Olympus Bx60 User manual

OfMMRUB

INSTRUCTIONS

BX60

SYSTEM

MICROSCOPE

This

instruction

manual

to

for

tho

Olympus

System

Microscope

Model

BX60.

To

obtain

optimum

performanca

and

to

familiariie

yourself

fully

whh

the

use

of

your

microscope,

we

recommend

that

you

read

this

manual

thoroughly

before

operating

the

microscope.

BX60

IMPORTANT

This

unit

employs

the

UIS

(universal

infinit

system)

optical

design

and

should

be

used

only

with

UIS

eyepieces,

objectives,

and

condensers.

Less

than

optimum

performance

may

result

if

inappropriate

accessories

are

used.

BX6O

is

a

versatile

microscope

which

can

be

used

both with

transmitted

light

for

use

in

the

biological

field

and

with

reflected

light

for

metallurgical

research.

Accordingly,

this

instruction

manual

contains:

•

Separate

outlines

of

the

observation

procedures

when

the

microscope

is

used

in

biological

or

metallurgical

research

purposes.

•

Explanation

of

the

use

of

controls

is

divided

into

sections.

•

The

most

important

points

of

the

various

observation

methods

are

explained.

For

reflected

light

fluorescence

microscopy

also

refer

to

the

manual

pertaining

to

the

reflected

light

fluorescence

attachment

BX-fLA.

Getting

Ready

1.

A

microscope

is

a

precision

instrument.

Handle

it

with

care

and

avoid

subjecting

it

to

sudden

or

severe

impact

2.

The

8X60

series

can be

used

with

any

kind

of

one

additional

inter

mediate

tube

excluding

U-DO

dual-viewing

attachment

and

U-MDO10

multi-viewing

attachment.

3.

Do

not

use

the

microscope

where

the

following

conditions

exist:

direct

sunlight

high

temperature

and

humidity,

dust

or

vibrations.

(Working

environment

ambient

temperature

and

hunrmfity

should

be

in

the

range

of

0-40°C.

30-90%.)

4.

When

moving

the

microscope,

carefully

cany

it

with

both

hands

by

grasping

the

arm

as

shown

in

the

figure

on

the

left-

*

You

can

damage

the

microscope

if

It

it

lifted

from

the

ttage.

coarse

adjustment

knob,

or

lamp

housing.

5.

Set

the

voltage

selector

switch

on

the

rear

of

the

base

to

the

100

-120V

or

220-240V

position

to

match

the

local

line

voltage,

using

a

flat-head

screwdriver.

(Before

shipment

from

the

factory,

the

voltage

selector

switch

is

set

to

220-240V

position).

(See

figure

on

the

left)

6.

To

avoid

potential

shock

hazard,

be

sure

to

properly

ground

the

power

cord.

7.

Always

turn

OFF

the

main

switch

and

disconnect

the

power

coed

before

replacing

the

halogen

bulb or

fuses.

I

Maintenance

and

Storage;

1

Clean

lenses

by

wiping

gently

with

gauze.

To

remove

fingerprints

or

oil

stains,

wipe

with

gauze

slightty

moistened

with

xylene

or

a

mixture

of

ether

(70%)

and

alcohol

130%).

*

SIneo

ethar

and

alcohol

are

highly

flammable,

be

careful

to

keep

these

chemicals

away

from

an

open

fire

and

potential

sources

of

electrical

sparks,

such

as

main

switches.

2.

Do

not

attempt

to

use

organic

solvents

to

clean

the

microscope

components

especially

plastic

pans

To

dean

use

a

neutral

detergent

3.

Do

not

disassemble

any

pan

of

the

microscope.

4.

When

not

using

the

microscope,

keep

it

covered

with

the

provided

dust

cover.

Symbols

on

the

Microscope

Frame

Caution

"...

If

the

microscope

is

operated

in

a

manner

not

specified

by

this

manual,

the

safety

of

the

equipment

may

be

impaired.

In

addition,

the

equipment

may

also

be

damaged.

Always

operate

the

equipment

as

outlined

in

this

instruction

manual.

