PerkinElmer LAMBDA 265 User manual

LAMBDA 265
Users Guide
UV SPECTROMETERS

2 . Lambda 265 Users Guide
Release History
Part Number Release Publication Date
09931225 D September 2016
Any comments about the documentation for this product should be addressed to:
User Assistance
PerkinElmer
710 Bridgeport Avenue
Shelton, CT 06484-4794
U.S.A.
Or emailed to: [e-mail]
Notices
The information contained in this document is subject to change without notice.
Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no
warranty of any kind with regard to this document, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in
connection with furnishing, performance or use of this material.
Copyright Information
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translated into any language without the prior, written permission of PerkinElmer, Inc.
Copyright © 2016 PerkinElmer, Inc.
Trademarks
Registered names, trademarks, etc. used in this document, even when not specifically marked as such,
are protected by law.
PerkinElmer is a registered trademark of PerkinElmer, Inc.

Contents . 3
Table of Contents
Introduction......................................................................................5
Overview ...............................................................................................6
About This Manual..................................................................................7
Basic Theory..........................................................................................8
PDA UV-Visible Spectrophotometer....................................................9
Safety Summary...................................................................................10
Instrument Shipping Kit ........................................................................13
Conventions Used in this Manual............................................................14
Notes, cautions and warnings..........................................................14
Note, attention et avertissement .....................................................14
Contact Us...........................................................................................17
Safety Practices ..............................................................................19
Overview .............................................................................................20
Precautions..........................................................................................21
General Operating Conditions ................................................................22
Electrical Safety....................................................................................23
EMC Compliance...................................................................................25
Warning Labels ....................................................................................26
WEEE Instructions for PerkinElmer Products ...........................................27
Decontamination............................................................................28
Cleaning the Instrument.................................................................28
Fuses ..................................................................................................29
Lambda 265 Installation.................................................................31
Preparing the Laboratory.......................................................................32
Installation...........................................................................................34
UV Lab Software Installation.........................................................35
How to Install the UV Lab Software........................................................36
Interface Setup...............................................................................41
Setting USB Serial Ports ........................................................................46
Operating Procedure.......................................................................51
Procedure............................................................................................52
Accessories......................................................................................54
Accessories for the Lambda 265.............................................................55
Single Cell Holder...........................................................................55
Water Jacketed Single Cell Holder....................................................56
Variable Pathlength Holder..............................................................56
Advanced Transmission Holder........................................................57
Reflectance Module........................................................................57
Peltier Controlled Single Cell, With Peltier Controller..........................57
8 Position Multi Cell Holder .............................................................58
Water Jacketed 8 Position Multi Cell Holder......................................58
Autosampler..................................................................................58
Nano Stick.....................................................................................59
Magnetic Stirrer Assembly...............................................................59
Index ..................................................................................................60

4 . Lambda 265 Users Guide

Introduction. 5
Introduction

6 . Lambda 265 Users Guide
Overview
The Lambda 265 instrument runs using UV Lab software.
This manual provides instructions for installing and operating the Lambda 265 UV-Visible
Spectrophotometer and UV Lab software. It also introduces and describes how to use basic
features of the UV Lab software. For more detailed information on software features
including kinetics, bio analysis, color analysis and MCA, refer to the UV Lab software user
guide.

Introduction. 7
About This Manual
This user’s guide is divided into following chapters:
Chapter 1 Introduction
This chapter contains a brief introduction on the instrument, the conventions and warnings
used in the manual.
Chapter 2 Safety Practices
Important safety information is provided in this chapter.
Chapter 3 Lambda 265 Installation
Information on installing and re-installing your instrument should you ever need to move
your system is provided.
Chapter 4 UV Lab Software Installation
The complete UV Lab software installation procedures for the Lambda 265 is in this chapter.
Chapter 5 Interface Setup
Interface procedures for the Lambda 265.
Chapter 6 Operating Procedure
This chapter contains information on operating the Lambda 265.
Chapter 7 Accessories
This chapter provides information on the accessories available for the Lambda 265.
Index

