PerkinElmer Wallac 1420 User manual

1420-922-07
March 2003
User manual
1420
Software version 3.00
PerkinElmer Life and Analytical Sciences, Wallac Oy, P.O. Box 10, FIN-20101 Turku, Finland.
Tel: [phone]. Fax: [phone]. Website: www.perkinelmer.com/lifesciences

Contents
1
Contents
Introduction .......................................................................................................................... 9
System overview............................................................................................................... 11
Enhanced Security mode .................................................................................................. 12
Software installation ......................................................................................................... 13
Operation overview .......................................................................................................... 14
Operating Wallac 1420....................................................................................................... 15
Starting up Wallac 1420 ................................................................................................... 17
Without enhanced security mode.................................................................................. 17
With enhanced security mode....................................................................................... 17
Instrument control............................................................................................................. 18
Live display ...................................................................................................................... 19
Temperature...................................................................................................................... 21
Barcode options ................................................................................................................ 22
Plate orientation................................................................................................................ 22
Starting operation with the Start button............................................................................ 23
Starting operation with the Start Wizard .......................................................................... 26
Starting operation and using a protocol number barcode ................................................. 28
Stacker operation .............................................................................................................. 29
Starting operation, no protocol number barcode .......................................................... 29
Starting operation and using a protocol number barcode ............................................. 31
Ending operation............................................................................................................... 32
Stop button.................................................................................................................... 32
End button..................................................................................................................... 32
Button on Wallac 1420 ..................................................................................................... 33
Light on the button ....................................................................................................... 33
Protocol editing ................................................................................................................... 35
Explorer ............................................................................................................................ 37
Explorer folders ............................................................................................................ 38
Explorer icons............................................................................................................... 38
Protocol selection.......................................................................................................... 39
Protocol editor .................................................................................................................. 40
Samples............................................................................................................................. 41
ID...................................................................................................................................... 43
Measurement .................................................................................................................... 44
Measurement mode....................................................................................................... 44
Measurement operations............................................................................................... 45

Contents
2
Down button ............................................................................................................. 45
Up button .................................................................................................................. 45
Delete button............................................................................................................. 45
Dispense button......................................................................................................... 45
Delay button.............................................................................................................. 46
Shake button ............................................................................................................. 46
Label button .............................................................................................................. 46
Kinetics button .......................................................................................................... 47
Scanning button ........................................................................................................ 47
Injector position during measurement....................................................................... 47
Plate .................................................................................................................................. 48
Plate repeat parameters ................................................................................................. 48
Measurement height...................................................................................................... 48
Plate type....................................................................................................................... 49
Temperature level checking .......................................................................................... 49
Outputs.............................................................................................................................. 50
Events................................................................................................................................ 52
Keywords ...................................................................................................................... 53
General.............................................................................................................................. 54
File menu .......................................................................................................................... 55
Save............................................................................................................................... 55
Print............................................................................................................................... 55
Print preview................................................................................................................. 55
Print setup .....................................................................................................................55
Start............................................................................................................................... 55
Exit................................................................................................................................ 55
Tools menu ....................................................................................................................... 56
Result viewing ..................................................................................................................... 57
Latest results ..................................................................................................................... 59
Results in the Explorer...................................................................................................... 59
List ................................................................................................................................ 61
Plate map.......................................................................................................................62
Protocol......................................................................................................................... 63
Notes ............................................................................................................................. 64
Error .............................................................................................................................. 64
Print............................................................................................................................... 64
Export results ................................................................................................................ 64

