PerkinElmer VICTOR Nivo User manual

VICTOR NIVO®
User Manual
(English)

Release History
Part Number Revision Language Publication Date
HH35940301 D en 2022-11-28
This manual describes the VICTOR Nivo Multimode Reader (HH3500), optionally with dispenser
module (HH35000700) and/or stacker module (HH35000800).
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages
in connection with furnishing, performance or use of this material.
Copyright Information
This document contains proprietary information that is protected by copyright. All rights are
reserved. No part of this publication may be reproduced in any form whatsoever or translated into
any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2022 PerkinElmer, Inc.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as
such, are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.
VICTOR Nivo is registered trademark of PerkinElmer Inc.
Google Chrome is a trademark of Google, Inc.
Microsoft Edge is a trademark and Windows and Excel are registered trademarks of Microsoft
Corp.
Service Contact PerkinElmer
Information: https://www.perkinelmer.com
Support: https://www.perkinelmer.com/contactus#/arrange-service-call
Contact address
World Headquarters
PerkinElmer, Inc 940 Winter Street
Waltham, Massachusetts 02451, USA
[phone]
Service Contact MyAssays
Knowledge Base: http://www.myassays.com
Email: [email protected]

Contents
1 Safety 6
1.1 Safety Symbols 6
1.2 Usage of the VICTOR Nivo 7
1.3 Environmental Conditions 8
1.4 Safety Instructions and Warnings 9
1.4.1 Electrical Hazard 9
1.4.2 Hot Surfaces 9
1.4.3 Spillage of Liquids 10
1.4.4 Mechanical Hazards 11
1.4.5 Gas 12
1.4.6 UV Radiation 12
1.4.7 Dangerous Substances 12
1.5 Laser Radiation 13
1.6 Instrument Type Labels 14
2 Introduction 15
2.1 Instrument Overview 15
2.2 Measurement Technologies 15
2.3 Contents of Delivery 15
3 Reader Installation 17
3.1 Hardware Requirements 17
3.1.1 Computer Hardware 17
3.1.2 Control Device Power Options 17
3.2 Software Requirements 18
3.3 Reader Positioning 19
3.4 Power and Communication Connections 19
3.5 Turning the Reader On and Off 20
3.6 Computer Configuration and Connecting the Reader 20
3.6.1 Installation with Direct LAN Cable Connection 20
3.6.2 Installation with Wi-Fi Router 23
3.6.3 Making Reader IP Address a Trusted Site 25
3.7 Software Update 27
4 Initializing the Reader 29
5 Dispenser Module (Optional) 31
5.1 Prime 32
5.2 Wash 33
5.3 Empty Syringe 34
6 Gas Control (Optional) 36
6.1 Gas Connectors 36
6.2 Installation 37
6.2.1 Safety Instructions 37
6.2.2 Supplied Equipment 38
6.2.3 Gas Controller (Rear View) 40

6.2.4 Installing Tubes Between Gas Tanks and Gas Controller 41
6.2.5 Installing Tubes Between Gas Controller and VICTOR Nivo reader 42
6.3 Setting the Pressure 42
6.4 Checking the System and Switching on the Gas Controller 44
6.4.1 Check Cables and Tubing 44
6.4.2 Check Basic Settings of Gas Controller 44
6.5 Gas Controller Configuration 45
6.5.1 Setting Gas Concentrations 45
6.5.2 Control with CO2 Only 45
7 Stacker Module (Optional) 47
7.1 Overview 47
7.2 Positioning 48
7.3 Power and Communication Connections 48
7.4 Turning the Stacker On and Off 49
7.5 Safety Features 50
7.6 Operation via Software 51
7.7 Control Panel 51
7.7.1 Control Buttons 51
7.7.2 Status LEDs 52
7.8 Magazines 53
7.9 Transport 54
8 Retooling 55
8.1 Changing Optical Components 55
8.1.1 Loading a Filter 55
8.1.2 Unloading a Filter 56
8.1.3 Changing a Mirror Slide 57
8.1.4 Installing New Mirrors in a Mirror Slide 59
9 Maintenance 62
9.1 Removing Dust 62
9.2 Cleaning Spillage 62
9.3 Cleaning Optical Components 62
9.4 Changing the Air Filter 63
9.5 Dispenser Maintenance 64
10 Specifications 65
10.1 Reader Specifications 65
10.1.1 General 65
10.1.2 Dimensions 66
10.1.3 Instrument Models 66
10.1.4 Performance 66
10.1.5 Dispenser (Optional) 67
10.1.6 Alpha Laser 67
10.1.7 Electrical Requirements 67
10.1.8 IT 68
10.2 VICTOR Nivo Test Plate (Optional) 69
10.3 Stacker Module (Optional) 70
10.3.1 General 70

