Peak C-7100 Series User manual

C-7100 Series User Manual PEAK INSTRUMENTS INC.
C-7100 Series Spectrophotometer
Operating Manual and Maintenance Guide
PEAK INSTRUMENTS INC.
Version 1701

C-7100 Series User Manual PEAK INSTRUMENTS INC.
CONTENT
I. Spectrophotometer Overview ...................................................................................................................................1
1. Basic operating principle ...........................................................................................................................................1
2. Performance Indicators Definitions ..........................................................................................................................3
3. Application ................................................................................................................................................................4
4. Using conditions ........................................................................................................................................................4
II. Product features and installation
1. Features ....................................................................................................................................................................5
2. Performance indicators ............................................................................................................................................6
3. Main Functions .........................................................................................................................................................7
4. Structure Introduction ..............................................................................................................................................8
5. Instrument Installation ……………………………………………………………….………………………………………………………………………10
III. Key definitions and basic operation .........................................................................................................................11
1. Button panel schematic diagram ..............................................................................................................................11
2. Button function description ......................................................................................................................................11
3. Basic operation ..........................................................................................................................................................12
IV. Instrument self-test ..................................................................................................................................................12
1. Communication port inspection……………………………………………………..…………………………………………………………………….12
2. File system check .......................................................................................................................................................12
3. Filter positioning ........................................................................................................................................................12
4. Light positioning ........................................................................................................................................................12
5. Printer Check .............................................................................................................................................................13
6. Tungsten lamp examination ......................................................................................................................................13
7. Deuterium lamp examination ....................................................................................................................................13
8. Signal detector check .................................................................................................................................................13
9. Wavelength Calibration .............................................................................................................................................13
10. Dark current correction ...........................................................................................................................................13
11. System parameters check ........................................................................................................................................13
V. Photometric measurement .........................................................................................................................................14
1. Function description ...................................................................................................................................................14
2. Set the measurement mode .......................................................................................................................................14
3. Set wavelength ...........................................................................................................................................................15
4. Correction 100%T/0Abs ..............................................................................................................................................15
5. Measurement data .....................................................................................................................................................15
6. Delete data .................................................................................................................................................................16
7. Save the file ................................................................................................................................................................16
8. Open the file …………………………………………………………………………………………………………………………………………………………17
VI. Quantitative measurement ........................................................................................................................................17
1. Set parameter .............................................................................................................................................................18
2. Standard sample measurement ..................................................................................................................................18
3. Sample measurement ..................................................................................................................................................19
4. Open standard curve ....................................................................................................................................................19

C-7100 Series User Manual PEAK INSTRUMENTS INC.
VII. Time Scan (Kinetics)……………………………………………………………………………………………………………….………………………..…20
1. Function description ..............................................................................................................................................20
2. Time scanning parameters set ...............................................................................................................................20
3. Data test .................................................................................................................................................................21
4. Data list ..................................................................................................................................................................21
5. Slope calculation.....................................................................................................................................................22
VIII. Wavelength scanning ..........................................................................................................................................22
1. Function description ..............................................................................................................................................22
2. Set scanning parameters .......................................................................................................................................22
3. System baseline .....................................................................................................................................................23
4. Create a user baseline (corrected blank) ...............................................................................................................24
5. Begin testing ..........................................................................................................................................................25
6. Peaks and troughs .................................................................................................................................................25
7. Data list .................................................................................................................................................................26
IX. Multi-wavelength test .......................................................................................................................................26
1. Function description ...............................................................................................................................................26
2. Set parameter .........................................................................................................................................................27
3. Correction 100%T/0Abs ..........................................................................................................................................27
4. Data test .................................................................................................................................................................27
X. Protein / nucleic acid measurement....................................................................................................................28
1. Function description ..............................................................................................................................................28
2. Set parameter ........................................................................................................................................................29
3. Set Zero ..................................................................................................................................................................30
4. Test…………………………………………………….…………………………………………………………………………………………………….30
XI. System setting .......................................................................................................................................................30
1. Dark current measurements....................................................................................................................................31
2. Looking for deuterium lamp curve ..........................................................................................................................31
3. Time and date settings ............................................................................................................................................32
4. Light source management .......................................................................................................................................32
5. General ....................................................................................................................................................................33
6. File System ...............................................................................................................................................................33
7. USB storage device ..................................................................................................................................................34
8. Restore factory settings ...........................................................................................................................................34
9. System information .................................................................................................................................................35
XII. Instrument Care and Maintenance ........................................................................................................................35
1. Routine precautions ................................................................................................................................................35
2. Instrument Care and Maintenance .........................................................................................................................36
XIII. Instrument Troubleshooting .................................................................................................................................36
1. Power failure ...........................................................................................................................................................36
2. Self-test failure ........................................................................................................................................................37
3. Other failure ............................................................................................................................................................37
XIV. Light source replacement .....................................................................................................................................38
1. Tungsten lamp replacement ....................................................................................................................................38
2. Deuterium lamp replacement .................................................................................................................................39

