Oxford Optronix HypoxyLab User manual

USER MANUAL

HypoxyLab User Manual
Revision 19 | March 2022
1
Product Docu entation
and User Manual
Revision 19
(Applicable to ‘Gen 2’ syste s; s/n HL9068 and later)
HypoxyLab™ is designed and manufactured by:
Oxford Optronix Ltd.
19 & 21 East Central
127 Oly pic Avenue
Milton Park
Oxford OX14 4SA
United Kingdo
t: [phone]
f: [phone]
Pri ary website: www.oxford-optronix.co
Sales e ail: [email protected]
Support website: www.oxford-optronix.co /support/hypoxylab-support
Support e ail: [email protected]
HypoxyLab™ is a registered trade ark of Oxford Optronix Ltd.
© Oxford Optronix Ltd. 2022. All rights reserved.

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Thank you!
Thank you for purchasing our popular HypoxyLab™ hypoxia workstation; we genuinely
value your co it ent.
At Oxford Optronix Ltd. we pour our heart and soul into developing and anufacturing
laboratory products to the highest standards that will eet or, ideally, exceed our
custo ers’ expectations.
For those rare but inevitable occasions where things go wrong, please note that your
HypoxyLab is covered by our 5-year anufacturer’s warranty*, during which ti e the costs
of any repair, including shipping charges where required, will be borne entirely by
ourselves. We will always endeavour to ini ize disruption and inconvenience through
any warranty clai .
We view our 5-year warranty as a state ent of our confidence in the quality of the
HypoxyLab. However, as for all laboratory equip ent of this type, regular preventative
aintenance is essential to ensuring opti al perfor ance, reliability, and longevity. Our 5-
year warranty is therefore contingent upon annual on-site preventative aintenance.
We wish you every success with forthco ing studies and stand ready to support your use
of the HypoxyLab in any way we can.
Please feel free to reach out to us via our support ailbox:
[email protected]
Sincerely,
Michael Rau, PhD
Sales Director
*Provided that paid-for on-site preventative aintenance is carried out annually

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Taking care of e
• Please keep e clean inside and out (you
can safely spray e down with 70%
ethanol)
• Please don’t leave e in ‘stand-by’ for
long periods (it hurts y gas sensors)
• Please e pty y water reservoir after
use (so it doesn’t go stagnant)
• Please don’t leave e all wet and da p after use (I like to
air-dry)
• Please clean and disinfect y water reservoir regularly
(there are instructions on our support site)
• Please release the catches on y enclosure cover when I’
not in use (it avoids defor ation of the base seal)
• Please supply e with sterile deionized or distilled water (it
avoids the build-up of scale)
• Please calibrate y CO
2
sensor and replace y HEPA filter at
least once per onth
• Please calibrate y oxygen sensor at least once every 6
onths
• Please fit y ther al jacket when I’ in use (it keeps e
war and reduces condensation)