8 . Lambda 265 Users Guide
Basic Theory
Electromagnetic radiation can pass through most organic and inorganic compounds.
Generally, the UV range is from 190 to 380 nm and visible range is from 380-800 nm.
Figure 1. Electromagnetic Radiation
When continuous radiation passes through a sample, some is absorbed by the sample. An
absorption spectrum can be obtained by monitoring the radiation that penetrates the sample
and reaches a detector. For every substance, the absorption rate varies depending on the
wavelength of the radiation. Data from absorption spectra can be used for qualitative and
quantitative analysis.
A molecule remains in its ground state when it is stable, but it can transition to an excited
state when light energy is absorbed. This is termed absorption. (Figure. 2) When the excited
molecule returns to the ground state, it emits heat, radiation, fluorescence or
phosphorescence. This is termed emission. A molecule whose functional group has a double
bond between carbons or between carbon and another atom undergoes transitions in the
UV-Visible range. Functional groups that absorb light energy are called Chromophores.
Figure 2. Energy Absorption

Introduction. 9
Once a sample absorbs light energy, the intensity of incoming light P0 (the energy from the
light source) reduces to P by losing some of its energy; therefore, we define the
transmittance of a sample as follows:
Figure 3. Sample Absorption
As a result of absorption by a sample, transmittance is defined as;
T = P / P0
And Absorbance is defined as:
A = -log T = log( P0/ P )
Absorbance is related to c (concentration) and b (pathlength of light) according to the
Lambert-Beer law and the equation;
A=εbc
Where, ε , the molar absorption coefficient of a particular molecule, varies with the
wavelength.
Although Beer's law has several limitations, it is widely applied to quantitative analysis.
Figure 4. Transmittance and Absorbance
PDA UV-Visible Spectrophotometer
The PDA Spectrophotometer has a multi-channel detector controlled by a microprocessor
which collects spectral data for several wavelengths simultaneously. In contrast, a PMT
Spectrophotometer has a single channel detector which collects data for each specific
wavelength in turn. Therefore the PDA Spectrophotometer is much faster and gives more
reproducible results than the PMT Spectrophotometer which depends on a rotating grating or
prism operated by a stepper motor to obtain individual wavelengths.
One feature of a PDA is an integrating photo-detector, which integrates charge depending on
the light intensity. The advantage of the integrating function is named Felgette's S/N
advantage or Multi-channel advantage.

10 . Lambda 265 Users Guide
Safety Summary
The following safety symbols are used on this product.
Warning Description
Always refer to the system manual when working near locations at
which the alert mark shown on the left is attached. If the operation,
etc., is performed without heeding the advice in the System manual,
there is a risk of personal injury. In addition, the equipment
performance may be reduced. Moreover, this alert mark is sometimes
used with other marks and descriptions indicating other dangers.
Il faut toujours voir le manuel du système lors de l'exécution de travaux
près des endroits où le panneau d’alerte montré dans la colonne à
gauche est placé. Il y a un risque de blessures, si le travail est effectué
sans respecter les conseils dans le manuel du système. En outre, le
rendement de l'équipement pourrait être réduit. Par ailleurs, ce
panneau d’alerte est parfois utilisé avec d'autres panneaux comportant
les indications de danger.
Warning
When supplying power to this equipment, connect the accessory 3-pin
power cord to a 3-pin grounded power outlet. If a grounded 3-pin
outlet is not available, use a conversion adapter and ground the green
wire. If power is supplied without grounding the equipment, there is a
risk of receiving a fatal electric shock and equipment damage.
Lors de l'alimentation de l’équipement, branchez le cordon
d'alimentation accessoire à 3 broches dans une prise d’alimentation
mise à la terre à 3 broches. Si une prise mise à la terre à 3 broches
n’est pas disponible, utilisez un adaptateur de conversion en raccordant
le fil vert pour réaliser la mise à la terre. Si l'alimentation est fournie
sans mettre l'équipement à la terre, il y a un risque d’électrocution et
de dommages à l’équipement.
“On” (power)
To indicate connection to the mains, at least for mains switches or their
positions, and all those cases where safety is involves.
"On" (Sous tension)
Pour indiquer le branchement à l’alimentation du secteur, au moins
pour les interrupteurs du secteur ou leur position, et tous les autres cas
qui concernent la sécurité.