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I. Spectrophotometer Overview
1. Basic operating principle
1) The nature of absorption: spectrophotometric analysis method is the use of substances to choose a different
wavelength of light absorption characteristics established. Typically using a prism or grating to obtain
monochromatic light that passes through the continuous monochromatic solution, the solution was measured and
the absorption of each wavelength, the absorption spectrum curve obtained.
Absorption spectrum selective absorption of light from the material, which is the material of macroscopic phenomena,
and the nature of the molecular absorption is the result of internal movement and light interaction. When molecules
absorb certain wavelengths of spectral energy or by some wavelengths of the spectrum is absorbed to form the
absorption spectra. The smaller the energy absorption, the wavelength of light corresponding to the absorption peak at a
longer wavelength. When the infrared absorbent is formed in the infrared absorption spectrum, if the energy absorption
is larger, the shorter the wavelength corresponding to the absorption peak at a shorter wavelength, when generating the
ultraviolet absorption spectra of absorption in the ultraviolet region.
2) Absorption Law - Lambert Beer law: when a parallel beam through the homogeneous solution, the absorbance of
monochromatic light is proportional to the product of the solution concentration and thickness.
Its digital expression: A=KCL=LogI/I0=-LogT
Premise absorption laws established numeric expression: ① the incident light is monochromatic ② the
absorption process without interaction of each substance, the absorbance of each substance has additivity ③ the
role of light and matter is limited to the absorption process, no fluorescent and photochemical scattering
phenomena ④ absorbent system is a continuous uniform distribution
3) Impact spectrophotometry reasons:
A. Non-absorption errors caused by radiation and matter
B. Fluorescence and photochemical reactions, in general, errors fluorescence spectrophotometry produced
negligible fluorescence efficiency is very small in most cases the color system, and the fluorescence emission is
isotropic, only a small portion along the transmitted light direction into the detector, the measurement of
absorbance is low, resulting in a negative deviation. Depends on the instrument to measure the impact on the
absorption of fluorescence great extent on the optical absorption cell and detector design.
C. Reflection and scattering, absorption law applies only to homogeneous medium absorption system, turbid solution so
that the measured increase in absorbance due to scattering, resulting in deviation from Beer's law
D. Non-ideal instrument error caused
E. Beer's law deviation polychromatic contrast, the majority of the photometer can only get close to monochromatic light
with a narrow lumen, in fact, there is still a polychromatic nature, can lead to deviations from Beer's law. Deviation

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depends on the two monochromatic molar absorptivity difference △ε, | △ε | is very small, can be approximated that
monochromatic, at low concentrations, curve remains linear, but larger concentrations, with concentration increases, AC
curve bend more serious, there is Beer's law applies only to dilute solutions
F. Stray light, stray light entering the detector means is unnecessary components to be tested at other wavelengths
outside the range of the wavelength spectral bandwidth. The main dispersive element from a prism or grating
spectrometer, a mirror, a lens surface scattering, dust and other inner walls of monochromator components and
diffuse reflection and other scars, the stray light can cause serious measurement error. The instrument is the
smallest wavelength of energy, usually at a maximum stray light (such as deuterium lamp 220nm, tungsten lamp
340nm)
G. Slit width, the slit width of the spectrum affects not only the purity, but also affect the absorbance. When
quantitative analysis in order to obtain sufficient measuring signal, the slit should be larger, in the qualitative
analysis of the use of a smaller slit when the entrance slit and the exit slit width equal to the width of the slit caused
minimum error
H. Wavelength scale ruler of error, the wavelength of the gauge that wavelength accuracy of the instrument, such
as a large error or correction, the spectral measurements produce errors that affect the accuracy of absorbance
measurements (in the absorption spectrum of the peak of the more significant )
I. Impact of non-parallel incident, one of the prerequisites ear than the law is the use of a parallel incident beam to
ensure that all beams through the same thickness of the absorbing medium, when a large deviation from
parallelism when the incident beam, obviously lead to deviations from Beer's law. If the instrument is in moderate-
intensity beam deviation from parallelism, absorbance measurement error is generally caused by less than 0.5%
J. Photometric scale error, photometric accuracy of the scale that is transmittance, which directly affects the
accuracy of the magnitude of the error photometric measurements.