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Table of Contents
1. S AF TY I NFORMATION 6
1.1 Sy bols used on HypoxyLab rear connector panel ............................................ 6
1.2 Su ary of Warnings ......................................................................................... 7
1.3 Su ary of Cautions .......................................................................................... 8
2. I NTRODUCTION 10
2.1 Opti ising Conditions for Cell-Based Research ................................................ 10
2.2 Key Benefits of the HypoxyLab .......................................................................... 11
3. I NSTALLATION R QUIR M NTS 13
3.1 Gas Supply Require ents ................................................................................. 13
3.2 Electrical Supply Require ents ......................................................................... 14
3.3 Physical and Location Require ents................................................................. 15
4. I NSTALLING AND U SING H YPOXY L AB 16
4.1 Support Videos .................................................................................................. 16
4.2 List of Standard Co ponents ............................................................................ 16
4.3 Unpacking and Description ............................................................................... 16
4.4 Connecting the Electrical Supply ....................................................................... 18
4.5 Connecting the Gas Supplies ............................................................................. 19
4.6 Water Supply / Wastewater .............................................................................. 20
4.7 Fitting of Shelf Units .......................................................................................... 21
4.8 Using the Ar port Sleeves ................................................................................ 22
4.9 Fitting the Ar port Plugs .................................................................................. 22
4.10 Using the Easy-Entry Hatch ............................................................................... 23
4.11 Fitting the Ther al Jacket ................................................................................. 24
4.12 Fitting Insulated Ar port Covers ...................................................................... 24
4.13 Re oving the Enclosure Cover ......................................................................... 25
4.14 Changing the Ar port Sleeves .......................................................................... 25
4.15 The Touchscreen User Interface ....................................................................... 26
4.16 Calibrating the CO
2
Sensor ................................................................................ 27
4.17 Calibrating the Oxygen Sensor .......................................................................... 28
4.18 Touchscreen Disable Function .......................................................................... 29
4.19 Manual Fan Speed Control (Reducing Evaporation) ......................................... 29
4.20 Saving Recorded Data to USB Flash Me ory .................................................... 30
4.21 Applying a Software Upgrade............................................................................ 30
4.22 The Vacuu Thro-Port ...................................................................................... 31
4.23 Using OxyLite™ for in situ Oxygen Measure ents............................................ 31
4.24 Setting up the optional CytoSMART™ add-on ................................................... 32
5. C AR AND M AINT NAC 34
5.1 Cleaning and Sanitising ..................................................................................... 34

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5.2 Routine Preventative Maintenance .................................................................. 34
5.3 Returning HypoxyLab for Repair, Service, or Upgrade ...................................... 35
5.4 Storage Reco endations for HypoxyLab ....................................................... 36
6. O BTAINING T CHNICAL A SSISTANC 37
6.1 Technical Support.............................................................................................. 37
6.2 Support Site and Support Videos ...................................................................... 37
7. T CHNICAL S P CIFICATIONS 38
8. W ARRANTY 40
A PP NDIX 1
Converting between % oxygen and Hg 41
A PP NDIX 2
Evaporation and reco endations for relative hu idity (RH) settings 42

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1. SAF TY INFORMATION
This section contains i portant safety infor ation related to the general use of the
HypoxyLab. Other i portant safety infor ation appears throughout this anual in the
for of warnings and cautions.
1.1 Symbols used on HypoxyLab rear connector panel
SN
Serial nu ber
R F
Catalogue nu ber
(product code)
Date of anufacture
Equip ent should NOT be
disposed of in the nor al
waste strea
Warning – see user
anual
Read user anual
N2
Nitrogen gas inlet
CO
2
Carbon dioxide gas inlet
Air
Air gas inlet USB interface
Serial interface
Product co plies with EU
safety standards

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1.2 Summary of Warnings
A Warning indicates a condition leading to the possibility of serious injury or even death.
WARNING
Like any incubator, ALWAYS use HypoxyLab in a well-ventilated roo
or laboratory.
WARNING
As for all tissue culture roo s or laboratories containing incubators,
we strongly reco end the use of wall/ceiling ounted oxygen
depletion warning sensors.
WARNING
While due care is taken to ensure gas hose connectors and regulator
fittings supplied by Oxford Optronix are leak-free it is ESSENTIAL to
check for gas leaks, both at the gas source end and at the HypoxyLab
end of gas lines once they are connected and pressurized.
WARNING
DO NOT operate the HypoxyLab with the enclosure cover re oved. To
do so ay cause uncontrolled CO2 venting.
WARNING
DO NOT atte pt to re ove the underside base plate. There is a risk of
electrical shock.
WARNING
DO NOT supply gases to the HypoxyLab other than the ones specified.
WARNING
DO NOT exceed the axi u pressure ratings of the inlet gases
supplying the HypoxyLab.
WARNING
DO NOT exceed the axi u voltage ratings of the inlet power
supplying the HypoxyLab.
WARNING
ALWAYS replace fuses with the sa e type and rating. Failure to do so
ay present a fire hazard.
WARNING
The rear surface of the turret structure (see Fig 4.1) ay beco e HOT
TO THE TOUCH. Allow at least 5 inutes fro powering down the
HypoxyLab before touching the rear of the turret (e.g. to change the
HEPA filter).
WARNING
Failure to pack the HypoxyLab adequately for repair or service ay
cause costly da age during transit.
WARNING
No user-serviceable parts inside. HypoxyLab MUST be repaired or
serviced by Oxford Optronix trained or approved personnel only.