Introduction. 11
“OFF” (power)
To indicate disconnection from the mains, at least for mains switches or
their positions, and all those case where safety is involves.
"OFF" (Hors tension)
Pour indiquer la coupure du secteur, au moins pour les interrupteurs du
secteur ou leur position, et tous les autres cas qui concernent la
sécurité.
Repair
Warning
The user cannot repair this equipment.
DO NOT attempt to open the case or to disassemble internal parts.
Only your PerkinElmer service representative with knowledge of
electrical fire and shock hazard should service this equipment.
There are high-voltage parts in this equipment presenting a risk of
severe injury or fatal electric shock to untrained personnel.
In addition, there is a risk of damage to precision parts.
L'utilisateur ne doit pas essayer de réparer cet équipement.
NE PAS tenter d'ouvrir le boîtier ou de démonter ses pièces internes.
Seul un représentant du service PerkinElmer qui est conscient des
risques d'incendie et d’électrocution devrait réparer cet équipement.
Cet équipement contient des parties à haute tension présentant des
risques de blessure grave ou d’électrocution au personnel non qualifié.
En outre, il existe un risque d'endommagement des pièces de précision.
Falling Over
Caution
This equipment should be used in the correct position. If the equipment
is turned on its side, etc., it will be unstable and may be damaged if it
falls over as a result of receiving a slight mechanical shock.
Cet équipement doit être utilisé dans l’orientation correcte. Si, par
exemple, l'équipement est couché sur un de ses côtés, il deviendra
instable. S’il tombe à la suite d'un léger choc mécanique, il pourra être
endommagé.

12 . Lambda 265 Users Guide
Warning Description
Changing Fuse
Warning
For continued protection against risk of fire, replace only with same
type and rating of fuse.
Before changing the fuses, ALWAYS remove the power cord from the
power-outlet and replace the blown fuses.
250V ~ T2.0AL indicates a time-lag fuse.
Pour assurer la protection contre le risque d'incendie, le fusible ne doit
être remplacé qu’avec le même type et la même valeur nominale.
Il faut TOUJOURS débrancher le cordon d'alimentation de la prise
électrique avant de remplacer les fusibles grillés.
250V ~ T2.0AL indique un fusible à action retardée.
Cleaning
Caution
Keep the equipment free of dust.
Clean the power line regularly; if dust accumulates around the power
pins, there is a risk of fire.
Keep the equipment clean so that the ventilation holds are not
obstructed. If the ventilation is obstructed, the system may overheat
and catch fire.
Periodically clean your equipment's case using a damp cloth.
Do not use abrasives, cleaning solvents or strong detergents, as they
may damage the finish or affect the reliability of the structural
components.
Gardez l'équipement exempt de poussières.
Nettoyez le cordon d'alimentation régulièrement. Les poussières qui
s’accumulent autour des broches du cordon d'alimentation pourraient
entraîner un risque d'incendie.
Gardez l'équipement propre de sorte que les orifices de ventilation ne
soient pas bouchés. Si les g
rilles de ventilation sont obstruées, le
système pourrait surchauffer et s'enflammer.
Nettoyez périodiquement le boîtier de l'équipement à l'aide d'un chiffon
humide.
If this equipment is used in a manner not specified by this manual, the
protection provided by the equipment may be impaired.
Si l’équipement est utilisé d'une manière non spécifiée dans ce manuel,
la protection fournie par l'équipement risque d’être altérée

Introduction. 13
Instrument Shipping Kit
The following table shows what is included in the Lambda 265 Spectrophotometer shipping
kit.
Item Name Qty.
Main
Instrument Lambda 265 Spectrophotometer 1
Accessories Power Cord (US/EU/UK) 1/1/1
Interface Cable (USB) 1
Fuse AC 250V (T2.0AL) 2
Wrench, 3mm 1
Document Pack CD 1
UV Lab Software CD 1
Single Cell Holder 1

14 . Lambda 265 Users Guide
Conventions Used in this Manual
Normal text is used to provide information and instructions.
Bold text refers to text that is displayed on the screen.
UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used
to show that you have to press two keys at the same time, for example, ALT+F.
All eight digit numbers are PerkinElmer part numbers unless stated otherwise.
Notes, cautions and warnings
Three terms, in the following standard formats, are also used to highlight special
circumstances and warnings.
NOTE:
A note indicates additional, significant information that is provided with some procedures.
Note, attention et avertissement
Trois termes, dans les formats standard suivants, sont également utilisés pour mettre des
circonstances et avertissements spéciaux en évidence.
NOTE:
Une note indique des renseignements supplémentaires et significatifs qui sont fournis avec
certaines procédures.

