PerkinElmer IVIS Lumina S5 User manual

PN CLS149599
IVIS®Lumina S5
January 2018
HARDWARE MANUAL

PerkinElmer Health Sciences
940 Winter Street, Waltham, Massachusetts 02451 USA
Telephone: [phone] (US) or +1.203.925.4602
Fax: [phone]
For sales: [email protected]
For Technical Support: [email protected]
877.522.497.3302 (US) or +1.508.435.9500
www.PerkinElmer.com
Trademarks
Discovery in the Living Organism, IVIS and Living Image are either registered trademarks or trademarks of PerkinElmer Health
Sciences, Inc. and/or its parent, affiliates, and/or subsidiary companies (collectively "PerkinElmer"). The names of companies
and products mentioned herein may be the trademarks of their respective owners. Apple, Macintosh and QuickTime are
registered trademarks of Apple Computer, Inc. Microsoft, PowerPoint and Windows are either registered trademarks or
trademarks of Microsoft Corporation in the United States and/or other countries. Adobe and Illustrator are either registered
trademarks or trademarks of Adobe Systems Incorporated in the United States and/or other countries. Reproduction of this
publication or parts thereof in any form is expressly prohibited without the express written permission of PerkinElmer. Any
errors or omission which may have occurred in this publication despite the utmost care taken in its production will be
corrected as soon as possible, but not necessarily immediately upon detection. PerkinElmer provides this publication "As Is"
without warranty of any kind, either express or implied, including but not limited to the implied warranties of merchantability
or fitness for a particular purpose. Some states or jurisdictions do not allow disclaimer of express or implied warranties in
certain transactions; therefore, this statement may not apply to you. PerkinElmer reserves the right to revise this publication
and to make changes from time to time in the content hereof without obligation of PerkinElmer to notify any person of such
revision or changes
Copyright
© 2018
PerkinElmer Health Sciences, Inc. and its parent, affiliated, and subsidiary companies. All rights reserved, including but not
limited to those rights to reproduce this publication or parts thereof.

Contents
Chapter 1 Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Contact Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Chapter 2 Important Safety Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.1 Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2.2 Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Read, Retain, and Follow Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Heed Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.3 Emergency Stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.4 Environmental Considerations for the System Components . . . . . . . . . . . . . 4
Location for the IVIS Lumina S5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Heat . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Water and Moisture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.5 Cleaning or Moving the System Components . . . . . . . . . . . . . . . . . . . . . . . . 5
Cleaning/Liquid Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Moving the IVIS Lumina S5 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.6 Power Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power Sources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power Cord Protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Power Outages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Overloading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.7 Servicing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.8 Other Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Chapter 3 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.1 Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.2 Mechanical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3 Chemical and Biological Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
3.4 Electronics Tray and Enclosure, System Cover . . . . . . . . . . . . . . . . . . . . . . . . 8
3.5 Korean EMC Registration Statement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Chapter 4 Legal Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.2 Limited Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.3 Patents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.4 Trademarks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.5 Disclaimers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Chapter 5 Components and Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
5.2 CCD Camera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

IVIS®Lumina S5 Hardware Manual Contents ii
CCD Camera Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
CCD Camera Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5.3 Imaging Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Imaging Chamber Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Imaging Chamber Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.4 Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.5 Filter Wheels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.6 Acquisition Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Computer Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.7 Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Chapter 6 Basic Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.1 Starting the IVIS Lumina S5 Imaging System . . . . . . . . . . . . . . . . . . . . . . . . 18
6.2 Electrical Connections and Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Electrical Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Gas Plumbing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.3 Door Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.4 Stage Dock and Rodent Manifold for Anesthesia . . . . . . . . . . . . . . . . . . . . . 21
6.5 Imaging Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Black Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Centering a Subject . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Glowing Materials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.6 Restarting the System After a Power Outage . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
6.7 System Shut Down Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Chapter 7 Care and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
7.1 Cleaning and Disinfecting the Imaging System . . . . . . . . . . . . . . . . . . . . . . 24
7.2 Camera Coolant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Checking the Coolant Level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Chapter 8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
8.1 Measured Temperature Is Not Equal to Demand Temperature . . . . . . . . . . 27
8.2 Photographic Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
8.3 Luminescent Image Is Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
8.4 No Image Produced . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Appendix A Moving the Imaging System on an IWB Workbench . . . . . . . . . . . . . . . . . . . 30
A.1 Shutting Down the Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
A.2 Moving the Imaging System on an IWB Workbench . . . . . . . . . . . . . . . . . . 30
A.3 Starting the Imaging System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Appendix B Options and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

1 Welcome
Introduction
Contact Information on page 2
1.1 Introduction
The IVIS Lumina S5 is a high-sensitivity, low-noise imaging system enabling both fluorescent and
bioluminescent imaging in vivo (Figure 1.1). The imaging system is compatible with the Smart High
Throughput Imaging and ID Kit (CLS148875) and the MVI-2 Imager for multi-view imaging.
The IVIS Lumina S5 Imaging System includes:
A highly sensitive CCD camera.
A light-tight imaging chamber with integrated fluorescence module.
Fluorescence equipment:
22-position excitation filter wheel with 19 equally spaced excitation filters covering the
wavelength range from 410 to 790 nm
8-position emission filter wheel with 7 filters spanning the wavelength range from 500 – 865 nm
Living Image®Software for automated image acquisition, post-processing, and data analysis.
A Windows®-based computer system for data acquisition and analysis.
This manual explains how to operate and maintain the equipment, and provides guidelines for
obtaining optimal bioluminescent and fluorescent images. Before using the IVIS Lumina S5, please
read this manual carefully to obtain safe, optimum performance and a maximum service life from the
instrument. For instructions on using the Living Image software, please see the Living Image®
Software User Manual for IVIS Lumina S5.
Figure 1.1 IVIS Lumina S5