10.3.2 Barcode Scanner 71
10.3.3 Stacker Speed 72
10.4 Plate and Lid Compatibility 73
10.4.1 General 73
10.4.2 Stacker 73
11 Index 74

1 Safety
1.1 Safety Symbols
Danger!
Indicates a hazardous situation which, if not avoided, can result in death
or irreversible injury.
Warning!
Indicates a hazardous situation which, if not avoided, can result in severe
but normally reversible injury.
Caution!
Indicates a hazardous situation which, if not avoided, can result in pain or
minor injury
Notice
Failure to observe may result in invalid measurement results or damage
of the instrument
Notice
Useful hint or explanation
Biohazard
Electrical voltage
Hot surface
Flammable material
Ultraviolet (UV) radiation
Corrosive material
Toxic material
Laser radiation
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1.2 Usage of the VICTOR Nivo
Caution!
Use only as intended.
lThe VICTOR Nivo system, including the original accessories may only
be used in conjunction with the methods described in the provided
manuals. PerkinElmer does not assume any liability for any other
applications or procedures, including use of individual subassemblies
or components for other purposes.
lThe manufacturer does not assume any liability for any other kind of
application, including individual subassemblies and the addition of
individual components. This exclusion of liability also applies to all
service or repair work which was not carried out by authorized
PerkinElmer service personnel.
For research use only. Not for clinical diagnostic use.
The VICTOR Nivo is an automated multimode plate reader for use in biological and
pre-clinical research only. The VICTOR Nivo is particularly suited for cellular and
biochemical research applications.
Stacker module
The intended use of the optional stacker module is the automation of microplate
measurements with the VICTOR Nivo multimode reader. Basic stacker operations
can be accessed via the control panel of the stacker. Measurements using the
stacker are controlled via the control software of the reader. For stacker
measurements, microplates may only be added to or removed from the system via
the stacker magazines.
1 Safety
VICTOR Nivo – User Manual 7

1.3 Environmental Conditions
lIndoor use only, not exposed to direct sunlight.
The instrument should not be located in areas where the temperature varies
strongly up and down. This might affect the results.
lAltitude: Up to 2000 m
lOperating conditions:
+15°C to +35°C (ideal: 22 °C), relative humidity 80% maximum at 31°C,
decreasing linearly to 50% at 40°C
lTransportation and storage conditions:
-20°C to +50°C, relative humidity 5 to 90%,
IEC 68-2-56 guidelines provided in transportation package
lInstallation category (overvoltage category): II, according to IEC 60664-1 (see
note)
lPollution degree: 2 according to IEC 60664-1 (see note)
lInstallation category (overvoltage category) defines the level of
transient overvoltage which the instrument is designed to withstand
safely. It depends on the nature of the electricity supply and its
overvoltage protection means. CATII is the category used for
instruments in installations supplied from supply comparable to public
mains such as hospitals and research laboratories and most industrial
laboratories.
lPollution degree describes the amount of conductive pollution present
in the operating environment. Pollution degree 2 assumes that normally
only non-conductive pollution such as dust occurs with the exception of
occasional conductivity caused by condensation.
lBoth of these affect the dimensioning of the electrical insulation within
the instrument.
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1.4 Safety Instructions and Warnings
The VICTOR Nivo system and its optional components must be installed
and used according to the instructions given by the manufacturer. The
service must be done by properly trained personnel authorized by the
manufacturer. The violation of these instructions may result in warranty
invalidation or malfunction of the equipment.
The VICTOR Nivo system may only be operated in a laboratory
environment and by qualified laboratory staff who have been specially
trained and are familiar with the contents of this manual. The user must
implement common laboratory safety standards for protective clothing and
handling and disposing of potentially biohazardous, corrosive and toxic
materials. This device is designed for analyzing biological substances at
biosafety level 1 and 2 (BSL-1 or BSL-2).
All maintenance procedures other than those mentioned in this manual
must be performed by authorized service personnel.
1.4.1 Electrical Hazard
Use only the external power supply unit and power cord supplied with the
reader (and the stacker module, if applicable). The user must not handle
the external power supply or cord if it is damaged. The user must ensure
that the power supply cord can be easily unplugged, if electric safety is
compromised, for example due to a spill. The power cord may only be
connected to the properly installed protective grounding socket.
It is recommended to replace the air filter on the back of the reader once a
year. Switch off the reader and unplug from mains before changing the
filter.
1.4.2 Hot Surfaces
After warming the plate reader, care should be taken when removing the
plate from the reader: the tray and plate may be too hot to handle
barehanded. The heating elements inside the reader may also be hot.
There is a hot surface warning label behind the plate loading door (see
picture below).
1 Safety
VICTOR Nivo – User Manual 9