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1.3 Summary of Cautions
A Caution indicates a condition that ay lead to a alfunction and/or per anent da age
of the HypoxyLab.
CAUTION
BEFORE UNPACKING carefully inspect the outer shipping carton for
signs of da age. Please take photographs as evidence and
IMMEDIATELY report any packaging da age to Oxford Optronix Ltd or
your authorised distributor.
CAUTION
HEAVY ITEM. The HypoxyLab weighs approxi ately 30 Kg (70 lbs).
TWO PERSONS are required to safely lift and ove the HypoxyLab.
CAUTION
FRAGILE TURRET. Never handle the turret structure to lift or ove the
HypoxyLab. Move the HypoxyLab by gripping the underside of the
base only.
CAUTION
Gas inlet lines and their integrity are the responsibility of the user and
should be constructed, tested and aintained in accordance with
local Code of Practice standards.
CAUTION
It is the responsibility of the user to fit ulti-stage regulators to all gas
cylinders supplying gasses to the HypoxyLab.
CAUTION
It is the responsibility of the user to fit the correct ulti-stage
regulator according to the type of gas used.
CAUTION
DO NOT site the HypoxyLab near draughts caused by doors, windows
or air conditioning/ventilation ducts.
CAUTION
DO NOT site the HypoxyLab on paper towels, tablecloths or any other
aterials that ight obstruct the air vents on the underside of the
HypoxyLab.
CAUTION
DO NOT operate the HypoxyLab outside the specified operating
te perature range of 15 – 30
º
C.
CAUTION
ONLY use DIONIZED / DISTILLED WATER to supply the HypoxyLab.
Oxford Optronix shall not be held responsible for da age caused by
the build-up of scale in the reservoir.
CAUTION
DO NOT leave the HypoxyLab in Stand-by ode for extended periods.
Sensor accuracy and longevity ay be adversely affected.

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CAUTION
NEVER use a onia-based cleaning agents, Methanol, Toluene or
Acetone to clean HypoxyLab. To do so will degrade and da age the
aterials used within.
CAUTION
It is ESSENTIAL to e pty the reservoir during periods when HypoxyLab
is not in use. Oxford Optronix will not be held responsible for da age
caused by the build-up of icrobial conta ination in the reservoir due
to stagnant water.
CAUTION
Regular (bi- onthly) deconta ination of the reservoir is ESSENTIAL to
prevent the build-up of icrobial conta ination over ti e. Refer to
Section 5.2.
CAUTION
It is reco ended to aintain the ar port sleeves INSIDE the
HypoxyLab or within the insulated ar port covers during extended
(e.g. overnight) incubation periods. Allowing the ar port sleeves to
‘dangle’ outside the HypoxyLab can result in the accu ulation of
water condensation.
CAUTION
DO NOT use sharp objects to operate the touch screen display.
CAUTION
DO NOT spray, pour or spill any liquid on the touch screen display.
CAUTION
DO NOT atte pt to operate the HypoxyLab in the vicinity of
equip ent that e its ionising radiation or produces a strong agnetic
field as the perfor ance of the device ay be adversely affected.
CAUTION
Gases supplied to the HypoxyLab ust be equal to or better than
99.995% pure ‘zero’ grade (N4.5) nitrogen.
CAUTION
Use ONLY the HEPA filters supplied by Oxford Optronix.