,•

M

BX60

CONTENTS

2-1

Assembly

Diagram

2-2

Detailed

Assembly

Procedure.

Biological

Microscopy

13

Metallurgical

Microscopy

15

5-1

Base

17

5-2

Brightfield/Darkfield

Vertical

Illuminator

20

5-3

Stage

22

5-4

Observation

Tube

25

5-5

Condenser

27

5-6

Focusing

Adjustment

Knobs

29

5-7

Immersion

Objectives

(for

biological

purposes

only)

30

5-8

Photomicrography

30

!

6-1

Transmitted

Light

Brightfield

Observation

32

6-2

Reflected

Light

Brightfield/Darkfield

Observation

32

6-3

Reflected

Light

NcmarsH

Differential

Interference

Contrast

Observation

33

6-4

Reflected

Light

Simple

Polarized

Light

Observation

34

6-5

Reflected

Light

Fluorescence

Observation

35

NOMENCIA

TVRE

1

u

.Eyepiece

Brightfield/darkfleld

mirror

cuba

housing

or

universal

I

cube

housing

Revolving

INosepiece

CondansarI

Observation

tuba

I

Brightflold/darkfieki

collector

lens

unit

or

universal

collector

lens

unit

^\

Microsccpo

frame

|

Lamp

Housing

for

reflected

light

Objective

Lamp

housing

for

transmitted

light

Largo

stage

Inghi-r

1

BX60

ASSEMBLY

The

diagram

below

shows

how

to

assem&te

the

various

components.

The

numbers

indicate

the

order

of

assembly.

*

When

assembling

the

microscopo,

make

sura

that

all

parts

are

free

of dust

and

dirt

and

avoid

scratching

any

parts

or

touching

glass

surfaces.

Ump

housing

BriohtfWdMtrkfiald

Collector

tans

unit

IBrightfldd/dark-

ftakf

or

unhmsal)

Mtchtniol

nag*

with

WWund

(right-hind)

low

driv*

co»xl»l

controls

,

Largi

nMchtnlctl

sum

with

l«fl-h«nd

(right-hand)

low

(Irhw

coaxial

controls

*

The

DF

(dsrkfield)

insert

is

attached

when

the

microscopo

is

used

for

reflected

light

microscopy.

Be

sure

to

remove

the

Insert

when

the

microscope

is

used

for

transmitted

light

microscopy.

Attaching

the

Stage

(U-SIC4,

U-SV)

,Rg.,,

1.

FuUy

loosen

the

clamping

screw

©

at

the

rear of

the

stage

(at

the

front

n

the

case

of

U-SV).

2.

then

tighten

the

clamping

screw.

fig.

1

Rg.2

Mounting

the

Condenser

)FIa.

a

1.

Turn

the

coarse

adjustment

knob

©

to

raise

the

stage

to

its

upper

limit

lAssuming

there

are

no

objectives

mounted

on

the

nosepiece

yet.)

2.

Tumiheco^r^heiBht

holder

to

its

tower

Emit

3.

Loosen

the

condenser

clamping

screw.

4.

Position

the

condenser

with

the

scale

markings

in

front

and

insert

it

into

the

substage

fork

as

far

as

it

wB

go.

5.

Tighten

the

condenser

clamping

screw,

then

raise

the

condenser

to

its

upper

limit

in

tha

substage

fork.

™Ullin9

*•

U'SC

«•**•<"«

«chrom«le

condenser

or

the

U-UCO

universal

condenser,

swing

the

top

lens

out

of

the

way

before

inserting

the

condenser.

BX60

Fig.

3

fio-4

Mounting

tKS

(Universal

COBe

Housing)-

.

(Rg*.

3,*)

O

The

following

procedure

does

not

apply

to

the

briflhfieMWartfield

mirror

cube

housing.

*

Loosen

the

screw

that

secures

tha

cube

housing

to

the

frame

with

tha

provided

Allan

screwdriver.

It

is

located

In

the

hole

at

the

right

side of

the

arm.

1.

Invert

the

cube

housing

so

that

the

cube

dovetail

mounts

on

the

turret

@

point

upward.

(Dummy

plates

are

mounted

in

three

of

the

four

cube

positions.