Contents
3
Tools menu .......................................................................................................................... 65
Tools menu ....................................................................................................................... 67
User level.......................................................................................................................... 68
Labels ............................................................................................................................... 69
Time-resolved fluorometry ........................................................................................... 71
Protocol name ........................................................................................................... 71
Flash Energy Area .................................................................................................... 72
Flash Energy Level ................................................................................................... 72
Excitation Filter ........................................................................................................ 72
Light Integration Capacitors ..................................................................................... 72
Note .......................................................................................................................... 73
Light Integration Reference Level............................................................................ 73
Emission filters ......................................................................................................... 73
Emission aperture ..................................................................................................... 73
Counting parameters................................................................................................. 74
Factory-set values for the main counting window.................................................... 74
Beam size.................................................................................................................. 74
Second measurement parameters.............................................................................. 74
Fluorometry .................................................................................................................. 75
Protocol name ........................................................................................................... 75
CW-Lamp energy ..................................................................................................... 76
CW-Lamp Control Mode.......................................................................................... 76
CW-Lamp filters....................................................................................................... 76
Excitation aperture (Wallac 1420-040/041/042/043 only) ....................................... 76
Emission filters ......................................................................................................... 76
Emission aperture ..................................................................................................... 77
Counter position ....................................................................................................... 77
Counting time ........................................................................................................... 77
Second measurement parameters.............................................................................. 77
Photometry.................................................................................................................... 78
Protocol name ........................................................................................................... 78
Absorbance mode ..................................................................................................... 78
Flash Lamp Filter...................................................................................................... 78
CW-Lamp filters....................................................................................................... 79
Excitation aperture (Wallac 1420-040/041 only) ..................................................... 79
Reading time............................................................................................................. 79
Second measurement ................................................................................................ 79
Luminometry ................................................................................................................ 80

Contents
4
Protocol name ........................................................................................................... 80
Emission filters ......................................................................................................... 80
Emission aperture...................................................................................................... 81
Counting time............................................................................................................ 81
Second Measurement ................................................................................................ 81
Fluorescence polarization (Wallac 1420-040/041/042/043 only)................................. 82
Protocol name ........................................................................................................... 82
CW-Lamp energy...................................................................................................... 83
CW-Lamp filter......................................................................................................... 83
Polarizer aperture ...................................................................................................... 83
Emission filter........................................................................................................... 83
Emission aperture...................................................................................................... 83
Counting time............................................................................................................ 83
G factor ..................................................................................................................... 84
LANCE ......................................................................................................................... 85
Protocol name ........................................................................................................... 85
Flash Energy Area..................................................................................................... 86
Flash Energy Level ................................................................................................... 86
Excitation filter ......................................................................................................... 86
Light integration capacitors ...................................................................................... 86
Note........................................................................................................................... 87
Light Integration Reference Level ............................................................................ 87
Emission filter........................................................................................................... 87
Emission aperture...................................................................................................... 87
Counting parameters ................................................................................................. 88
Factory-set values for the main counting window .................................................... 88
Label ......................................................................................................................... 88
Delay 1 ...................................................................................................................... 88
Delay 2 ...................................................................................................................... 88
Window 1.................................................................................................................. 88
Window 2.................................................................................................................. 88
Cycle ......................................................................................................................... 88
Beam size .................................................................................................................. 88
Second measurement parameters .............................................................................. 88
LANCE Label Properties.......................................................................................... 89

