Olympus BX-FLA User manual

INSTRUCTIONS
BX-FLA
REFLECTEDLIGHT FLUORESCENCE
ATTACHMENT
This instruction manual is for the Olympus BX System Attachment Model BX-FLA. To ensure the
safety and obtain optimum performance and to familiarize yourself fully with the use of this attachment,
we recommend that you study this manual thoroughly before operating the microscope system.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
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AX5837

BX-FLA
This unit employs a UIS (universal infinity system) optical design, and should be used only with
UIS eyepieces, objectives, and condensers. Less than optimum performance may result if
inappropriate accessories are used.
L
J
The universal reflected light fluorescence vertical illuminator features interchangeable cubes to employ excitation
light of different wavelengths. If also allows combined or alternating reflected light fluorescence and transmitted
white light observations.
1. Reflected light fluorescence + transmitted light phase contrast.
2. Reflected light fluorescence + transmitted light Nomarski differential interference contrast.
3. Reflected light fluorescence + transmitted light brightfield or darkfield.
By mounting standard cubes for reflected light observation, the following observation methods become possible.
1. Reflected light brightfield/darkfield observation.
2. Reflected light Nomarski differential interference contrast.
3. Reflected light simple polarized light observation.
This instruction manual consist of two parts: I. Reflected Light Fluorescence Observation and II. Reflected
Light Observation Modes. Use these headings to find the relevant page with the instructions for the particular
observation mode.
a
Getting Ready
I. A vertical illuminator is delicate. Handle it carefully, and avoid jolts.
2. The high pressure mercury burner used in the unit should be a USH102 (mfd. by Ushio Electric). The
halogen lamp used in the unit should be a 12V, IOOWHAL halogen bulb (Philips 7724).
3. Verify that the burner is installed correctly and that all cords are properly connected.
4. The ultraviolet rays emitted by the burner are harmful. Be sure to use a UV protective shield with the unit.
(See page 9.)
5. Do not open the lamp housing while the burner is turned on or for at least 10 minutes after it is turned
off. The lamp housing inside is extremely hot and will cause injury if touched. (See page 11.)
6. The power supply unit contains high voltage components. Do not attempt to disassemble it.
7. Always ground the unit.
8. Before opening the lamp housing for replacement of the burner or other, turn off the main switch and
unplug the power output connector from the power unit. Wait for more than 10 minutes for the burner and
lamp socket to cool down.
9. Before plugging in the mains power cord, make sure that the main switch on the power supply unit is
turned off.
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with
gauze slightly moistened with a mixture of ether (70%) and alcohol (30%).
A Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be
sure to keep these chemicals away from open flames or potential sources of electrical sparks - for
example, electrical equipment that is being switched on or off. Also remember to always use these
chemicals only in a well-ventilated room.
2. Do not disassemble any part of the attachment.
3. The burner has a service life period of 200 hours. When the hour counter on the power supply unit indicates
200 hours, replace the burner with a new one. (See page 8.)
4. When not using the unit, cover it with the dust cover provided and store it in a dry place to prevent mold
formation.
5. If a dichroic mirror cube is not to be used for a while, place it in its container and store it in a safe place.

One additional intermediate tube can be used on the BX40 or BX50 together with the U-URA. Select an intermediate
tube to install on top of the vertical illuminator by referring to the illustration below.
Observation tube (U-SWTR, U-TR, etc.)
Eyepoint Magnification
adjuster changer
rsal reflected light
fluorescence vertical illuminator
U-URA
Conoscope
attachment
w
Microscope frame (BX40 or BX50)
+l
U-OPA
Orthoscope
attachment
Multi-viewing
attachment
U-DA
Drawing
attachment
Dual-viewing
attachment
If the equipment is used in a manner not specified by this manual, the safety of the equipment may be affected.
In addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