1.4.3 Spillage of Liquids
Warning! Ethanol or other cleaning solvents may cause fire or injury if
used in great amounts. Keep the concentration of dangerous solvents as
low as possible and ensure the area is well ventilated.
The user must consult the manufacturer prior to using cleaning or
decontamination agents, if there is any doubt about their suitability or
compatibility.
Warning! Do not handle large amounts of liquids near or above the device
and ensure the microtiter plate is correctly placed in the plate holder. If
liquids should be spilled into the instrument accidentally, switch it off
immediately and clean and decontaminate it, if needed. Clean any spillage
leaking out of the instrument with adequate safety precautions, if the liquid
is biohazardous, corrosive or toxic. Ensure the spillage has not
accumulated inside the instrument and the safe operation of the device
before taking it back into use. Consult the manufacturer or call the service
in case of any doubt about safe operation or cleaning procedure after the
spill.
This device is designed for analyzing biological substances at biosafety
level 1 and 2 (BSL-1 or BSL-2).
lDo not use more hazardous substances due to limited possibilities
of decontamination.
lObserve the safety instructions for the substances used.
lObserve the instructions for decontamination and cleaning.
lAlways make sure to comply with national and international
laboratory safety instructions.
lAlways wear your personnel safety equipment.
There is a biohazard warning label at the back of the plate loading door
(visible when open, see figure below).
As supplied, the reader does not provide any particular protection against
the hazards evolving from aggressive chemicals, flammable or explosive
fluids, biological substances or radioactive substances. Therefore, it is not
allowed to use the reader with the above substances.
Caution when working with samples with higher acid concentrations! The
escaping vapors or spilled liquids can damage the device. It is
recommended to set the measuring times for acid samples only to the
absolutely necessary times. Do not leave the plate in the instrument after
the measurement.
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Warning! The user must ensure that no substances are spilled from the
wells, or preferably use a plate seal to prevent cross-contamination. When
using the dispenser, special care must be taken to ensure that the correct
plate is inserted into the reader. Also, the bottles containing the liquids to
be dispensed should be placed in such a way that they do not topple.
Avoid overfilling of the wells. Shaking of a plate with overfilled wells or at
too high speed or operating the stacker with overfilled wells can cause
spilling and contamination of the system.
The user is responsible for ensuring that the substances used are not
heated to the ignition or flash point. Although the maximum temperature of
the measuring chamber is 65 °C, the heating plate can heat up to 92 °C.
The user is responsible for venting possible vapors of toxic or corrosive
materials before they reach dangerous concentrations.
1.4.4 Mechanical Hazards
Gripper arm and gripper (with stacker only):
Warning!
The gripper clamps the plate at the sides when
gripping. As a result, there is a risk of finger
squeezing in this area. Keep away from the working
area of the stacker when the stacker is in operation.
Warning!
There are pointed pins on the inside of the gripper. This poses a risk of
scratching (and infection if the gripper is contaminated with hazardous
substances). Do not touch the pins and be very careful when working near
the gripper.
Warning! Do not attempt to stop or limit the stacker movement while the
system is powered on. Doing so might injure the user or damage the
system.
1 Safety
VICTOR Nivo – User Manual 11

