PerkinElmer FL 6500 User manual

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PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
FL 6500/8500 Single Cell Peltier Holder
Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the FL 6500/8500
Fluorescence Spectrometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•Full software control
•Liquid cooling system
•N2gas purging available
Figure 1 Single Cell Peltier Holder [P/N: N4201029]

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Dimensions and Specifications
Peltier Temperature Controller 201
Physical Characteristic Specification
Power
100-240 VAC, 50/60 Hz, 380W
Temperature Range
-5 to 100°C (Maximum Internal Temperature)
Maximum Ambient Operating Temperature
40°C
Dimensions
295 (W) x 416 (D) x 283 (H) mm
(11.6 (W) x 16.4 (D) x 11.1 (H) in)
Weight
16.0 Kg (35.2lb)
Coolant Volume
1 L
N
2Gas Available
Liquid Cooling System
Magnetic Stirrer Control
Single Cell Peltier Holder
Physical Characteristic Specification
Number of Sample Cells 1
Dimensions 130 (W) x 267 (D) x 143.5 (H) mm
Weight 2.14 Kg
Temperature Accuracy ≤±0.1℃
Temperature Precision ≤0.1℃
Temperature Stability ≤0.1℃
Ramping Time (from 0 to 100°C) 5 minutes

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Safety Warnings
WARNING
AVERTISSEMENT
When this label is attached to an instrument it means refer to the
manual.
Lorsque cette étiquette est attachée à un instrument, il est
nécessaire de voir le manuel.
WARNING
AVERTISSEMENT
There is risk of receiving a fatal electric shock if the fuses are
replaced with the power cord connected.
Il y a un risque d’électrocution si les fusibles sont remplacés tandis
que le cordon d'alimentation est encore branché.
See the following figure for the location of warning labels on the back of the instrument:
Warning
FUSE
Avertissement
FUSE
Warning
To prevent electrical shock,
do not open cover.
Refer all servicing to qualified
personnel.
Avertissement
Afin d’éviter tout risque
d’électrocution ne pas enlever
ce couvercle.
Faire appel au service Après-
Vente.
Warning
For continued protection against electrical
shock, replace with same type and ratings
of fuse.
Avertissement
Pour assurer la protection contre le risque
de choc électrique, remplacez le fusible
seulement avec le même type et la même
valeur nominale.

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Configuration of Single Cell Peltier Holder
Part Name Qty (ea)
Single Cell Peltier Holder 1
Peltier Temperature Controller 201 1
Power Cords 3 (US, UK, EU)
Interface Cable (USB) 1
Spare Fuse (AC 250V T5AL) 2
Temperature Probe 1
Stirring Bead 1
Coolant Hose 1
Coolant (700 mL) 2
Waste Basket for Coolant 1
Waste Hose for Coolant 1
Hose for Coolant circulation 1
Macro Cell with PTFE Stopper 1
Description
Single Cell Peltier Holder
Figure 2 Single Cell Peltier Holder
a. Interface connector between Single Cell Peltier Holder and Peltier Temperature Controller
201
b. Coolant Inlet/Outlet Quick Coupler
c. N2Gas fitting
d. Interface slot for Temperature Probe
e. Cell Lifting knob

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f. Temperature Probe
Peltier Temperature Controller 201
Front View of Peltier Temperature Controller 201
Figure 3 Front view
a. Flow Gauge: Indicator of the flow rate of N2 gas
b. Coolant Inlet
c. LED Indicators: Display the status of the operation of coolant circulation, fan, pump, etc. (If
there is any problem in the components, the red LED will flash with the beep alarm.)
d. Display Pad: Display temperature of sample block
e. Stirrer ON/OFF button: It is used for controlling of the stirring
f. AC Power: Main power ON/OFF
Switch symbol
IEC 60417-5268 (2002-10) In position of a bi-stable push control
IEC 60417-5269 (2002-10) Out position of a bi-stable push control

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Rear View of Peltier Temperature Controller 201
Figure 4 Rear view
a. Air Vent Manual button: It is used to identify the cause of warning alarm that sounds when the
trouble occurs with Peltier. The causes of the alarm are as follows;
i. When air occurs in the coolant hose
ii. When the liquid coolant does not flow
iii. Lack of liquid coolant
NOTE:
Air Vent Manual button is recommended to be manipulated by the service engineer.
b. Buzzer: It makes an alarm sound. If there is any problem before the measurement or the
malfunction occurs during the operation, the buzzer beeps
c. USB port
d. Fuse: AC socket + fuse holder
e. Interface: Interface cable is connected to the Single Cell Peltier Holder
f. Quick Coupler of Coolant lnlet/Outlet
g. Quick Coupler of Coolant Drain
h. N2Gas Inlet/Outlet ports
NOTE:
N2gas is not always required (the tube for N2 gas is not supplied with the accessory).

