Olympus BX60 User manual

OfMMRUB
INSTRUCTIONS
BX60
SYSTEM MICROSCOPE
This instruction manual to for tho Olympus System Microscope Model BX60. To obtain optimum
performanca and to familiariie yourself fully whh the use of your microscope, we recommend
that you read this manual thoroughly before operating the microscope.

BX60
IMPORTANT
This unit employs the UIS (universal infinit system) optical design and should be used only with UIS
eyepieces, objectives, and condensers. Less than optimum performance may result if inappropriate
accessories are used.
BX6O is a versatile microscope which can be used both with transmitted light for use in the biological
field and with reflected light for metallurgical research.
Accordingly, this instruction manual contains:
• Separate outlines of the observation procedures when the microscope is used in biological or
metallurgical research purposes.
• Explanation of the use of controls is divided into sections.
• The most important points of the various observation methods are explained.
For reflected light fluorescence microscopy also refer to the manual pertaining to the reflected
light fluorescence attachment BX-fLA.
Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact
2. The 8X60 series can be used with any kind of one additional inter
mediate tube excluding U-DO dual-viewing attachment and U-MDO10
multi-viewing attachment.
3. Do not use the microscope where the following conditions exist: direct
sunlight high temperature and humidity, dust or vibrations. (Working
environment ambient temperature and hunrmfity should be in the range
of 0-40°C. 30-90%.)
4. When moving the microscope, carefully cany it with both hands by
grasping the arm as shown in the figure on the left-
* You can damage the microscope if It it lifted from the ttage.
coarse adjustment knob, or lamp housing.
5. Set the voltage selector switch on the rear of the base to the 100 -120V
or 220-240V position to match the local line voltage, using a flat-head
screwdriver. (Before shipment from the factory, the voltage selector
switch is set to 220-240V position). (See figure on the left)
6. To avoid potential shock hazard, be sure to properly ground the power
cord.
7. Always turn OFF the main switch and disconnect the power coed before
replacing the halogen bulb or fuses.

I
Maintenance and Storage;
1 Clean lenses by wiping gently with gauze. To remove fingerprints or oil stains, wipe with gauze slightty moistened
with xylene or a mixture of ether (70%) and alcohol 130%).
* SIneo ethar and alcohol are highly flammable, be careful to keep these chemicals away from an open fire
and potential sources of electrical sparks, such as main switches.
2. Do not attempt to use organic solvents to clean the microscope components especially plastic pans To dean use
a neutral detergent
3. Do not disassemble any pan of the microscope.
4. When not using the microscope, keep it covered with the provided dust cover.
Symbols on the Microscope Frame
Caution "...
If the microscope is operated in a manner not specified by this manual, the safety of the equipment may be
impaired. In addition, the equipment may also be damaged. Always operate the equipment as outlined in this
instruction manual.

,• M
BX60
CONTENTS
2-1 Assembly Diagram
2-2 Detailed Assembly Procedure.
Biological Microscopy
13
Metallurgical Microscopy
15
5-1 Base 17
5-2 Brightfield/Darkfield Vertical Illuminator 20
5-3 Stage 22
5-4 Observation Tube 25
5-5 Condenser 27
5-6 Focusing Adjustment Knobs 29
5-7 Immersion Objectives (for biological purposes only) 30
5-8 Photomicrography 30
!
6-1 Transmitted Light Brightfield Observation 32
6-2 Reflected Light Brightfield/Darkfield Observation 32
6-3 Reflected Light NcmarsH Differential Interference Contrast Observation 33
6-4 Reflected Light Simple Polarized Light Observation 34
6-5 Reflected Light Fluorescence Observation 35

NOMENCIA TVRE
1
u
.Eyepiece
Brightfield/darkfleld mirror
cuba housing or universal
I cube housing
Revolving
INosepiece
CondansarI
Observation tuba I
Brightflold/darkfieki
collector lens unit or
universal collector
lens unit
^\ Microsccpo frame |
Lamp Housing
for reflected light
Objective
Lamp housing for
transmitted light
Largo
stage
Inghi-r
1

BX60
ASSEMBLY
The diagram below shows how to assem&te the various components. The numbers indicate the order of assembly.
* When assembling the microscopo, make sura that all parts are free of dust and dirt and avoid scratching
any parts or touching glass surfaces.
Ump
housing
BriohtfWdMtrkfiald
Collector tans unit
IBrightfldd/dark-
ftakf or unhmsal)
Mtchtniol nag*
with WWund
(right-hind) low driv*
co»xl»l controls ,
Largi nMchtnlctl
sum with l«fl-h«nd
(right-hand) low (Irhw
coaxial controls
* The DF (dsrkfield) insert is attached when the microscopo is used for reflected light microscopy. Be
sure to remove the Insert when the microscope is used for transmitted light microscopy.

