PerkinElmer FL 6500 User manual

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PerkinElmer, 710 Bridgeport Avenue,
Shelton, CT 06484-4794, U.S.A
Produced in the USA.
FL 6500/8500 Water Jacketed 4-Position Multi-Cell
Holder w/Stirrer Installation Instructions
This instruction sheet describes the installation of this accessory which is used with the FL 6500/8500
Fluorescence Spectrometer.
NOTE:
Read these instructions before you install this accessory.
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
• Controls the temperature by circulation of liquid
• 4-position cell holders
• Built-in stirring mechanism
• Control stirring speed using Spectrum FL software
Figure 1 Water Jacketed 4-Position Multi-Cell Holder w/Stirrer (P/N:N4201027)

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Dimensions and Specifications
Physical Characteristic Specifications
Moving distance of one cell (mm) 12.8
Moving distance of four cells (mm) 38.4
Moving time of one cell (sec) 1.55
Moving time of four cells (sec) 4.3
Dimensions (mm) 190 (W) X 267 (D) X 98 (H)
Weight (Kg) 1.84
Environmental Conditions for Operation
Configuration of the Water Jacketed 4-Position Multi-Cell Holder
w/Stirrer
•Water Jacketed 4-Position Multi-Cell Holder w/Stirrer
Figure 2Water Jacketed 4-Position Multi-Cell Holder w/Stirrer Configuration
•Four Stirring Beads (P/N: N4202032)
•Four Teflon Blocks (P/N: N4202033)
Specification Comment
RT ~ 100℃Controls the temperature using Circulator

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•Two Male Connectors of Quick Coupling
Figure 3 Two Male connectors of quick coupling
Installation
1. Prepare the FL 6500/8500 Fluorescence Spectrometer to install this accessory.
2. Connect the instrument power cord and the communication cable.
3. Loosen the accessory fixing bolt and remove the current sample accessory.
Figure 4Loosening the accessory fixing bolt
4. Pull out the cell holder by hand.
Figure 5Pulling out the cell holder
5. Remove the current front cover plate.
Figure 6Front Cover Plate

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6. After checking the pogo pin position of the sample compartment, place the Water Jacketed 4-
Position Multi-Cell Holder w/Stirrer into the pogo pin.
Figure 7 Installing the accessory
7. Fix the front cover plate of the Water Jacketed 4-Position Multi-Cell Holder w/Stirrer with the
bolts.
Figure 8 Fixing the Front Cover Plate
8. Tighten again the accessory fixing bolt.
9. Connect the M1 and Stirrer cable of Water Jacketed 4-Position Multi-Cell Holder w/Stirrer to the
accessory communication ports inside the sample compartment.
Figure 9 Insert the M1 and Stirrer cables

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10. Assemble the tube with the male connector of quick coupling.
Figure 10 Assembling the Tube with the Male Connector
NOTE:
Tube size is recommended 3 mm-ID and 6 mm-OD and made of silicon which has thermal
resistance up to at least 100ºC.
11. Connect the male connector with the tube to the quick coupling mount of the front cover plate.
Figure 11 Connecting the male connector to the quick coupling
12. Switch on the instrument.
Calibration of Multi-Cell Position
Calibrate the beam position of the Water Jacketed 4-Position Multi-Cell Holder w/Stirrer when the Multi-
cell is installed for the first time or the beam position is observed to be incorrect.
1. Put the four Teflon blocks into each cell holder.

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2. Close the sample compartment cover.
3. In the spectrum FL software, click the Accessory icon to perform the calibration.
4. The following window appears.
Command Function
Total Step The total accessory number of steps is fixed at 27500.
Scan Speed Select the calibration scan speed. 30, 60, 240, 1200, 2400, 12000
nm/min. Default is 2400 nm/min.
PMT Voltage Enter the PMT voltage. Ensure that the intensity of the Teflon
block is not saturating.
Calibrate Used to perform the Multi-cell calibration.
Stop Used to stop the Multi-Cell calibration.
Cell Position Show saved steps about each cell position of the Multi-cell.
Save Used to save the calibrated result.
Load The last stored cell position value is loaded.
Move to Used for moving Multi-Cell position.
Select a cell position and click Move to button.
Reset Used to move to ‘0’ step of Multi-Cell position.

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5. Set the PMT voltage and click Reset to format the Multi-Cell steps.
6. Click Calibrate, and then the following dialog box will appear. Click OK.
7. The Multi-Cell calibration will start.
NOTE: If the peaks do not look as shown in step 8 or if any peak is saturated, click Stop, and then go back to
step 5 and lower the PMT Voltage value and resume the calibration.
8. When calibration is finished, click Save to save the result.
9. As the following message box appears, click OK.

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Measurement
1. Run Spectrum FL software as Administrator and select a measurement mode.
2. Check the recognition of Accessory.
3. Set up the measurement parameters in turn.
NOTE:
For more details of method setup, refer to Spectrum FL Software Users Guide.
4. As you click the Accessory tab in the Data Collection, the following window appears.
5. Select Water Jacketed 4-Position Multi-Cell Holder with Stirrer and select the cells to be used.
Then select the stirrer speed.
6. Set the number of samples, type and cell position for measurement in the Sample Table tab.
7. Click Save to save the method after setting up the parameters.
8. Put samples into the sample holder and close the sample compartment lid.
9. Select the Run icon.
10. Input the sample name and select OK.
11. Check the spectrum and results. Save or print the data if required.

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Troubleshooting
When the Multi-Cell does not move
1. Check the Connection status between the M1 cable and the accessory communication port.
When the cell holder position is not aligned precisely
1. Recalibrate the Multi-Cell holder if the light beam does not go through the center of the cell holder
hole.
2. If the intensity value is still low after the recalibration, the lamp of the light source may be
deteriorated and needs replacing. Contact your local PerkinElmer service office.
When the Multi-Cell does not reset
If the resetting is failed, the photo interrupter switch inside the Multi-cell Holder needs replacing.
Contact your local PerkinElmer service office.
When constant temperature is not maintained
1. Check if the water bath is working normally.
a. Check if the temperature of the water bath is maintained well.
b. Check if liquid is flowing well through the tube to the accessory.
2. Check if the tubes are connected to the connectors tightly.
When a magnetic bar does not work properly
1. Check the connection status between the instrument and the stirrer cable.
2. Test the stirrer’s speed in turn between Low and High.

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