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How to Fill or Drain the Coolant
How to Fill the coolant
NOTE: When the Single Cell Peltier Holder is installed for the first time, the enclosed coolant should be filled by
following this procedure.
1. Prepare the Peltier Temperature Controller 201 in a location that is compatible with the required
environmental conditions for the operation.
2. Connect the hose for coolant circulation to the Peltier Temperature Controller 201.
Figure 5 Connect the hose for coolant circulation
3. Connect the power cord to the Peltier Temperature Controller 201.
Figure 6 Connect the power cord
4. Remove the four Phillips screws on the Air Vent Manual cover using the screwdriver.
Figure 7 Remove the Phillips screws

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5. Push the Air Vent Manual Button on.
Figure 8 Push the button
6. Turn on the AC power switch of the Peltier Temperature Controller 201.
Figure 9 Turn on the AC power switch
7. Check if the LEDs of LOW LEVEL and FLOW SENSOR are lit on.
Figure 10 LEDs of LOW LEVEL and FLOW SENSOR
8. Open the lid of the coolant inlet on top of the Peltier Temperature Controller 201 and fill up the
liquid coolant using a funnel.
Figure 11 Lid of coolant inlet

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9. Maintain the amount of liquid coolant to a level where the indicator is located between the
‘L’ (low) and ‘H’ (high) mark in the scale on the left side of the Peltier Temperature Controller.
Figure 12 Coolant level
CAUTION
ATTENTION
Liquid coolant should be used as refrigerant. Do
not
use any water (tab
water, DI, etc.). If water is used as refrigerant, the Peltier Temperature
Controller’s components might be corroded, and its performance may be
deteriorated.
Use any kind of liquid coolant of normal grade that is available. (e.g.
liquid coolant including Distilled Water 70 wt%, Propylene Glycol 27 wt%,
Additives 3 wt%).
Le liquide de refroidissement doit être utilisé comme réfrigérant. N'utilisez
jamais
d'eau (eau du robinet, eau déminéralisée, etc.) L'eau risque en
effet de corroder les composants du contrôleur Peltier et de réduire les
performances de ce dernier.
Utilisez un liquide de refroidissement du commerce de qualité normale
(par exemple, un liquide de refroidissement à base d'eau distillée à 70 %
M/V, de propylène glycol à 27 % M/V et d'additifs à 3 % M/V).
10. After filling up the coolant, check that the ‘LOW LEVEL’ LED is not lit.
Figure 13 Coolant level
11. Close the lid of the coolant inlet.

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12. Turn off the AC power switch of the Peltier Temperature Controller 201.
13. Push the Air Vent Manual button off.
14. Fasten the four Phillips screws on the Air Vent Manual button cover using a screwdriver.
15. Install the Single Cell Peltier Holder referring to section Installation (see page 12).
How to Drain the Coolant
1. Turn off the Peltier Temperature Controller 201.
2. Remove the four Phillips screws on the Air Vent Manual cover using a screwdriver.
Figure 14 Remove Phillips screws
3. Push the Air Vent Manual button on.
Figure 15 Push the button
4. Prepare a waste basket.
Figure 16 Waste basket

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5. Turn on the AC power switch of the Peltier Temperature Controller 201.
Figure 17 Turn on the AC power switch
6. Connect the waste hose to the Coolant Drain port on the rear panel.
Figure 18 Connect the waste hose
NOTE:
The coolant flows out as soon as the waste hose is connected, so the other side of the waste hose should be
placed into the waste basket before connected.
7. The coolant will be drained automatically.
8. If the coolant falls below ‘L’ (Low level), the LEDs of LOW LEVEL and FLOW SENSOR are lit on.
Figure 19 LEDs of LOW LEVEL and FLOW SENSOR
9. When drain is completed, disconnect the waste hose.
10. Turn off the AC power switch of the Peltier Temperature Controller 201.
11. Push the Air Vent Manual button off.
12. Fasten the four Phillips screws on the Air Vent Manual button cover using a screwdriver.

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Installation
1. Prepare the FL 6500/8500 and Peltier Temperature Controller 201 in a location that is compatible
with the required environmental conditions for the operation.
2. Connect the power cord and communication cable of the instrument.
3. Loosen the knob to take apart the existing accessory.
Figure 20 Loosening the accessory fixing bolt
4. Pull out the cell holder by hand.
Figure 21 Pulling out the cell holder
5. Remove the front cover plate.
Figure 22 Front Cover Plate
CAUTION
ATTENTION
Make sure the instrument is turned off while installing this accessory.
Assurez-vous que l’instrument est éteint lors de l’installation de cet accessoire.