1.4.5 Gas
Danger! Carbon Dioxide Gas (CO2) and Nitrogen Gas (N2)
In high concentration, carbon dioxide is harmful to health. Depending on
the concentration it can lead to headache, tiredness, and dizziness, in
higher concentrations even to unconsciousness, spasms, and death.
Carbon dioxide is hardly perceptible.
Nitrogen mixes easily with air. High concentrations of nitrogen reduce the
oxygen content in the air.
lIf using the VICTOR Nivo reader with external gas control option, follow
the installation and safety instructions in this manual and the safety
instructions provided with the gas control option.
lDo not install the system in very small rooms or without sufficient room
ventilation.
lInstall pressure controllers at the gas tanks to reduce the output
pressure of each gas tank to 2bar. Lower pressures can lead to
malfunctions, higher pressures could destroy the instrument or lead to
harmful concentrations in case of an instrument malfunction.
lKeep the plate door of the VICTOR Nivo closed to avoid the escape of
gas.
lGas supply must be switched off during downtime of the instrument.
1.4.6 UV Radiation
The instrument contains an UV radiation source, which is encapsulated in
routine operation. If the cover of the instrument is removed for service or
maintenance, the service engineer must unplug the power supply cord to
ensure no UV radiation is emitted.
1.4.7 Dangerous Substances
The service engineer must use protective clothing and ensure that the
instrument is cleaned and decontaminated prior to opening the covers, if it
has been introduced to biohazardous, corrosive or toxic materials.
The instrument must be cleaned and decontaminated prior to transport or
disposal, if it has been introduced to biohazardous, corrosive or toxic
materials.
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1.5 Laser Radiation
Classified according to standard
IEC 60825-1:2014 (wavelength: 680±3nm;
no accessible radiation)
Warning!
Use of the device other than those specified here may result in hazardous
laser radiation exposure.
The device includes a 3B classified laser (optional) which produces light at 680 nm.
The cover of the instrument should not be removed or altered in any way. Doing so
could lead to hazardous laser radiation exposure.
The VICTOR Nivo Alpha reader’s plate loading door and upper hatch are equipped
with safety sensors which immediately prevent the laser operation if any of the
hatches is opened during a measurement.
Inside the Alpha reader next to the laser unit there is a label for the class 3B laser
and next to the optical fiber there is a warning label for laser aperture.
There is a laser radiation warning label at the back of the plate loading door (visible
when open, see figure below). There also is a laser radiation warning label at the
back of the optics loading door.
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1.6 Instrument Type Labels
VICTOR Nivo Reader
Dispenser (optional)
Stacker (optional)
1 Safety
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2 Introduction
2.1 Instrument Overview
VICTOR Nivo
1. Power button
2. Plate loading door
3. Optics loading door
4. Dispenser module
(optional)
2.2 Measurement Technologies
The VICTOR Nivo Multimode Plate Reader can be used to quantitatively measure
light emitting or absorbing substances both from top and bottom side of the plate,
making it suitable for several different assay types including kinetic, cellular,
enzymatic and autoimmune assays.
The VICTOR Nivo offers up to six different measurement technologies:
lAbsorbance (ABS)
lFluorescence Intensity (FI)
lFluorescence Polarization (FP)
lTime-Resolved Fluorescence (TRF)
lLuminescence (LUM)
lAlpha Laser (ALPHA)
2.3 Contents of Delivery
The shipping box contains the following items (if any of the items is missing, please
contact the manufacturer):
lPacking list
lVICTOR Nivo Multimode Plate Reader
lExternal power supply and power cord
lEthernet cable
2 Introduction
VICTOR Nivo – User Manual 15

lWi-Fi router
lUSB stick (in VICTOR Nivo design) containing:
oPDF manuals (Installation Guide, Quick Start
Guide, Release Notes, UserManual, Software
Manual, Site Readiness document)
oVICTOR Nivo Filter List
lPrinted manuals (User Manual, Installation Guide, Quick Start Guide)
Optional:
lDispenser
lGas control option
lStacker module
lLaptop
lAdditional filters/dichroics
lTest plate
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16 VICTOR Nivo– User Manual