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2. INTRODUCTION
2.1 Optimising Conditions for Cell-Based Research
HypoxyLab™ offers researchers a new toolkit to accurately reproduce real-life
physiological conditions for a wide variety of cell-based research fields, including cancer
biology, radiation cell biology, cardiovascular research, apoptosis, neurology, ste cell
research, ultidisciplinary drug develop ent, and proteo ics.
Interest in the role of hypoxia in cell develop ent has gained o entu as researchers
learn ore about the biology of physiological oxygen concentration and how it affects cells
at the olecular level. While in the past the focus in the laboratory was on aintaining
cells alive and genetically stable while avoiding conta ination, in recent decades
researchers have de onstrated that cells react in different ways, both etabolically and
orphologically, depending on the environ ental factors that aintain and interact with
the .
Today’s life scientists require the ability to reproduce in vivo environ ents in which cells
can be cultured in their natural state, at oxygen concentrations in the range of 0.5 - 10%.
To date all hypoxia workstations present the user with a ethod for controlling oxygen as
a percentage concentration (% O
2
) only. However, this approach does not take account of
baro etric pressure and therefore fails to deliver co pensation for altitude of the
research location or prevailing cli atic conditions.
HypoxyLab is a second-generation hypoxia workstation that uses the absolute partial
pressure of oxygen, expressed in units of Hg or kPa, to deliver authentic hypoxia
replication with the highest possible accuracy. Since the partial pressure of oxygen is what
cells actually “see” when exposed to oxygen, this scientifically superior approach enhances
hypoxia accuracy, particularly when culturing cells at extre ely low oxygen set-points.
Regardless therefore, of whether a research laboratory is located in A sterda or in
Denver, hypoxia conditions generated within the HypoxyLab in one location can be reliably
co pared with those generated in another.
HypoxyLab delivers precise control of the partial pressure of oxygen and carbon dioxide
content, alongside cha ber te perature and hu idity, using opti ised PID algorith s
alongside advanced digital gas flow controllers, low aintenance or aintenance-free
sensors, and built-in nebulizer-based hu idifier technology. Using its touchscreen display,
researchers also have the ability to set HypoxyLab to auto atically cycle through up to
eight fully progra able oxygen profiles.
HypoxyLab is also engineered to support our “gold standard” OxyLite™ dissolved oxygen
onitors. Positioning an OxyLite sensor within a cell culture provides a direct easure of
the partial pressure of oxygen that cells are actually exposed to and can optionally be
recorded alongside the values of cha ber pO
2
, CO
2
, te perature, and hu idity.
For research tea s undertaking studies at the cellular and olecular level, HypoxyLab
provides a way to aintain cells at true physiological oxygen, an essential prerequisite to
refining the processes they are using to probe gene expression changes, signal

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transduction pathways, enzy e activities, and the olecular echanis s that underlie
these pheno ena.
2.2 Key Benefits of the HypoxyLab
True physiological oxygen (Physoxia)
As every scientist knows, it’s the partial pressure of oxygen that reflects the oxygen that
cells actually ‘see’. The partial pressure of oxygen varies not only with oxygen
concentration in the at osphere, but also with altitude and prevailing weather conditions.
We believe it is si ply i plausible to express hypoxia in ter s of a (baro etrically
unco pensated) percent oxygen concentration alone. HypoxyLab is the world’s first and
only hypoxia cha ber that directly regulates its environ ent using the partial pressure of
oxygen, expressed in units of Hg or kPa, and is thereby insensitive to changing cli atic
conditions or the altitude of the laboratory. This scientifically rigorous approach enhances
HypoxyLab's accuracy and provides researchers with scientific certainty and the ability to
replicate ‘real-life’ hypoxia in vitro at the highest level of accuracy achievable.
Rapid, efficient, accurate
HypoxyLab creates a fully hu idified, stable hypoxia environ ent less than 20 inutes
fro switch-on and can deliver stable, near-anoxia conditions in under an hour, all while
consu ing surprisingly little nitrogen gas. Environ ental conditions are delivered using
digital gas flow controllers and state-of-the-art sensors for unrivalled perfor ance with an
oxygen accuracy of up to +/- 1 Hg.
n situ dissolved oxygen in media and cultures
HypoxyLab supports the easure ent of dissolved oxygen directly fro edia or cell
cultures using any of our OxyLite™ oxygen onitors and a dedicated, sealable side-gland
via which an OxyLite oxygen sensor can be fed into the cha ber. Considered the ‘gold
standard’ for biological applications, the OxyLite sensor is a tiny, highly accurate oxygen
sensor that does not itself consu e oxygen and that can be positioned anywhere within
edia or cell cultures to obtain continuous dissolved oxygen easure ents. This provides
a easure of oxygen availability in the icroenviron ent where cells are actually growing.
Oxygen readings (in Hg) can be read fro the OxyLite display or can even be displayed
and recorded by the HypoxyLab itself.
Live cell imaging
We have tea ed with CytoSMART™ to offer the Lux2 digital icroscope syste , an
extre ely co pact digital icroscope solution that can be deployed within the HypoxyLab
and is built to withstand long-ter exposure to tissue culture environ ents. The syste is
supplied with software for installation on a PC kept outside the HypoxyLab and on which
live cell culture i ages are displayed. The CytoSMART™ Lux2 supports ti e-lapse
recording and cloud-based data access.