When

you

wish

to

use

only

one

cube,

mount

it

in

tha

empty

position.

When

using

tw6

or

more

cubes,

loosen

the

clamping

screw

<D

and

remove

the

dummy

ptatels)

by

puffing

in

the

direction

infested

by

the

arrow,

and

then

mount

tha

actual

eubslsl

in

its

place.)

2.

Hold

tha

cube

to

be

mounted

with

its

index

side

facing

upward and

slide

it

all

the

way

onto

the

dovetail

mount

Next be

sure

to

tighten

the

cube

damping

screw

©

immediately.

(Tighten

all

(our

cube

damp

ing

screws.)

3.

Remove

the

cube's

magnetic

index

sticker

rj>*

end

affix

it

to

the

corre

sponding

turret

position.

(Fig.

4)

*U»

a

sharp

abject

such

as

the

tip

of

a

ballpoint

pan

or

macfiinical

pared

to

lift

tha

Cuba's

mfignatic

indax

sockar.

*

The

cube

indices

A, B.C.

D®

on

the

dovetail

mount

correspond

to

the

turret's

A,

B,

C,

D

Indices

(JX

Make

sure

to

match

tha

attached

cube

correctly

with

the

position

of

the

removed

magnetic

index

sticker

on

the

turret

(Fig.

5)

Mounting

the

Cub«

Housing.

(Ro,«

1.

Align

the

cube

housing

dovetail

©

with

the

dovetai)®

at

the

front

of

the

arm and

fully

slide

it

into

tha

arm.

2.

insert

the

Allen

screwdriver

through

the

hole

®

on

the

right

side of

the

arm.

then

securely

tighten

the

cube

housing

damping

screw.

\

Fig.

8

Mounting

the

Collector

Lens

Unit

(Fig.

6)

1.

Gently

insert

the

collector

lens

unit

as

far

as

it

will

go

into

the

opening

located

at

the

rear of

the

arm.

2.

Insert

the

provided

screws

in

the

four

screw

holes

ana

tighten

securery

with

the

Allen

screwdriver

Fig.

6

Attaching

the

Field

Iris

Diaphragm

and

Aperture

Iris

Diaphragm

Knobs

(Rg.7»

Rg.7

1.

Insert

the

provided

field

iris

diaphragm

and

aperture

iris

diaphragm

knobs

through

(he

holes

0

on

the

right

side

of

the

aim.

then

screw

in

the

knobs

until

tight

O

When

using

the

LMJflBL

universal

vertical

illuminator

lens

unit

the

shutter

knob

should

also

be

attached

in

the

same

manner.

2.

Insert

the

provided

field

iris

diaphragm

and

aperture

iris

diaphragm

centering

knobs

through

the

holes

(D

on

both

sides

of

the

arm.

then

screw

in

the

knobs

until

light.

3.

Insert

the

provided

pinhda

knob

through

the

hole

on

the

left

side

of

the

arm, then

screw

in

the

knob

until

tight

(U-RLBL

only).

-j

BX60

Fig.

B

fig.

9

Installing

therHalogen

Bulb

(Rga.

B,

(for

both

Transmitted/Reflected

Ughts)

9.10)

O

The

appropriate

bulb

is

a

12V.

100WHAL

halogen

bulb

(PhBips

77241.

1.

Futry

loosen

the

lamp

housing

damping

screw©

on

top

of

the

lamp

housing

cover

with

the

provided

AQen

screwdriver.

2.

Lift

the

lamp

housing cover

Q) upward

to

remove

it

(fig.

81

3.

Turn

the

lamp

socket

Q)

90*

in

the

direction

indicated

by

the

arrow.

4.

Holdtng

the

bulb®

with

gloves

or

a

piece

of

gauze,

depress

the

bulb

damping

levers

®

and

insert

the

bulb

pins

®

MV

into

the

holes

ffl.

Gently

release

the

bub

damping

levers

®

to

their

original

positions

to

secure

the

bulb.

(figs.

9.

10)

*

Do

not

touch

the

bulb

with

bare

hands.

If

fingerprints

are

accidentally

left

on

the

bulb,

wipe

the

bulb

with

toft

doth.

