remote pins allows you to control the R-LS-450 through the software (if other
jumper blocks are configured correctly).
R-LS-450 Operation Matrix
The following operation matrix will help you determine which jumper settings you require:
S1 Switch JP1 JP3 LED Status
Off No jumper No jumper Off
CW No jumper No jumper Continuously on
CW Jumpered Jumper Remote pins Continuous wave mode controlled by software
(See the Continuous Wave Mode with the
S2000’s JP3 section for more information)
CW Jumpered Jumper Manual pins Continuously on
Pulsed No jumper No jumper Pulse rate determined by JP2 on the R-LS-450
board (See the JP2 table for pulse rates)
Pulsed Jumpered Jumper Remote pins Pulse mode controlled by software (See the
Pulsed Mode with the S2000’s JP3 section for
more information)
Pulsed Jumpered Jumper Manual pins Pulse rate determined by JP2 on the R-LS-450
board (See the JP2 table for pulse rates)

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 6
Using Jumper Block 3 (JP3) on the S2000
You can also configure functionality on the R-LS-450 through JP3 on the S2000 circuit board (if you are using the
S2000 spectrometer). JP3 on the S2000 spectrometer allows you to control the R-LS-450 through the OOIBase32
software.
Continuous Wave Mode with JP3 on the S2000
You can turn the lamp of the R-LS-450 on and off (using JP3 of the S2000) while the unit is in continuous wave
mode using the Strobe Enable function in OOIBase32 ONLY if the following conditions are met:
• The switch is turned to continuous wave mode
• The pins in JP1 on the R-LS-450 are jumpered
• The CW pins in JP2 of the R-LS-450 are jumpered
• The Remote pins in JP3 of the R-LS-450 are jumpered
• The two pins in JP3 of the S2000 spectrometer are jumpered
Pulsed Mode with JP3 on the S2000
You can control the pulses per second of the R-LS-450 (using JP3 of the S2000) ONLY if the following conditions
are met:
• The switch is turned to pulsed mode
• The pins in JP1 of the R-LS-450 are jumpered
• The CS pins in JP2 of the R-LS-450 are jumpered
• The Remote pins in JP3 of the R-LS-450 are jumpered
• The pins labeled /16, /14, /12, or /10 (depending on the pulse rate you need) in JP3 of the S2000
spectrometer are jumpered.
The pulses per second are also dependent upon the frequency of your A/D converter. See the table below for
options.
S2000 JP3 Pin Row ADC2000-PCI Frequency (Hz) ADC1000 Frequency (Hz)
/16 30.4 15.2
/14 120 60.8
/12 488 244.0
/10 1952 976.0
2 Controlled by flash delay in software
You can control the pulses per second of the R-LS-450 through the Flash Delay function in the OOIBase32
software if the following conditions are met:
• The switch is turned to Pulse mode
• The pins in JP1 on the R-LS-450 are jumpered
• The CS pins in JP2 on the R-LS-450 are jumpered
• The Remote pins in JP3 on the R-LS-450 are jumpered
• The pins labeled “2” in JP3 on the S2000 spectrometer are jumpered

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 7
Setting the Integration Time with the R-LS-450 and the S2000
When using any of the pulsed modes and the R-LS-450, you must ensure that a constant number of flashes occur
for every integration cycle to ensure a continuous and stable signal. Set the integration time in the OOIBase32
software.
To achieve a constant number of flashes per integration cycle, the integration time you select must be a multiple
of the times shown in the table below (depending on the A/D converter you are using).
S2000 JP3 Pin Row ADC2000-PCI
Acceptable Int. Times ADC1000 Acceptable Int. Times
/16 32 64
/14 8 16
/12 2 4
/10 2 4
2 Integration time must be a multiple of flash delay
Note: USB-based spectrometers automatically flash the lamp once per millisecond. Thus, there are always a
constant number of flashes regardless of the integration time configured.
R-LS-450-2 Users
The instructions for controlling the R-LS-450-2 are exactly the same as those for the R-LS-450. The R-LS-450-2
has two LEDs, and the jumper configurations described in this manual will control both bulbs simultaneously.
There is no way to control each bulb individually.