BX-FLA

The design of reflected light fluorescence microscopes features dichroic mirrors which direct the excitation light
through the objective, to the area of the specimen, thus providing efficient illumination. (Fig. 1)
Eyepiece
II
I
I
I
> Objective
I’
Specimen 4%
- Excitation light
-----+ Emitted light
Crossover
The spectral characteristics of the dichroic mirror when it is positioned
at an inclination of 45” to the optical axis of incident light is shown in
Fig. 2. Because a cross-over exists between transmittance and reflect-
ance, it is necessary to use an appropirate combination of exciter and
barner filters in conjunction with the dichroic mirror. This is necessary
to achieve a good contrast image through fluorochrome excitation in
the specimen, at the desired wavelength.
When the dichroic mirror is inclined 45” to the optical axis of incident
light, it reflects the excitation light towards the objective, and passes
unwanted wavelengths.
When the specimen is irradiated by the excitation wavelength, it emits
a visible longer wavelength corresponding to Stoke’s law. The barrier
filter mounted between the objective and eyepiece blocks out unwanted
wavelengths providing a black background.
1 0

Universal reflected light
fluorescence vertical illuminator
I I
Mercury lamp housing
Power supply unit
BX-series microscope
I
I
I
Universal condenser
0
2

1 A. Universal Reflected Light Fluorescence Vertical Illuminator
Light excluding
shutter
Filter slider
(Another is on opposite side.)
\\ Slider insertion slot
1 B. Cube
Removable index mark * Combine with dichroic mirrors, barrier filters and exciter filters
as appropriate for the desired excitation method.
Do not disassemble the cube.
3
4

) C. Fluorescent Light Source
l Mercury Burner Lamp Housing
Burner centering knob
Burner Socket
l Power Supply Unit
Burner ON LED
%Main switch
AC receptacle output
connector
0
4

0 To assemble the BX40 or BX50, consult the instruction manual pertaining to the BX40 or BX50 microscope.
k When assembling the units, make sure that all parts are free of dust and dirt, and assemble the parts
in the sequence indicated by the numbers in the illustration below.
Eyepiece
Observation tube
nrversal reflected
orescence vertic
UV protective shield
Light excluding slide
Microscope frame
*See paragraphmon page 16 regarding objectives to use for different observation methods.
+ For selection of the appropriate cubes for the observation, refer to Sectionnon page 17.
5
l

BX-FLA
Fig. 1
Fig. 2
Fig. 3
Fig. 4
a
Mounting the Cubes
{Figs. 1,2,31
0 See paragraph q on page 17 regarding which cubes to use for different
observation methods.
1. Loosen the clamping screw 0 at the right of the vertical illuminator
using the Allen screwdriver provided with the microscope frame.
(Fig. I)
2. Pull out the turret in the direction indicated by the arrow, then invert
the turret so that the cube dovetail mounts @ point upward.
(Dummy plates are mounted in three of the four cube positions. When
you wish to use only one cube, mount it in the empty position. When m A
using two or more cubes, loosen the clamping screw @ and remove
the dummy plate(s) by pulling in the direction indicated by the arrow, 5
and then mount the actual cube(s) instead.) Y
3. Hold the cube to be mounted with its index side facing upward and
slide it all the way onto the dovetail mount. Next, be sure to tighten
the cube clamping screw @Iimmediately. (Tighten all four cube clamping
screws.)
4. Remove the cube’s magnetic index sticker @ and affix it to the
corresponding turret position. (Fig. 2)
Ir Use a sharp object such as the tip of a ballpoint pen or
mechanical pencil to lift the cube’s magnetic index sticker.
Ir Move the cube’s magnetic index sticker (WU, NU, etc.) to the
corresponding A, B, C, D position on the turret. The positioning
indices @ A, B, C, and D on the cube dovetail mount corre-
spond to the A, B, C and D indices @ on the turret.
a Mounting thle Turret
(Fig. ‘1)
Insert the turret into the vertical illuminator housing and tighten the
lock screw @ while pushing the turret inward as far as it will go.
*When performing fluorescence observation, make sure to mount
dummy plates in the empty cube positions. For transmitted light
observation only, it is unnecessary to mount the dummy plates.
However, be sure to tighten the cube clamping screws when no
dummy plate is mounted. If the screws are left loose, the screw
heads may obstruct turret rotation. Mount the dummy plate by
reversing the procedure described in m-2.
Q
‘lVl-ousrti,~g.the Univqwl Fluorescenc;e
tirrEtrtic;afIlluimihatar (Fig 4)
Remove the two plugs from the top of the microscope frame and the
vertical illuminator. Then use the provided Allen screwdriver to clamp the
vertical illuminator to the microscope frame (two locations).
0
0 0