S.

Slide

the

lamp

housing cover onto

the

housing

base from

above.

Tighten

the

damping

screw

®

whle

pressing

downward

on

the

cover.

(Fig.

3)

*

Whenever

you

replace

the

bulb,

first

tum

OFF

the

main

switch

and

wait

for

ths

bulb

and

lamp

socket

to

cool

down.

fig.

10

Attaching

the

Lamp

Housing

to

the

Microscope

|

Transmitted

Light

Lamp

Homing

|

(Figs.

11.

12.13)

fig,

11

1.

Using

the

Allen

screwdriver,

fully

loosen

the

lamp

housing

clamping

screw

©

on

the

microscope.

2.

Insert

the

lamp

housing

eoUactor

unit

mto

the

microscope

unU

it

touches

tha

lamp

housing

bracket

on

the

back

of

tha

base

(lower

part),

then

tighten

the

clamping

screw

©

(Fig.

11)

Rg.

12

|

Reflected

Light

Lamp

Homing

|

1.

Using

the

Allen

screwdriver,

fully

loosen

the

lamp

housing

damping

screw

©

on

the

reflected

Tight

port

on

the

microscope.

(Fig.

12)

2.

Insert

the

lamp

housing

collector

unit

into

the

lamp

port

unfl

it

touches

the

lamp

housing

bracket

on

the

back

of

the

base

(upper

pan),

than

tighten

the

clamping

screw

®.

(Fig.

13)

3.

Insert

the

cord plug

from

the transmitted

light

lamp

housing

securely

into

tha

power

outlet

©.

Insert

the

cord

plug

from

tha

reflected

light

lamp

housing

securely

into

the

power

outlet

©.

IFig.

13)

Fig.

13

Fig.

14

Mounting

the

Objectives

(Fig.

14)

C

For

reflected

tght

observation,

make

sure

to

mount

metallurgical

UIS

objectives.

Mount

the

objectives

on

the

revolving

nosepiece

in

such

a

manner

lhat

the

magnification

increases

from

tow

to

high

power

in

a

clockwise

direction.

(Fig.

14)

BX60

Mounting

tfie

Revolving

Noseplece*

*

(Rg.15)

*

Whan

used

for

metallurgical

obaarvatien:

Mount

tha

DF

insert

©.

The

insert

will

be

held

in

place

by

the

magnet on

tha

underside

of

the

arm.

1.

Turn

tha

coarse

adjustment

knob

to

lower

the

stage

all

the

way.

2.

Using

the

Allen

screwdriver,

loosen

the

nosaptece

clamping

screw

@

on

the

microscope.

3.

Carefully

slide

the

nosepisce

along

the

dovatail,

in

the

direction

of

the

arrow,

all

the

way

in

4.

Clamp

the

nosepiece

by

tightening

the

nosepiace

clamping

screw.

Mounting

the

Observation

Tuber

*

r

"

{Rg.

is>

1.

Using

ihe

ASen

screwdriver,

loosen

the

observation

tube

damping

screw

©■

2.

Insert

the

circular

dovetail

mount

at

the

bottom

of

the

observation

tube

into

the

opening

on

the

microscope

frame,

placing

the

observation

tube

to

point

the

binocular

eyepieces

towards

the front

Fasten the

observation

tube

by

tightening

the

clamping

screw.

Mounting

the

Eyepieces;-/

(Fig*

17,18)

go.

(Fig.

17)

Whan

Using

the

Trinocular

Observation

Tube

(U-TR30)

or

the

Supar-widaflold

Trlnocular

Observation

Tuba

IU-SWTR)

When

using

a

finder

eyepiece

or

an

eyepiece

with

irtci

xijustn

insert

this

eyepiece

into

the

right-hand

eyepiece

sleeve.

When

doing

so.

make

sure

that

the

eyepiece

positioning

<j)

fits

into

the

notch

®

at

the

bottom

of

tha

eyepiece

sleeve.

(Fig.

18)

8

I

Fig.

19

Rg.

20

Connecting

the

Power

Cord

(R9.

1.