Introduction. 15
CAUTION
We use the term CAUTION to inform you about situations that could
result in serious damage to the instrument or other equipment. Details
about these circumstances are in a box like this one.
DCaution (Achtung)
Bedeutet, daß die genannte Anleitung genau befolgt werden muß, um
einen Geräteschaden zu vermeiden.
DK Caution (Bemærk)
Dette betyder, at den nævnte vejledning skal overholdes nøje for at
undgå en beskadigelse af apparatet.
ECaution (Advertencia)
Utilizamos el término CAUTION (ADVERTENCIA) para advertir sobre
situaciones que pueden provocar averías graves en este equipo o en
otros. En recuadros éste se proporciona información sobre este tipo de
circunstancias.
FCaution (Attention)
Nous utilisons le terme CAUTION (ATTENTION) pour signaler les
situations susceptibles de provoquer de graves détériorations de
l'instrument ou d'autre matériel. Les détails sur ces circonstances
figurent dans un encadré semblable à celui-ci.
ICaution (Attenzione)
Con il termine CAUTION (ATTENZIONE) vengono segnalate situazioni
che potrebbero arrecare gravi danni allo strumento o ad altra
apparecchiatura. Troverete informazioni su tali circostanze in un
riquadro come questo.
NL Caution (Opgelet)
Betekent dat de genoemde handleiding nauwkeurig moet worden
opgevolgd, om beschadiging van het instrument te voorkomen.
PCaution (Atenção)
Significa que a instrução referida tem de ser respeitada para evitar a
danificação do aparelho.

16 . Lambda 265 Users Guide
WARNING
We use the term WARNING to inform you about situations that could
result in personal injury to yourself or other persons. Details about
these circumstances are in a box like this one.
DWarning (Warnung)
Bedeutet, daß es bei Nichtbeachten der genannten Anweisung zu einer
Verletzung des Benutzers kommen kann.
DK Warning (Advarsel)
Betyder, at brugeren kan blive kvæstet, hvis anvisningen ikke
overholdes.
EWarning (Peligro)
Utilizamos el término WARNING (PELIGRO) para informarle sobre
situaciones que pueden provocar daños personales a usted o a otras
personas. En los recuadros como éste se proporciona información
sobre este tipo de circunstancias.
FWarning (Danger)
Nous utilisons la formule WARNING (DANGER) pour avertir des
situations pouvant occasionner des dommages corporels à l'utilisateur
ou à d'autres personnes. Les détails sur ces circonstances sont
données dans un encadré semblable à celui-ci.
IWarning (Pericolo)
Con il termine WARNING (PERICOLO) vengono segnalate situazioni
che potrebbero provocare incidenti alle persone. Troverete
informazioni su tali circostanze in un riquadro come questo.
NL Warning (Waarschuwing)
Betekent dat, wanneer de genoemde aanwijzing niet in acht wordt
genomen, dit kan leiden tot verwondingen van de gebruiker.
PWarning (Aviso)
Significa que a não observância da instrução referida poderá causar
um ferimento ao usuário.

Introduction. 17
Contact Us
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using
the part numbers quoted in the guides provided with the instrument.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for
information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your
order will be shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.

18 . Lambda 265 Users Guide

Introduction. 19
Safety Practices

20 . Product Title Hardware Guide
Overview
This chapter describes the general safety practices and precautions that must be observed
when operating the Lambda 265.
This advice is intended to supplement, not supersede, the normal safety codes in the user's
country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The
information provided does not cover every safety procedure that should be practiced.
Ultimately, maintenance of a safe laboratory environment is the responsibility of the analyst
and the analyst's organization.
Please consult all manuals supplied with the Lambda 265 and accessories before you start
working with the instrument. Carefully read the safety information in this chapter and in the
other manuals supplied. When setting up the instrument or performing analyses or
maintenance procedures, strictly follow the instructions provided.