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Contamination control
HypoxyLab includes a built-in UV source within the water reservoir and a user replaceable
HEPA filter that continuously scrubs the cha ber at osphere, ensuring that cultures and
edia are protected fro conta ination.
Touchscreen display
Cha ber oxygen, CO2, hu idity, and te perature are all set and controlled fro the
intuitively designed, integrated touchscreen, which si ultaneously displays the current
real-ti e levels of these para eters in both digital and trace for ats.
Automated oxygen profiles
A fully custo izable oxygen profile progra is available fro the touch screen, with which
the HypoxyLab can be set to auto atically subject cells to up to 8 sequentially defined
oxygen concentrations, including an option to keep looping the progra .
Data logging
The 4 key environ ental para eters are continuously recorded onto internal flash
e ory and can be exported to a USB flash drive via a port at the rear of the HypoxyLab
at any ti e. Data files can be analysed and played back using the free LabChart® Reader by
ADInstru ents.
asy- ntry system
HypoxyLab’s Easy-Entry hatch syste affords quick and convenient transfer of cells,
culture-ware and edia into the cha ber without the need for co plicated air locks and
isolation hatches. While ite s are passed through the hatch, HypoxyLab applies the
precise gas co position necessary to aintain steady-state conditions. This i ediate
response eli inates fluctuations in the pO2 of cell edia during operation of the Easy-
Entry syste . An internal ‘letterbox’ style flap acts as an additional barrier against gas
displace ent when the hatch is opened.
Lightweight cover / rgonomic design
HypoxyLab’s lightweight and durable cover can be easily re oved. This not only allows for
easy loading of edia and consu ables but akes routine cleaning and disinfecting of the
workstation a practical reality. Its ergono ic design ensures natural, relaxed operation,
with an angled vision panel co bined with adjustable LED illu ination that provide
excellent visibility.
Cost of ownership advantage
HypoxyLab’s co pact and considered design ensures rapid equilibration to set points
co bined with frugal gas consu ption. Service-free sensors and a user changeable HEPA
filter si plify routine aintenance and ini ize the long-ter cost of owning a
HypoxyLab.

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3. INSTALLATION RQUIR M NTS
3.1 Gas Supply Requirements
WARNING
DO NOT atte pt to supply gasses to the HypoxyLab other than the ones
specified. To do so ay cause death or injury.
WARNING
DO NOT exceed the axi u pressure ratings of the inlet gasses
supplying the HypoxyLab. To do so ay cause death or injury.
CAUTION
Gas inlet lines and their integrity are the responsibility of the user and
should be constructed, tested and aintained in accordance with local
Code of Practice standards.
CAUTION
It is the responsibility of the user to fit ulti-stage regulators to all gas
cylinders supplying gasses to the HypoxyLab.
CAUTION
It is the responsibility of the user to fit the correct ulti-stage regulator
according to the type of gas used.
CAUTION
Gases supplied to the HypoxyLab ust be equal to or better than
99.995% pure ‘zero’ grade (N4.5) nitrogen.
Only laboratory-grade (ideally 99.995% pure ‘zero’ grade (N4.5) purity) gases ust be used
with the HypoxyLab. Where cylinders are used, it is vital that high quality, ulti-stage
regulators are fitted to each gas cylinder and that each regulator is correctly specified for
the particular type of gas used. Each regulator requires a 6 (¼ inch) NPT fitting to
allow the hoses supplied with the HypoxyLab to be connected (see Fig. 3.1 below).
It is the responsibility of the user to ensure that supplied gases are free of particulate
contamination. Unfiltered gases entering the HypoxyLab may damage the delicate
electronic flow controllers within. Oxford Optronix shall not be held responsible for
damage caused by particulates entering the HypoxyLab. We recommend the use of 30
µm or finer upstream in-line filters for any building-wide ‘plumbed-in’ gases.
High quality, ulti-stage regulators pre-fitted with NPT hose fittings can be purchased
fro Oxford Optronix Ltd.