Make

sure

that

the

main

switch

(?)

a

on

OFF.

2.

Before

shipment

from

the

factory,

the

voltage

selector

switch

fj)

u

set

to the

200-240V

position.

In

C33e

your

local

line

vortaga

is

10O-120V,

move

the

switch

to

tha

100-120V

position

using

a

flat-head

screwdriver.

3.

Insen

the

power

cord

into

the

AC

receptacle

®

on

the

frame.

4.

Connect

tha

power

cord's

ground

wire

to

the

ground

terminal

on

the

wall

outlet

then

plug

the

power

cord

into

the

wall

outlet,

(in

case

of

a

three-prong

power

cord

and

wall

outlet separate

grounding

is

not

necessary.)

Fuse

Replacement

(Fig*.

20, 21)

O

Before

replacing

fuses,

set

tha

main

switch

to

OFF

and

unplug

the

power

cord,

(the

power

cord should

be

unptugged from

the

AC

recepocle

to

allow

removal

of

the

fuse

holder.)

1.

Remove

the

fuse

holder

(t)

by

squeezing

it

at

both

sides

and

puling

outward.

fFtg.

20)

2.

Replace

both

fuse3

©

with

new

ones.

{Fig.

21)

*

Use

only

specified

fuses.

Applicable

fuse:

260V,

SA

slow-blow

(Tims

Lag)

High-Breaking-Capacity,

2

fuses

(UTTLEFUSE

215005)

tnu

Dm

ad.

Cut

Spt

COr

■ni

Fie

Rg.21

BX60

CONTROLS

Antlyisr

insertion

slot

Iwhtouh

safacur

knob

Potariwr

tfldor

insertion

slot

Raid

trie

dtaphreofn

knob

Aperture

int

■uaphnom

knob

tnterpupitlery

distance

adjustment

tctlt

Diopter

adjustment

ring

Aperture

m»

duphraem

ccntsrino

knob

Fwld

ifis

csnttring

knob

Tnn«mltudA*fl«ciad

Saht

(elector

witeh

Mjcfoacop#

mountiiig

bolt

holn

filter

lever

(LBD)

Filter

lover

INWI

Teneion

ediuetment

knob

O

The

above

illustration

shows

the

microscope

with

the

U-RIBC

mirror

cube

housing

for

the

brightfiQkVdarfcfiold

vertical

illuminator.

In

the

case

ol

the

IHJRBC

cube

housing

lor

the

universal

vertical

illuminator,

the

cube

selector

knob

is

replaced

by

a

turret.

[I

Thaixfletononly

Be

used

for

both

modal*

U-00

and

U-MOOB.

11

I

BrightfleloVDarfcfield

Vertical

Illuminator

Attachment

(U-RLBC/U-RLBU

Collector

Lens

Unit

(U-RLBU

O

Refer

to

section

6-5.

Reflacted

Light

Fluorescence

Observation,

for

operation

details.

|

Universal

Vertical

Illuminator

Attachment

(U-RLBC/U-RLBL)

BX60

Hoto

for

imchflMfit

of

the

»p»rtur«

Ut»

dliprtrtflro

fcnob

Hoi*

for

ttuchment

of

the

tpanura

Hols

for

m#thimiu

of

\lh«

fitid

lrl»

(Utphrwm

Imeb

Hole

for

•ttKhrmnt

of

trw

fWd

Irii

^diaphnflm

etnmtng

knob

Collector

lent

unit

(U-URBU

8

12

Biological

Microscopy

o

The

Jcflcwng

section

outfines

the

operations)

procures

v^

me

microscope

o

used

fw rwmid

(transmitted

fight

brightfieW

observation).

^^

*

UlS

objectives

tor

biological

microscopy

should

be

used.

Using

the

universal

cube

housing,

rotate

the

cube

turret

to

en

empty

position.

Using

the mirror

cube

housing,

slide

tho

cube

selector

cube

to

the

DF

position.

1.

Press

the

transrruttedAellected

light

selector

switch

to

select

the

transmitted

light

mode.

Turn

ON

the

main

switch

and

adjust

the

tight

intensity

with

the

light

intensity

lever.

