PASCO PS-3514 User manual

Instruction Sheet
012-15716A
Flat pH Probe
PS-3514
Introduction
This electrode is a hand crafted, precision analytical
device. Carefully follow the directions in this
instruction sheet to obtain the best performance and
electrode life.
Required Equipment and Solutions
• PASCO Wireless pH Sensor (PS-3204)
or
• PASPORT pH Sensor (PS-2107A) with a
PASCO interface (see www.pasco.com for
information)
or
• pH meter (a temperature compensator is
recommended for increased pH measurement
accuracy)
• PASCO data collection software (see
www.pasco.com)
• pH Buffer Solution 4.01*
• pH Buffer Solution 7.00*
• Wash bottle filled with distilled or de-ionized
water
• Laboratory magnetic stirrer and magnetic stir
bar
• Lab wipes
• Clean beakers**
(*SC-2321 pH Buffer Capsule Kit)
(**SE-7287 100 ml or SE-7288 1000 ml Beakers)
Included Equipment Item
Flat pH Probe 1
Male-to-female BNC Cable 2
Storage Bottle 3
3
2
1

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Flat pH Probe Introduction
2012-15716A
Preparation of the Electrode for Initial Use
1. Remove the storage bottle from the electrode and
thoroughly rinse the electrode with distilled water.
Wipe carefully with a clean lab wipe.
2. During shipment, air bubbles may have migrated
into the electrode sensing bulb. Hold the electrode
up to the light and inspect the sensing bulb for air
bubbles. If air is seen, carefully shake the electrode
downward (like a thermometer) to dispel the air
bubble from the sensing bulb at the tip of the
electrode.
Software Help
See the SPARKvue Help or PASCO Capstone Help for
information about collecting, displaying, and analyzing
data.
• In SPARKvue, select the HELP button in any screen
including the Home Screen.
• In PASCO Capstone, select PASCO Capstone Help
from the Help menu, or press F1.
Standardizing the Electrode
1. Connect your pH electrode to the input connector on
the Wireless pH Sensor (or other pH measuring
device). Ensure that the electrode connection is
secure.
2. Start the PASCO data collection software if you are
using a PASCO sensor. Connect the sensor to your
computing device (e.g., computer or tablet).
3. Start recording data. Place the electrode into a
beaker containing pH 7.00 buffer and a stir bar. Stir
at a moderate speed.
4. When the reading is stable, set the calibration value
in the software to match the value of the pH 7.00
buffer at the temperature in the laboratory.
5. Remove the electrode from the buffer. Rinse with
distilled water and blot with a lab wipe.
6. Place the electrode in a beaker containing pH 4.01
buffer and a stir bar. Stir as before. When the
reading is stable, set the calibration value in the soft-
ware to match the value of the 4.01 buffer at the
temperature in the laboratory.
Reading a Sample with the Electrode
1. Rinse the electrode with distilled water and blot with
a lab wipe. Place the electrode in a beaker
containing the sample and a stir bar. The sam-ple
should be at the same temperature as the buffers
used to standard-ize the electrode. Stir as before.
Record the pH when the reading is sta-ble.
2. Remove the electrode from the sample, rinse the
electrode with distilled water over the "waste"
beaker. Blot the electrode dry with a lab wipe. The
electrode is now ready to read the pH of other
samples.
Storing the Electrode
Short Term
Between measurements, store the pH electrode in a
beaker containing pH 4.01 buffer.
Long Term
When storing for longer periods, store the pH electrode
in the storage bottle which came with the electrode.
Ensure that the foam in the storage bottle is totally
wetted with pH storage solution to keep a wet
environment around the pH bulb and junction. Maintain
the wet environment in the storage bottle boot during
storage.
Electrode Cleaning
Do not use strong solvents (e.g. acetone, carbon
tetrachloride, etc.) to clean the pH electrode. Be sure to
recalibrate the electrode after cleaning.
1. If the electrode has become coated with oil or
grease, carefully wash the electrode under warm tap
water using dish-washing detergent. Rinse
thoroughly with fresh tap water followed by a rinse
with distilled water. Soak the electrode in pH
electrode storage solution for 30 minutes after this
cleaning procedure. Recalibrate the electrode
before use.
2. If the electrode has been exposed to protein or
similar materials, soak in acidic pepsin for 5
minutes. Rinse thoroughly with distilled water. Soak
in storage solution for 30 minutes prior to
recalibration.
3. If the previous cleaning procedures fail to restore
response, soak the electrode in 0.1 N HCI for 30
minutes. Rinse thoroughly with distilled water.
Recalibrate before use.

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PS-3514 Technical Support
3
012-15716A
4. If electrode response is not restored still, replace the
electrode.
Technical Support
For assistance with any PASCO product, contact
PASCO at:
Limited Warranty
For a description of the product warranty, see the PASCO catalog.
Copyright
This PASCO scientific instruction sheet is copyrighted with all rights
reserved. Permission is granted to non-profit educational institutions for
reproduction of any part of this manual, providing the reproductions are
used only in their laboratories and classrooms, and are not sold for profit.
Reproduction under any other circumstances, without the written consent
of PASCO scientific, is prohibited. Rev: 10/17
Trademarks
PASCO, PASCO scientific, PASCO Capstone, PASPORT and
SPARKvue are trademarks or registered trademarks of PASCO scientific,
in the United States and/or in other countries. All other brands, products,
or service names are or may be trademarks or service marks of, and are
used to identify, products or services of, their respective owners. For more
information visit www.pasco.com/legal.
Product End of Life Disposal Instructions:
This electronic product is subject to disposal and recycling regulations that
vary by country and region. It is your responsibility to recycle your
electronic equipment per your local environmental laws and regulations to
ensure that it will be recycled in a manner that protects human health and
the environment. To find out where you can drop off your waste equipment
for recycling, please contact your local waste recycle/disposal service, or
the place where you purchased the product.
The European Union WEEE (Waste Electronic and
Electrical Equipment) symbol (to the right) and on the
product or its packaging indicates that this product
must not be disposed of in a standard waste
container.
Address: PASCO scientific
10101 Foothills Blvd.
Roseville, CA 95747-7100
Phone: [phone] (worldwide)
800-772-8700 (U.S)
Email: [email protected]