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2. Performance Indicators Definitions
1) Optical system: usually refers to the formal structure of the optical system, at present, domestic and international
institutions often used as a photometer industry type and CT auto collimation two structures;
2) Wavelength range: means for wavelength photometer can difference between maximum and minimum values of
the test;
3) Wavelength accuracy means the actual wavelength difference between the real wavelength and the setting
wavelength. Each wavelength photometer to be in a lot of points to check wavelength accuracy; spectrophotometer
wavelength accuracy is an important technical indicators, it qualitative, quantitative and structural analysis of the
impact is enormous. Way to check wavelength accuracy of many spectral lines as a standard filter praseodymium,
neodymium, holmium oxide filter, holmium oxide wavelength standard solution, deuterium lamp or low pressure
mercury lamp emission and interference filters and so on.
4) Wavelength repeatability: Wavelength Repeatability is the ability of the instrument to return the original
wavelength. It reflects the wavelength drive mechanism and the stability of the whole instrument;
5) Spectral bandwidth (sensitivity, resolution): refers to a peak spectral bands when the slit on the detector
detected through a monochromator energy half-width, expressed in nm wavelength, from another perspective to
understand this concept will more user-friendly: first, the monochromator exit slit represents not just the physical
size or geometry, it also represents the optical sense, this is the spectral bandwidth, we know that the light from the
failure of a single monochromator wavelength, but at a narrow wavelength spectral band are arranged in the order,
the number of spectral wavelength band comprises, represented by the spectral bandwidth. Spectral bandwidth is a
direct response to the quality level of monochromatic light from the monochromator out. The index with the
instrument resolution and sensitivity are similar, but different, they react photometer performance quality from
different sides. Resolution refers to the size of the instrument to distinguish two adjacent wavelengths ability
Sensitivity is measured at low concentrations do, when the concentration changes by one unit to the detector signal
caused by the change amount, it is subject to a calibration curve (standard curve as the horizontal axis, the
absorbance on the vertical axis) and a precision instrument itself restrictions. Two measurement precision of the
method is the same, the greater the sensitivity calibration curve slope, while the slope equal, the higher the
sensitivity, the better precision. It is noted that, in order to obtain accurate test results, the natural bandwidth of the
spectral bandwidth of the instrument (Spectral Bandwidth referred SBW) and the analysis of samples (Natural
Bandwidth referred NBW) ratio should be less than 0.1, more than 99.5% can be obtained so that the measurement
accuracy.

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6) Stray light: wavelength of stray light is irradiated onto the non-selection signal generated by the detector. It is an
important source of photometric analysis of errors, stray light limit the accuracy of the General Assembly high
concentration solution analysis. Stray light represented by T%;
7) Photometric range: Refers to meet the photometric test range in various technical indicators, represented by A or
T;
8) Photometric accuracy: the true average value refers to the degree of compliance with a plurality of measured;
photometric accuracy check is usually through the use of repeated measurements of the neutral density filter
carefully film to a standard photometric detection. A neutral density filter for light in a wavelength range having
almost the same transmittance (or absorbance) of the filter, and the use of its wavelength insensitive characteristic
bandwidth changes, to check the accuracy of the optical instrument and repeatability.
9) Photometric Repeatability: refers several times under the same conditions as measured in parallel, each parallel
line with the degree of determination between the results;
10) Noise: the sum of the instrument detects the unwanted signals, which is the purpose of the relative signal.
Generally, the spectrophotometer has two sources of noise, one from the light source, the second is derived from
the internal electronic noise inherent in the instrument, such as: power supply, amplifier, AD conversion and the like.
To reduce noise, improve signal to noise ratio, there must be a good electrical design. Noise measurement
repeatability test under low concentrations, but also affect test accuracy. Noise by averaging several measurements
after partial elimination;
11) Drift: refers to the degree of deviation from the instrument over time the starting value. It depends on the
stability, light stability of the electrical device and the like. Single-beam instrument, the warm-up time has a great
influence on the length of the drift.
12) Baseline flatness: refers to the distribution of the full wavelength range of the instrument noise.
3. Application
Subjects available for physics, chemistry, medicine, biology, pharmacology, geology and other scientific research, is
widely used in chemical, pharmaceutical, biological and chemical, metallurgy, light industry, materials,
environmental protection, medicine and other industries and laboratory analysis of the industry's most one
important quality control instruments, is essential for routine laboratory instruments.
4. Using conditions
Equipment should be installed away from the hot or humid environment. The instrument should be used in 16-35 ℃,
45-80% humidity conditions. Please try to stay away from issuing magnetic, electric, high-frequency waves of
electrical devices, do not install the instrument in the air chlorine, hydrochloric acid gas, hydrogen sulfide gas, such
as sulfurous acid gas, corrosive gas Excessive places. Place the instrument table should be smooth, without