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6. After checking the pogo pin position in the sample compartment, place the Single Cell Peltier
Holder to be fit into the pogo pin.
Figure 23 Inserting the Single Cell Peltier Holder
7. Fix the front cover plate of the Single Cell Peltier Holder with the bolts.
Figure 24 Fixing the Front Cover Plate
8. Tighten the accessory fixing bolt again.
9. Connect the accessory interface cable of the Peltier Temperature Controller 201 to the interface
connector of the Single Cell Peltier Holder.
Figure 25 Connecting the accessory interface cable

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10. Disconnect the hose for coolant circulation.
Figure 26 Disconnecting the hose for the coolant circulation
NOTE:
If you disconnect the hose, the remaining coolant in the hose may flow out of the hose. Be careful not to
touch the coolant with your bare hands.
11. Connect the coolant inlet/outlet hosed and N2gas tube between the Peltier Temperature
Controller 201 and the Single Cell Peltier Holder.
Figure 27 Connecting the coolant inlet/outlet and N2gas fitting
NOTE:
N2gas is not always required. If no gas is required, the user does not have to connect N2gas tube.
12. Connect the temperature probe to the interface slot of the Single Cell Peltier Holder.
Figure 28 Connecting the temperature probe

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13. Connect the Peltier Temperature Controller 201 to the PC via the USB cable.
Figure 29 Connecting the Peltier Temperature Controller 201 with the PC
14. Connect the power cord to the Peltier Temperature Controller 201.
Figure 30 Connecting the power cord
15. Turn on the power switch of the FL 6500/8500 and the Peltier Temperature Controller 201.
CAUTION
ATTENTION
The air vent manual button should be off before turning on the main power of the
Peltier Temperature Controller and also during the operation since it could be a
problem for the communication between FL 6500/8500 and the Peltier
Temperature
Controller.
Le bouton manuel de ventilation doit être éteint avant de mettre sous tension le
contrôleur de température Peltier et pendant le fonctionnement, car cela pourrait
poser un problème de communication entre le FL 6500/8500 et le contrôleur de
température Peltier.
Figure 31 Turn on the power switch

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16. Check that the Power LED is shown as a blue light.
Figure 32 Location of Power LED
NOTE: Peltier Temperature Controller 201 can be compatible with various peltier cell holders (single or multi).
Whenever you exchange the existing peltier cell holder to another one, you should perform the “Auto
Tuning” setup referring to section Peltier Temperature Controller 201 Auto Tuning Setup (see
page 23).
17. Turn off the power after the experiment is done.
Setting USB Serial Port
NOTE: When using the USB cable, USB Driver has already been installed when installing the FL 6500/8500 and
Spectrum FL software, you do not need to install it again.
If communication by the USB is not established, change the port setting as follows;
1. In Windows 7, select My Computer →Properties. In Windows 10, press Windows
+Pause/Break on the keyboard.

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2. Select Device Manager.
3. Select Ports (COM & LPT) to scroll down. These are the devices currently connected to the COM
ports. USB Serial Port (COMx) is listed when the driver installation is completed successfully.
4. Double click on the USB Serial Port (COMx) of the Ports (COM & LPT).
5. Select the Port Settings tab and select the Advanced… button.

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6. Set the parameters as shown below.
7. Select OK.
8. If the Peltier Temperature Controller 201 fails to communicate with the PC, change the COM Port
Number by the following steps.
9. Open the Advanced Setting for COMx window again by repeating the steps 1 to 6.
10. Select the COM Port number list to scroll down it and change the COM port number to another one
which is not in use, for example COM 1 to COM 10.

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11. Make sure that the changed COM Port Number is applied and select OK.
12. After the port setting is changed, restart the computer.
Measurement
1. Install the Single Cell Peltier Holder by referring to section Installation (see page 12).
2. Close the sample compartment cover and turn on the instrument.
3. Double-click on the Spectrum FL software and select a measurement mode.
4. Check the recognition of Accessory and click the arrow icon.
5. Click Connect to connect the Temperature Controller 201.

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6. Set the parameters of for the probe, temperature and temperature display settings.
Probe Settings This function is used to adjust temperature. It is only used for the
manufacturing process, so do not modify the values.
Temperature settings
Monitor Select the temperature used to monitor during measurement. Options
include: Block, Probe 1 or Probe2.
Temperature(
℃)
Enter the preset temperature for the experiment.
NOTE:
Starting temperature of experiment needs to be set up in the
method window.
Range(
℃)
Enter the error tolerance range.
Goto Temp After completing the parameter setup, select Goto Temp. Then the LED for On-LINE is turned on and it will start heating up or cooling down to
the set temperature in the temperature settings.
Auto Tuning This function is used to minimize the temperature fluctuation at the target temperature. Refer to Pettier Temperature Controller Auto Tuning Setup
on page 23.
System Off This function is used to stop the heating up or cooling down to the set
Temperature in the Temperature Settings.
Temperature
Display Select which temperature will be displayed on the panel: Block, Probe 1 or Probe 2. Note that the selected temperature is only displayed in real time.
Save Settings Save the Probe, Temperature and Temperature Display setting
parameters.