Attaching the Stage (U-SIC4, U-SV)
,Rg.,,
1. FuUy loosen the clamping screw © at the rear of the stage (at the
front n the case of U-SV).
2.
then tighten the clamping screw.
fig. 1
Rg.2
Mounting the Condenser )FIa. a
1. Turn the coarse adjustment knob © to raise the stage to its upper
limit
lAssuming there are no objectives mounted on the nosepiece yet.)
2. Tumiheco^r^heiBht
holder to its tower Emit
3. Loosen the condenser clamping screw.
4. Position the condenser with the scale markings in front and insert it
into the substage fork as far as it wB go.
5. Tighten the condenser clamping screw, then raise the condenser to
its upper limit
in tha substage fork.
™Ullin9 *• U'SC «•**•<"« «chrom«le condenser or the
U-UCO universal condenser, swing the top lens out of the way
before inserting the condenser.

BX60
Fig. 3
fio-4
Mounting tKS
(Universal COBe Housing)-
. (Rg*. 3,*)
O The following procedure does not apply to the briflhfieMWartfield mirror
cube housing.
* Loosen the screw that secures tha cube housing to the frame
with tha provided Allan screwdriver. It is located In the hole at
the right side of the arm.
1. Invert the cube housing so that the cube dovetail mounts on the turret
@ point upward.
(Dummy plates are mounted in three of the four cube positions. When
you wish to use only one cube, mount it in tha empty position. When
using tw6 or more cubes, loosen the clamping screw <D and remove
the dummy ptatels) by puffing in the direction infested by the arrow,
and then mount tha actual eubslsl in its place.)
2. Hold tha cube to be mounted with its index side facing upward and
slide it all the way onto the dovetail mount Next be sure to tighten
the cube damping screw © immediately. (Tighten all (our cube damp
ing screws.)
3. Remove the cube's magnetic index sticker rj>* end affix it to the corre
sponding turret position. (Fig. 4)
*U» a sharp abject such as the tip of a ballpoint pan or macfiinical pared to
lift tha Cuba's mfignatic indax sockar.
* The cube indices A, B.C. D® on the dovetail mount correspond
to the turret's A, B, C, D Indices (JX Make sure to match tha
attached cube correctly with the position of the removed magnetic
index sticker on the turret (Fig. 5)
Mounting the Cub« Housing.
(Ro,«
1. Align the cube housing dovetail © with the dovetai)® at the front of
the arm and fully slide it into tha arm.
2. insert the Allen screwdriver through the hole ® on the right side of
the arm. then securely tighten the cube housing damping screw.
\
Fig. 8

Mounting the Collector Lens Unit
(Fig. 6)
1. Gently insert the collector lens unit as far as it will go into the opening
located at the rear of the arm.
2. Insert the provided screws in the four screw holes ana tighten securery
with the Allen screwdriver
Fig. 6
Attaching the Field Iris Diaphragm
and Aperture Iris Diaphragm Knobs
(Rg.7»
Rg.7
1. Insert the provided field iris diaphragm and aperture iris diaphragm
knobs through (he holes 0 on the right side of the aim. then screw
in the knobs until tight
O When using the LMJflBL universal vertical illuminator lens unit the
shutter knob should also be attached in the same manner.
2. Insert the provided field iris diaphragm and aperture iris diaphragm
centering knobs through the holes (D on both sides of the arm. then
screw in the knobs until light.
3. Insert the provided pinhda knob through the hole on the left side of
the arm, then screw in the knob until tight (U-RLBL only).

-j
BX60
Fig. B
fig. 9
Installing therHalogen Bulb (Rga. B,
(for both Transmitted/Reflected Ughts) 9.10)
O The appropriate bulb is a 12V. 100WHAL halogen bulb (PhBips 77241.
1. Futry loosen the lamp housing damping screw© on top of the lamp
housing cover with the provided AQen screwdriver.
2. Lift the lamp housing cover Q) upward to remove it (fig. 81
3. Turn the lamp socket Q) 90* in the direction indicated by the arrow.
4. Holdtng the bulb® with gloves or a piece of gauze, depress the bulb
damping levers ® and insert the bulb pins ® MV into the pin holes
ffl. Gently release the bub damping levers ® to their original positions
to secure the bulb. (figs. 9. 10)
* Do not touch the bulb with bare hands. If fingerprints are
accidentally left on the bulb, wipe the bulb with toft doth.
S. Slide the lamp housing cover onto the housing base from above.
Tighten the damping screw ® whle pressing downward on the cover.
(Fig. 3)
* Whenever you replace the bulb, first tum OFF the main switch
and wait for ths bulb and lamp socket to cool down.
fig. 10