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Figure 3.1 Multi-stage regulator and barbed NPT fitting
The HypoxyLab requires CO
2
, synthetic air and nitrogen gases to be supplied at pressures
su arized below:
Gas type
Reco ended purity
‘N4.5’ or better
Outlet Regulator No inal outlet pressure
CO
2
6 (¼“) NPT Two Stage 1 – 4 bar (15 - 60 psi)
Synthetic air 6 (¼“) NPT Two Stage 1 – 4 bar (15 - 60 psi)
Nitrogen 6 (¼“) NPT Two Stage 3 – 5 bar (45 - 75 psi)
3.2 Electrical Supply Requirements
WARNING
DO NOT exceed the axi u voltage ratings of the inlet power
supplying the HypoxyLab.
WARNING
ALWAYS replace fuses with the sa e type and rating. Failure to do so
ay present a fire hazard.
The input voltage require ents for the HypoxyLab are as follows:
Input Voltage: 110 – 240 VAC 50/60 Hz Single phase
Max Operating Current: 5A
Max Operating Power: 550W
Fuses: 2 x 5A 20 AS
A dedicated, country-specific IEC ains inlet cable is supplied with your HypoxyLab
.
Gauges (internal bottle pressure +
controllable supply pressure)
Outlet connection. Requires 6 mm
(¼”) NPT barbed’ hose-end fitting
(see inset below)
Connection to gas bottle (format and
thread standards are often country-
specific)
Supply pressure control tap
6 mm (¼”) NPT barbed’
hose-end fitting

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3.3 Physical and Location Requirements
CAUTION
DO NOT site the HypoxyLab near draughts caused by doors, windows, or
air conditioning/ventilation ducts.
CAUTION
DO NOT site the HypoxyLab on paper towels or tablecloths or any other
aterials that ight obstruct the vents on the underside of the
HypoxyLab.
CAUTION
DO NOT operate the HypoxyLab outside the operating te perature
range of 15 – 30
º
C
HypoxyLab ust be operated in a clean laboratory environ ent free fro corrosive gases,
fla able gases, and dust.
Instructions for asse bling the (optional) electrically height-adjustable obile worktable
(product code ‘WORKTABLE’) are provided separately.
If HypoxyLab is to be bench ounted, the bench ust be stable, flat, level and of sufficient
size and strength to fully support the HypoxyLab.

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4. INSTALLING AND USING HYPOXYLAB
4.1 Support Videos
A collection of video tutorials covering any of the sections below is aintained on the
HypoxyLab support website:
http://www.oxford-optronix.co /support/supp_hypoxylab.ht
4.2 List of Standard Components
• 1 pair of ar port sleeves, pre-fitted
• 1 base seal, pre-fitted
• 1 HEPA filter, pre-fitted
• 2 spare HEPA filters
• 2 shelf units (co patible with 50 l and 15 l Falcon tubes)
• 1 stainless steel elevated floor unit
• 1 neoprene ther al jacket
• 1 Oxford Optronix branded USB flash drive
• 3 gas inlet hoses, 3.5 (1 each for Nitrogen, CO
2
, and synthetic air)
• 1 pair of wrist seal plugs
• 1 pair of spare latex wrist seals
• 1 pair of water fill/drain hoses
• 1 pair of insulated ar port covers
• 1 100 l starter bottle of Klercide A ine concentrate (water reservoir sterilizer)
• 1 printed user anual
• 1 country-specific IEC ains power cable
4.3 Unpacking and Description
Before unpacking the HypoxyLab, ensure the physical, electrical and gas supply installation
require ents listed in the previous section have been followed.
When unpacking the HypoxyLab, inspect the packaging thoroughly for any signs of da age
that ay have occurred during transit. Report any da age i ediately to Oxford
Optronix Ltd. or your local authorised distributor.
CAUTION
HEAVY ITEM. The HypoxyLab weighs approxi ately 30 Kg (70 lbs). TWO
PERSONS are required to safely lift and ove the HypoxyLab.