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vibrations; nearby fan of the instrument should be left enough space to exhaust smoothly. Instrument is best to use
a single power outlet, power should ensure good grounding. Doing so may result in equipment not working properly.
If the local voltage instability, give the instrument is equipped with power supply. The instrument should avoid
direct sunlight. Avoid dusty environments.
II. Product features and installation
1. Features
With low stray light and a high resolution structure of double-beam optical monochromator , the instrument has a
good stability, reproducibility and accuracy of readings.
Not only the instrument has automatically set 0% T and 100% T and other control functions as well as the
concentration of computing and data processing functions of a variety of methods, but also to prevent user errors
with special features, no worries when using . Scientific design, the use of new technologies, the optical, mechanical,
electrical and computer technology combined with organic, both to improve product performance and convenience
for users to use.
Large screen graphic LCD display can display the data and maps. The extensive machine software can complete
quantitative analysis, qualitative analysis, kinetics, DNA/Protein and other tests. Coupled with powerful storage and
printing capabilities, the computer can complete all testing, analysis and data output with no connection with PC.
Optional equipment is also available on the Windows platform running UV Professional user application software,
the instrument has greater functionality.

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2. Performance Indicator
Model
C-7100
C-7100S
C-7100A
Display
7 inch TFT
Keyboard Control
Silicone Buttons
Optical System
Single Beam
Holographic grating, 1200 lines/mm
Slit Width
2nm
1 nm
0.5,1,2,4 nm
Wavelength
Range
190 - 1100nm
Wavelength
Accuracy
±0.3nm
Wavelength
Repeatability
≤0.1nm
Photometric
Accuracy
0.2%T(0-100%T), ±0.002A(0-0.5A), ±0.004A(0.5-1A)
Photometric
Repeatability
≤0.15%T(0-100%T), 0.001A(0-0.5A), 0.002A(0.5-1A)
Stray Light
≤0.03%T@220nm,360nm
Stability
±0.001A/h@500nm
Photometric
Range
0-200%T, -0.3-3.0A, 0-9999C(0-9999F)
Baseline Flatness
±0.0015A(200-1000nm)
Noise
0.0003A@500nm
Working Mode
T,A,C,E
Wavelength
Setting
Automatic
Scanning Speed
High, Medium and Low
Detector
Solid Silicon Photodiode
Light Source
Tungsten Halogen/Deuterium Lamp
Data Output
RS232, USB(A), USB(A)
Processor
Cortex_M3, 120Mhz
Power
Requirements
AC 220V/50Hz
Shipping
Dimensions and
Weight
770*630*340mm, 27kg

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3. Main Functions

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4.Structure introduction
Overall structure consists of three parts: optical system, power system and micro-computer system.
1) Top view:
2) Bottom view:

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3) Light path diagram
4) Important part of the instrument
A. Power Board: The 220V50Hz/110V60Hz power conversion and regulation outside access to the necessary
equipment:-11.5V (tungsten lamp power), +12 V (fan power, electrical power), +5 V (computer system power), + /-
15V (signal board power supply), deuterium lamp filament preheating and lit power deuterium lamp is lit up and
the power breakdown.
B. Light room: there are tungsten halogen lamp and deuterium lamp source device to automatically switch.
Halogen tungsten: tungsten halogen light source visible. Applicable wavelength range 340-1900nm. Because it uses
the principle of halogen cycle, it has a greater intensity of light emission and longer life. As the halogen cycle
requires a higher temperature. Thus lamps commonly used quartz glass or high temperature glass. Tungsten
halogen lamps emit energy in the visible region around the operating voltage is proportional to the fourth power,
therefore, to make light stable, the model has a stability of less than 0.2% of the power supply, the other imported
Philips halogen socket tungsten halogen lamp replacement to ensure stable and easy to use and long life.
Deuterium lamp: suitable wavelength range of 190-340nm. When deuterium lamp operation, maximum energy
sources in the vicinity of 230nm, 486.0nm and 656.1nm and has two characteristic lines, can be used for instrument
calibration wavelength accuracy in the visible region. Stability of the steady flow of less than 0.02% of the models,
to ensure stable deuterium lamps, imported from Japan, Hitachi another seat belt deuterium lamp replacement
lamps to ensure the convenience and use of the stability and long life.
C. Monochromator: contains spectral components - grating, into the slit, the slit, mirror, focusing lens, and the
wavelength filter drive system, the monochromatic light is emitted from the composite light can be decomposed
into monochromatic any wavelength of monochromatic light from the optical separation means
Raster: the dispersion of the original, the model uses 1200 / mm holographic grating ensure high resolution and low
stray light.
Filter: Due to the grating spectrum spectral overlap exists between the problem class times, so the use of filters to
eliminate spectral overlap problem.
Action slits, the slits in the monochromator is large, and the resolution of the instrument is not only related to the
dispersion of the grating and the size of the image (i.e., the slit width). The slit is too large, color band is
deteriorated, is not conducive to the qualitative analysis, quantitative analysis also affect the linear range of the
calibration curve, the slit is too small, flux decreased, reducing the signal to noise ratio, affect the measurement

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accuracy, the slits there are two general representation of the width, the actual width of the slit of a knife-edge
between the two expressed (in mm), the other to represent the bandwidth of spectral bands (in nm).
D. Spectroscopic Room: contains a half-mirror, focusing lens and reflector to achieve a beam monochromator out
into two beams of light in different directions
E. Sample Chamber: fixed 4-cell holder. 8-cell changer is optional.
F. Signal Board: Transfer to the motherboard processed signal detection light amplification
G. Main board: instrument micro control unit, control instruments light source switch, the motor rotates, the signal
processor display, etc.
5. Instrument installation
1) After unpacking, carefully check the packing list inside the object is complete and intact;
2) Determine whether the work environment to meet the foregoing requirements, the ambient temperature 10 ~
35 ℃, relative humidity less than 85%, operating voltage (220 3 22) V / (50 3 1) H3
3) The instrument is placed on a horizontal platform, the instrument should avoid direct sunlight and away from
electromagnetic launchers and high-power electrical devices, the use of the environment can not have dust,
corrosive gases and vibration;
4) Around the instrument can not be any obstacle to the flow of air around the instrument;
5) The company supplied with a power cord and make sure there are well-grounded power outlet line;
6) Check the sample chamber, ensure that there is not any solution, and foreign matter and the process of self-test
to ensure that the sample compartment lid is closed, you can not half-open (this is very important,
Otherwise affect the instrument self-test results and normal use!!!).
7) Turn on the instrument. Then the instrument make self-test. after self-test, the instrument can be operated
normally. in case there is an error alarm halfway, please refer to the chapter of instrument troubleshooting.

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III. Key definitions and basic operation
1. Button panel schematic diagram
2. Button function description
Button name Button functional description
【MENU】Menu key under each function
【PRINT】Print output button
【SAVE】File storage button
【LOAD】File open button
【ESC】Back, cancel button, test stop button
【CLEAR】Clear key to delete the input data, delete files
【GOλ】Set the wavelength
【ZERO】Adjust 100%T and 0Abs, build user baseline key
【OK】Enter the confirmation button, function, menu selection button
【START】Test beginning button
【0】-【9】Number button
【.】The decimal point
【+/-】Plus or minus sign
【↑】,【↓】Up and down key
【←】,【→】Left and Right key
【CELL】Automatic sample holder button

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3. Basic operation
1) How to adjust the blank
In any test interface, put the cuvette containing the reference solution into cuvette slot, and pull it into light path.,
press【Zero】key to adjust the blank.
2) How to set the wavelength
In any measurement interface press【Goλ】key to set the current working wavelength
3) Store files in txt or cvs format ( to use Excel format for simple spreadsheet file)
IV. Instrument self-test
1.Communication port inspection
Check the communication port of the instrument is working properly. The result is displayed correct as √ and an
error as ×, the buzzer alarm.
2.File system check
Check the instrument's built-in Flash file system is correct. The result is displayed correctly √, an error will reformat
the file system.
3.Filter positioning
Check the instrument's filter motor and its locator is working correctly. The result is displayed correct as √, an error
as ×, the buzzer alarm.
4.Light positioning
Check the instrument light switch motor and its locator is working correctly. The result is displayed correctly √, an
error is displayed result is ×, the buzzer alarm.