3 Reader Installation
Please install the VICTOR Nivo according to the instructions given in the VICTOR
Nivo Installation Guide which is provided with the reader.
If the reader is to be installed with the optional stacker module, see also chapter 7
“Stacker Module (Optional)”, page 47.
3.1 Hardware Requirements
3.1.1 Computer Hardware
lThe reader can be operated with any desktop PC, laptop or tablet.
lFor best user interface experience a screen resolution of 1920x1080 pixels is
recommended.
l4 GB or more internal memory is recommended.
lAny printer that is compatible with your computer can be used.
3.1.2 Control Device Power Options
1. Disable any sleep or hibernate mode and other energy saving options of the
control device and do not shut down the control device while VICTOR Nivo is
operating.
2. If the connection to the control device is interrupted (e.g. network disconnected,
control device switched off or browser tab closed), a running measurement is
nevertheless continued and its results are saved. You can re-establish the
connection and log in again.
3. During longer (kinetic) measurements it is recommended to operate the control
device using the power supply and not the battery.
4. Make sure that the time, date and time zone of your control device are set
correctly. These settings are also applied to the VICTOR Nivo and the control
software.
If you change any of the time settings on the control device, you have to switch
off the VICTOR Nivo, unplug it from the mains and then restart it to have the
time re-synchronized.
How to disable the sleep mode (described for Windows®10):
1. Open Control Panel >All Control Panel Items >Power Options.
2. Click Change plan settings to change the settings of the selected power plan.
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VICTOR Nivo – User Manual 17

3. Change option Put the computer to sleep to Never (for both “On battery” and
“Plugged in”, if using a laptop).
3.2 Software Requirements
The reader can be operated using any device having either Microsoft Windows,
MacOS, iOS or Android installed.
The VICTOR Nivo comes with a pre-installed user interface software that is used to
operate the reader and export the results. The user interface is accessed through a
web browser. The following web browsers are recommended:
lGoogle Chrome
lMicrosoft Edge (Chromium)
Microsoft Internet Explorer and Microsoft Edge (Legacy) are explicitly no longer
supported. Please make sure you are using the latest version of your browser.
3 Reader Installation
18 VICTOR Nivo– User Manual

Notice
Please check regularly for software updates on the PerkinElmer website:
http://www.perkinelmer.com/lab-products-and-services/resources/software-
downloads.html
Click on “Multimode Plate Reader” to go to the software downloads for the
VICTOR Nivo.
See also chapter 3.7 “Software Update”, page 27.
3.3 Reader Positioning
Place the reader on a flat and even table surface free of dust, liquids or acidic vapors.
Avoid places subjected to direct sunlight and vibrations. Ensure that the power
supply cord can be easily unplugged when needed. Use only the external instrument
power and power cord that is provided with the instrument.
3.4 Power and Communication Connections
Connect the power cord of the external power supply and the Ethernet network cable
to the reader:
1. USB-B port
Used for service purposes only.
2. USB-A port
Used for connecting the optional stacker module.
3. LAN/Ethernet (RJ-45)
Used for connecting the reader to the PC.
4. Power supply (24V DC )
Ensure that the local main power matches the power requirements of the
external power supply. The power requirements are printed on the power
supply.
Warning!
Use only the external power supply and power cord supplied with the
instrument.
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VICTOR Nivo – User Manual 19

3.5 Turning the Reader On and Off
lTo turn the reader on, briefly press the power button.
lTo turn the reader off, press and hold down the power button for 3 seconds until
the light of the button goes out.
3.6 Computer Configuration and Connecting the
Reader
The reader has a built-in web server for communication via a standard internet
browser. The software for controlling the reader is located in this built-in web server
and can be accessed via the browser. The server and the browser communicate via
standard TCP/IP protocol. The pre-set IP address is:
l192.168.0.2
There are two alternative ways to connect the PC and the reader:
lDirect communication with an Ethernet cable between control PC and the
reader.
Best suited if no other network connections are used.
lIndirect communication with the included Wi-Fi router.
Best suited for use with another network and for multi-user environments with
many control PCs.
3.6.1 Installation with Direct LAN Cable Connection
Connecting the cables
1. Connect the power socket on the rear of the instrument to the mains power
using the external power adapter and the power cord.
2. Connect the Ethernet port on the rear of the instrument to the Ethernet port of
your control device (e.g. laptop) using the LAN network cable (D).
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