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 8
USB-LS-450 Blue LED Pulsed Light Source
Functionally similar to the LS-450, the USB-LS-450 attaches directly to the 10-pin accessory connector of the
USB2000 Spectrometer. Its small-footprint design combines with the portability of the USB2000 to form a
complete, self-contained light source and spectrometer combination.
The USB-LS-450 features the following design innovations:
Simplified LED-Spectrometer Interface – USB-LS-450
connects to the USB2000 Spectrometer, which connects directly
to the USB port of any desktop or notebook PC.
Small Footprint – USB-LS-450 and spectrometer combination
is about the size of a deck and a half of playing cards.
On-board Memory for O2 Measurements – FOXY Fiber Optic
Oxygen Sensor users can conveniently store oxygen and
temperature calibration coefficients.
No External Power Requirements – Spectrometer and USB-
LS-450 draw power from the host PC.
Temperature Probe Connectivity – 4-pin connector on the
side of the USB-LS-450 allows quick interface of the USB-LS-
450-TP Temperature Probe used in the FOXY line of fiber optic
oxygen sensors.
USB-LS-450 Included Materials
• USB-LS-450 Blue LED Pulsed Light Source
• Allen wrench (to adjust collimating lens)
USB-LS-450 Setup
Follow the steps below to install the USB-LS-450:
1. Align the male 10-pin accessory input on the USB-LS-450 with the 10-pin accessory connector on the
USB2000 Spectrometer.
2. Secure the USB2000 and the USB-LS-450 together by firmly interfacing the 10-pin accessory connectors.
3. Insert the silver thumbscrew through the interface plate of the USB-LS-450 and tighten the thumbscrew to
secure the USB-LS-450 and the USB2000 together.
USB-LS-450 (bottom) connected to the
USB2000 Spectrometer

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 9
USB-LS-450 Specifications
USB-LS-450 LED Specifications
Item Specification
Power output: Minimum of 60 µW into a 600 µm optical fiber
LED drive current: 20 mA +/- 0.15 mA
Maximum modulation frequency: 1 kHz
0.5% stability time: Less than 1 minute
Temperature-dependent drift: +0.1%/°C
Temperature Measurement Specifications Power Specifications
Item Specification Item Specification
Precision: Better than 0.1 °C Input voltage range: 3.0 to 8.0 volts
Accuracy: Better than 0.5 °C Quiescent range: ~20 mA
Maximum Data Rate: 15 samples per second Current with lamp on: ~20 mA
USB-LS-450 Pin and Interface Information
The USB-LS-450 features a 10-pin accessory input (for the USB2000) and a
4-pin accessory connector (for the USB-LS-450-TP).
10-pin Accessory Input
The 10-pin accessory Input on the USB-LS-450 provides unaltered
connections to the 10-pin accessory connector on the USB2000
Spectrometer. Use this connector to configure external triggering options on
the USB2000 when the USB-LS-450 is connected to the spectrometer.
Consult the External Triggering Options document at
http://www.oceanoptics.com/technical/externaltriggering.pdf for more
information on specific pin information on the USB2000.
Note: If you have the 4/20mA (USB-LS450-4/20) output option, the 4-
20mA signal transmits on pin 8 of the 10-pin accessory input. The
ground transmits on pin 6.
4-pin USB-LS-450-TP Connector
The 4-pin USB-LS-450-TP connector is located on the side of the USB-LS-
450. It is a round connector approximately 1 cm in diameter. The USB-LS-
450-TP Temperature Probe plugs directly into this connector.
Consult the Fiber Optic Sensors Manual for more information on the USB-
LS-450-TP.
Front view of the USB-LS-450 with
10-pin accessory input
Pinout diagram of the 10-pin
connector on the USB2000
Side view of the USB-LS-450 with 4-
pin USB-LS-450-TP connector

LS-450 Series Blue LED Pulsed Light Sources
LS-450 Series Blue LED Pulsed Light Sources 10
LS-450 Series Technical Specifications
Lamp Specifications
LS-450, R-LS-450, R-LS-450-2 USB-LS-450
Physical dimensions: 90 mm x 50 mm x 32 mm
(LS-450 only) 89 mm x 102 mm x 38 mm
Power consumption: 25 mA @ 12 VDC 60 mA @ 5 VDC
Power output (minimum): 50 µW into 600 µm fiber 60 µW into 600 µm fiber
Stability: <1.0% after 2-minute warm-up <1.0% after 2-minute warm-up
LED drive current: 20 mA, 5 mA switchable 20 mA +/-0.15 mA
Absolute Spectral Output for LS-450 Blue LED Bulbs
Pinout Information
The following graphic and table illustrate the pinout information for the LS-450 light source:
15-pin connector on LS-450
Pin Number Description
2 Continuous strobe
10 Ground
This information applies only to the LS-450 model.