(At

this

point

leave the

tight

intensity

preset

button

OFF)

(Page

17)

2.

Disengage

aU

filters

from

the

light

path.

(Pages

17.

181

a.

Accesssory

fitter

cassette

b.

Fitters

built

into

the

base

3.

Turn

the

revolving

noseptece

to

engage

the

10X

objective.

Make

sure

the

revolving

rtosepiece

dicks

into

position.

4.

Place

a

specimen

on

the

stage.

(Page

22)

(Using

a

Trinocular

Observation

Tubel

6.

Push

the

observation

tube's

tight

path

selector

knob

to "binocular

eyepiece

100%'

(the

pushed-in

position).

(Page

26)

&

Looking through

the

nght

eyepiece

with

your

tight

eye.

turn

the coarse

adjustment

knob

to

bring

the

specimen

into

focus.

After

obtaining

approximate

focus,

use

the

fine

adjustment

knob

to

make

final

adjustments.

(Page

25)

13

j.

ait

BX60

7.

Looking

through

the

left

eyepiece with

your

left

eye.

turn

ttie

diopter

adjustment

ring

to

focus

on

the

specimen.

(Page

25)

8

Adjust

the

werpuftflary

distance

of

the

eyepieces.

IPage

25)

9.

Adjust

condenser

centering

and

focusing.

(Page

27)

I

10.

Engage

the

objective

to

be used

and

readjust

the

tight

intensity

to

the

desired

level

for

observation,

then

readjust

the

focus.

11.

Engage

your choice

of

fitters

into

the

fight

path.

(Pages

17, 18, 19)

a.

Accessory

filter

cassette

b.

Filters

built

into

the

base

12.

Adjust

the

field

iris

diaphragm.

(Page

27)

13.

Adiusi

the

aperture

ens

diaphragm, (Page

28)

14

Metallurgical

Microscopy

The

following

section

outlines

the

operational

procedures

when

the

microscope

o

used

to

normal

metallurgical

rrucroscopy

(reflected

light

brigritfteldAJarkfield

observation)

with

the

brightfield/darkfiald

vertical

illuminator.

In

the

case

of

the

universal

vertical

illuminator.

the

cube

selector

knob

is

replaced

by

a

turret.

l.

Select

the

cube

according

to

the

observation

purpose.

Set

the

cube

selector

knob

©

to

BF.

I

2

m

15

2.

Disengage

the

filter

slider

©

(left

side of

arm),

analyzer

i

slider

0.

and

NO

filter

from

the

light

path.

(Page

33)

3.

Press

the

transmitted/reflected

light

selector

switch

to

select

the

reflected

light

mode.

Turn

ON

the

main

switch

and

adjust

the

light

intensity

with

the

light

intensity

lever.

(At

this

point

leave

the

light

intensity

preset button

OFF.)

(Page

171

4.

Place a

specimen

on

the

stage.

(Page

22)

5.

Turn

the

revolving

nosepiece

to

engage

the

10X

objective.

Bring the

specie'

into

focus.

6.

Adiusi

the

mterpupillary

distance

of

the

eyepieces.

(Page

2SI

BX60

7.

Looking

through

the

right

eyepcece

with

your

light

eye.

turn

the

coarse adjustment

knob

to

bring

the

specimen

into

focus.

After

obtaining

approximate

focus,

use

the

fine

adjustment

knob

to

make

final

focus

adjustments.

(Page

25)

B.

Looking

through

the

left

eyepiece

with

your

left

eye,

turn

the

diopter

adjustment

ring

to

focus

on

the

specimen.

(Page

25)

9.

Confirm

that

the

illumination

is

adequate

for

the

observation

purpose.

IPage

32)

10.

Engage

the

required

filter.

11.

Engage

the

objective

to

be

used

for

observation,

then

readjust

the

focus

and

adjust

the

light

intensity

to

a

suitable

level

for

observation.

12.

In

case

of

reflected

light

brightfteU

observation,

select

the

best

field

and

aperture

iris

diaphragm

settings

in

accordance

with

the

objective

and

specimen.

(Pages

20.

21)

1

16

Olympus BX60 User manual

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