Contents
5
Filters................................................................................................................................ 90
Emission filters ............................................................................................................. 91
CW-Lamp filters........................................................................................................... 92
Filter slides ...................................................................................................................93
Emission filter slide .................................................................................................. 94
Physically changing the emission filter slide............................................................ 95
CW-Lamp filter wheel.............................................................................................. 96
Physically changing the CW-Lamp filter wheel....................................................... 97
Changing the CW-Lamp........................................................................................... 97
Changing filters in the software................................................................................ 98
Filter properties......................................................................................................... 99
EuSm Dual label normalization...................................................................................... 100
Plate type selection ..................................................................................................... 100
Preparing the plate ...................................................................................................... 101
Measurement status page............................................................................................ 102
Measurement warning page........................................................................................ 102
Normalization confirmation page ............................................................................... 103
LANCE normalization.................................................................................................... 104
Using the LANCE normalization wizard.................................................................... 104
Protocol selection........................................................................................................ 105
Preparing the plate ...................................................................................................... 106
Measurement status page............................................................................................ 107
Measurement warning page........................................................................................ 108
Normalization confirmation page ............................................................................... 109
Using LANCE normalization samples in the assay.................................................... 110
Plate dimension wizard................................................................................................... 112
Plate selection ............................................................................................................. 113
Technology selection .................................................................................................. 113
Preparing the plate ...................................................................................................... 114
Define the centre point of the corner wells................................................................. 115
Miscellaneous settings .................................................................................................... 117
Plate type .................................................................................................................... 119
Plate name............................................................................................................... 119
Number of well rows .............................................................................................. 120
Number of well columns......................................................................................... 120
Height of the plate .................................................................................................. 120
Strip orientation ...................................................................................................... 120
Plate dimensions ..................................................................................................... 120

Contents
6
Tune ........................................................................................................................ 120
Well names.................................................................................................................. 120
Instrument properties .................................................................................................. 120
Options............................................................................................................................ 121
General........................................................................................................................ 121
Instrument hardware ................................................................................................... 124
Measurement technologies.......................................................................................... 125
Exiting................................................................................................................................ 127
File menu ........................................................................................................................ 129
Automatic logout ........................................................................................................ 129
Troubleshooting ................................................................................................................ 131
Out of memory................................................................................................................ 133
Display problems ............................................................................................................ 134
Performance degradation ................................................................................................ 134
Processing error .............................................................................................................. 135
System requirements not met.......................................................................................... 135
Appendices......................................................................................................................... 137
Appendix 1: Connecting MultiCalc to Wallac 1420....................................................... 139
Introduction................................................................................................................. 139
Wallac 1420 MultiCalc assay protocols...................................................................... 139
Installation of Wallac 1420 output option in MultiCalc ............................................. 140
MultiCalc assay protocols........................................................................................... 140
Usage of MultiCalc ..................................................................................................... 140
Appendix 2: Plate types for stackers............................................................................... 141
Wallac ..................................................................................................................... 141
Nunc........................................................................................................................ 141
Costar ...................................................................................................................... 141
Greiner .................................................................................................................... 141
Appendix 3: Dispenser operation.................................................................................... 143
Introduction................................................................................................................. 143
Emptying the waste vial.............................................................................................. 144
Preparing the tubing.................................................................................................... 146
Dispenser maintenance ............................................................................................... 147
Measurement............................................................................................................... 150
Maintenance................................................................................................................ 151
Needle maintenance ................................................................................................ 151

Contents
7
Appendix 4: Enhanced security special functions .......................................................... 153
User groups and permissions...................................................................................... 153
User Management....................................................................................................... 154
Password Control........................................................................................................ 156
Audit Trail .................................................................................................................. 158
Archive ....................................................................................................................... 160
Security Settings ......................................................................................................... 161
Index .................................................................................................................................. 165
Trademarks
DELFIA is a registered trademark and LANCE is a trademark of PerkinElmer, Inc.
Windows and Excel are registered trademarks of Microsoft Corp. in the U.S. and other
countries.

9
Introduction

10

Introduction
11
System overview
Note: This manual describes all the features of Wallac 1420. Some versions of the
instrument do not have one or more of these features. You can ignore the parts of this
manual not relevant to the version of Wallac 1420 you have.
Wallac 1420 multilabel counter is a complete platform for quantitative detection of light-
emitting, or light absorbing markers. Depending on the model of the instrument you have, it
is suitable for flash or glow luminometry, fluorometry, fluorescence polarization, high-
sensitivity time-resolved fluorometry, homogeneous time-resolved fluorometry (LANCE
option) and photometry. It is a compact bench top unit with features such as dispensers,
shaking, temperature control, top or bottom reading and scanning. The software is a 32-bit
application running under Windows2000 or Windows XP. Up to 1536-well plates can be
counted (depending on the technology and model) as well as Petri dishes, slide filters and
Terasaki plates. Plates can be loaded manually or with stackers for automatic operation.
Output can be to a file on the PC and/or to a laser printer. The following figure shows the
Wallac 1420 system with its PC and laser printer.
If the dispenser unit is connected to Wallac 1420 it looks as follows:

Introduction
12
Enhanced Security mode
Wallac 1420 has an Enhanced Security mode intended for facilities that have to comply
with 21 CFR Part 11 regulation from the Food and Drug Administration (FDA) of the USA.
During installation you can select if you want to use the Enhanced Security mode. Once you
have enabled this mode you cannot disable it because to do so would not be in compliance
with 21 CFR Part 11.
The regulation (21 CFR Part 11) sets the criteria under which the agency (FDA) considers
Electronic Records and Electronic Signatures (if applied) to be trustworthy, reliable and
generally equivalent to traditional paper records and hand-written signatures executed on
paper. The regulation applies to records in electronic form that are created, modified,
maintained, archived, retrieved, or transmitted, under any records requirements set forth in
agency regulations (i.e. GMP, GLP, GCP…). It also applies to electronic records submitted
to FDA under requirements of the Federal Food, Drug, and Cosmetic Act.
The Enhanced Security mode for Wallac 1420 provides the technological controls and
features to support full 21 CFR Part 11 compliancy. These features can be classified under
three main headings:
•improved access control (unique Userid/Password combination) with five user
levels
•improved data security
•audit trails of user actions
This manual and the electronic help will tell you how to use Wallac 1420 with or without
the Enhanced Security mode. All differences in use are noted and explained.
Note: use of the Enhanced Security mode does not alone ensure compliance with 21
CFR Part 11. Any facility that wants to be compliant with the 21CFR Part 11
regulation must also implement the necessary procedures and controls set by the
regulation.
Please refer to Appendix 4 for details of special functions that are part of the Enhanced
Security mode.

Introduction
13
Software installation
Note: the following instructions assume that the counter, PC and printer have been
connected up and switched on (see the installation instructions in the Instrument manual). If
Enhanced Security mode is used then installation can only be done by a person with System
and Windows administrator rights. See Appendix 4 for more details.
1. Load the software CD.
Load CD
2. The setup loader will start automatically and give you a choice of either selecting the
Installation Wizard to guide you through the installation or reading files from the CD.
3. When you have completed the installation you should remove the CD from the PC,
otherwise the setup loader will start again next time you boot the PC.
Note: in the case of Windows XP the user has to have “power user” rights.

Introduction
14
Operation overview
Parameters must first be set using the protocol editor. This is activated by selecting a
protocol in the Explorer.
A sample plate can then be loaded and measured using the protocol set for it.
Other parameters affecting the whole system e.g. labels, filters etc. can be set in the Tools
menu.

15
Operating Wallac 1420

16

Operating Wallac 1420
17
Starting up Wallac 1420
Without enhanced security mode
When you boot the system the following window appears:
With enhanced security mode
When the Enhanced Security mode is installed the login dialogue appears after you have
started Wallac 1420.

Operating Wallac 1420
18
Type the appropriate userid/password combination to proceed. Clicking the Cancel button
closes Wallac 1420. Clicking the Change password opens the Change Password dialogue
where you can define a new password.
After a successful login Wallac 1420 can be used according to the permissions granted to
the logged on user.
Instrument control
The main window has three tabs: Instrument Control, Live Display and Temperature. The
instrument control tab enables you to control the instrument by starting, stopping or ending
counting. It shows you the protocol being used to control the measurement and it also shows
status of the instrument.
In the enhanced security mode these controls are enabled only for users who belong to the
Operator user group.
If the system supports plate heating, the status bar will display the current temperature of the
plate and samples. When run with a system not supporting plate heating this will not be
displayed.