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5.Printer check
Check the printer interface device is working properly. The result is displayed correctly √, an error is displayed result
is ×, the buzzer alarm.
6.Tungsten lamp examination
Open the instrument tungsten light source, check the operating parameters of tungsten lights are working properly.
If the parameter is not working properly, then re-set the operating parameters of a tungsten lamp. Change
detections are always displayed correctly, the result is always √.
7.Deuterium lamp examination
Open deuterium light source instrument operating parameters to check the deuterium lamp is working correctly. If
the parameter is not working properly, then re-set the operating parameters of the deuterium lamp. Change
detections are always displayed correctly, the result is always √.
8.Signal detector check
Check signal detector instrument is working correctly. The result is displayed correctly √, an error is displayed result
is ×, the buzzer alarm.
9.Wavelength Calibration
Wavelength parameter checking instrument is working properly. Correct, then the pop-up boxes, please confirm
whether the user input wavelength calibration if no user input within five seconds, then skip this. If the argument is
wrong wavelength, then start looking for a deuterium lamp to automatically correct the characteristic peak
wavelength. Wavelength calibration result is displayed by √, the correction is not passed, then the result is ×, the
buzzer alarm.
10.Dark current correction
Read instrument dark current of energy, checking eligibility. If the dark current in the proper range, which means
that the dark current is correct, then displays the results of √. If the dark current exceeds the maximum setting, the
user is prompted dark current error. The results are displayed as ×, the buzzer alarm.
11.System parameters check
Instrument system baseline reading is correct. If correct, then the pop-up box that asks the user whether to re-enter the
correction system baseline, baseline correction system default does not automatically skip the 3 seconds. If an error does
not exist or the baseline, the baseline correction system directly. Showing results √, the correction is not
passed, then the result is ×, the buzzer alarm.
Self-test end, after re-calibration dark current, into the main instrument display.

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Note: After power on the instrument, the instrument will automatically self-test and initialization until after
initialization is complete, the instrument will warm up for 20 minutes, 20 minutes warm-up time or press [ESC] to
skip preheating case, the instrument being prompted preparatory work environment, which is the instrument re-
calibration dark current, set the working parameters, etc., and then enter the main menu.
V. Photometric measurement
1. Function description
Photometric measurements are measuring the absorbance of the sample at a single wavelength, transmittance or
energy value.
2. Set measuring mode
Press the set button, enter the measurement mode settings menu, select the desired test mode, press [ENTER] to
confirm. If you choose the energy model, energy window appears and prompts to select the amplifier gain.

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3.Set wavelength
Set the current working wavelength range 190nm-1100nm, press the up and down button to open wavelength
setting window, press [0] - [9] to enter the desired wavelength, press [CLEAR] to clear the input, press [ENTER] to
confirm. Input errors or exceeds the set range buzzer alarm.
4. Correction 100%T/0Abs
In two samples simultaneously into the two slots reference solution, and then press the [ZERO] key, the instrument will
be blank correction in the current wavelength. Display calibration is completed 100.0% T or 0.000Abs.
5. Measurement data
Calibrated with reference solution 100% T/0Abs, remove the rear slot reference sample solution into the sample solution,
and then press the [START] button, then perform a test, the sample data are immediately added to the list.

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6. Delete data
Delete files Press [CLEAR] button, delete files prompt box will pop up, this operation will delete all the test data
currently under test. Select [Yes], the file will be deleted all the data, select [No] will return the test window.
7. Save the file
Press the Save button to save the current list of test data to a file. If this is the first time you save a file, the dialog
box will pop up asking to save the file name input.

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8. Open the file
In photometric measurement interface will be displayed by the Load button to open the window, open the window
has all the photometric data file list. Select the appropriate data file and press [ENTER], the file will be read into all
the test data, and then automatically enters the measurement mode.
VI. Quantitative measurement
Select [quantitative measurement] with up and down, left and right key, then press Enter key. there are two
options: build standard curve and open standard curve.