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CAUTION
FRAGILE TURRET. Never handle the turret to lift or ove the HypoxyLab.
Move the HypoxyLab by gripping the underside of the base only.
Figure 4.1 Frontal view of the Hypo yLab
Turret structure
LED lighting
Enclosure
cover
Enclosure
cover latch
Over-pressure
bleed valve
Easy-Entry
hatch
Touchscreen
display
Armport (including
sleeve & plug)
Shelf unit
OxyLite sensor
gland

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Figure 4.2 Hypo yLab rear connector panel
To unpack the HypoxyLab, re ove the retaining straps that surround the shipping carton
and that run under the wooden pallet, re ove all protective cellophane wrapping, open
the top of the carton and re ove the foa insert protecting the top of the HypoxyLab.
Re ove any packing tape connecting the base carton to the upper carton then carefully
lift the upper carton ‘up and over’ to reveal the HypoxyLab secured within its base foa
insert.
The HypoxyLab weighs approxi ately 30 kg (70 lbs). It should be lifted out of the base
foa insert by TWO PERSONS by locating the gripping points in the foa insert either side
of the HypoxyLab.
Carefully position the HypoxyLab on a worktable or previously prepared laboratory bench.
4.4 Connecting the Electrical Supply
Locate the IEC ains power cable (supplied in the accessories box) and plug it into the
ains inlet connector on the HypoxyLab back panel (Fig. 4.2). Switch on the HypoxyLab,
when ready to do so, by operating the rocker switch. The HypoxyLab will boot up to
‘Stand-by’ ode.
CAUTION
DO NOT leave the HypoxyLab in Stand-by ode for extended periods.
Sensor accuracy ay be adversely affected.
Gas hose
connectors
IEC mains inlet
(containing fuses)
and on/off switch
USB flash media
connector
Serial connector for
OxyLite monitor
Drain hose
connector
Inlet hose connector
(strictly de-ionized
water)
Device serial
number and date
of manufacture

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4.5 Connecting the Gas Supplies
WARNING
As for all tissue culture roo s or laboratories containing incubators, we
strongly reco end the use of wall/ceiling ounted oxygen depletion
warning sensors.
WARNING
While due care is taken to ensure gas hose connectors and regulator
fittings supplied by Oxford Optronix are leak-free it is ESSENTIAL to
check for gas leaks, both at the gas source end and at the HypoxyLab
end of gas lines once they are connected and pressurized.
The inco ing gas supplies are the sole responsibility of the user. It is essential that the
user has read and fully understood Section 3.1 ‘Gas Supply Require ents’ before
connecting the gas supply hoses.
Three ‘Quick Connect’ style gas connection hoses, pre-fitted with high quality SwageLok®
fittings are supplied with your HypoxyLab. These hoses are for supplying CO
2
, air and
nitrogen and are labelled accordingly. The connector asse blies are supplied ‘clean’. The
hoses should be attached to the outlet stage of each regulated cylinder according to the
table below.
Take a o ent to double-check that each labelled hose is securely connected to the
correct gas (cylinder). Also, double-check that the regulator output pressures are correctly
set according to the table below.
Locate and identify the individual ale inlet gas connectors on the back of the HypoxyLab.
Push the brass SwageLok® fitting end of each labelled gas hose fir ly onto the
corresponding gas inlet connector, gripping the fitting by the body rather than by the front
flare (as shown above). An audible ‘click’ confir s that the SwageLok® fitting is secured