IVIS®Lumina S5 Hardware Manual Chapter 1 | Welcome 2
1.2 Contact Information
If you have questions regarding this manual or the IVIS Lumina S5, please contact PerkinElmer
Technical Support.
PerkinElmer Health Sciences
940 Winter Street
Waltham, Massachusetts 02451 USA
www.PerkinElmer.com
Technical Support
Telephone: [phone] (US) or [phone]
Fax: [phone]
E-mail: [email protected]
Sales: [email protected]

2 Important Safety Instructions
Safety Information
Instructions
Emergency Stop on page 4
Environmental Considerations for the System Components on page 4
Cleaning or Moving the System Components on page 5
Power Considerations on page 5
Servicing on page 6
Other Equipment on page 6
2.1 Safety Information
This manual provides safety information in the following formats:
2.2 Instructions
Read, Retain, and Follow Instructions
Read and understand all the safety and operating instructions before you install, operate, or perform
maintenance on the IVIS Lumina S5. Make sure that you fully understand the following safety
instructions, warnings, and disclaimers before proceeding to the rest of the manual.
Retain the safety and operating instructions for future reference.
Follow all operating and handling instructions. Failure to follow operating or handling instructions
may void any warranty covering this product.
!
CAUTION: A caution note indicates a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury and/or mechanical damage. It is
also used to alert you to unsafe practices. It reminds you that all safety
instructions should be read and understood before installation, operation,
maintenance, or repair of this instrument. When you see this symbol, pay
particular attention to the safety information presented. Observance of safety
precautions will help avoid actions that could damage or adversely affect the
performance of the IVIS Lumina S5. If the equipment is used in a manner not
specified in this manual, the protection provided by the equipment may be
impaired.
!
WARNING! Used when an action or condition may potentially cause serious
personal injury or loss of life. Mechanical damage may also result.
VOLTAGE! Provides safety information about high voltage or risk of electric
shock.

IVIS®Lumina S5 Hardware Manual Chapter 2 | Important Safety Instructions 4
Heed Warnings
Abide by all warnings on the product and in the operating instructions. Failure to adhere to warnings
or safety precautions may void any warranty covering the IVIS Lumina S5.
2.3 Emergency Stop
The emergency stop button at the lower front of the instrument provides electro-mechanical safety.
Push in the emergency stop button to immediately stop shelf motion.
Reset the emergency stop button by twisting it clockwise.
2.4 Environmental Considerations for the System Components
Location for the IVIS Lumina S5
Before the IVIS Lumina S5 is installed, consider the proper environment for the components. Install
the equipment in an environment where:
The temperature does not fluctuate widely and is maintained between 15-25 °C (59-77 °F).
The humidity does not exceed 80%.
No strong electric or magnetic fields exist.
No vibrations are present.
No corrosive gases are present.
High amounts of dust are not present.
No open flame is present.
There is sufficient space around the imaging system, including:
A minimum space of four inches on either side of the instrument for unobstructed air flow.
Eighteen inches of clearance on the right-hand side of the instrument for service access.
The work space is level.
Heat
The system should be situated away from heat sources such as open flames, radiators, heat registers,
stoves, and other heat-generating electrical equipment.
Figure 2.1 Emergency Stop Button
Emergency stop button

IVIS®Lumina S5 Hardware Manual Chapter 2 | Important Safety Instructions 5
Water and Moisture
2.5 Cleaning or Moving the System Components
Cleaning/Liquid Entry
Moving the IVIS Lumina S5
2.6 Power Considerations
Power Sources
The IVIS Lumina S5 uses a universal power supply that can accept multiple voltages. There is no
need to configure the system for a particular voltage.
Power Cord Protection
Power supply cords should be routed so that they are unlikely to be walked on or pinched by items
placed upon or against them. Pay close attention to receptacles and to points of connection between
cords and equipment.
Power Outages
If the IVIS Lumina S5 experiences a loss of supply power, turn off the power switches for all
components and do not restart the system until reliable power has been restored.
VOLTAGE! Do not use this product near water (for example, near a sink or wet
room) due to risk of electric shock, electrical damage, and/or equipment failure.
VOLTAGE! Do not use liquid or aerosol cleaners and never spill liquid of any kind
on any of the imaging system components. Sprays and liquids that come into
contact with the IVIS Lumina S5 hardware may result in damage to the system or
electrocution. For more details on proper care of the system, see Care and
Maintenance on page 24.
!
CAUTION: The IVIS Lumina S5 is sensitive, scientific equipment and should not be
moved by any user unless the system is located on an IWB Workbench. Due to the
risk of potential damage, it is critical that only a trained PerkinElmer technician
moves the IVIS Lumina S5. If it is necessary to relocate the imaging system,
contact PerkinElmer Technical Support (see page 2). See Appendix A on page 30
for more details on moving the IVIS Lumina S5 on an IWB Workbench.

IVIS®Lumina S5 Hardware Manual Chapter 2 | Important Safety Instructions 6
Overloading
Facilities should be adequately wired according to local building codes.
2.7 Servicing
Refer all servicing to PerkinElmer Technical Support (see page 2). If the IVIS Lumina S5 is damaged
and requires service, unplug the instrument from the outlet and contact PerkinElmer Technical
Support. Servicing by anyone other than an authorized PerkinElmer representative voids the
warranty covering the IVIS Lumina S5.
2.8 Other Equipment
Use of any equipment other than that recommended by this manual has not been evaluated for safety
and, therefore, is the sole responsibility of the user.
!
WARNING! Do not overload wall outlets or extension cords as this can result in a
risk of fire or electric shock. See Components and Specifications on page 13 for
more details on the power requirements of the equipment.